LOCUS       DACDVT010000001        99945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDVT010000001 DACDVT010000000
VERSION     DACDVT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.001"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(554..2167)
                     /locus_tag="IWL52_000001"
     CDS             complement(554..2167)
                     /locus_tag="IWL52_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9776600.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="IWL52_000002"
     CDS             complement(2308..3216)
                     /locus_tag="IWL52_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9776601.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="IWL52_000003"
     CDS             3399..4523
                     /locus_tag="IWL52_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9776602.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4750..5667
                     /locus_tag="IWL52_000004"
     CDS             4750..5667
                     /locus_tag="IWL52_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9776603.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="IWL52_000005"
     CDS             5660..6493
                     /locus_tag="IWL52_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9776604.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="IWL52_000006"
     CDS             6576..7694
                     /locus_tag="IWL52_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9776605.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7688..9586
                     /locus_tag="IWL52_000007"
     CDS             7688..9586
                     /locus_tag="IWL52_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9776606.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIEKCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9580..10803
                     /locus_tag="IWL52_000008"
     CDS             9580..10803
                     /locus_tag="IWL52_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9776607.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWVRGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="IWL52_000009"
     CDS             complement(10889..12823)
                     /locus_tag="IWL52_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9776608.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13049..13705
                     /locus_tag="IWL52_000010"
     CDS             13049..13705
                     /locus_tag="IWL52_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9776609.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13779..14132)
                     /locus_tag="IWL52_000011"
     CDS             complement(13779..14132)
                     /locus_tag="IWL52_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9776610.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14324..15253
                     /locus_tag="IWL52_000012"
     CDS             14324..15253
                     /locus_tag="IWL52_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9776611.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15264..16100
                     /locus_tag="IWL52_000013"
     CDS             15264..16100
                     /locus_tag="IWL52_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9776612.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16348..17124
                     /locus_tag="IWL52_000014"
     CDS             16348..17124
                     /locus_tag="IWL52_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9776613.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17300..18043
                     /locus_tag="IWL52_000015"
     CDS             17300..18043
                     /locus_tag="IWL52_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776614.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18036..19616
                     /locus_tag="IWL52_000016"
     CDS             18036..19616
                     /locus_tag="IWL52_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9776615.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19867..20346)
                     /locus_tag="IWL52_000017"
     CDS             complement(19867..20346)
                     /locus_tag="IWL52_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9776616.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20362..21114)
                     /locus_tag="IWL52_000018"
     CDS             complement(20362..21114)
                     /locus_tag="IWL52_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9776617.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21231..21301
                     /locus_tag="IWL52_000019"
     tRNA            21231..21301
                     /locus_tag="IWL52_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21263..21265,aa:Gly,seq:ccc)
     gene            21662..22387
                     /locus_tag="IWL52_000020"
     CDS             21662..22387
                     /locus_tag="IWL52_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9776618.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22642..22908
                     /locus_tag="IWL52_000021"
     CDS             22642..22908
                     /locus_tag="IWL52_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9776619.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22905..23336
                     /gene="ruvX"
                     /locus_tag="IWL52_000022"
     CDS             22905..23336
                     /gene="ruvX"
                     /locus_tag="IWL52_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9776620.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23361..23666
                     /locus_tag="IWL52_000023"
     CDS             23361..23666
                     /locus_tag="IWL52_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9776621.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23747..24892)
                     /locus_tag="IWL52_000024"
     CDS             complement(23747..24892)
                     /locus_tag="IWL52_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9776622.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24953..25600)
                     /locus_tag="IWL52_000025"
     CDS             complement(24953..25600)
                     /locus_tag="IWL52_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9776623.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25794..26087
                     /locus_tag="IWL52_000026"
     CDS             25794..26087
                     /locus_tag="IWL52_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776624.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26131..26442
                     /locus_tag="IWL52_000027"
     CDS             26131..26442
                     /locus_tag="IWL52_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776625.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26439..27063
                     /locus_tag="IWL52_000028"
                     /pseudo
     CDS             26439..27063
                     /locus_tag="IWL52_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27363..27725
                     /locus_tag="IWL52_000029"
     CDS             27363..27725
                     /locus_tag="IWL52_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776626.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27762..28610
                     /locus_tag="IWL52_000030"
     CDS             27762..28610
                     /locus_tag="IWL52_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9776627.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28600..30075
                     /locus_tag="IWL52_000031"
     CDS             28600..30075
                     /locus_tag="IWL52_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9776628.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30341..30748
                     /locus_tag="IWL52_000032"
     CDS             30341..30748
                     /locus_tag="IWL52_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9776629.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30751..30966
                     /locus_tag="IWL52_000033"
     CDS             30751..30966
                     /locus_tag="IWL52_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776630.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30970..31350
                     /locus_tag="IWL52_000034"
     CDS             30970..31350
                     /locus_tag="IWL52_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9776631.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31484..31639
                     /locus_tag="IWL52_000035"
     CDS             31484..31639
                     /locus_tag="IWL52_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9776632.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31708..32181
                     /locus_tag="IWL52_000036"
     CDS             31708..32181
                     /locus_tag="IWL52_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9776633.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32178..33872
                     /locus_tag="IWL52_000037"
     CDS             32178..33872
                     /locus_tag="IWL52_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9776634.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33838..34023
                     /locus_tag="IWL52_000038"
     CDS             33838..34023
                     /locus_tag="IWL52_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776635.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34027..35202
                     /locus_tag="IWL52_000039"
     CDS             34027..35202
                     /locus_tag="IWL52_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9776636.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35195..36718
                     /locus_tag="IWL52_000040"
     CDS             35195..36718
                     /locus_tag="IWL52_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9776637.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36774..37058
                     /locus_tag="IWL52_000041"
     CDS             36774..37058
                     /locus_tag="IWL52_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9776638.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37045..37380
                     /locus_tag="IWL52_000042"
     CDS             37045..37380
                     /locus_tag="IWL52_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9776639.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37675..38301
                     /locus_tag="IWL52_000043"
     CDS             37675..38301
                     /locus_tag="IWL52_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776640.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39043..39879
                     /locus_tag="IWL52_000044"
     CDS             39043..39879
                     /locus_tag="IWL52_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9776641.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39944..40378
                     /locus_tag="IWL52_000045"
     CDS             39944..40378
                     /locus_tag="IWL52_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9776642.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40446..41768)
                     /locus_tag="IWL52_000046"
     CDS             complement(40446..41768)
                     /locus_tag="IWL52_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9776643.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41922..43562)
                     /locus_tag="IWL52_000047"
     CDS             complement(41922..43562)
                     /locus_tag="IWL52_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9776644.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43892..44362
                     /locus_tag="IWL52_000048"
     CDS             43892..44362
                     /locus_tag="IWL52_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9776645.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44991..46094
                     /locus_tag="IWL52_000049"
     CDS             44991..46094
                     /locus_tag="IWL52_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9776646.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46378..47058
                     /locus_tag="IWL52_000050"
     CDS             46378..47058
                     /locus_tag="IWL52_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9776647.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47148..48182
                     /locus_tag="IWL52_000051"
     CDS             47148..48182
                     /locus_tag="IWL52_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9776648.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48196..48531
                     /locus_tag="IWL52_000052"
     CDS             48196..48531
                     /locus_tag="IWL52_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776649.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48515..50995
                     /locus_tag="IWL52_000053"
     CDS             48515..50995
                     /locus_tag="IWL52_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776650.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51051..52610
                     /locus_tag="IWL52_000054"
     CDS             51051..52610
                     /locus_tag="IWL52_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9776651.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52607..53971
                     /locus_tag="IWL52_000055"
     CDS             52607..53971
                     /locus_tag="IWL52_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9776652.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54142..55212
                     /locus_tag="IWL52_000056"
     CDS             54142..55212
                     /locus_tag="IWL52_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9776653.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55227..55895
                     /locus_tag="IWL52_000057"
     CDS             55227..55895
                     /locus_tag="IWL52_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776654.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55915..56493
                     /locus_tag="IWL52_000058"
     CDS             55915..56493
                     /locus_tag="IWL52_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9776655.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56896..57807
                     /locus_tag="IWL52_000059"
     CDS             56896..57807
                     /locus_tag="IWL52_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9776656.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58063..58980)
                     /locus_tag="IWL52_000060"
     CDS             complement(58063..58980)
                     /locus_tag="IWL52_000060"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9776657.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59182..59619
                     /gene="zapA"
                     /locus_tag="IWL52_000061"
     CDS             59182..59619
                     /gene="zapA"
                     /locus_tag="IWL52_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9776658.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59677..60225
                     /locus_tag="IWL52_000062"
     CDS             59677..60225
                     /locus_tag="IWL52_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9776659.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60340..62700
                     /locus_tag="IWL52_000063"
     CDS             60340..62700
                     /locus_tag="IWL52_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9776660.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62787..63101
                     /gene="trxA"
                     /locus_tag="IWL52_000064"
     CDS             62787..63101
                     /gene="trxA"
                     /locus_tag="IWL52_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9776661.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63283..65061
                     /gene="uvrC"
                     /locus_tag="IWL52_000065"
     CDS             63283..65061
                     /gene="uvrC"
                     /locus_tag="IWL52_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9776662.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65206..66105
                     /locus_tag="IWL52_000066"
     CDS             65206..66105
                     /locus_tag="IWL52_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776663.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66098..67360
                     /locus_tag="IWL52_000067"
     CDS             66098..67360
                     /locus_tag="IWL52_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9776664.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67523..69421
                     /locus_tag="IWL52_000068"
     CDS             67523..69421
                     /locus_tag="IWL52_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9776665.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69720..70439)
                     /locus_tag="IWL52_000069"
     CDS             complement(69720..70439)
                     /locus_tag="IWL52_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9776666.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70776..71108
                     /locus_tag="IWL52_000070"
     CDS             70776..71108
                     /locus_tag="IWL52_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776667.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71095..73098
                     /locus_tag="IWL52_000071"
     CDS             71095..73098
                     /locus_tag="IWL52_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9776668.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73117..73587
                     /locus_tag="IWL52_000072"
     CDS             73117..73587
                     /locus_tag="IWL52_000072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9776669.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73608..74192
                     /locus_tag="IWL52_000073"
     CDS             73608..74192
                     /locus_tag="IWL52_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9776670.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74207..75193
                     /locus_tag="IWL52_000074"
     CDS             74207..75193
                     /locus_tag="IWL52_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9776671.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75183..75494
                     /locus_tag="IWL52_000075"
     CDS             75183..75494
                     /locus_tag="IWL52_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9776672.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75516..77297
                     /locus_tag="IWL52_000076"
     CDS             75516..77297
                     /locus_tag="IWL52_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9776673.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77290..78666
                     /locus_tag="IWL52_000077"
     CDS             77290..78666
                     /locus_tag="IWL52_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9776674.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGQPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78673..79308
                     /locus_tag="IWL52_000078"
     CDS             78673..79308
                     /locus_tag="IWL52_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9776675.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79338..80693
                     /locus_tag="IWL52_000079"
     CDS             79338..80693
                     /locus_tag="IWL52_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9776676.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80885..82279)
                     /locus_tag="IWL52_000080"
     CDS             complement(80885..82279)
                     /locus_tag="IWL52_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9776677.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82371..83342
                     /locus_tag="IWL52_000081"
     CDS             82371..83342
                     /locus_tag="IWL52_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9776678.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83339..84121
                     /locus_tag="IWL52_000082"
     CDS             83339..84121
                     /locus_tag="IWL52_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9776679.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84290..84661)
                     /locus_tag="IWL52_000083"
     CDS             complement(84290..84661)
                     /locus_tag="IWL52_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9776680.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84795..85238
                     /locus_tag="IWL52_000084"
     CDS             84795..85238
                     /locus_tag="IWL52_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9776681.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85281..86090)
                     /gene="yidA"
                     /locus_tag="IWL52_000085"
     CDS             complement(85281..86090)
                     /gene="yidA"
                     /locus_tag="IWL52_000085"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9776682.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86109..87476)
                     /locus_tag="IWL52_000086"
     CDS             complement(86109..87476)
                     /locus_tag="IWL52_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9776683.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87460..88290)
                     /locus_tag="IWL52_000087"
     CDS             complement(87460..88290)
                     /locus_tag="IWL52_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9776684.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88453..88890
                     /locus_tag="IWL52_000088"
     CDS             88453..88890
                     /locus_tag="IWL52_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9776685.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88966..89586
                     /locus_tag="IWL52_000089"
     CDS             88966..89586
                     /locus_tag="IWL52_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9776686.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89775..90281
                     /locus_tag="IWL52_000090"
     CDS             89775..90281
                     /locus_tag="IWL52_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9776687.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90298..91197
                     /locus_tag="IWL52_000091"
     CDS             90298..91197
                     /locus_tag="IWL52_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9776688.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91284..92168
                     /locus_tag="IWL52_000092"
     CDS             91284..92168
                     /locus_tag="IWL52_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9776689.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(92360..92431)
                     /locus_tag="IWL52_000093"
     tRNA            complement(92360..92431)
                     /locus_tag="IWL52_000093"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92396..92398),aa:Arg,seq:ccg)
     gene            92491..93825
                     /gene="rpoN"
                     /locus_tag="IWL52_000094"
     CDS             92491..93825
                     /gene="rpoN"
                     /locus_tag="IWL52_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9776690.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            94028..95065
                     /locus_tag="IWL52_000095"
     CDS             94028..95065
                     /locus_tag="IWL52_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9776691.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            95106..96107
                     /gene="gap"
                     /locus_tag="IWL52_000096"
     CDS             95106..96107
                     /gene="gap"
                     /locus_tag="IWL52_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9776692.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            96217..97407
                     /locus_tag="IWL52_000097"
     CDS             96217..97407
                     /locus_tag="IWL52_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9776693.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            97511..98266
                     /locus_tag="IWL52_000098"
     CDS             97511..98266
                     /locus_tag="IWL52_000098"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9776694.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            98382..99680
                     /gene="eno"
                     /locus_tag="IWL52_000099"
     CDS             98382..99680
                     /gene="eno"
                     /locus_tag="IWL52_000099"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9776695.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        33162 a        17067 c        20603 g        29113 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcca
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttt agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaaatgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggtt agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcatct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tttcacaata
    12961 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    13021 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    13081 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    13141 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    13201 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    13261 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    13321 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    13381 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    13441 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    13501 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    13561 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    13621 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    13681 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    13741 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    13801 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    13861 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    13921 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    13981 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    14041 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    14101 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    14161 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    14221 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    14281 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    14341 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    14401 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    14461 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    14521 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    14581 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    14641 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    14701 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    14761 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    14821 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    14881 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    14941 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    15001 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    15061 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    15121 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    15181 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    15241 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    15301 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    15361 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    15421 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    15481 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    15541 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    15601 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    15661 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    15721 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    15781 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    15841 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    15901 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    15961 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    16021 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    16081 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    16141 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    16201 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    16261 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    16321 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    16381 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    16441 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    16501 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    16561 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    16621 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    16681 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    16741 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    16801 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    16861 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    16921 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    16981 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    17041 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    17101 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    17161 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    17221 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    17281 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    17341 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    17401 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    17461 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    17521 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    17581 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaagaaa agcgtttagc
    17641 agaattgaca gcgctttttg atatgtatag tcatcaaaat gaaacaatcg caagtttttc
    17701 acatggaatg cgtcaaaaaa cgattttgat cggcacgttg ctgccagatc cagatatttg
    17761 ggtattggat gaacctttgc aaggacttga tcctcaagca gcatttgatt tgaaagaaat
    17821 gatgaaggct catgccgcaa aaggaaaaac agtcattttt tctacacatg tattggatac
    17881 cgcacaacag ttatgcgata aactggctat tttgaagaaa ggtgaattga tttatcaggg
    17941 ggcagttagt gaattattga atggctcacc tgatgaaacg ctagaaaaga tttatttgaa
    18001 aatggctgga aggcaagcga caggagatgg accgtatgta taagcgacaa ttattagaat
    18061 taattcgagt taatctttta tatgttaatc cacagctcac gaataaagca agagagaaag
    18121 gaaaaaaggg aaagcgatta actatctatc ttttgtctca aaatcttttt tcagggctga
    18181 tttttttact gatttacgga tttgctatgt ttgctatgaa ctttgttaaa ttgccaggtt
    18241 tctttactta ttacgtggca ctttttggta tgttaggttt aactcaaagt atatcagtca
    18301 tttataatat cttttttgaa ggaaatgacc tgcaagtatt tttgcctctg ccttttggac
    18361 aagaacaggt tttttcagca aagattttgg tagttgcttt gaccatcatt ccttttgttt
    18421 ttccgcttct agtcatgttt attttaaccg gatggcgagc tgatctaggg attttgctta
    18481 ctatcattat ctcaatcctt ttgttttctc tatttttgat tattcttttt tgtctggcta
    18541 gtttgattgt ttttggtttg gctcgaacag cagtgtttaa aaaacataga aaaacagttt
    18601 ctagtatctt gcttggcagc tcgatgctga tcgcatttgc tggaatcatg tggatgaatc
    18661 atcaaacggg gactattgaa aatgagttaa cagatcggca tccgatttcg atattattgc
    18721 cattctatga tgctgccagt cagccacttt ctcaacaagg tttattgggt tggggaatac
    18781 tagctatcac ggctctggga tttttccttg ctgtaaaaca gctgatagta ccaaagctta
    18841 cagagcaact gactgattca aacagtgagt tcaaaataaa aagaacccat aaaaagaacc
    18901 aaaatgtgca tcagctttta tttagttaca atgctcaatt agtaaaagaa cctaatttga
    18961 ttatgcaagt tctttcttct tccttgttaa ctccaatcat ttttattctt gcttttgccc
    19021 taggaggcga aataaaatta actgatttag ataataagtt aatcggtgtt gttttcctgg
    19081 tagggatcgc attggctttc ttaacagtta atcagacttc ttttatcagc aatcttattt
    19141 ctttggatca agaaaatttt ctgtttatcc gttctttgcc aatctctatg aaccaatatt
    19201 taaaagaaaa gttcagattc ggatggatat tgcagagttt tttaactggt gggatcgcat
    19261 tattaagcgg actgtctttt cagctgcctt tgttttttat ccctagcttg ctatttggtt
    19321 ctttattcgg ctgctactta ttgtcactac gttatttcgc tagagattat cggttgctgc
    19381 tgctgaactg gaccaatatt aaccaattat ttaacagagg ttcaggaagt ttgggtttag
    19441 tcgcaactat gatgggttca ttgatcatca gtattattct tatcgtctta tacggaatgt
    19501 tggctttgtt ttatccattt tggttagtga atattcctgt gatcggttta gttctaatac
    19561 tttccgctct atggatcgtt tattatcaac gaacgttttg gaaaaaattt gaataaagaa
    19621 gaggggccgt gacaaaaatc atgtcgcagc ccctttttta cagatagagg gtgttcaaaa
    19681 gtcagtttct cagtattaag tcaattttat gtaaaaatgt gagaagctat ttttacaaaa
    19741 ctttttttaa tactcataat tacttcttat tgctcctgcg gttacttgtc gcaatgtttg
    19801 tgagatattt tgtcatcgct caaggctgaa cgtctctttc acaacctttt ctttgttaca
    19861 cttcgtttat ttaaaaagcc gtttttttcg tttcatcatg attgcgtcac gctttacttt
    19921 gaaattacgg atgttatggt taaagacttc tgataataca agacctaaag cgattgctcc
    19981 agcaatcata attgcttgga ccgcataatg agccgcttct cgataggatt cagtaacgag
    20041 atttcgaacc gcttgataag ctaaaccacc agggactaga ggaaccatcc cgggaatatt
    20101 gaaaatcgtt acgggcattt tcaaacgttt agagaaaaga tcgctgatga atgctactcc
    20161 taaagaacca agcaatgaac caaaagcagc agatccgcct atttgaacac agatccagta
    20221 gatcatccag ccaaaagcac cagaaagacc acaacaaatc aatgaacgcc taggtacatt
    20281 tgtaataacg gcaaatgccg cagtcgctaa aaaactaaaa gaaaactgaa taaatatcgt
    20341 ccccatactt gtgttccttt cctaataaaa aagctggaaa ataaaagcaa tcgcaaaacc
    20401 gatcatcgta gcagtcatca tcgcttctgt tccgcgagaa actcctgaga cataatgtcc
    20461 agctagcaaa tcacggatag cgttcgtaat ttgaactcca ggaactaaag gcatcacaca
    20521 tccgataatg attagatcct gattgcttcc taagccaaaa cgtacgctgt agatagcggc
    20581 tatgccaatc aataaagaag ataaaaattc cgataaaaat ttgattctaa aaaatttcaa
    20641 actatataga taagctgcgt atcccaatcc tccaatgact aaagtcacag gtaaatcggt
    20701 catctttccg ccaaataaca ccataattgt tccactgatc actgccgcgc taacgatttg
    20761 caaccagaca ggaaagaaag aacggtctcg gtcgattgct tgcagttcgc agtaaagttc
    20821 ttctaatgtt agctctgaag caacatattt acgtgaaaga tcatttactt ttactacttt
    20881 ttctaaatta atcgaacggt tggtaatttg ttccattcgg atggtcgagg tacgatcaag
    20941 accaataaag agaccggttt gagtgacata gctgaccaaa cggaaatttc cagaagctaa
    21001 agcaattcga ctcatcgtat cttctacccg atacatctcc gcattgcttt ccatcatgat
    21061 tttcccagcg agtaaacaag tgtccagcaa cagatctaat tcttttcccg tcatagcatc
    21121 ttctccaata attgattgat tagttttatt ttactacaaa acataccaga agcgtaaaga
    21181 ataaaaaaac ttgaggaagg aataaaagag gcgtatactg tttttgtatt gcgggtgtag
    21241 tttagtggta aaatcccagc ttcccaagct gatgtcgcgg gttcgattcc cgtcacccgc
    21301 ttaaaatagg ttgtgaaaaa gctgctctgc aacaagtaat aagaacagcc aagcaaaaac
    21361 aacttcttat gtttttaact tggatggact tattaccgca gatgcaagct tttgcgcacc
    21421 gtttaccaag cggttgtgaa atcagtgtat tgtcttgagt atgaagaaaa tttttgaata
    21481 ataataaata ccttacagat agagtggtga ctgattagag acgaggaatc cgtctctttt
    21541 ttgtattttt aagccatctt aaaacaggtt ttattttttg ctgtttctat ttctttctag
    21601 tatttattta taggggaaca tatgttaaga tttagggagc tgttagtaaa ggagtaaaga
    21661 aatgagcaaa aaaacaacca aggcgatcgt ggggatcgtc gctgctgtga ttgcaggggc
    21721 attcggtgtt tccaccacac aaaaagattc agatatcttt cagcagatta caactatttt
    21781 tgattccaca ccacaagctt ctcaagtagc ggatgtgcca gaatatgatg gaaaacatca
    21841 agaaatagaa atcaatggga atcagccgga atttacaaaa gaggatctta gcctttccaa
    21901 aggaacatgg gaaagctatt caaatattga taaattgaac agagtaggac aagccaatgc
    21961 tatgcttgga aaagatttgt tccctactga aaagagagag cctttgtata ttgacccgac
    22021 aggttggaaa caaaagaaat taagtgacgg gcaatggtta tataatcgca gccatttaat
    22081 tggtttccag ctgactggtc aaaataacaa cattaaaaac ttgatgacag ggacacgttc
    22141 gttgaatgct ccatacatgc ttgctcacga aaatgatatt gcggcatata tcaaagaaac
    22201 aaatcatcat gtacgctacc gtgtcactcc tcattttgag gggaatgaac tggtagccag
    22261 gggagtgcaa ttggaagcag aaagcattga agacaaaaaa attgaattca atgtctttat
    22321 ctacaatgtt caagatggct acacgatcaa ttatgagaca ggacaagcaa caaaaacaaa
    22381 aaattagtag cagctattcg aattttttat gaatattatg aaaaaattgt gtaaaaacat
    22441 acattcaaag aaatatagcg tatactttat gaaaagagta cttttgataa agctacagaa
    22501 aaaaggaaaa aaacatacaa atagtgtttc ttaatttatt cggaagtttg gaatatttgt
    22561 ttgtagtttt ccctttcttc gagtaaaata aggttgtaga aggtaagctg gaattacctt
    22621 gcgggaaaga ggtgtagaat catgggtttt acagatgaaa ccgtacgttt tggttttgac
    22681 gatagtcgca aaaaagaagt gagcgaaaca ttagcgattg tttatcgggc tttggaagaa
    22741 aaaggctaca atccaatcaa tcagattgtc ggctacttgc tttctggaga cccggcctac
    22801 attcctcgtt atcgtgatgc tcgaaatttg attcgccgtc acgaacgcga tgagatcatg
    22861 gaagtaattg tcaaggacta cctctcaaat catggagtga atttatgaga gtaatgggct
    22921 tggatgtcgg ttctaagaca gtaggtgttg ctatcagtga cccctttggc tggacagctc
    22981 aaggagtaga gatcattcgg atcaatgaag cagaagaaga gtttggcttt gatcggttga
    23041 aggaattagt agcacaatat gaagtagacc ggtttgtagt tggattacct aaaaatatga
    23101 acaattccat cggaccaagg gcagaagctt ccatgtcata cggagaaaag atcaaagaac
    23161 tattctcctt gccagttgat tatcaagatg aacgattaac cactgttcag gcagaacgta
    23221 tgctagtaga gcaggcaaat acttcaagag ccaaacgaaa aaaagtcatc gataaattag
    23281 cagctgtgat gatcttacaa aattatttag acggctcagg taagcaatta ttttaataat
    23341 aaaagaaaag aggtaatctt atgacagaac ataaccattc ccacgaccat gaaggacacg
    23401 aacacatcac attggtggat gaccaaggaa atgaaacatt atatgaaatc ctactaacag
    23461 ttgacggaca agaagaattt ggacgtaact atgttctttt atatcctgct ggtgtttcag
    23521 aagatgaaga tgtagaatta caagcttatg catacatcga aaacgaagat ggaacagaag
    23581 gcgagttaga acaaatcgaa acagacgcag aatgggacat gattgaagaa gtattcaata
    23641 cattcatggc ggaagaagaa gaatagacta taaagctagg agaaaccttg atatgatagg
    23701 gtttctcctt tttttatttt ttactgtatg ccatattgta tgccatctag tcaatattta
    23761 agtgttgtac taatttttca acagtctttt ctttcgtttc ttttgccaag tgaccgtaac
    23821 aatcgatcgt ctgactaatt tttgtatgcc gtaatctagt ttgaacttct accatagtag
    23881 ctccagcgtc caagagcatt gaagcatgag tgtgtcttgc tacatgcaca ccgagaaatg
    23941 gaaccatagc tttttcacaa atagtaatca atcttgcata aatagcgtgg tgtgtgatta
    24001 gttctttata gataccacaa aaaatcatag tcggatcagt tacaccaagt gtcagcatat
    24061 atttccgctg attaatctgc cattttttta atatagaaat tgttctttca tctagtggaa
    24121 gaaccccagc actacaatca gttttaggat cactgatttt atagccattt ttggattggc
    24181 ttagtgtttt attgatggtg accgtatttt cttgaaaatc aatgtctgac caattcaatg
    24241 caagggcttc actgatccta accccgctat aaaagagaaa gcgtagtaac gttttatcaa
    24301 agtcgtttcg ataagtttca ggtttgctgt ttaggtaatc aaacaaggtg atcagttgtt
    24361 ctttactgta aactttgacc ttttctttat gcttagtggt ggttctttta ggcattaata
    24421 cgttgtctag tggattagtc tcaataatcc caagattgat agcgtagcta aaaatacgct
    24481 tagaagttga atgaagtacc ttgtattggt cagtagtttc agcccagcta tttacaattt
    24541 gttgggcttt ttttaccgtg acttcattca cacgaagatt tccatatttt ggaagaatcc
    24601 actttctaaa cttgatttca gttttcatga aggtggcttc tcggacagtg gttttatatg
    24661 tttctagcca caagtcataa acttctttaa acgtttcgtt gttcgctttt ttaataccgt
    24721 ttttatcgta gtccacttgc aagcgtgaca aggcaagctg ggcttctttt tttgttttgt
    24781 aacctcgtct acgaacctta tcaggtttgc cagtcatttt gttcattcca agataggctt
    24841 catagagcca aaatttacct tttttagttt catattgctt gaatgttgcc ataatagttt
    24901 tccctccata gtgtagcaag ccgtgggggc ggttggagga ggcagtttat tattattttg
    24961 aatagtgttt atttagctca gtaacaagag ctgtgaactc ttttagaaga tcattttcat
    25021 aaaattcttg atactctttt ttctcatctg tatttttttc tgaatcatta attaatgagt
    25081 catatcgtaa aagcgtattt agaagagctg cgagataagc aattggggta ctcccgtcag
    25141 gaaattcaat tcgagatcct gttttaagat attttattgc gtttgttaaa taaatcttct
    25201 gtgagacagt caggttatct tcttcgatgt tattgatagc attagaaaga ttagaatcaa
    25261 ttatagagtt atttttttca atagtttttt tcaattgctt ttcaagcaca tttcgcttaa
    25321 tttcgggcat catatgcata tctcttaatg tataagatcc gtttaataga taggtagtgg
    25381 atacatctaa tacctttgaa agttgtttaa gcctggtgtt attgggaaga ttatagccat
    25441 tttcccaatt actaacggca cctttactgg ccggtgggga taatttacct ccaagttgtt
    25501 ccattgagag tcccttttca attctagcca gtctaattct ttgtcctgtc tccactgctt
    25561 cggggtattt ttttctagcc acattttcac caccattcat atattttctc tactatatca
    25621 cgatttaaat atgtgtgaaa cataaaagtt caaaaaagta tgttaaaatt cttgcaaatg
    25681 aaaaggtatg atggtatatt gagtttataa gaggtataga aatgtattca agagcctctt
    25741 gcattttttt aaaatctttg tatagaaatg tatgctaacg aggggggata aatatggaat
    25801 taaaaattga caaactcacg cttgatgatc cagcttttaa aattattgac gatcgagttg
    25861 ttcaacaagt agagcaaact caaggaacgt tactttcatt attagaaaaa aagtaccagt
    25921 ttccaaaata tatgaacaag gcacaagctg ctaagtatat gaacgtttca tacaatacta
    25981 tgatgcagaa atatgttcct aacggtttaa agttgattat agttgatgga gtcgtaagaa
    26041 tttcacaaga tgaatgtgat cgctttatgg aagaatacca aaaataaacg caagccgtgg
    26101 gggcaaaggg attaatagga ggaaataata atggaatcga cacaaaataa agcaatcgaa
    26161 aaagtattat caacaattct tacagaagac acagcggcag aattagcaaa cttggaaggc
    26221 aaagcgctag aagaaacgtt tgaatgtttg tacgaacaaa tggactatca aaaattgcta
    26281 ccacaagaac caacggtaag cggagtattg cgtggcttga acgatttagt acaagcagag
    26341 tttaaagaac gactttctat tgaagagtat caagaaattt tgtatcagca agttgagttg
    26401 ttagcgagct tgctagggct tgaactggag gacgttgaat gaaaactgaa atttggaacg
    26461 gacacattat aagatttgtc gatattaatg atgaatggtg ggcagtggcg aaagatgttg
    26521 cggaagcgtt aggtcttaaa caggttacta gagccattca ctctttgcct aaagatgggg
    26581 ttactactag taaggtcatc gattcattgg gcagaacaca agatgtaaac atcatcaacg
    26641 aaaaaaaata tttaccgcat ggcattcaaa agccgtaaaa aagaagccga ggcatttcaa
    26701 gactggattt tcgacatcat caaagaacta cgccagtcca caggacttga aggctttcaa
    26761 gtattccgca tgctagacaa agaacaccaa aaagaagcaa tgactaaatt aagccacgct
    26821 attactgaac caaaaccagt agactacatt aaagcgaatg tgatagccaa taaagctgta
    26881 tcaactattt atggtcattc caaaatggta aaaaagaaag atatgactcc tgaaatgttg
    26941 gttgatcgtg aacctatttt agatgagaca gtagaattaa tgactgttaa agaaaaatat
    27001 gggttgcagt tttcagtgag tgagaaaatt tataatcgtt ctgctgaatt gcagacaact
    27061 taggagcatt aacatgatcg agaacaaaga aaaagcctta tccataacag tcttggcgga
    27121 caggataaag gcaacttaat tgggataaaa cccgatattc tttgttccga ttataccata
    27181 ttaaaaaatc ggagggaacc attatgaaac gaaacttaat tataggaagc attttaatcg
    27241 gcggaatttt cggcttatta ccattaattg caaaaatagc tatctttgca ggattagcgt
    27301 tatatctctt cactgcttat gacgagtacg actatcaatt aagaactgga gggcataagt
    27361 agatgtacaa aacaaaacta ttaaaccaac tcgatagcct tgaattagag gaaattaatc
    27421 aagggatcgc tgaactagaa aataatatcg ggaaaaccta ctttggaaat tcattcaatg
    27481 aaaaactaac agttttgtat gtactcaaaa aacatgcaga acacaaaata atttgtcgag
    27541 aaatcaacga gctaaaaaat cagattttaa ccgcatggtt aaacattact gatatgcaag
    27601 aagcaagggt gaaaacattt aatacatggg taaagtatca gaatcaatta aaaggtgctg
    27661 aatttgttcg ggatgggttg aaatacgaac ttgagcagtt gaagctcatg gaggtgtctg
    27721 aatgatttat accagtattt tgaaaaagga ggagaggttt attggcaaat aattatcaag
    27781 cagcaacgga attacaaaag tcgggatggt caatttatcc cttatctcct ggaactaaaa
    27841 ttccaataaa aggctctagt ggttggaatg atgcaactaa cgattctaat caaattaatg
    27901 aatggtggac ggaagatgca actagaaata ttgggttaat gcttgaagat catcaaatgg
    27961 tggtagttga cctagatcaa cattctgaaa cacgaaacgg cgttgagaat tataaaaaac
    28021 ttgcttcaat gtatgatcca tttccttcta cttatacgga aatcacacca ggtaaaggaa
    28081 tccatttctt ctttaaaatt cctgatggtg taattatcca acaagaaaca ggtgcatttg
    28141 ctgatatctt cgggcgtgat gataaaggta aaagtttaag cgggattgat attatcacta
    28201 aaggagttcc aatagcgcca actatgatta tttcaagaag tgttgggaga gcatatgaac
    28261 ctattgaaaa cgtatctcta aataatattg caccagctcc cgattggcta gtgaacctac
    28321 tgctaaaaaa accgcaagaa ataaagactc aataccgacc cgtagcaaaa acagggacag
    28381 caaaaaaatt agatatgatt gttcaaggcg ctggcgaagg tagtagaaac gatcatttaa
    28441 ctaagttatg cggttggtta ctatggcacg gtgtagataa tcaaacttta gcagaattga
    28501 tttatacagc aaatgcgtac aatgaaccac cgctaaaaga taaagaagtg aatcaaatta
    28561 ttcgatcgat gattaaaaaa gatttgaggg ggaaagctca tggctcataa gcataaatta
    28621 gatagtattt tagactttcc cgaagccagc gagcgtgaag acaatatcat tgaattgaaa
    28681 acctggatgt cacgattaag atgcaataaa gacgatcaga ttaaaagtaa cagtgttgtg
    28741 aatgcagaat tgattttaac caatgatagc aatttagctg gaacaattgc ttataacgaa
    28801 tttagcggat acattcacct attaaaagat tctccttgga tcaatcgtac cgctggagaa
    28861 tgggaagaca gttttgagga tgccttaacg gcatatattg aagaaaacta caatgtagtt
    28921 tttgatgaca acaaaataca caaagcagta gtaaatgtgg ctagaaagaa tgttttcaac
    28981 ccagtaaaag aacgtatcga aaaagttaaa tgggatcaac agcctagact ggaaacactg
    29041 tttattgacc ttttaggggt agacgacaac ctttacacac gagaagtaac aaaacgttgg
    29101 atagtcggat cagtcgcacg tatctataaa ccaggaataa agtttgagat cgttccagtt
    29161 ttggatggac cgcaagggat tggtaagagt accgttccag cattacttta cacggatgat
    29221 tttttcaccg attcattaga ttcgctaggt gaaaagaaag atgattatat gcagcttcaa
    29281 ggcaatgtaa ttattgaact tggagaatta tcttcaatga acaaaactaa aattgaacag
    29341 ataaaaaact tcatcagtgc caagatcgat aaaattagac ctccttatgg tcggaatgtc
    29401 atagcttggg caaggcaatg tgtatttatt ggaacgtcga atgatgggca gtatttgaaa
    29461 gatgatacag gaaaccgccg tttttatcct ttgccttgta agaataagcc aaagatgaat
    29521 ccgtttaaaa caaaggatga atattttctt caagtgttag ctgaagcaaa ggtgcttttt
    29581 gataaaggac aacgaatcta ttttagtccc gatgaagata aagaagtctt agaaattgca
    29641 aaagactatc aagaggatgc caagatggag aatccaatta aagaagcaat cactaaatat
    29701 ttggaaatgg aaatcccgta taaatgggaa gaagctccag cgtgggcgag acgttcttat
    29761 tatcagaact atcctgacag taaatgtaat gagaaaaatt tacagcattt ctataatgga
    29821 gctactatgc aacaagagtt ttatttagtt gatagcgtac tcaccgctga tattttggaa
    29881 gctgtttttg ataaacaagc aaaggattta ttgaatggtc gatcagatgc agaaactaaa
    29941 aagattgcct tgattattag cagtattcct ggttgggaac ggaagcaact gccaaaaaga
    30001 aataaacgac gaggatttta caataagtca aatgcctatg aaaataaaaa tcgagctgga
    30061 aagtacaaaa agtaacgggt agtaacgggt cagtaacacc tagcccgtta ctagtttacc
    30121 cttactgccc caagggatac agcaatttgg taacggggta acaccttatt tccttaaaac
    30181 ttttgaaata gttagctaat atgctgggga ataatgttac tggattttac ccgttacctg
    30241 ttaccaaaac gctagaaccc ttgtccccgt tgggattgag tggtaacggg tacctgttac
    30301 gtaggtgtta ccacgtgtta cgaatcaagg aggaaagtaa atgctcaata ttatttcaac
    30361 aaacaaagca ccgaactttc aatatacaga tgaaatggat cggtttctaa tgaacacgct
    30421 ggcatttagt gtgggattgg taacagaaga ttattccaca tttgatccag aagtattaaa
    30481 gataatggaa gaagaacctg attggctgca ggagtcagta gcgtggtgtc aatcgctagt
    30541 agtgggatca ttggtagata gtggcaacta tgatgataca ggtgagctaa tggatgaatt
    30601 taattgtctg cttaacctat atgatcgagc gagacaacga gagcttacat cgaatgagga
    30661 taatttgttt ttaaacatcc atgataagtt cttggctttg ctactaacag atgatgaatt
    30721 gataactaat ttattggagg tagaataatt atgcatatgg aaagtattag atacttcgat
    30781 ggtgagaaac acattgagta ttcaaacact caagaagatg tagactttat tagttttgag
    30841 gaagataaaa tcactaaagt taattttaaa gacggaacgt gcttgaagat agtttcgcct
    30901 tatattgaat acaaatcaaa atgggaacaa gatgattctg acgaacgaga tcgctttggt
    30961 tggtaatcta tggcagttaa aaaacaatgt aatcatgctg gatgtaaaac attgattgat
    31021 tataggcaga agtattgtgg gaagcataaa gcaaagcaaa cggcaattaa gcgtgaagaa
    31081 agaaagcaaa gcgaaggtaa atattttcaa ttctatcaaa gtagaacatg gcgaaaggct
    31141 tcatacttgt atcggttaaa tcatccagta tgtgaggatt gcctagaaga aggcttgata
    31201 aggaaagctg atgttgtaga tcacaaaatt gaactaaaag atgattggaa tagacggtta
    31261 gatgaaacta attttcgttc tttatgccat gcacaccata attccaagac agccaatgaa
    31321 agacaaagac gagaaaagag taccctttga gttaaggggg catagtgtga aacttactga
    31381 caatcgatgc ctactcattt tggtacaaat aaccgttgtg aaaaggcata agggtaatta
    31441 caaataacga ttacacttgt aatcacaagt gaaaaagtat ataatgaaag taggagattt
    31501 attgaaaaat acaattgtta ttactgataa aacagggaaa cagcgcacca ttgagttacc
    31561 tcattttggt caagtgacca ttcaaatgca gaacggaaaa atcatttata tagataaatt
    31621 agataaagaa aaattctgat cgaaaaacga aggaatgtga gcttaattgc ttacgttcct
    31681 ttttcttttg tctgaaagga ggacaacatg ggacacccta aaatattaca cgatacaaaa
    31741 ggcaatatat caagtgaaga aaaagcaatc cgtgtggacg ctagagaaga attgttcaag
    31801 cagcaaccat taataaatat cacgccccct gactggatgg cagcgagcgc tagaagtgaa
    31861 tggaatcgta tcgtaccaac attaaaaaaa gattatccat tgagtgaagc agattatggt
    31921 tcattggtag cctattgttt agcctttgct cgaatgaaaa cagccgaagc cgagataaga
    31981 aaatcaggta cgtttatcac atgcgaaaac ggagtaaaga aagccaatcc agcggttcgg
    32041 gttcaatcac aagctatgag tgatttgaaa aaacaagcca cctcactagg tatgacctta
    32101 gaatcacgat caaaactagc tttgaacaag gttaaaaatg atgaacccga agacccgttc
    32161 aaagagttga tgaattcatg aatgattaca ttgagaaagt cctatcaggt aagctgattg
    32221 cacccaagaa aattatccaa gcgtgtgagc gtcatataag cgatttggag cgttctaagt
    32281 cagatagctt cccttatgtg ttcgatgaag aacaagccac caaagcgatt aaattcattg
    32341 agttgctacc atctacggac ggtaaagcaa tcaagatgtt aggatttcaa aaattcattc
    32401 taggcagtct ttatggctgg cgtactaaag aagggaatta caggcgattc aatcgagcgt
    32461 ttaccagtat gagccgtaag aatgggaaaa cgtatatcgc aagtggcatg gctgccaatg
    32521 cgttgattat ggaacaagaa ccagcagaag caaggcaagt attgtttgta agtaacgctt
    32581 tgaaacaagc taaattgggc tatgatatgc tgtctaattc acttagaaac gtggtcaagt
    32641 ctagtaagtt tttaagacca caactgaaaa ttatgaactc taagattcag cacttgccct
    32701 ctaattcgtt cgctatggca ctggctagtg aaaccagcac gctagatggg tttgcaccaa
    32761 caaccgcaat tcttgacgag tggcacgaag caaaaactcg taaaacgtac aacgtcatta
    32821 agtcaggaat gacccaacaa aagaacggct tattgtgtgt tattagtacc gctgggcttg
    32881 atttaaacgt tcctatgtac gaggaatact tattgttaga tcgtgtgcta aaaggcgaag
    32941 aacaagccga caggtacttt atagcgatat gggaattgga cgatcccgaa gaaattcacg
    33001 atcaagagaa atggatcaaa gccaatccga tttttgaaag tgaagaaatc aaaaaagtaa
    33061 tgattccaac cattcaagat gatgtgaacc ttgctttgaa acaaaataac cttaattctg
    33121 tattggtgaa aaacttcaat ttatggagac aagcgagtga ggacagctat atgattgctg
    33181 aagactggca ggcaaccgaa gtagaaccac aagatattac aggcaagccc gtttatatcg
    33241 gagtggattt atctaaaaca gatgatttaa ctagcgtttc atggatcgta ccgctagata
    33301 acggcgaact ttattgtgat tcacatagct ttgtagccac caaatatggg cttcaagaca
    33361 aagaaaagcg cgacggtttg ccttatcgag aactagaaaa agctggtgag tgttccatta
    33421 ctcaattaga aagtggaatc gtggactatg accaagtatt tcagtttatt caagatttga
    33481 ttcaagaaaa tgatttggaa tgtatgggga tttgttatga cccgtataac gctaattcgc
    33541 ttatcagtaa agctgaaaaa gccaactacc caatgttaga agtaagacaa ggaacgatta
    33601 ctctaaacgt tccgacccga acttttagag aacaagttta tgaaggcaac gttattcaca
    33661 ataaaaatac gattctcacc catgcagtga acaacgctat tttaaaaacg gataacaacg
    33721 gcattcagat taataaatca aagaacagta acaaaattga tccaatagct gcattaatca
    33781 atgcctatgt gtttgcaatg gattacttca ccacaacgga aggagcgaaa gcagacaatg
    33841 aattttatac aagtgaagaa ttttctttct aattacattc ataccgttct tttacttctt
    33901 ggattggtgt gtgtgttggt tgctatcacc ttactaacaa atgtctacta tggcttgtta
    33961 gcgctgggca tagtgcttat tggggtagcg gtcatgctaa atacagaaca gaagggaggt
    34021 taaaagatgg cattttttaa agcgagacaa aatacaacgg gagatccttt cctacaacat
    34081 gtggtatcaa tccaatcgga tgattacacc accagcttta caagcgttcg tgcattaaga
    34141 aatagtgatg tgtttgcagc cgttcgaatt attgctagtg atattgcttc aagtccgatt
    34201 caattggtca aaaacaatat gccacaagct gataatgaac tggtgaagtt actaaacgag
    34261 aaacctaatt cagaaatgga tggttggcat ttaaagtttg ctttggcggt caatatgttg
    34321 ctaaacggta atagctttgc agagattaaa cgcaacggtg aaaaggtaga agaaattcac
    34381 ttattaccta actcaagtgt aacggttact caattagata atggtacgtt gtcttatcag
    34441 attggtgata aaaaaagacg tgtgaagtct agcgatattt tgcacttcaa atatttcacc
    34501 caagatggtt taacaggatt accaccactt tatgcgttac gtgatgaact gaaaattcaa
    34561 caagctggca accgcacatt gcacaatttc tttactcgtg gtgtcagtgg gtcaggtatt
    34621 ttgaaggttc ataagtcaga tttagacgga tcagcaaaga acgcaattcg tgaaaagttt
    34681 gaagaagcca acggttcaag tagtggagat aatgctctta gaacgatcat tcttgatgaa
    34741 acaatggact acaaaacatt agaaataaac accgatgttt tgaagcttat caattccaat
    34801 gattggaaca caaaacaaat tgccaaggcg tttggtgtac cgattgagcg tttaggcgtt
    34861 gaaaatgaac attctagcac cgttcaaagt aatctccagt atatccaaag tacgctgatt
    34921 cattacttta atgtgtttgt gagtgaatta gatacgaaac ttgaaaccaa tatccgcttt
    34981 aattccgatc agttactaga gactgatcct gaaacaaaag taaaaaatgt attggatcag
    35041 gtcaaagggt cacttctcac gattaatgag gggcggtcga aaatggggct accccccatg
    35101 gatggtggcg atcgtttact agcaagtttg aactttacgt atttagatac gttggagaaa
    35161 tatcaattaa aagaacagga aggagttaca ccagttgaat aatgaagaag aaaaggaaaa
    35221 acggctgaca gaagaagctg agctaacagc cgattctccc aaagtagaga aagaaaatga
    35281 aaaacaacca acagacggca aaactatttc aggctatgcg ttgaaattcg ggcaaccgtc
    35341 aaaagattta ggcggctttg tagaagtgat tacacccgaa gcattaaaag aggtggattt
    35401 atcaaatgtg ttcttattat atggacacga ttattcaaag ccactagcta gtgtcaaagc
    35461 aggtacgttg aaattgaatg tcgatgatac aggattacat tttgaagcag agctaaccga
    35521 cacgacctat tcaaatgatg tgtatgagaa tatctcaaaa ggggttgttg attctatgtc
    35581 ctttgggttc gtgcttggat tagattcatt cgacaaaaaa gaagatggca caattgaacg
    35641 atcaatagat aaaatcaaag cacttaatga aattagtgtc gtgaccgttc ccgcttatga
    35701 ttcatcaaat gtccaagtaa ataagcgttc ttacgaatcg tttatgagta acaaccaagc
    35761 aaaacaaaca aacaatagct tagaatccac ttctaaagca caaaaggaga gtaataacat
    35821 ggaaaaaact ttaatcgata acgaaaaaac agaattgcgt gggtatgaag aatatatccg
    35881 ttcacaaggc gaagtgcgtg atggagtcac tactgtaaat gcagcggcag ttgttcccga
    35941 agaagtaatc ggtgaagtct ttgatttgaa acgttcaaat tataacttag ctcaatatgc
    36001 aacagtaaaa acagtatcaa atggacaagg taaatatcca gtagcaacta accaacaagc
    36061 agtgttagca acaaaagctg aacttgctga aattggtgat attgacgctg aaatgtttac
    36121 ttcagttgat tataaagtag aaactcgtgc tggtaagatt gccttatcaa atgaggttgt
    36181 ggaagattca gcagtgaata ttgtacaaga ggtcaaagat cagttagcga aattggtaga
    36241 aaacactgac aataagcata tcatggattt attaaaaaca ttcactaaga aaacggctgc
    36301 tacgttggac gatttgaaac aactatacaa tgtggcatta gacccagcat taaataaaat
    36361 ggtaattcta aaccaaagcg gatattacca cctagataca ttgaaagatt cagatggacg
    36421 ttacatttta caacccgatg tgacagcacc tagtggtaaa tcattattcg gtatgccagt
    36481 agtattgatt gcagatacat tgtttgccaa tcctaaagct ggaacattcc ctatgattat
    36541 gggggatatt gcacaatcta tctttgttgc tcgtagaaat caagtaacga ctcaatggga
    36601 aaaattcgat tactactcac aaggacttgc agtgatcgtt cgcaacgatt ataagaaaat
    36661 tgatcaaaat gcttcagtgt atattgagtt tacgccagtt gtaacaccaa aagtatagaa
    36721 aaatattggg cggcggttta tcccactgcc tttttttcat taggagagat aaaatggtaa
    36781 ttttggatag cataaaaaaa agtatgcgga tcgatcacac tattgatgat gactttattc
    36841 aacaattgat tgatacagca ggcgaatata taaaaagtgc tattgatagt agcgcaacgg
    36901 ataaagatat ggataattat cagcaatttg atttggcggt gtcattactt actcaacatt
    36961 ggtatttgaa ccgtcaagaa gccagcagcg aacggatacc agtaacggta caagcattag
    37021 tacaacaaat gagaggtgct tattatgcca atcattaaga atgtaaatga attgactgag
    37081 agagtcaagt ttaaaaagac aaaacgggta aaagatgaag atgggcagat ggtggatagt
    37141 gaagaaactg tatttgaatg ttgggctaat gtgcgttcac aaatgctaaa agacgtgctt
    37201 gctagtgtag gtaccattct tgaaggaacg ttgacgttta ttattcgata cgatcaagat
    37261 tatgaactaa caaacgatat gaaagtagct tggaaaaaca agagttataa aattatttca
    37321 atcaatgaag gaacggcgtt taaagattat acaacgatca tagctaaaca gatttcttag
    37381 aatgattaca cttgtaaacg ttgtgggtat ttagtataat taaggtagta aatgaagggt
    37441 tagctgcctg aattttgcaa gacttagcta gtcgaaatct attgttggac tgaaaattgt
    37501 aggtgtggtt gcaaacatgc tggactaaga aagatgatag atgatgaacg ttcaatttta
    37561 atacaggcat gctaggactg attggatggt tttggcaagc tgattgtgta gaaatcaaag
    37621 aacacgtctg ttgtggacgt gttttttttg ttataattca aaagaggtga tttaatggac
    37681 aaagcagagt ttacaaattt ttattggaag tattatctac atttagaaaa tgagtttatt
    37741 aataccacag attttgtgat gctcgatgag aataatttca aaacattctc tattgaatat
    37801 caaaaactac tactagcgat tggttcggag tgtgagatta ttttcaaaga actttgcgga
    37861 tttgatagca gtagcaataa aagcataact gattttaaag ctgttattaa atctagcgaa
    37921 ttaatgtgtt tggataatgg tgttagggtt atgaattctt taacattgac ttcactaaaa
    37981 ccttttggag atgggtggcc tgaagataca ccagattggt ggaaaatata taacagagta
    38041 aaacatggtc gttcttcaaa ttataaggaa gcaaatttgg aaaatgtgtt gtatgcttta
    38101 gcaagtctat atttattaga ggaatatttg cataagaaag taatactaga aggtgaagta
    38161 gactttataa gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg
    38221 atgcaaaaag tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt
    38281 aaagaacaag aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt
    38341 gtatgccatt tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt
    38401 cataaacgct gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg
    38461 aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa
    38521 acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat
    38581 tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag
    38641 gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt
    38701 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact
    38761 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
    38821 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
    38881 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
    38941 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
    39001 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
    39061 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
    39121 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
    39181 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
    39241 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
    39301 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
    39361 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
    39421 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta tactatgata
    39901 aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac gggaaatgcg
    39961 tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt atgatatacc
    40021 attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga aaaatttttt
    40081 cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta
    40141 tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt ctaaatatgc
    40201 tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagaag cgaaaaaacg
    40261 aggaataacg acaataaggc ttaattctgc agaatacaga gtagaagcac accaatttta
    40321 tgaacacatc ggttatcatt ccgataaaat gcaaaaaaga tttttgaaaa tcctgtaaat
    40381 ctttatcaaa aaaatacccg atcaccaagc cgatacttga tgatcaggta tttttgtttt
    40441 tttgactaag aaagtaattg catcaagaaa ccagagatga tcaaaacgat ggcaccacca
    40501 agtcgattgc ccatttgagc aaaagcaata agttccatcc gattagctgc agataagacg
    40561 gctacatttc ctgttccgcc catcgagttg ttgcataagc cagcagtaat agttgattca
    40621 attggatata aaccaaacaa acgaccgaca aatcctgaaa caactgaaat cgcgataaca
    40681 cttgtcagac ataagacaac aaattgccat gtcaaagcag aagctaatac attcagattc
    40741 agcaacgcaa taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagtta
    40801 ttgaacatga tagctgcatc ttcataatac tctggtaata ggttggcgat tttacagatg
    40861 acaacgataa taatcataaa tgcgtaagca tggactttag gaacaaaata gttaccaatt
    40921 tgtcctaaga tgaagaaagt caatgatacc atcaaaccaa cacctaactg tggaatggaa
    40981 ggtttcacag ttcgaggttt tccatcgcca agatcgcctt tttccattaa tttaccattg
    41041 ccgttgaatt ttggcattga ttgtccagca cgagcaacta atgctgcacc gatgatagct
    41101 aagacatttg tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcatccgta
    41161 cctaaaacat ttgcataaat cgttgataaa ggaacggctc cggctccaac accaccagcc
    41221 atcgcaggta atgagatgta taaaacggaa tttgcaaaac catttccgat aagcatgccc
    41281 acgagtccta cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga
    41341 acagaagctt tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca
    41401 gcaatataaa agtcccaaaa gcccatattg ctgacaaagt tttctgtgga tttcactaca
    41461 tattcaggta agatcccaaa tgtagcaatc gcagcagaag cgaagatcgc aaaaacggaa
    41521 ccaccaccca aataactttt gacgataggg atttttgtcc cgataaaatg aaataaatta
    41581 ccgaaaataa ccaaaactgc gattggtcca atcatattcg tcggtaattt ccctaaagcc
    41641 attgcaatga tcaggacaag gaccatgatc aaataaagtg gcaaacttac gccggtgatt
    41701 tttgtggacc aaaaaccttc ttttttcttt tcctcaactg cagttgcagt tgttgttttt
    41761 tgtgccattt ttacagatcc tttcttattc tgtatcatat gaaaaaagcc aaagaaaagc
    41821 gacaaaagag caggagttgg ctggccgctg ttttgccgca gtcgcttttt tctttttaga
    41881 cgtcatacaa agtttctgta tgttctttta ctataacgat cttaatcttc taaagattta
    41941 tattcaggaa cccatttagc agattcaact gcttctttga tatccgtgat ttcttcacga
    42001 ttcaagtttt gtgcgacaac tgattttgcg acagtttcag caatcgtttg agaaaattca
    42061 gtgatttttg ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg
    42121 cctaatgctc gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca
    42181 atcaaaccaa gtcctaaacc tgggtacatc aaagcattat ttgcttgacc gatttggtaa
    42241 gttacaccat tatattcaac atcagcagca gggatacctg ttccaactaa tgcttttccg
    42301 tccgtccatt ctattaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa
    42361 ggcatgatga tcggacgtgg tgtatgagca gccatttctt tcacgatttc ttcagtgaat
    42421 gctccaggtt gggtagaagt accaatcatg attgtcggat ggatcgcttt tacaactgct
    42481 tctaagtttg tcaattcatc agcattagtg aaatctgagc gtttgcgagc gaatgggatt
    42541 tgaccaggag tcaatccttc tgtatcttca aacaataaac cttgtttatc tacttgatag
    42601 aaatgttggc gtgcttcttc ttcaggtaca ccttgacgga tcatctcatc taaaaggatg
    42661 tttgcgatac ccacaccagc agtaccagca ccaaatgtca aaattatttg atctttcaat
    42721 tgttcaccgg atatattcaa tccgcctaat acacctgcta ggacaacaat tcctgtacct
    42781 tggatatcgt cgttgaaagt agtgatttga tcttcgtatt tttctaagat cgctgcagca
    42841 tttcctcgtc caaaatcttc ccaatgcaat aacaattctg ggaataattc ggtagctgat
    42901 tgaacgaatt gatcgatgaa ttcatagtaa gtttcgcctt ctacacgagc atgacgattg
    42961 cctaagtata agggattatt caataattct tcattattag taccagcgtc gatcgataca
    43021 ggcaaaactt gtgctggatt aataccagca gcagcagtat agaccatcaa tttgccgata
    43081 gcgatgtcaa caccgttcac accccagtcg cccataccta agattccttc tgcatcagtc
    43141 acaacaatca aacgaatgtc tcgtccatct gccgcgttct tcaaacttgc tttgatgttt
    43201 tctggttcgt cgattgaaag aaatgcagca tcttgcggtt tcaagaaaat ttcgttgtat
    43261 tgttcgattg aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt
    43321 tcgcccatca atttataaaa caatgtccga ttcgtattga atagattcat taagaagata
    43381 cgtttttcca aatctgtttg tttgcttaag tattgaccat aagcttgaac agattgttgt
    43441 tccagtgttt ggacagtact tggaagcata ccagtgattc catattttgc ccgttcttct
    43501 tttgtaaaag ctgttccttt atttagaaaa ggattattta ataagtttaa ccctgtaagc
    43561 atgaatgatt cctcctaagt tttaatacaa acgaagtata gaagtcagat gatgatatag
    43621 tcaaaaagag caggtatgat aaattttatt tatatgtaat atagccaaaa gaaatcaaaa
    43681 tagcagtatt tgttcttttt atatcgacaa tccttgcttg aatactatgc gtatctctta
    43741 tatgctagag aaagaaaagt agaagaataa gggatagatg gaatggataa ataggatttc
    43801 tgaggaaaaa agcacatgat agcgttgata ggagtcacct atgtcaggtg ataaaaatac
    43861 ataagtagaa aattaagagg aggaagagaa aatgaaagaa gttcaattga ttagaaaaag
    43921 cgaactatca gaaggcggtt gcaatgcatg tggcgtagta gaagcgacaa gctatacatt
    43981 aaaattagat tccaatcaag caataatctc tgaactgaca gtaggcggtc ttgtggacag
    44041 tctggcgctt gctgaaggtt ttactggaga agatatttat gagatgtttt cagaagtccg
    44101 tcagttaaaa aaaggagaaa aatgcataga ggttcatcat gagagcccga atgtgaggtt
    44161 caaacgtggt gataatgaga tgatcttcaa gaatcatgtg tctgatcaca ctgaactata
    44221 tgaaatcgtg aatcaaatac tgtctgggat ttttgaatta ggaccttatg agttcaagga
    44281 agaaaacggt aatccaaaat taaatgaaga atggcaagaa acgattgaaa cacaaagaaa
    44341 caatccacac ttatttcaat agtcttccac agtatttcgt aaaaatgttg atgaaaaaga
    44401 aacaccgtga atcagaggtc gtgaaaaacc tgccctgcat caagtaataa gaacagccaa
    44461 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagtttt
    44521 tgaacaccgt ttaaggaaga gactgtgaag tcaacgttct gacctgagta ttagagagac
    44581 aaatggcaaa atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg
    44641 attgaacacc gttttatcga gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    44701 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac cgagaaaatg
    44761 gcttttgaac accgtttatt caaaaaaagg gactttgaca ctacttttgt caaagtccca
    44821 tcggtttttt atctattttt attacaatca tttcttccag agtagtgaca tctggaatgg
    44881 ctgaaatgta aacagattat ttgcctgtat caatgtcgaa gagtaatata aaaagtgtaa
    44941 acgctatcat attgttcatc attcaaattt tatagagaga aggcattgag atggaaaaaa
    45001 catatcaagc aggtacaaat gaaggaatag tagattttat caatatggaa gatttagaaa
    45061 tcgcggcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
    45121 acttatttac ttatcaagaa aatgaacggg cattcaatca tcgtttgatt attcctcatg
    45181 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacgaagaa atgctgactg
    45241 caccaatcat catggcacca gttgctgctc atggattagc tcatgtgaag gcggagaaag
    45301 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
    45361 gtactttaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
    45421 atatgagcaa agataatgga atcaatttgg atattcttga agtggccaag cgtaatggag
    45481 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
    45541 gaaacgggtt taccttcccg ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
    45601 gccaaacgat ggatgctgta tataaatctt ccaaacaaaa acttagtcca aaagatgtag
    45661 aatttatcgc tgctcattct gatttgccag tctacgtgaa aggtgttcaa tcagaagaag
    45721 atgtctatcg atcattagaa tctggtgctg gtggaatttg ggtatctaac catggtggcc
    45781 gtcaattaga tggggggcct gctgcgtttg attcgttgca atatgtggca gaagcagtag
    45841 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg tggacaacat gttttcaaag
    45901 caatcgcatc aggtgccgat cttgttgcta ttggcagacc cgttatttat ggattgtctt
    45961 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
    46021 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
    46081 atcgttttat ttaagaaata aatgacgaaa aaaataggag tgcggcagat gatgccgtac
    46141 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
    46201 tgaaatattt tttcatactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    46261 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    46321 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    46381 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    46441 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    46501 aataccgttg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    46561 gaaaatccag caactggcca gacaggttct ttaacattgg atgtagttcc tgaattgcca
    46621 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    46681 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    46741 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    46801 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    46861 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    46921 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    46981 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    47041 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    47101 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    47161 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    47221 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    47281 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    47341 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    47401 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    47461 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    47521 cacatgcaga aacaactata cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    47581 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    47641 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    47701 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    47761 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    47821 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgaatttt
    47881 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    47941 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    48001 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    48061 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    48121 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    48181 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    48241 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    48301 tatgatctaa gtccagttcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    48361 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    48421 gaaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    48481 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    48541 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    48601 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    48661 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    48721 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    48781 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    48841 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    48901 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    48961 tgccggtgcc cccatcaagg ttactagcgg tagtttatta tcagcaggaa gccatacctt
    49021 gcgttttggg atggggtcgt gggatacaaa tactctattg gcagatatta ttgctattat
    49081 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    49141 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    49201 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    49261 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    49321 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    49381 agagataaat acgtggaata gttacatctc aacaagtgat tcaacaggaa aatataatac
    49441 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    49501 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    49561 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    49621 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    49681 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    49741 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    49801 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    49861 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    49921 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    49981 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    50041 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    50101 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    50161 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    50221 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    50281 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    50341 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    50401 cattcatctt acttacaact atcaattaaa tactgtagat acttctgtac aatccgtcag
    50461 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    50521 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    50581 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    50641 aagttattat gaagcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    50701 cacgtctcct tggcaactga cagggacatt gacttcttta tttaagaatg atcaaggtca
    50761 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaaacaat tgattcagca
    50821 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    50881 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    50941 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    51001 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    51061 aacttctgct gttaaaatca gaaatgcttg gaggagtgga cttggaactt ttcagtttgc
    51121 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    51181 agatttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    51241 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    51301 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    51361 gctacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    51421 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    51481 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    51541 ccggtccaga aagtcaaatt tgttattttt atttccaatt attttggttt ggacgtcctt
    51601 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    51661 aaaaagagtt tagttttact attacagaag aaggacgatt gaaatttgaa atatggcttt
    51721 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    51781 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    51841 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    51901 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    51961 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    52021 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    52081 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    52141 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    52201 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    52261 ttgaattatt tgctcgctat gcatctattt tgcggacgct gatcaagcag ttagaattac
    52321 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    52381 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    52441 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    52501 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    52561 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    52621 cattggtgct tcccatgcag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    52681 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    52741 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    52801 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    52861 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    52921 taaattaatc gtagcaacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    52981 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    53041 agcaaaagaa gtagtgatct tcggtgctgg ttatattgga ttggaattag cagatgcttt
    53101 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt taacaagata
    53161 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaatcaattt
    53221 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    53281 acttctatct caagcaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    53341 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    53401 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    53461 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    53521 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    53581 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    53641 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    53701 tgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    53761 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    53821 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    53881 acacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    53941 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    54001 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    54061 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    54121 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    54181 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    54241 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    54301 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    54361 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccagacacc tggagtcgtg
    54421 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    54481 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    54541 gtaggcgata ttagtttgaa agaagaatat ggtctagccg taggagatac agttaagtta
    54601 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    54661 tcaccagttc tctatacaac catcaatgct taccaagaga tccgcttcga aaaagaagat
    54721 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    54781 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    54841 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    54901 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    54961 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    55021 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    55081 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgctggta
    55141 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    55201 gcaattggat aagaaggagg aagagcatga aagcaataga atttaaagaa gtagaaaaaa
    55261 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    55321 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    55381 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    55441 gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    55501 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    55561 tcaaacatga acatcgtcaa aaagaagcaa aagaattatt tgagcagctt ggcgtggata
    55621 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    55681 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    55741 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    55801 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    55861 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    55921 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    55981 tccaagggat atctcggcac ttcaacaaga gagatcgcac aaaaagttgg gatcacccaa
    56041 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    56101 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    56161 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    56221 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    56281 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    56341 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    56401 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    56461 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaaatagtt
    56521 aggtgaagaa gcaagatatg acgaagatcg ttatatcttg ttttttttat ataggtagtt
    56581 tttcataaat acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat
    56641 gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga
    56701 aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata
    56761 tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta
    56821 cagccccttt tttgcgtact taagctgatt ttaagttttg acccttagtc tggatctacg
    56881 aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt
    56941 attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc
    57001 agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca
    57061 ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca
    57121 cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat
    57181 cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc
    57241 acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct
    57301 gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat
    57361 ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt
    57421 tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa
    57481 gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa
    57541 gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt
    57601 gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa
    57661 gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat
    57721 agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca
    57781 caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc
    57841 atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca ccgtttaagg
    57901 aagaggttgt gagcctgccg tttaactccg cgtcacaagg agcaatttca aaaaacagct
    57961 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta
    58021 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagcagctt
    58081 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    58141 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgatggaag
    58201 ggtgattcca gcttcagctg atgctttgtg taactcttcc aaaaagctgg cacgactgat
    58261 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    58321 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    58381 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    58441 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    58501 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    58561 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    58621 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac caaaatagct
    58681 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    58741 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    58801 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    58861 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    58921 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    58981 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    59041 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    59101 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    59161 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc
    59221 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    59281 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    59341 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc
    59401 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    59461 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    59521 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    59581 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    59641 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    59701 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    59761 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    59821 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    59881 gatcaggtat ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    59941 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    60001 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    60061 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc
    60121 caaaatcaat tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt
    60181 accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg
    60241 aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg
    60301 gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa
    60361 acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga
    60421 aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt
    60481 caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa
    60541 cttgaaaata tccgtcccca tatgaaacgg atcgagatcg gagcagactt gaatggagtg
    60601 gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat
    60661 gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc
    60721 acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact
    60781 gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct
    60841 gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg
    60901 atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta
    60961 tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat tgaaccaaaa
    61021 caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt
    61081 caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac
    61141 aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa
    61201 gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga
    61261 catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat
    61321 caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta
    61381 ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa
    61441 atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt
    61501 ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa
    61561 aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct
    61621 ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg
    61681 cattatcctg aattgaaagt ttatggatac aatcgggcaa atacgattaa tgccagcatg
    61741 gagttcgatg tggatacact aagtccaacg tatcgtttgc tgattggcgt tcctggacga
    61801 agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg
    61861 aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat
    61921 cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa
    61981 gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa
    62041 atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga aaaagcagaa
    62101 aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag
    62161 catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag
    62221 aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg
    62281 ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa
    62341 gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct
    62401 caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg
    62461 ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa
    62521 tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga
    62581 acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc
    62641 tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa
    62701 agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta tactaatcat
    62761 agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg
    62821 aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc
    62881 gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa
    62941 ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca
    63001 ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt
    63061 caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa
    63121 gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta
    63181 ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga
    63241 catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa
    63301 aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga
    63361 acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact
    63421 ggcagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc
    63481 gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat
    63541 cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat
    63601 gaaaaatatc cccgtttggt gattactcgt aaagtactaa aagacaaagc actgtatttt
    63661 ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt
    63721 cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga
    63781 caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat
    63841 gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa
    63901 atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc
    63961 cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga
    64021 gatgtatttg gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt tttcgtaaga
    64081 caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa
    64141 gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa
    64201 gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg
    64261 ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa gaatgcctca
    64321 gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca
    64381 gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat
    64441 aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa
    64501 ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat
    64561 gcatcaatga gagaagtcat ctatcgcaga tattcacgag tgattcgcga agggttgcct
    64621 ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc
    64681 ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa cgataaacat
    64741 aaaacaaatg agttgctatt cggtatagat ttggctgtag taccgcttga gcgaaattca
    64801 ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc
    64861 catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta
    64921 ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca
    64981 gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt
    65041 gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca
    65101 ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc
    65161 tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag
    65221 gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc
    65281 gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt
    65341 ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt
    65401 ggggagaaag attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa
    65461 gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag
    65521 attgaaccaa aaatcgattt ttggatggaa aaatttcagg taaaagggaa aaagacggat
    65581 aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc
    65641 catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct
    65701 gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct
    65761 agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga
    65821 aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta
    65881 ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg
    65941 atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa
    66001 atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact
    66061 ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac
    66121 caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc
    66181 ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt
    66241 caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa
    66301 aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc
    66361 ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat
    66421 gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac
    66481 tggcttgcaa tcgatgcttc gagcaagtca attaggcatt tcaccagaag aagtagctag
    66541 cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat
    66601 gacagttgga gaagataact cgacagtcca atatgtcgta agttatgtag caacgattgt
    66661 cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa
    66721 aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg
    66781 aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt
    66841 tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat
    66901 ctcattggat aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg
    66961 cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga
    67021 tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggatata tgcttggatt
    67081 gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt
    67141 cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta tgggtggagc
    67201 attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa
    67261 catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt cattgaaaca
    67321 atcgatttca attatgagaa atgaacgaaa aaaagcataa aatatagaaa aatatagaag
    67381 gaagctggac atttattgtt cccagcttct tttttgtata gacctatttc tattttttta
    67441 ttatattgga tatatatatt cattgaaaat agagcataac aactttataa tgttgagtat
    67501 accaattttt ggaggcgttt taatggaccg ctatttagat ttactttcag agaagttccc
    67561 tacaacggaa gatgtcatca cggaaatcat caatctcgaa gcaattttgc agcttccaaa
    67621 aggaacagag ttatttttga gcgatataca tggcgaattt cctgcttttg atcatattct
    67681 gcgtatcggt tcaggaaatc ttaaagaaaa gatcaaagac cttttctctg aacaaatgac
    67741 ggaaacggag atcagccaat tcactatttt tacggcttat ccagaatatg tgttgacgac
    67801 tgattggtac aaaaaacaag ataaatccca gctgatccat cgtttgatcg atcttttacg
    67861 gtttacgaca gtcaaataca ctcgatcaaa agttcgaaaa ggattgccga aagaatatag
    67921 ctatatcatt gaggaactct tgtatatcga tgatcgtatc aacgggaaaa aagactatgt
    67981 aaaaaaaatc attgaacaac ttgtcttgat gaaggaagaa gaaaaatttt taacaaaact
    68041 tgcgcagacg atccaaaaat ctgtcatcga tcatttgcat atcgtgggtg atatttttga
    68101 tcgaggaact caagcagcca aagtaatgga tcgaatcatg gatttttcct ctgtagatat
    68161 ccagtggggg aatcatgata ttttgtggat tggtgcgtat tgcggctctg aggcatgttt
    68221 attgacgctt ttacggattg ccgcacgcta caactattta tttgaattag agaaagaata
    68281 tggattaaat ttacgtccat tattttcttt tgcccatcaa acctatcaga agaatccagt
    68341 atttacccct aaaaacagga aagatctatc tgaaagagag caagaagaat tagaaaagat
    68401 ccatcaagca ctttctatcc tccaattcaa atcagaacat cagttgttga atcgacgacc
    68461 tgaatttaaa atggacgatc gtaaattgtt ggaaaaaatc aactatgagg aatcaacgat
    68521 cgttttagaa ggtcagctct atgagttgaa agggacttgt tttcaaacga tccagccaga
    68581 tgatcctaaa aaattcttgg aagaggaaca aaaagtgatc gatagtttga tgtattcatt
    68641 ccagcactcg ttaagaatgc aaaaacacat gacgtttttg atcgaaaaag ggggaatgta
    68701 tctcacgaac aatcagcatg tactgttcca tggctgtata cctgtagatg aaaaggggca
    68761 gctcttagca tttgaattag accaatctta tcgcggaaaa gaactgctgg gattttttga
    68821 aaaacagata actgaaagcg caaaagattt gactgcaaat gaagatttgg cgaccgattt
    68881 gatttggtat gcgtggagcg ggccattttc gcctttattc ggcaagagta aaatggcaac
    68941 ttttgagcgc tattttctca cagagcctca tacgcacgtc gaaaaaagca atccctatta
    69001 tcacttgcga gatgaagagt ggttcagtga aaaaattttg gcagaattcg atgcaaaaga
    69061 agaaggttca gcaatcatca atggacacac gccagtaaaa gtaaaaaaag gagagtcacc
    69121 gatcaaagca aacggaaaag catttgttat tgacggaggt ctatccaaag cttatcaaaa
    69181 aacaaccggg attgctggct actcattact atgcaattct tacggtttcc aaatcgtgac
    69241 acattatccg ttcttgccta ttgaacaaca atttgcaaaa aaatctgatc aaacgaatgt
    69301 aaaaaaagtg atcgagcagc agctcccacg caaacttaat cggaatacga caaaaggaat
    69361 ggaattacag caacagatta ctcaactcca aaggctgttg gattatcgaa aggacgattg
    69421 agaaaaatgc tagccaacat tgctcaatag actaaaaagc gactgcaaca aaaggcgtgt
    69481 cgcagtctcc ttcggaatta gctcaaaaaa cggtaaaata ttagaagcta tttttctgtt
    69541 tatttctcta atactcaggt cagggcgctg atttcacgac ctcttcctta aacggtgttc
    69601 tcctgccaac tcctcggtca caagtcacaa tatccaacaa acatgagaag ctgttttttg
    69661 aaattgctcc ttgtgactca gagttgaacg gcaggctcac gacctaatca gaaaaacgat
    69721 taaaattcat tcacaaagaa atcgaataaa cgctgttcag atgaatctac acgatgagaa
    69781 agttccggat gccattggac accaaggatt ttgtgcatat tatctttact ttggactgct
    69841 tcaatgagtc catcgttgga aaaggcgatg gcttttaaca aaggagaaag atctttgact
    69901 gcctgatgat gataggaatt cacaaagtat gaatcaggga gaaggtcact taataaactg
    69961 tccgaaacga ttttgacttc gtgagttgca aactgcgggg cagtgggctg ttgttcgtgt
    70021 tttacggacc attttggata taatgaaaga tcttgataaa gtgttccctc caacacgaca
    70081 ttcaatagtt gaagtcctcg gcagactgca aaaatcggtt ttctttgttt caatgcttcc
    70141 aagatcaatg cctgttcgaa ttggtctcgt tgaatatttg tttccgtcag ttttggatga
    70201 gggtcttcca tgtaaagttg cggactaaca tcctgaccgc ctgcaagaat gagtttgtcg
    70261 attttctcaa ttagttgagg cgctgaatct ggagacatga ctggcagaat aagaggtaat
    70321 ccttgagcat ctaaaactgc atccacaaat ccctgcggtg tatagcttac ttgatttcct
    70381 tggaaagtat ctgtcgctcg aatcagttga ttcccggcaa tgccgatgat tggtttcatt
    70441 tttattcccc ctaaagtaga ctaattgtaa caaggattta aaaaaattta aactatgtaa
    70501 gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag aattttcgtg
    70561 tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa agtaagcaga
    70621 aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga gaaggacatt
    70681 ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt caatgaaaaa
    70741 agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta gaagctttgg
    70801 aacaaatccg aaaagcagaa gaagaaaatg agaagcgtgc tcgaacccta cggcagcagt
    70861 tgaagaccta tgcagaagaa aaagagcaag aattggagat aatccaaacg aacagtcggc
    70921 aacagctgaa acagttggta gaagaaaaag aacaattcga aaagcaagaa ttagaaaaag
    70981 aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa gaccaatatc
    71041 aaaagcatca gagtcaagca atcggtgcaa tcattgaaag ggtgaaagaa acttatggcc
    71101 gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga gaggatttta
    71161 caagcagtac aaggaatcca aaatgttgaa atccgggatc tgtatcaaaa tagaacaaac
    71221 aaccagtggg tagaaaaata ttttccagat accacgatta ttgataaaga agcaaaatta
    71281 agcgctttag gcaatcggct gaaagacatc caagaagcta tccaattcat acaacaccac
    71341 ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt acaagacttg
    71401 gaagcaaatt attccgaaga aaatttcatg aataaattag aagaagtcct tgatttaaaa
    71461 gaaaagtggg aacagctaac tgaaaaacgt gatcgtttag aagaagagga agaatggctt
    71521 cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac gaccattttt
    71581 gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact aagtaagctt
    71641 gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct ttcgtatgtt
    71701 gctttaaaaa aatcagttgc tgctatagaa gaaattgctg ggaaatacgg gttcaataaa
    71761 gaaacgtatc cttatgaaca aaatcctaaa gaaaaattgc aagaaaccaa acagcaatta
    71821 tccattgttc acgagcagca aaaaaagata acgacagcgt taggtcagtg cagcggctat
    71881 atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg ggaaaaagta
    71941 aaagaacggt ttatccatgc cacttatctg atcgttttgc aaggttgggt agatgcagaa
    72001 gaaaaacaga atttgatgca tgtgttaaac gaaactgtat cagaagaaga tatatacgtt
    72061 agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa attgaaaaac
    72121 catccgctta tcgaaccttt tgaattgctg actgaaatgt acagtctgcc aaaatatgaa
    72181 gaagtagatc caacgccttg gatgacacca ttttatcttg tcttcttcgg gatgatggtc
    72241 gcagatgttg gttacggatt gctgatgctc attgggacaa tactagctca aaaactattt
    72301 gttttacccc gcgggatgaa acgtttcgta aaattctttg agattctctc ggtaccatcg
    72361 atcatctggg gattgatcta cagttcattt ttcggacagg cattaccaaa agaactattt
    72421 ggtatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac gattttgatt
    72481 ttatccgtga ttttcggtct gatacagatc ttggttggat tattcgtggc agcaaaggaa
    72541 aatatcagga ggaaagatta cttgagtgct gtcagtgatg gttttgcctg gcaagggatc
    72601 ctgattggga tcgttattgc attgattggt gtcatgttga taaaaaatac agcatttgtt
    72661 tatcttggcg cggggctatc gatcattgct gctttatgta tcttaatcgt gccgattatc
    72721 caatcgccgt ctaaagtcaa gggtgctgcc aaaggagcgt ataatcttta cggattgact
    72781 ggttatatcg gtgatttagt cagttataca cgtttgatgg cactaggtat ttcaggcgga
    72841 agtattggcg cggcattcaa tatgctagtt gctttcatgc cgccagccgc tcgattcagt
    72901 gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac actgttaagt
    72961 gcctatgtcc atggtgcacg attgcagtat gtcgaatttt ttggaaaatt ttatacagga
    73021 ggcggacgtg cctttgaccc gctgaaaacg gcggaaaaat acgtcaacat caatcataag
    73081 aaaaaaaaca ataaataatt ggaggatttt tatacaatga tggattactt aattagtcaa
    73141 aatggtggaa tggtatttgc agtattagcg atggcaacag cgacgatttt ttcagggatt
    73201 ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcgttaac aacaagtcaa
    73261 cccgaaaaat tcggacaggc tttgattctg caattgcttc caggtacgca agggttgtat
    73321 ggattcgtta tcgccttttt gatttttatc aacttaggaa atgatatgtc agttgtacaa
    73381 ggattgaact ttctaggcgc ttctctgccg attgctttta caggtttgtt ttcaggtatc
    73441 gcccaaggga aagttgcagc agcaggtatc caaattttag cgaaaaaacc tgaacatgct
    73501 acaaaaggaa tcatttttgc tgcgatggtt gaaacatatg cgattttagg tttcgttatt
    73561 tccttcctat tagtattgaa tgcctaagca taaaggagga aaagcagatg aacgccattg
    73621 aaaaaatcat ctctcagatg aatgaagcag cagagcaaga aagagcggcg ttagagcaag
    73681 aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaaatc gaaacagaac
    73741 atcaaaaaca aaaagaaaaa caaatcgaat tacttgaaaa aaaataccgt cagcttagga
    73801 atcgccaaca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa tttttacgac
    73861 gattgtttgc agatgcaata gctgaaatgg agaattggga tgaatctgaa caaatccagt
    73921 ttatcaaaaa ttcattgtat tcattacctt tgacaggcaa agtaacattc attgctggag
    73981 aaaaatcagc agcttatctt tctcagacat tgctggatga atggaacaat gaacttccct
    74041 ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg ataaacgatc
    74101 aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa ggaacgatga
    74161 gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg aataccacga
    74221 actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga catttgaacg
    74281 gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca cgatttacag
    74341 tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa aggagcagag
    74401 ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt ggatctatac
    74461 gatgcgctat actttccata atctgaaggt tttgacaaaa gccgagatga caaatcaaaa
    74521 tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga aggatgccat
    74581 tcatacgcaa gtttcaaatg aactccctga aagcattatg gacagtatac gagaagtacg
    74641 tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg atcgacagtt
    74701 tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc tgacagaagt
    74761 cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat tgcagaaaag
    74821 aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa aagaagtatt
    74881 tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag agacagatta
    74941 tgctgatttt ctgcgccctg tcatccgtga gggaaatctt gatctcgtaa agcttgaaca
    75001 gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg ctttcggacc
    75061 actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc ttcgtttact
    75121 ggttatcggc aaaagaagcc atttcgaacc agaaacaatc agaaagcggg tgagacaagt
    75181 ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca ttcaaattgt
    75241 ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca attgaagaaa
    75301 tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca ttgagcccag
    75361 aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt cccaatcatc
    75421 aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa gcagtaggtc
    75481 aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa atcataaaag
    75541 tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa gacatgtgtc
    75601 tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat gtggcgtcta
    75661 ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc tctactggtg
    75721 aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac ggaatccaac
    75781 ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc ggagttcaat
    75841 tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa gccggagaaa
    75901 cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata aaacataaaa
    75961 tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt tcattcacta
    76021 ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg acgatgcttc
    76081 aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg gatgcaccca
    76141 tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga ggagcagcag
    76201 ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt gcaaaatggg
    76261 ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag atgacggatg
    76321 ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg atggaacgta
    76381 cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct tctatctata
    76441 cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct atcatggcag
    76501 actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg gaagaaatgc
    76561 ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac tatgaacgat
    76621 ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc gcaatcagtg
    76681 ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca ttgcgggtcg
    76741 taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt ccttcgatcg
    76801 attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg gatcatatcc
    76861 ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag gaagaacagc
    76921 agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat gaccgtctga
    76981 cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct tttgatgatg
    77041 tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc ttgacctttg
    77101 gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc atggacggta
    77161 ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca gaattagaaa
    77221 aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt tcagaaggag
    77281 gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc attgatggcg
    77341 gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat gcaaaatggc
    77401 gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt ccaaatattc
    77461 gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg ccatccttta
    77521 gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg acaaccgaaa
    77581 gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga agtaatcaat
    77641 ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc aatcgatcat
    77701 ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg tcttcctcat
    77761 aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc agatgatttc
    77821 gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt catggaagat
    77881 tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc aaacgaccct
    77941 gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata tcttgcttat
    78001 gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc agaggcattg
    78061 cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc tgggtatctt
    78121 tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact aaaaggatcg
    78181 gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc aatccctgac
    78241 ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta caagagtggg
    78301 attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa gggaactgga
    78361 cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc agcctatgca
    78421 caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt atctgatgta
    78481 gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa tcaaggattc
    78541 tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct atcgatgttg
    78601 ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct gccagaaggg
    78661 aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt gactcgattg
    78721 aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa gcaagatgaa
    78781 ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca agaaatggaa
    78841 gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat cacggaacct
    78901 ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat cgtagacaaa
    78961 aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca gctgacagac
    79021 acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc aatcgatcgg
    79081 tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac ttgtcaactg
    79141 atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata tatgacgatc
    79201 cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa cgagcgcgca
    79261 gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt ggtatctttt
    79321 tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt ttgccggctg
    79381 ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc ctacttaccc
    79441 taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt ttcactgcaa
    79501 cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat tggaacagtg
    79561 ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt atgatggttc
    79621 cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt attatattaa
    79681 aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt tacatcctaa
    79741 aattggcctt tgttatccaa ttgattggta cggttgcttt atcttttgta tttgtgcctg
    79801 aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct agtttttgta
    79861 atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat gtttatttga
    79921 tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta tggcgtgatt
    79981 tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca ttaaccgtta
    80041 caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc aatggggcaa
    80101 cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg agtgtgacac
    80161 ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca ggtctgattt
    80221 taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc ggattgaaaa
    80281 caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga cgtacgagag
    80341 cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt actttatttt
    80401 ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact gaaacgatac
    80461 ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt ggtacagtag
    80521 gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc attatagcgc
    80581 ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg acaaaagcac
    80641 aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga taatttcatc
    80701 ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt tcttgcttct
    80761 tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt tgttgaacct
    80821 cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt tatttatcaa
    80881 taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca gaagctaaaa
    80941 tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc gcacgtgtag
    81001 taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc tcaattatct
    81061 tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga ttcgttggtt
    81121 tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga cttaatttag
    81181 tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact tccacatcta
    81241 atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt tggacatgta
    81301 ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc attccagcta
    81361 agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta tgttgatagt
    81421 aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct ttttcaatca
    81481 cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga ttattacaag
    81541 aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc aatatatcag
    81601 gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat aactgagcca
    81661 attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct ggagagaaaa
    81721 gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat agagattcta
    81781 aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat tgaaaatttg
    81841 tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta ctagttgatt
    81901 gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga ccaaaatcaa
    81961 tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca ttgggagaaa
    82021 taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca tatttataag
    82081 ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt tgggagggta
    82141 actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt tgaatcaacg
    82201 atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac gggtaattgt
    82261 cataagaatt aataggcatt ttctccactc cttaaaattg tgtaccatac aattttacca
    82321 ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct atgaaaatta
    82381 ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa gtaatgaaaa
    82441 aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg gtggaaattt
    82501 tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg ggggagaaca
    82561 gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat catttacctt
    82621 ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa gcattgataa
    82681 acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca acagattact
    82741 tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa gcattgtcgc
    82801 atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac gatattttag
    82861 aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa ataacgttta
    82921 taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt acgttgacct
    82981 ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat atcaatgctg
    83041 tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa acttctcaaa
    83101 tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt atagccatgg
    83161 cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta gtttctgaga
    83221 aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt ggtctagcta
    83281 ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg gctttaaaat
    83341 gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag gaaatccagc
    83401 agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa ttgccattga
    83461 aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg agcttagatg
    83521 gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa cagcatttgt
    83581 tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc aaagcggtaa
    83641 tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta ttatgccgaa
    83701 aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta aagaagccta
    83761 cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta aattgcagcc
    83821 agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga cagcagaaag
    83881 tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca atgaagatcc
    83941 agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa tgaataaaac
    84001 aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg aattaaaaga
    84061 agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct cgtttatttg
    84121 atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat agctacaatg
    84181 gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc tcattttaat
    84241 tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac tatcctcgga
    84301 tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc gcagtgccag
    84361 gtaatccttt taagtcagta atctcaagac cggcagcttt atgttgctcg tgtaattttt
    84421 ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca tacgcaccat
    84481 gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc aaataaacaa
    84541 gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca aatgcctttt
    84601 gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga gccattttca
    84661 tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa ggaaatagtt
    84721 gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata aaactgttaa
    84781 ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt gctgattatc
    84841 aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca gtaatcgagg
    84901 ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag acgaaagaag
    84961 aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt tcttcttatc
    85021 tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat tataatccag
    85081 gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa aagaccaata
    85141 aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt agccaccgat
    85201 gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt agatcagctt
    85261 tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc tacaccgtgc
    85321 tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac ggcatttccc
    85381 attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt atctccgata
    85441 gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac tgccgcacct
    85501 ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac cgttgtgtat
    85561 ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc tggttcgtcg
    85621 atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt tcgatatttt
    85681 agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc tcgattaggc
    85741 gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg tgccatcact
    85801 tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt acctgatttc
    85861 gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg aaaaaggttc
    85921 aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa aacgatcgaa
    85981 acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac ttctcttttc
    86041 tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat aatttcctcc
    86101 aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc aaattcttga
    86161 taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat ttcttttgga
    86221 aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag ctgactaacc
    86281 tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt gcgatgtctg
    86341 ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac agcagtgtaa
    86401 taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac taattttgaa
    86461 tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa acgtttagct
    86521 aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt gcccaagacc
    86581 ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa tggcagaagt
    86641 aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg tgcgcgatga
    86701 agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta cacttgcaca
    86761 tacatttgat atcggctaac gatataatct tccactgcat gcatgccact catggaaaaa
    86821 gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc aaatgttcca
    86881 tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc tgcatcgatc
    86941 tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat cacactagct
    87001 accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc tgtttctgga
    87061 gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatatg ttcaaatgta
    87121 tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt caccagccgt
    87181 tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg agaaaagata
    87241 tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg gctatgttct
    87301 gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag acgttggact
    87361 tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata gttatggact
    87421 ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt tgtcataatg
    87481 tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc attgctccag
    87541 ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag aatacagctt
    87601 ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt ggtcaagcag
    87661 ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg taccaaagtc
    87721 ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccgcgttttt cctgattacc
    87781 gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct gagcgatacc
    87841 ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag aatgggtgat
    87901 ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga gcatttgctc
    87961 gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca aagaaatatt
    88021 caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca ctggagcgta
    88081 acctgcatct ttcagtgttt ggatcccgtc acttagaaaa ggagttcgaa catccttcat
    88141 acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct ggtgttcttt
    88201 agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt tttgataaat
    88261 gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata aattcatatt
    88321 ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt ttctgtaaaa
    88381 atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat gagaagtaaa
    88441 ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt gcgtacaaaa
    88501 gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca aatggtgaca
    88561 attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg cgatgagatt
    88621 cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc aggagaaatg
    88681 cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg aacaccatac
    88741 ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa agaccgtccc
    88801 acaggaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa aatcggcgaa
    88861 tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat tttttgtgtc
    88921 tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga aattagtgta
    88981 tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc gatcttagaa
    89041 caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa ctatcttggg
    89101 aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt gaatattgac
    89161 ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta tgctatcgat
    89221 tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac tccacgtcgc
    89281 cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat cgattacaat
    89341 gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct gtttgatgac
    89401 gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga aatcggagca
    89461 gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga tttaagtatc
    89521 atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga ctctgaaaaa
    89581 gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta tcagaataag
    89641 gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc caagtaaata
    89701 cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt aagtgaggca
    89761 gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc gaaaaatacc
    89821 gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt atcacgatga
    89881 attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt cataaagccc
    89941 tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt tatcggatat
    90001 tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg gacttatttg
    90061 aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag ctccatatga
    90121 ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt tttgaaggat
    90181 ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt aaaacgaaaa
    90241 tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa gagtgccatg
    90301 tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca agcaattccc
    90361 aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc atttataaaa
    90421 gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat cgtaatgatt
    90481 acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga tttacccatc
    90541 ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc tgaaaaaatc
    90601 gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac aattcatgga
    90661 ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga aaaagaatat
    90721 ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat cgtagacgat
    90781 aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag ccatgttgaa
    90841 aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc acttttattc
    90901 aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat ggccgaacaa
    90961 gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga agggacgata
    91021 gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga gcatcaatta
    91081 gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc agaatttgtc
    91141 attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga tcattagacg
    91201 agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat aataagaaga
    91261 gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt attattgctt
    91321 tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc gaagtaaaag
    91381 gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa caatcaacca
    91441 ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa tcagcggaaa
    91501 aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc cgagatccgg
    91561 ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca acaccgcagc
    91621 ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta ggagaaaata
    91681 cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag attggcaatc
    91741 acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg gaggaagtga
    91801 cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt gttcgtccgc
    91861 cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc atcgaatggt
    91921 ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag aagatccaag
    91981 caacagtata cgatggggca attattttat tccatgatat ctatcctgaa acgattcgtg
    92041 ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg acagttgggg
    92101 atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat catcggccag
    92161 ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta gttgaacaca
    92221 taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa aaactgtcca
    92281 ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat tcgtaaatta
    92341 tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca agaaccggaa
    92401 tcttacgtga tatccactac actaaggacg caagcactgg gataattgta actaaacaga
    92461 taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt ttcacaaaaa
    92521 cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat ccaagtttta
    92581 caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga aaatccattg
    92641 gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc atcttcttat
    92701 gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc tttatttgat
    92761 tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa aatcgttctt
    92821 tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga agaagccgga
    92881 aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat tcagcaatta
    92941 gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca aacagaacga
    93001 gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga ggaactggtg
    93061 gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc tgttcagcag
    93121 atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga ccgtagcagc
    93181 gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa tgtacaagtt
    93241 atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt caaacaaatg
    93301 tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca ggaattcgaa
    93361 tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc tcaagtcatt
    93421 gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc tcttacgtta
    93481 aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc agtcaacggg
    93541 aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac tacacgaatt
    93601 cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa gaaaaagctg
    93661 caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa attggttgaa
    93721 ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata tcgtgattta
    93781 ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt caaaagacta
    93841 gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa aactgttaga
    93901 cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata gttaatttct
    93961 ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc caactaagga
    94021 gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata ttatcgaagt
    94081 gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga ttgggcgtcg
    94141 aagtttatcg gaaagtatgg ggatcacaga acgagtctta cgaacagaaa cagacctttt
    94201 gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa ctaagggcga
    94261 agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc atcaaaccga
    94321 acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag gaaacagcga
    94381 agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagcct tagaccgact
    94441 acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg ctgtggtagc
    94501 cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc ctgcaagagg
    94561 cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa tggctgctaa
    94621 agcaaacggt aaccaccgcg cactttatgt tccggaacaa ttaagtctgg caacttacaa
    94681 ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg aagcgaattg
    94741 tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga tgactgaaaa
    94801 agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct actttttcaa
    94861 tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc aagatttgga
    94921 aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagctaaag caattcgagc
    94981 ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg ctgcaaatga
    95041 gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata aggaggaaat
    95101 cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt ctagcattcc
    95161 gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg acagatgcta
    95221 aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac ggaacagttg
    95281 aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta gctaaccgca
    95341 acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa tgtactggtt
    95401 tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa cgcgttgtta
    95461 tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac cacgaaacat
    95521 taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt ttagctccta
    95581 tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca actatccacg
    95641 cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac ttccgccgtg
    95701 cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa gctatcggtt
    95761 tagtaattcc cgaattgaac ggtaaattag acggagctgc tcaacgtgtt cctgtaccaa
    95821 caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt gacgaaatca
    95881 atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac gaaatcgttt
    95941 cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa actaaagtaa
    96001 tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac gaaatgtcat
    96061 acactgcaca attagtacgt actttagaat acttcgctaa cttataagat agacaaacgt
    96121 acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg cttcttccgc
    96181 tttttttgat aataagttta ttcaggagga aataaaatgg ctaagaaaac agtgaaagat
    96241 atcgatttaa aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc tttgaaagac
    96301 ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa ctacgttcta
    96361 gaacaaggcg gaaaagcaat ccttttctct caccttggac gtgtaaaaac agaagaagat
    96421 aaagaaggaa aatctttagc tccagttgca aaacgtctag gcgaattatt aggtaaagaa
    96481 gtaacattcg tacctgaaac tcgcggagaa caattagaag aagcaatccg caacatgaat
    96541 gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag atattgacgg taaaaaagaa
    96601 agcaaaaatg atacagaact aggtaaatac tgggcttcat taggcgatgt atttgttaac
    96661 gatgctttcg gtacagctca ccgtgcacat gcttctaacg taggtatcgc ttcaactggt
    96721 atcccaacag ttgctggatt cttgatggaa aaagaaatca agttcatcgg tgaagcagta
    96781 gaagaaccaa aacgaccaat gattgcaatc cttggtggcg caaaagtttc tgataaaatc
    96841 ggcgtaatcg aaaacttgat tcctaaagca gataaaatct tgatcggtgg cggaatgact
    96901 tacacattct acgaagcaaa aggaatcaag atcggtaact ctctagtaga agctgataaa
    96961 gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt gccaatcgac
    97021 aatgtctgtg caccagaatt ttcaaatgat gttgaaacac aagtaatcga aggagacatt
    97081 cctgatggtt tgatggcttt agacatcggc ccagcatcag taaaattatt tgctgataca
    97141 ttacaaggtg caaaaacagt tgtttggaac ggacctatgg gtgtatttga aatgagtaac
    97201 tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg cgcaacaaca
    97261 atcatcggtg gcggagactc agccgcagca gcagaacaat taggatttgc tgacaaattc
    97321 acacacatct caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa agaattacca
    97381 ggactagcag caatcaacga taaataaacg accaagtttg ctcaattctg acgagtaatc
    97441 gaacaaaagg tgaagggagt tttcatgact ccctagccat ttttcaataa attaaggagt
    97501 gtgttcctca atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca aaactgcatc
    97561 agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg acaaagttga
    97621 ttcagtcatc ggatctcctg cattattctt acaagaatta atgacatcag ctgaaggtac
    97681 agaactaaaa atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt tcactggtga
    97741 aacttcacca gcagctcttg ctgatctagg tgttcattat gtgatcatcg gacattcaga
    97801 acgtcgtgaa tatttccatg aaacagacga agacatcaat aaaaaagcga aagctatctt
    97861 tgcaaatggt atgacaccaa tcctatgctg cggcgaatct ttagaaacct atgaagcagg
    97921 caaaactgct gaatggatcg aaggacaaat tacagctggt ttagctgatc tttcagctga
    97981 acaggtgtca aacatggtta ttgcttatga accaatctgg gcaatcggta ctggtaaatc
    98041 agctgacgca caaatcgctg acgaaatttg tggagttgta cgtcaaacag ttgaaaaact
    98101 ttatggaaaa gaagtatcag aagcagttcg tatccaatac ggcggttctg taaaaccaga
    98161 aaatattgct gaatacatgg caaaagaaaa tgttgacggt gctttagtcg gtggcgcaag
    98221 cttgcaagca gattcattct tgtcattgtt agacgctgta aaataattgt tgaaaatttg
    98281 cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta actaaaatcg tatatgaggg
    98341 aaatccctta ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt attactgatg
    98401 tttacgctcg cgagatctta gactcacgtg gtaacccaac aatcgaagta gaagtataca
    98461 cagaaagcgg agcttttggc cgcggaatgg ttccttcagg tgcttcaact ggtgaatacg
    98521 aagcagttga attacgtgac ggagacaaag ctcgttatgg cggtaaaggt gtaactaaag
    98581 ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat tggctacgat gttcgtgacc
    98641 aaatggctat cgacaaagct atgatcgctt tagacggaac tcctaacaaa ggtaaattag
    98701 gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg tgcagctgct gactacctag
    98761 aagtaccttt ataccactac ctaggcggat tcaacacaaa agtattgcca acaccaatga
    98821 tgaacatcat caacggcgga tcacatgctg ataactcaat cgacttccaa gaattcatga
    98881 tcatgcctgt tggcgctcca acattcaaag aagctctacg tatgggtgct gaagtattcc
    98941 acgcattagc ttcaatcttg aaagctcgcg gattagcaac ttctgttggt gacgaaggtg
    99001 gatttgctcc aaaccttggt tcaaacgaag aaggttttga agtaatcatc gaagcaatcg
    99061 aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc tatggatgct gcttcttcag
    99121 aattctacga caaagaaaaa ggtgtttacg tactagctga ctcaggcgaa ggcgaaaaaa
    99181 caactgacga aatgatcaaa ttctacgaag aattagtttc taaatatcca atcatctcaa
    99241 tcgaagatgg attagacgaa aacgactggg acggattcaa gaaattaact gacgtattag
    99301 gcgacaaagt tcaattagtt ggtgacgact tgttcgtaac aaacactcaa aaattatctg
    99361 aaggtatcga aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa atcggtacat
    99421 taacagaaac tttcgaagct atcgaaatgg ctaaagaagc tggctacaca gcagttgtat
    99481 ctcaccgttc tggtgaaaca gaagattcaa caatctctga tatcgctgtt gcaacaaacg
    99541 ctggccaaat caaaactggt tctctatcac gtactgaccg tattgctaaa tacaaccaat
    99601 tactacgtat cgaagaccaa cttggtgaag ttgcagaata caaaggtttg aaatctttct
    99661 acaacttaaa aaacaaataa ttcttttcta atgaagatac atgatcgact attgaaagat
    99721 agtcgagtat gatcacttag ttaagtaatc gaactctcta cttgctgaaa tatcagcagg
    99781 tagagagttt ttttgcaact agcttctgta tatgatgttt acaaagaatg gtacaagaaa
    99841 agatcatcct tgaaaaagga aagctaacaa taactagcat catataagag cttttaagga
    99901 tagaacagta gaataggaat ctatgggcaa gtcctctcgt ataaa
//
LOCUS       DACDVT010000002        89636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDVT010000002 DACDVT010000000
VERSION     DACDVT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.002"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(45..1085)
                     /locus_tag="IWL52_000100"
     CDS             complement(45..1085)
                     /locus_tag="IWL52_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9776696.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1362..3164)
                     /locus_tag="IWL52_000101"
     CDS             complement(1362..3164)
                     /locus_tag="IWL52_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9776697.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3178..3930)
                     /locus_tag="IWL52_000102"
     CDS             complement(3178..3930)
                     /locus_tag="IWL52_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776698.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3939..4301)
                     /locus_tag="IWL52_000103"
     CDS             complement(3939..4301)
                     /locus_tag="IWL52_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9776699.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4477..5349)
                     /locus_tag="IWL52_000104"
     CDS             complement(4477..5349)
                     /locus_tag="IWL52_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9776700.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5400..5888)
                     /locus_tag="IWL52_000105"
     CDS             complement(5400..5888)
                     /locus_tag="IWL52_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9776701.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(6026..6817)
                     /locus_tag="IWL52_000106"
     CDS             complement(6026..6817)
                     /locus_tag="IWL52_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HAP9776702.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GEFYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6872..7480)
                     /locus_tag="IWL52_000107"
     CDS             complement(6872..7480)
                     /locus_tag="IWL52_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9776703.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7473..7790)
                     /locus_tag="IWL52_000108"
     CDS             complement(7473..7790)
                     /locus_tag="IWL52_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9776704.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7946..8674)
                     /locus_tag="IWL52_000109"
     CDS             complement(7946..8674)
                     /locus_tag="IWL52_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9776705.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8833..9714
                     /locus_tag="IWL52_000110"
     CDS             8833..9714
                     /locus_tag="IWL52_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9776706.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9951..10994)
                     /locus_tag="IWL52_000111"
     CDS             complement(9951..10994)
                     /locus_tag="IWL52_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9776707.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10997..11665)
                     /locus_tag="IWL52_000112"
     CDS             complement(10997..11665)
                     /locus_tag="IWL52_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9776708.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11807..14143
                     /locus_tag="IWL52_000113"
     CDS             11807..14143
                     /locus_tag="IWL52_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9776709.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14258..14569)
                     /locus_tag="IWL52_000114"
     CDS             complement(14258..14569)
                     /locus_tag="IWL52_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776710.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14750..16225
                     /locus_tag="IWL52_000115"
     CDS             14750..16225
                     /locus_tag="IWL52_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9776711.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16272..16856
                     /locus_tag="IWL52_000116"
     CDS             16272..16856
                     /locus_tag="IWL52_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9776712.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16858..17277
                     /locus_tag="IWL52_000117"
     CDS             16858..17277
                     /locus_tag="IWL52_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9776713.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17558..17842)
                     /locus_tag="IWL52_000118"
     CDS             complement(17558..17842)
                     /locus_tag="IWL52_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9776714.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18194..18904)
                     /locus_tag="IWL52_000119"
     CDS             complement(18194..18904)
                     /locus_tag="IWL52_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9776715.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18901..20373)
                     /locus_tag="IWL52_000120"
     CDS             complement(18901..20373)
                     /locus_tag="IWL52_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9776716.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20511..20984
                     /locus_tag="IWL52_000121"
     CDS             20511..20984
                     /locus_tag="IWL52_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9776717.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21137..21688)
                     /locus_tag="IWL52_000122"
     CDS             complement(21137..21688)
                     /locus_tag="IWL52_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9776718.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21793..22323
                     /locus_tag="IWL52_000123"
     CDS             21793..22323
                     /locus_tag="IWL52_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9776719.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22806..23249)
                     /locus_tag="IWL52_000124"
     CDS             complement(22806..23249)
                     /locus_tag="IWL52_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9776720.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23265..24425)
                     /locus_tag="IWL52_000125"
     CDS             complement(23265..24425)
                     /locus_tag="IWL52_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9776721.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24932..26632
                     /locus_tag="IWL52_000126"
     CDS             24932..26632
                     /locus_tag="IWL52_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776722.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26734..27567)
                     /locus_tag="IWL52_000127"
     CDS             complement(26734..27567)
                     /locus_tag="IWL52_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9776723.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27871..30036)
                     /locus_tag="IWL52_000128"
     CDS             complement(27871..30036)
                     /locus_tag="IWL52_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9776724.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30296..32590
                     /locus_tag="IWL52_000129"
     CDS             30296..32590
                     /locus_tag="IWL52_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9776725.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32583..33248
                     /gene="pgmB"
                     /locus_tag="IWL52_000130"
     CDS             32583..33248
                     /gene="pgmB"
                     /locus_tag="IWL52_000130"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9776726.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33241..34248
                     /locus_tag="IWL52_000131"
     CDS             33241..34248
                     /locus_tag="IWL52_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9776727.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34270..35289
                     /locus_tag="IWL52_000132"
     CDS             34270..35289
                     /locus_tag="IWL52_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9776728.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35570..36937)
                     /gene="nhaC"
                     /locus_tag="IWL52_000133"
     CDS             complement(35570..36937)
                     /gene="nhaC"
                     /locus_tag="IWL52_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9776729.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37046..38467)
                     /locus_tag="IWL52_000134"
     CDS             complement(37046..38467)
                     /locus_tag="IWL52_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9776730.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38710..40587)
                     /gene="tdc"
                     /locus_tag="IWL52_000135"
     CDS             complement(38710..40587)
                     /gene="tdc"
                     /locus_tag="IWL52_000135"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9776731.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40878..42134)
                     /locus_tag="IWL52_000136"
     CDS             complement(40878..42134)
                     /locus_tag="IWL52_000136"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9776732.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            42714..44015
                     /locus_tag="IWL52_000137"
     CDS             42714..44015
                     /locus_tag="IWL52_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9776733.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44084..44683)
                     /locus_tag="IWL52_000138"
     CDS             complement(44084..44683)
                     /locus_tag="IWL52_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9776734.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44937..45818
                     /locus_tag="IWL52_000139"
     CDS             44937..45818
                     /locus_tag="IWL52_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776735.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45831..47441
                     /locus_tag="IWL52_000140"
     CDS             45831..47441
                     /locus_tag="IWL52_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9776736.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47518..48177)
                     /locus_tag="IWL52_000141"
     CDS             complement(47518..48177)
                     /locus_tag="IWL52_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9776737.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(48460..49125)
                     /locus_tag="IWL52_000142"
     CDS             complement(48460..49125)
                     /locus_tag="IWL52_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776738.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(49272..49877)
                     /gene="dhaL"
                     /locus_tag="IWL52_000143"
     CDS             complement(49272..49877)
                     /gene="dhaL"
                     /locus_tag="IWL52_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9776739.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(49905..50888)
                     /gene="dhaK"
                     /locus_tag="IWL52_000144"
     CDS             complement(49905..50888)
                     /gene="dhaK"
                     /locus_tag="IWL52_000144"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9776740.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(50890..51285)
                     /gene="dhaM"
                     /locus_tag="IWL52_000145"
     CDS             complement(50890..51285)
                     /gene="dhaM"
                     /locus_tag="IWL52_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9776741.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            51439..51759
                     /locus_tag="IWL52_000146"
     CDS             51439..51759
                     /locus_tag="IWL52_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9776742.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(51871..52740)
                     /locus_tag="IWL52_000147"
     CDS             complement(51871..52740)
                     /locus_tag="IWL52_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9776743.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(52836..54140)
                     /locus_tag="IWL52_000148"
     CDS             complement(52836..54140)
                     /locus_tag="IWL52_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9776744.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(54137..54646)
                     /locus_tag="IWL52_000149"
     CDS             complement(54137..54646)
                     /locus_tag="IWL52_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776745.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(54821..56956)
                     /locus_tag="IWL52_000150"
     CDS             complement(54821..56956)
                     /locus_tag="IWL52_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9776746.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(57039..58694)
                     /locus_tag="IWL52_000151"
     CDS             complement(57039..58694)
                     /locus_tag="IWL52_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9776747.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(58696..59010)
                     /locus_tag="IWL52_000152"
     CDS             complement(58696..59010)
                     /locus_tag="IWL52_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9776748.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            59180..59440
                     /locus_tag="IWL52_000153"
     CDS             59180..59440
                     /locus_tag="IWL52_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9776749.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            59454..59768
                     /locus_tag="IWL52_000154"
     CDS             59454..59768
                     /locus_tag="IWL52_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9776750.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(60498..61052)
                     /locus_tag="IWL52_000155"
     CDS             complement(60498..61052)
                     /locus_tag="IWL52_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9776751.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(61178..63107)
                     /locus_tag="IWL52_000156"
                     /pseudo
     CDS             complement(61178..63107)
                     /locus_tag="IWL52_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(63413..64129)
                     /locus_tag="IWL52_000157"
     CDS             complement(63413..64129)
                     /locus_tag="IWL52_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9776752.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(64130..65953)
                     /gene="glpO"
                     /locus_tag="IWL52_000158"
     CDS             complement(64130..65953)
                     /gene="glpO"
                     /locus_tag="IWL52_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9776753.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(65967..67463)
                     /gene="glpK"
                     /locus_tag="IWL52_000159"
     CDS             complement(65967..67463)
                     /gene="glpK"
                     /locus_tag="IWL52_000159"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9776754.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(67599..69059)
                     /locus_tag="IWL52_000160"
     CDS             complement(67599..69059)
                     /locus_tag="IWL52_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9776755.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(69049..70344)
                     /locus_tag="IWL52_000161"
     CDS             complement(69049..70344)
                     /locus_tag="IWL52_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9776756.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(70676..71353)
                     /locus_tag="IWL52_000162"
     CDS             complement(70676..71353)
                     /locus_tag="IWL52_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9776757.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(71383..72474)
                     /locus_tag="IWL52_000163"
     CDS             complement(71383..72474)
                     /locus_tag="IWL52_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9776758.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(72575..73009)
                     /locus_tag="IWL52_000164"
     CDS             complement(72575..73009)
                     /locus_tag="IWL52_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9776759.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(73593..75050)
                     /gene="ascB"
                     /locus_tag="IWL52_000165"
     CDS             complement(73593..75050)
                     /gene="ascB"
                     /locus_tag="IWL52_000165"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9776760.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(75047..76942)
                     /locus_tag="IWL52_000166"
     CDS             complement(75047..76942)
                     /locus_tag="IWL52_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9776761.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(77000..78775)
                     /locus_tag="IWL52_000167"
     CDS             complement(77000..78775)
                     /locus_tag="IWL52_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9776762.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLDESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(78825..80321)
                     /locus_tag="IWL52_000168"
     CDS             complement(78825..80321)
                     /locus_tag="IWL52_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9776763.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(80515..82137)
                     /locus_tag="IWL52_000169"
     CDS             complement(80515..82137)
                     /locus_tag="IWL52_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9776764.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(82465..83778)
                     /locus_tag="IWL52_000170"
     CDS             complement(82465..83778)
                     /locus_tag="IWL52_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9776765.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(84023..84247)
                     /locus_tag="IWL52_000171"
     CDS             complement(84023..84247)
                     /locus_tag="IWL52_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776766.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(84267..84635)
                     /locus_tag="IWL52_000172"
     CDS             complement(84267..84635)
                     /locus_tag="IWL52_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9776767.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(84832..85542)
                     /locus_tag="IWL52_000173"
     CDS             complement(84832..85542)
                     /locus_tag="IWL52_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776768.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(85560..86057)
                     /locus_tag="IWL52_000174"
     CDS             complement(85560..86057)
                     /locus_tag="IWL52_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9776769.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(86094..86546)
                     /locus_tag="IWL52_000175"
     CDS             complement(86094..86546)
                     /locus_tag="IWL52_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9776770.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(86681..87286)
                     /locus_tag="IWL52_000176"
     CDS             complement(86681..87286)
                     /locus_tag="IWL52_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9776771.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(87736..88356)
                     /locus_tag="IWL52_000177"
     CDS             complement(87736..88356)
                     /locus_tag="IWL52_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9776772.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(88358..89227)
                     /locus_tag="IWL52_000178"
     CDS             complement(88358..89227)
                     /locus_tag="IWL52_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9776773.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(89250..89612)
                     /locus_tag="IWL52_000179"
     CDS             complement(89250..89612)
                     /locus_tag="IWL52_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9776774.1"
                     /translation="MAKEEKALSPEATEKIQSEITQLKQEISTTQDKHKLAKLYEQVG
                     LKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRADAARAGDDQGIDYYMG
                     KMDEMRQIAKQVTIKGNK"
BASE COUNT        26167 a        18785 c        15574 g        29110 t
ORIGIN      
        1 taaaaagggg atgtgacact gtcacactcc ctttttaatt tattctattt ctttatattt
       61 attttgtatt ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac atctccaaaa
      121 ctttcaagtc gctccgctcc tggcagttct tgagtctcaa atgtgatacc gccatgattt
      181 actaatttat tcccgcgcat ttccggtcca tcttctccaa aatttgctgt aaaaatcacg
      241 attcctgaag catctgtttt tacttggatc gatacttttt catctgggct tgtcaattcc
      301 gcagcattta gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat gccgcccact
      361 atcattgatt ggcgaaaatc actggcaaag acctcaccta attttttagg acgttggaag
      421 tcaaatgctg tgccacttac tgaaactact tctcctgtag gcgttgtatc tttattgagc
      481 gtagcaaata atcggctatt caaccaaagt atatgttcgt cgattgactg tgctggatca
      541 ccggttaaat tgaaatagac atgatttgta ggattgaata ctgttttgcg gtcactgatc
      601 ccttggaccg tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac ttctacatct
      661 aaagttccag gaaatccgtt ttccatatct gggctagtct gatagaaaat gaccgatgct
      721 tcgttttcac cattgatgac tgtataatgc cactgcttgg cttcaaagct aggtgcacca
      781 ccatgtaaac aatgaccagt gtccgggtct gtttttactt cgaacgtttc atcatctaat
      841 ttgaatgtgc cattcgtaat ccgtccagcc atacgtccga ttgaagcacc gatataaggg
      901 tctttttcta gatattcttt tgcttcatca aaacctaaga tcaactctcg tttttcatta
      961 tcgattattg tttgaaaatg aacaacccgt gccccaagat cactgacaga caaagatgtc
     1021 ccattttgat tcgtgatcgt aatcaagtga tagttttcgc caaaattttg ttcattgatt
     1081 tccatgttcc ttgcaccctc ttttctttca tttgcactga ttccagcata catggaaacc
     1141 gttttcttaa cagcttattc cgtgagtaac gagaaaaaat ttactatatt ctaataaaag
     1201 accgtgaaaa aaaggttgtg acaaaaatat tgtcacaacc tctttttccg aacaaacggt
     1261 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg aggagctatt
     1321 tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaatct ctttttcaat
     1381 acgattccag tatataacca tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat
     1441 atcgtaaacg gaacccaaat atctttgtat ggattttgca tgaatagacc aaacatttgt
     1501 aatccgaata agacatggac tacacctaag attcctggtg ctaagaataa cacgcctact
     1561 tctctacgga tcgattgttt aagcaacccg cgtcgtgttc ctatcttttc aagcatttca
     1621 taacgagcaa tgtcactctt gcttcctgac aagatcttga acatcaaaca gctagcaagc
     1681 atggtcaaga aagctaaacc taggaagaat cccataaatt caaaaccaga gaatagacca
     1741 ttatacactt catacatttc atatttctgg ttgctgtact ctactttttg caaagatgga
     1801 ttgttcattt tattttgagc aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct
     1861 tttacctgaa ctagctgtaa agtggattgt ggcaagttca gttgatcaaa ttctgattga
     1921 ctgactacct tgatttcttt ttcttgctgt tccggcaatt catagcttcg aagttcctct
     1981 tgtacagtca gttcattcat ttttttagca ggaacttttt ctttcgtgat cttcggcggt
     2041 gttccatctt catgttttat catgatcaat ggttgagccg caaattcact tgcattatag
     2101 taaatcgttc ccgcatcttc tttttgagta taagaagcag aaagtgttgg tgaaagttct
     2161 ttgatttttt gctgatcttc tgcccgagga ttattcatca ctagatcata tccagaattg
     2221 ttttctgtca agtctgcaat ttcattttta aaacctaatc ctaccgtcaa agcgcccagt
     2281 gccaaggcaa acagcatagc gaccattgag agcatttgtg tatattcacg aatacgaaag
     2341 tttaactgtg acaatgtgaa attgttcaat tttttcaatg aaatctgttc tgatttcttt
     2401 agtaatgata aaaagaaaat gatcacagaa tggaaaatga aataagttcc taatacgatc
     2461 gtaactaagg ctacccctat accaaagact tgataggtcc ccacgtgttt catcatatag
     2521 taaccgcagc ctagaaaagc aattcccagt actacttcaa ggaaaaacaa gaatttatgc
     2581 tgcttgattc ttgttggtgt ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca
     2641 atcgaaaaag cattgacaac agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg
     2701 atcaacaacc cgtgaatact aaaaggtgta aaatgcgaaa tattgagatc taactgatcc
     2761 actaataatc ggtggacgat acttgtcaat cctacaccga cagctgaacc cactaaagta
     2821 gctgcgatcc ctactaagaa tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt
     2881 gctcccagca tcataaacat tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt
     2941 gcatataaaa tataaacgaa tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg
     3001 ataactgtag ccgcaacgat cgtattactt ttcaaaaaag cttcgttgct tgccattgat
     3061 tcaaacatgt aaaaaatagc agatgccata actaatcctg agaataacac caagtagtct
     3121 cttaaacggc tcttgatccc agtaagtgat aatttccaaa gcatagtcaa tctcccccta
     3181 ctgttccaag tttccaagaa tagatagtat ttctcgatag aaggaatcac ggctttgatc
     3241 tccacgtttc acttcttgat ggataccgcc atccttgatg aataaaatac gttggcaata
     3301 acttgcagaa aacggatcat gcgtcacaag aagaatcgat acatgatctt ttgtattcaa
     3361 ttcgtccatc gtatctaaaa gatcccgggc acttttggaa tccagcgcac ctgtaggctc
     3421 atcccctaat agaatcgttg gttgtgtgat caatgcacgt gcggcggcga cccgttgttt
     3481 ttgtccaccg gaaatctcag aaggatagct ttccaaaata tgagagatgg ataaacgttc
     3541 tgcaattttt tggacacgtt gcttgatttc ttttggtctg acgccttgta aagaaagagg
     3601 aacagcgata ttttcttgtg ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa
     3661 gccaatctca ttcgcacgaa aatctgctag ttgatttcct tttaatgtgg tcacatcttt
     3721 ttgattgatt cggatatgcc catctgttgg tttatctaat gtggataaaa tattcaaaag
     3781 tgtcgactta ccagaaccag aagcgcccat gatcccaata aattcgcctt tttctacatc
     3841 gaagctgatg ccgcttaatg cttgcgtctt tttctcattg ttttttccgt atacttttgt
     3901 tacattctgt acttcgacta ttttgttcat ttctttccct accctttaat tagtttttcc
     3961 agtgctgttt tcggcacgtc ttttttttcg acttcttcat acgaagtgac acctttattt
     4021 ttgttccagg tgatttttaa atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg
     4081 aagtccagtt ctttttcctt accctctttc gtatagccag gcaggatata tctatagttg
     4141 actgtttctt gtccagagtc tgattttgaa actgtttttg taccatctgt cgtaatttgt
     4201 acatagtatt cttctccacc cataactacg taatctgcga ttttcacccc tgcaagtacc
     4261 acaacgatga aaacaagtag accgatcaga agttttttca ctttttcccc ttctctctta
     4321 ctttatatac tgattataga aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct
     4381 tctttcaatt ttgtcatttt ctttaaaaca actaaaaaaa gagttcaaga caataatccg
     4441 tcctaaactc cttcttccta tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc
     4501 tgtattgata acaggttgcg ttcctttgtc tgtaatgatt gagacaagta aattattgat
     4561 caattggact ttccgatcat cggtgaaatt gatttcttgt ccatcttcga tttgctctaa
     4621 ggccatttga gtgattgtga cagctccttc aacaatggtt tgtcgagcag acaaaatggc
     4681 ttttgcttgt tgacgttgga gcatggcact agcgatttct gttgcatagg ctaaatggtt
     4741 caagcgtgtt tcgattactt cgacacctgc tactgctaaa cgctcttgca gctctttttg
     4801 caactcgtca gacactgccg tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt
     4861 atcatacggg tattggctag cgatatgccg aatggctgtc tcactttgga tttctacaaa
     4921 atcttgataa tacgctacat cgaataatgc ctttgctgta tcaacgactt taaagactac
     4981 tactgctgag atttcgatgg ggtttccatc caaatcattg acttttaaaa cagaactatt
     5041 gaaattccga actttcaacg aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa
     5101 gccattttct cgaatcgtac ctaaatattg tccaaaaaac aaaattactt ttgcttgatt
     5161 aggactgact acagttgccg aacttaacaa caataacgtg atcaaccaaa gaaaaatact
     5221 taaaaaaagt gcccaaactt tcgcttgcca catacctaaa tagaagaaaa acaatccaac
     5281 taaagctaat acgaccaatc cgacaatccc tgcataacca ttgatataaa atgctctttt
     5341 ttctctcatg ctacttcctg cgctccttta tccttcaata aatatctaat gttacttttt
     5401 taattttttt ttcgaatacg cgccattaca ccttctagtt ctaactcttt gtcttcagtg
     5461 atgtaccaac cgcttgcctg tgtccctccg gctgcctgcg gtaaataatc caattttgtt
     5521 cgaacccagt gcttttcttc tgtaaaatag tcttctacca agacctctag atccatccct
     5581 gctgaaagag gcaaatcttc ccgaacagat ttaaagataa atcgttcctt cttcccatca
     5641 tacttcaata ttcctgtatc gataatcggt aaagaataat aatccagctc tttcttcacc
     5701 gtcaataaat gtttgaaaac ttcatggtac acttgctcta attggcgacc ttctgaattg
     5761 ttcgggtaat taaccatcac tttttctttg tctggatcat agaaggtttg gcttagttga
     5821 gtgatataag tatgtaatct ttccatttct gtttttaact tttctctttg accttcttct
     5881 accttcatat actctttcac cctgttttct ttttagatat tttctttctt attttactat
     5941 agttatgtgc gttaagcgat gaaaaacagc aaagattgga aggatctttg ctgttttttc
     6001 ttaaaatatc cagttgagta caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa
     6061 tattgatcga tgtgtcttta gcagtgatct ttagcttttt ctgtccttgt tctaacaaag
     6121 tatttgttat tggctggtag tctttttcaa tataaaattc accagacgtt tggatagtat
     6181 agccactgtt ttccatctca tgcaaagata aattgccacc gtttaggatg acttttcctt
     6241 ctttgctgat caaactgtct tctagatgga agttgctgtt ttcacctgtg acgatcaagt
     6301 tatccacatt ggcctcatct aatcgaagtg agcctaattc atctttcgca gttattgttc
     6361 ctcctgtaaa ttcattgaac gatacccagc catcatgtgt cttagtattc acttttttga
     6421 ttgatacttc gttgatatca attgcactat tattcccttc gattgtcaat tcagataatt
     6481 catgatctgc agggacaaca atagtgattt gtgcacttgt gtcttctaaa ctaatgattg
     6541 cacctgttga ttttttgact gcattcacag tcaacacgcc atttttctcc tttatttctg
     6601 gcttttctgt cttatcataa gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa
     6661 ctgtttgatt ttctgcatgg atgacgattt tgtttgcatc cttgattttt tttgtctcag
     6721 taaccatatc gatcaaccga ggaccattat cccaagtcag actcttttga gcgcctaatg
     6781 tataagttgc acctgctagg aggccaccag taagcatcag accaataccg gtaatcaaaa
     6841 aatgtttcaa tctcaattta tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt
     6901 ttgtaataaa acgtcgtccc atccatttaa caaagttcat catgacataa aaaagccctt
     6961 ttgttccttt cagcaacagt ggcgaaataa atactgcgct tcctgtggcg aacagaccga
     7021 caccgataaa gaataatcca ctcagaatcg attgtccaaa cacactgatt cctgagatga
     7081 tcatgaatag gccaacacct aagatactga tacataaaac atagatacta aaaatgagcg
     7141 ctagtaaagc aaccaatagt cccccaataa ccaatataaa ccccagtgct agtggaatga
     7201 ggacaggaga agcaaataaa ccgataatga ttaaccaaat caggcgaatt tgtctttttg
     7261 gatttttcac taactcgttt tctgcttgtt cttcttctga aaagtaactg atttttaatt
     7321 ttaaggcaaa ctgctttggt gtaccatatt catcaatgat ctgatctaat gtttttccag
     7381 aatctaaaaa atattcctca taatactgga tcaaatcaaa acgatcgtct tcaggaattt
     7441 cttgaagatg atccacaact tcattcaaat attcattcat ttgtgctctc ctccaataac
     7501 cggtctaaag aatctcgaaa aacaatccat tcatcaataa tttcttctaa atgacgactt
     7561 cccattggtg tcaacgagta atatcgacgg tttctgcctt caaaaggttc atcataggtc
     7621 tcaagatatc cttctttttt caatcttctc aaaaccggat atagtgtaga ttcactgaca
     7681 gagagacttt cttgtacgat ttttgttaag gcatacccat acatatcttc cttgcttaat
     7741 gctgctagca cagtaccatc aagaagaaca gttggaatct ggatgtgcat tacgtcacct
     7801 tctattctca agtgtaatat tttatttcaa tctatattat acaacatata atatagtttc
     7861 aaaatcagtt tgtcaaatac aaaaaaactg actaaaagat ttgatccttt ttagtcagca
     7921 aaaaatggct tctctttgat ttttattata acagtggcat aatccaattt gtccacatcc
     7981 agctcagtgg catgataacg atcattgcag gtatcatgtc agctactggg aacattttca
     8041 cgttgatcat tctaaaaccg gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta
     8101 agatcatgtc tggtgtagtc aatggataaa taaattttgc caataagaac aaaatataaa
     8161 aaataatgaa ttggggaatt gctatcacag aaacgacata ccctaaatta cacgcaaaaa
     8221 tagctgctgt gaaaaaatca aggacagatt tagaaatcaa aatcgtactg tctcctgtca
     8281 tcccagaatc taaacttcca tagatccccg taccacttgc acaaaataga acaatgaccg
     8341 tcactaatgt gctgataaat tcatcgtggg tcataccaag cttttcacta gggaagattt
     8401 tagaaatagg tacttgcatc agcgcagccc ccttatttat cccattacct aaatgaacaa
     8461 tcaaaccaat cgctgttcct atcactaaag caaaaataac agccggcata tttttcatcg
     8521 gcgcaatgga atagattccc atccccatcg aacacacacc aaaaaccatc gtcagctgtg
     8581 ctttaaaatg ttcacttaat ttatttccta ctaatccgcc aattagtccg ccaaacaaaa
     8641 cggaacctac attgatcaaa actccagttg gcatcaaaaa actcctctct ttttctacga
     8701 agcgaaaaaa atcagaaaaa tttctgtaat tattgctttc gctgcaagaa agattgtaac
     8761 agacagagaa gattacacca attcattttt tgtgttaaag tattctttat tggaataagt
     8821 aggaggaata tgatggatat ccataaattc gaagtttttc ttgatttagc cgaaacaatg
     8881 aatttcacca aaactgctga tcgacaattc accacccaag gaaatatttc taaacagatt
     8941 ctttcgttag aaacagaatt agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa
     9001 ttgaccgaag ctggaagctt attgctccca tatgtaaaaa atgtcgtaga acaataccat
     9061 gctatgcaag aagtgcttga aacctatacg aaagacaaca atctttcatt aaatatctta
     9121 acgataccga caatgatcaa ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc
     9181 catcctgaat ttacagtcca gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac
     9241 gccaatgaac atagtgacac gatccacttt gctagaagtt tccaggcccc accaagttcg
     9301 attgagtggt tgcctactga aacagacgat tttgtagcag ttctacctaa aaatcatcct
     9361 ttagctagta aaaagaaatt ggatctaagc gaactgaaaa aggagaactt tttgctcctt
     9421 ggccccacta cgaacttgta tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa
     9481 ccaaaaatct cttataaagg agcaagaatc gacttgatca tcaatatgat tgcaaatgac
     9541 atggggattg ctattgttat ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg
     9601 attctcccta tttctccaac aacagagagt tatcttgcat ttactcgtaa agtcggtgag
     9661 cattctttgg cttcagatac attttgggag tatttaaatc aagaagaaaa ctaagcccta
     9721 cattttctgt ccatctatcc acccaaaaaa agagagcgac gcatgacttt cgctctcttt
     9781 cttgcatcat gtccttgttt caatgacacc tctaacattt ctcgtagttc aataagaaag
     9841 cagcgatttt tatgccaaaa gaaccgtaag aacatttccc taatcacaaa aaaacgacta
     9901 aaaaccccta aaaaccttca atagaatttt taggggtttt attctttttc ctattgttta
     9961 cttaaccgta tcattgctgt cgttcctact ccttcgatac tgctataatc aatacgtcca
    10021 tcatgcagtt ctagaatttt tttaaccagt gaaagtccta gcccattccc agaaatttga
    10081 cgacttgtat ctccttgata aaatttttca aacattcgat ctctggtctc ttcattcatt
    10141 cctacacctg aatcggtcac ttccagtaca atctctgtgg ctgtctcaaa tagtttgata
    10201 tgaatctgtc cattgatttg attgtacttt atggcgttat ccatgatatt cagccacact
    10261 tggtacaaaa actcttcatt gcctgtgtaa tttactgcag caagttcgat atccaattct
    10321 agctgttctt tctcccattt aggctgaaaa aaaacgatta cttctctgat ttgttcatct
    10381 aatcgatact ctttcttttc cagttggata cgctggtttt ccagtttgtt caattttaag
    10441 atgttctctg ttaattgtga aagctgcgtg atactttcga taatccgata taaaaatatt
    10501 tggcgttctt cgtcagagag atttggggct tgtaacaatt gaacatatcc ttggattgtt
    10561 gctagtggtg ttttgaattc atgggataca ctggacacaa agtcattcct cagtgtctcg
    10621 atactattga gttcttggac cataacgttg aaatctttat acagttgctg tacttctgct
    10681 actttctgct gttcatccat tcgaatcgaa aaatctcccg ttgctaccag actcatattt
    10741 gtccctagcg cactgattgg atgcaggata cgccgcccaa caaaaatcga tattcctgta
    10801 cccacgacca tactgaaccc cgcaaagacg aataacgggt ataacgttgg ttttccagcc
    10861 gattcagcag gcgagataga atgtcgagac aataagaaaa taaaaccgag aaaacacagc
    10921 agtgtcacta agatcgaaat gaagacaata ctagcaaaat agatccataa ttgtgaaaat
    10981 agcttttttt tcatctttaa ataaccgcct tatagcctag tccccgtact gtgatgattt
    11041 gaaaatcttc cgtcttttca aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc
    11101 gttcttctgt attcgtatct aatccccaaa tatcatccat caactgttgg cgagtgaaga
    11161 tcttgttggg ataagacagt aatttataca gaagcagaaa ttctttttga ggcagaatca
    11221 acgaatgctg atcttgtaac actgtataag catctagctc taattttgtt tgtccgatta
    11281 tcaagctact gctgtaattg atctttgaac gtcgaagcaa tgcttccaca cgtaggatca
    11341 tctcattgac atcgatgggt tttgtcatat aatcatctgc tcctaatttg aacccgcgtt
    11401 tcttatcctc aaaatcggcc tttgccgtaa tcattagaac aggtgtgtca aatctggtgc
    11461 tacgtaatga gtctagcaag tcatagccat ccattttcgg catcatgata tctgtaatga
    11521 ttaattctat acgtgtcgct tctagttttt tcagcgcatc ctctccattc aatgcggtga
    11581 tgactgaaaa gcccgctcgt tccaacacag aacggtaaag caactgtaat tgcggatcat
    11641 cttctactac taaaatacga ttcattgatc tccctctgtt ttttgaaaaa tttctagatt
    11701 ttttcttgta gttcatcttc agttaacatt tatttcgtat actacgatca ttgttctgat
    11761 atacttatta gtataccgaa tatttcaata cgggagaagg gtgattatgc ttcaattaaa
    11821 agatataaaa aaatattata aagtcggcga aaccaccacc aaagcattgg atggtgtgtc
    11881 tgtcgctttc agacaaaaag aattcgtagc gattttaggt cctagtggtt ctggtaaaac
    11941 gactatgctg aatgtgattg gtggactaga taattatgat tccggtgata tggtaatcaa
    12001 cggaaagtct actaaagact ttaaggacag tgactgggat gcctaccgaa acaactctat
    12061 cggtttcatt tttcaaagtt acaacttgat tggtcatcta ggcattattg aaaacgttga
    12121 acttgggatg acattaagtg gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc
    12181 attgcaccga gtcggcttga caaatcatat gcataaaaag ccaaatcagt tatctggtgg
    12241 acaaatgcag cgagtggcca tcgctcgagc actagccaat gacccagata tccttttgtg
    12301 cgatgaacca accggagcgt tagatacaga aacaagtatc cagatcatgg aattgatcca
    12361 agagttatcg aaagaaaaat tagtgatcat ggtcacacat aatcctgaac tggcccatca
    12421 atacgcagat cgaatcattg aattttctga tggtaagatc ttaacagatt cccatcctca
    12481 catcgaacga ccaaaagatg atcaatttaa cttacgcaga acaaagatga gtttttggac
    12541 agcgttaaag ctttccttca acaatatccg aacaaaaaaa ggacgtactt tcttgacctc
    12601 ctttgcttcc agtattggga tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt
    12661 ccaaaaacaa atcgataaca cgcaagctga aacgatggcg aaatttccga ttactatttc
    12721 taaggtaacg actaaccaga ctcgtgacga tgccggattg ggtgcaagca aagcggatta
    12781 tccagacagc aaaacgatta ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa
    12841 taagattgat caaacatacg tcgattacgt aacagacatc gatcctaact taagtaataa
    12901 tatcggattt acccgtacta caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca
    12961 gcctgtcagc ttctcgaatc aaaatcctga tacggaatct ctttctttat ctagtacgat
    13021 gtcagcaatg acaggtgtgg gagtgtcaag tttccctaca cagcttgaca ctagcaaaga
    13081 aaacttttta aaagacaatt attctttact tgccggaagt tatccagcct cagcaacaga
    13141 tgtcgtgctg atcgtagacg gcaataataa tactaatatc aacgcattga agaatcttgg
    13201 atttgatgtg aaagaagacg aaaaacttga ttttgatgat atcgttggta caacattcaa
    13261 attagtaaac aacaatactt actatacaaa acttccaact gggaatttta tccctaatac
    13321 agattatgat gcgatgtatc agaatgcttc tgatgaattg aagatctcgg ggatcctacg
    13381 cgtgaaatct tcttctacaa tgaatctctt atctccagga atcgcttata gcgatcaatt
    13441 aacgactcaa atcgtcaacg aaaacaaaga atcagaaatc gtcaaagctc aaagagacag
    13501 tggtgtaaat gtcttaacta ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag
    13561 ttatttaggt ggagacagct tgccttcaag tatcatgatt tatccgaaca attttgaaga
    13621 caaagaaaag atcttggatt atctagatga ttacaataaa ggcaaatcag atgaagataa
    13681 aatcatttat actgatttag ctggaacgat gactgaactg actggcggat tgatggatgc
    13741 tatcacttat gtccttattg cttttgcagg gatctcttta gtaacaagta tgatcatgat
    13801 cagtatcatt acttatacat ctgtgattga acggacaaaa gaaatcggtg tactaaaagc
    13861 tttaggtgca cgtaaaaaag acatcactcg cgtatttgac gcagaaacgt gtattttagg
    13921 tatttcttct gggatattag gtgtgtttat tgcatggcta gctacgttcc ctatcaatag
    13981 catcttatac aacatgacgg atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat
    14041 tattctagtg atcgtctcta ctatattgac gatgcttggc ggtcatcttc ctgcacgaat
    14101 ggcagcgaaa aaagacgccg ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat
    14161 ttagatttga gtcataagag cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat
    14221 atgttccttg tttcgcgctg ttttttttaa ttatttttta taattctggt atacttttct
    14281 ttcctttgta ccacaactct tctggcgtat acctgtcttt tccatttatg aatactgttc
    14341 catctgcttc aatcgttact tcagtcccaa tccacatatt acgagaaagt gcgagaagat
    14401 tttttctttt tcctcttcct ttttttccaa attgcaaggc atcggttatc tcttctgtcc
    14461 aatgagattt cacgtatttt acttctccgt tttcaaatgt aactttcaaa atattcgcta
    14521 tattttccac ttttactgct ttaggaaatg actcctccat ttttttcact ttccttcctc
    14581 caacactaag tatgcgcttt cttttagtga taagtatatc atagtttatt ctattatcaa
    14641 aaaaacgcat ccattccatt gtagaatact ttaccgaaca agttgaattg ttttcctctt
    14701 tttcaccttt tactatagat aggtaaagac aactaaggag gaatctctca tgacagatca
    14761 accgtatgat ttaagaaaag tcatcgaaga actaaaaaca cttccaggtc aatatcacga
    14821 aacagatatt gaaatcgatc ctgaagcaga attatctggt gtttaccgtt atatcggtgc
    14881 tggcggtaca gtaaaacgtc cgactcaaga aggaccaact atgactttca acaatatcaa
    14941 aggtttcccc ggcacacgtg tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga
    15001 aattttgcac catgattatc gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc
    15061 aatcaaacct gtgaaagtag acaaagcaga cgctccagca caagaagtcg ttcatctagc
    15121 taccgatgca gacttcgata ttcgcaacat tttgcctgca cctacaaata ctcctgaaga
    15181 tgctggtcca tacatcacaa tgggtgtagt cttaggatca gatccagaaa aaacaatgag
    15241 cgatgtaacg atccatcgta tggttctaga agataaagat acgatcggta tgtacatcat
    15301 gcctggcgga cgccacattg gtcacttcca aaaacaattc gaagccttga ataaaccaat
    15361 gcctatcact atcaatattg gtttagatcc agcaattgcc attggcacga ctttcgaacc
    15421 acctaccact ccacttggct acaatgaact atggtgtgca ggagctttgc gtaacgaacc
    15481 tattcaaatc gttgatggta tagcagtcga tgaagtgggt attgctcgtg ctgaattcat
    15541 cattgaagca gaaatcatgc caaatgaaac aatgcaagaa gatatcaaca cgaactcagg
    15601 aaaagcaatg ccagaatttc ctggctatac tggggatgct aatccagctg tacaagtagt
    15661 gaaagtcaaa gcagttactc accgaaaaga caatccaatc atgcaaacaa cgattggtcc
    15721 cagtgaagaa cacgtatcta tggccggtat tcctacagaa gcaagtattc ttgccttggt
    15781 cgataaagct attccaggaa aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa
    15841 attgatgacc atcatgcaaa tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca
    15901 agcagctatt cttgctttat cagccttcaa agaattgaag acagtcattt tagtcgatga
    15961 agatgttgat attttcgaca tgaacgatgt agtttggact ttcaacactc gtttccaagg
    16021 agataaagat atcatcgtga tcccaggaat gaggaaccat ccattggacc catcagaatt
    16081 accagaatat aatcctggct ctattcgttt ccgtgggata agtgcaaaaa ctatctttga
    16141 cggtacagtt cctttcgata tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga
    16201 agattgggaa aaatatttga aataatgaat gatctgcaac tgttttgaca tcataaaaag
    16261 gagtgcggaa aatggaacaa ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg
    16321 gcgcttcagg caccatttat gccatcaacc tagtgaagaa actaaaagaa tatccgatga
    16381 tagaaacaca cggaatcatc agtccatggg caaaacagaa cttaaagctg gaatccgact
    16441 tatcactaac tgaattcgaa agcctgtttg attaccatta ttcaaacaaa gatttaggtg
    16501 ctgctgtcgc cagcggatca tttttgaccg atggaatgat catcgtaccg gcaagtatga
    16561 aaacagtcgc aggtatctct gttggtttag gcgacaacct gattgcacgt gctgctgatg
    16621 taacaatgaa ggagcaacga aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca
    16681 ttcacttgga aaacttgact aaattagcac gactaggtgt gcaaatcata ccaccaattc
    16741 ctgcttttta taatcagcct caaacgatcc aagatcttgt taatcaccat acaatgaaac
    16801 ttttggatca atttgaaatt gtaaatgagc attcaaaacg ttggcagggt gattaatatg
    16861 gaaaaagaaa cgatagttga agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa
    16921 gtaatcggta aagacttatt gatcacttta acaggcggag atacaccaca tatcggaaca
    16981 gtcactacat tttctcgtga tacagagatg caaactgtac gtttccctag ccacgatggt
    17041 cggttccata aagacgatgt cttagccgaa aaaatagctg tgatcatcaa aactgccgtt
    17101 ccgggaagtt gtacgatcac ttccggtatc catgttgacc atatctccaa tgcacaaatc
    17161 aacgcctctt ttccgatgtc agaagagtta ggttacaaga ttttagattg gttggaaaaa
    17221 atagatttcc atatcaaaga accagtttat tataaagatg gtgaaaaacc attatgaaag
    17281 aagaggttgt gaaatcagcg ttatgacctg agtatgagag aaaaaaatgg aaaaatagct
    17341 tctcatattt tgccgttttt gagctaatac cgagggacag ctgattgaac gccgtttatc
    17401 aagaggttgt gataaagccg cttcgtttcg agtagtaaga aaaattttgt aaacatgggt
    17461 ttccacattt ttgcataaat ttgacttact accgagaaac tggcttttga acaccgttta
    17521 tcaagaggtt gtgacacgtc ttttgtcaca acctctttta tttttgcttc actgtaactg
    17581 ctacaatatc accgatttct tttccgatgt ttttacgaat atcttttcgt agtccaagga
    17641 tgtagcagac tgaaccatct ggatttttca cgcccatatt cacgatactg ccttcatatg
    17701 gcacattgtc aaaagttgct tggactttaa ctcttccagc accgaattct tttcttatat
    17761 catacggaaa aataatatat gccccgccct tgccgacttc ggaagcatag atttttgatt
    17821 caaaagaata aatttccctc attttatttc tcctattttt tacatctagt atagcatagc
    17881 aaaaaaaaga aggaactgtg acaaaagtct tgtcgcagtt ccttttttcg aataaacggt
    17941 gttctcctgc caactcctcg gtcacaagtc acaatagtca acaaacatga gaagctgttt
    18001 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc ttccttaaac
    18061 ggtgttcaaa agcttgcatc tacggcacta agcccaatca gatgaaaaat atggagtgct
    18121 atttttatct gattgttctt actgcttgat gcagagcagc tttttcacaa cctcctacat
    18181 ggtgttcaaa atattattcg ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt
    18241 ggaattcagc agctaaggct ttcgtacgtt gttcttgctc tgctttgctc atttttggat
    18301 tttcagtcat tgcttttacc ataccgtctt gaacataagc ttttgcttca ctttccatcg
    18361 tactttgctg tttcttcact aattcagcta acttggctgc ttggtccgta cttaattcta
    18421 cccatgtctc tgggattttg aatgtattca ctcggctgtc atattcgtga ttgttatcag
    18481 caatattgaa ccagaattta tacatgtcgt ttttgtattc ccaataagta gttttcgtca
    18541 ctttttcagc atcgccgtct gtcttttcta ctttatttgt ttcgttatcc gtaccagttg
    18601 cttttggttt ttcttgtttt tcatttgttc ggtaaagata cactttttct tttccattcc
    18661 ctaaagcttt atacaaaagc atatctgctc cttgggaatc agcactggaa acaatttttt
    18721 ctgtttttgt ttccgttact ttttccattc caaagtgatt ggacaaatta gcgacgatca
    18781 aacttagtga agcgacgaag attagtccga aaatcaaaga taaaaaggtt tgccatgttt
    18841 ttttagcgaa cacgttcgtt aaagcaaaag caagcaagct gacaatcaaa actccaataa
    18901 tcatgcatta tcccccgctt tcattagaac aggttctttt ttcttcaata aaaaggcaat
    18961 aataaagcca agaatcccaa agatgatcgc tacgataaac gcggcatggt aacctgacag
    19021 tgtggcattg attgcctgat ctttataagc taatggcgta ttatccaata cagatttgct
    19081 tgggatattt gcttttgtca cattcgtcaa cacactgatc aaaatagctg taccaatcga
    19141 gcttgcgatt tgacggaatg tattatttac cgctgtccca tgactgatca agttatttgg
    19201 caatgcattc ataccagaag tcgttaccgg catcatgacc atggagatcc caaacatacg
    19261 gatcgcataa aggatcacga tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg
    19321 tgcagttgcc gctgtcaaaa tgaacatccc tgtgatggct aaacgtttcg cgccgtattt
    19381 atcgaaaatc gcacccgtca caggcatcat agcccccatg atcaatgcac ccggcaacaa
    19441 catcaagcca gaatggaatg ctgattctcc acgaatattt tgaatataca atggtacgac
    19501 catttctgcc cctaccatcg ccatatttgt tacaccagct aaaacagctg caatcgtgaa
    19561 tgtagatgat ttgaatacac gaagttccaa gaatggatgt tccaaatgta attgacgcca
    19621 tgagaacaag gcgatgaaaa tcacaccaac aactaagaaa ccaattactt tcaagcttcc
    19681 ccagccgtca tctcctacac tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga
    19741 taaaatagcg gataaaatat caatgctagg atttgacaat ggaataacac ttttcattag
    19801 gaagaatgct aaaaccaaga caagaacaac gattggcagt accataccaa acaagtcacg
    19861 ccaatggtag ttatcaatga tataaccaga taatgtaggg cctaaagcag gagcaagtcc
    19921 aataacgatc cctgacatcc ccattgctga tcctcttttt tctggcggga aaatagatag
    19981 catgatattt tgcaataatg gcatcgatac tcccacaccg caagcttgga tcaaacgacc
    20041 gattagtaat gtactaaaat taggtgctgt aaagcaagta atcgttccga tcaaaaagat
    20101 cgtcatggca gaaagatata aatttcttga actgaattta ttgatcagcc atgctgttat
    20161 tgggatcatg atcccgttga ctagcaagaa accagtcgtt aaccattgca catcagacgc
    20221 tgaaatatcg aaagctttca tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa
    20281 aacggtacaa aatgtaccaa ttaataagac aacaactaaa agtgtccgat tatatggttt
    20341 tccgtaaata tctacgggtt ggttgttact catatttctc cttctttctg aattttttct
    20401 gaaactacta gaatacttta gtcctaaatt cgctatttgt caacctactt gacacataag
    20461 ttaatacgga tataattaag ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg
    20521 cttcccttaa acgtttgttg gaaaatgatc aattggttgt tggcatcagt gaattaagtg
    20581 atatcgtagg tgtgtctcca cgtcaattaa ggtattggga acaaaaggga ttcattcgtt
    20641 cgatcgaagc tgatgcgaat tgtcctagaa aatatcgttt acctacagtc ataaaagtag
    20701 aactgatcaa aaaatattta gatgacggat ttactttgtc aaaagctgca gaaaaggcag
    20761 aaatccgcca aaagaaaatg caccatgtcc gtaaagtttt ttcaaaagca atcaaagata
    20821 tcgaattgat cgacgaacgc tatacgattt tacaaatcgg taacttcaaa gaagagaatg
    20881 ctaagatgta catcattcat gacaacgaga cagacgaatt aacttacaag ctccttcctg
    20941 tcgatgaaaa agctgactac gaacagttgt taaaagaact ctaaacgcca ctaatctcct
    21001 aagtaccaag ctttttaata agtagatgct acataaatac ttgaaaaaag gcctgtttga
    21061 acataagaat atccgaacgg tgatctgcgt ttctttcata tagaaaggaa ttctccatca
    21121 ctgttcggat atttttttat ttttttcggt tactttcttc taatacatct gttagccaat
    21181 ttgcatattc tgtttcccga ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt
    21241 catgtttttc tttttcagta ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt
    21301 tttcttcatg aaggtcacgc tgctgctgaa taatttctac taatgccgga tcattcacgt
    21361 ctttgacgaa atacaatttc agaacaaatt cgtctcgatt aaccggtagt tcattggtgg
    21421 gtgtatgaat ccactctgcc aattgttcct tcccttcttc tgttaattca taaactttct
    21481 tttccaattt ttcacctgtg atctctactt gatgctgaat gattccttgc tcttccattt
    21541 ttgcaagttc aggataaatc tgactatgtt ttgcttgcca aaattctcct atttcatttt
    21601 tgaacgattg attcaattca tatcctgtca tcttcttctt acttaataac cctaacaaaa
    21661 tatatcgaag tgtatttttt cttgccatca gttttccctc cttgacaaat ctttaaatcc
    21721 attctatcat aaatgagtaa tcgaaaacat gtagtttatt acatgtttta atttataagg
    21781 agatgcttaa acatgaaaga atttaaagaa ttggaagatt ttattggaac tcactttatc
    21841 tatacgtatg acaatggttg ggaatatgaa tggtatgcca aaaatgacca tactgttgat
    21901 taccgtatcc atggcggaat ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc
    21961 gttaaattga cggacggcgt attcaaaatc acttggacag agccaacagg gacagacgtg
    22021 gcattggact ttatgccaaa tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg
    22081 gttgaagaac atccagaaat cactgttact ttccaaaacg aacatatcgc agaaatggaa
    22141 gcagctcgcg aaaaatatga aacttatcca aaattacttg ttcctgaatt tgctacgatc
    22201 acttatatgg gtgatgcagg tatagacaat gaagatgtta ttagcgaaga accatacgat
    22261 ggaatgccag atgatatccg tagcggaaaa tacttcgatg aaaattataa acgagtaaaa
    22321 taaggttgtg aaagagaggt tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa
    22381 cggaaaaata gttcctcatg ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
    22441 gaacaccgtt tatcaagagg ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta
    22501 gttagaaaat agcttttcat attttctagg ctagtgggct tattaccgag aagcagccgt
    22561 ttgaacaccg tttatcaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac
    22621 aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg
    22681 attgaacacc gtttatcaaa gattgtaaca actgcatatt cacacagtta aattagtgac
    22741 ttcaaaatga aataatgctc ccagcaaaaa attaggctgg gagcattatt tcgttattct
    22801 gttcatcaaa aatcagcgtt ttgattccat ttcatcacaa gttcctcctc atttactgct
    22861 tcatcttttt gctgtgtttg aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat
    22921 tgattttttt gatagactgt caaagtcagt gttttattgg tttcttttat ttttttcatt
    22981 agttgcgtac cgattctttg ttgccggtat tcccttttca caaagattcc tgcgataaac
    23041 tgatcaatca ccccagcaaa accaataatt tcttgatcta tttctgctaa gtaaatcact
    23101 gcatctggta aagctttcct aacagattct acttgattct cccaataagt tgatggaatg
    23161 aattcatgag aatccaagtt agcatgcagc caaatcgata gaacagattc cagttctttg
    23221 gcagacaatt ttgttgttgt gcggatcatt ttttctctcc ttacttattc attgatttta
    23281 aaataaaaac catttttttg aagctctttt acaagttttc gcccctcaaa aaagtatatc
    23341 ttctcttgtt ttacttttct aataccaaaa gaaacagcaa ctttaggatc atcagctgaa
    23401 acgattcgat aagttccacc aggcaattga tccagcttga ctttcccatg atggtcgctt
    23461 tgtactgaaa acggcacacc atttctagta agtgtttttt tcttatgttt caaaatgaat
    23521 gaactaccat ttaccggttt ccctgacgaa tcactaataa taaatgaaaa atcaagcact
    23581 tttttactta atctatactg atagattagt cgtccgataa acactaacag tatgactaca
    23641 gctattatcg ttcctgcaag aataaaccat tgccgcatgg ttctttcctg atttcctttt
    23701 ccttcttctt tttttaccga ttcgttataa ggaatccgat gtcctgtcac tagcaaacga
    23761 tgcgtgttga tcatatacgg cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa
    23821 atcgttagaa cagacgtgtc atctggcagt accacttgta tatttttaac ttgataagcc
    23881 aatttctccc ccaaaacagt caaaataaac gtgtctccgt tttctaaacg atctaattcg
    23941 gtaaataacc gtcgattagg taaaccgctg tgagcagaga tcactgaatg cgtcgaattt
    24001 ccaccaagcg ggaaagaggt tccttgcaag acgcctgctc cattttcaag gatcctacca
    24061 ttgagccgat cataaagtga aatatttaat tggatcttag gtatattgat acttccgatc
    24121 agccattcct ctaattggct ggatgttgta ctttcatttc tgttcttttc attgaaagga
    24181 tcagtaccag gattgaatcc ttggtcagtt aatcgttggt ttttctcctc cattttcttt
    24241 cgctgcactt caatttcagc tgcggtccgt ttttgtactt gttcgatacg tttttgatca
    24301 ataaaactat ttaaagaatc cacataaaat ggatataaag cgatcagtaa ccctacgacg
    24361 atacatatta ataaaaacaa acgaaacaaa agtcgacgag actgtttttg cttactctgc
    24421 ttcacatgtg tcctcccttg caagtttttt tgttctttct tttattatat gggttcagga
    24481 aaaaaataga aaatgaaagc cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa
    24541 gtacagccca aaaaaatagc tactcatatt ttttgttggg ttgggcttat taccgcagat
    24601 gcaagctttt gaacaccgtt taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa
    24661 taagagcgct agtcagaaaa tagcttctca tattttctag gctagtgagc ttattaccga
    24721 gaagcagctg attgaacacc gtttaccgag aggtcatgaa aaagctactt tgtttcgagt
    24781 attaagaaaa attctgtttc aaaacaagga gatgcaacaa aacttatgtc gcatcctctc
    24841 tccttagaac tttcacttat gtttttcttg taaaattcat aaatattttc atgaattttc
    24901 taatttcttg cctacctctc gcataggata tatgatgaat ttatctaaag tttgcaaagg
    24961 agttcgacaa ttgaaaaaat atggcccatt tttagcaatt tttttcattg ctttggcatt
    25021 tctcatgcct catttattca cacataaact aattttgggt gctgatgccc cattccatta
    25081 caatcggttc tatgatgccg cgcaacaaat taaatatggg gatttccatt attttccttc
    25141 cctttatgga ttccagcaaa gtggcaggat cgtgaatgcc gtttacggcc catttttcgc
    25201 ttatttacaa ggattgatcg tgttgcttgc aggtagctgg tttcattatc agattctttc
    25261 aaatttcatt ctttatctta tttcaggatt ttctatgctg attttactga gatatgtcaa
    25321 tgttcgaaat tgggtaagtg taccagtagc cgttttattc atgagtacgt attctattca
    25381 atactggtct atcaaccaag gattcactag ctgggggact tgtttttatc ctttatgtat
    25441 gatccctatt gctgatttcg tccttcataa aaagttccct gttatcaaag tcgctgtctc
    25501 agtgggactt atgacacaga tccatttact gtcttcgatc atgttagctt tgatgtatat
    25561 ccctttttat ctttattact tttttaacca gactgaaaaa ggaaaagcac tgcttcaact
    25621 gattaagtcg gtcttgcttt attttgttct gacagcaaat atttgggcag gattagctat
    25681 tgtttacagt gggaataaga ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc
    25741 gatagatctg gatggcagtt attggctcca ttaccctaag gcctttccta tattgatatt
    25801 atcaggtttt ttgcttttta ttttctttca acaaaagaac accttattcc aaaaaatcgt
    25861 gttgtacaca agtcttgttt tcttttttct ttctactagc ttgattccat ggacgacatt
    25921 gatcgaaaaa aatgtgcctt ttattagttt gatccagttt ccatttcgat ttttcgtgcc
    25981 atttacgcta ttgcttctac tttaccttgc cctttcactg aaccagtggt ctgaacaaaa
    26041 gtggttccgt cttttaagta tatttggcat tcttattttt tcagcacaga cgatccagaa
    26101 cttataccag catctggaaa agtgggaaaa tgaaacattt gtcagtcgac atacttatct
    26161 attcgatacg ccggaagaag caaggaaaac attcttcagc aaagatatgt ctaccagctt
    26221 gttcgttttc caaaaaacca cacctgacta tctgccaatc tataaggaga caaaagacaa
    26281 taaatacgac cggtataacg aagaaatact agaaaacgaa acaactttta taaaaacaca
    26341 tccaggcggt cttttgacaa tcaagtggaa aaacactggg gacaaaactg ttcatatccc
    26401 tgttatcgtc tacgatcgga ctgtgctaca gcaaaatgga aaaacattga ctgattatga
    26461 agtaacagat atcgggaccc caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct
    26521 tcattatcaa acgccaatct atttctattt cattctttct cttactttga ttggctggtt
    26581 taccttgcta tgtttattta tttatcatag atacaagctt ttacgtgctt aggcaaaagg
    26641 gataaataca tccgatcaaa aaactctcca aacggtataa tcgtttggag agttttattt
    26701 taatcagttt ttttatgtta tatgttatat tttttattcg ttcacatcta attcgatttc
    26761 caccccgtag tgatcactga tgattggact atcgatccca tcaaaaatcc gttgatattt
    26821 tttaacctgc attccttcag ggacgaaaat aaaatctata cgcaattttt ctgtattttg
    26881 ttcccaacca tctattttct tttccacagt agctgctttt cctttttcat tcgcgacaac
    26941 atacgaatct tgaagaagaa ggcaactttc tcttacagta taataacctt gagagtctac
    27001 tggattattg aaatcaccta ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc
    27061 caagtttttc cactcaaaat aaaagccact ttttttatct gtccaccaag aaaagtggca
    27121 gctcgagaca aaaatgtcct gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt
    27181 ctgataatct tctttctttt gaaattggga gacggtaagg acctgacttt ccaatgtttt
    27241 tttcgtaagc agtgcatttc cttcttcata tttatcataa ccgatgtggc tcatttccca
    27301 actccaaaaa tactcttttc cgtgttcctt taaaagctga actaaacggt aggcaaaatt
    27361 atctttacgg ataggtactt gattctttac cggacagaac ttcattagct tcgcaggttc
    27421 tacttcttca gattccagta attggttgat ttcttgtaaa gcaatcactt cataatcttc
    27481 taacaggatc ttatctgcta attcttgcaa ttttttctct ggttctggct caagccagct
    27541 atgcgtgttg agtgtcatga atttcattgg ttttctcctt gtcttcattt cgaaacaaaa
    27601 gggttgtgac aaaatttttg tcacaacctc tccagcaaat agtgttctcc tgctaactcc
    27661 tcagtaataa gctcaatgga ccaggagcta ttttttgttt ggctgttttt atcacttggt
    27721 gcagggcagc tttttcacga cctctccact aaacagtatt caaactgctg cttctcggta
    27781 ataagcccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt cttatgactt
    27841 gatgcaggac agctttttca caacctctcc ttacatttta atggagccga tcactttacc
    27901 agctgtcact gtacctaatt gcttgatcac tacttcttct actttatcgt ttgtaaatac
    27961 gatcatgatc gttggatctt tttcttcgtt ttggatcaca tcaagatcca tctcagctaa
    28021 tttcgttcct gttcctactt cttggccttc tttgacagca atctcaaaag ctggctgatt
    28081 catttggact gtatcgattc ccatatggat caaaacttcg ataccatctt tcgtttccag
    28141 cccaattgca tgtttgctag ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac
    28201 ttctcttgat gttggaataa cagcaaagcc gtcacccatc attttttgtg agaataccgg
    28261 atcttttact tcagataaag gaatcacttc tccagaagct gcggaaacta gttcttgttc
    28321 tatacctaag tattgcttac tacctgcttg tccatttcct gtagaaacca tttctggctc
    28381 tggaatctct gctcctgatt gcagcagatc ttcgatatct gatttcaaga catctgcttt
    28441 tggtccataa actgcttgga ctccattgtc tttgacgatc aatcccatcg cccctgctcg
    28501 tttccaggct tcttcagtcc ctactttttc tcgatcattt acacttacgc gtaaccgagt
    28561 catacaagca tcgacatctt tgatattttc ttttcctccc agcaaatgaa tgatctggac
    28621 cacttgctga tctgctgttc ctgttccgct gttagataca acggtttctt cactgtcatt
    28681 gtcgtaattt ccgttacgtc ccggtgtagc aaagttgaat tttttgatca agaaatttgc
    28741 taagaaatac gtaacaacac caaaagcgat cacacacaac acgaagttga taagatctcc
    28801 acctagaccc gctttgatag ctaacggtgt ccgagtcagc aattcgatat tcccaaatga
    28861 atggacccgc aatggcaaga tatctgccat cgcaaatgca gccccttgga tcactgaata
    28921 aatgacatat aatggcacag cagcaaacat aaacatgaac tccaatggtt ctgttacacc
    28981 tgttagaaag acagccaacg ctgctgaaaa atacattgat ttatatcttg ctttcttatc
    29041 aggatcgaca ttacgataca tcgcaaaagc cattcccatc aagatacctg aagaaccaat
    29101 catttgtcct actttgaaac gagccggcgt ccagttatgc aacacaaact catattgcga
    29161 catatcccct gcacctttaa gattcactaa atcagtggcc caagccaacc ataacggatc
    29221 ttgcccaaat acttgtgtcc ccgcctgtgc gcctgataaa atctcatacg atccgccaag
    29281 ctgtgtatag ttgataggaa tcgtcagcat atgatgcaat ccaaatggca atagtaaacg
    29341 ttctaatgtt ccaaataaaa acggtgctag aatcggtgcg ctttcttgtg attctgcaat
    29401 ccataaacca aaattattga ttccagcctg tacgtttggc cacaccacag caagaatcaa
    29461 tgccacgatt gttgaccata agataacaac aaacggaaca aagcgctttc cattgaaaaa
    29521 tgacaaagca tccggcaatt ttctataatt atagtactta ttataagcca ttgccccgac
    29581 aaatccagcg atgatcccta cgaatactcc catattcaaa gctggtgctt ccaatacact
    29641 tgtaaaaaat cctttaacca tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc
    29701 attcgcaagc atgtcacttg tgacaccaaa gatagcccca gtgatccgat tgattaaaat
    29761 aaatgagatc ccagctgcaa atgctccgcc tgctctttct tttgcccaac ttcctcctat
    29821 tgctaaagca aataaaatat gaagattacc aataattgcc caaccaatat tttcaattac
    29881 tccgcccgtt gtgacaagcg gcgtccaatc aggattgatc aagggaattg atttccctat
    29941 actgatcatc aacccagctg ccggcataac tgctaccaca accattaaag cctttccaaa
    30001 tttttgccaa aactcaaaac taaatatctt tttcattccg tttagctcct ttacgcaatc
    30061 ggtttcgtta aacgtattat agcgtgcgtt ttcttttttg taaatgcttt attttttatt
    30121 tttctgtcta taccattcta taaaacctat aatacgtgtg ttcccttcta taattttctc
    30181 ctaaaaattt atttttttaa gcttttttct atgaaatatt ttcttttgtc tattgacttg
    30241 tgcaatcggt tgcgtttatt atgaacgtat aaaacagctc attaggagga gaaaaatgaa
    30301 acagatcaaa cgactatttg ctattgatcc atggaaaatc agcacgacac aattagataa
    30361 aaaaaatctt cgactgcaag aatctttaac aagtattgga aacggttata tggggatgcg
    30421 cgggaacttt gaagaaactt actcagggga ccaccatcaa ggtacttatt tggcgggtgt
    30481 ctggtatccc gataagacac gtgtcggctg gtggaaaaat ggttatccag aatactttgg
    30541 aaaagtgatc aacgccttga actttatcgc gatagatatt tcgatcaacg atcagctgat
    30601 cgacttggct tctttggaac cagaagactt ttactgggaa ttagatatga aaaatggtgt
    30661 attatctcgt acattcacaa tcactacttc tactaataaa gtacgcattt ccttcgaacg
    30721 ttttctgagc attgtcaaaa aagaatcagc ttatatctat ttaactatag aaatgcttga
    30781 aggtagcgga acagtcaaac tcgtatcaaa attggatgga aatgttcaaa acgaagacag
    30841 caactatgat gaacattttt ggaatgagat tgatcgtgga actatagaag aaatcggcta
    30901 tttgacgact aaaactattc caaacaactt tgggattgaa caatttactg ttactgcagc
    30961 aatgcgccat ttcgttaatg gaaaatcagt catccctgac tatcacgaag atactttagc
    31021 attacatggt tgcttgtctg cagaattatc tcaaggtgag acatggtcag tggaaaaaga
    31081 aattatcgtt cttactagta gagatattcc agaagcgcag caagcagaaa aagctgtctc
    31141 agaattaacg aaccagacaa acaattatat ccaagcaaaa gaagagcaaa cagcggcttg
    31201 ggcagaacga tggaagcttg ccgatgtggt catcgaagga gacgaagaag cacaacaagg
    31261 tattcgattc aatttgttcc aactattctc tacttactac ggcgaagatg aacgattgaa
    31321 tatcggcccc aaaggattca caggagaaaa atacggcgga gccacttact gggatacaga
    31381 agcgtatgct gtccctcttt atcttgcttt agccaaacct gaagtaacta agaatctgct
    31441 gaaatatcgg cataatcaac tgccgcaagc aatccacaac gctcagcaac aaggattaaa
    31501 aggcgcgctt tatcctatgg tcaccttcac tggtgtcgaa tgccataacg agtgggaaat
    31561 cacattcgag gagattcacc gaaatggcgc gattgcctat gcaatctaca attatacgaa
    31621 ttacaccggc gatacggaat atttgaaaaa tgaaggactg gaagttttag cagaaatcgc
    31681 acgcttctgg gcagaccgtg tccatttttc aaaacgagca aataaataca tgatccacgg
    31741 tgtgaccggc ccaaacgaat atgagaacaa tatcaataac aattggtata caaatacgat
    31801 cgcagcttgg gtcttgcgtt acacgcgaga aaattattta acttatcaaa aagaagctgc
    31861 tatcactatc agcgaagaag aactagccaa atggaaagat atcgcagaga acatgtactt
    31921 cccagttgac gaagaattag gaatctttgt ccagcatgac acattcttag ataaagatct
    31981 catgcctgta tccgatattc ccacaagcga tcttcctctg aatcagcatt ggtcttggga
    32041 caaaatcctt agatcttgct tcatcaagca ggcagatgta ctacagggga tttatttctt
    32101 caatgatact tttacaaaag aagaaaagca aaaaaacttt gatttctatg aaccaatgac
    32161 tgtccatgag tcttcccttt cgccaagtat ccattcgatt ttagcagcag aattaggaat
    32221 ggaaagcaaa gctgtagaaa tgtaccagcg aacagctcgc ttggatttgg ataattacaa
    32281 taacgacaca gaagacggct tgcacatcac ttccatgact ggtagctggt tgtcgattgt
    32341 ccaaggattt gctcaaatga aaactgccca tgatcagttg agattcgcac catttctgcc
    32401 aaaaacttgg aaagcttatt ctttccatat caattatcgc ggacgtttat tgttcatctc
    32461 agtcacagat gaacaagtta ctttcgagtt attagaaggt tctcctctag aaatttacgt
    32521 ctatgaacag tcttattctt tagcggatcg tctagaaata acgctaagaa aggaggaagc
    32581 aaatgtttaa aggggtattg tttgatttag acggtgtcat taccgatact gctgaattcc
    32641 attatcgtgc ttggcgaaag cttggtcagg aaatcggcat tacgatcgac cacacattca
    32701 acgaacagct aaaaggcgtc agccgagaag actcactctc tttgctttta gcttatggcg
    32761 gaaaagaaca ctctttttca aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt
    32821 attatttaga gatgatccag acgatcgaac caaaagatgt cttccccggg gtcgtgcctt
    32881 tactggattc tttaaaagaa gaaaaaatca agattgctct tgcttctgca agtaaaaacg
    32941 ggccattcct acttgaaaaa atgggtctta caccttactt tgatgctatt gccgacccgg
    33001 cagaagcaac aaacggaaaa cctgcgccag atatcttcct ccttgcagca aaagctgtcg
    33061 gactaaaagc agaagaatgt atcgggatcg aagacgcgca agccggtatc caagccatcc
    33121 tctcaagtgg cgctcaacca attggcgtgg gacgtgctga agaccttgga gaggagatcc
    33181 cgcttgtccc tgatacgact tttcttacga tcgattactt gaaagaaaag tggcatgacc
    33241 atggataatc cagagatctc cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca
    33301 ttgaagaacg actacttaga agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc
    33361 ttcgcaccag acaaagaagg aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa
    33421 gatgttctaa cagacaaaag cttttttgga gcaactgtcg gacctgtagc cggaaggatc
    33481 cgggatgctt cttggggtga gcatagattg gagaaaaact gcggatcaca ccatatccac
    33541 ggtggcacga aaggctggtc tttccaattt tgggaagtag aagttttcaa aactccatca
    33601 tcaataggtg tggtctttta cttaaaagat gaattttcta cctatccagg accaatcact
    33661 gctacgatca cttaccgttt aacaaaaaac gaattagaga tgacaacctc tgctagtagc
    33721 cttgttgaaa ccatctgcaa tccgaccaac catgcctact tcaacctttc aggaaatgga
    33781 acaagagaca tctatgaaca tcagttaacg gtctttttag atggtgtgct tgaattagat
    33841 caagaaaaac tgcctactgg aaattggaca aaaaaagaaa aactgccaat cgactttaga
    33901 aaatccccaa cactgcaaga gatccttgca tgttatccag atggactaga tgatgtattt
    33961 cttttgcatc atcctaggct ttccaaaact tccttacaat tattcgagaa gcattctggt
    34021 cgccaaatga ctatagcaac ttctaataaa agtatggtat tattttctac gactggattc
    34081 gaagctgatt tctctgtcaa tggaaaacag atgcattcaa actacggatt agcaatcgaa
    34141 ccgcaagaaa tccctgatat cgttcatttc cccaaatttg gatcgatcaa cctccatcct
    34201 ggtcaagaac ggatcagtca gacgatttat cggttctctg cacaataaca agagaaaggt
    34261 gtttttatca tggctattac cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg
    34321 accgtctctc gtgtcatcaa cgatcatcct agtatttccg agacaacaaa gaagaaagtc
    34381 cgaaaaatta tggatgaact aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa
    34441 cgcatttcca gtgccatcgg cgtaatctta ccccctcttg attcaaaaga gcggatcggt
    34501 aacccatttt atcttgagat catggaagca atcaacgaag aagcgcggaa ttatgatatg
    34561 actacagcaa tcgctactgc taaaaatttt gaattgctgt tagagaacgt ccagcgtatg
    34621 catttacaaa aacaagtaga tggttttatt ctagtgtatt cagatagtca agatactgtc
    34681 attgattatt tataccaaaa ccacatccct ttcactttga tcggacagcc ttatcatcat
    34741 gagaacgaaa tcgtttatgt agataatgat aaccagcttc taggtaaaca agcaacagaa
    34801 tttttgatcc agaatggaca taagaatatc ctgtttgtga caaacacgac tcatgaaagt
    34861 ttgtatttcg aacgatattt cggttatcaa aaagcaatga tgatggctgg tcttcctgcg
    34921 ttatccagtg tagtactaga gcatccggag gattatctgt cctttgagaa aacgttgaaa
    34981 gaaaacgaag ccacagcatt ggtggtcatt gacgatatct tcgcccttcg cacgatgcag
    35041 ttggcgcaaa tgtatggcta tgtcattcca gagacactct ccgtcattag cttcaacaac
    35101 tcgatcttct caacattaac acacccttac cttacaagta tcgatatcga tatctccgaa
    35161 ttaggacgtg tcgctatgca aaagctgcgc gaattgatcc atgaagaatt gagcagtggc
    35221 gtacgtttgg tcatccctca tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac
    35281 ccatactaaa tagctgactc actaaatacg ctgaccattt atcctatggt cagcttttta
    35341 tgtaaaagaa gatactactt ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa
    35401 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
    35461 tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt gagtttcttc
    35521 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt caatttttta
    35581 cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca agaatcgaga
    35641 aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata atgaatgtgc
    35701 cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct agtgttcgtg
    35761 tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa
    35821 gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag ctcaaggcag
    35881 taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga acgatggtat
    35941 caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa atcaaactag
    36001 cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc tttggatcag
    36061 gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt
    36121 tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat gtagggatag
    36181 ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat gtccaaggag
    36241 agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct agatcggctg
    36301 aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat gctgggaaat
    36361 ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata cccgctgcca
    36421 agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg gaaacgactg
    36481 cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc
    36541 caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt gcacaaacaa
    36601 gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa accataatcg
    36661 tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa agaaaaatca
    36721 ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc tcatcccatg
    36781 tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg atcgggatat
    36841 gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac acgatcaata
    36901 cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta tttcaaatga
    36961 aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa tcagcgacaa
    37021 aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct acctcaatcc
    37081 cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg accactgtga
    37141 tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca aatacgacaa
    37201 cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt ccgtttttac
    37261 caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc aatgaaacag
    37321 caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt gtcaagaata
    37381 cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata acgattgatt
    37441 gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat ccgaattttg
    37501 gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca gtaacccaag
    37561 ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt ccgatcactt
    37621 ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt gctaattcca
    37681 tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc aataaaacta
    37741 atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct ttcaaacgag
    37801 gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca gctaagtatt
    37861 tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag tcacctagat
    37921 agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg aacatgatga
    37981 ctactgggat atatacccct agccatacac cgatgttccc gccgactttt gccatatcga
    38041 atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct aaaacgaacc
    38101 agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt cctaaagtag
    38161 aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca acgacaaatc
    38221 cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct tgaggaagca
    38281 tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa actgcaaaaa
    38341 ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc cgaaccgttg
    38401 cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt acttcagatt
    38461 tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct ttgaagtagt
    38521 ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag tttattgctt
    38581 gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag gtctcccctt
    38641 gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt tctaacatga
    38701 ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt tttccaattt
    38761 ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt ctttgttcat
    38821 gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt tccattcttc
    38881 atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt ctgggatagc
    38941 aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca cgtatgaaga
    39001 ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt tgcctttttc
    39061 tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa cttcgatttc
    39121 tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat gtgcaccttc
    39181 gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga catggtgtgc
    39241 tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat aagccccaag
    39301 taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag agataacgtc
    39361 tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat agcccatttt
    39421 atgtgggtca atcgttacag attctgcttc ttcgattgct ttatatgcac tgtgtacttc
    39481 ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa catcttttaa
    39541 atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc
    39601 ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata cgcgacgcaa
    39661 tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta caacaccgac
    39721 tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt caagttcgtt
    39781 gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta aaccgatacc
    39841 gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca accattttcc
    39901 taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt cgattttttc
    39961 tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta attcccaatc
    40021 gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca atgatttgat
    40081 gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc
    40141 ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt cttccatttg
    40201 gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca tagcaaaatt
    40261 gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt aacgtccggc
    40321 attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata gaacatcctt
    40381 tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg taataatagg
    40441 catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca attcattcaa
    40501 caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg catttaggtt
    40561 tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata tttaaatttt
    40621 gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg cctgattttt
    40681 aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa taattaaaaa
    40741 aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg acaaccagcg
    40801 ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc tttttcaagg
    40861 taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt cttacctttt
    40921 cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa atttaaatcg
    40981 gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg tgcttgacgt
    41041 ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg tgtgcttgaa
    41101 atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt tagttcttta
    41161 atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta
    41221 tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct
    41281 gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt aaagaatttc
    41341 aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa ctcaaatggt
    41401 gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa tttcgttccg
    41461 tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat
    41521 tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc accgatttgc
    41581 aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag gatttggtaa
    41641 gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc ttttgctaac
    41701 atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa agagattttt
    41761 gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa caattttctc
    41821 atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg caacacacgt
    41881 tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat cagaatatat
    41941 ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa atgtccgata
    42001 tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt ttcttcaacg
    42061 agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca agttaattca
    42121 tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag gaacctgcta
    42181 gcatgtgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt cccttggcat
    42241 ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga tatccaacat
    42301 acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa
    42361 ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac attggcttta
    42421 acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat catagagcgc
    42481 aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt ttttcttttt
    42541 tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa ataacttttt
    42601 ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat tctttcttcg
    42661 ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa
    42721 aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt ttaattctaa
    42781 gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa
    42841 taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact caagcaaaaa
    42901 atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa gatagtaaga
    42961 acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa gaacaactga
    43021 tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg atcgtggacc
    43081 cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg gtgactgttg
    43141 aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta gttaatttga
    43201 atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt gcgctacctg
    43261 aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa tatatggata
    43321 attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa aatgtctata
    43381 aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc gatgcctttg
    43441 tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt
    43501 acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat gaaaaaacaa
    43561 aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac ggctggagct
    43621 ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa gatgtaaaaa
    43681 aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca aatattctga
    43741 tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa aatcaaaaat
    43801 ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct tctacaccag
    43861 cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc gatggcattc
    43921 gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc aatgtccatg
    43981 atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa
    44041 agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc ttcttcggtg
    44101 cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt tttgcgtata
    44161 tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag aactcatccg
    44221 aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca agcatgatac
    44281 tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt tctttttttg
    44341 tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga taacggattt
    44401 tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca atcaacgctt
    44461 tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc agcccttcct
    44521 tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca gctaccttat
    44581 ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa
    44641 tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga gcctcccgta
    44701 attttaaact tttataatat atatagttca aatttattct gaggtttatt ataactatct
    44761 attgagaaag tatcaagcta gcaattcctt gtttatgaat tcattgacag aaatttataa
    44821 aaagaatatt atgtactaaa ataataaaaa aagttttata aaaaggtata aaaccttttt
    44881 attaaactat ttaatcttgt ttagtatata tttttagaaa aaggagtggg gtttttatgg
    44941 cagaaatcgt caaagtcaca catttgaaaa agagctttgg tcgtttcgaa gctttaaaag
    45001 atgtctcttt caccgtacat tcaggtgaag tcgttggatt tatcggacca aatggttctg
    45061 gaaaatccac gacgattcgg acacttttag ggattataaa aaaagacggc ggaacggcaa
    45121 cgatttttgg gcaagatgta tgggaagata gtttatctat ccatgaacgt ctttcttatg
    45181 ttccaggcga tatctctttg tggggaaatt tgactggagg agcgatcatc gatctcttca
    45241 tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga actgattcaa cgtttctctc
    45301 ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga
    45361 ttgctgcttt ggctgttgat tcggatctct atttatttga tgagcctact tctggtttag
    45421 atcctttgat ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa cagggcaaat
    45481 ccatcctact ttcttctcat attctcagtg aagtcgagcg actagcggat aaagtcgtga
    45541 tcatacgcga aggaacactc gatgaagaag gaacactcga tgaattgcgt catttgactc
    45601 gttcgaccat cactatagaa acagaagcag agaatagtca cttagcacaa ttgaacggtg
    45661 tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac gaagctttcg
    45721 atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa atttgaatct attccaccga
    45781 ctcttgagga tttattcatg agacattatg aaggatgagg aggaagcgaa atgactgata
    45841 aattttcacg ctggtccacc cttttttctc aaaaccttag ccgtgactgg aaaaagatcc
    45901 tgatatggct agtcggtgtc actgcttttt ctggtggttt tgtccctgcg tttgaagaat
    45961 tgacttccgg acaaggatta gctggattat acgaaacctt gcaaaacccg gcaatgattg
    46021 caatggtcgg accaactcca gcttcgaatg ctgctgagta tactttagga gcgatgtatg
    46081 caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg
    46141 tgagccaaac gagaaaaaaa gaagaacttg gtctgattga acttattcgt tcctttcgag
    46201 tcggacgtca agcaaactct ttagctgtca tcgctgaaag tattctgatc aatttgcttt
    46261 tagcaatatc gatcagttgt acgatgattt ttttccaagc ggattcgatc actgcagaag
    46321 gtgcttggct ttttgggcta tctgtaggta gtatggggat tttggggagt gccgttgcac
    46381 tgtttgctgc acaactgatg ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt
    46441 taggtggact ttaccttcta cgagcaataa cagatgtagc tgattcctcc ctttcgatgt
    46501 ggaatccttt aggctggtct tatttgacct atccatttac aactaacaac tggattcctt
    46561 tgctttattt gtttttgttc agtctaatct tgataatcag ttcatttgtt ttagaggaac
    46621 atcgagattt gaacaccggc tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt
    46681 ctcttttatc cttgcgtggt ttattattct atctaaataa agggatgaca atcagttggc
    46741 ttatcacttt tggtttgatg ggggcggcat acggctctat ctacgggaat atgcaacagt
    46801 ttttagaaag taacgactta atgaaacaga tgtttatcca atctggcgtc tcaattgaaa
    46861 aaaactttac aagcacgatc atggtcgtct tgatttgtct agctgctatt ttaccaattg
    46921 ctagcatcaa tcgtttattt acagaagaaa cccacggacg tatgaatcag ctgttcgcaa
    46981 caaaaatttc taggaaccaa ctttattgga ccaatatgtt actagctatt ctatttgcta
    47041 tattggcaat cctgattagt tctggtgcac tagggctttc tgctttggct tcgatgaagg
    47101 atgcgaaaga catggatcta ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg
    47161 ttttagtgtt tatcggaatt agtggctttc tattcggatg gcttcctcga atcggtaaac
    47221 ttgcttatat ctacttagct tactcctttg cactcaatta ctttggcgga attttggatt
    47281 taccagaatg gttttccaaa acagctgtcc ctagctgggt tccacggatg ccaaacgatt
    47341 cttttgatct ttccgtgttc cttacgttat catgtatcag ccttgcactg ttcgtccttg
    47401 gttctattgg ttataggcga agagacttac tagaaggata ataaaaaagc atgtacaatt
    47461 tcatgctagc ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt tttttattta
    47521 caagtcttct aactgctctt tatcaatgat ccagacttct cgattctttc tttttacata
    47581 accattctcc tctaataatt taaattttct agataaagtt tcgggggtcg taccgataaa
    47641 tgcagcaagt tctttcattt tcattggcag gaaaaagtga tccttccctg tcgctttagc
    47701 taagtcaagc agatagacag cgactcgttc ttctactttt tccatcatca aaaaagttgt
    47761 ctgctgctgt acttttgtta atttctcaac gttgattgct aaaagtttca gactaagggc
    47821 gggataatgc agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt
    47881 ttctagtgct tcgccaaata aagactcatt tggaacgcct agcaactggc tctctccctc
    47941 atatccgccc ggctctatta ctcgtaaaag ctgttccttt ccgctagcag ataattgata
    48001 cactttcata ctgccggagg ccacgatcac taattgaggt tctccattcg gcgcaagaat
    48061 ctgttctcct ttttccacat agtgatgttt gacaagtgcg tgtacttttt cttgatcttg
    48121 ctgtactaag tgattgaaca aaggaacaaa tgaaacacat aaatgatcat gactcataat
    48181 ctctcctcct tctaaacagc tatttctaat aaagaaacat aacttccctt attccaactg
    48241 ataattttct tttatcatag cataaacgtc aagcgaacca gataccagca aatagagtgg
    48301 atttcttttt gtcccttgct ttattttcct tcttttgaga ccttaagtaa ttgattccta
    48361 tcaatttttt aaatatttac tttttgaata atcaaaaaca aaccttcact cgcaagccga
    48421 gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt tttccgcacc
    48481 tagcttttga tatagtggtg ctagttcttg taaggtttca gcggttattt gctcaacgtg
    48541 ttcgatttct tgcggataga agaatagttt acccatttga aaggttcggt ctctcatcgc
    48601 ttttttcaac tcttcttgtt tctttgtatc ggacaaatag tcagacaaag ctaagtggtc
    48661 gtcaagttca tgttctggtt gcggccatat gtaataatcc ttgatatcga ctgttttgct
    48721 ccactctttc agtatcgcaa gcaactggtt atacgctgca atgctaccat atcgttcttc
    48781 gcttttataa tgttggtaca tcaaatagat ttggtattgt ttttgattca acaaaactgc
    48841 tagacaggca ttctcgtctt gacgagattc gcttcggtac gcgctccaaa aatgactgcg
    48901 taaattccag ccattcgtcc agctttcgat tttaggtttg ctcatgccgt acatatcagg
    48961 caaaagtgca gctgtctgga gctgaatctc tttccatttt tgccagtgtt ctttatattc
    49021 tttcttgatt tgttcaatct tatctgttgg atggctctct ttgagctgtt tgaatgcgta
    49081 gattggtcga tcaaacaact gataatctga ttttccaagt tccattgttt cgcctctttc
    49141 cgttatcttt tgtattctac ctgaattttg cgatatggac aaatagaggt tgtgaaaaag
    49201 ctgttctgca tcaagtcata agaacagcca aacaaaaaac tgcgatgagc atcaccgcag
    49261 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
    49321 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
    49381 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
    49441 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
    49501 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
    49561 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
    49621 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
    49681 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
    49741 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
    49801 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
    49861 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
    49921 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
    49981 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
    50041 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
    50101 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
    50161 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
    50221 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
    50281 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
    50341 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
    50401 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
    50461 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
    50521 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
    50581 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
    50641 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
    50701 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
    50761 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
    50821 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
    50881 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
    50941 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
    51001 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
    51061 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
    51121 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
    51181 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
    51241 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
    51301 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
    51361 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
    51421 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
    51481 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
    51541 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
    51601 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
    51661 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
    51721 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
    51781 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
    51841 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
    51901 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
    51961 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
    52021 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
    52081 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
    52141 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
    52201 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
    52261 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
    52321 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
    52381 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
    52441 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
    52501 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
    52561 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
    52621 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
    52681 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
    52741 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
    52801 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
    52861 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
    52921 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
    52981 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
    53041 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
    53101 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
    53161 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
    53221 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
    53281 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
    53341 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
    53401 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
    53461 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
    53521 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
    53581 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
    53641 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
    53701 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
    53761 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
    53821 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
    53881 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
    53941 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
    54001 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
    54061 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
    54121 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
    54181 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
    54241 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
    54301 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
    54361 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
    54421 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
    54481 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
    54541 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
    54601 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
    54661 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
    54721 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
    54781 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
    54841 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
    54901 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
    54961 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
    55021 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
    55081 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
    55141 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
    55201 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
    55261 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
    55321 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
    55381 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
    55441 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
    55501 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
    55561 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
    55621 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
    55681 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
    55741 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
    55801 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
    55861 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
    55921 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
    55981 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
    56041 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
    56101 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
    56161 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
    56221 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
    56281 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
    56341 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
    56401 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
    56461 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
    56521 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
    56581 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
    56641 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
    56701 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
    56761 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
    56821 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
    56881 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
    56941 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
    57001 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
    57061 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
    57121 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
    57181 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
    57241 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
    57301 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
    57361 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
    57421 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
    57481 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
    57541 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
    57601 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
    57661 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
    57721 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
    57781 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
    57841 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
    57901 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
    57961 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
    58021 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
    58081 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
    58141 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
    58201 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
    58261 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
    58321 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
    58381 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
    58441 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
    58501 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
    58561 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
    58621 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
    58681 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
    58741 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
    58801 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
    58861 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
    58921 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
    58981 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
    59041 acctcatggg gtatattaaa caggagcttt attaatatgt caaccaaaat gactcttcat
    59101 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
    59161 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
    59221 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    59281 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    59341 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    59401 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    59461 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    59521 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    59581 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    59641 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    59701 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    59761 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    59821 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    59881 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    59941 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    60001 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    60061 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    60121 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    60181 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    60241 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    60301 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    60361 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    60421 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    60481 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    60541 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    60601 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    60661 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    60721 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    60781 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    60841 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    60901 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    60961 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    61021 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    61081 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    61141 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    61201 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    61261 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    61321 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    61381 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    61441 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    61501 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    61561 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    61621 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    61681 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    61741 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    61801 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    61861 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    61921 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    61981 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    62041 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    62101 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    62161 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    62221 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    62281 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    62341 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    62401 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    62461 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    62521 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    62581 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    62641 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    62701 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    62761 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    62821 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    62881 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    62941 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    63001 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    63061 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    63121 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    63181 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    63241 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    63301 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    63361 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    63421 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    63481 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    63541 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    63601 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    63661 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    63721 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    63781 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    63841 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    63901 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    63961 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    64021 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    64081 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    64141 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    64201 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    64261 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    64321 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    64381 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    64441 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    64501 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    64561 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    64621 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    64681 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    64741 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    64801 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    64861 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    64921 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    64981 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    65041 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    65101 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    65161 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    65221 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    65281 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    65341 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    65401 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    65461 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    65521 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    65581 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    65641 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    65701 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    65761 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    65821 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    65881 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    65941 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    66001 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    66061 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    66121 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    66181 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    66241 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    66301 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    66361 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    66421 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    66481 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    66541 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    66601 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    66661 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    66721 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    66781 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    66841 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    66901 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    66961 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    67021 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    67081 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    67141 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    67201 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    67261 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    67321 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    67381 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    67441 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    67501 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    67561 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    67621 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    67681 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    67741 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    67801 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    67861 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    67921 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    67981 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    68041 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    68101 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    68161 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    68221 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    68281 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    68341 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    68401 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    68461 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    68521 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    68581 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    68641 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    68701 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    68761 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    68821 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    68881 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    68941 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    69001 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    69061 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    69121 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    69181 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    69241 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    69301 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    69361 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    69421 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    69481 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    69541 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    69601 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    69661 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    69721 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    69781 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    69841 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    69901 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    69961 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    70021 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    70081 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    70141 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    70201 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    70261 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    70321 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    70381 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    70441 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    70501 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    70561 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    70621 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    70681 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    70741 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    70801 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    70861 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    70921 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    70981 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    71041 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    71101 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    71161 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    71221 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    71281 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    71341 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    71401 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttctt caaaaggttt
    71461 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    71521 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    71581 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    71641 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    71701 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    71761 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    71821 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    71881 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    71941 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    72001 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    72061 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    72121 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    72181 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    72241 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    72301 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    72361 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    72421 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    72481 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    72541 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    72601 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    72661 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    72721 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    72781 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    72841 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    72901 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    72961 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    73021 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    73081 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    73141 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    73201 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    73261 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    73321 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    73381 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    73441 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    73501 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    73561 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    73621 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    73681 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    73741 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    73801 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    73861 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    73921 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    73981 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    74041 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    74101 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    74161 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    74221 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    74281 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    74341 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    74401 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    74461 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    74521 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    74581 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    74641 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    74701 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    74761 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    74821 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    74881 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    74941 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    75001 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    75061 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    75121 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    75181 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    75241 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    75301 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    75361 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    75421 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    75481 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    75541 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    75601 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    75661 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    75721 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    75781 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    75841 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    75901 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    75961 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    76021 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    76081 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    76141 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    76201 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    76261 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    76321 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    76381 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    76441 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    76501 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    76561 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    76621 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    76681 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    76741 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    76801 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    76861 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    76921 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    76981 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    77041 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    77101 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    77161 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    77221 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    77281 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    77341 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    77401 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    77461 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    77521 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    77581 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    77641 aagcgctttc atctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    77701 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    77761 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    77821 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    77881 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    77941 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    78001 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    78061 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    78121 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    78181 gcatgatatt ggaaaaaatt gtactgattt gttgaggcaa ccttgcgtgt tggatcaccg
    78241 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    78301 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    78361 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    78421 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    78481 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    78541 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    78601 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    78661 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    78721 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    78781 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    78841 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    78901 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    78961 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    79021 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    79081 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    79141 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    79201 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    79261 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    79321 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    79381 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    79441 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    79501 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    79561 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    79621 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    79681 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    79741 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    79801 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    79861 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    79921 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    79981 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    80041 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    80101 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    80161 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    80221 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    80281 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    80341 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    80401 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    80461 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    80521 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    80581 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    80641 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    80701 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    80761 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    80821 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    80881 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    80941 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    81001 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    81061 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    81121 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    81181 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    81241 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    81301 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    81361 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    81421 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    81481 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    81541 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    81601 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    81661 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    81721 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    81781 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    81841 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    81901 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    81961 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    82021 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    82081 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    82141 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    82201 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    82261 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    82321 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    82381 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    82441 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    82501 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    82561 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    82621 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    82681 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    82741 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    82801 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    82861 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    82921 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    82981 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    83041 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    83101 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    83161 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    83221 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    83281 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    83341 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    83401 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    83461 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    83521 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    83581 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    83641 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    83701 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    83761 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    83821 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    83881 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    83941 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    84001 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    84061 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    84121 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    84181 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    84241 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    84301 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    84361 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    84421 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    84481 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    84541 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    84601 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    84661 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    84721 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    84781 tttacaaaaa aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc
    84841 tgcttctgat tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc
    84901 gattttttcc ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat
    84961 ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc
    85021 acgccagttg agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg
    85081 aagtgccgga gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt
    85141 tcctaaatgc agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa
    85201 agacaaggaa atactattat agatatctac aagcggattg attgggaaaa attcccgttc
    85261 ttgatttgca cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa
    85321 tgtggtaaat gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt
    85381 aaggtaattt cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact
    85441 ttcttttgtt acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg
    85501 aaatacttcc cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat
    85561 tagaataatt gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca
    85621 tgttcgctgt atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt
    85681 tcccaaacac cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta
    85741 tctagtaaaa atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg
    85801 atttctaaaa aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg
    85861 taacccgcag gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc
    85921 tcttctgtaa gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt
    85981 gaattatctt ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct
    86041 acatctgaag gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag
    86101 atccttttat tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta
    86161 tttaaaagat attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca
    86221 ttggagtagt tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga
    86281 tagagtttct tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat
    86341 tctaatttct tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt
    86401 ttttcttgga tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt
    86461 gttaaatcga cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt
    86521 ccgatgtctc gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt
    86581 tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat
    86641 aactagtaaa gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt
    86701 ttttgttttc taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca
    86761 actcttctat actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct
    86821 cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat
    86881 ctttttgttt attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg
    86941 tttcttcata gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc
    87001 caccaggaag tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt
    87061 ttttgatata ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga
    87121 gcgcttttat ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg
    87181 tccggatctc ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt
    87241 gagaaagtgc gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt
    87301 ttttagcaaa tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata
    87361 aatataatga aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt
    87421 cgacttctct ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc
    87481 ccaccaaacg aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag
    87541 gacagctttt tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa
    87601 gtcacaatat tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga
    87661 gctgaacgac aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat
    87721 taagccattt ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg
    87781 agtgtaatcc acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa
    87841 tttgtcatat tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt
    87901 ccctttcgtt cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa
    87961 atgacgatct gattcacgat cacgtttgta ccgacgttgc cataacactt caaagtctgc
    88021 tgttaggcga atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc
    88081 tttttgctta tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata
    88141 caaatcaagt gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc
    88201 ttccaaattg ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat
    88261 ggcatacata tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc
    88321 aggactacca gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg
    88381 gactttccaa gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat
    88441 ttgctggaat cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt
    88501 tagctacttt cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt
    88561 tattgatcaa ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg
    88621 ggacatcaac tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt
    88681 tgaattgtcc atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta
    88741 caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc
    88801 cttcttctgt accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg
    88861 catattctac tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat
    88921 catacatgac agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg
    88981 cgtggtctaa gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat
    89041 tgactaacac atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg
    89101 gcgaacccat ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat
    89161 taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt tgttacttct ttcaatgttg
    89221 tatacatctt ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt
    89281 gcaatttgtc gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca
    89341 gcacgagcag catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct
    89401 ccgatcgtct gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca
    89461 tacaattcag aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct
    89521 tgggtagtgg aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct
    89581 tctggcgata gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttt
//
LOCUS       DACDVT010000003        66000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDVT010000003 DACDVT010000000
VERSION     DACDVT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.003"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..972)
                     /gene="murB"
                     /locus_tag="IWL52_000180"
     CDS             complement(37..972)
                     /gene="murB"
                     /locus_tag="IWL52_000180"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9776775.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1045..1965)
                     /locus_tag="IWL52_000181"
     CDS             complement(1045..1965)
                     /locus_tag="IWL52_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9776776.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..2830
                     /gene="xth"
                     /locus_tag="IWL52_000182"
     CDS             2078..2830
                     /gene="xth"
                     /locus_tag="IWL52_000182"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9776777.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3694..4227
                     /locus_tag="IWL52_000183"
     CDS             3694..4227
                     /locus_tag="IWL52_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9776778.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4717..5241)
                     /locus_tag="IWL52_000184"
     CDS             complement(4717..5241)
                     /locus_tag="IWL52_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9776779.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5234..5707)
                     /gene="tsaE"
                     /locus_tag="IWL52_000185"
     CDS             complement(5234..5707)
                     /gene="tsaE"
                     /locus_tag="IWL52_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9776780.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5830..6810)
                     /gene="pta"
                     /locus_tag="IWL52_000186"
     CDS             complement(5830..6810)
                     /gene="pta"
                     /locus_tag="IWL52_000186"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9776781.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6963..7643)
                     /locus_tag="IWL52_000187"
     CDS             complement(6963..7643)
                     /locus_tag="IWL52_000187"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9776782.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7769..8638
                     /locus_tag="IWL52_000188"
     CDS             7769..8638
                     /locus_tag="IWL52_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9776783.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8664..9179
                     /locus_tag="IWL52_000189"
     CDS             8664..9179
                     /locus_tag="IWL52_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776784.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9226..9789)
                     /locus_tag="IWL52_000190"
     CDS             complement(9226..9789)
                     /locus_tag="IWL52_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9776785.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9844..10023)
                     /locus_tag="IWL52_000191"
     CDS             complement(9844..10023)
                     /locus_tag="IWL52_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776786.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10100..10609)
                     /locus_tag="IWL52_000192"
     CDS             complement(10100..10609)
                     /locus_tag="IWL52_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776787.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10705..11085)
                     /locus_tag="IWL52_000193"
     CDS             complement(10705..11085)
                     /locus_tag="IWL52_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9776788.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11415..12164
                     /locus_tag="IWL52_000194"
     CDS             11415..12164
                     /locus_tag="IWL52_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9776789.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12348..13625)
                     /locus_tag="IWL52_000195"
     CDS             complement(12348..13625)
                     /locus_tag="IWL52_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776790.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13704..14348)
                     /gene="pcp"
                     /locus_tag="IWL52_000196"
     CDS             complement(13704..14348)
                     /gene="pcp"
                     /locus_tag="IWL52_000196"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9776791.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14547..15491)
                     /locus_tag="IWL52_000197"
     CDS             complement(14547..15491)
                     /locus_tag="IWL52_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9776792.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15493..16179)
                     /locus_tag="IWL52_000198"
     CDS             complement(15493..16179)
                     /locus_tag="IWL52_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9776793.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16652..16972
                     /locus_tag="IWL52_000199"
     CDS             16652..16972
                     /locus_tag="IWL52_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9776794.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            17133..18038
                     /locus_tag="IWL52_000200"
     CDS             17133..18038
                     /locus_tag="IWL52_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9776795.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18035..18808
                     /locus_tag="IWL52_000201"
     CDS             18035..18808
                     /locus_tag="IWL52_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9776796.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(18985..20409)
                     /locus_tag="IWL52_000202"
     CDS             complement(18985..20409)
                     /locus_tag="IWL52_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9776797.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20620..21066
                     /gene="nrdI"
                     /locus_tag="IWL52_000203"
     CDS             20620..21066
                     /gene="nrdI"
                     /locus_tag="IWL52_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9776798.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(21136..23118)
                     /locus_tag="IWL52_000204"
     CDS             complement(21136..23118)
                     /locus_tag="IWL52_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9776799.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGARQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23132..24028)
                     /gene="murQ"
                     /locus_tag="IWL52_000205"
     CDS             complement(23132..24028)
                     /gene="murQ"
                     /locus_tag="IWL52_000205"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9776800.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(24031..25092)
                     /locus_tag="IWL52_000206"
     CDS             complement(24031..25092)
                     /locus_tag="IWL52_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9776801.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25304..25831)
                     /locus_tag="IWL52_000207"
     CDS             complement(25304..25831)
                     /locus_tag="IWL52_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9776802.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(25898..27400)
                     /gene="lsa"
                     /locus_tag="IWL52_000208"
     CDS             complement(25898..27400)
                     /gene="lsa"
                     /locus_tag="IWL52_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9776803.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYRLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(27658..28779)
                     /locus_tag="IWL52_000209"
     CDS             complement(27658..28779)
                     /locus_tag="IWL52_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9776804.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            28905..29846
                     /locus_tag="IWL52_000210"
     CDS             28905..29846
                     /locus_tag="IWL52_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9776805.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(29989..30885)
                     /locus_tag="IWL52_000211"
     CDS             complement(29989..30885)
                     /locus_tag="IWL52_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9776806.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            31167..32507
                     /locus_tag="IWL52_000212"
     CDS             31167..32507
                     /locus_tag="IWL52_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9776807.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            32702..33145
                     /locus_tag="IWL52_000213"
     CDS             32702..33145
                     /locus_tag="IWL52_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9776808.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(33407..34825)
                     /gene="nhaC"
                     /locus_tag="IWL52_000214"
     CDS             complement(33407..34825)
                     /gene="nhaC"
                     /locus_tag="IWL52_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9776809.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(35229..36452)
                     /locus_tag="IWL52_000215"
     CDS             complement(35229..36452)
                     /locus_tag="IWL52_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9776810.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(36484..37323)
                     /locus_tag="IWL52_000216"
     CDS             complement(36484..37323)
                     /locus_tag="IWL52_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9776811.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(37340..38020)
                     /locus_tag="IWL52_000217"
     CDS             complement(37340..38020)
                     /locus_tag="IWL52_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9776812.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(38022..39095)
                     /locus_tag="IWL52_000218"
     CDS             complement(38022..39095)
                     /locus_tag="IWL52_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9776813.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(39745..40416)
                     /locus_tag="IWL52_000219"
     CDS             complement(39745..40416)
                     /locus_tag="IWL52_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9776814.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(40504..43182)
                     /gene="mgtA"
                     /locus_tag="IWL52_000220"
     CDS             complement(40504..43182)
                     /gene="mgtA"
                     /locus_tag="IWL52_000220"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9776815.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            43842..44279
                     /gene="ndk"
                     /locus_tag="IWL52_000221"
     CDS             43842..44279
                     /gene="ndk"
                     /locus_tag="IWL52_000221"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9776816.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(44301..45167)
                     /locus_tag="IWL52_000222"
     CDS             complement(44301..45167)
                     /locus_tag="IWL52_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9776817.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(45401..47479)
                     /locus_tag="IWL52_000223"
     CDS             complement(45401..47479)
                     /locus_tag="IWL52_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9776818.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            47972..49189
                     /locus_tag="IWL52_000224"
     CDS             47972..49189
                     /locus_tag="IWL52_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9776819.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(49648..50271)
                     /locus_tag="IWL52_000225"
     CDS             complement(49648..50271)
                     /locus_tag="IWL52_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776820.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(50427..51824)
                     /locus_tag="IWL52_000226"
     CDS             complement(50427..51824)
                     /locus_tag="IWL52_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9776821.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(51846..52817)
                     /locus_tag="IWL52_000227"
     CDS             complement(51846..52817)
                     /locus_tag="IWL52_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9776822.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(52824..55124)
                     /locus_tag="IWL52_000228"
     CDS             complement(52824..55124)
                     /locus_tag="IWL52_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP9776823.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(55126..56451)
                     /locus_tag="IWL52_000229"
     CDS             complement(55126..56451)
                     /locus_tag="IWL52_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9776824.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            56682..57107
                     /locus_tag="IWL52_000230"
     CDS             56682..57107
                     /locus_tag="IWL52_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776825.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(57605..58306)
                     /locus_tag="IWL52_000231"
     CDS             complement(57605..58306)
                     /locus_tag="IWL52_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9776826.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(58322..58762)
                     /locus_tag="IWL52_000232"
     CDS             complement(58322..58762)
                     /locus_tag="IWL52_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP9776827.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(58762..59337)
                     /locus_tag="IWL52_000233"
     CDS             complement(58762..59337)
                     /locus_tag="IWL52_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9776828.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(59748..60254)
                     /locus_tag="IWL52_000234"
     CDS             complement(59748..60254)
                     /locus_tag="IWL52_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP9776829.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(60265..60744)
                     /locus_tag="IWL52_000235"
                     /pseudo
     CDS             complement(60265..60744)
                     /locus_tag="IWL52_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(60916..61617)
                     /locus_tag="IWL52_000236"
     CDS             complement(60916..61617)
                     /locus_tag="IWL52_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9776830.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(61725..63167)
                     /locus_tag="IWL52_000237"
     CDS             complement(61725..63167)
                     /locus_tag="IWL52_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9776831.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(63181..65046)
                     /locus_tag="IWL52_000238"
     CDS             complement(63181..65046)
                     /locus_tag="IWL52_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9776832.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIETIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(65167..>66000)
                     /locus_tag="IWL52_000239"
     CDS             complement(65167..>66000)
                     /locus_tag="IWL52_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9776833.1"
                     /translation="LNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAVQKEFVL
                     RDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLHYAITRA
                     KENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHFVNSQQG
                     NEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLKGEGSDE
                     QDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVSEKKSIQ
                     S"
BASE COUNT        19100 a        13963 c        11495 g        21442 t
ORIGIN      
        1 tcctgctctt ttacttattt taagttccat tacttattaa ttggctaaac ttctgcttgt
       61 ttctgatatt gcttgttttt ctccaatgat ccgcacttct gtttctaaag acacatcaaa
      121 tttttctttg attacttctt ggatgtgcgc aatcaattca atataatctg tcgctgtcgc
      181 atgatcgata ttgacgatga atccagcatg tttttcagag acttgtgcac cgccccattt
      241 caggccttgc aatcctgctt gttggatcag ctgacctgta tagtgtccta ctgggcgctt
      301 aaagacactt ccgcatgaag ggtattctaa tggctgtttt gattgacgca attcggtcaa
      361 ctcatccatt cttgctttga ttgcttcata atcccctgtt tgcaaactga atcgagcttc
      421 caatacgata gcacgtaatt cttggacttt actgtgacga taagaaaata ccatttcgtc
      481 ttttgtcaaa gttttcaatg ttccatcttc aagcaaaaga tccacttcag caaaaacatc
      541 tttgatctct ccgccatacg cacctgcatt catataaacc gctccaccga cacttcccgg
      601 gatgccgcaa gcaaattcaa atccagtcaa tgattctgcc aaagctgcat aagtcgtatc
      661 aatcagtgag gcacctgctt ccgcaatgac catcgttcct ttgacatgaa tctgtttcat
      721 ttcagtcagc atgatgacta caccgcgaat cccgccatct tgaacgatca gattgcttgc
      781 atttcctaat accatccaag gtatatcatg gattcggcaa taatcgatga tttgctttac
      841 ttcttcttgt tttttcggga atgcaaggac atcagctggt ccacctgttt tcgtaaacgt
      901 atagttcatc aatggttcat caaacaatat agtaatcgtg gctaagcttt caactatttc
      961 ttctttattc actgaggatt ttcctttctt ttctcaaacg tcctcattat tctatcacgc
     1021 ttaatacaat cttgccagtc ttgttcaaag ttgatgacgg tagtgatctt taagcacttc
     1081 ctcacataat tcatgacttc ttacaacttc tttttgttta agcaattctg tttccgattt
     1141 acctaaacgg atctgagaaa agaaggcctc taccattggc tcaaacccgc gtttgactag
     1201 cgtgtttgtc cagtcgccaa attctttcac tttttttcca tcagcatctt gaatgacaag
     1261 ctgggtcaga tcgtttactt cataagtacc atttttactt gttacttgat atttttctat
     1321 attagcgcca cttgctaaat ccatcgttaa aatagccgtt tgattttgtg tttctaattg
     1381 taaaatggca tattccaaaa attcctttgt ttctcttatc tggtattttg ttctcaaaat
     1441 gggttcatct aataaataca cagctgtatc aataagatgt aaaaacaaat cataaaggac
     1501 gaactctgtc gtttcttttg ccgctattcg atttttttgc aggtggatca accgcttatc
     1561 tgggatacgt ttcaattctt ctaccatagg agcaaagcgc cggttgaaac cgatcatcaa
     1621 aattatttgg ttcttatcag ctaattcttg caattcacga acttcattta gatggacact
     1681 cagtggtttg tcaatataaa catggatacc attttctaga cattttttgg ctaattcata
     1741 atgtactttt gttgctgcgt ggatcataca tgcttcgatt ttttcttcta aaagctcatc
     1801 gatcgtttca tatagatgtt ggaacccgta ctctttttga attctttctt ttgtttcttt
     1861 atttcttgtt gcaaaataaa aatcaagtgt tccttgtttt ttcgcataag ttggtaaata
     1921 tgctttttct gcgatattcc ctacaccgat tacaccaatc ttcatctact ttcctcctgg
     1981 atcatttagt tactttcatc ttacaccatt ttttactaaa atgaggtaca atagtatcgc
     2041 aaaggtaagg aaattaattc gaaggagtgt gcttttattg aaatttatct cttggaacgt
     2101 aaacgggctt cgtgccatcg taaataaaaa ttttttagaa gtttttcatg agttagacgc
     2161 cgattttttc tgtctgcagg aaacgaaact ccaagcagga caaattgatt tagatcttcc
     2221 aggttattat caatattgga attacgcgga acgaaaaggc tattccggca cagcgatttt
     2281 tgctaaaaaa ccagcattga atgccactta cggaatgggt atcgatatcc atgatacaga
     2341 aggccgtctg attactttgg aatattccga tttctttctt gtaacctgtt acacgccaaa
     2401 ttctcaaagc gaattaaaac gtttagatta ccgattagaa tgggaagaag ccttttataa
     2461 ttatttggaa aatctaaaaa aacaaaaacc agtcatcgtg tgtggggatc taaatgtcgc
     2521 tcatcaaaag attgatttga aaaactggaa aacgaatcaa aaaaatgctg gattcactcc
     2581 agaagaaaga gcggctcttt ctcgactgtt ggataacggg tttatcgata cattccgtta
     2641 tttttatcca acccaggaag gtgtctactc ttggtggaac taccgattca attcaaggaa
     2701 aaacaatgct ggttggcgga tcgattactt tttgacgagc aaagatttag agccaggact
     2761 agctgatgca aagatccata cggacattat gggcagtgat cattgtccag tagaattaga
     2821 tttgaaataa agaggttgtg aaaaagtcgt ttagtttcga gtattaagaa aaattttgtg
     2881 aaaatggctt tccacatttt gcataaattt ggcataatac cgagaaactg gcttttgaac
     2941 actgtttatc gagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
     3001 aaaaatagtt cctcatattt ttcgtttggt tgggcttatt accgcagata caagcttttg
     3061 aacaccgttt attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca
     3121 aacaaaaata gttcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt
     3181 ttgaacaccg tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag
     3241 ccaaacaaaa atagttcctc atatttttcg tttggttgga cttattaccg cagatgcaag
     3301 cttttgaaca ccgtttattg agaagttgtg aaatcagcgt cctgacctga gtattagaga
     3361 aacaaatggc aaaatagttc ctcatatttt gctatttttt gagctaatac cgagggtcag
     3421 ctgattgaac accgtttagt ggagaagttg tgaaaaagtc gcctactttc gagtcgtaag
     3481 aaattttttt gaaaatggtt cctcacaact ttgcataaat ttgacttaat accgagaagt
     3541 catgatcccc atctaactcc aaatcacaag gaatgagttc aaaaactagc ttttctcaac
     3601 aaatattaca taaaaaattc tatgtttttt gttaaattta tttgcttttt tcaaaagtat
     3661 gttacagtaa tttcccaagg aggttttaca gaaatgaatt ttgttgccat ggattttgaa
     3721 actgccaatc atcagcctta tagtgcttgt tctcttgcgc tagtcatggt aaaaaatagc
     3781 cagatcgttg atgaattcta tacattgata cagcctgaga ctcctttttt ctggcgaaat
     3841 gtccaaattc acggaatcca tcaagaagac gtacgcaatg cgccgaaatt cccagaagtc
     3901 tggcaaacta tccaaccata ttttcaaaaa aatcgtttag tcgttgcaca taatgctgct
     3961 tttgatacaa aggtcttagc gggatgctta gattattatc atcttgaaca gcctaattat
     4021 ctttctttat gtacagtgaa aagtagccgt cgcctgtttc ctgaatttcc aaatcatcga
     4081 ttgaacactg tgtgcgaaaa tttgaacatc caattagatc atcaccatga tgcattggaa
     4141 gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa aaaaattcgg gacagatcca
     4201 ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg aaacacacaa agaggtcgtg
     4261 agcctgccgt ttcactccga gtcacaagga gcaatttcaa aaaatagctt ctcatgttta
     4321 ttaaatattg tgactggtga ccgaggagct ggcaggagaa caccgtttat tggagaggtt
     4381 gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
     4441 ttttcatttg gttggactta ttaccgcaga tgcaagcttt tggacaccgt ttaccaagag
     4501 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca
     4561 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttattcaaa
     4621 aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc tgccttctca
     4681 aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat aactcatttt
     4741 atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga ccgcttcgat
     4801 ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt gaacgtcaag
     4861 ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt ctagcataag
     4921 agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt ctcccacgtg
     4981 agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg tcccttcata
     5041 gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact cttcttctaa
     5101 ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg tctccttatt
     5161 aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt cccgcaagat
     5221 gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc tgtttgaagg
     5281 tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa tctgtatcac
     5341 ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat agatttcccc
     5401 attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa tctgctccgc
     5461 tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga ctatattctc
     5521 taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct tgggcaattc
     5581 ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga ttgtcacctg
     5641 gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat cctgatagtt
     5701 cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg cgtaatgaag
     5761 tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg gctagttcgc
     5821 gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt tatatacatc
     5881 ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt gtagaattgg
     5941 tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat ttcctgactg
     6001 caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg gcgctttcag
     6061 ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga tcatgtattg
     6121 tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt ctggcgattt
     6181 cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat cgaacaattc
     6241 tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg gattcacgtt
     6301 gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca tgatcatcgc
     6361 tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg ggcgaacagt
     6421 gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca tatacgtaag
     6481 catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt tggatgcctt
     6541 tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt aattatcagg
     6601 gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat ttcttatttc
     6661 ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc gggcagctgc
     6721 gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac ggcgaacaat
     6781 tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt ttatatgtat
     6841 tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag tcaaaaatgc
     6901 tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa agcgctgttt
     6961 tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc aaggagcgca
     7021 tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga taacggactg
     7081 ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat tttttcttgt
     7141 gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa tttttcaatg
     7201 atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg cccttcccga
     7261 acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac tagatttcca
     7321 tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt tcttaacgat
     7381 ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg attttctcca
     7441 tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt caattccaat
     7501 gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc tttttttaaa
     7561 aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac ttcttgccaa
     7621 ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata actcacattt
     7681 tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt aaactaggaa
     7741 caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag atatggacgg
     7801 cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga tacgttttgc
     7861 aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag aagctcgcgc
     7921 agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg cacaggtatt
     7981 tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag caatctctgt
     8041 tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg atggtcttta
     8101 ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta cccatttacc
     8161 tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt tattgcacat
     8221 cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag ttttaaaaat
     8281 tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc agatttctct
     8341 tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga accataaaaa
     8401 cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt ctttagaaga
     8461 agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag caggagtcag
     8521 tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata caacggacac
     8581 aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag acttgtagtt
     8641 ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag cagcttggac
     8701 gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg atggttggtc
     8761 gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat ctcgttgctc
     8821 acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa gagttcgaaa
     8881 aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat attcgtagac
     8941 ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat catttcaatc
     9001 agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat gtattggatg
     9061 tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta gattactggc
     9121 tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac ttaagttaaa
     9181 aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga tattcttttg
     9241 catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga agtcatggcg
     9301 ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga tgacggagcg
     9361 attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga tgccttgtcc
     9421 tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa acgtttcttt
     9481 atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag gtaccagtgg
     9541 ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg caactttacc
     9601 gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat cagtgattgc
     9661 ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg gcagttccat
     9721 atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt ttctcgcaaa
     9781 acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga aatcgcaaaa
     9841 tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag taacgattct
     9901 tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat tatgttcgac
     9961 tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt tcattttttc
    10021 catgttatct caacttcctt tcatctttac tctcttagct tactcttata ttgtaaaaaa
    10081 gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt tctccatcta
    10141 ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac tctttcactt
    10201 ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt tgtttgaact
    10261 cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca tttcgtacgg
    10321 ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa aactggtatt
    10381 catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga atagcagata
    10441 tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat gagatccgat
    10501 attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc gtcataccgg
    10561 taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt cttcattctc
    10621 ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga atgaaacgaa
    10681 aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc cgtctggatc
    10741 aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt ctgattcgat
    10801 ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga aagcaagatg
    10861 acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa ttccaaaaat
    10921 ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt ctttgcggta
    10981 attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg atttctgata
    11041 atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag cactcctatt
    11101 cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg tcagattgaa
    11161 aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa agtaggtcct
    11221 ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat attttcaaga
    11281 aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta gttttttttc
    11341 aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt tagtgtctta
    11401 aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta aaaggctggc
    11461 cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg actcttgtca
    11521 atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt ggtgtattgt
    11581 gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt ttgtctgcga
    11641 tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt gaacagattg
    11701 cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat cataatatgg
    11761 cttcaaaaat ccgaaaatta actggaaaaa gctggtttat cgcaatcatc agtactttcc
    11821 ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct cggccgtggg
    11881 tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc ttgcgttaca
    11941 acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt tggtatattt
    12001 catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt tacttgctga
    12061 atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt gaacgtatgc
    12121 aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac tgagcgtggg
    12181 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaatcagaa
    12241 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
    12301 tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttttta tagtaattcc
    12361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
    12421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
    12481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
    12541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
    12601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
    12661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
    12721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
    12781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
    12841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
    12901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
    12961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    13021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    13081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    13141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    13201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    13261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    13321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    13381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    13441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    13501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    13561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    13621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    13681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    13741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    13801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    13861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    13921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    13981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    14041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    14101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    14161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    14221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    14281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    14341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    14401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    14461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    14521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    14581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    14641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    14701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    14761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    14821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    14881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    14941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    15001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    15061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    15121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    15181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    15241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    15301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    15361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    15421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    15481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    15541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    15601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    15661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    15721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    15781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    15841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    15901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    15961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    16021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    16081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    16141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    16201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    16261 agacactatt tttaaatatt tttcgttatt tttttattat ataattcacc ttacttattg
    16321 taagataaag ctttttttaa tttattgcct gctaatatat aaaaataagg tctagttact
    16381 gtttttttaa atgattaata aataacaact cctttctatg aaattgcaaa gcattcatct
    16441 tcttttctgc taacgatata tccgtcaatg cttgttacac tttactaaaa agtatgagat
    16501 tgccgatatc tggataggtg tgctatacta cagaaaaaga tatacgatct gccaccgggt
    16561 agtaaaaaag aggatgatca ttccattagg tctttgaagg gatgattctc ctctttttgt
    16621 gtatttttta tattgaagga ggaacgtatg tatggcatgg atggaattaa tcatagcagg
    16681 aattttggaa gtattctggt caacgatgat gaaatggagt gatggttttt ctaaaatcaa
    16741 ttattctttt tacacagtga ttggaatgat tgctagtttt tactttttgt caaaggcaat
    16801 caaaagtttg cctatgagtc tctcttatcc tatttggaca gggattggcg cagttggttc
    16861 ggtcattatt ggtgtggtct tgtttcatga taagctgaat ctttccactt ggttctttgt
    16921 cgggttgctg ctcatcagca tcattggcat caaaatcact agcggtcact agatacttag
    16981 acaagcagac aaggactgat cagaaaaagc ggggcttttt atttgatatt cctctttcta
    17041 tgaaaaaaca agaaaataag actaatgacc tagcgacttc tctactcatc aggtgtatgc
    17101 ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc caagaattat
    17161 ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta tctcctggca
    17221 aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg aaagctattt
    17281 taggactgat ccattactca gaaggaacga tccgtattgg tgaaaatgag atttctccct
    17341 ctagtcataa aggattagaa caagtaggag cattgatcga atatccgggt atctatccat
    17401 tcttaacagg ttatgatcat ctgaagttat tctctgaaac aaacgatgta tctgctatcg
    17461 acactatcgt gaaacagctc aagatggaaa aatacattca caaaaaagcg aaatcctact
    17521 cgctgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat ccgtctatcg
    17581 ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat gtgcgaaaaa
    17641 tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac ttactcagcg
    17701 agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaaaatc atcaaacaat
    17761 gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct acgacaaatg
    17821 atcaagctgc tcgaacgatc ttgacagacg ctggatatcc cttggaagat cctgataaaa
    17881 tcaagcttct aaaaaaaaac gaagagttat taggaactgt tatccagtta ttgtctgcag
    17941 aaaatgtcca tgtgacagat gtccagcatc tgacgaatga tttggaatct tccctattgg
    18001 aaattctgga ttcagtaaag ggggaagaag aaaaatgacg ttgcttataa aacaagaatt
    18061 atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag ttgctttgct
    18121 cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag ttgaaatgac
    18181 tgctcagctt ttcagtgcca catcttggat tgtctttatc atgattgctg ctgcaagtac
    18241 gatcatttca atggaatcac aatatggtac attgaaaaat ctcctttatc gaaagtattc
    18301 tcgaggagaa atattagtaa gtaaatggat cacattagtt atttattcta tttatttata
    18361 ccttttagct attattgtca ccgtgctgat gaaactgatc cttttccctt cgatttcctt
    18421 tacggagaag atttctactg gtcagacact gatccaaagc ttattcactt atactttggg
    18481 tagttatatc ggtttatggc taattttaag tttagtgttg atgattgctt gttttatcaa
    18541 tagttccggt gcctccatct cagcagggat cgtcttttat tttgcttcat caatcatatc
    18601 tggtatcctc atcgcactca ttcaaaaatg ggagtggatc aaatggaatc caatcagtat
    18661 gctgaatctg caaaaccaaa ttggcaacga agagattatg aagcaattga ctcatctttc
    18721 tactaatcaa atgctgtttg gcaatttagc ttacattgct ttgttcttag cattgggata
    18781 cctcgtattc aagcgaaaaa atgtatgatg gttcattaac aaagaagttg tgacagaagt
    18841 ggccagtttc aagaaatttc ggcttatttt tagaagaagc tacttcttga acaccattta
    18901 ttcaaaaaaa gaagcgatga cacgaatgtt gtcaccgctt cttttttgaa taaaccacag
    18961 gcttcacagc ctctctttat acttctatac gtgaagcatc ccagtggcaa ataaaaccat
    19021 tgcgattact ccgaccatcg tcacaccgat aagtaaaatc ttatctaagt ggatatgttg
    19081 tacaggttcg ttttgttctt tacgtgcttt gaggtaaaaa taaatacctg gtaagaaaag
    19141 aacaagaaca agcatcactt cattaaaggc tacaactagc atggcccata cttgaaaagc
    19201 acttgctaaa aaccctacta aaaattggac ccactctttc cgctgataag aaagcttcat
    19261 ttggtacagc cctaagaaaa gccacgtgaa gaaaatcgct gctgtaccaa gtgtataagc
    19321 aaagttatat gcactgtcag tgaataataa tgaaagtaaa aagacattcg tacataacgt
    19381 cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc atgctttagg
    19441 caaaagtccg tcctcagcca tattccccat tgtttcaatc ggcaagattg tccatgagag
    19501 ccaaacccca actaaagaaa tgatcaaacc gccgttgatc acgaccgccc cccatttacc
    19561 aaccatcatt tcaaatacat atcccatagc aggttgcgct ttgatcgttt gcagctgatt
    19621 catcgtcatt gtgccatatg gaagcatgga aaccaagacg taaataatga tcaaaccgaa
    19681 tattcctaga atcgttgctt tcccaacatc cgattgtttt tttgctcgtg aacctacgac
    19741 agaagctcct tcaataccaa cgaataccca gagcatcacc atgatgcaac tcaatacttg
    19801 actagaaaac gggatcggct gcccattcgc attcaatatg atatcgctag aaaagttatt
    19861 aatgaattgt tggatcgaaa aggtgctgat agctactaca ataaataaaa ataatggcac
    19921 taatttacaa aatgtgacga tagcattgac aaagctagca ttttcaactc cacgattcac
    19981 tagccatgca tatccccata agaagatcgt actcatcacg atcgatgcca cattttgacc
    20041 accttcgaat acggggaaga aataacctaa tgcactcatc aacaatgttg cgaacgccac
    20101 gtttcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc
    20161 aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt ttttattgat
    20221 attattgatc gataaggaga gcatcaacat tgctgttcct acgagtaccc agctgaagat
    20281 agctgggcca ccagccgatc cggagatgtc cgtcataata ccaaatacgc cacctccaat
    20341 cgctgaacca atcactaagg cagcaagtgg aatcaaacca attccttttt tctgttgctc
    20401 tgtatccatg taagcatcct ccaaagtaaa accgctttta tcaaaaagta taacaaacct
    20461 tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac cgactttttt
    20521 ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa aaaacttaaa
    20581 aagatgctat actaaaggga aagaattggg ggatgatcca tgaacatttt atatatatcg
    20641 atttcaggaa atacgcgtgc ttttgccaaa catctagcag aatatgctga aaaaatgcat
    20701 gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt
    20761 acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg cgggaatggg
    20821 ttagacaacg gtgacacaga aatcttgacc gaaacgatgc gcgaatatct ggaatacgaa
    20881 gacaatcata aactttgcct tggtgtagtc ggtagcggta ataaaaactt caacaatcaa
    20941 tattgtctga cagccaaaca atacgcacaa agattcggct ttcctttctt ggcagattat
    21001 gaattacggg gaacaccatc tgatgtgaca cgcgtttatc aaattttggc agaaaaaaat
    21061 aaataacaca aaagaagaag ctgaaaaata aatttcggct tcttcttttt atgttctcta
    21121 ttgtttctat tcttattaag ctttgacatc catcacaggt gcttttgctt tcgctttacc
    21181 tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga cgatcatgga
    21241 ggtttctttg tctgctgctt tgattgcttc taagtcagct tctgctacaa gcgtatcagg
    21301 tgtcacttgc tgtccttctg ttactttcac atcgaatggc gtaccgccta agtcaactgt
    21361 gttgatgccc atgtgaagca agatttctaa gccatttggt gtcgtaatgc cgatagcatg
    21421 ttttgttggg aaaacagaac ttattactcc agtaaccggg gaatagatat gtccgttttc
    21481 tggttctaca aagtaacctt cacccatcat tttttgtgca aaaactgggt cacttgcttt
    21541 atctaaagaa acaactgttc cgtcagcaac tgatgataag atagtcacac tttcagctga
    21601 agcttgaggt actgcttttt caggtacttc atttactgtg gctgtttcgg tcgctagttc
    21661 ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga agccaccagc
    21721 gtaagcagcc aataaaccaa ggatatagcc taaccattta ccatcagaga tcaatggaat
    21781 caaagcggct cctgaaggtc caatagcgat tgctccgaca tttccgatca ttccgatcac
    21841 tgccccaccg atacctccac cgatacaagc agtaataaat ggacgtccta gaggtaatgt
    21901 tactccgtaa atcaatggtt cgccgattcc taagattccg actggtaaag cccctttgat
    21961 catttctgtt aattctttgt cttttttgca gcggatccat aatgccaatg ctgcacccac
    22021 ttgtcttgct ccggccatcg caaggattgg aagcaataag gtacgaccag tttcatcaat
    22081 catttgtaca tggattggcg tcaagatctg atgtagtccg aacatgacca ttggcaagaa
    22141 taatgtacct agaacgaagc cggcaaatgc tcctccaaca ccaagtaccc agttgatccc
    22201 accgaccatg ccgtcagaaa tgaaccctgc taaaggcatg atgaagaaaa tttcgatcag
    22261 accgatggca atcagggaaa gagttggcgt aacgatgata tctaccgcat caggtactac
    22321 tttatgcagt tttttctcaa cgattgacaa taaccaaact gcgaagataa cacctaagat
    22381 cccgccttgt ccagttgcaa gagcttcacc ggtaaacaag tttttcagtg gtgcttctgg
    22441 attcatccct gtcagcatca caacagcccc gataactcca ccaagcgttg gcgttgcacc
    22501 aaaaacttgt gcagcattga tacctgtata gataactaga tagctaaaca tcccgttttt
    22561 caagatgttc atcacatcga tatattgttg ccatgttcct gcgtctagtg ttccagctgt
    22621 gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtgccga caaatgcagg
    22681 gatcattggt acaaagatat tagagatgtc ttttaatacc gatttgattt tagagggttt
    22741 ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc
    22801 agatgcagaa gaacttgctg tagttgcact agcagaagga agtttttctc ctaatttcac
    22861 gcctgctaag tcaaccattt cctgcgctac tttgttcact ttcccaggtc cgatgatgac
    22921 ttggagctgt tcatcctcta ctacgccaag tactcctgga atcgctttta attccgagac
    22981 atttacaaga tcatcattga tgaccttcat acgtacacga gtcatgcagt ggacaatgct
    23041 ttcaacattg cccataccgc ctgctccttg ataaatttgt tttgccagtt cttccaaagt
    23101 caattcgttt tttgccatgg tcttttcccc ctcataaggt tccttttaca aaaccgtttg
    23161 cttcagctaa ttttgcttga gctgcctctt tatcactatt tgtcaaaatc atgacgatag
    23221 ccagtttgac attttgatct gcttcctcaa agtacttttc agctgtttcg tagtcagctt
    23281 ctgtcgcttg catgatgatt cgttttgagc gttcaattag tttttcattt gttggtttga
    23341 cgtcgaccat caagttattg aatactttac cgataccgat catactgata gtagaaatca
    23401 tattcaaaat cagtttttgt gctgttcctg acttcaatcg agtagagcct gtcaaaaatt
    23461 ctggtcctgc atcgacttcg attgggaaat ccgcatgctc gctgatttct gcttgtttat
    23521 tgcaagaaat cgttcctgta acagcaccaa tcgattttgc gtaatctaat ccaccaatca
    23581 catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt gttaaattca
    23641 gcttgattag atcttcttgc cctaattcat aagaatcttc ggctccttcg acggctacag
    23701 tcattgcttc ttctccacca gcaattaggc ctatgaccat ttccggttct acaccgaatg
    23761 tcggtacaca ttcagcagca tctaatacac caagtctacc actcgttcca gctcccatat
    23821 aaatcaagcg accacctttt ttaaaggatt caatcgtgtt ggcgatcact ggctcgatac
    23881 ttggcagttc tttttcaaca gcagcggcca ctttctgatc ttctttgttc atgagttgga
    23941 ctgcttgaga aatactcatt tcatctaatc caaaagtttc ttggtttctg cgttccgttg
    24001 ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta taaattgata
    24061 gttcatacca gctttgattt ggttgatcag cggtagttct tctttgatga ctttggctac
    24121 tacattcact ttttcatccg ctggcaaatc atatttagtt aattggatct cacccatata
    24181 gcgcaaatac ttttcgttat ctaaagtaat agatcctttc atacgcttag ctgtatttct
    24241 tactgggatt ggcggaatct ctcgaaaacg cgcatctgcg cttcgtatga catctcttgc
    24301 atcatcttgc cgattcgtat gttttcctaa tatatattcg taaaaaactt catctacagg
    24361 ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct cctgaacgtg
    24421 ttctgacaga cctgcatctc cgatataaac aatatccgtg ttacttgcta atagatcaag
    24481 agcacctgcc agaggatgga tccctcgatg ttcttccaaa gttggtaatc cttgatacag
    24541 tggacctctc aactctgtat ctcctggtac aaagccttgt acaaggaatc cttgagtttt
    24601 gagccattga tttttttcct gataccattc tttgtccaac cctgtttcaa gacgtggata
    24661 ataattatgc caagcttcaa tctgtgagaa atcagccgct gcttctttca actcatcgat
    24721 atcattaggc gtgatcgtgc tggcattcaa actgattttc aattgatgtg accagtcggc
    24781 gatctgctga ttagaaatat gataatccat tcttaaccct gttacaccaa ttgctttcaa
    24841 gggtgttaga tcttctaatg aaaagccagc tcgtttcaat gcctctcctg aaatatcgac
    24901 catcaactct agtttattct ttttcgcttc gcttcctagt tcgatcaatc gattttgata
    24961 agccgaaaca tcatcttccg gtatatgcat ggaagtgaaa ataccgacaa agccattgga
    25021 tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcattca taaatattga
    25081 aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg actagagtat
    25141 atatctaaag aaacaaaatt tcaatacttt tgttaaaata attaaatttt atttctaaaa
    25201 attggattta taatgccaaa agaattgaca cagccacaaa aaaggcactg ctaagcaaaa
    25261 gcagtgcctt gaaacatttt atttaaataa cacaaaagtc taattactca taagaaatta
    25321 attctgtatt gtctaccatg aatgtaaaga acccttcatt aggcattgga tgaaaataag
    25381 cattgatcac tcttatcaag gcattatgac agactaacaa gtagacattt tcttcgtctt
    25441 ccatgcattc tttcaataaa ggaacgatcc gtgcgtacac atctaagacc gactctccat
    25501 ttgggaaacg tacagcaaac gctaaacgcg ctttttgaaa gttttcatct ttgcttggca
    25561 gtccgtcata atccccaaaa tccatttcta ccaatcgctc atcagcggtc atcggcaaat
    25621 tgagacgatc aactactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    25681 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtgcctgt gcctgcatct
    25741 gtcctttttc agtcagcggc acatctgctc gcccgcaaac taatccttgt gcgttccaag
    25801 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg acaaagttct
    25861 ccttaaccat accttgttgt ctttgagatt acaaggttta gattttctca agagaaataa
    25921 tctcagtaga tactttatct agaaaggttt gatcatgttc cactagtaac atagcaggtt
    25981 tcacgttcaa aatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    26041 gttcatccca tatatagagc tcagctggtt gggataaaga tttagccaat tccacttttt
    26101 tccgttgtcc catactcatc tgttcgattt tattatgaaa aacatctctt tccatcccaa
    26161 gctttcggag attattcaaa aatgcttgat agtctactcg gtttttcttc gcgaattccg
    26221 ccaatgtccc tcgattgtct tcataatttt ggcgtacgta actaatggtc aaatgtttgg
    26281 gctgggattt ttctcctaca accttgccgt tgaatgtccc cagacgataa tggataatgg
    26341 atgactttcc cgcgccgtta ggacctgaaa ttgccatccg ctgatgaggt tcgatcgtaa
    26401 aatgaactgg ctcgaataac agattttcat accctaattg aagattttct acgcttaaca
    26461 gccgtttatg atgagacact tggctattca tcgtcaaagc atcgatatac tcgatatctt
    26521 ttaataattt ttctttctca ctgatctggg tttccatccg attcacgatt gctttagatc
    26581 gtttcatcgt ccgtgcagca tcagcgccta ctgctccttt attcactcgc ctagattccg
    26641 tatcgatgaa tccgactttt ttctttgttt tgtccccttc tcgggaacga gaccattctg
    26701 ctttttcagc tgctgttttc tttagcctgc tgacttcttt tttcaatttt tcatttttag
    26761 ctatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    26821 aaagttccag ttggctcttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    26881 cccgatcatg actcaccacg atgaatcctt gttttttctt tttcaagtaa gccgctactt
    26941 gttttctgcc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    27001 catccacaaa taagagtgcc agtaggactt ttgtcttctc tcctcctgat agtgtgctga
    27061 attctctcca taagatttca ggatctgttt gcatcagttg gagctctctt tcaatttccc
    27121 atatctcaaa atccataatc tcatttaaca cataataggt caaacgctct ttatcttttg
    27181 tttgctgcgg gaaatagaca aacgcttgct gatggatcac ttgcccttga taagggagtt
    27241 tgttttgcag gatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    27301 gtttccattg tgtgtcaaag tttagattgg cttgttcaaa taataatgtg ccttggctgt
    27361 catagccgaa tgtcagattt tttatttcga ttttagacat tattccatca cctcgtaaaa
    27421 aaagaccagt cctattttca gaactggcta aagaggatgc aagggttggg gcacacaaaa
    27481 agacccatac atcgaaagaa ccaagccaat cccgaaaatc tgcacactgc tcccttgtct
    27541 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    27601 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaattgttt ttgtcaacta
    27661 cttcgggaga atgttgaaaa atctgctgag tgtctgtcct aagatttctg ttttttctaa
    27721 tgtttgcctg tgttccattg cttcctctaa atcgattggt cctaattgaa tgcttaagac
    27781 aatggggaga aaatcaaaga gtccttccag ctctcggtct ctacttccgc aaatagcgat
    27841 tattggtttt cgatattttt ttgcaagcac tgctacgcca tatggaactt ttccgtggat
    27901 tgtttgctga tcaatccgcc cttctcctgt aatgatgaca tccgcttgtt cgatctgttt
    27961 ttctaatcca gtgatttggc tgaccaactc aaaaccagag accatcttcg ctcctaacaa
    28021 aaaaagggct ccgccaatac cgcctgctgc tccgcttcct gggatgtcat ctaaatccac
    28081 ctcccaaaca tcttttatct gctgagcaat cttttcagct tgtttatcca agaaagctaa
    28141 cgtcgtatcc gttcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    28201 atatgggttg gtcacgtctg cagcgatgat cacttttact ttagaaacaa gaacgttcct
    28261 tcgatccaaa tgaagttttc tagtagataa caaaggattt ccttctgtgt acccttctaa
    28321 agaagcaccc aaagattgga gtaatcctag accaccatcg ctacatccgc ttcctcctaa
    28381 agtgacaatg atttgttcca cttcttgaag cagtgtatct ttgataagct ctcccaaggc
    28441 ataactagaa gcaagtcgga tggtttgatc cgttggttta tcaagtaaat ctaatcctaa
    28501 tacttgtgca gattcaatga ctgcggtatt ttttccttgc cagttcgtca ataaataact
    28561 gatttttcgc tgacgaaata agagatccat cccatcaatc gtacgccagg ttcccccgag
    28621 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    28681 tcttccttta cggtcttccc aaccatttag aactgcctga tttaattcaa atgaagaagc
    28741 acttccttta aatgaatcga ttgccgccaa aatatccatc tatatccctc actttcttct
    28801 taagtaagac actttttttc acttttttca aacaaaacaa tgctattttc ttctgttttt
    28861 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    28921 ctggagccgg agcaatgggc agccgaatcg gattaatgtt gcatcaaagc ggaaatgagg
    28981 tcgtcttgat cgatcgctgg cctgctcaca ttgaagccat tcgtacaaat ggattgattg
    29041 ctgactttaa tggcaaagaa gtagttgcaa aactgccaat ctattcacca gaagaaatca
    29101 tcgaaagcaa caaacacgtg gatttgatcg tagccttgac aaaagcgaat caattagatg
    29161 atatgtttcg ttcgatccaa tccattatta ctgagaatac ttatgttttg tgtttactga
    29221 atggattagg tcatgaagat gtactggaaa aatatgtacc aaagaaaaat atcttatttg
    29281 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacatta ttaggtgatg
    29341 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    29401 aagtctttca agaagcaaaa ttgaatccaa tctatagtca taatgttcgt tattccattt
    29461 ggcgaaaagc ttgtgtaaac ggaacattaa atggattatg cacgatattg gattgtaata
    29521 ttgctgaatt aggtgcgcaa aaagcggcag agggcatggt tcgaacgatt gtatctgagt
    29581 ttgcttctat cgcagcaaaa gaaggaatca cattagatca agaagaagtg taccaacata
    29641 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    29701 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccgtt tggcgaaaag
    29761 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    29821 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct ggatcagcag
    29881 ctggttctca tttttttacc cccatgaaag ttcaaaaaga aaaacacaat tctcatgttg
    29941 ccataagaat tgcgcttgtt tattttcatc tatgattggt tggcactttt atttattttt
    30001 aacagaggaa agatcgtatt catattgttc ataaatagcg gagtcagtag tgaaaaaacg
    30061 ttttgcccaa ttttctgcat caaaaaattc caacagttct gatgggattg gttgaaatgg
    30121 cgtatgcaaa aattccggta attcatctaa atcacgttct tgaagcaaaa aaatattttc
    30181 agaatgataa aactgataat gagctatggc ttgattatct gtcactagtt tcttttgaaa
    30241 atacatagat tcaaacgttc gcaaagtcac tccttgctgc ccttcccgca acagttctag
    30301 aatccccctc gattccccgg tttttttcag atattcttca taagtaaggt atccaggttg
    30361 ttcatttcct cgaagcggaa gaataaagta attggtcgta acacctcttt ttgtaaattc
    30421 ctgcttatat ttttctgccc gttcttttct gccgttattt gttgcaccaa aaaatagatc
    30481 cgtctttggt tcgctagaag aaaaatccat atgtttcgaa taaaaggaac tattatgttt
    30541 tagtccaagt tctttcgctt ctaaaaaatc aaaatgataa atctcatcga ttgccggttc
    30601 atcttgtgcc tgcttcagca tcgcctcgtg ttctgcttta aagtggttcc aaaaaaataa
    30661 cacgattttt gcattggtaa atggacgcac gcgtcgagcc atctccatat caaatccata
    30721 atctaaaaaa acgatcagct gataatttgc caactgttgt cgagctgatt taatgaattc
    30781 cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag gaaaaaaata
    30841 aagtgattta tttttcgcaa ttagcaatac gtcgtttttg ttcatctgtt ccgcctcact
    30901 ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag taaaacgctt
    30961 cctattattt ttataaaaat ttaacatttc tatgaaaata cgatgaaata aatcattctt
    31021 ccattaaaat atgcccaaaa gttttctgaa tagttgtaat tatctgaaaa tttgttatca
    31081 ttagtcatta ttattattac tatttattct tactatttat tcttactatg cattttgatt
    31141 gtgaatttaa aagaaagagg ttatttatgg aagataaaaa attgaaaaaa caacttagct
    31201 cccgacatat caccatgcta gctttaggtg gagcgattgg tgcaggtcta tttaaaggaa
    31261 gtggcgaagc gattggaatc gccggccctt ctgtattgat cgcttttttg attggcggca
    31321 taattttata tatcgtgatg aaaggtctgg gaaaaattgt tttaagtggt ggtgatacgc
    31381 accacggatt atctggattg attcgtccat atctaggtgc tcatgctgcc gattttaccg
    31441 attgggtcta ctggtccatg tggattatca atatcattgc tgaagctgtt gcagctgctt
    31501 cctttttaca gttgtggttc ccccacatcc cagcttgggt atttgttttt atgttggctg
    31561 ttttaactac tatcatcaat ctttattcgg tacgattatt cgcagagaca gaatattggc
    31621 tggcctttgc taaaatcagt gtcattatcc tattgatcat ttttgctgct tatcttgtcg
    31681 gtcagcaaat gttgggtact ggtgttttcc ctacccttca acaattaact gatcatggtg
    31741 gttttactcc ccacggtatg aagggaatca tcagttcttt gcttgtagtt atctattctt
    31801 acggcggttc tgagttgatt gctatcacag taagcgaagc agatgatcct aaacgtgcga
    31861 ttcctaaagc aatcaaaggc gtcatgggaa gaatcatttc cttttacatt attcctttat
    31921 ttttgctttt gattattttt ccttggaata ctttagcagg cacgaacgtt agtccttttg
    31981 tgatggtatt tgaaaaaatg aatattccat ttgcggcaga tatcgtcaat ttcgtgattg
    32041 ttcttgctct gttctcgtct atcaattcag gtgtatatgc ttcttcgaga attttgttct
    32101 tccgcttgaa agatcggaaa ggttcctcta gaaaactagc tgttttaaac aagcatcaag
    32161 taccgcaacg tgctgtgctg ttttgtacca gtgttcttta tctaggtgtg gctctttctt
    32221 attttgtcgg tgacaaatta tttggctatc tggcaggttc tctttcctat acagtattat
    32281 tgatctggat cattatcagt gctgcgtcct ttgtactgac actaaggaaa ggaacgttat
    32341 gggacaaagg aatcagcttt tttgcattag cagtcttatg gttgattttc cttggtatcc
    32401 tattcacgaa ctccattggg gtaactgtat tgaccggttt gctttacctg ttcatttatt
    32461 tcagttatca aaaaaagaat gatgctttcg tcttgacgaa tgaatgaaac caatagagaa
    32521 ttttattcta tttatattga atatctggag ctattgaaaa aaaggagctg cgacaaaaat
    32581 tgttgcagtt cttttttccg aataaacggt gttcaaaaac catctgtgca gattaattga
    32641 ttgttctcca cgctcatgac atgatagaag aaaggaatga agaaaaagga gtttgtttta
    32701 aatgaaaaag tattggaagt ttttgttgat gattggtgtt tcgacggtag tcatgtttgg
    32761 tatgatgtat ttgaatgttt atgcgcttga tcatatcttc ttcagtcgca cacgtgtatt
    32821 catggcgttg atgatgggtg cagtcatggc agtcatcatg ttgttgttca tgtggaaaat
    32881 gtatgaaaac aaaaagataa atgctatgat tttagccgct agtcttcttg tttttgccgg
    32941 ttccttattt atggtacgaa gccaaacaac tgtcaacgat acggcgtgga tgaaggcgat
    33001 gatccctcat cactctattg ctatcttgac aagtaaacga gctgatttaa aagatcctga
    33061 agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa gaagaaatcg acaaaatgaa
    33121 aaaaatgatc aaagaaatgg aatgaccatt atccatccaa ataaaaaaag aggatgttcc
    33181 agaagtgatc aacttcaaga aataagaaga agctacttct ggaacaccgt gaatcagagg
    33241 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    33301 atttttcgtt tggttaggct tattaccgca aatgcaagct tttgaacacc gtttattcgt
    33361 aaaagggagt gtgacaaaag tcttgtcaca ctcccttttt acgtttttat tgttctaatt
    33421 gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ctttgagatc ccccaacctg
    33481 taaaggcact gaccatcgaa aacacaggtg aaagcaaact gaaaaacacg aatggcaagt
    33541 aagtgagtgt aggaatgcca aatgtacttg ctacgaaact tccggcgatt ccccaaggca
    33601 ctaagtagtt gattactgtt ccaccatctt ctagtgttct ccctaataca gttggatcaa
    33661 atcctgcttt ttcgtaagtt tctttaaatg cattaccagg caggataaca gatagaaatt
    33721 gttctccaat aaagatattt actccgatgc ttgtcatcaa tgttgtaaaa atcaattttg
    33781 gggtgttcgt catctttctt gatactcgtt ccataacggt tgagatcaac ccaaattcga
    33841 tcaacaaacc acctaatgac aatgttagaa taatcaatga cacagttggc atcatgcttt
    33901 cgattcctcc acggctaagt agtgaatcga tttgttggtt tccagtattt gaaacaaatc
    33961 ccgattcgat gacccctgcc aacttcgtta aggaagtttg tgggttttga ataaagatca
    34021 ttcctactgc tacagcaata ttcaacaaaa tcgtcaaaat tgccggtacc tttttccatg
    34081 cacacaagag cattaaaaat agaggaatca gtgaccagat cgaaatagta aagtttgctt
    34141 ctaaaatatc agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta
    34201 aaatcatgaa taaaaagaaa gaaacaacaa atgccggaac agttgaccac atcatatgct
    34261 tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc taaatttgtt gattcggata
    34321 atggagacat cttgtctcca aaaacggcac ctgaaacaat ggctccaaca acaagggctg
    34381 gatttagtcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag
    34441 taaaggcact ccctacagcg ctacctacga ttgcacaaac aacaaataca gaagggacga
    34501 accactggac actaatcaat ttaaatccaa tgaccatgag ggttggaata attcctgctt
    34561 gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattgga atgatccccg
    34621 gtttgatgcc attgatgatc ccttcgtgca gccaatctgt cgggtatttt tttatcactg
    34681 cgtacaacat gaccattccg atagctgcca aaatcgtgat attaggtgaa acctttaatc
    34741 cgacaacgcc aatcgcaatg cctaaaacaa tgaccatcaa tacaagtgtt gcttctaaaa
    34801 aacttggtgt tccttttttt tccatgataa tctctccttt tttagttgtt cttatgttct
    34861 aaaaaagtta agatatcgga ttactccgcc acaattcatc cgcgattcct cctttataaa
    34921 aataaaaaga cctgctttca actaaggacg aatgactccg cggtaccacc ttaattaaaa
    34981 acagatctgt ttttcacttt caaacgatta ttcaaatgta tggactttcc atgtaattcg
    35041 tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccac
    35101 tactgcgtga aaagttgact tgtatgaagt ttaccttcca taagatcatt tgtcaatgaa
    35161 aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcgcg cacaacttta
    35221 tctttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc ttgatgttct
    35281 tgtttgattt tttctaatag cttgggttct ctcaataaat ctagagctgt attggcaagt
    35341 aattccgctg caatcgcaat agaagcaagt cctttttcac ttttagcagc agctttgaat
    35401 tcttctgtat gtccggcaat atatgtctca gagattgcta cagtaggctg tatcgttgga
    35461 ataacttggc taacatttcc tacatcagaa gaacctagac ttgttctttg ttccgtttct
    35521 atttcttcat caggaacccc tgcttctttt gcatgttgga agaataagtc atcgaaagaa
    35581 ggcgttacga tgacatcatc gactgcattt tggaacaatc caaactcaaa agtcgtaccc
    35641 gtgcttaatg cagcacctcg tacgatattt tccacttttt catatacttt gtctaatgtt
    35701 tgtcgatttc ctgcacgtaa atagaaacgt ccagcagcgt agtctggtat gacattggcc
    35761 gccacgcctc catctgtaat gattccatgg atattgacat cttttggcaa ttgcaagcgt
    35821 agagcgttga ttccgttaaa cacttggatc aaggcttcca aagcatttac tcctttttca
    35881 ggtgctgctg ctgcatgaga agcgacaccg taaaatttga tatcgactgg atcatttgct
    35941 aaagattctg tcgttttccc ataacgaaaa gctggatgga cacataatgc tgcatcgaca
    36001 tcctcaaaaa aaccttcccg aacgaagctt cccttagctg atccattttc gcctccttct
    36061 tcccccggag taccatacac acgaatttct ccgccaatct catcaatgaa ttgtttcact
    36121 atgctggctg ctaatacgga gtaagtacca aataaattat gtccgcatgc atgaccaata
    36181 cctggtaaag catcaaattc tgccaaaaag gcaattgtcg gacctggttt attggaaata
    36241 taacgtgcat caaagcctgt tcggtgccca gctacttctt tttctaccca gaacccttct
    36301 ttttccaatt gtttgattaa agctgctgag gaataaaatt cataattact tacttcagga
    36361 tggtcgtgga tagctagtgc aagtgcttga tattctggta aacggtattg aatcagatcc
    36421 ttcaattgat ttgttgagag tgtggttgtc ataaaaattt cctcctatgc tagtcgtttt
    36481 ttcttatttt ctcccgaatg tttcccacgc aggtacgctt gaaccttttg atgttttttc
    36541 gattactttg gctgtttctt ctgtttgata ggcatctact attttttgat acgttttatt
    36601 gtcttcatct tcttttcttg caacgatgat attgacataa ggacgtgcat gctcatcgac
    36661 tggttctaag aagattgcat cagaagcggg attaaatcca gcatctacag ctacaccgct
    36721 attgatgact gaaatatcta catctgacaa agcacgtgct gtttgggaag catctaactc
    36781 agaaatgtct agattttttt tattctcggt aatatcagaa acggtaggcg cttggccttt
    36841 tgccttgtcc actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt
    36901 cgtgacatca ttagggattg caacttttgc tccatctttg atatcagcca catcttttaa
    36961 cgtattggaa taaattccca acggtgcatt aaccgtattc ccaatcgata ctaaatcagt
    37021 tccgtgttct tcattgtaat tgtctaaaaa gaattgatgt tggaaggaat tcaaatcgat
    37081 cgatccatct gctaaagccg catttggctg attgtaatct gtaaatttca catattccaa
    37141 atggatgttt tcctttttca aacggtcttt cacatcgtcc catacatctg tatcttcccc
    37201 aatgatccct agttttacgg tcgtttcttt tttttcagtt gtagctgctt ttccttggct
    37261 gcatcctgcc aaccaaataa ttgctacaaa tgtaatgact gcaaaacgaa taattttttt
    37321 cattttttct cctccatttt caatgtgtga cttttttaat cagttgatta ctgataaatt
    37381 gactcaaaaa aactaagacc agaatcaata atgtagcgac aatcgttaca tctgtttgga
    37441 atcgattata tcctctcgat atagcaagat tccctaatcc tccggcacct actgctccag
    37501 ccatggtagt cagtccgatc acattgatga tcgtcaacgc tgagacccga atgataccag
    37561 gtaacccttc taacaaataa acacgtaaaa tgatgccgat tgggctcgtg cccatcgctt
    37621 ctgctgcttc gatcacccca ggatcaactt ctagcagtgc attctcgatt tgacgggcaa
    37681 aaaacgggac aacgccaaaa atcaaaggga ctaaagctgc tttggcacca atcgtcgttc
    37741 caactaagaa acgagtagtc aaagctaaaa gagaaagcaa gataatgaaa gggatggaac
    37801 gaatgatatt gatcaactta tcaagtattt cgaacagcag cctgttagct agtaatccat
    37861 ttggacgtgt cactactaac gtcacaccta agatgattcc gatgatccca ccaatgaatg
    37921 ccgtccaaaa agacatatat agtgtttgct ttgtcgcttc gatgaattca tccggaatca
    37981 aatagacatt cggcaaatat gttttaaaaa attcagtcat tttagtttcc tccttcgatg
    38041 actttgatac ggttatctaa ttcatttttt tgtaaaatag ttacttttac accagattta
    38101 atcaaataat ccaatgcttt ttgccgctgt ttggtttctc cagctaaagt aacgatcaaa
    38161 cttcctaagg gtgtttcttg taatagctct acattaccgt ataagatatt cgctgttact
    38221 tggtacttgc tgtaaagatg agcgattaga ggttcgttcg tctggttgcc gatataactt
    38281 aattccacca accatttatt tgcaatctga tcggcaaaag cagcatgggc actgattgtt
    38341 tccaatgctt ggtcaaggtg tgtcgctgtc cgaataaaat ctttagtcaa ttcttcttca
    38401 ggatgactga aaatctggat gctgctacct ttttcaatga ttttccccgc ttccataacc
    38461 gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt
    38521 agacctagct gacgatttag tttttttaac agtgcaagga tctgaagggt cgttttcgga
    38581 tcaagtgcac tagttgcttc atcgcataac aacactttag gttcatttgc cagagctcgt
    38641 gcgattgcca ctcgttgttt ttgccccccc gacagttgtt taggatacgc agatgctttt
    38701 tcttccagac cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgttttgat
    38761 tttccagaat actttagaga aaaatctacg ttgtcaaaaa tcgttcgact ttccattaga
    38821 ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc ttttggagag
    38881 agctggctta aattcgtctg attgacgatg acttctcctg ctgttggttt ctgcaacaaa
    38941 ttcatcacgc ggactaacgt actctttcct gcacctgagt aaccgacgat cccataaaca
    39001 tccccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt
    39061 ttgaaacata cagaaatatc tcgcaattca atcattaaca ttcctccttg tctataaaaa
    39121 aagcatcctc aagaaaatga aaatagtcat tttcttgagg acgccttttt gcgtgttacc
    39181 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtaccatca tactctagca
    39241 agatatcggt cgcagccgtc accgccttac atctatgctc gggatgaatt gctcgatggc
    39301 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
    39361 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaaatcc ttttactaac
    39421 ataaactatg tcagtaaaag gacgacgtat cgtggtacca ccttttttcg gaagtttcct
    39481 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacattt
    39541 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
    39601 gactcttcac accagccaga gcctctctgc aaagtttcca agcttacttt ccttctcaac
    39661 gaaatcaata ttttgttgtt gggaataatc tacaggagga aaactgaaaa gtcaagcatg
    39721 aaatccaaat tattttttct atatttattt ttctgttttt ttatctaaca tttcaataac
    39781 agttcgaaga tcttttcctt ccgcaagaag gttcaatttt tccttctctt cgacgctata
    39841 atttcgaaca aaactggaat cgggaatcaa aacaacgcct agtcctgcac gtgaagcggc
    39901 tgtcgctcct actaaagaat cttccactgc aaaagctgaa agagcttcta atcctgttcg
    39961 ttccaaagct gtcagataag ggactggtga aggcttgttc tccgatactt catctccgaa
    40021 cgtaaacgta tcaaagtaat cggctaattg aaagtgatcc aagatatctg ttgctctttt
    40081 cgtaacagtt gtcgtagcaa tccctagttt catatgacgt tcttttaagt ctgtcaatat
    40141 ttcttttgca tagggtttca attggatagc tccacttgtc agctgttgat aaaaatattc
    40201 ttcacgcttt gctcggactt cctgcacttt ttcaggactg cctgctgcct gcactaaaat
    40261 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt tcgggatatg
    40321 aatatcaaat tgctggaatc cccaaagcca accatcccga tacgttcttt ccgtatccac
    40381 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
    40441 aatacaccta gtttagcatg actgatccaa aatgaacaga agcatagagc ccagccgcat
    40501 cacttataag agttcttgga atcgacggat atagattttc ttcacagctg tcacaagcgc
    40561 cagataagcg acgactgtta taaacaacca taaccagtaa ttgccaggga gcggcatcaa
    40621 gcctaactca gcaccaaatc ctgtgaatgg aaggactgaa ccgacaatga ttcctaatgt
    40681 agtgataacc agcatggcaa atgttgcatt gctttggata aatggtattt tcggtgtacg
    40741 caatgcatgg agtactaatg tttgtgtcca cagggattcg acaaaccatc ctgcatggaa
    40801 taatgcgata aaaatcactt gttgtgagct atccaatgta tggaaacttc caccaactac
    40861 agcaggacaa ataacaaaat acatcaacaa gtaagtagtg atatcaaaaa tcgaacttgt
    40921 tggaccaaaa taaaccatga attttccaat actagatgca ttccatttct ttggctcatc
    40981 caaatattct ttatccatcc gatcccatgg taaggaaatg caagaaacgt catagatcag
    41041 attcaaaaat agaagttgca aaggcaacat tggtaaaaat ggtaagaacg tactggcaat
    41101 cagtacggaa aacatattgc caaaattaga acttgctgtt gccttgatat atttcatgat
    41161 gtttccaaaa gtttctcttc cagaaagaag gcctctttcc aaaatcgtca aatctttttc
    41221 taaaaggatc acgtctgctg attcttttgc gatatccaca gctgtatcca cggaaatacc
    41281 gatatcagcc actttcattg ctggagcatc attgatacca tctcctaaaa atccgacagt
    41341 atgcccattt ttacgtaaag ctgtcgtcaa tcgtgctttt tgctgaggat tcaattttac
    41401 gaatacttcg tgttcttcag ctacttgcgc taattcatga tcattcattt ccgagatttt
    41461 ctcaccagta atcaattcat tgatttctag gcctacttga cggcacacag accgtgtgac
    41521 taattcattg tccccagtca gaacttttac agctacacca tgttctttta aagctttcaa
    41581 cgcttgtttt gttgtttctt ttggtggatc aaggaaagca agataaccaa tcagaaccat
    41641 gtctgactca tctttgactg aaaattctcc aacaacgctt ggattggtct tttgtgcgac
    41701 accaatcact cgtaagccgt cttcattaag tgattgtact tttgcaagca ccgcttcttt
    41761 tcgttctttt gttaatggtg cgacagtacc accaagatca ataaatttag aaatgttcaa
    41821 catttcttca atcgcgcctt ttgtgatcat ctgtgttttg ccagccggat cagcgactac
    41881 aacactcata cggcgacgtt caaaatcaaa aggaatttca tctacttttt gataattgat
    41941 tgtatctgta tctaaagttt ctgctgcttc atcaatgata gccacatcta acaaattttt
    42001 cagccctgtt tgatagtagc tgtttagata agcatggcgc aaaacacgat catcttcatt
    42061 tccttcacaa tccatatgat attcaagaat gattttgtct tgtgtcaatg ttcccgtttt
    42121 atccgtacat aacacatcga tcgcaccaaa attctggata gcatttaaat ttttgatgac
    42181 tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg
    42241 aagcatttca ggcgttaaac caactgccac agataatcca aaaagaaaag cttctaacca
    42301 atctcctttt gtaagcccat tgataacgat aactgctgga gccatcaatg acataaattt
    42361 gatcagcagc atggatgttt tgtggatgcc taattcaaaa cttgattgga tgggtttttc
    42421 agatagcgtt tctgctacat gcccaaatag tgtgtcattt ccgacagaaa caatgattcc
    42481 ttccgcgctt ccactgatga cattgcttcc cataaagaca agattttcat aacttgtttc
    42541 actttgctga tcgtcttcac atactttcgc atgcttttct actggatagc tttcccctgt
    42601 caatgctgct tgagaaatga atagatcctt tgcggacaat aaacgcatat ccgctggtat
    42661 catatctcct gctgaaagcc gaacaagatc tccacagacg atttcttcca taggaatctc
    42721 tgtatactcg ccatttcgtt tgacagctgc cgttactttc acaagtgatt tcagtttttc
    42781 agctgcttga ttagaacgca cagactggat caacgtcatc gtcccactga cgataaccat
    42841 agtgaagata atggctacac caaagagatc tttatctcct ggagcggcta atacgtattc
    42901 cgtaatgaat gaaataattc ctaatgcaat caacactaat gtaaagggtg tgatgtatgc
    42961 tttcaatatt tctataatga atggtgtttt tttgccatat gaaatcgcat tttcaccata
    43021 agtttctctt gaacgctcga tttgttcttc tgacaatcca tttggtgatg catggaacaa
    43081 ttcaaataat tcttgcttcg tttttccagc aaaagctgca tagatttttt gtgtatcttt
    43141 ctttttttcc atctcgatct ctcctttgtg tccaaaagac agctgttctc gctgtttttg
    43201 gtcttttgct attcatcgga tcgatagaga aaatgctgaa aggggctgag aacagataga
    43261 taatagccct ggcctttcct ctaaatcctt gtttttcgaa tgactgtcat ccatttgttc
    43321 tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg aatagttatt
    43381 ttcccaactc gctcacctcc ctttttgttt tttcacctat gggatttgcg gcaacaaaaa
    43441 aacctctcgc caaatactca gcgaaaggtt tgaaaaagca aatcaaaaaa agcttctcag
    43501 atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatatctgc ttttagctac
    43561 cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt
    43621 tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcctattc ggttttccct
    43681 cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt
    43741 tttaaaatac acgcgttata tttcctgtct cacgcagttt tatttgtctc attttccacc
    43801 tcccgctata atagaattat agtaggaaac gaggtgctgt tatggaacga acacttgtta
    43861 ttatcaagcc agacggcgta agacgccatt tggtcggaag catcatccaa cgttttgaag
    43921 caaagggact ggccattgct gaaatgaaat ttgcaacgat gacacctgaa ctagcaaaag
    43981 aacattatca gcacttgact gaacgtagtt tttttgatga gcttatcgat tacatgactt
    44041 ctggtccggt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga
    44101 tggtcggagc aacaaaaggg gcggatgccg ttccaggtac gatccgagga gattatgcac
    44161 tgccggggac tgaaaacatc atccatgctt ctgattcccg tgatgctgca acaaaagaga
    44221 tcgcccgctt tttcccgcca ttcgatacaa acaaaccgtc tgaaaaagcg gcgaagtgat
    44281 acaaatccta aactgttggt ctaactgctg ggtttagcaa tggccagcag ttttttctct
    44341 aaagaattcc tttgattctt ccctaatgta tattcgatca ctgcgaaaaa ctcttggtga
    44401 ttttccgatt tttcttcccc taaagcagca gaaatcagtc gttggtaata atccgccatc
    44461 aatgtttcat aaatcactgt acttctttct ttatgcgcta aactccgttt catatcttca
    44521 ttcacgctac gtgccttttt taagcgacca ttttctatat aaaacaaagt caagttcgtc
    44581 aaaaaagtaa agagatactg ctcgtgatta ttcagttgcg cgatccgtcg cattcttttt
    44641 ttgattttca aataattatt ctcaataaaa cgatcactga atatccaagg aaaatctgct
    44701 agaatagata cttcaaattg cccatactct ggtacttgca tcaaatattc ttcaatgata
    44761 cgacattcat aacgcaaaaa ttcaaattcc gtctcagata cttctgtttt tttggacatg
    44821 tgcagattca aaaccaaata gtacagatct tctgttttct tatagtgcct gcggttatta
    44881 gcaatttctt tgtaaggatc aaagctgtct tcttgaatcc gatctagcat tcgtcttttt
    44941 ttctttaatt tttttggctc atcaagctga tttcgtatcc attcatattc ggcaagtgtc
    45001 atgttcattc gctctaaaaa tgatttcaat gttccaaacg atactttttt cactgaattt
    45061 gtctcgatcc cgactagcga agttttagaa cagattcctt ccgctaattg ggtctgtgtc
    45121 agctttttgt tgactcgaag cttcttaatc aattctccat catacataat agttctcctt
    45181 ttttaattga aattaaccct tggcttttcc agaatgcttg ccaataagag gaatgattat
    45241 ttcaatttaa attttccgca ttcaataaat agatacgtta aaaataaaaa atacacgaaa
    45301 aaaataaaaa aatgtttttc tcataaaaaa taccaacctt caagcatgag actgctgtct
    45361 aatccctgaa ggttggtttg attgtttatt gatcattttt ttactgctcc gcattggttc
    45421 ctgttactgg atataggata gtcgttgttt tattgccgtt ttttgaaaag acactggtcg
    45481 aatgttcacc tttttgcttt tcgattgctt cattacgttc cgcttcgttt ttatagtcat
    45541 attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag agattttcct
    45601 gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct ttggagattt
    45661 cctcgtcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt
    45721 ttcctcctag gacggtgtat ttgggcgtca cgaagttccc atttcgaaaa gcaactactt
    45781 gatcgtgctg agaagataat aagtctgtac caaaatgaag ataatcttta tcatcgattc
    45841 ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accatattca tgattgactg
    45901 tccctttagt gtaaccaggc atatggatca tcaatggcac tcgttgcatc tgtgcgttgt
    45961 caaattcatc ccatgtatct ggatctttac caagagcact tgctaaatct ttattatctg
    46021 tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg taaataccag
    46081 attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgg gccgttcgaa
    46141 cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat
    46201 ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta
    46261 gatgttcaag atattgggca gattctctga ataaatcttt atctaatatc ccataaccta
    46321 acgtttcatc ggattcatca aaataagatc gatcaaagaa attgtcatac cctaaatttt
    46381 tatatacatg atctctattc cagaaactag ctacattccc atggaaaaca gcacttgtat
    46441 agccttctgt ttgaccaagg gtcgctggtg ctgcttgaaa gacattatct gacccaagtt
    46501 ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacatg ttttcagcat
    46561 cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaaactgatc gttgcttgat
    46621 tctggaaaat actatttaaa aacggcgtga cttcttgtcc gtctaccttc atattgatca
    46681 aaaactgttg aaaactttct aaatgaagga caatcacatt ttttccttta gcaatcccaa
    46741 atgtttcagg atttggctct gcataatgtt tctttgtata gtctaaaact tcatcaattc
    46801 cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt ccgtcataaa
    46861 tagtgtacgc atcgattcct agatatttga caatatagga acggtcaaat gtccgctgta
    46921 gcaattgggg acggttggct tcgctcaacg ctaaatttcc tgaaaaaatc aatcctgaaa
    46981 gacaggtaac agcgatagcc atcggtttgc tgacagggtt gcttttgata ggtattttct
    47041 tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccaaccaa tagatgacat
    47101 catggggttt catcaaagaa aaggagcttc ctgacaaccc ttgtgacact ttcgaaaagc
    47161 ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata atattcaagt
    47221 acaacaaaag cgtatttaaa atatcaatga tcaaaagaat ggctaacgct ggctttggct
    47281 ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaatcggg ttgatccaca
    47341 agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag taggcaatga
    47401 tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga ttttggcaca
    47461 attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc ttattttgct
    47521 cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta ttctttctaa
    47581 agattattcc attcattttt cacagcataa aatcgtttcg gtgtaagcgg ttaaaatagt
    47641 aaggagataa aaatcttgtg gtgaccgatt tactctatag tattcaaaag tagtttctcg
    47701 gtattcgtac aaaaaaaaca tactaagatt agaggggaac atcattgacg aatgatgttc
    47761 tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga acaaagcctt
    47821 gaggactacg agtccctatc aaaaaccgaa cttctttact gtcattttag aatcaggttt
    47881 acgtatgttg gggccatgag cccgtgtata ggaaattgga atcgaaatga agcagtaaag
    47941 ttctttcaac acttaaaaag gaggaaaaca aatgccttac attcttgcat tagatgtttc
    48001 aatgggacac agctacagtg tcctatacaa agacgacacc tgcttatttg aagacgagat
    48061 cgaacacact caaagaggat ttcatgcatt actcgaagag atccagcact tgcctgaacg
    48121 tccattgatt gtttttgaag caacgggtat ttattccaga ccagtcgaaa agttctttca
    48181 agatcatcac ttttcttact gtttgttaaa cccgttagaa gcgaaaaaac agatggaaga
    48241 aaacacctta cgcagttgga aaacagataa atcggatgcc catcggctcg cacagactca
    48301 tcttaaaaat caacgtcaac caaaagaagt acaaagcaat ttttacttag agatgcgcga
    48361 tttagcccgt ttttatcaag aaattgagaa gaaaatcact cgactgcgca tggatttaca
    48421 caactgcctt cagttgactt ttccagagct tgaacagttc ttttcaaaca ggctgacccc
    48481 ttacgcattg acattgatcc gtctttttcc ccatccagat tttgtattgg catctactcg
    48541 gacaaaaata aaaaataaac tgatcaacga aacacgggaa aaaatctccg cgaatcgtgc
    48601 cgagcaaaag gcagatcaaa tcatccacta tgcacagtgt gcctatccag ctgttgaaaa
    48661 agacagtatt cactgtcaaa aaacgatcta ttacgccgaa cttctccaag atttactcga
    48721 acaaaaaaaa gcactggcga cacaaatgat caaaaaagcc gaagcctctc cttgcttttt
    48781 gttgtaccaa acgttccctg ggattggcgc actcacagcc gctttattgc tcggtgaatt
    48841 aggagatatc acacgattta aaacccataa acaattgaat gcctttgtcg gaatcgacat
    48901 ccgacggtat cattcaggta agtatactgg ccaagaccgc atcaataagc gtggtaatcc
    48961 caaagcacgg aaaattattt tcttcaccat tcgaaatatg attcgccagc aacgcgcagc
    49021 tcctaatcat attgtcgatt attactacaa attaaaaaag caacctatcc ctaagaaaga
    49081 taaggttgcc acagttgctt gtatgaacaa gcttctgaaa tgtatgcacg ctatggttag
    49141 ggcacataca gagtacgact atgcgtacgc ggtctcagtg gaccattaac taaaccactt
    49201 acagtatacc acttctttgc ccagatcgaa aattttttac gaacggggct ttatttagtg
    49261 tgccttgatt ttgtcgcttt cgtttacgag ctatttttct tgcacctttt acttgactaa
    49321 tcgtaggaaa gaggctgtga caaaattctt gtcgcagctc cctatttctt gatgcaggac
    49381 agctttttca caacctctcc actaaacggt gttcaatcag ctgaccttcg gtattagctc
    49441 aaaaaacggc aaaatatgag gagctatttt cccgtttttt tctctaatac tcaggtcaaa
    49501 ccgctgattt cacaacctct gattcacggt gttccagaag tagcttcttc ggaaataagc
    49561 cgaaatttta caaaaacttg agaagcactt ttcgtaaatt tcttcttatt tcttgaagct
    49621 gaccacttct gtcacaactt ctgtttttca ttgtccatag gtttgatatt gtccttctga
    49681 tgactcattc gtaatgattg aggcttgatt ttcttcattt cccgttatcg tttgtccact
    49741 gccagaatct gaagggctta gttctgtcga ctcgtttttt tgagaagaag cagttgtttt
    49801 cttgacttgt tcgttggtct ttttccattc cgctagtcga tcggccacca cttttttgtc
    49861 cactttttca gaataagctg taaccacttt tccatttttc aaacgtgcta ccttgtcgcc
    49921 atcatgagtc aaaagcaaaa cgaccaaatg tttgtctttg aattgatatt gtctgagaga
    49981 aatcgcacta agtacgtcat cgatattgat aaattgattt tcttcttttc cttttggcaa
    50041 atagatgtcc aattcacctt tttcgtaatt ggcagttaat ttatattcct cccaccaata
    50101 agttgagaat tcttttttga aattatctac tgattctttg cttgctacga ttttttctgt
    50161 ttctttctga gcaagatctg ctgtcatttt tcctgaaaat gctgcggata aaataagcag
    50221 taagaaaaaa ccgaataacc aaaagagaag aaacggtctt tttttcctca tgccttcctc
    50281 cactcctatt taattttgac tattctatta tatacaaaaa atgaaataga aagacaaaaa
    50341 agttgaaaaa gatcgtaaat aattgaactt ctttttcctg aagcggtaag aacgggaatt
    50401 ttcatacatt ctctcttggc tattctttaa gcttcaaatc catttctgtc tgatacatct
    50461 ttgaaccaat gacccgattt cttgatcgtt ctttttccat cttgtgctaa atctactgaa
    50521 ataaaaccgt aacgattctt ataggcattg gtccacgacc agttatccat gcatgtccac
    50581 atatgataac ctaaacaatt ggcaccttct tgaatggctt gatggacata tttcagatga
    50641 gattgaacaa attcgatccg ataatcatcg tggatcatac catcttcctt gataaatcgt
    50701 gcttcatttt ctacacccat gccgttttca gaaataaaac agtcgatatt cccataatga
    50761 tctcgtacat tgatcaaaat atcatagatt cctttttcat agatctccca tccgcgataa
    50821 ggattcattt tacgaccagg catttcataa ttgtcgaagt aatcctcagg cataggaccg
    50881 tttttagtat cgattggtgt ttcttttgct tttattcggc gtggttggta ataattaatt
    50941 cccaaaagat ctactgtgtt ttctttaatg atcgtcaaat catctggatg aatctccggc
    51001 attagatcga gttcttttat tctctcaatc aattcactgg gaaactcgcc ttttacagct
    51061 ggatcaagga atgaacgatt gaagaacgca tctgctattc gtgctgcttt aacatcttcg
    51121 ggatttgatt cgtctcttgg ataactcggt gtcaaattta agatgattcc gatcacaccg
    51181 ctttgatcca tttcatggta agctttgatg gctaatgctg aagccaacgc ttcatgataa
    51241 ccaacttgga ttgccttttt catatctgat tcttccggat aatgccatcg atacaaatag
    51301 cctgcctcga ctggaacgat tggttcatta tgggtaaacc atttttttac tttcgagcca
    51361 aacaattgaa aacaactttt tgcgtatttt tcataggctt caaccgtttc tcggttcaac
    51421 catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt aacaaagggt
    51481 tcgataccgt tttcaatcaa ctcatcaaaa tagtgatgat agaaacgaac ggcttcttcg
    51541 ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagaggt tcgaaatgaa
    51601 ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata aacttgcgag
    51661 gttttttcag gtcctacttg atcaaagaac ttttgcggtt cgtttttgta ccaataatca
    51721 aaaatattct ctccttttcc atcgcctgaa attcttcctt ctgtttgtgg accacttgct
    51781 gcagagcccc accaaaaatt ttttggaaat tgatatcttt tcatcaaagc acctcttttt
    51841 cttttttagt tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc
    51901 tcttcttttt tgattcgttt caacagctgt tctgctgctc gttccccgat tttatctgtt
    51961 ggtggcgtga tcgtgatttt tggaaggata ctcagatcgt ttttatctaa ataatcatag
    52021 tgactgaacg caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca
    52081 ccaatcgaca tgttgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct
    52141 tttgccccgt aatagccgct ttttctagaa tagtctcctg taaagatctc taaatggata
    52201 gacggatcct tttgtaccgc ttctttggct ccagctaaac gttctcttgc tacgtaatct
    52261 gtttgagcgg cagcaataaa tcctattgtt ttatgtcctt gtccgccatg atgagcaatc
    52321 acttttgcta cactttctcg atcattcact actattgtat caatacctgc tggcccaccc
    52381 ggacaattca atgcgacaac tgggatactc atttttgtca gcatttcaaa tccagaccac
    52441 tcttcttctg ataagaagac gatcaatcca tcgatcgcat gcttcatcag gtattcgatt
    52501 ttttgttcga ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt
    52561 tcttcagcta ctctttcgat ccctgaaaca acctccgaac caaatcggtt agatatcccg
    52621 tttatcaaca gtccaataga gaaacttcga ttattcttca atcctttagc aatgatgttt
    52681 tctttatagt ccaacgctga aatcgcttct gatatttttt ccatattctt ttccttcagc
    52741 tgctgaccgt ttaaatagcg ggaaactgtt cccactgaaa caccagccat cttagccacg
    52801 tcacggatcg atgccataca ttttcactcc tgccagttac atttgatttc tttttcttgt
    52861 tgttcgacca tgatgaccgc tttctttgca taatgccgct tgtcatcaat atccatataa
    52921 ctaaagtctt cagaagtgag ttcaaaaaca agcagtcttt cctcttgaga ccgcaattca
    52981 tctttacgaa aagctttcaa taatttcgtt ctttggatga caacgccgtc aattaatcgg
    53041 acatagacaa gagtacttgt tttacctgaa acacttcctg tatttttaac tttaattgat
    53101 attattactt tgtcaatatc cgttttgact tccatctgac tgtattcaaa tgttgtatag
    53161 cttagaccat gtccaaaagg ataaaatgga cttccgctta aatcataata attctcttgt
    53221 ttagaagcag cacgctgata gtaataaaca ggtagttgcc gactatttct aggataagac
    53281 aaactcaatc gtccgcttgg gttatttctt ccagaaatca tctgcgcaat ggcttttcca
    53341 ccctcctgtc caggaaacca tgctgttgca atggcattcg taaatgactc gattttcatc
    53401 aaatcgtagg gtcttccttg cacaagtatc gtgatgattg gcttgcctag ttgatacaat
    53461 tgttcaatca gctgttcttg gtatccacct aaagaaagag aagcaacatc tacattttct
    53521 ccagaatcca tgttgatccc tttcgatgaa actgccccgt tacgaagaaa ttccatatca
    53581 aagttccgcg cacttgatcc acctagaact gcaatgatca catctgagtt ttgagcaagg
    53641 gtcaccgctt ctaataattt ttcttcctga tcctcctgat ttcttacttc acatccttgt
    53701 gcataagaaa cctttgattg gggaaactct gtttttatct cttgtactat cttttttttc
    53761 aaacggtctt ctgtttgtgg tgcagagtag tctcccaaca agtggtaaac atcgtgagca
    53821 tttggaccta taacagcaat gttttttact tgttgatcca acggcaatat accgtcattt
    53881 tttagtaaag tgatactttc ttttgctgtc tctaaattta agtgctctga ttcctccatg
    53941 agatgatccc aatactcctt tggatcgttt gcaaatggct gttcaaataa acctaataaa
    54001 aatttgacag ataaaactcg gcgaactgct tgatctagta gcttttcatc tacgacttga
    54061 ttatcaatgg cttctgcaat cttggtatag gtctcatccc acaaactgag atcgattcct
    54121 gccgctaatg catatgctgc agcagtcttt ttatctgtaa aaacatcgga taaacgatcc
    54181 aaagccacac cgtctgccat aacgattcct tgataaccga tctcttttct taatgtttgc
    54241 tcaaaaagcg ctcgattgat atgacaaggt atcccgtcga tgtcattata agcagccatg
    54301 acacctactg catttcttgt actcttcaat aatggtccgt aaatatcgga gaactctctg
    54361 cgtccaatcg ttactgtgcc tgaattatga ccgcctaaag cttctccttg tgcgatacag
    54421 tgctttaaca caacaccgat ctgatccttc ttcttattga tttcctctac tgtctgatca
    54481 agaaaagaaa ctttttctga aataatctct ccttggaacc ctcgaacgac cgcttcagaa
    54541 aaacgagctg agagaatcgg atcttcccca aagcactctt ctgtccttcc ccaacgagga
    54601 tcttttgcca gatccaaagt cgacaccagc gctaaatgaa cacctttagc agccagttct
    54661 tttgccatca atcgactcga ttgttctaat aatgctacat tgaaagtatt tccacggccg
    54721 atattcgttg gataagaaat cccatccaag ccttgatgac catgtggaca ttcttctaca
    54781 agcaaaactg gaattttcca tcgcgaatgc tgtagtacat attcttggac taggttagcc
    54841 accttccagc tttcttttgc aggaatccca ttcttataat ctgttttaga ccacggatca
    54901 gagcgaaaca gaccgtataa ggcacccaga ccgcctcccc atttaacatg ttcttttaac
    54961 ttttctgtta attcgatctt gtctccgttt ttttcataac attcccaacc atacaaatgc
    55021 tggtttacct gtcctacttt ttcagcaata ttcatcctcg aaagtaaatc ttctactctt
    55081 tggcggatgg ttaactttgg gttttgatac gggtaatcgt acaccttaat atcctccttc
    55141 tgccaatttt gccttattag cggctaatac aaatggcagg aataaaaata catctagaat
    55201 gataagtatt actgttaaga ctgctgctcg tatatcgcct cctgtagcaa taaaagattg
    55261 tataaccggt ggagtgatcc aaggtgcagc agcaactgtt ttactgatga ttccaagact
    55321 tgtggctgcg taggcaataa ccaagttaat agatggtata atagcgcatg gaatcatata
    55381 tattggattc atgactactg gtaatccaaa cataattggt tccgtaatat taaataaaca
    55441 agtagtcaga ccaaatttag cgattgatcg ctgttcttga cgtttagaag cccacaagat
    55501 agcaataact aacggtaaaa tacacccgcc acctccgatc aatccaaatg cattggtaaa
    55561 gggctccgta ataatattag gaatttcttt tcctactgaa aaagcatcca tgttttcctg
    55621 aatagactgt aacaaaggtg cttgccgtat aggagccaaa atactcgaac catgcatacc
    55681 aaacacccat aagaaatcag agaaaaactg taaaaataag atgcctggga cggataaaac
    55741 aaaagctttc aatggtgtct gaatcgctaa ttcaattaat tgcggaacat tcaaaccagt
    55801 cgtatggttt aaagtaaaaa cgacaactga aaaaaataaa atgacaacta tttctggtac
    55861 taaagaatta aatgacgcag ctactgccgg tggaactcct tctggcatcc tgattttaaa
    55921 actcttcatt ttcgctaatt tgataaataa ttctgtactg acaagggcag caatcatcgc
    55981 cgttgctaag ccagacgaag aagtcagact ttgtaagaaa acccctgtag cttctgctgt
    56041 atttccgtcc atcaaagtga ctgtacttga taaagacatc aaaataaaca ttacggctgt
    56101 acttagaatt cctgcatgaa caggactaaa tgatggatca tcaatcacgc ctgtttggat
    56161 atagtgggtt gccaaattcc agccgatatt gtaacacacg ataatagcta aaatactcat
    56221 cgtaccatta ttcacgattg aagcaagttc agccaatgga cttagatcaa gtattctttg
    56281 gactgttgca tttgaaaaaa tcagagcatt cagcaaaacc atgacagaag cagccattac
    56341 taacggcata gtaacgataa aactgtctcg aatggccagc aagtgtcgct ggctactcat
    56401 cttagatacg attggtagca ttttttcaga tagtctgtct acgactttca ttatttcatc
    56461 tcctagattc taaaattgaa ccggttcatt tttaatatag cacttttttt aaagattgca
    56521 acacattttt ttaaaacgct ttctaaaggt ggtggatttt ttatgacttt tgaaaaatta
    56581 ttctatttaa tggtattttg tgaatttttt ctcttccatc cgtttgaagt tttactttcc
    56641 aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa ttaatcgtac
    56701 tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt ggcggctctg
    56761 accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa gcagatatca
    56821 ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg ggacagttga
    56881 catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga gctgattctt
    56941 ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gattactacc aaagatgacg
    57001 aatatattat ttcaggaaaa gaaaataaaa aagaagcaac agtcacattt accaaaatcg
    57061 gtaattaccg tttgaaggat aaggatggca acatctacag tctatagtgc catctgttgg
    57121 atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat gactgtaaag
    57181 gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa caaaaatatc
    57241 catacagaat ttaatgatca aggaagtttg agtatatgcg acaattgcca caaactagtc
    57301 aaagattctt ttaaatcgtt tgaatcttat cctgtcaaaa attatgtaaa aagcaaaaga
    57361 agataagcaa aatcaagcaa aactcactat ggctgatttt tatgaacttc aaactcagct
    57421 tcttcctttg atcaagcttc aaaaactgga atcagtaaaa caaacagtgg atcatttcag
    57481 ttcacaaatc aaagagtaac gttttattta tttcacatct ttgaatataa aataaccaaa
    57541 cgatagaaag acttattcta catcacgtag taatttttct ttcccgtcgt ttggtttttt
    57601 acttttagcc taattcttga cctttcgcat gctgattatc tgtaaaatat tttccagcag
    57661 gctttccttc caattcagct aatttttctt ggatctgacg aatcaccttt tttgcatctt
    57721 ctggattttc tacaaaatcg aatgtatccc catccacttg tattttagga catacatcaa
    57781 aatcctcata ccattgatta tagcgggtgt tcagtgtttc gtaataggtg tataacgttt
    57841 catcgctttc tagctgttca tagtcacgcc cgcgtttttt gatacgttct agcatcgtgt
    57901 ccaaagaaac tttgatatgg accatcaaat caggacgttt ttttggagca acatcatcta
    57961 actcgtttag catcgtatct aataagctat cgtactgctg tacttcgata tccgttaccc
    58021 gacctaaatc agcatttaga tggaacaaca gactatcttc gtagatggag cggtctagca
    58081 cattgtcatc gttcactaaa gcatttttca ttgctaaaaa tcgcttattc aaaaagaaga
    58141 tttgcaataa aaatgcgtac tgctcaggat ttgaataaaa aagcggcaat acttcgttat
    58201 cttcgacatt ttcataaaat ggtttagaat caatctcttt tgcaagcata tccgtcaaac
    58261 tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa cccttcctct
    58321 tctataagta ctcgcctacg taagtttcaa caggcaattt ttcaaaatca tattcacgta
    58381 cagcttggtc tgattttaaa tacgtgtgaa gactttgtgt aatcatcaag ttcgtaggta
    58441 ccgtctcttc ttgtacgatg atcatcggtt ttttcaacat agttgccaca cctaattcgt
    58501 aagccgttcc tggatcgatt gtttgatgct caaagtcaag gatagccaaa acaaattctg
    58561 catttgtcaa ctcttccata tctttttcgt atacttcctg cgcccatcca gcgctgaata
    58621 gttcataatt gctttcttgg tgatgacgtg gagagtagaa acttgtgact gttttgtttt
    58681 cctccaacgc tttttcgata cgacttactc gatcgatttg ctcctctgaa aaaaatggtc
    58741 ctgctaaata tatttgactc attattttgc ctccttcaat aataactgat ttttaataga
    58801 tactgctcgt aattcattga ccagttccaa tggattgttg gaaaagatca cttgatccaa
    58861 ccatttttta tccataaaat cctcttttgc aaaagtttct aataattgtt gcaagccgct
    58921 gtaataaccg ccaatattca atattccgat tggcttatca ggtgtcaggc cgatcgctat
    58981 ccaagaaaga agttgcgaaa attcctccaa tgtacctaga cctccaggga aaacgaggac
    59041 agcatctcct ttttcaaata ataatacctt tctttcatca attgtctctg taggaataaa
    59101 tgtagtaaca tctttcccag gcagctcagt ttcaaaaagg ccatttggat agatacctgt
    59161 gacttttccg ttattttcca gtactgtttt actaatcact cccataatcc cggagtatga
    59221 accaccgtaa accagttcga tttcttcttg tgccatatat tttcctaacg tttgcgcaat
    59281 aaatttgtaa ctttctttat tgccaaaacg ggaaccgcaa aaaacagtaa gtttcataaa
    59341 aaaacactcc ctaactcaaa agtctatctt cattctacac gtgttttttt aaaataaaag
    59401 actagatttt gtagtttttt agtggtatat agatcaaaca ccactatata tagtatttgc
    59461 aaaaaaataa tttttcagct attttacagt aaccaaaaaa ataaataact agtaaatata
    59521 aaattgaaac gacttactta tagaagaaat tgggatttcg tcctgcatgt caataacacg
    59581 cattatttaa atagtcacac acttgtttct cctattaatg aaaaatccat tttattctcg
    59641 tgttatgttt caccaacgcc tgatgtttct ctaaataaaa aagccgacga accaaataat
    59701 cgaaatttga tcattttggt tcatcagctt gttcccaaac ctttgtatta acgataaata
    59761 gctggattcg taaaactatc acttggatca tcactgctgg aagaaaattc ggtgataacc
    59821 gcgcctgttg cacctgcctg gaaccagtgt ttaatatttt gttcgatcgt gtactgatct
    59881 ccagcgtgaa gtatgatttc tttttttgcc gtatagtaac gttcttgccc ctttggaata
    59941 atacaggaaa gtttttttgt ttcttttcct tcgacataaa gatatactaa gccttttcga
    60001 caacgaaagg tttccttttt tccttcattc tcatctcgag gtgggtgccg atgctctgga
    60061 caagtttgat aaggcaataa taccagttct ttcccacagt atcgctcatt attttcataa
    60121 acaaataaat tcaatccttc agtctcgatt gctcctaatc caaaatctgt atattcgatt
    60181 ctttctaatt ctgtttcttt caatactact cctgaatcta ggaacatttg ttgtactctt
    60241 ttcttgtaat ccattctgtt cctcctaact gattgtcaag tcatcccaag atatctcttc
    60301 ttcctctttt tcttcttgga tgacttctgc atttgggtca actggttttt tcaataataa
    60361 aatagttatt cccgtagcaa aagaaccagc taacaatgca ccaatgaatg cccatggtct
    60421 ggtcatcgta gcgactgcaa acattcccga tgccggaata cggctatctg cgccccaaaa
    60481 catgctcaat ccaccgccga ctgccccacc gactcccgtt gctatgacat tgcgtgccaa
    60541 gtcgttcaat acgataggga aacatccctc tgtgatctgg cagatcccca taggaaaagc
    60601 ggtcttcaat gtatcgattt cttgacgatt gaatatattt ttacgaatga ttttccctaa
    60661 gaaataagcg atcgtcaagc cgaatggggt taccatcgaa gctaaaatca atacagtgat
    60721 tggttcatag attccttctg aaaatcgtgc cacaccccct tatccagaat aaatggtaat
    60781 ccagaaacag ccgggtatct gcggtaataa gcctaaccaa acgaaaaata tgagaagcta
    60841 atatgagaag ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
    60901 aacctctttt tttatttatg tttcataatc aatatggtaa ggatgcagct ccgtatggta
    60961 gaccgcctcc gaatattcaa tcatttcccc aatttgcgta gacgatttcc gtatccgttt
    61021 gatgccgatc tgtgcgtctg ttttcagaat ttcttgttct tcttctgtta aaaatactgc
    61081 cgagaaatca tcagaaaatg agtcaatagt atatcctttt ttcgtcagca aacgatacaa
    61141 agattctttg ccaaattctt ctctgctgaa ttgcatcatt tcttgtggaa gataatgaac
    61201 aaaataaata taaggctgat tgttcaaata atataagcgc ttaaaacaaa cagcttgctc
    61261 accgaaaaag gaaaacaaag gatcttcctt ctttatatgg accaattgca aatccagcgt
    61321 ttctttccgg acttcatatc gggagttctc caagatttgc gtaaagcttg ccgctttgga
    61381 taatcgattg taaggacgat tgcttagaac cgtcgtccca cgaccacttt tctttgcgac
    61441 atattcatct gatgccagca gttcgattgc ctttcggaca gtgattttgc tgacttgaaa
    61501 cagttcttct aattcacttt ctgtcggtaa catcgaaccg actggatatt ttcctgagaa
    61561 aatatcttct ttaattttat ctgcgacttc caagtatagt acacgtgtcc ttttcataac
    61621 gtcacccttt gctttactat tactaaaaga atagcacata aaaaataaaa aaagaacaca
    61681 aatcacatgt gagaagtgtg ttctttttca ttcagctgct ttaattacaa gtttacgcca
    61741 tttgaggcaa ttacttcttt ataccaatca aaagattttt tctttgatcg attcagtgtc
    61801 ccatttcctt catcgtctag atccacatag ataaagccgt aacgtttact catttctcct
    61861 gttgatgcac tgacaagatc catacatccc caaggcgtat agcccatcaa atctacaccg
    61921 tctgctactg cattagccat cgcttcgata tgacgacgta aataatcgat ccgataatca
    61981 tcaataatcg tgccatcctc ttccatatga tcaatggctc caagaccgtt ttcaacaatg
    62041 aataatggtt tctggtaacg attgtataat tcattcaatg cgattctcag cccttctgga
    62101 tcgatctgcc agccccattc acttgcctca agaaacggat tttttactcc tcccagcaag
    62161 ttcccttctg aagttgctgc ttgtggatca gtctcattga ttgccgtaga catataataa
    62221 ctaaatccga tgtagtccac tggatattct tttagcaact ggaagtcttt ttccgtttgc
    62281 tctaattttt ccggatcgac tccatatttt ttatgggtac gcgctgtgta tgcagggtat
    62341 tctccacgta cttggacatc cgtgcagaaa aaattgaatt cttggttttg gatcattgtt
    62401 gctgcctgat tgactggatt ggcatcgatg ctgtaagttg ttgcatagat aatcatgcag
    62461 ccgatttgga tatccgaacg caattcgtgg ccgattttta cggctttgct acttgcaacg
    62521 aattgattat gccatgcttg gaaaatattt tggtactctc ctgctccatt gctttttact
    62581 aatccttgac ttaaagctgg aaagtgaaag gccgaattga tttcattgaa agtcatccaa
    62641 tatttgactt tactgctgta tctctctaat actgtctgag caaatcgttc atagaagtcg
    62701 atcagttttc gatttttcca gccaccgtat tcttttgcca aatgaagagg catttcgtaa
    62761 tgagaaatcg tgatcacagg ttcaatatca tattttaagc actcatcaat cagctgatca
    62821 taaaattcta atcccgcttc gttaggagta ccttcgtctc catttggaaa aattcgtgtc
    62881 caggcaatcg aaaaacgata acatttgaat cccatttccg caaacaaggc aagatcttct
    62941 ttgaaacgat cgtaatgatc aattcctcga tgattcgggt aaatatattt ttcttgatca
    63001 atcgtccagt caaattcttc actgccgata atcgcaaagc gctgcttacc tccaggcata
    63061 gcatctgcaa cggatagccc ttttccgtct cgatcataag ccccttctaa ttgattggca
    63121 gcagttgctc caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc
    63181 ttattttaca attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag
    63241 tatatcggca aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc
    63301 tgctctaatt gtttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc
    63361 ttgtttgact aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg
    63421 aatcaaaatc tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat
    63481 agtcgtgatc tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat
    63541 accttttccc atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac
    63601 tttacccgtt aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt
    63661 cgcatgtttg ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt
    63721 acctacgaat gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga
    63781 ctctacacca tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct
    63841 ggagaaagct tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca
    63901 agcggcaatg aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat
    63961 tccaaataac gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt
    64021 tcgcaatgct acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg
    64081 catcaataca tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatcccca
    64141 atgcatgccg aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat
    64201 tggactaaat ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc
    64261 gattggtcct aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac
    64321 taaaatcatt tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg
    64381 aacccatacg gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat
    64441 cactgggatc ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg
    64501 atacacaagt gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc
    64561 tgctgtaaaa gcaagcatga caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa
    64621 tatttgataa gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa
    64681 tcctttcaat accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat
    64741 aatatcgatc agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc
    64801 aagaccttcc tcatccacga tcgattgata aacatctgct acattactgc caatcactac
    64861 ttgatactga ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg
    64921 cgtatctgct aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa
    64981 acttttgaca ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt
    65041 atccattgtt ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa
    65101 acagtcacaa acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa
    65161 caatccttat gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa
    65221 ataaatctgc tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat
    65281 ccgttcgaca caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc
    65341 atcttgctcg tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt
    65401 aatgatacgc tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat
    65461 gatactcagt atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt
    65521 tccttgttgg ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc
    65581 cacacccgtc ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg
    65641 ataaaatttt tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg
    65701 tgtgatcgca taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac
    65761 ttttagttta tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg
    65821 aatatcagcc atcagctgct ggaattggtt tttagcaaaa gaatctctta aaacaaattc
    65881 tttttggacc gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt
    65941 tcccatacaa acgacttctt ccccgaatcc atttttcgaa atgacgacat tgttattcaa
//
LOCUS       DACDVT010000004        63193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDVT010000004 DACDVT010000000
VERSION     DACDVT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.004"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            537..2009
                     /locus_tag="IWL52_000240"
     CDS             537..2009
                     /locus_tag="IWL52_000240"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9776834.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            2021..2845
                     /gene="nadE"
                     /locus_tag="IWL52_000241"
     CDS             2021..2845
                     /gene="nadE"
                     /locus_tag="IWL52_000241"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9776835.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(2918..3808)
                     /locus_tag="IWL52_000242"
     CDS             complement(2918..3808)
                     /locus_tag="IWL52_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9776836.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            4006..5523
                     /locus_tag="IWL52_000243"
     CDS             4006..5523
                     /locus_tag="IWL52_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9776837.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5513..6784
                     /locus_tag="IWL52_000244"
     CDS             5513..6784
                     /locus_tag="IWL52_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9776838.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            6959..7855
                     /locus_tag="IWL52_000245"
     CDS             6959..7855
                     /locus_tag="IWL52_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9776839.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            7869..8732
                     /locus_tag="IWL52_000246"
     CDS             7869..8732
                     /locus_tag="IWL52_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9776840.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            8738..9301
                     /locus_tag="IWL52_000247"
     CDS             8738..9301
                     /locus_tag="IWL52_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9776841.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            9311..10834
                     /locus_tag="IWL52_000248"
     CDS             9311..10834
                     /locus_tag="IWL52_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9776842.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            10846..11805
                     /locus_tag="IWL52_000249"
     CDS             10846..11805
                     /locus_tag="IWL52_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9776843.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            11891..12970
                     /locus_tag="IWL52_000250"
     CDS             11891..12970
                     /locus_tag="IWL52_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9776844.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(13222..14622)
                     /locus_tag="IWL52_000251"
     CDS             complement(13222..14622)
                     /locus_tag="IWL52_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9776845.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            14840..16501
                     /locus_tag="IWL52_000252"
     CDS             14840..16501
                     /locus_tag="IWL52_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9776846.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16458..17156
                     /locus_tag="IWL52_000253"
     CDS             16458..17156
                     /locus_tag="IWL52_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9776847.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            17172..18596
                     /gene="araA"
                     /locus_tag="IWL52_000254"
     CDS             17172..18596
                     /gene="araA"
                     /locus_tag="IWL52_000254"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9776848.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            18903..20063
                     /locus_tag="IWL52_000255"
     CDS             18903..20063
                     /locus_tag="IWL52_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9776849.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            20181..22040
                     /locus_tag="IWL52_000256"
     CDS             20181..22040
                     /locus_tag="IWL52_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9776850.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            22150..22584
                     /locus_tag="IWL52_000257"
     CDS             22150..22584
                     /locus_tag="IWL52_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776851.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(23023..24045)
                     /locus_tag="IWL52_000258"
     CDS             complement(23023..24045)
                     /locus_tag="IWL52_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9776852.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            24173..24526
                     /locus_tag="IWL52_000259"
     CDS             24173..24526
                     /locus_tag="IWL52_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9776853.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            24538..25704
                     /locus_tag="IWL52_000260"
     CDS             24538..25704
                     /locus_tag="IWL52_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9776854.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(25796..26416)
                     /locus_tag="IWL52_000261"
     CDS             complement(25796..26416)
                     /locus_tag="IWL52_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9776855.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(26608..26904)
                     /locus_tag="IWL52_000262"
     CDS             complement(26608..26904)
                     /locus_tag="IWL52_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9776856.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            27108..27758
                     /locus_tag="IWL52_000263"
     CDS             27108..27758
                     /locus_tag="IWL52_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9776857.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            27774..29501
                     /locus_tag="IWL52_000264"
     CDS             27774..29501
                     /locus_tag="IWL52_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776858.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            29498..31390
                     /locus_tag="IWL52_000265"
     CDS             29498..31390
                     /locus_tag="IWL52_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776859.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            32094..32963
                     /locus_tag="IWL52_000266"
     CDS             32094..32963
                     /locus_tag="IWL52_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9776860.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            33172..34197
                     /locus_tag="IWL52_000267"
     CDS             33172..34197
                     /locus_tag="IWL52_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9776861.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            34236..35186
                     /gene="manA"
                     /locus_tag="IWL52_000268"
     CDS             34236..35186
                     /gene="manA"
                     /locus_tag="IWL52_000268"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9776862.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            35280..36983
                     /locus_tag="IWL52_000269"
     CDS             35280..36983
                     /locus_tag="IWL52_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9776863.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            36985..39018
                     /locus_tag="IWL52_000270"
     CDS             36985..39018
                     /locus_tag="IWL52_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9776864.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            39029..39469
                     /locus_tag="IWL52_000271"
     CDS             39029..39469
                     /locus_tag="IWL52_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9776865.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(39669..40796)
                     /locus_tag="IWL52_000272"
     CDS             complement(39669..40796)
                     /locus_tag="IWL52_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9776866.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(40799..41476)
                     /locus_tag="IWL52_000273"
     CDS             complement(40799..41476)
                     /locus_tag="IWL52_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776867.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            41740..43506
                     /locus_tag="IWL52_000274"
     CDS             41740..43506
                     /locus_tag="IWL52_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9776868.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            43521..43958
                     /locus_tag="IWL52_000275"
     CDS             43521..43958
                     /locus_tag="IWL52_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9776869.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            43971..45122
                     /locus_tag="IWL52_000276"
     CDS             43971..45122
                     /locus_tag="IWL52_000276"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9776870.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(45184..45882)
                     /locus_tag="IWL52_000277"
     CDS             complement(45184..45882)
                     /locus_tag="IWL52_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9776871.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(45875..47227)
                     /locus_tag="IWL52_000278"
     CDS             complement(45875..47227)
                     /locus_tag="IWL52_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9776872.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(47450..48850)
                     /locus_tag="IWL52_000279"
     CDS             complement(47450..48850)
                     /locus_tag="IWL52_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9776873.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            49011..49748
                     /locus_tag="IWL52_000280"
     CDS             49011..49748
                     /locus_tag="IWL52_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9776874.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            49873..50352
                     /locus_tag="IWL52_000281"
     CDS             49873..50352
                     /locus_tag="IWL52_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9776875.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            50349..51116
                     /locus_tag="IWL52_000282"
     CDS             50349..51116
                     /locus_tag="IWL52_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776876.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            51346..51915
                     /gene="lepB"
                     /locus_tag="IWL52_000283"
     CDS             51346..51915
                     /gene="lepB"
                     /locus_tag="IWL52_000283"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9776877.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(52019..52621)
                     /locus_tag="IWL52_000284"
     CDS             complement(52019..52621)
                     /locus_tag="IWL52_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9776878.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            52727..53196
                     /locus_tag="IWL52_000285"
                     /pseudo
     CDS             52727..53196
                     /locus_tag="IWL52_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            53302..53886
                     /locus_tag="IWL52_000286"
     CDS             53302..53886
                     /locus_tag="IWL52_000286"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9776879.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            53889..54962
                     /gene="prfA"
                     /locus_tag="IWL52_000287"
     CDS             53889..54962
                     /gene="prfA"
                     /locus_tag="IWL52_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9776880.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            54955..55794
                     /gene="prmC"
                     /locus_tag="IWL52_000288"
     CDS             54955..55794
                     /gene="prmC"
                     /locus_tag="IWL52_000288"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9776881.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            55787..55966
                     /locus_tag="IWL52_000289"
     CDS             55787..55966
                     /locus_tag="IWL52_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776882.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            55988..57013
                     /locus_tag="IWL52_000290"
     CDS             55988..57013
                     /locus_tag="IWL52_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9776883.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            57188..58432
                     /locus_tag="IWL52_000291"
     CDS             57188..58432
                     /locus_tag="IWL52_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9776884.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            58585..59532
                     /locus_tag="IWL52_000292"
     CDS             58585..59532
                     /locus_tag="IWL52_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9776885.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            59666..60295
                     /gene="upp"
                     /locus_tag="IWL52_000293"
     CDS             59666..60295
                     /gene="upp"
                     /locus_tag="IWL52_000293"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9776886.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(60434..60676)
                     /locus_tag="IWL52_000294"
     CDS             complement(60434..60676)
                     /locus_tag="IWL52_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776887.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            60797..60869
                     /locus_tag="IWL52_000295"
     tRNA            60797..60869
                     /locus_tag="IWL52_000295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60830..60832,aa:Thr,seq:cgt)
     gene            complement(60988..61626)
                     /locus_tag="IWL52_000296"
     CDS             complement(60988..61626)
                     /locus_tag="IWL52_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9776888.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(61749..62381)
                     /locus_tag="IWL52_000297"
     CDS             complement(61749..62381)
                     /locus_tag="IWL52_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9776889.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            62732..63031
                     /locus_tag="IWL52_000298"
     CDS             62732..63031
                     /locus_tag="IWL52_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9776890.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        21024 a        10998 c        12977 g        18194 t
ORIGIN      
        1 ttctattgac agctgctttc ttactttgta tctttttaaa aacgtgtgat attttttccg
       61 cgagtttttt caacgcaagg tatatcttgt cataattcgc ctttccaaaa aaattcaata
      121 cataataagt tatatccgtt tctttgggga taagaagcaa aaaaggaaag acgtattatt
      181 gcttcaaaaa cttttgccat tgattatgtt gtagagacat ttggttatat ttgttcaatc
      241 atcggtatct taacggctac gattgtagta ggaaaatcta cttattttat caagtaagaa
      301 aaatagaaaa cgctaatttc ttcgtgtctc ggatacattg aataaaataa gtattctttc
      361 tcgtttaggt taaataccaa taagaaagga tagttttagc agatggagtt catttttagc
      421 aagaaatatc cgtaaaacat gtgctttttc agaaaaaatg gaagatttct tttcctatta
      481 attttttttg ttataatggg taggaaatga aaacttgcca agaggagtaa tacaagatga
      541 ataatactta cccagacgac agtttgacgc tgcatacgga tctatatcaa atcaatatga
      601 tgcagacata ctgggaatta ggtcgagctg atttacatgc tgtttttgaa tgttactttc
      661 gagaaatgcc ttttaaccac ggatacgcgg tttttgcagg attggaacgg ttagtcaatt
      721 atttagagaa tctgacgttc aatgattccg atattgctta cttgcgtgga ttagaagtct
      781 atcctgaagg ttttttggag tatttacaaa attttgaatt taaagcaaca gttcgttctg
      841 ctcgtgaagg agaactagtt tttgcaaatg aaccattaat ccaagtggaa gggcctttag
      901 cgcactgcca attagtagaa acagcactgt tgaatatggt caatttccaa acgttgatcg
      961 cgacaaaagc agctcgtatc aaatctgtca tcggagaaga tccactattg gaatttggaa
     1021 ctagacgggc acaagaactg gatgcagcag tctggggaac tagagcggca tatatcggcg
     1081 gagcagatgc aacaagcaat gtacgtgctg ggaagatatt tgggatacca gctagcggga
     1141 cccatgctca ttcgttagtc caatcttatg gaaatgatta tgaagcattt atggcctacg
     1201 ctaaaaccca taaagactgc gtgttcttag tcgacactta tgatacgttg aaatcaggtg
     1261 tgccaagtgc gattcgcgtg gcgaaagaat taggtgacaa aatcaacttc caaggcgtac
     1321 gcatcgacag tggagatatg gcctatatat ctaagcgtgt tcgggaacaa ttagatgcag
     1381 ccggatttac ggaagcaaaa atctatgctt cgaatgactt ggatgaagca acgattttga
     1441 atttaaagat gcaaaaagct aaaattgatg tatggggtgt tgggacaaaa ttgatcacag
     1501 cctatgatca gccagccctt ggtgcagtat tcaagctagt atcgatcgaa gatgatgaag
     1561 gaaaaatgat ggatacgatc aagttatcaa gcaatgcaga aaaagtaact actccaggga
     1621 aaaaacaagt gtggcgcatc actcgaaatt ttgatgggaa atctgaagga gactacgtga
     1681 cattatggga tgaagaccct cgagaagaag aagctatctt catgttccat ccagtgcata
     1741 cgtttatcaa taaaactgtt cgagacttta cagctcgccc agttttacaa gatatcttta
     1801 tcgaaggaaa aagagtttac gaattacctg cactaaacga aatcaaagaa tatacaaaag
     1861 aaaatctaga ttcactatgg gaagagtata aacgagactt gaatccgcaa aaatacccag
     1921 tcgatctgtc tactgaatgc tggaaccata aaatggctat catggaacga atgaaaaaaa
     1981 gcgtagcaga acttcataca gaagcatagg aggagaataa atgagcctgc agacagaaat
     2041 tatcaatgaa ttaggtgtca aaccaacaat tgatccaaaa gaagaaatac gtaaaagtat
     2101 tgactttatg aaagcatatt tgaaaaaata tccgtttctt aaaacttttg tactaggtat
     2161 cagcggcggc caagattcta ctttagcagg ccgtttggcg caactaacga tggaagaaat
     2221 gcgtgaagaa acaaaagatg aaaactatca atttatcgct gttcgtcttc cttatggtga
     2281 acaagcagat gaagaagatg caaaagctgc tcttgacttt atccagcccg atgttagtct
     2341 gcgagtaaat atcaaaccag ctgttgatgc acaagtacaa gtattaagcg aagcaggtgt
     2401 gggaatcagc gattttaaca aaggaaatat caaagcacgt caacgtatga ttacgcaata
     2461 tgccgtagct ggagaaagag caggagccgt attagggaca gatcatgctg cagaaaacat
     2521 tactggattc ttcacgaaat ttggtgatgg tggagccgac attttgccat tattccgttt
     2581 ggacaaacga caagggaaac aattgcttca agcattgaat gcaccggaaa aattatacac
     2641 aaaaattccg actgcagatc ttgaagatgg gaaaccaatg attgcagatg aagtcgcact
     2701 aggtgtcaca tataatgaaa ttgatgatta tctagaagga aaagaagttc ccgctcaagc
     2761 acaagaaaaa atcgaagcat ggtggaacaa gacacaacat aaacgacatt tgccaatttc
     2821 cgttttggat gatttctgga aataacaata aagcaaaaac agccgtccct tcatagggat
     2881 tggctgtttt tgttttataa aatagaaaag aaaaatatca gctgtagtaa acttttaatt
     2941 tgatatcctg atcgtaatta ccgaatcctt taccaaataa agtacagccc cccacatgtt
     3001 tggcatcgtc tttcacttcg attcgcaaat gccaagtgtc ttgctcttta gggatgtcgt
     3061 tgatcgtgat gtttgacaag ggttctccat tcatattcgt caagtgtttg gtaattcgga
     3121 tcgtcttcag caagccgtat tgattaagat tatctggata ccaatcaggc gtatatttac
     3181 cgcggatatc agcaaaatct ccagggctcg tccatgttcc tagctccaca ccatttaggc
     3241 tgaaagtgat atctgaaggc caattgtcat ttgaaaaagg aaattcagaa gaaatttcca
     3301 cagaaacttc caacatctcc aatgtatcct ctgtatttaa aaaattaggt gcctgatact
     3361 cgacaaatcc gctggtaaac cagagaatgc gggcatccat ccttcgagga tccataaaat
     3421 agcgtggttc atccactttc ccgataaaat catggatcgt agctaaaccg catgttggtt
     3481 caattgcata atttgtatag tgaccaatag gtatcgatgt ttctttcgta tcaaaagcat
     3541 tgaatatttt ttctggaaaa ctgatatcga ttttatcgac acgaaggctc gaaatttttt
     3601 gttgtcctac tttttctgtt ttgatgatgt ttgcttcctc tagctttttc acgtgcatcg
     3661 tcgtgatagc actgctcact ccaagttctt ttgccaaatc tttgacattc atcttttttt
     3721 tggaaagtag ctggatgatc ttgatccttg ttttactagc taatgcttca tataccggta
     3781 aggattcttc gtttatttgt agttgcatat atatcacctc ttaaaaggtt tttcttatat
     3841 attaacatat ttgttaataa aagttatatt aaattatttg ttttaatcat tattaatatt
     3901 ttggttaatt aaaatcgata aaaaataagt aagtttaaca tttatattat tgacaacgct
     3961 ttcaacgagg tgtattattc atgtataaat taaaggaggt cagttatgga agccacaatg
     4021 ttgattagta gtgtcaagcg tgtaggcaaa gttgatccgc ggttgtacag ttcatttatt
     4081 gaacatatgg gacgagcagt atatgaaggg atttatcagc cagatcatcc atcagctaat
     4141 aaaagtggat ttcgccaaga cgtcattcac ttggtaaaag aattgaacat tcccatgatt
     4201 agatatcctg gtggaaattt tgtgtcagga tttcgttggg aagacagtat tgggcccata
     4261 gcaaatagac ctagaaggtt ggatttagct tggcggacaa ttgaaacgaa tgaagttggg
     4321 attcatgaat tttataactg gtgtcagcat gtgggggccg aaatcaatat ggctgtcaat
     4381 ctgggtactc gtggagtaga tgcagctcgt aatttagtcg aatattgtaa ttttcctaaa
     4441 ggaacttatt ggagtgattt aaggcgttcg aatggtcaag agagcccttt tggaattaag
     4501 acatggtgtt tagggaatga aatggacggt ccttggcaga ttggacataa aacagcggaa
     4561 gaatatggac ggttagcaga ggaaacggct aaggcgatga aactcgtaga tgattcaatt
     4621 gaggtggttt tatgcggaag ttcgaatcgg caaatgccta catttggtga ttgggagtta
     4681 acagttcttg atcaagcgta cgatcaaata gattatttgt ctctgcatca atattatggc
     4741 aaccaaaaaa atgatctttc tcattatctt gcacgttcct tagacatgga tcggttcatt
     4801 tctgaagtga cagcgatgtg cgatacagtc aaggcgaaga aacatagtaa gaagcagatc
     4861 aacctgtcct ttgatgaatg gaatatttgg tatcattcag cggaacaaga tgagcaagca
     4921 gcgccttggc agattgctcc tcctttacta gaagataact ataattttga agatgcatta
     4981 ctattaggct gtctattaat cactttattg aaacatgcag atagagtaaa gattgcttgt
     5041 cttgctcaat tggtaaatgt tattgcacca atcaggacag aaacaaacgg agaagcttgg
     5101 agacagacga tctattatcc atttatgcaa gctgctgtgt atggacaagg agaggtgtta
     5161 agcccacaaa ttcgttcagc aaactatgcg acggaagatt ttgaagaagt gccgtattta
     5221 gaaagtatag ccgtaataaa tgacaaagaa cttgtcatat ttgcagttaa ccgtggagaa
     5281 gaagaaatga atctaactgt tcacttgtct gaattatcat tagagggagt gattgatttt
     5341 tctgaaatga gtggatttga tacaaaagca actaatcttc ctaaaagtga acaagtaaaa
     5401 ccaagtcatt caacaaatgt aaaagctgaa gatgggaaaa tcaattgttt attgaaaccg
     5461 ctatcttgga atgtggtcag atgtcgttta tcagatgaag gaggaacaaa aaatgaaagg
     5521 taaaaaatgg ttaattggct ctttgttggt agctgcaaca ggtattttag gagcttgcgg
     5581 tggcggaagt tcagcagaat ctgattctga taaagaagtg actttgactt tttggaatgg
     5641 gtttactgca tctgatggtg aaatattgca agaaatcgtt aatgaattta ataaaacaaa
     5701 tgataaaaac atcaaaattg aaatggatat catgacttgg gccaatttga atgaaaaact
     5761 tcctacagcg atttcttcta aaaaagcacc tgactttatt gcattaaatt atccagattt
     5821 tgcacagtat gtagaaaacg gagcggtaca accattagac gatttctggg aatatgatgg
     5881 agtagataaa tctgatttta aagaaacggc tattgaatta ggtgaagtag aaggtaaaca
     5941 gtattttgtc cccatgcagg tacaaggcat gtatatgtat tggaataatg atttgtttaa
     6001 acaagctgga ttagatactg aacaaccacc tactacttgg gaagaattga cagaaatggc
     6061 accaaaatta actgatagta gtaaaaatgt atcaggtttt gtcttcaata aggatggaac
     6121 tgcaccgtta tataactgga tgttagcaaa tggcggtaaa ttagtgaacg atgattatac
     6181 taagtctgaa tttgcgtctc ctgagaacct taaaacgtta aaagcgatcc aaaaaatgat
     6241 ccatgtagac aagactggtc cagaatcgat ttctggcgcc gaaatggaca atttaatgaa
     6301 tgctggtcaa ctagccattg aaatcaatgg gccatggtta aataacggat tgaaagcaaa
     6361 cgagattgat tataatgtga ctaccttacc acaagggagt gattcagaca aaaaaacagc
     6421 tattttagat ggcgtaggat atgccattcc ggcaagtaca gataataata aaaaagaagc
     6481 tgtctatgag tttttgaaat attggaatac aactgaaatt ggtaaaaaat ggagtatcga
     6541 aaatggtttt cctgcctact tggattcagt agcaaaagat gaagaagtaa aaaatgatcc
     6601 tattgtatct gaattatcga aacaaatgga ctatgcagag ccatatttac caggttttac
     6661 ccaaatctct gcgatcaata acgatatcat caatccactg attgaaaaat tattagcagg
     6721 agaagatcct gagacactca tgaacgatgc agatgatgcc atcaatcaat tactgcaaaa
     6781 atagtacaga actttataca ggatagactg ttggcaagat acagatatct gaatgatcga
     6841 cctagtattt gcagataggt gttattgcaa aaatcacagc gattgttcgg atatcttatt
     6901 cagattcgtt taaaccaaga ttgtatattt tatcaaaatg aaagatgagg tggactatat
     6961 gaacggattc accaaatatt ggaatcgtcc tgatcgtgct gcgtatttgt ttttaactcc
     7021 atctttttta gttttattat tatttactgt cataccgctt ttaggaacat ttgctattag
     7081 ttttacagat ttgaatattt tctttacatc aagaaatttt gtaagttttg aaaatttcac
     7141 acgtatattt agtgatgaac gagcagttaa ttcgttgatt cattctttat atttcaccgt
     7201 actcgaaacg ccaacacaaa ttatcgttgg tttattagca gcagtagcat tatctaaaaa
     7261 taccagattc aatcgttttt gtcgttcgac attttacatt ccagttattt gttcattgac
     7321 gtcaattgcg attatttttt ctatgttgct tgatccgaat gtaggggcga ttccttactt
     7381 gttttcacaa gtcggcttgc cgatccctca attttttcgc gatccaaccc ttgctatgcc
     7441 gacggtagcc atcatgactg tttggaagaa ttttggtgtt actatgacga ttctcttgac
     7501 tgctattcag ggaatctctc cctctttgta tgaatctgct caaatggatg gtgcaacaaa
     7561 tcgacagcag ttttttaaca ttacgctacc tcagatcgtt ccctccttag gtttttgtat
     7621 tttaacgaat ctgattggtt cgatgcaagt gtttgaccaa gtatatgttg caaccgctgg
     7681 tggcccacaa tttaagacag aaacagctgt tcaatatatt tacagtcgag gatttacagc
     7741 accttatgaa ttgggctatg catcagcact gtcttcggtt ttgtttatca ttgttgcaac
     7801 tatagctatc tccactaatg tgtacatgtc acggaaagaa aaacaaatga aataggaggg
     7861 aaacctgtat ggcaccagaa agctcgatta cagttactgt gaatagtcga aaagcagtta
     7921 gtggtaaaaa aatcattcga acgattagta tgctagttat attgttggtt attgcgtttc
     7981 cttttttgtg gctgattatt tcttctttta aacacgaaaa agacattatc tcatttccgc
     8041 ctagaatatt tgcagatagc tataccctag ataactatat taaggtttgg acaacgatcc
     8101 ctttattaga ttacatcaaa aatacagtga tttttgctgg agggacagtg atcacttctg
     8161 tattctttga ttctcttgcg gggtatgcat ttgctcgtat gcgtttcaaa ggaaaatcag
     8221 ttttgttcta tttcgttttg ctgacaatga tgatcccatt ccaagtcttc atgattcctc
     8281 tatttattga agtcaatttg cttggcatgc ttgatactta tgcaggtttg atcattcctc
     8341 gtatgacaac tgcatttggt atcttcatga tgcgttcctt ttttattact ttaccagatt
     8401 ctttggaaga ggcagcacgc attgatggat taagtgagtt taatattttt ttgaaaatca
     8461 tgttgccatt gagcaaacct actcttttat cattagggat tttcacgctg atgaatagtt
     8521 ggaatgactt gctttatcca ttgatattga caagtagttc aaaaatgaga actttaccag
     8581 cagggttagc tttatttaca gggcagaaca tttcttttta tggacctgta atggctggaa
     8641 cggttatttc aatgctgcca ttactagtgg tttatatttt tgcacagaaa tattttgttc
     8701 aaggaacagc tatgtctgga atgaaagagt gaggaagatg aaaatagttg agactaaact
     8761 ttatggacag ttgattgtgg tttctgatta catcttatta ggtattctct gggtaatcgt
     8821 gtgtttgcct gttattacta tcgtgcctgc tacatgtgct gttttttatg taatggcgca
     8881 atggaaaggt gagggatcag gaaatatcat ttattttttc tttcaaggaa taaagcaaca
     8941 ttttggtaag aacttgttcc ttagtctgtt aacgatcatt atttattttc ttgttaatca
     9001 attattagaa gaacagacaa tcgtactttc aattagtgga tatgtagttt tacttattta
     9061 ttccatgttt cttttatcct ggatatatgt gatttctgaa aacaaagata tcacattttt
     9121 acaaattttt gaacaaagtg catggaaagt attttttgat tttttgagaa atattctttg
     9181 ttgcgtattg ttattaggat ttgtactgct catatttctt ttccctcctt ttatttttat
     9241 ctttgctggt ggcgtatgga aacttgtatc tattatcttg actataggaa atgaaaggta
     9301 gggaaagctg ttggacgaag aagtgaaagt aaaaatcaga gaggatttta cggtaggaga
     9361 gatcagttct cggctgtttg gctcatttgt tgagcatatg ggaagtgtcg tctatacagg
     9421 gatttatgaa ccgggccatg aaacagctga tggtgacggg tttagacaag atgtcctctc
     9481 ttatgtaaag gaattagaat taggtgtcat tcgctatcct ggaggaaact ttacatctgg
     9541 ttatgattgg gtggatactg taggtaggaa agaacaacga ccaaaaaaaa ttgatttagc
     9601 atggcgaggt atagaaccaa attcttttgg actccacgaa ttttttaaat ggatcagtca
     9661 tgtacaagcg aaaccgatat tcacggtgaa tttaggaaca aaaggaattg atgaagcaaa
     9721 aaatttagta gaatattgta attttccaaa aggaaccttc tggagtgatt taagaataaa
     9781 aaatgggcaa aaaaagcctt ttggcgttaa attatggtgt ttagggaatg agctagacgg
     9841 tccatggcaa gtgggggcta agagtgctta tgaatatggt aggttagcaa atgaagcctc
     9901 aaaagtcatg aagttagtgg atgattcaat tgaaacaatc ttagttggaa gctctacgcc
     9961 acgtttacca tcgtaccctg aatgggatcg tatcgcatta gaagaagctt atgagaatgt
    10021 agattattta gctttgcata actatattga taagtatgat gaagaagact tgactaaacc
    10081 tcctaaaaat gagcgtgatg atacagcgac ataccttgca aaagcttatc gattcgatcg
    10141 tcaaattgaa gaaatcgttg ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa
    10201 aacaatgtat ctagcgtttg atgaatggaa tgtacatgcc caaccggaaa aaagacatag
    10261 ggattttgaa gtcggtagtc ccatcgattg gtgttacttt actatggaag atacattatt
    10321 atttggttca ttagggctgg ctattttacg acacgcagat cgcgtaaaga ttagttgtca
    10381 atcattgtta gtaaacacaa tacctttaat actgacagat gctaaaggac aagcatggag
    10441 gaatccaact tattatgtga tgcaacactt atcgaaatat gctaaaggtc aagtattgaa
    10501 acaacaaatc atttgccctg tttatgattg tgaaaagata aaaaatgttc cttatgtgga
    10561 cagtgtgatc gtagataacg gcaatcagct gactattttc ttgatcaatc gcacaaatga
    10621 acaacaaaag ataacgattg aacatgattt caatttgcta gatcaaggag agcactacac
    10681 attaaccagt agcgatctgt tggacaaaaa tacacgtgaa gaaccagaaa aaataaaacc
    10741 tgaaataaac tatttttcaa tagaaaaaca tgacgaatta aatgtaggga tagaaaaata
    10801 ctcttggaat gtaattagaa taaagaaaat gtgaggaaat ttgttatgat gaagacactt
    10861 tataataatc ctatcgttcg gcaacgggca gatccttggg tatataaaca tacagatggt
    10921 tattattatt ttacaggatc ggttccaggc taccaagtga ttgaattaag aagggcaaag
    10981 tcactgaatg aattagaaca tgctgcaagc ctgattgtat ggcaggctca tgaagaagga
    11041 ccgatgagtg aattgatctg ggcacctgaa gtccattata tcaatggaaa atggtatatt
    11101 tactttgctg caagcaatca taaaactata cgtgatgaat ctcatcatca ccgcatgttc
    11161 gtacttgaaa atcagcatat agatccaatg aatacagatt ggatagaaaa gggacaaata
    11221 aaaacgaaaa gggaaacttt cagtttagac ggaacaactt tctcttctca aggaaagcta
    11281 tattatgtgt gggcccaaca agatcctgaa attactggga attctaatct ttatctatcc
    11341 gaaatggata atccttggac gttaaagggg aagcagatcc tcctttctat acctgagtat
    11401 gaatgggaaa aacggggttt ttcagtaaat gaaggtccag cagtccttac tcgcaatggg
    11461 aaaatattca ttacgtattc tggtagtgca acagatgaaa actatgccat gggcctgctg
    11521 tgggctgatg agcacaagaa tcttttatta aaagaatcat ggcataaatt atcggagcca
    11581 gtattcgttt cttctgaaaa aaataagcaa tttggtcctg gtcacaactc gtttacggtt
    11641 tcagaagacg ggtgctttga tattttgatc tatcatgctc gaccagagaa gaaccaaaaa
    11701 ggggatccgt tagacaatcc taatcggcac gcgaacgcgc aatgctttac gtggaatgag
    11761 caaggatttc cagaatttgg tgaacctgtt ccgtatgcct tatgagcaca agaaaccttg
    11821 atttttgctt gaataaacct tgcagactcg ttatgatata tgtacgtaca aataaaaagg
    11881 ggcaaggcaa atggcaaaat atcaagatat tgcagaagag ataaaacaaa agatccttcg
    11941 tgaggaatac gaagtaaata caaccattcc ctcagaattc aagctgcaag aaatatatga
    12001 cgtcagcaga catacgattc ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg
    12061 taaagaaaaa gggtcgggca catatgtaga tgacagctat aagaaagaaa aaacaccatt
    12121 gaacagaaca aaaacgatcg gtgtcatcac cacatatctc tctgattata tttttccgtc
    12181 gattatccga ggaatcgaaa atgaattacg agaatctggg tattcattgc ttttagccag
    12241 tacgaataac gatcacgaat tggagaaaga gtgtttgaag aaaatgattg cttatggggt
    12301 agatggtttg atcgttgagc ctacgaaaag taatcagtat aacccaaact tagcacttta
    12361 tgttgctttg agagaacaag gcattccgat ggtgatgatc aatgctgctt atgaagagtt
    12421 aaatgcctct tatatttgtt tagatgatgt gaaagtcggt tttatggcag cagaatattt
    12481 gataaaaaaa ggacatcagc gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa
    12541 atacaggatg aaaggtttta tcagcgcgtg tgaacagtat aatgtgcgtt ttttgacgga
    12601 agatatcatt actttcacca cggaaacacg agaaacaatc gctgaaaaaa cagtacaacg
    12661 gttgaaagaa gaacaagctt tagatggcat catttgctat aacgatcaaa tagctagttt
    12721 aatgctagat aagttgacag aaaatggcta tcatattcca gatgacctat cgatcgtagg
    12781 aacagacgat tcttctttaa gccatttagg gaataaaaaa ttgacaacat tgacgcatcc
    12841 aaaagaaaag ttgggacaag atgccgcacg gtggatcgtc acaacgattg aaaaaggaca
    12901 ggcacagcca aatattctct atgaacctgt actagttgaa cgggaaacag tgaaagaaaa
    12961 cgaaaaataa gagacgggtt tttaccgcct cttattgctc ctacggttac tcgtcgcaat
    13021 gtttgtgaga gcttttgtca tcgctcaagg ctgaacgttt ctttcaccac ctctccacta
    13081 aacggtgttc tcctaccaac tggtcacaag tcacaatatt catcaaacat gagaagctgt
    13141 tttttgaaat tgttccttgt gactggaaac taagcggttc cttcttattt cttgaagctg
    13201 accacttctg tcacaacctc tttaagaatg atggatagaa atacgtttag gataagtttt
    13261 tttagatgca ctttgttcga tttcctctaa agatttccca cgagtttctg gtacgatctc
    13321 ttgaataaat aaaaccccta aaaagcagat gatcccaaaa atgccgaaaa cgatttcttg
    13381 agacatagaa gcagtcatca caggaaaaag caagccaact aggaaagaac caatccaatt
    13441 aaaagaagag gctaggccag atgctcgccc gcggacagct agtgggaata tttcgcctac
    13501 gattacccat gtcagtggag cccatgtgca agaataaaat gcgacataaa tacataaaaa
    13561 caagagaatc aacaacgaat ttgtatgagc atccagtaca gttcctaaaa cagctggtag
    13621 gatgaaagaa agtcccatca cgctaccgcc tattttcaat aaagttcgcc gattgaactt
    13681 gtctgcaata accaaaaaga gcaaagaacc agcaactaaa atgattcctt ggatgatcgg
    13741 ccacatcaat gcatcgctcg ctgcatgccc agtcgctttt tctacgatca gcggaatata
    13801 gtaaaaaata gcattagctc cttgaaattg ctggaatact gctacaccaa taccggcaat
    13861 gactaaggaa cgatattttt ctaaaaacaa agtatgccaa gaagttccgg ttgtcttttc
    13921 ttgtttgaca ttcagctgga tttgttcgaa ttcctgttca gcttcttttg gtgtccgaat
    13981 atagttcaat actctttttg cctcttccaa gcggccagat tgcataagaa aacgcggtga
    14041 ctctggtaga gctaaaacac cgaaaaataa aatcagcgcg gggacagcag cgagccctaa
    14101 catcacgcgc caagccatcg tttcaggcaa cccttttagc aaataatctg caacatagga
    14161 aaggagcatg ccagaagcaa tcatcacttg attgatccct gacaatcttc cccttagtct
    14221 agcaggagcc atttcagaca tataagcagg aactagggca gatgcagcgc caacagctaa
    14281 acctagaaga atccttgctg caatcagaaa ataagaacca ttgtgagggg cagatgcaga
    14341 aaggacagaa ccagccataa aaataagtgc agaaagaaga atcatttttc gtcgtccaag
    14401 cttatcagaa agaagaccag ataaagagcc gccaaggatc gcacctaaca tgaccgaaga
    14461 cgtgatccat cctatcaaag aagctttacc tgccaaaccc caatcatgct gtaaaaaagg
    14521 taaggcgccg gtcataacac caatatcata tccaaataga atgccgccaa atgcgccaaa
    14581 aaaataaata aacgatgagg aaactttttt ttcttttttc ataagtattc tctccttcta
    14641 gaatttcgag aaggcggttg atcagatttt gtaagcaatt tcattttata acttatgtac
    14701 gtacaaaaca agtgtttttt attgtattct tgtaaataac tgcttgacat gtacgtacaa
    14761 aagtatacaa taatcattgt aagcgctact aaaaagacgg tttgatcagg tttgacgaga
    14821 aggagggagt taaatcatta tgaaggaagt aaatgcgcaa agtatgttag atgtcataga
    14881 tggtcgtaca tctttaggga ttgagtttgg atcaactagg atcaaagctg tattgattga
    14941 ttcatctttt actcctattg ccgctggtag ttatgagtgg gaaaacaaac tagaaaatgg
    15001 tatctggacc tattcattag aagatatttg gaaaggactg cgcgtcagtt accgaaaaat
    15061 ggcagcagaa gtattcgaaa tctatggcga gaatcttacg acaatcgggt cgattgggtt
    15121 cagtgcaatg atgcacggtt atctagcatt tgatagcaag gaacagttac tagtaccatt
    15181 tcgtacttgg cgaaatacaa tcactgaaga agcagaacga atattaacgg aaaaatttca
    15241 atacaatatt ccgcagagat ggagtatcgc tcatttgtat caatctattt tgaataaaga
    15301 agaacatgtg aaagaaatta attttttgac aacgcttgca ggttatgtcc attggcagct
    15361 tactggtgag aaagtactag gcgtaggaga tgcatcaggc atgtttccta tcgatccttc
    15421 aacgaaaacc taccatcaag aaatgaaaaa tatttttggt cagttaatcg aaaaagcgca
    15481 cgtagcgatt gacttggatc acattttgcc taaagttcta gtagcaggag aagaagctgg
    15541 caagttaagc gaatcagggg cttatctgat cgaccctacg ggaaatttga gaccaggaat
    15601 cccggtttgt ccgccagaag gggatgcagg aaccggtatg gtcgctacga acagtgtagc
    15661 caaagggaca gggaacattt cggctggtac ttccgccttc gctatgatcg tattagaaaa
    15721 agaactagaa aatgtccatc cggaaatcga catggtcact acaccttcag gagaactagt
    15781 aggcatggtt catacgaata actgctcttc ggatatcaat gcttgggtca gtatttttga
    15841 agaatttacc aattctttgg gtatggagat aagcagagat aaattattca cggtattatt
    15901 caacaaagcg ctagaaggcg atacggattg cgggggatta ctttcttatg gttatttttc
    15961 cggtgaaaac attacaggag taaatgaagg aaggccatta tttgtacgta caccaggtag
    16021 ccgttttgat ttagcgaatt tcatgcggat ccatttagcc agtgcttttg gtgcaatgag
    16081 aatcggaatg gatatattga aatctgagaa tgtccaaatt gatcgtttag tggggcatgg
    16141 ggggattttt aaaacaccag aagttggaca gcggattcta gcatctgcta tggaggcacc
    16201 agttacagtg atggacacag ccggagaagg tggagcgtgg ggaatcgcgt tattagcagc
    16261 ctatatgatg gatcgacatg ggaagtcttt agaaaacttc ttggcagaag acgtgtttgg
    16321 acaagaccaa ggaatcacca tcacaccttc agcagaagaa atagcaggct atcagatatt
    16381 tatgagacgt taccgtgagg ggatcgacat tgaaaaaaca gcgattcatc aattgcaaga
    16441 aaaaggagtg atagaaaatg ctggaacaac tgaaagaaga agtataccaa gcaaatcttg
    16501 acttgcctaa acacggactc gtcaaatata catggggaaa tgtgagcgct tttgatcctg
    16561 aaacacgata tttcgttatc aaacctagcg gagtcagtta tgaagagatg acagcagacg
    16621 acatggtcgt tgtggacttg gacaatcata tcattgaagg taaactcaat ccctcttcgg
    16681 atacgcctac tcatgctgtt ttatatcgtt catttcctca aatcggagga atcgtccata
    16741 ctcactcgac atgggcgacg atctgggcac aagcaggatt ggatgttcct gcgatgggga
    16801 cgacgcatgc agatactttt tatggatcgg taccttgtgc tcgatttttg acacaacagg
    16861 aaatcgacag cgggtacgaa tatgaaacag gaaaagttat catagaaaca ttcaaagaac
    16921 gcaaaattga tcccttagca attccagggg tacttttaca cggacacggt ccatttactt
    16981 gggggaaaga tgcgcaaagt gcggtaatga acgcagtcgt actagatgaa gtatgcaaga
    17041 tgaatttatt taccagacag ctcaattcat tttcagaaga gctgcctcaa cggattttag
    17101 ataaacatta tttacgaaaa cacggaaaaa atgcgtatta cggacaaaac aaataaaagg
    17161 agtggctaat tatgttgaat attggagaaa aagaattttg gtttgttgta ggatcacagc
    17221 atttatatgg ggaagaagca ctgcgagaag taaagaaaca tgcacaagaa atggtcgatg
    17281 aactgaatga aaatggccaa ttgccttatc caatccgcct tcaagaatta gcagttactg
    17341 ctgatacgat cacaaaaatc atgaaagagg tcaattatcg agaagaagta gcaggtgtga
    17401 tcacttggat gcatactttc tcaccagcaa aaatgtggat ccgcgggaca aagcttctac
    17461 aaaaaccatt gcttcattta gctacgcaat ataacgaaag cattccttgg aaaacgatcg
    17521 atatggattt catgaattta aatcaatccg ctcatggtga ccgcgaatat gggttcatca
    17581 atgctcgatt aaataaacaa aataaaatcg tcgttggcta ctggaaacga cctgagatcc
    17641 aaaaagagat tgctgattgg atggatgttg cagtagccta taatgaaagt tttggaataa
    17701 aagtggctcg ttttggtgac aacatgagaa acgtgggtgt cacagaagga gacaaagtgg
    17761 aagcacagat tcaatttggt tggactgttg attattttgg tatcggagat cttgtacaag
    17821 taatcgatcg cgtatcagac gaagaagtcg atcaattatt tgaagagtac aaggaattgt
    17881 ataccttcga ctatggtgat tatgaagaaa agacatggga ggaacatgta aaagtacaag
    17941 ctcagcagga aatcggtatt cgccgtttct tagaagaagg aggctataac gcttttacga
    18001 ctaattttga agacctgtat ggaatgaaac aactacctgg actagctgta caacgtttga
    18061 tggcagaagg atacggtttt gctggggaag gcgactggaa aactgcagcg attgatcggt
    18121 tgctgaaaat catggctcga gggaaagata caggattcat ggaagattat acttacgagc
    18181 tagctagtgg ccaagaagca atcttggaat cccatatgat ggaagtagat ccgacgcttg
    18241 ctgcgacgaa accaagaatc gttgtttctc ctttatcaat gggagataga gaagatccgg
    18301 cgcgtcttgt atttgatggg aaagcaggag aaggggtagt cgtatcgatg gcagatttcg
    18361 gtacgcatta taaactgctg atcaatgaag tagaagcctt tgaaccaacg actgaagcac
    18421 ctaatttacc tgttgcacgt gtgttatgga aaactaaacc gaatttccat gaaggtgtcc
    18481 attcatggat tcaggcaggt ggcggtcatc ataccgttgt ctcgctaaat ctaacaacag
    18541 atcaaattga aacgtgggca aaattagttg agttggaaac agtggttatt agataaaaag
    18601 aggttgtgaa aaagccactt agttttaaga aataagaaga gattgacgaa atttgctttt
    18661 caactttttt gaaatttagt cttattatca atgtcagcta acggaacacc atttatcaag
    18721 atatctcacc ctcaatattt tgctctcgaa aataaggctt atggcagtaa cttgtcatag
    18781 ccttattttt atcactcagc ctactctgct ctaactgctc tttttgcttc taggaagtca
    18841 aatggtaaac agttgtctaa ttgcagaaat ttcgagatac ttgttagtaa ggagggggtt
    18901 ttatgtatca cgttcatttg attgatgcta ttccagaaca agagatagaa aagttacaag
    18961 gggaatattt caaaataata gatgatttgc cagccgaagg aatagttatt cgtagtacga
    19021 aagtgaaaga tgaatggctt acaccagatt tgctagcaat ttcaagagct ggtgttgggg
    19081 tgaacacgat caacgttgaa aaagcttctg aaaacgggac gatcgtcatg aatacaccag
    19141 gtgtaaatgc taatgcagta aaagagcttg ttttgtgctg tttgctctta tcttcccgtc
    19201 cgataatcga agcaagtcgt atggtacaaa cactaacagg tcctaatatt ttagaacagg
    19261 cagaaaacaa aagaagtgct tatgtgggac gagaattgca aggcaaaacc atcggtcttc
    19321 ttggtcttgg agcgataggc acaaaagtag cgctaagttg ctatagctta gggatggatg
    19381 tcttgggtta ttctatccgc gatgcccagt tggattatgt tcggcaagca gatttagaaa
    19441 cagttctttc tacctcggat tacatcgtag tcatgctgcc gttgacagaa gatacaaaag
    19501 gattgatcga tcaggcgaat atagaaaaaa tgaaaaaaga tgctgtactt atcaatgtag
    19561 gaagaagtga aattgttgat aaatatgctg ttatgcaagc attggaaaaa caacatctgg
    19621 ctaaatactt gactgatttt ccagaagaag agttcttaga aaatgaccgt atacttatgt
    19681 tgcctcatct agggggatcg actcaggaag ctttggcaga cagcggacgt ttggcagttg
    19741 aagccttaaa agattatctt ttgttcggta cggttagaga agcggtcaat tatccatccg
    19801 cacgaatgtt tttccaagcg ccgtttcgat tcactatatt ttatcaaaag aaaaccaata
    19861 tattagcaga gatattttct ctgctgaatg agaacgagct agcgatcgca gatatatcgc
    19921 gtaaccataa gaatgagcat gtctacatat tgattgacat agacagtact gattttgaac
    19981 agctcaatca agtgaaacaa cagctagaaa aaatttctgg agtacgaaag gtccgcttat
    20041 taaagaaacc agaaaggcca taaagaaaga gaatatttgg atttcattaa ttcttctttc
    20101 cgttctttac tttcgaattt cgtacaacta aaataagaga agcaacattc ttttattgat
    20161 agaaatgaag gagcaagcat ttgaaaagat atataagcag actttcgacc aaacagaaaa
    20221 agattttaaa aggaacagca gcaatcatca gtatcttttt tcttgttgtt tttagtaatt
    20281 tatttttgca gtggtgtcaa aatgatctat ccgtagacct tgcgttgaaa tttgcttttt
    20341 cttggcatac ggaaaaattc ttcttagcct gtctcgtctt gctgattatt cttatttttc
    20401 tgattgcact agcaggatct gtccctttag gttcccttac gtatgtcgta gccatcggtg
    20461 tattaggttt tgcaaattat atgaaaatga gttatcggca agaaccgatt tatccagacg
    20521 atttgaagat gatcactgaa atcggattgc tgaaagatat gaccggcact atgctgttta
    20581 cagtgatctt agctgccgca ggaaccgtga taggattgtt ttgctggtac atgtttcgaa
    20641 gcctgaaaaa aggaaggcga ttccaattga tccgattaac gacactgctt gtggcaattg
    20701 gactgctagg gtatatcagt aatttcaaca atcccgataa tttgttgcga aaagcatata
    20761 ataaaacggc actatggata ccttacagcc agaaaatgaa ctactataac actggtttta
    20821 tcggtgggtt tttgtacaat ctgaaggtag aaccaatgga tgaacctgaa ggatattcga
    20881 aagaaaagat caaagaaatc actgaaaaat accagaagct agccgatgaa aaaaataaaa
    20941 ctgtagagga agagtcgccg aacatcgtgt tcgtgatgag tgaaagtttc tcagatccta
    21001 gtcgattaaa cggcgtagaa gtatctggtg agccattggc agattattat gaagtagctg
    21061 atcaaacata tagtggcaat atgctttctc aaaactatgg cggtggaaca gcaaatatcg
    21121 aatttgaagc attgactggt ttttctatgg cgttatttaa tgcgcaactc accacccctt
    21181 acacgatgct tgtaccaaaa atggatcagc ttccttctat cgtttcagcg ttaaacgcac
    21241 aatcatacca gacgacagcg atccatccat acaatacttc gatgtataaa agagaggatg
    21301 tttatcagac attaggattt gaccagttca tcagcgagcg tacgatgacc tatacggata
    21361 cgatagagaa taatccatat atttctgatg agtcagctta caaagaagta atgacacttc
    21421 tcaaggaaga aaaaacacct cagttcattc atttagtcac gatgcagaca catatgccct
    21481 acgatggaaa gtatgataat ctagactatc ctgcaaaaac ttcagatgga agtgggacac
    21541 ttgacttaga gaattatctg caagatattt cctatagcag cacggcactg aagcagttca
    21601 cggaagaatt gaagaatctt tctagaagga ctttagttgt tttttggggg gatcatcttc
    21661 cagggatcta ttcagatacg atccaagcaa aaaatgacaa acaaacattg catgaaacac
    21721 aatttcttat gttcgacagt aaaggagaat tagaaaagca aacgactcac gatgcaatca
    21781 ccagtccgtt ttattttgca gcgaatctaa tggaacagac gaatcagaca acaaatggct
    21841 tttatcagct gcttttatca ttagaacagg aacttccggc gtttgaacga gaactgtact
    21901 atcaaaacgg acagtggtat aaagaagcgc aattcaatcg ctcgcaacaa gagatttacg
    21961 acgaatatca attgatccaa tatgacattg tcgcagggaa acaatatagt ttagcagatg
    22021 gtttctttga gcacgaataa gaatggcgag ctgtatagaa accattaaat tttttcaagt
    22081 acttgtcatt tgaggataaa aacaagtaga ataaataata acaacaatgg agaaagtagg
    22141 aaaacgaaca tgttaccgaa tattggctat tatgtgcagt ccatcgatca aatcgtcaag
    22201 gaaacagaag caatcggaga aggaatgcat ccgaaatatg aagtgatcag aaacgcaatt
    22261 gatgaaggaa aaacaagtga actggacaaa tcgactcttg aagagatcat cctgttattt
    22321 gaagaaggaa caaaaaaata tcgggcaatg cttatgaaag tcagtacttt gcgtccacca
    22381 gcaaaagcaa tgggcattca taaaaatttt gaaaaagctt atctttcata tgtagcaggc
    22441 tgtgaagaaa tgatccaaag tatcgatctt gaatcagggg tagatgaaga tctattcaat
    22501 acatctgaag caaaacaaga ccaagcgaca gaccaattga cagctgccat ccaaaaaatg
    22561 actaatttgt tgttgaaaaa ataaaagaaa tctttgataa caataaaaac tagagttatc
    22621 taatataaat gtaatattac ctttatgata gagtgatcaa aatcctttat gataatgaca
    22681 tacctataac caacaacaac cttgtactta ctcaaaatcg agtaaatact ccttttccgt
    22741 cgagtctaac catctcggcg gttttttatt tgtagaaaag aacatcagga tagacggaaa
    22801 agtcttgtcc tgatgttctt ttcagatagt atggaaagct gattagtttt ctacattctg
    22861 tacatattgt tcaaagtagt tctgtaaatc ggctttgagt ttctgatatt cctcttcgct
    22921 atactcttta tcttcgattt tcccatcaaa gaaaggaata tttagttctt cccgtagtct
    22981 gtctaatacg tcttgctgat tcatttcatt ctccttttct tactaacgat caaaataaac
    23041 acagacacat tctggtacat agacatcttt tttcagtaat tccagtaatc ctgtttcagg
    23101 atgacggcga tataatgtca gattatcggt gttctgattg gcacacacga caaaattatt
    23161 gcttggatct agtgcgaaat cgcggggaaa atccccttct gttgagatga tttgcttgtt
    23221 ttgaatgtgg gaaccatctt caaaaactgc gaatacagca atcgagttat gtcctcggtt
    23281 agaagcataa aggaacttgc catcatttga aatgcggata gctgcccctc cattttcacc
    23341 gtcgaattct tctggcaagg tcgatatttt ctgtaattct tcaaaagcac ctgtttcttt
    23401 attataagaa agcacgcgga ctgtgctgtc taattcacca aataaataag cgaattgtcc
    23461 atttggatga aagacaagat ggcgagggcc tgttcctggc tctgcagtga aggtagttac
    23521 aagattcaat ttgccgtttt cggacacatc ataagtatac acacgatctg taccaagatc
    23581 acatacgact aatcgttggt caggagtcag atccgtatag tggacatgcg catgatcttg
    23641 gtttttatga ggacctgtcg cttcttgatg gaatattgaa tctgctgctt tcagttcgcc
    23701 atctcctaat atatggtaga catttacttc acctttatga tagttcgccc cgtatagtaa
    23761 ttgacgtgct tcatcaaccg ctacataaca caaaggagcg ccagactctg ttaccgcatt
    23821 caaaaaatga aaatccttat cgtacgcacc ggcaccacct aatccatcca cactagtaac
    23881 tgtgtaaaga ttgccagctt tactttttgc tacataggta gggctatttt cttcaacagc
    23941 taatgaaagg ttggctaatt cttctttttc tgtatcaagg tcgatcgtat agattccttt
    24001 actttcgcga cgcgtatagg ttcctaaaat tatcttttcg atcatatgag ctgattcctc
    24061 ctaattgtaa tgaaatagtt ttcgttttta ttctatcaca taattattcc acacggctaa
    24121 agaatgagaa tgtgttatac tgtttttgaa agcaattaaa ggagagagtg caatgattca
    24181 ggcaaagatt acagaaattg ggacagaagc aataaacaag gaagaaccca tgctgatttt
    24241 gttcgataaa acagcaacag ctgccttacg aaaatattcg gtgattcaag aagtatcaaa
    24301 agacgaacct ttttctttga aagcaggcgg aacaattgct tttgatgatc aggtatatac
    24361 gatcgattat gtcggtccga tggccaatgg gaatttaaag acagtagctc acgtatcatt
    24421 gatttttgat gcttgtccag aagaaggaca aattgcaaat ggaatctatc ttcatccgca
    24481 tagactgcct gctattggtt tgggaagcca aattttttat aactaacagg aggaattatg
    24541 gaaggaaaaa ataagaagaa cagaccggta tcgtggtttt ggcgctggtt tttaaataat
    24601 caagtagtca cagccttact tgttgtattg ttaatattgc tgatcgtctt tttatttaca
    24661 aaagtttcat atttgtttgc accaatctgg caatttttag caatcgtagg tctgcctatc
    24721 attttagcag gaatcttgta ttacttgatg aatccggtag ttgattactt tgaaaagcga
    24781 aaagtaccaa ggctttacag tatcattggg ctgtttgttt tggttgttgc actaatcgca
    24841 tgggggagcg tagtgatcat ccctaagatt caggaacaaa cagtgagctt catcagtaac
    24901 ttccctcaat atgtagatac gattgataat aaattaaccg agattttgcg ggacccgctg
    24961 ttcaaccaat ttcgcgaaca gttggaaaca gcaggtgata agttcatgaa ttcggcaggc
    25021 gatatgatcc aagatatatc taagagtaca gttcagagtc ttggaagttt tgtcggcgcg
    25081 gtagcgacta ttttagtggc tctgctgacg atgccgttta tcttatttta tctattgaaa
    25141 gacggtaaac aattagcacc ttattttgtc aaatttttac cgacacgcat gcaaaaaccg
    25201 acgttgaatg tgctgagtga aatgaatagt caagtttctt cttatataag aggacaacta
    25261 actgtggctt ttgctgtggc aatcatgttt atgattggtt ttgcggtcat tggtttagag
    25321 tatgctgtca cgttggggat tattgcgggt tttttaaacc tgatccctta cttaggatca
    25381 tttttagcga tgattccggc tatcttttta gccattgtag caggtccatt catgatcatc
    25441 aaagtactga tcgtttttgc agtagagcag acaatagaag gacgcttgat ctctccatta
    25501 gtactaggga atcagttagc aatccatcca gtaacgattt tattagttct gctgacttct
    25561 gggaaattgt tcggaatcat cggtgtgatc ttggggattc ctgtttatgc agcagctaaa
    25621 gtagtgatta ctcatgtttt tgaatggtac acaacaatct cttctcttta tgaagaaaat
    25681 ccgttgaaaa agcagaaaga ataacaaaaa gagacagatg ggaaaatatc caaggcacaa
    25741 atgctgcttt gaagattttt tcctctgtct cttttgtatg catttatctc aatttttaaa
    25801 atgatttacc taaagcagca gctttatcta atgcattttg catcaactca tctgcacggt
    25861 cagggtcata atcgattcct tcgatgaata gctgatctac ttgatcgatg cctacaaagt
    25921 ttaaaatacc ttttacatat tgagaggcaa agtcttgtcc gttgtataca ccgccattag
    25981 attggatgtg cagtgctttt ttacctgtca ctaatccttc tggcccgttt tctgtatagc
    26041 ggaacgtttt tccagctaca ttgacggtat cgaaccaagc ttttaatttt gttgggatat
    26101 ttagattcca taaggcgttt gcgataacga ttttatcagc agcaaggaac tgatcagtca
    26161 attcattgaa acgagcaatt ttttgttgtt gactttctga taaggaagtg aattctgcac
    26221 cagcacgtaa tgcttcccaa cctgataata attcttcatc gatttccgga atatcatcgc
    26281 gataaagttc caaaacagtc acttcatcat ctggatgatt ttctttataa cttgttaaaa
    26341 aagacgtcaa tgccttaact gtgcgcgatt cttctgcagt taggggatga cctttgacta
    26401 ctaaaacaga tgccatatgc ttaccttctc cttttattgc tatgattgtt ataactatga
    26461 ctaacaaaat cagtttactc gaaaaagaaa aggctatcaa gtcatattga taaggttgaa
    26521 gaataaataa ctaaaaaaag acagggtata aaagaggctg tgacacgatt ttttgtcaca
    26581 gcctcttttg aatctaacaa gcaatattca agcaacactc gactgaagag cttccatccc
    26641 accaatgata taaacagcac gaaaaccatt agcagtaagt tgatcggcta atgcttctga
    26701 tcgcctgcct gtatagctta gaatataata aagtttcttt ggatctagat tttttaattg
    26761 tcttggcaag atagcagcgg ggatagaaag cgagtggctt aagtggtttt ttgtgtaatc
    26821 taaggtattt cttgtatcta aaacgatgct gtcagcatcg agaggcaatt tattgaagtc
    26881 ttggacacta attgattggg ccatttatct catcctttgt ttagtatgtg gctattttaa
    26941 tgggcaactc tcattaattc aaatggaagg attcctctta tcactttttt aatgtatcgg
    27001 atgataatga aaaaaactat gcaaatgtct tgcaaaacag gtaggaggtg ttataatgac
    27061 atagcgtcat atgacaatgt gtcatcttga aagaaaggag aatccatatg ccgacacaga
    27121 catttttcca tttgcctaaa gaaaaacaaa aaagactaat cgaggctgcg agaatcgagt
    27181 tttctagagt acccttaaaa gaagcttcga ttgctaatat cgtcaaatta gcggatatcc
    27241 ctagaggcag tttctatcag tattttgaag ataaagagga tctttatttt tattactttg
    27301 agacaatgag acgtgacagc tctaaagata tgttacaact aatgaaagaa tccgatggtg
    27361 atctattcaa aggattcgaa tcctattttt ctaaaatgat ttatgagatt ttggaaggcg
    27421 agaatgcttc tttttatcgt aacttgttta tgaacatgga ttaccgtgcc tcaagaaaag
    27481 tcgctcctca tacgagtcgc caaaaaacgc ctcacgatcc acataaagaa cacaaacaag
    27541 atgcaaaaga gttgcttgaa ttgatcgatc ggacgaagct gaaagtaaaa aatgatcatg
    27601 aattggaatt actgatccaa ttagtgatga atactgtatt tacaacaatt gcgcattcgt
    27661 atcgcttatt ttcaacatct gaagattact cagtagagca agcaatcagt gaattcaatt
    27721 tgaaactttc ttggctgaaa aacggagtat ataaatagaa agaagggaac tagatgctaa
    27781 aaatcgtaaa acggatctcc ttgacttcag caattgcagc tgcagttttc atggtgatcc
    27841 aagtactggc agatttatat ttgccgacac ttacctccaa tattattgat aaaggggtag
    27901 cgcaaggaga tgtcgattat atttggcata caggttttgt gatgatcggc ttttctttga
    27961 tcagtatctt agctgctatc gcaaatacat tttttgctac tcgtgaatca cagaaactgg
    28021 gtaagcaatt gcgtacggat gtctataaaa aatcagagag tctgacaaaa gatgcgtttg
    28081 ataaatatgg aacggcctct ttgatcacaa gaacgaccaa tgatgtgact caaatccaaa
    28141 tggttacaca aatgttttta cgtatgatga tcaatgcacc aatcacgttg attggcgcga
    28201 gcatacttgc ttatcaaaaa gatcatcagt tgacaaaaat cttcttagtt gtcatcccag
    28261 taatgattat tttgatcggc ggaatcatgt actttgctgt tccattattc aaaagtatgc
    28321 aaaagaaaac tgaccgttta aacctggtat ttcgagaagg attgacaggg gtcagagtga
    28381 ttcgagcatt cgataagaca cgctttgaag aaaatcgttt tgatttagcg aataaagatt
    28441 atacaaatac ggcaatcaaa gttaatacga tcgtcgcatt gatgatgcca atgatgacat
    28501 tgatcatgag cggtacaaac gtagcaatca cttggtttgg tggacactac atagcagata
    28561 tgactttgga agtcggtaat ctgattgcat ttatgactta tgcgatgcag atcttgatca
    28621 gctttatgat gctttccgct atttttatca tggtgcctcg tgcacaagct tcagctgacc
    28681 gtattaatga agtgttggat gaaaaaatcg gtatccatga ccctgaaaat cctaaaacag
    28741 tttcttttgc tggaaaaaac gcaacattgg ccttcaatca tgtcaattat cgatatcatg
    28801 gagcagaaaa acttgcattg gaagatattg atttccaagc caagtcgggc gagatcgtcg
    28861 caatcatagg ggggactggt tcaggtaaaa cgaccttagt caatttgatc cctcgattat
    28921 atgacatcga atctggttcg atccaaataa atggaacgga cattagtgac atgactcaat
    28981 ataatttgcg agaattaatg ggctttgttc ctcaaaaagc agttctgttc tctggaacga
    29041 tccgagacaa tatgcagtat ggtaagccgg atgcaacaga tgaaatgatt tggaaagcat
    29101 tagaaatcgc acaagctaaa gattttgttt cagagatgga agatggctta gatagccatg
    29161 tggaacaagg aggaggaaac ttctctggtg gacaaagaca gcgtctggca attgcacggg
    29221 cattagtcaa aacagcagat atctatgtgt ttgatgattc cttctcagcg ttggatttca
    29281 aaacggatgc caatcttcgc caagcattga aaacgaatat gaaagaaagt atcaccgttt
    29341 tagttgctca acgagtaagt acagtaatgg atgcagatat gatccttgtg ctagatgaag
    29401 gaaaactggt tggcaaagga acacatgaag aactattagc aacaaacgaa acttatcaag
    29461 aaatcgttca ttcacaacta agagaggagg acttagcatg agtaaaacaa acacacgtcc
    29521 ctctcatgga ccagcaggcg gtccaggaca tggtggacct ggcagaaatc tcggtgtaaa
    29581 aggtgaaaag cctaaaaatt tctggggaac ggtacgccga ttgtttggct atatgtctaa
    29641 acgtatggtt gcgatcatcg ctgtatttgt actagcaata gcagccgtga tcttccaaat
    29701 ccaaacacca aaggtcttgg ggaaagcaac cacagaaata ttcaaaggtg taatggctgg
    29761 cgctcaacag atgcaacaag gccaacaagt aacaaaattt ccgatagact ttgataaaat
    29821 cgcgcagatc attgcgacag taatcgtgat gtatttgatt tctgctgtat tcaacttttt
    29881 acagcaattt attatgacac gtgtttccca acgaacagtt tatgaattac gtcatgattt
    29941 ggaagccaaa atgaatcgtg taccgatttc ctattatgat acacatacaa atggggatat
    30001 catgtctcga gcaatcaatg atatggataa catcgcaagc acgctgcagc aaaatttgac
    30061 tcaatttgta actagtgcag tcacattgat tggggtcatc gtgatgatgc tttcaatcag
    30121 ctggcaattg acgttagtcg cgttattgat gatcccttta agcttgatca tcgtgatgat
    30181 cgtagcgccg aaatcacaag tgtttttcgc agatcagcaa aaaagtttag gtttattgaa
    30241 taaccaagta gaagaaacat atggtggtca taccattgtt aaaacattca atcatactga
    30301 aaaagaccag gaagtatttg aaaaagaaaa tcagaatctg tatgccgctg gttggaaagc
    30361 tcaatttatt tcggcaatca tcatgccgtt gatgaatttc gttaaaaatc taggctatgt
    30421 gttcgtcgct gtactaggcg gagtaaaagt tgcaaatggg aacatgacat taggggatgt
    30481 tcaagcgttc ttgcaatata caacacagtt ctctcaacca atcactcaac ttgcaagttt
    30541 gatgaacact atccaatcaa cagttgcttc agccgaacgg gtatttgaag tgttggatga
    30601 agaagagatg tccaatgaaa aatctggcct tccagaagaa aaagatacac catacaaagt
    30661 tcgttttgaa aatgttcaat tcggctacac accagataac ttgctaatga ctgattttaa
    30721 cttggatgtc aaacctggtg agatggtcgc tattgtaggt ccgactggtg cagggaagac
    30781 gacactgatc aaccttcttg aacggttcta cgatgtttct ggaggtagta ttcgttacga
    30841 tggtgtagat acccgtgatc tttcacgtga tgaattgcgc gcacagtttt caatggtact
    30901 gcaagatact tggctgttta caggaagtat ctatgacaat atcaaatatg ggaacgacga
    30961 tgcaacagac gaacaaatca ttgaggctgc taaagctgct cacgtagacg attttgttcg
    31021 taaattgcca gaaggatata acactgtatt aaacgaggat gcaagcaata tttcgcaagg
    31081 gcagcgtcag ctaatcacaa ttgcgcgggc attcttagca aatccagatg tcctgatcct
    31141 tgatgaagca acatctagtg tagatacacg aacagaaata ctgattcaaa aagcgatgaa
    31201 ccgcctattg gaaaaccgga caagtttcgt tgttgctcac cgcttgtcta caatacgcga
    31261 tgcagacaac attattgtga tgaatcatgg ttcgatcgta gaaacaggaa accacgatac
    31321 attgatggaa aaagacggat tctatgcaga tctttataac agtcagtttt ctgaagaaga
    31381 agcttcatag aatagtacaa atttattatt ttttttaggg gctgtgacaa aagtcgtgtc
    31441 acagtccctt tttccgaata aacggtgttc aatcagctgt ccttcggtat tagctcaaaa
    31501 aacggcaaaa tatgagaagc tatttttccg ttttgtatct ctaatactca ggtcaaaccg
    31561 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    31621 caatagtcaa caaacctgag aagctgtttt ttgaaattgc tccttgtgac tcggagttaa
    31681 acggcaggct cacaacctct tggtaaacgg tgttcaaaag ccagtttctc ggtattaagc
    31741 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact
    31801 aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    31861 aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
    31921 tgcagaacag ctttttcaca acctcttttt ataagagttt ttactcatta atttacaaaa
    31981 aacaagcaaa atgcgaattt tctcacattt tttttaacaa aatcaaagtt attgcttgaa
    32041 agaagaagaa tccatcgtta aaataaagct tgacgtagag gggagcaaga aatatgaaag
    32101 attttcaatt gggggctgcc acctatttaa aaagtgtagc cattcgggtg aaagaccgag
    32161 ataaaatgat tgatttttac aagaacgttg ttggctttga tttgaaaaga gaagaaaacg
    32221 aattagccat tttaggaaca gaagaagctg gaagtgaaat gttgctgctg gaagaaagtc
    32281 ctagagcgaa tgatcacatg ggggtgatca aaaagctgaa ccgtttttca ttgattatcc
    32341 ctagcacaga agaaatggca gatatccttt gtcgtatccg taaaatgaat tatccaatca
    32401 aagaagcttt agaagatcat ggacgtatgg ggattttgct cgtagaccca gaagagaatg
    32461 aattggaaat ttaccatgaa gagaataaga acgaccaaag agaaattcca gaaacaatgg
    32521 atcaagaagc attattaaga aaatctgaag aaaaattcag taaactatca acaggttctt
    32581 attttgaaaa aatccattta aatgttacta acttaacaaa agaacgccaa tttcttaatc
    32641 atgtacttgg tttgaaggta cataatgaag aagagggcat ccatgtattg aatgaaggag
    32701 atttccatgt tggtttgaca gaagctagcg gaggcgcaat tgatttgcct acagatgaag
    32761 tgattggact tgattttctg aaattcatga tcgatactga aagcatggaa ggactgattg
    32821 ctcacttgca gcaactatca gttgatttct tcttagacaa aaagcacaca gtactcacaa
    32881 tctatgatgc tataggaatc gaatggtggt tcgtcagtga gccaaaagaa aagaataaaa
    32941 ctgacattcg atgcgacgct taactttttc tttcttaacg attgtcgcat gtctcgtcat
    33001 tttatgaacg gtcatactat cgcttattct agagatagaa agagaaaata gtataccggg
    33061 ggaaactgcg cggtttttcg cgcagttttt ttgtgtaaaa aaggttgatt cctcatagat
    33121 taagctttaa gcttggactg taagaatctg atagaaaagc aggagaaaaa gatgtatcca
    33181 cacacgaaac agcttatttt gaaaaagatg gaagaaggat tttatccagg tgtagcagtc
    33241 gccttcataa aagaaaatca agtagaaaca tttacagccg gctttgccca agtcgttcct
    33301 gaaaagcagc tgatgacaga agatctgttg tttgatgcag cctcactgac gaaagtcgtt
    33361 gcaaccactt cattagtttt acgtctatta gaagatggaa agatagaact cgatcaaccg
    33421 cttcataaat atcttcctga atttgaaaat cagacaatca ctttgcgtca tttactgaca
    33481 catacttctg atatcaaaac atggatacca aacagagata agctatctca ggaagaattg
    33541 atcgccgctt atttgaagct agaaccaggt gagcaactag gaaaagaagt aaaatataca
    33601 gatgccggta cgattcttct tgggttgatg ctagaggaaa tttaccaaga aactgcagtg
    33661 gaaatctttc aagaacaagt attggaacca ttaggaatga tgaacagctt atttcttcct
    33721 tttccttcca aaaaaatcgt tcccacagaa caactgcctt cgggtgaggt tttgcgtgga
    33781 caaactcatg atccgaaagc aagagttttg gctgaacatg caggtaacgc gggtctcttt
    33841 attgatttga aagacagcat tacttttgtc caaatgtatt tgaacaaggg aaaaatccgt
    33901 aatggtcgtt ttttagaaga agagacgatt gattctttac tgcaagatca aacaccgaaa
    33961 aaaaatggct atcgttcttt aggatgggac ttaaaaaagg atgtagaaga cggtcatgcg
    34021 attctttttc atactggtta tacaggtacg tttttcttga ttgatataaa aacacaagaa
    34081 gcgttgatat ttttgtcaaa tcgcgtacat cctgtcgatc atcgtgcgga gtatctcata
    34141 tgtcgagatg aaattgttga aaactttttg aaagagaaag cggtattacc gaaataatgc
    34201 tataatagct atgaaaaatg agaggagttt gaattatgca ggaacctatg tttttaaaac
    34261 cagtttttca agaaaaaatt tggggaggaa gtcgtttacg ttcagtcttt ggttttgata
    34321 tccctaacga taaaatcgga gaagattggg cgatcagtgc gcatcctcat ggtgtcagtg
    34381 tagtagaaaa tggcgaattc aaagggaaaa gattagatga actatggtca gaacacaagg
    34441 aattgtttgg ccatccttca gaaccagtgt ttccattatt gatcaagatc ttagacgcag
    34501 aagatgatct atctgtacaa gtacatccag atgatgccta tgggatgaaa catgaaggtg
    34561 aattaggaaa aactgaatgc tggtatatca tcgatgctga acctggtgca gaaatcatct
    34621 atggtcatca tgcaaagaca agagaagaac tagctgagat gatcaaagat gggcgctggg
    34681 atgacctttt gaagaaagta ccagttaaaa aaggtgattt cttttatgta ccaagcggaa
    34741 cgatccatgc tattggtaaa ggaatcatga ttttagaaac acagcaaagt tctgacacga
    34801 cttaccgcgt gtatgactac gacagaaaag atgcgcaagg acaaacaaga gagctgcata
    34861 tccaacaatc gatcgatgta acaactgtcc ctgcgaaaac accagaatta caaatcaaag
    34921 aagtccgaaa agggaattca tcgatcgtca cttatttaga aactgagttt ttcaatgtat
    34981 atgagtggga tattaaagga atcacatctt tcaaaaaaca agccccttat acattgatga
    35041 cggtaatcga aggagcagga gaccttgtcg ttgacggaaa aacatatcca ttagaaaaag
    35101 gaacaagctg tattattcca aatggcgttt ctgagtgggc ggtccaaggt gaactagcaa
    35161 tcattgcgtc tgaaccaggc aaatagttcc cagatagctg aaagcagcag acggtttttc
    35221 gtttgctgct ttttctacat aaaatccgtc catcaataaa tagaagtctg gtgaaaaaga
    35281 tggaatacaa atggaaagca aacaacagca gtcgcgtgtc tatgaaacaa ctgagaaaag
    35341 ctggacatca cttaaatgaa atgatcatgc caaatctaag tctgtttatc gcttggggaa
    35401 tcttgtcatt agcaacaaaa ggtgtgtctg gcgatttgca caccacatta gaagaggtat
    35461 gccattggat gatccagctg cttttaccca tcttgatcag ttattcaggc gggaaatcgg
    35521 tagggggaca aaaaggggca gtcgccggtg caattgcttc tttagggttg atcgctcgtt
    35581 ccaatgctcc acaaatcgta ggagcgatga ttatcgggcc gcttgccggc atcgtatatg
    35641 aacaatttgt ttctagattc catgtgaaat tcaaggctgg atatgaaatg cttgtcagca
    35701 acctactagt aggactgact ggtagcgtta tttgtataat tggaatcgtt tttattgaac
    35761 cagttttaaa tagtgttcac ttgttgctcg cctccgttgt gtcttggcta gtcacaatga
    35821 atctgttacc acttgtcagt ctgatcttag aacctctaaa agtactattt ttcaataatg
    35881 caatcaatca tggtgtattg acacctttag gaatcgaatt ctcacaaaca gtcgggtact
    35941 cttccttatt tttgatggag gcaaatcctg gacctggttt agggatctta ctttctatcc
    36001 tttgttttgc agaaaaacag gaaaaagtaa atgcgagtgg agcattgatg atccatgcga
    36061 ttggcgggat tcacgaaatc tattttccat ttgtcttgct gcgaccgtat ctatttttag
    36121 ctgtaatggc tggtggggca agcggcacgt tgatatttca atggttagat acgggattag
    36181 ctgcacctgt atcgcctggg tcactactcg tgatttttgc caatgcgcct tatccacaac
    36241 tagctggggt aagtataggg atagcaatca gtacatttgt gaccttcatt tgtgcagcga
    36301 tactattgaa attaccaaat aaagaaaaag agaacaaaac ggtcaaagaa aggggagaaa
    36361 tcttggaacc atctatcatt cattccgtca tttttgcttg cgactcaggt atgggatcaa
    36421 gtgctatggg agccgccctg ctgaaaaaga aattagcagc agaaaaaatc gatctttctg
    36481 tcggatacag ctctatctat gaattgaata accaagtaaa tcagctggtt atcgtgcaac
    36541 atgaactagc gggtcttgca agagaaaaag tacctcaaag tcaaatctat gtactggatc
    36601 actttttgga aacagatatc ttgctgtcac gaataaaaga agcgatccaa tttccaaagc
    36661 gggaagaaaa aaaagagata gcagatatca cagaacaaaa aacgtcgcct gctaatattg
    36721 tatttttgta taaaaacaac agacgcggct cacaaactat gggcatgacg attttaagtc
    36781 aattagcaaa aaaagcgaaa aaacagtttg ttatctcaaa agaatcaata gaaaaaatca
    36841 aaatccaaga agaaacgatt tatatcgcac aaagagaact agctgtaact gaacagctag
    36901 aagaaaagat ccctcggcta gttctagttg aacattgggt agctacgaat gaatacgaaa
    36961 aactcgtgaa gggagaaata taagatgtat ttatcgccca gagaaaaaca attattagca
    37021 gaatttctca gtagcccgtc acctgtatct atccaaaaaa tgatgaattt attgaaggtc
    37081 agtaaacgga ctatttaccg cgaattagat aatcttgaag tctcattgaa atcggtaaat
    37141 gcttctttga aaaaagcagc tagaggctca tttgtcattg aggcatcaga agaagtatta
    37201 gagcaattaa aagaaaatac agatgggaaa agtaatgact tatccactgc taaacgacaa
    37261 cacgcgattt tagccgagtt gttactgacg acagagccgt tgagtcttgc tcatttcatg
    37321 gaatcatatc gcatcagtaa taccactttt tatgcagata tcaaacagtt ggaagaaagt
    37381 attcgccaac tgcctttgga aatcattcgg aaccagggat acgagatcga aggaccggaa
    37441 aaataccgcc ggcttctgac agcgaatgta ttagaattag aaatcaatga atatgaactt
    37501 tttcatagta tttctttcga tgcatcactg aattattttt ttcgattcgt tgatccgcaa
    37561 catctatctc ttgcacgcaa agtaatcggg gaagaattga tccaaaaaaa gaccaatcta
    37621 agtgatagaa agcttgaaca tttggttttg atgctgacat tgacgatgga gcgtgtgacg
    37681 aaaaatcatt tactgacaaa tgagacatat aatagtctgg caaacaaaga attactacag
    37741 acagctaaac gattgttttc acggatcgct tctgagacaa aacaacttta tccagtaaat
    37801 gagatcgtct tttttgcagg attattaaat gacttcacga actcatttga tcagaatttc
    37861 tttgaagaga cgttcgatac acagcttgct tatttagtaa aacaactgat tcaagaagta
    37921 agtgaagaga cgaatgtcca tttttatgaa gatgaaactt tgtacaaaat gctgttgact
    37981 catttatctg gtgtgttttc aagagccgtt ttacaagaag agacactgac caatccgatt
    38041 ttagaaaaaa tcatggttca atacgaggaa gttgcgaatg caatccgtaa agcagtagag
    38101 cggatttttc aagaaaaaaa attatccgaa gaagagatcg cttatatggt ccttcatttt
    38161 gcgaattcat tggaacgaag tccgaaagaa agcaatatca gtattgcagg attttcgcca
    38221 agtggattag cttctactag catgttggaa ctacgattga aaaggtattt tcccttcatc
    38281 caacaaattc atttctatcg gattgctgat ttaaaaaaaa tcgatttaca agaagaatat
    38341 gatgttgtta tttctacatc tattttacca ggatacggtg gaaaatatat tttagtctcc
    38401 ccgctactgc tagaagatga agtcaagcag ctaaaagaag ctttttcgca tattgagaag
    38461 aaaaaaaggc aaaaaattac gtataacagc aataacgagt ggatgataga agatacttat
    38521 gaggaaacaa tgcatttcat ggatcaaatc aatcaattac tcggtcgttt ttttatccaa
    38581 gaactggata atcctgagac gatttccgaa acgattcgca gcttatttcc ttattttcct
    38641 gaaggactag taacggatga agaggtggtt catcgccggc tgcttaagcg atatgaacaa
    38701 gcacctatcg gtattcctca tacaaatatg gggttattcc atacatccag ctcattgatt
    38761 tctaaaccga tcttttgtat tttcaatttg aaacatccgt tgaccattga agggatggat
    38821 agacagccag tcgatttgtc ccgtatgtta gttatgctgg cgccttcgcc aatccaagaa
    38881 aaagacggga ttcttttagg aaagatcagt ggagcaatca tcatgaatga cttgaacacc
    38941 gagatcttcc gctcaggaaa taaagaaatc gtttatcagt tgttagcgaa aatcctgatt
    39001 gaagaaataa agaaatgaag gtgaaaagat gatacagcta ccagcagaaa aaatattgct
    39061 cgatcaatcc tatcaaaatt ggaaagaagc gttgaccgag atcggccagt ggatgaccca
    39121 acaaggggga atcaaaccgg catatatcca atcaatgatc gaaagacaag aaaaagcgag
    39181 tgtctatatc gggaatttcg tagccttgcc acatgggact gacaaagatg aagaagtagt
    39241 agaagaagga atctatttag tccaagtgcc tgatggtgtg gactttggaa caaaagagga
    39301 accaaaaatc gcgacactct tatttgtagt ggctttaaaa aaagaacatc agttagcggt
    39361 gctccaagat ttagcctttt tctgttcaga catcgaccaa gtaatggcgc tcagtgatgc
    39421 ggaaaccatt gaagaagtcc aaatgatttt tgaacatgga catatataac caatgttcac
    39481 agtaaaaaaa gctgccttgc atcaagtaat aacttcaacc aagataaaaa taagaggagc
    39541 tatttttgtt gggaggttct tattactgcg gatgcaagct tttgaaaact atttattcta
    39601 gaaaaccgtt tatttgaaat aggggacggt gacaaggttt ttgtcaccgc ccctattatt
    39661 tttgtgtttt aatagctctt ttttatatag atagtgccca tacctatgaa taataaaacg
    39721 atactccata taccaagtgt caagatacca ttcatgaaag taacctgttc tgaagctagt
    39781 ggcgagaaaa tatctcctcc tatgatcaca ttttgaaaat caacataaga agttggcaag
    39841 taatgactga ttgattctat ccatccaatc gaagaaaaca agcgatactc tgctaagaaa
    39901 agacaaagaa gtgtacctaa aagattgact agagaatgtt caaataaagc agatagaaga
    39961 aaactgattg tacttaaaaa gagcacccac aacaacagga acagaaagct ctggaaaaga
    40021 tactcataca tcggcaggat tcgtatctcc tgaccaatga tcgttcctac aggataacgg
    40081 aagtctccca aaccattttt gattattact agtatagaga cgatcaaaaa tggcatcacg
    40141 agacttagca tagctgcacc ataataaatg gtaaaacgga tgctatgttt ctttaccata
    40201 ctatccggta atagattgac tagagagatt acctgctttc ttttttcata agagatgatg
    40261 gaagaaagaa acaaggcggt tatacataaa atcaaaaaac tagaaatcat tcctgaaaaa
    40321 atattttcgt agtaatttgc taaagagaga tgcgctggca agtcaaaaat agaatatctt
    40381 tggatctgat gttctttcat ataacggagc aactcgacac gtttttgttg ttcaatgatt
    40441 ggaattcctt gtctttgacc agaaatcagt cgatccagta tatctttttc ataatgataa
    40501 gttgcttcga ccatttgctg gacattccct gtgttttttg catgtaagat tgcttcgatc
    40561 agtttgttgc ttttctctaa actttccaca aattctttct cattctcatc ttctttcaac
    40621 gtttcaatca aacgttgcat ctcttcatag ttatgattca gttcatcgaa ttctgtataa
    40681 gcaagtttat gatcaaaaaa aagtgggaat gcaaagagca cgaccaacaa tagaggaaat
    40741 aaaaagttta ctttgttttt tagtaagaga ttgcgttcaa atgtaagtaa agacatagtt
    40801 acacctcata taatttacga taaatagcta aaatctcttc catatttgag gattctgtga
    40861 cgatttctcc attttttaga aaacatgctt tagaacaaat cgattggata ttcgcgatag
    40921 cgtgggaaga aagaagaatc gtcttcccct ctttctgcca ttgtctgatg aggctattca
    40981 agatatcaat acttcctggg tctaatccgt tcaacggttc atcgaataac atgacagatg
    41041 aatcactgat caagtaagca gcaagcaaga catgttgttt cattcctaga gacatttttc
    41101 caatcggcaa agtaaggtac tcagtcatct tcaatcgttg gaaaacagtt tggcattcta
    41161 ctttgctctg ccaatagtat ttgatcaaag tcaaataatc aacagccgat agatagtcgg
    41221 ataactgatt cgttgattct agaaaaaaga aagaccggcg ataatctgta taagcagtag
    41281 gagagatgtt gtgaagggac aattgatcat aagttgttgg cagcagttgg gcaatgccat
    41341 tcagcaaagt tgttttgcca aatccgttcg gggcaatcaa agcagctact tctcctgatt
    41401 ctactaaaag attcaattgt cgaatcacac gttttttacc acgtttcatg ttcatgtttt
    41461 tgatcagtaa tgtcatagat aggcctccaa tataaaattt aataacataa tagaatggaa
    41521 aacatctaaa ttaaatgcta acaaaagtta ttgtttgcat attataaaaa aattaaaata
    41581 acagttttat ttaaaattga agttttctga taccgaaatc tttttatttt gttgacatac
    41641 tcaaaccttt ggcacaaagt ttcgtgccaa agtacgacta tttctctttt tagtaagcgg
    41701 atacaattag agtatcaaaa ggagagaggg agagacaaaa tggaacggac gattcccaaa
    41761 aacaacattt cgctaaaagc gagggtacaa aaattaggca gtactttatc aagcatggtc
    41821 atgccaaata ttggagcact gattgcctgg ggtgtattaa cggctctatt tatcccagat
    41881 ggttatttac caaatgaggc tttcgctacc atggtaagcc caatgctaac ttatctgatt
    41941 cctttattga tcggttatac tggcggaaaa gtaattgctg gtgaccgcgg atcagttgtt
    42001 ggggcaattg caacgatggg tgtgattgtc ggaacagata ttccaatgat gctaggcgcg
    42061 atgatcatgg gacctcttgg tggatatgcc atcaagaaat ttgatcagct attccaaaaa
    42121 cggatcaaat ccggttttga aatgctagtc aataattttt cagcaggtct tatcggtttt
    42181 ggattagcgt tattaggttt ttctgcgatt ggtccagttg tcgatgcatt gactcaagca
    42241 atggcaaaag gtgtagagat cattttatct gcccatttga ttccattgac aagtattttt
    42301 attgaaccag caaaaatctt atttttgaat aatgcaatca atcatgggat tttgacacca
    42361 ttgggaactg aacaagtagc agaggctgga aaatctattc tattcttgct tgaagcaaat
    42421 cctggtccag gacttggcgt attacttgct tttactttgt ttggcaaagg agcagcaaaa
    42481 tcttcagcac caggagccat gatcattcac tttttaggcg ggatccatga aatctatttt
    42541 ccttacgtta tgatgaaacc gttattattc ttatcagtta taggcggagg aatgtcagga
    42601 agtttcattt tccaattgat ggatgctgga ctaagagcgc cagcttctcc agggtcgatc
    42661 attgcgattc tagcgatgac accgttcaat tcttaccttc ctgttatttt gggcgtgatt
    42721 gttgccgcag ctgtttcgtt tggtatttct gcagtaattt tgaaagcgga tgcgaaagat
    42781 acaaatgaag acttcgaaaa atctgttcaa gagacaaagg cagctaagca agcttccaaa
    42841 ggtgcccaag gtattcctca aggagctaca ttctctggcg tacaaaaaat catttttgct
    42901 tgtgatgcag gaatgggatc aagtgcgatg ggcgcttcga tcttgagaaa aaaagtacag
    42961 gaagcagact tgcctcaaac ggtgaccaat caagcaatca gccaactgac agatgactcg
    43021 aatacattga tcgttactca ggtcgaacta caagaaagag caaaacaaaa agcaccgagc
    43081 gcgcgttttg tctcagtaga aaactttttg aattcacctc gatatgatga aatcattcaa
    43141 gaattaagca gcaagcagaa aacatcagtt tctgaaattg agaatgagcc agcagaagag
    43201 tttctttcag caaacatcaa acaaatcaaa gagattctac ttgtccacga tgatcgttcc
    43261 ggatcagcaa caatggggat gaccgtgctc aaagatatat tagacaagaa ccatttaact
    43321 atcccgttaa gaaaagtcaa tatccaagat ttaaaagatg aacctgattc actgatcgta
    43381 gccaaagaaa gtttagcgga aaaagtacag atggctgcac ctcaatccat tcatttatca
    43441 gtcaacagca tggtcaatcc gcaaaaatac gaatcgattg cacaatattt gaaacagaca
    43501 gcttaaggga ggaaaacagc atgcaaagtt tgccattcga catgatcgag ctcaatcgat
    43561 cttttaaaac caaagaagaa gcgatccgct attgcggtca gatgcttgta gaagcagggt
    43621 gcgttgatga ggaatatatc gatgccatgg tagatagaga caaaatgtta tctgtttata
    43681 tggggaattt tatcgcgatt cctcatggaa cagatgaagc aaaaaaatat gtgaaaaaat
    43741 ccggcatctg tgtcgttcaa gtaccagatg gtgtagattt tggtaccgaa gaagaagaaa
    43801 agattgcgac tgttttgttc ggtattgcag gagtcggaga tgagcatttg caacttgtac
    43861 agcaaatcgc actttattgc agtgatatgg acaatgttgt ccagctagca gacgcgctca
    43921 gcaaagaaga agtaacggaa aatctagcaa ttgcttaagg agagagaaaa atgagagcag
    43981 tacattttgg tgcaggaaat atcggtcgag gatttattgg agaggttttg gcagctaatg
    44041 gatttgagat tactttcgta gatgtgaatc acacgatcat tgatgcttta aatgaaagaa
    44101 aagaatacga aatcgaatta gcagatgaat caaaagaaaa aatcactgta aaaaatgtca
    44161 aaggaatcaa caatcaaaca aatccagaaa aagtaatcca agaaatcgct gaagcagatt
    44221 tagttactac cgccattggc ccgaatatct taccttttat tgctgagttg attgcaaaag
    44281 gtatccaaga aagagaaaag gaaggaagca caacatcttt agatatcatt gcctgtgaaa
    44341 atatgattgg cggttcagca tttctagaaa aagaagtgta taaatatctt ccggaaacat
    44401 catttactga caaatatatc ggctttccaa atgcagcagt cgatcgaatc gtcccattac
    44461 aacaccatga agatccttta ttcgtccaag tggaaccttt caaagaatgg gtcattgacg
    44521 atagccaaag aaaaaataaa aaaatccaat taaaaggggt tttatatgta gatgatctag
    44581 aaccatatat cgaacgcaaa ctatttagtg tcaatactgg tcatgcgact gtcgcttata
    44641 caggtgcctt gttaggttac cagaccattg atgaagcaat gcaagatgcg ttagttgtgg
    44701 cacaattaaa atcagttttg caggaaacag gaagtttgct gattgccaaa tgggggtttg
    44761 atgcagagca gcatcatgcg tatattgaaa aaatcattca tcgcttccaa aacaaaaata
    44821 tctctgatgc gatcacacgt gtcgctcgga caccattacg taagttaggt tatcaagaaa
    44881 gatttacacg tccagtgaga gaactacaag aacataactt gacttgccca catttgacgg
    44941 caacaatggg gattattttt aattactatg atcctgaaga tgaacaaagc cgtcaattgc
    45001 atgaaatgaa gatccatgaa aacttggaac aattgattca agaagtcaca ggaatcaatg
    45061 atccgaaaac aatcgggaat atcaagcaaa acgtgaaccg ttatgccaag caagtcgctt
    45121 aaaataggat acgaaaaagg ggaatgtgac gaaatttttg tcacattccc ctttttcgaa
    45181 taactaggct gtttgtcctt caagataggg cagatccaat tctttaccgt aacgttgttc
    45241 taaagctagg cggatcaaac gagctgccag gttctcattt cttgcaagga taggtccatg
    45301 gaaataagaa ccgaagacat ttcggtaaat gacaccttca gattggtctt caccattatt
    45361 tccttgacct tgtaccactt tccccaaagg tcgttcacct tcacctaaga aagtccgacc
    45421 attgtggttt tcaaatccat aataagtttc attgaattct tcgttatgga taacgatatc
    45481 accgataaac cggttatttt cctgacttaa cgtgtagtga tccagtgcac tgattccttg
    45541 gatcttttct ccattagctc ccatgtagta gtgacctagc aattggtagc caccacaaat
    45601 ggctaacatg acgccatcat tttcgatata gttgatcaaa gaatctttct ttttctggat
    45661 gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg ccaaagaaaa ccaagtcata
    45721 tttatcagca tcaaaaggtt catgtatact aacgatctcc gtatggcaag aaacccccat
    45781 tttttctgct atatatttca acatcaaaag gtttccgtta tcaccatatg tgttcatcaa
    45841 atttccataa aggtgggcga tattgagttc ataattagcc acggttcatt cctcctttga
    45901 taaatccttg agcagctagt tctttacgaa gctgcaatac agcagtgtaa gtagctaaaa
    45961 tatagacatg ctcagttggc aattctttga ttttttcgat tacttctggt aattcttttg
    46021 tttctgtcaa tttattttct ggaatcccgg caacttttag acgtaaagtc atatctgtat
    46081 gacggtctcc tccagaaata acagctggga tatccatatc ggcaaagttt tcaaaattac
    46141 catcccaaat ccagctgaca tcgattccgt ctgcgtaatt ggcatttaat aaagccacta
    46201 aagaaaatgg ataaggagct agaccaatca tatcgattac ttgattgata cctacaggat
    46261 ttttgacaag tactaatgta cattttttcc cattgatatt gatgacttct tgtcggccga
    46321 atactttctc gtcatatcct aaaccttgtt tgattttatc aggatcgaca ttgaagtact
    46381 ctgccactgc agtagctgct aaagcattgt agacattata cataccgcca acttcgattc
    46441 cgtattcatg accatcgatc acgaaatctg ctgacacatt gtccatccgt accatatcag
    46501 ttaatcgata atccaattca ggacgtttga agtcacaatt tggacagtaa tatttcccta
    46561 agtttgcata agtgatcatt ttataatgca agatgtgatg acaatttgga cataagacac
    46621 cgtctgtatt gtaatgagcc atttgttcac gatcttcttc atggtcaaag ccataatatt
    46681 ttctaggatt gactgtttcg atagaattga agattggtga atcaccgtta ctgataatcg
    46741 ttgcatttgg tgactttgcg gcaccgtcta caatcatttt ataggtcgta tagatttctc
    46801 cataccgatc catttgatca cggaaaatat ttgtaaataa gaacagttct ggtttcatat
    46861 attctgtgac tttgctcaaa cttgcttcat ctatttctag gacagcaaac tttttctgtc
    46921 ctttttttgg ttttgcctgc aagaaagtcg atacaattcc ctgtaccata ttcgcaccag
    46981 tagggttggt tagaacttca tcgaattgct ggcgcaaaat attgactgtt aaagcggtag
    47041 tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg gtagtctttt gccaaactat
    47101 ctagtatatg tggatcgact ttcagcgcta gtttacctgg taaacttgat ccgcctttaa
    47161 aaaaagtttt taatgcccat tgcgacgttt ttccaactgc aatcgcaaaa tgactgcgta
    47221 ttcccattat gtaacctcca aaaacgattt gctgacgaat aaattcaact aatcatacca
    47281 tatgttgaaa aaaaggttaa attgcttttc ttattcttaa gaaaaggtta cggctgaaag
    47341 tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa cagcttgaaa caaaatgatt
    47401 gtttcaagct gctgtgattt tattattccc tcttacctat attcccattt taaccgataa
    47461 taattttctc gcttggatat tcatagccta gatctcgtgt ttcttttggc acgaataaca
    47521 tcaatgtata tagcattccg atccgcccaa taaacatcag tgtagcaatc gtgattttgc
    47581 ccacaacaga caagtctccg gtaatcccta aagataaacc agttgttcca aacgcagaag
    47641 ttacttcgat gatgatcgag atcagcgttt gttcttctgt agcagataaa aaaacgatac
    47701 aaaagaaaca cataccaaga gaaagcataa agacaacgac tgattttcgc acatcatctt
    47761 gatcgattct ccgtccaaaa atattgatgt tatcttcact tttgaggaag gaatatagat
    47821 acaaaccgat gattgctaca gtagttgttc gaatcccgcc gcctacagaa ctaggactgc
    47881 atccgataaa catcagtaaa gaaaaaatga tcagtgttgt gatttgaaaa tcacccaaat
    47941 cgtgaatctg cagaccagca ttacgagtcg taatcgaata aaacatcgaa ttgatccatt
    48001 tgacactcat attggaatct tggaataaat ggtccttttc caatagataa atcaagacag
    48061 ttcccgaaac aaacaagata acaaaagcta acacagctaa tttggtaaac aaggaaaaac
    48121 gaaacggaag cttcgcattg gaacgtttgt ataatagcca ttcccggcac tccatcaata
    48181 caggaaaacc aattcctccg acaaaaatca aaaacatgat caggatcaag aaaaaatagt
    48241 catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa cccagagttc gtgacagccg
    48301 atatcgcctg ataaaaaccg taaaaaacgg catcacgcca acgatcaaaa tagcctctgt
    48361 aataaaaata aatagaaaag aatgtcccaa aaagtaattg aaaccagatt aaaatagcaa
    48421 aagtgatccg aatcagtcgg acgataccag acaaacgcgg ttgattcata tccgtcatga
    48481 tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa aatgacaaaa gcagtagaaa
    48541 tcatcatgat ccccaaacca ccaacttgaa ataatatttc cagcagtata ataccgttat
    48601 cattgaacac agaattgata tcaaaagtcg atagtccagt cacactcact gtactgattg
    48661 ccatgaacag catgtccaca aaagaaacat ggctatctgg ttcgcgaaaa atcggcatat
    48721 aaaatagaac gagtgataag accgtcatca atatataata aaaaacgatg atttggatcg
    48781 atgagaaatg attggcagca aacttctgtc ctcgccgctg cagccttcga aagaataaat
    48841 caacatgcac agtactatcc tcctgacaat agtcgtttct accagtatac aaggggaatt
    48901 aggaatactc aaagaaatta tcaaacagat aacaatacag ctttcgatta aaatagaaat
    48961 ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag gaataacaaa ttgattaatg
    49021 ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa ttatgataca gttctgaaaa
    49081 aaatgatcat gcaatgggag aaagaagaag taaagcctaa gatattaata catacatgct
    49141 gtgctccttg cagtacgcac acattggaat tcatgtctca atatgcagag atcacgttat
    49201 ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt gcgaagatta catgaacaaa
    49261 aacgatttgt agaccaattt aatgagaaaa ctgaccatca tgttgcgcta atcgttgatg
    49321 aataccgtcc aaatgaattc accaaaatgg ttttaacaaa ccatcttgaa aacgaaaaag
    49381 aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt agatcttgtt gcgcaaaaag
    49441 cgcaagaact aggatacgat tactttggaa gtgccctgac catctcgcca aagaaaaatg
    49501 cagctttgat caatcagatc ggaatggata tccaaaagat ttacgggaca caatacttgc
    49561 caagtgattt caagaaaaac aaaggctatg aacgctcttt gcaaatgtgc aaagagtatg
    49621 atatttatcg ccaatgttat tgtggttgcg tgttcgctgc caagcagcaa ggatgcgatc
    49681 tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac atcaaccgta gtttttgagg
    49741 aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat aaagaattta tggtagaaat
    49801 aaggtagact gtataatttg tccgtagtaa agtaaggaaa caagaataaa tttcaatgaa
    49861 ggaagagata agatgcgaaa agtagcacct attttgaagc caatcgctgt attaacaatc
    49921 tcgacgattg tcgcgactac tacaggtatt gtgaatagtt ccagctaccg tgcaccaagt
    49981 tcaagttcta gtactgtaga agaaacaaca ccaactactt cagtctcgac atctgaacca
    50041 acaacaacgg aaagtttgaa tgaaaccact acgacaagta catgggacga tacaacaact
    50101 agttcgtcgt atgatgagac atatacaact gacagctcaa gcgaaacaag taatacggta
    50161 ccagatggca ctccatcgga ttcaaccaat ccaagtacaa gtccggatac aagtgctagc
    50221 tccgaaacga taccaagcag tacgactgta gatgataaca cgcaaaatga tgatgcagca
    50281 cgtacagcac agcaaaacca agattcagga aatacccaaa cgtataacca gcaagcaggt
    50341 gattctcaat gacattttta ctagacctta tcaacggggt gcataaattc cttggctatt
    50401 tagacatcag tcctaaatat cttaaccgtg gatatacgat tttaagtgtg attcccacgc
    50461 tttatttatt gagaattgtt tatggacttt ggcaaaacca aaactatctt caatttttct
    50521 tatatggact tgcatttcta gtgcttgttt attttactgt tttaaatgta ttttattact
    50581 ttttagataa aaacacgaaa gccgatgtca ctcagctttt tgtgaagtat ttacctgatg
    50641 aagcgttcaa catccagaca gaagaatcga tagataatgg aaattcgatc gataaagtga
    50701 atacacaaga ggtcatggtt tcttatgacg aagattatca attgaaatta gccgagaata
    50761 tgcggtattt gattggaaat ggtgagatca aaacaaatga actcggtcgc atggatggat
    50821 ttctggtcga tagaaataca ttgtatcctt attactatgt caagaaaaca ggagagaaag
    50881 agtatgttct aaatattgga cgaagctact cggacatgga aaaagtaggg accattcgac
    50941 ttgactcaac agaagacaca ttggaacctg tcggattgtt catcgtcggc ggtgattttg
    51001 tcaaagacgg gttacgctat catgagcctt atcgtctgaa attgattgca aaaaaggcac
    51061 agtcgcagga agaaccagtg gtttatagtc gtagccaaag aagaagacat tcataatgtg
    51121 atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta ttcggaataa gggggatgtg
    51181 acacgtcttt tgtcacgttc cttttttata taacgagaaa gataagacga acacatcact
    51241 tattctttcc tttcatttat aaacattgta gaataaagaa agtacaacga agtattgtga
    51301 aaaaacgtaa taaataacga ggaaaataca agtaggaggt aagctgtgaa agaatttttg
    51361 aaaaattggg ggattttgat ccttgtatta gcggctattt tattagctag agtgtacgtg
    51421 tttacaccag ttactgtaaa cgggcattcg atggacccta cattaagtga tggacaacga
    51481 ttgatttcat ctaagatctc aaactatgaa cgaatggata ttattactac aaaggaaccg
    51541 ggggatgaag aacggatgat tgttaaacgg atcatcggta tgcctggaga tacagtcaag
    51601 atggaaaatg accagttgac cattaatggc aaaaagtatg atgaacctta tctggatgag
    51661 ttcaaaaaag aattttcaga agataagtta caaggagaat atgcttatag ctcaggattc
    51721 caagcacaag cagaaagttc atccaccttt acgagtgact ttgaatatac tgtgccaaaa
    51781 ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa aagacagccg aatgtttggg
    51841 ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc gttattggcc tctttcagaa
    51901 attaaaatca tgtgatgaaa aaataaaaag aagctgtgac atgttttttg tcacagcttc
    51961 tttttccgaa aattccttct tatttcttgg agttgctcac tttttttgca atctcatttt
    52021 aagctttttg gctgatggat aagaaaagcg aagcgttttt gttcataaat agcttttccc
    52081 cacgatggaa ggtatgacta gtagcagaga caaagcccgt tttttccaat cgattattca
    52141 attcttcttg gctaaagcca ttatggactt tcggatgact aatttgttca ttcttgtcaa
    52201 agtctacgat gatcaattga ccgccaggag ctaaaatttc ataaagtttt gtaagaatat
    52261 tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag gatgagatta gcttggacag
    52321 caggaaattc gacagaagca tcagcgtgga tcgttcggac atttttcaga tctgctgctt
    52381 ggatcttttc ttctgccatt ttcagcatag tttctgaagc atcggcaatg atcagttctt
    52441 tgaatctttc cgcaagcggt aaactaacaa gcccagttcc gcctccgtaa tccagcagga
    52501 ctttttggtg gctgtcgcgc ggtagataag tagttagttc ttcgcgaata atcttggcta
    52561 atgcttgacg gtcaggggaa tcataatggt tagcgatgga atcaaaaata ttttttgtca
    52621 tcaaatagct ccttacgtaa aactttgaaa ttttaactta ttatagcacg tgaaaagaaa
    52681 gaaagcatgc tatactcaat tcaaaaaggg agaatctaga aagaatatgc cgatatcgtt
    52741 tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa atattactag acttgcctga
    52801 gtggtttggg cttcctgaat caacggaaaa atatatcgag gaatcaagta aactaccttt
    52861 atggtgtgaa aaaagaaaag aagaatattt aggctttatc actctttcac agacaagtga
    52921 agatactgcc gaaatctatt ctattgtatg ggaataaaaa aagactatca tcaccaagga
    52981 ttaggaacaa aactttttaa agaagcagaa ggttacgcag caaaacatta caaatatttg
    53041 caagtgaaga ctgtagacga ggggcactac tctatatatg atcaaactat ttgtttctac
    53101 gaatcattag gattttcaag gttggaagtt tttccaaatt tatgggatga atggaaccca
    53161 tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa tagatttaat actaatctgt
    53221 gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca agtataatac cataggaaca
    53281 aactggaagg tgaaaaagag catggcacaa ctatttttta agtatggcgc aatgaatagc
    53341 ggcaagacga tcgagatcct aaaagtcgca cataattatg aagaacaaaa taagccagtt
    53401 gtcttaatga caagcggtat tgatacaaga gatggtttag gcgttgtttc aagccgaatc
    53461 ggactgaaac gtaatgcgat gccgatattt gaagaaacag acgtatttga cgtaattcag
    53521 aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag cgcaattctt aaaaaagaaa
    53581 catgtcattg cttttacgag aattgtggac gaattggata ttccggttat ggcatttggt
    53641 ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa aatatttatt gctttatgca
    53701 gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc acaaaaaagc aatcatgaat
    53761 ttacattata ttgatggaaa ccctgtttat gaaggcgatc aagtgcaaat tggcggaaat
    53821 gaagcttatt accccgtttg tcgaaaacat tactttcacc catcaatgat tacagaagga
    53881 gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg ttatgaagag cttggagaac
    53941 ttttaagtga cccagaagtg atttctgata caaagcgttt tatgcagctt tcaaaagaag
    54001 aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta taaagaagta gtacaaggaa
    54061 tcaccgatac agaagaactg cttggtgaaa aactggatga tgaaatggct gaactagcaa
    54121 aagaagaatt atctgaatta aaaaaagaaa aagaagtctt agaagaaaaa atcaagatct
    54181 tgcttttacc aaaagatcca aatgacgata aaaatatcat catggaaatc cgtggagcag
    54241 caggcggaga cgaagcagca ttattcgctg gcgatctttt caatatgtac caaaagtatg
    54301 ctgaagcaca aggctggaaa acagaagtga tggaagctaa cgttaccgga atcggtggat
    54361 ataaagaagt gatcatgatg atcaccggag acaacgtata ttcgaaatta aaatatgaaa
    54421 gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc gcaagggcgt atccatactt
    54481 ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt agagatcgat attgaagaca
    54541 aagatatccg tacagatatc tatcatgctt caggtgctgg cggacagcac gtcaataaaa
    54601 cagcatctgc tgtacgtttg actcacttac caacagggat cgttgttgct atgcaagatg
    54661 aacgttctca aatcaagaac cgtgaaaaag caatgaaaat cttgcgtgct cgtgtgtacg
    54721 ataagatcca gcaagaagca caaagcgagt atgatgccaa tcgaaaatca gcagtcggaa
    54781 ctggggatcg ttcagaacgg atccggacgt ataatttccc gcaaaatcgt gtgactgatc
    54841 atcgtatcgg attaactatc caaaaattag atcagatttt agcagggaaa ttagatgaga
    54901 tcgttgatgc attgatcatg tatgatcaaa catcaaaact ggaagctatg caaaatgggt
    54961 aaaatgacct atcgagaagt cctgtcacgg gcttcttctt ttttagagga tcatgggaaa
    55021 gaagcatata gtatccaatt tttatttttg gaacgaaaac aatggaaaaa acttgattgg
    55081 ctccttcata tgaacgaaga gatttcagaa gaagaacagc ggttgatcga aaccgatctg
    55141 cgacttctgc ttgcagatca tccacctcag tatttgctag gatatgcaga tttttatgat
    55201 catcggttaa aggtaactga agcaacactg atccctcgac cagaaacaga ggagttagtc
    55261 gaatggtgtt tagacgagac gcctggtgta ccgttagaag tgatagatat tgggacaggg
    55321 actggagcaa ttgctattag tttgaaagca gcaagaaaaa attggcatat atctgctgtg
    55381 gatctttcag aagaagcatt agaagtagcg aaagaaaatg ctcagaaaga aggaacgaaa
    55441 atcagttttt atcatggaga tacattagga ccagtaatgg atcagcaatt tgatgtgatt
    55501 atttctaatc cgccgtacat cagtcggaat gaatgggaac tgatggatga aagtgtccgg
    55561 tcattcgaac ctaaaatggc cctttttgct gagaacgatg gattagctat ttatgaaaaa
    55621 atagcagttg aagcaccaag cgtactaaaa tcaaatggaa agattttttt agaaatcgga
    55681 tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag catttccaga taaaaaagtg
    55741 gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc aagtttatgg ttgaagttgt
    55801 ggatgaaaaa ttttttattt gtttttttat tataaaaaaa cacttatttg acaaggatgt
    55861 aaacgacttt ttggtttatc aacaagttta tccactattg acaaatagat tatccacaaa
    55921 cagtggaaaa cttgaggata aactcagaca aaataagaat gtatgaaaaa ggggttgtgg
    55981 ataaactgtg gaaacgaaac gatatgatga aacagaattg caagaggcag ccgaagcact
    56041 gaaagaagga gaacttgttg cttttcctac cgaaacagta tacggattgg gcgccaacgc
    56101 tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa gggagaccgc aagataatcc
    56161 attaatcgtc catgtcgcct cttttgaaca agtaaaagag tatgtggaca actttcatcc
    56221 ggcagcagag aagattgtga agaacttctg gcctggacca ttaactttga tatttaagat
    56281 caaaaaagac actttgccct cagttgtgac gggaggatta tccacagctg catttcgtat
    56341 accggataac aaaaaaacac tagaagtaat cgaactttcc ggtgttccgc ttgtcggtcc
    56401 aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca gatcatgtat atcatgatct
    56461 acaaggaaaa attaccggga ttattgatga tggagctacg cgaattggtg tagaatccac
    56521 tgttcttgat ttaagtgatc caacagccat gcctatgatt ttacgaccag gtgcagtaac
    56581 gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct attgatcagc atcttgtaaa
    56641 agaaaatgaa acaccaaaag cccctgggat gaaatacaaa cactactcac ccgatacacg
    56701 tgttctgatg gtcagagatg gcgattggtc aactgcagtt caatgggcaa aaaataagaa
    56761 gatccgtgca ggtgtcatag caagccccga aattgcggat caagtgcgga cagatactgc
    56821 agctgtttat atgtataacg acaactcagt agaagcggcg gctaaaggat tgtttgcagg
    56881 tctaagagga ttagatgaac caacacttgg attagatttg atttttgtac aagtgtatcc
    56941 agaaactgga ttggggaatg cttacatgaa tcgcttgaaa aaagcagcag gacaaaatta
    57001 ttttgaaaaa tgatcaattg gttattgtcg cattctttta gtatgataca atgctatcaa
    57061 gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta atcgtaatag aagagatacg
    57121 aatatttggc aatttgtttt tgatgggaaa aataaaattg taggaaaata aaaaggagct
    57181 gttgtttgtg gtagattaca aaacgtttga ccctgatctt tgggctgcga tcgccaaaga
    57241 agaagaaaga caagaacata atttggagct gattgcctct gaaaactttg tctccgaagc
    57301 agtgatggct gcacaaggaa gtatcttgac aaataaatac gcagaaggtt atccagggca
    57361 tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa aatttagcga ttgaccgtgc
    57421 aaaagaatta ttcggagcga aatttgcgaa cgtccagcct cactccggct ctcaagccaa
    57481 cacggctgct tatctcgctt tggtagaacc aggggacacg atcttaggaa tggatctttc
    57541 cgcaggcgga catctaacac atggatcacc agtcaacttc agtggcaaaa cctatcactt
    57601 tgttgcttac ggtgttgatc cgacaacaga agttattgat tataatgtgg tacgtatttt
    57661 agctagaaaa catcaaccaa aattgatcgt agcaggtgcc agtgcttacg gacgaacgat
    57721 cgactttgcg aaattcagag aaatcgcaga tgaagtaggc gcaaaactga tggtagatat
    57781 ggcacatatc gcaggattag tagcagcagg acttcatcct aatccagttc cttatgcaga
    57841 tatcacaacg actaccactc ataaaacatt gcgtggccct cgaggcggca tgattttgac
    57901 aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta tttcctggta ttcaaggcgg
    57961 ccctctagag catgtgattg cgggtaaagc agtagcattc aaagaagcat tagatcctgc
    58021 atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa gcaatggtca aagttttcaa
    58081 tcaagcaatt ggaaccagag tcatttcagg ggcgacagat aatcatctga tgttgatcga
    58141 tgttcgcgag ttaggtataa atggaaaaga agcagagagc attttggaca gtgtgaatat
    58201 cactgtaaat aaaaactcga ttccttttga aacactaagt ccattcaaaa caagcggtat
    58261 tcgaatcggc acgccagcaa tcactactcg cgggttcaaa gaagaagatg ctgtaaaagt
    58321 ggctgaacta gttgtcaaag cattacaagc aaaagatgat aacgctcaac tagacgaagt
    58381 caaaacaggt gtacgcgaat taactgaaaa atttccgttg cataaaaaat aaaaatttaa
    58441 attccattag taaacagaaa agttcaatgt tgggcttttc tgtttatttt ttgggaaaac
    58501 ggattctttg acaatatcgg tgatgctctc tagtatatga ggtgtaattt tagtcttcac
    58561 actaaatctc agggaggaaa agtcatgact gatcgtatct cgttagaaaa ggaagcagta
    58621 gcattcagtg atgcaaatgc gcctcatccg cgtatctatg aactagcacc ggaagaagga
    58681 agagttttac tagaagaagt acaagaatca cctgtgaaca aatattccgt cgatattgaa
    58741 gacttgatgg tggatactgg cgaatgggga aaactcaata ttcgtctcgt acgaccagaa
    58801 gggaatatgg ataaattacc agttattttt tatattcatg gagcaggctg ggtttttggc
    58861 agtgctcaaa cccatgataa attgatcaga gaacttgcgg ttcgtacgaa ttccgtcgtt
    58921 gtctttccag aatatagtcg atcaccagaa gcaaaatatc caacagcaat tgaacaaaat
    58981 tatgctgtgt tgcaacagct gaataaatta gccaagtcaa aaaatctgga tctcgagaat
    59041 ctaatcgttg caggagattc agtaggtggt aacatggcta cagtcatgac gatcttaaca
    59101 aaagaacgaa agggcctccc aatatccaag caattgctgt attatccagt cacaaacgct
    59161 gattttgata cagattccta tcatgaattc ggagaaaact attttttgac aagagaagga
    59221 atgatgtggt tttgggacca atatacgacg aatgaagaag agcgtgctga aatcacagct
    59281 tcgcctttga gagcaaatat aaatgaacta aatgatcttc cttcagcaat gattttaacg
    59341 ggagaagcgg atgtattaag agatgaaggt gaggcttatg cgcgcaaact tcgtgatgca
    59401 ggggttcctg ttacacaagt acgcttccaa ggaatcatcc atgattttgt catggtaaat
    59461 gctatggatc agacaaaggc aacgcgtgca gcaatggcat tatctacttc ttggatcaag
    59521 aaaaaagaat aagctttgct tgtccgcagg aaaacttctt ttcaaaaacg gattttctta
    59581 ggaaaaatgg cgagtttgac tagtcaatca cttgattttg ttttacaatt aaaaagaaac
    59641 gacagaacaa aggagagctt aaaaaatggg caaatttcaa gtgatcgatc atccattgat
    59701 tcaacacaaa ctaactatta ttcgggacaa aaactgcgga acaaaggttt ttcgtgaagt
    59761 agtcgatgaa atcgctatgt tgatggcata tgaagtatca cgggatatgc cattggaaga
    59821 catcgtgatc gaaacaccga tcactgaaac aacacaaaaa acgttgtctg gtaaaaaagt
    59881 agcgattatt ccaatcttgc gcgcaggtat tggaatggtt gacggtatct tggaattgat
    59941 tccagctgct aaagtgggac atatcggtct ttatcgagat gaagaaacat tggaaccaca
    60001 tgaatatttc gtcaaacttc cagaagacat tgatgcacgt cagttgtttg tagtagatcc
    60061 aatgttggca acaggcggat cagcaatcat ggcaatcgat gcattgaaag aacgtggcgc
    60121 gtcgaacatt aaatttgttt gtttggtagc agcacctgag ggagtaaaag ctttgcaaga
    60181 agcgcatcct gatattgaca tctatacagc ttctctagat gaaaaattga atgaacatgg
    60241 ttatatcgtt cctggactag gggatgcggg tgaccgtttg tttggtacaa aataagtaaa
    60301 tacgaaaaag ggtgtgacac aacttttgtc acatcctttt ttggtcattt ttaacgtttg
    60361 atgcagaaca gctttttcac agctttcgtt tgcatataaa aaaaccatcc caaaaatgca
    60421 gagatggtca aattcaaatc ttttttagtt ctttgtagtt aacgattact acacccttac
    60481 tttgcgtcat caagttcgca ttttctgcag tttcatcaat ctcaacaaca gcggaatttt
    60541 tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg aattgtgaac attacgtgag
    60601 tattgatctt gaatttttca ccaatttccg atggtacgat agttgtatca aattttccaa
    60661 aagttgccat tttcataaaa cctgcctttc acatcaatta taccacgttt tatgaaaaat
    60721 atcatgaaat taaactaatt ttccatcgga ctctttacaa tcagccaaat agagtatata
    60781 atttcttttg gataaggcca gcttagctca gttggtagag caacgcactc gtaacgcgta
    60841 ggtcgtaggt tcgatcccta tagctggcat taaaagagaa gacagatata tcaaggattt
    60901 ttaatccatt gatacatctg tctttttgtt taaaggaaat aaagtatgct ttcttagcaa
    60961 tagagcatgt attatttcta taaaaattta ggagaataaa acgactagca atatagctgt
    61021 gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc gagttgcttt taatggcagc
    61081 ttggttgaat aacaatcttg ccaccccttg tatcaaaagg atgtgtgcta cagtgtgtaa
    61141 aatatttcca ttatcataag tactgtaatg gataaatcca aagatgattg ctgacaagaa
    61201 actagcgaca ggaatgctga acttactaga aaaaatagcg agaaaataca ctgagaacaa
    61261 ttcttctcca agtatcatga taggcaaaat aataaataat aacgggctga aagcctcatt
    61321 tattggattg cctgttaaac cccatttcaa aatttgttgc aaaaataccg agactgcggc
    61381 actgattact acattgataa aaaaatattt ggctatattt ttgacaggag cgacaggttt
    61441 cgagaacatt tttttcgtat cttttttacc aaaaacaagt gtaaatgcta taaatccagt
    61501 gatagttaaa gaaaaaatgc taataatgga tggcgcaaat agcaacccta acaaaccgag
    61561 tacaacgaca atagtgccca atatcccttt tttaagttct attttttttc ctaaaatgtt
    61621 attcatgata ataactccta ctattcagtt tttgaagata gagtcagtat acatgaagag
    61681 ttaaaatagg caatgttttt ttatggatat ttgtattgct aaaaatttat cttatatatt
    61741 cattttgatt atactacttt tatttgtaaa aattttctag caaaaccaag tgcttgcaac
    61801 tgattaagcg tacaattttt tagatgttcc gagcgtactt caatcatttc aaaggagttg
    61861 ctacttaaat cgatattttt taaatcagta tcaaaaaagc tagcattttc taaatcatta
    61921 gtagaaaaca ttattttagt gagggaacag tctacaaaat ctgcgcttga taagacagag
    61981 tgctcaaagg tactttcttt tatgtttgaa ccagataaat ttacatagtc cattttacaa
    62041 ttctcgaata acgtgttccc aatataactt tccatcaatt tagtacctgt taatttacaa
    62101 ttgacaaatt tcacgcgatg caacatgctt tttgtcaata tacagcctga aaaatcacag
    62161 ttttcgaata tacagtcggt aatctccatt ctttgaaaat cattggaagc aaaaaatgca
    62221 tacttgaaaa tagatttgta aataattgga tggcagatta tttggttttc gatgacctct
    62281 ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt catagatatg aaaaaaggat
    62341 cctttattca agcttgcttc attaacttta ggaaaaatca tatttaatta cctccatttt
    62401 cataaagtca tattaacaaa agctaaacca acttacatta gcagaaaatg agataggttt
    62461 tcgccttttt cgataacgat agtgtcgcta tctcttgaaa cgttcttaga attgccctga
    62521 gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa taaaaatttt tactgattta
    62581 atttagaatg tttttaaaat aaaaatcgtt gccataacag tgtattgaaa ggttttaaca
    62641 atccagtaat gatttaaaaa aatctagatg tagcgtatac tttgtttata gaatgtagtg
    62701 ggaagaaata tatttttggg aggaaatcgt gatgtctaaa agtgaagaaa tagaaaacat
    62761 ggtactaaaa ttatatgatc tcgttttaaa tccagagatc aaagaaaagg aacgtatact
    62821 tttgatagac gcaaagacag gcttagaaaa aggtcagtat tatcctaaag tcataaataa
    62881 tttggaacgt agtttacgtc cgttagcaat caggggagaa ttgtctaaac cagtatcacc
    62941 gttttatatg gagatatcaa caataggaaa atttgaaaaa gaattaggtc gaggaatggc
    63001 ttcggcaccg attacttttg ggcatttata aaaacgagtt attcatctca atgaattaaa
    63061 aagtatagtc aatctcataa tgcaggctag gttcatttat ttgaacagag tctgtatttt
    63121 tttattctaa ataaagaaac agatgtttct ttatattgta ttttatcctt taattaacac
    63181 aaaaacagct agt
//
LOCUS       DACDVT010000005        62228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDVT010000005 DACDVT010000000
VERSION     DACDVT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.005"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            71..805
                     /locus_tag="IWL52_000299"
     CDS             71..805
                     /locus_tag="IWL52_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9776891.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            844..1986
                     /locus_tag="IWL52_000300"
     CDS             844..1986
                     /locus_tag="IWL52_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9776892.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2081..3472)
                     /gene="sufB"
                     /locus_tag="IWL52_000301"
     CDS             complement(2081..3472)
                     /gene="sufB"
                     /locus_tag="IWL52_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9776893.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(3477..3947)
                     /locus_tag="IWL52_000302"
     CDS             complement(3477..3947)
                     /locus_tag="IWL52_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9776894.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(3934..5169)
                     /locus_tag="IWL52_000303"
     CDS             complement(3934..5169)
                     /locus_tag="IWL52_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9776895.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(5166..6452)
                     /gene="sufD"
                     /locus_tag="IWL52_000304"
     CDS             complement(5166..6452)
                     /gene="sufD"
                     /locus_tag="IWL52_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9776896.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(6465..7238)
                     /gene="sufC"
                     /locus_tag="IWL52_000305"
     CDS             complement(6465..7238)
                     /gene="sufC"
                     /locus_tag="IWL52_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9776897.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(7435..8283)
                     /locus_tag="IWL52_000306"
     CDS             complement(7435..8283)
                     /locus_tag="IWL52_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9776898.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(8307..8993)
                     /locus_tag="IWL52_000307"
     CDS             complement(8307..8993)
                     /locus_tag="IWL52_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9776899.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(8986..10020)
                     /locus_tag="IWL52_000308"
     CDS             complement(8986..10020)
                     /locus_tag="IWL52_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9776900.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(10415..10753)
                     /locus_tag="IWL52_000309"
     CDS             complement(10415..10753)
                     /locus_tag="IWL52_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9776901.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(10755..11111)
                     /locus_tag="IWL52_000310"
     CDS             complement(10755..11111)
                     /locus_tag="IWL52_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9776902.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(11117..12304)
                     /locus_tag="IWL52_000311"
     CDS             complement(11117..12304)
                     /locus_tag="IWL52_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9776903.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(12495..13340)
                     /locus_tag="IWL52_000312"
     CDS             complement(12495..13340)
                     /locus_tag="IWL52_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9776904.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            13574..14539
                     /locus_tag="IWL52_000313"
     CDS             13574..14539
                     /locus_tag="IWL52_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9776905.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            14916..15959
                     /locus_tag="IWL52_000314"
     CDS             14916..15959
                     /locus_tag="IWL52_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9776906.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16021..16485)
                     /locus_tag="IWL52_000315"
     CDS             complement(16021..16485)
                     /locus_tag="IWL52_000315"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9776907.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            16731..17678
                     /locus_tag="IWL52_000316"
     CDS             16731..17678
                     /locus_tag="IWL52_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9776908.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(17889..19961)
                     /locus_tag="IWL52_000317"
     CDS             complement(17889..19961)
                     /locus_tag="IWL52_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9776909.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(19894..20271)
                     /locus_tag="IWL52_000318"
     CDS             complement(19894..20271)
                     /locus_tag="IWL52_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9776910.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(20400..20960)
                     /locus_tag="IWL52_000319"
     CDS             complement(20400..20960)
                     /locus_tag="IWL52_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9776911.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            21110..22402
                     /locus_tag="IWL52_000320"
     CDS             21110..22402
                     /locus_tag="IWL52_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9776912.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(22462..23580)
                     /locus_tag="IWL52_000321"
     CDS             complement(22462..23580)
                     /locus_tag="IWL52_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9776913.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(23594..25015)
                     /gene="arcD"
                     /locus_tag="IWL52_000322"
     CDS             complement(23594..25015)
                     /gene="arcD"
                     /locus_tag="IWL52_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9776914.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(25261..26382)
                     /gene="mnmA"
                     /locus_tag="IWL52_000323"
     CDS             complement(25261..26382)
                     /gene="mnmA"
                     /locus_tag="IWL52_000323"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9776915.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            26786..27385
                     /locus_tag="IWL52_000324"
     CDS             26786..27385
                     /locus_tag="IWL52_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9776916.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(27523..28809)
                     /gene="efmA"
                     /locus_tag="IWL52_000325"
     CDS             complement(27523..28809)
                     /gene="efmA"
                     /locus_tag="IWL52_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9776917.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(29080..29424)
                     /locus_tag="IWL52_000326"
     CDS             complement(29080..29424)
                     /locus_tag="IWL52_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9776918.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(29561..30697)
                     /locus_tag="IWL52_000327"
     CDS             complement(29561..30697)
                     /locus_tag="IWL52_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9776919.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(30886..31860)
                     /locus_tag="IWL52_000328"
     CDS             complement(30886..31860)
                     /locus_tag="IWL52_000328"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9776920.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(31940..32815)
                     /locus_tag="IWL52_000329"
     CDS             complement(31940..32815)
                     /locus_tag="IWL52_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9776921.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGCM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            32945..34852
                     /locus_tag="IWL52_000330"
     CDS             32945..34852
                     /locus_tag="IWL52_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9776922.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(35393..38671)
                     /locus_tag="IWL52_000331"
     CDS             complement(35393..38671)
                     /locus_tag="IWL52_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9776923.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(38942..39487)
                     /locus_tag="IWL52_000332"
     CDS             complement(38942..39487)
                     /locus_tag="IWL52_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9776924.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(39499..40191)
                     /locus_tag="IWL52_000333"
     CDS             complement(39499..40191)
                     /locus_tag="IWL52_000333"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9776925.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(40210..40494)
                     /locus_tag="IWL52_000334"
     CDS             complement(40210..40494)
                     /locus_tag="IWL52_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776926.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(40507..41070)
                     /locus_tag="IWL52_000335"
     CDS             complement(40507..41070)
                     /locus_tag="IWL52_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9776927.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            41298..41960
                     /locus_tag="IWL52_000336"
     CDS             41298..41960
                     /locus_tag="IWL52_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9776928.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            42084..43280
                     /locus_tag="IWL52_000337"
     CDS             42084..43280
                     /locus_tag="IWL52_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9776929.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(43381..44757)
                     /locus_tag="IWL52_000338"
     CDS             complement(43381..44757)
                     /locus_tag="IWL52_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9776930.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(44794..45660)
                     /locus_tag="IWL52_000339"
     CDS             complement(44794..45660)
                     /locus_tag="IWL52_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9776931.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(45749..46774)
                     /locus_tag="IWL52_000340"
     CDS             complement(45749..46774)
                     /locus_tag="IWL52_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9776932.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(46792..47466)
                     /locus_tag="IWL52_000341"
     CDS             complement(46792..47466)
                     /locus_tag="IWL52_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9776933.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(47650..48543)
                     /locus_tag="IWL52_000342"
     CDS             complement(47650..48543)
                     /locus_tag="IWL52_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9776934.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(48617..50314)
                     /locus_tag="IWL52_000343"
     CDS             complement(48617..50314)
                     /locus_tag="IWL52_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9776935.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(50307..51620)
                     /locus_tag="IWL52_000344"
     CDS             complement(50307..51620)
                     /locus_tag="IWL52_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9776936.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(51620..55138)
                     /locus_tag="IWL52_000345"
     CDS             complement(51620..55138)
                     /locus_tag="IWL52_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9776937.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(55431..55889)
                     /locus_tag="IWL52_000346"
     CDS             complement(55431..55889)
                     /locus_tag="IWL52_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9776938.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(56186..56704)
                     /locus_tag="IWL52_000347"
     CDS             complement(56186..56704)
                     /locus_tag="IWL52_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9776939.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(56706..57323)
                     /locus_tag="IWL52_000348"
     CDS             complement(56706..57323)
                     /locus_tag="IWL52_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9776940.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(57320..58174)
                     /locus_tag="IWL52_000349"
     CDS             complement(57320..58174)
                     /locus_tag="IWL52_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9776941.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(58171..59082)
                     /locus_tag="IWL52_000350"
     CDS             complement(58171..59082)
                     /locus_tag="IWL52_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9776942.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(59123..60457)
                     /locus_tag="IWL52_000351"
     CDS             complement(59123..60457)
                     /locus_tag="IWL52_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9776943.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            60665..61495
                     /locus_tag="IWL52_000352"
     CDS             60665..61495
                     /locus_tag="IWL52_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9776944.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(61739..61988)
                     /locus_tag="IWL52_000353"
                     /pseudo
     CDS             complement(61739..61988)
                     /locus_tag="IWL52_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        17777 a        13277 c        11215 g        19959 t
ORIGIN      
        1 aacatctttg taaaatttat ttacaacacc ccttttttga ggtatactaa ttttaggagg
       61 tgtggtcaat atgataaaac gtccaccaat aaattattta gaacgaaaaa agatactcgg
      121 aacaaaaata aaagctataa gaaagagtaa aaaattaaca caaccagcat ttggtcttat
      181 gattaacaac ggtcaattaa ttgacaaaaa aactatctac gaatgggaga agggaaccta
      241 tttaccaata ccagagcgat tatcccgaat tgctgatttg ggaaatatga gtatagagga
      301 gttagtttgt ggcaatgtag aagaatatat tttgggaatt attctttata gagatagcat
      361 tgtactagat ggtataacat ttccagataa aaacctgttc cagcatcttc gtcaacagtt
      421 cccaccagta cactctaatt tagatacatg gctggataga tattccaaat tagaaccaga
      481 gatgcaagaa tttattgcta acaagacttg taataaagtt aaaaatgaaa aaattagtct
      541 tttcaatatc ttaaaaatag aagaattatt catcaatgct attgtagaaa aatttgacaa
      601 taacattctt ttcctcacta gcagtataga agaactacta gaaagaatgg tagatgaatg
      661 gctacctatt caattgaaag acatgtctta tcccgaagaa gctgtgagag aaattactga
      721 taatataaac aagctagaac aaacgatttc atcaattggg aaaaaataca ctaagaaaaa
      781 aatgaaggga ggtgatacca tctagtggta agtactcctt gtaaactaag aggagatgat
      841 ttaatggcaa aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa
      901 ggacacattg ccaccgatcc acgaactggt gaaaaaataa atactacgag aagaggtttt
      961 agtagcaaac aagaagctca aaaagattat gacgagtaca gacatcaaat attatacggt
     1021 gttaaaaaga aagcacctga tatgacattt gaagacctgt ataatgaatg gataactcat
     1081 cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag taagatacgc aaataatcaa
     1141 atacttccag catttggtaa gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa
     1201 gtggtagatg aatggcacag taaatatgaa agttacgatt atatgagaaa acagactgca
     1261 caaattttac gctacggggt tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca
     1321 cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa agaggagctt
     1381 aataatttac tggatgcttt taaagatttt ggtaacatga aacaatacgc cttcttcaga
     1441 ttattggcat acacaggtat gagaaagagt gaagtactcg ctttacagtg gaaagacata
     1501 gacacgttta ataaagaact acatgtcaat aaaactcttg ctgtagatga atttggtaaa
     1561 gttataattc aatctccgaa aacaagagct tctagacgag ttatctcttt agatactgag
     1621 actctttcta tcctcaacaa ctggaaatta cagcaaaaag aagagtattt aaaacttgga
     1681 tacaatacta gctctaaaga gcaacatgtt tttacaacag ttaaaaacac tctatacatt
     1741 ccaaacactg ttaatgattg gttaagatat attttgaaaa aatataactt gcctcgtatt
     1801 acacctcatg ggtttagaca tacacatgct agtttattat tagaagcagg agaatctgtc
     1861 aaggtagttc aacaacgatt agggcatgaa aatagtaaag tgacattaga catctatgca
     1921 catattacaa ataatgcacc taagaaaaca ggtcaagact ttgctgatat gatggctcat
     1981 cagtaaaaga gctggtcaaa atctggtcat ttctagtata caggcaaaac aaaaaagcta
     2041 gaacccttat atatcaaggg ttctagctcc tataacaggt ttacccaaca ctgccttcca
     2101 tctcataact gatcaatcga ttgagttcga ctgcatattc cattggaagt tctttggtga
     2161 atggttcaac aaagcccatg acgatcattt cagttgcttc agattcggtc aggcctcggc
     2221 tcatcagata gtagagttgt tcttctgaga tttttgagac tttggcttcg tgttctaaag
     2281 agacttggct gttgtggatc tcattgaatg ggatcgtatc ggatttcgac cattcgtcca
     2341 tgatgatcgt gtcacattcg atatgagaga tcgaacccgc actgtttttg gcaaaggtta
     2401 cttgtcctcg atagtttact tctccgccgt cttttgaaat tgatttagag acgatagagc
     2461 tagatgtatt tggtgcattg tggatcattt ttgcacccgt atcttggatt tggtttgctc
     2521 ctgcaaaggc gatggagagc atggtgcctc gtgcaccttt tccatctaag taaacacttg
     2581 ggtatttcat cgttgttttt gcacctaggt ttccgtcgat ccattcaacg gttgcgcctt
     2641 cgtatgcttt tgctcgtttg gttactaagt tgtacacatt gtctgaccag ttttggatcg
     2701 ttgtgtaacg gcaatacgca tcttttcttg taaagatttc tacgatggcg gcatgcaggc
     2761 tgttacttga ataagtaggt gccgtacaac cttcgacata gtggatgctt gctccttcgt
     2821 caacaatgat cagcgtacgt tcgaattgcc ccgtgttttc cgcattgatc cggaagtacg
     2881 tttgtaatgg aacatcgact ttgacggctt ttggcacgta aataaaggtc ccacctgacc
     2941 atacggctga attcagcgct gctaatttgt tgtcggttgg cggtacaagt tttgaaaagt
     3001 actctttgaa taattcaggg tattctttca aagcagagtc agtgtcagta aagacgattc
     3061 ctaacttttc gaattcatct ttcatattat ggtacacgac ttctgattca tattgtgcgg
     3121 aagcaccggc tagataagct cgttcagctt ctggtatacc aatcttttca aatgtatctt
     3181 tgattttttc tggtacgtct tcccagtcac gagcgggttt gtcacttggt ttttggtaat
     3241 atttgatggc atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc attggcattt
     3301 tgttaaatgt ttctagagat ttcagacgga attcaagcat ccattctggt tcgcctttga
     3361 ttcttgacat ctcacggatc acttcttctg tcaagccttc tcctgtactg aaaaccggtt
     3421 cgacatcatc gtgaaacccg aatttatatt cttctaattc tggtacgccc atgttctcac
     3481 tcctcttcct tgtggtaatg atggaaagct gcggtaccat cgttttccat tgcttttcct
     3541 agtgctttcc aggcaagtgt cgcacatttg atccgagcgg gaaatttagc tacaccacca
     3601 agcatagcag catcgcctaa ttggtcggga tctttgactt cttttccttg gacaagctgt
     3661 agaaagtcta cagttaaatg ttctgcttct tctattgatt ttccgatgac ggcgtctgtc
     3721 atcatcgagg cacttgccgt actgatggag cagccactgc cgtcaaaggc gatatttttg
     3781 atgattccgt cttttacatc gacttgcaat tcgatcacat ctccacatgt ggggttgttc
     3841 aattcgatct tgcttgtcga gtcagtcaag cttccacgat gatgcggatg ggaagaatga
     3901 tccaagatca cttgacggta taaattgtct aatttagata gtcccatgtt ggaaaaactc
     3961 ctttgttgcc atgattgctt caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata
     4021 aaagctcgcg cgagcagtcg ctgatacttc aaggtacttc aatagcggtt gcgcacaatg
     4081 atgacctgct ctgacggcta ccccttccat gtctaaagct gtcgcgacat catggggatg
     4141 taacccatcc aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt gtggaccata
     4201 aacagtcaat ccatcaattg cttgtaattt tggcaacaca taagcggcta gttcttcttc
     4261 atatcgatgg atattgtcca taccgagttt ttgcaaataa tcgattgcat ggcctaatgc
     4321 gatagcgccg gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt
     4381 actgtcgtat aattcgacaa aatcgatcat ttcgccacca aattcaacag gttccatttc
     4441 ttctaaccat tttcgtttac catataaaac accaatacct gttggtccgc acattttgtg
     4501 tccgctgaaa gcaaagaaat cacagtctag tgcttggaca tcaatggcca tgtgcggggc
     4561 agattgagca ccatctacta cgatgacccc gccgttttca tggacaagtt cagctaattc
     4621 ttgaacagga ttgaagacac ctaagacatt ggatacatga gcaatggaga cgattttggt
     4681 tttgtccgta atctttttcg ctgcatcctc catatccagg aagccttctt ctgttagttc
     4741 gatatatttc aagacagcac ctgttcgttt tgccaactgc tgccacggga taacattaga
     4801 atgatgttcc atgtaagaga gcactatctc gtctcctgct ttgatatagc ggtcgccgaa
     4861 ggttctggct acccaattca agcttgtcgt cgtaccacga gtaaacagca cttctgctgt
     4921 ttcttttgca tggatgaacg cccgtacttt ttctcttgct tgttcataat catgggtcgc
     4981 gcgttctgct aacgtatgga caccgcgatg aacattggcg ttatcgtgtt cgtaatagta
     5041 cgctaatgcc tggagtactt gttttggctt ttgggtcgtc gctgcattat ccagataaac
     5101 taaaggttca tcattgacgt cttgaaataa gataggaaaa tcattgcgta gctttttagc
     5161 atcgatcatc cattcaactt cccttcgatt acttcgacta attctttccg aacttgttct
     5221 acagggattg ctgttaagac agaaccgagg aatcctcgaa tcactaagcg ctctgcttct
     5281 tctttatgca atcctctact catcagatag tacatttctt ctggatcaac tcgtcctaca
     5341 ctggcagcat gtccagcagt tacttcgttt tcatcgatca aaagaatagg attcgcatca
     5401 ccacgtgctt tatcagaaag catcaagaca cgactttctt gttgggcatc tgcacctttt
     5461 gcccctttca agatgtggcc gataccatta aaggtcaatg ttcctttttc tcggatcact
     5521 ccgtgttgca aaatatgccc gatcgtatga ggggctttat tggttactcg tgtatcgatt
     5581 ccttggattt gtctgcccga gctgattgcc acgattttga cttctgcgtg tgcaccttct
     5641 cctgctaaat ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac accaattgcc
     5701 cagtcgacag aagcatctcg taaaatgtgc ccacggcgat tcatataaga agtaatattt
     5761 tctcctaatt gatctacggc tgaatatttg atttttgaac cagctttagc gatgacttcc
     5821 acgatgatat tacctgatga ttttgctact tgttctttgg ttgtctggaa acgttccaga
     5881 taagaaaatt cactatgttc atctgccacg atcaacacat gcttgaagaa gtgctcatcg
     5941 gatgcgccat cttgaatgaa taaggactcg agtggttcat cgatcacgac atttttaggc
     6001 acatacagga aaataccgct gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg
     6061 ttcatgttta ctgctttttt catataatat tcttggacta gttctggata ttcgatcatt
     6121 gcagtgaaca agtcggtaaa aatgactcct ttttctgcta attccacagg cagttgttca
     6181 aaaatcgtcc atgaaccttg ttggacaagc acaggattgt ctttcattgc atcaaatgcc
     6241 ggaatcgtgc ctgtttcact gatggtttgt gtttctttca catcaaataa cgaccagcga
     6301 tggaatttca cccgatcgat atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct
     6361 tgacggaagg ttctcatcca atcaggttcg cctttactta aagaaaaatc atttaccgcg
     6421 tcaagataat cgagccagtt tttttctttc attcttgctc ccccttacgc ttcttcttct
     6481 ttatagtcga ttcctaattc tttactgata ccagcgtagc cttctgcttc cagacgttta
     6541 gctaggtcag cattccctgt catgactact cggccttcca tcatgatgtg aacgacatca
     6601 ggtgtgatat agttcaataa tcgttgatag tgggtgatga tcaatgcacc gaaattgtct
     6661 ccgcgcattt catttactcc tttagcaacg actttcaaag catcgatatc cagacctgaa
     6721 tcgatttcgt ctaaaattgc aaatgtaggt tctaacatca acaattgcag gatctcattc
     6781 cgtttctttt ctccgccaga aaaaccttcg ttcagataac gttcagccat ttcttctggc
     6841 atatttagta aagccatttt ttcatctaac tttttcaaaa atgccataac agaaattttg
     6901 tcgttttcat cacgtttcgc attgatcgct gcacgcatga attccgcatt cgtgatacct
     6961 ggtatctcac ttggatattg catagctaaa aataagccaa gtcgcgcacg ttcatctact
     7021 tccaagtcta aaacattttt cccgtcaaac aaaatttcgc cttctgttac ttcatagttt
     7081 ggattgccca tgatagcggc agataaggta gattttcctg tgccgttagg tcccatgatg
     7141 gcatggattt ctccggtttt cattgttagg tttacgcctt taaggatttc tttttcctcg
     7201 atcgacacat gtagattttt tatttctaag acagacatgc attatacccc caaattctct
     7261 ttcgttctca ctcattttag actaattgag aatgaagcgc aatgaccttc cttactaaaa
     7321 tcaaatatct tttatttatt attaaacaat tttgatttct aaattagaat aggtataaaa
     7381 aagatgagaa ataagccatt acagacttat ttctcatctt ttttgttcca taccttattt
     7441 tgcatcagcc tttactggtt taacagagcc gccccatttg tctgtgatcc attcttggac
     7501 ttctttgcgg tgaagcactt caatcagttt tttgattttg tcactgtctt catcaccttt
     7561 acgagtcgca ataatattca cgtaaggaga gttgtccttt tcaagcaata cggcatcttc
     7621 taatggattc aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta ggtcaccttc
     7681 atcattttgg tacgtagaag ctaaaagtga tgggtcgatc gtatggttga acttcaagtt
     7741 cttagggttt tcttcaatat catcgaatgt tgctgtttcc agatctactc catctttgac
     7801 tttgactaag ccagcatctt gtagaattgt gatgatgcgt ccccagtctg attcagaatt
     7861 tgaagtaatg attgttgcac catcttttaa ttcggaaaca tctttgactt ttttagagta
     7921 caagcccatt ggttcgatgt ggacagcacc ggcattgaca aagtcatatc ctttttcttt
     7981 gatttctttg ttcaaataag gaatatgctg gaagtaattg gcatcaatgt cgccttcttc
     8041 caaagattgg ttcggtaata catagtcatc gaatttgacg atttcaagat cgattccttc
     8101 atctttcaat aatggtttca catgttccaa aatctctgcg tgcggtgtcg gtgaagcgcc
     8161 gactttcaat gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc tgttgttacc
     8221 gcatgctgct aatcctacag tcagcaaaag cgctgctgca aatccaaaaa ttttcttttt
     8281 catatcctac atccccctat atcctattat cttttgtctg ttttcttagt taagaaatca
     8341 ccgattcctt gaacgataaa tacgattagt aaaataatta aagtcgcaac gaatgtcact
     8401 gttagattac cacgctggta gccttcctgc caagcaagac ctccaagtcc acctgcgccg
     8461 atcgcgccgg ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt taatccagaa
     8521 atcaatgcgg gaatactttc aggtatcaag accttccaga tgatttccca gtaacttgct
     8581 cccattgcat ctgctgcttc cagtacacca gggctgactt cacgaaaagc aatctctact
     8641 agacgggcat aaaatggtgc tgcggataaa acaagagctg gaatggctgc ttgcgcacct
     8701 agcatagtgc ctacgattgc tttagtaaat ggaatgatca aaaccatcag gatcataaat
     8761 ggcgttgacc ggaaaatatt actgatgatc gatacgattc cataaatgat tcgagcatac
     8821 ggtttgtttt tgcgtccaat agagtaaagg atcaatccca aaactaatcc gatgatcgtc
     8881 actaagacca ttgagattaa tgtcatatat agtgtatcaa ggatagcctg attcatcgtt
     8941 tccgggttga tttgtttaaa atctagatat gtttctgaaa agcttttatc cacgatggat
     9001 cacctcaatt cctactcctt gttctttgat gaacacttct gcttgatcta attctttggt
     9061 ttcaccagtc atcaagacag tcaatgaacc aaaggaacct tctttcactt gtttgatctt
     9121 tccatacaca acgttgacat cgatgttata gcgtcgaatc gcttgggaaa tgactggctc
     9181 gttggcattt ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat tttctttgat
     9241 gagttcagct aatagttctg tcgaatcagc ttctggttcg atgtcttgtt gtacaaagcg
     9301 tttggttact tcttttttcg gttgtctgaa gacagtcaat acgtctcctt cttctacgac
     9361 tttccccaac tccatcacag cgactttgtt gcagattttg cggatcacat tcatttcatg
     9421 tgtgatcaag acgatggtca aattcaattt cttattgatc gctaacaata agtctaatac
     9481 ttcttctgtt gtttgcgggt ccaatgcgct agtagcctca tcacaaagca ggatctcagg
     9541 atcatttgcc aatgctcgtg cgataccgac gcgttgtttc tgtccgccgg aaagttgtga
     9601 aggatatgct tgtccgcgtc cttctaaccc gactaaacgc aacagttctt ctgcccgttc
     9661 tttacgtttg ctctttggta cgccagctaa ttccagcggc agctggatat tttcaagtac
     9721 agttcttgac cataacaggt tgaaatgctg gaagatcata ccgattttct ttcggaatgt
     9781 acgtaattct ttgtttttta attttgtaat atcttgatcg tttacgatca cttcgccgct
     9841 cgtgggaagc tcaagtccat tcaggagacg gactaatgta ctttttcctg cgccagaata
     9901 gccgacgatt ccataaatat cccctttttc aacgtttagt gaaacgtcat tgacggcttg
     9961 gatgtttccg tttttcccat tgaatgtctt tttgacattc gttagttgga ttaatgacat
    10021 gccattcaca tctttctctt atgatttttc aaacaaaaaa gccttcgccc tcttgcctta
    10081 agcaaaagga cgaaagcttg aacttccgtg ttaccacctt gatttaccgt tacttcgcaa
    10141 taacagcctc ttccagtact tggacttagc ttctttccgt atactgtcgc gctgtaacgg
    10201 gcactcccgt agtaagtttg cttacgctcg cttactctgc tctaagacca tcttccatct
    10261 cattttcgtt acccattttc agcaaacagg gctctcttca aacgtccgtg acatgtactc
    10321 ttcttttcaa agctttctcg tttcatatag gattgcttat gattatagca actggaagaa
    10381 tcagtgtcaa cttttttcaa gtgattttct gattttataa ttcgtcaaat tcttttggat
    10441 caacatcttt gaaacttaag atccacgcat tcatttcatc tgaatcattc aagatattga
    10501 tatcttcacc gattttatca ttaacagaag aaactgtacc cgttagcggg ctggcaaatt
    10561 cattgaccgc tttttccgct tcaacttcga tcagaggttc atcttttttt agcgattgtc
    10621 ctgcttttgg cagattggca aacgtgatgt ctccaagttc ttcttgcgct tcatttgtca
    10681 aaccaacaca gtattctttt ccattaaata agatccaaag gttgtccttt ttcttcaaat
    10741 gcttgcctgc cattttatat cagttctcct tcataaattt tttcattgaa tccaattgca
    10801 acaagctggt cctctcgtaa gatcagtggt cgcttgatca gcataccatc tgtcgataag
    10861 acttggctcg cttttgaagc cgtaaactcg cctacttgat ctttcagtcc aagttcccga
    10921 tatttcatcc cgcttgtgtt gaacaggcga cggattggta cttgactcgt ttctagccaa
    10981 gtctcaatcg ttgaggcctt cggtacttct ttgatcatat caacggtttg ataactaacg
    11041 ccatgctggt ctaaccacgc tttggctttt ttgcatgttg aacatttcgg gtaccagtaa
    11101 aaggtgtaca attttcttac ctccttgaag atcttgcagg tgcttcattt tggtatctca
    11161 tcgataatat caacccgata ccgatcatgt tccctaaaat cgaggatcct ccctgactaa
    11221 tgaaaggcaa tgggatccct gttaaaggaa gtaatccgat atttgctcca atattctcaa
    11281 agacatggaa cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa aattcattat
    11341 tcgtatcgaa acacacccga atcatacgat agattaaaat aaaatacaag aaaatgacga
    11401 atgcaccacc gataaagccg aagttttcac cgataacggt aaaaatcata tcggactcac
    11461 gaacaggcac atagatattg cttacgttga atcctttacc aaacaagcca ccggtaccaa
    11521 ttgccaaaag tccttgggcc ggctgataac tcattcctga cgtatcatgg aaagggtcca
    11581 gccatgaatc aatacgtgca aactgataag atttgaatcc aactttataa agcaagtctc
    11641 ttccgccttc agtcgtaacc aagaagatcg ttcctgctcc aacgactact gccaagatta
    11701 ctacaggtgc gatgatgcgc caagagattc cagacatcag gaaaatcccg ccaaagatcg
    11761 caagaaagac aagcatcgtt ccaaagtcat cttgggccat gatcaatgct aaaaccggta
    11821 ttgtcacgat caagagcttc ccaatcagcc aaaagtccga tttcatcgtc cgttctttta
    11881 cttgcgtatt atgtttagtt acgatcagtg ccatcatcaa gatgtacgca atcttcatca
    11941 actctgctgg ctggaaagta aacgcaccga atcgaaacca gtttttagac cctgttgaag
    12001 cagctaaatt ctgatcatag aatttgagca agagtcccat taccactaac cccaaaccat
    12061 aaaggtaagg agtcagtttc cagatccatt tggaattcaa atgcatgatg acaacgattg
    12121 ctgccgctcc aactaaatac cagacaactt gcatccctac gcctttcaag actgctggat
    12181 tgttcggatc atggctaagt gctacatata atgagagcag accaatcaaa cataacagaa
    12241 agactggtaa aatcacgcca taatcaatac gattatcgtt cgaagctttt aatttttttt
    12301 ccatgtttca tcccttttct taaaaaccat actgctgatt attatagcgg tccagttaga
    12361 taatggaaag tctttccata gacatttggg aaaaattaac tttccgtttg tgcagttgct
    12421 tgagcaaatg cagtacaaaa gcctactgct tatctttagt gtatcataga actttttttg
    12481 atacatgcaa ttatctacca gctgagattc tcctcgctgt gagaagtttc tgtgctttca
    12541 ttgtatcgaa ggatctcttc ggcaatctct agcagctgat cagaaaccag attggttcgt
    12601 tggatgaaat aaaaatatcg atggaaattc aacggagtca ttggcacatt ttctggaaca
    12661 gagcgcttag acaaaatcga gcgaccgatt ccttccttca gcaacgcaac aattacatcg
    12721 ttacttttca cgatcatctt cttatcttgg atattcatct cttcaaaata acgctgcgtg
    12781 taatgaaaaa caccagagtt tgcttctctt acaagccaca atttgctttt tgggtcccca
    12841 gcgatgacta gctggtcttc catcaaagag tatctttgta cgccggaagt ctctaatggc
    12901 ttttcgataa aaccaaaatc ggattcatgt ttcgctactt gttctaatga ttcatgagaa
    12961 ttcgtgattt cgatctcaaa atgcacttta ggaaaccttt tgatcagatg tttcatcaac
    13021 tgaggcaaaa gatagacagc aaaggtgtgg gaagcaacga tccgacatac ttcttcttca
    13081 ttttgttcat tgagcaattt ttgctgagtt tcttcccatt cttctaacaa aaccagtgtc
    13141 cgttcatata ataagtcggc ttgcttagtt ggaagcatct cttttcttcc gttacgttga
    13201 aaaagatcga tattcaattc ccgctccaac tgtttgattt gattcgagac tgctggttga
    13261 gataaaaaca gtacttctgc tgctctggaa aaatttcttg tttcatatac tgctcgaaaa
    13321 gtcgttaata acttaaacat ggcaccactt ccataacttt ttatgataat tactataaaa
    13381 actatttgat ttaaatatac aaaaaaccat ctataataga aatgtacaac aaaacagttg
    13441 ctgatccaac tagtaactta gatgaagaca atcagctgtc tgcttgctcg taatcataca
    13501 ttcctattca ttttacaatg tattcgttca tcttggtact aaaatctatt aaataaaaag
    13561 gagaatatta tatatgaaga actttttgaa aattattccg atcccgatct gcgggttgat
    13621 ccttgggtta gcttcccttg gaaatctgtt taaagactat caccatgttg ctttaggcaa
    13681 tctaactggc ggtatctcta tgattcttat gatattcatt tttgttaaat tgtttatctt
    13741 attcgaacac acgaaacaaa cgttgcatga tccaatcatc gcctccgttt caccaacttt
    13801 tacgatgtca ctcatggtta tttgtactta ttttgtttca tatgaaagta ttgcaccaat
    13861 tgtcaaattc atttggctgt gcgctgttat cttccaagtg atccttgtct tttatttcaa
    13921 ttatcaccat gttatcaaag ctgatctttc aattgaagca atttatccaa gctggtttat
    13981 catttacgtt ggatttgggg tcatcactgt aactgccggg aacttcttcc cacttattgg
    14041 aaaaattttc ttttggattt cattgatctg ctatgcgttc ttattgccga tcatcatcaa
    14101 acgtgtcttt ttcgtaagaa atatggctca tccgacacgt ccgctgatta cgatcgttgc
    14161 agcacctggt tctctttgcc taacaggtta tcttaaaaac tttgctgaac ctaattttta
    14221 tcttgtcgta gcattgttcg ctttatctca agtattgtat ttcgttgtgc tgtctatgct
    14281 tccacgtttg ttcaagcttc gtttttatcc aagctatgca gcctttacat tcccgcttgt
    14341 tatctctgca acagctatat ttactactgt tcattacttc aacagtttag gtatctatag
    14401 tagcgtattg gatttcttaa aagttgcgga atgtctgttt gcaagtgtag ttgtcttcta
    14461 cgttcttgct cattacgtac gtttcttaat taaagaacat agcaaacatg gaaaatttgt
    14521 ttctaaaaaa atcgcttaat aatcgattca ataggttgtg aaaaagctgt tctgcatcaa
    14581 gtaataagaa cggccaaaca aaaacagctg ttcatgtttt tgtactggtt gggcttatta
    14641 ccgcagatgc aagcttttga acaccgttta ccaagaggtc gtaaaagaag tggtcagctt
    14701 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    14761 tttccgtaga agctacttct ggaacaccgt ttattcgaaa caagaggtcg tacaagactt
    14821 ttgtcacgac ctcttgttct tttgtcaaaa tactggttta gtgctactat gttcgagtat
    14881 gcttttagta tttattgacg aaaggaaccg atcaaatgaa atcattgtca catccaataa
    14941 aatctgtaaa ggattcagcg gtccgcgaaa tcttacccgg tttactgctt tcttttgcca
    15001 tcgctgcctt cagcaggctt cttgctattt atctgccaca gttaggtgga gcgactattg
    15061 ctattttatt gggtatttta ttaggaaata ctttttttaa acagcctgtt ctagcaatcg
    15121 gcacaaaatt tgctgaaagc aaactattag aattctctgt ttttttacta ggtgcaactg
    15181 tcacagctca aacgattgct caaattggtg taaaaggaat tgtttttgtg ttactgcaaa
    15241 tgtcagtcac tatcatcggt gcttacttga ttggtaaaaa actgcaattc agcgatactt
    15301 cttctttgat gatggctgga ggaaacgctg tttgtggttc ttctgctatt gcttcaatcg
    15361 ccccttccat ccatgcgaaa gaggaagaaa aaggacagat catcacacta gtcaatctac
    15421 tgggtactgt gatgatgttg actctccctt ttcttggatt acagttgttt ggtggaaatc
    15481 tcattgaaaa aagtgcactt ttgggtggta cactccaatc tgttggtcaa gtagtcgctg
    15541 gggcaagctt acttgacaat tctgttgtcg aattcgccat gctatttaag attctacgta
    15601 ttatgatgct cgttgttgtt gtctatctgt ttgaaaaatt catgcacaag aaacaaccag
    15661 ctctgtttga aacgccaact gatacgcaga aaaaacgaaa attaccgctt ccttggtatg
    15721 tattagggtt tgtgatcttt tgtttggtca acagcttgtt ttctttacct gtagttattg
    15781 accaatcggc acattttatc agcagctggt ttgagatcac tgctttagcg gcaattggtc
    15841 tgcgtttaga ctttgcaaaa ttcatgaaag aaggtacacg cttcttatct tatggattag
    15901 ctgtcgggct tctccaaaca gtgatggctt tactatttat ttggctgatc aaagtttaga
    15961 atgaacatga aaaaacagtt gtgattctcg catggaacaa tgtccgaatc acaactgttt
    16021 ttaattttgc agatatcctt ctaacaacaa caattggcgt ttgatttcaa ccccatctat
    16081 tgcgtttccc atatatcctg tcagttttcc attttgcccg atcactcgat gacaaggaat
    16141 aacgattggc aatggattgg ttcgatttgc ctgcccaatc gctcgcacag ctttcggact
    16201 atcaacagca acggctactt ctttatacgt tcggatttct ccataaggaa tggtcgacaa
    16261 agctttccag acattttgct gaaaaggcgt acctctcttt atagataaag gaacatcaaa
    16321 ttcttttctt tttcccgcaa aatattcttc aaactgttct tgtgtgcgcg ttaaaattcg
    16381 ctgattcgct gattcgcttt ctgaaattgg ctgaaaagat acagcagtca atccggattc
    16441 atctgcatct aaccataaaa agccgatagg tgtgtgtatt ttcattttgt ttgctcctag
    16501 tcaatagact ggaacttcct cctttttctc tatccaaaac tacttgccaa ctaataaaaa
    16561 ttgttcttaa aagtttgata aaccgaggaa acatggtaaa ctaaatacgt tagtttacaa
    16621 aaagaatacc tgttctttta cgtaaaatca aattttaaag gtgttattta aggtttatta
    16681 aattttgaat gtaaaccata tataaacata cgattgaaag gatagaaacg atgaaagtat
    16741 caattgttgt cccatgtttt aatgaagaaa aaactgttcc tctttttttt gatgaggttg
    16801 aaaaattcaa aggtgcccat gagttcgaat atttgtttat tgacgacgga tcaaaagacg
    16861 gtacgttagc agcaatcaag aagctagcct cagcttatcc ttatgtccgc tttgtctcat
    16921 tttccagaaa ttttgggaaa gaagcggctc tgtacgctgg attgcaagct tctacaggag
    16981 atttagtgac cgtgatggat gttgatctgc aagatccgcc cgaactgctt cctgagatga
    17041 tcaaccggat cactacttct gatatcgatt gtgtcggcac ccggcgttcg actcgtgaag
    17101 gcgagcctgc gatacgcagt ttctttgcga aaaaattcta ccagttgatc aataaaatca
    17161 gcgatacaga aatggtcgat ggcgctcgtg atttccgctt gatgactcgt caaatggtgg
    17221 atgccgtcct agaattaaca gaatacaacc gattttcaaa aggattattt agctgggtcg
    17281 gatttaaaac agaatatatt tcttatgaaa accgcgaacg tgtggctggc gaaacatctt
    17341 ggtccttctg gaaattattc aactactcga ttgatggtat cgtgaacttc tcggatgcac
    17401 cgttaacgat tgcttcattt atcggcgctt tgtcttgtgt cggttctggt attgcaattt
    17461 tgttcattat tttgcgggca ttgttgtttg gtgatcctac atcaggatgg ccttccatgg
    17521 tctcgatctt cctgttcatt ggtggaatcc aattgttatg tcttggaatc atcgggaaat
    17581 atattgggaa aatcttctta gaaacgaaac agcgtcctgt ttatatcata agagaaacag
    17641 aaaaagtgaa gccaaatgaa ttgaagcctc accaataata tacgtcaaat gaagtaaaca
    17701 tttttttaaa tgaaaaagga ctgtgacaaa agtcttgtca cagtcctttt ttccgaataa
    17761 atggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaag
    17821 caattttcgt aaattccttc ttatttcttg aagctgacca cttctatcac aacctctttc
    17881 atatcttatt acttcttcat tgctgctttt agtgctgctg ctaatgggct ttcttctggt
    17941 tcttctgctt gtgcatattt tttcagcaag cgtcgttctt catgtttggt cattttcttc
    18001 ttctgctctt ttttatcaag caatttttct gtcgttccat cgtatttaca acggaaataa
    18061 gcaccgtttt taccttcgat gatttccatt ttccggtgac attgcgggca tcgatgatta
    18121 gagacttttg gatcttttct tctgcggtag ttgcaatctg aattggtgca gacatagatt
    18181 ttgccgtcac gtgtgttttt ctcgcgcaga ttggccccac attctgggca tttcttttgg
    18241 gtaatcgaga aatcttgata ttcttgtttg cttgatttga tctcattcac caagcgtttt
    18301 gtttcttttt cgattccatt caagaaggtt cctgcttgtt tctttcctgc agcaatagct
    18361 tcaaggtctt tttcccagct ttctgtcaaa tctggtgtta ctaaagaggg atttaccaat
    18421 actaataatt gcttaccttt aggagatact tgcagtcctt gaggggtacg ttccatcagt
    18481 tcagatttga ttagtttctc gataatctct gcacgcgtag ctggggttcc cagactgtat
    18541 ttttccattt gaccaagcaa gcttgcttct gacaatggtt taggcggtgc agatagttct
    18601 tttttgatgg cgaaatcggg attgattctc attccttttt cccatttgac tgtttccgtc
    18661 tcagtttcag tttcgccttt ccatcctgct tggacgacac gattttgccg aaaaatgaat
    18721 cgttctttac caaattcaac cgtaactttt gtttggctga ctttatgggg ttgagcaaac
    18781 agtcctaaaa aacgagagac aatcatttga tagatttttc gctcttcatt acttaatttt
    18841 tcataacgtg ctctgttttc agttggcaaa agtgcatggt gatcggtcac tttactgtct
    18901 tggaatactt ttgtctgttg gacgatggct ccgtttttca aatactgctt gacctcagga
    18961 gcaaagtcgc tgactgcttg caatcgttct ttcatcgtac ttttcatgtc atttgtcaga
    19021 tatttgctgt ctgtacgcgg ataagtaacg atcttatggg tctcatacaa actttgtaca
    19081 agacttaatg ttttctttgc agaatagcca tagcgttgat tggcttctct ttggatctct
    19141 gttaaatcat aaggcaatgg cgcagattcc gtttttaatt tttcttggac ttctgtgaca
    19201 gttcctttgc ttgtactgat caattgaaca agtttttgtg cttcttcttg tgtttttaaa
    19261 gcaaattgat ttttttgaag cattttggct tgttcttttc cagcttgcaa cgtgatcgtg
    19321 aaatacgttt gtggacggaa ctgctcgatt ttcttttctt ggtctctcac taatgctagt
    19381 gtcggtgtct gcacacggcc tgctgataaa ttatccgcgt acttcacagt taaggctctg
    19441 gtcacattca atcctaccag ccagtccgct tttgcccggg caagagcaga ttcatacaat
    19501 gcatcatatt ctttcgcagg tcttagttgc ttgaatccag ttttgattgc tttatctgtt
    19561 tgagaagaga tccataaccg cttcactggt ttattgaaat gcacatactc taagatccag
    19621 cgagcaacaa gttccccttc tcttccggca tctgtcgcaa tgactgcttc tgagacatct
    19681 tttcgctgag ccagctgctt gattgctttt aattgatgtc ctgttttagg aagtggtttg
    19741 attcctagtt ttttagggat cattggcaac gtgtccattt gccagctctg ccattcctta
    19801 ttcagatctt ccggcatttt caatcctaat aaatgaccaa gggcccaagt aacgatcacc
    19861 ttgggtcctt cataataatt tttgtgcttt tgattagcac caagcacctt gcttaggtct
    19921 ttcgcaacgc ttggtttttc cgctagaatt agttgtttca taaaattcct ttctgctttc
    19981 tactgtgatg ggctgatgat ttttcaacaa cgcaagtccc ttgtattgct gtaaatcatc
    20041 gtcacactct tcacaccagc gctcatctcc gtcattccaa aaggcgctga gaaagttact
    20101 ttttgcattg atatattcat aattttgatg gatttcgttc cattgttttt cgtacgcttg
    20161 tcgtgcttct tcgaaagact cgaatgtttc ttcttcttca atatcttctt cccagtcttc
    20221 aaaaaaccac caaggctcat tgtccccgaa catggtaatt acttgataca tactcgtgct
    20281 ccttctttca gcttttctag actcatctta gtcacttttc aagaaagttg caagaatttt
    20341 ctcttataaa agggtcattt aatgaacatc ttaaaattgt tacaaatgaa tctgattgtt
    20401 taaggcaaag tagcgataaa gttattgatc atccaaaaat agctgtcttc tggatcgatt
    20461 gttggttctt ttgtaaagta tcctaaaaaa cgtaaggaaa gataaccgtg gatcatacta
    20521 cgaagttccc tgcttcgatg aactttttct tctttcgtta attgaaaagc ctcaaatatc
    20581 tgcaaaatga tctgattcgt ttcatggatt ccatctaata aaacagcttc attggtatgc
    20641 ggtacactga tcagtaactc atacactgct tgatttgaaa aagcaaagga tcgataagtt
    20701 tctgcataga tctgcagtgc ttcctgacca ctttttccca caagagcccg tcgcagctgg
    20761 tcattaagtt cctgcagaag tttcgctgtt agcgctgttt tgacatcacg aatatttttg
    20821 aaatggttat acagcgaggg agccttgata tccaagcgtt tggctaaatg ctgaaaggtt
    20881 atttgctgaa catccatctc ttctgctagt tctcgaaagc agtcgatgat tttctcttga
    20941 gacagatttc tttttttcat ttccatgttt cctctcataa aataaatcct tcactctttc
    21001 agtttaccac gaaaaagaaa gagaaaaaag agactttcat tgataaacta catattgtag
    21061 tttataataa agagctgtag atgaaaattt tcagaaagaa ggagtctaat tggctgcaag
    21121 aataaaggaa aaattattta ggaaaagaga aacttgggaa caaaacttgt ttgtcttatg
    21181 gtttggaaca tttatggctg gtatcggatt tagtttagtc atgcctttta tgtcacttta
    21241 catagatacg ttaggtaatt atagtacgtc acaattgaac ttttggagcg gattgacttt
    21301 ttcatctact ttcttggtga ctacgcttat ctctccttgg tggggaaaat tagccgacca
    21361 aaaaggaaga aaattgatgc tgttgcgtgc ttcattagga atggcaatcg tcatttcttt
    21421 gatgggttgc gtgactagtg tgtatcaatt agtcgcattg cgtctgcttc aagggatttt
    21481 ttctggttat atcagtaatg ccactgccct tgtcgcaact gggacgccaa aagaaaaaag
    21541 tggtcaagtt cttggaacct tagcaactgg ttctgttaca ggaacattac ttggaccgct
    21601 tcttggcggg atctctgctt cagcgtttgg ctatcgccct acctttttca ttacagggat
    21661 catcttattt ttcgtcttct tgttgagtct gtttttcgtc catgaaaaat ttgtcccagt
    21721 gaaaaaagaa aacatggttt ctgccaaaca gattttcaaa gacttgaaat atccacacgt
    21781 cgtcatcggt atgttcgtca caacgatgat catccaagca tccaacaatt ccattagtcc
    21841 aatcatcagt ctgtacattc gccaattgct gcatggacat gggaatgtta cgttgatcag
    21901 cggaatcatt gcttcgatac ctgggattgc cactttgata gccgctccaa gatttggtcg
    21961 cttaggtgac aagattggga gtgaaagaat acttgcgatt ggtttgggtt ttgcgattct
    22021 ggtttatatc ccaatggcat tcgtcacgaa tgtttggcaa ttagctgcct tacgtttttt
    22081 gattggtatc tctgatgctt gtctgctccc tgctgtccaa gcattgatca caaaatattc
    22141 gcctcatcaa gctgctggac ggatcttcag ttataaccaa tctttccaag caactggtaa
    22201 cgtagtaggg cctatgatcg gctccagtgt ttccagtgca tttggttatc gtggcgtctt
    22261 tctttcgact tctcttcttg ttttggtcaa cttcttgctt gtacgacaca atacacaaga
    22321 gatccatcag caagaaactg cagaagaaaa agcttcagga gtctccatag cttcagggca
    22381 ctctccttct catagacaat aagcgtaaat gaaaaacact gcttgaacag ttgtcacaga
    22441 ctgtccttgc agtgtttttg tttaggcctg ttttttcaag aactcagata agaggaataa
    22501 tcccttttcc aaggtttctt ttttacaaca gtaacctagt cgggcatgct tcggtaaatc
    22561 aaaggcactt cctggtacta atagtactcc tgtttcttct aataaattga tacaaaatgt
    22621 ctggtcatct tcttcgatgg ctaatttaat gaaagaagta gagacgtaat tgggagcgac
    22681 aagttcaact ttcggttcat tggctaccca ttgttttaaa atcacaaggt tctctgtaat
    22741 gatctttttg tttcgcgcca atattttttc tttattcttc aatgcatgta cagcaagatc
    22801 atctgacagg actccgccgc aaatcatcgt gtagtctcga tactttcgaa aaacttctgc
    22861 caactcaggt ccagtagctg tccagccaat acgaatacct ggaatggagt aggttttgga
    22921 tagggaattg gttgctatac ctttttcata gacatctacg atcgacaaga attcaccttt
    22981 atccgttaaa ggcgcatata cttcatctat caatacatat gcatctactg tacgagcaat
    23041 ttctgcgatt ttttctaaag ttttccgatc tagcaatgtg cctgtaggat tgtttgcact
    23101 attcaaacag atgatttttg tttcaggttt gactaaggct tctagctgtt ctaaatcaaa
    23161 ctgccaatct tcttcttcct tcaaatgaac aaagtcaaca gcggcaccta aagatttggg
    23221 gatgtcatat aattgctgat aagtaggaag catcgaaaca acatggtctc ctgcttccac
    23281 taacgcgtat aaagctaaaa gattagcacc agtcgctcca ttcgtttgta agatgttttc
    23341 cggatcgacg gtttgataaa gagaagcgac ttcttgtttg aaaacatctg atccttcgat
    23401 ccagccatag tccaatggtt cgtttttgcg tttttcgaaa aatttactta cgctagtccc
    23461 atctaagcca acgagttcgt ctaatgtcaa tgcttcgatc gagctttgtg caatatcata
    23521 taccgctttc ttttcccatt tatttagcca ttcttctaca ccgaaatttg cgattttcat
    23581 gtttctttcc cccttatatc ttgatccagc ctacagcaag caacccgatt gaaagcagtg
    23641 cggccacaca aatcagccca atcattactt tttcttttcc cgtaatttga tgtttggttt
    23701 cacgacaagc gatccagtag aagcaaaaac caggaagata ggcaatacta cataaaagga
    23761 cataagaaaa tcctgctaaa atgatgccca tcaattcaaa aactacagca atcccaccga
    23821 tcactacttg ttttttgatt ccttctttta aagaaaactg gacttgatag atcccgacaa
    23881 gtagataaca aatcaaaatt gccgctgtac ataaagaata ggcaaattga taggcataat
    23941 cagtaactaa gaaagtcaat aagaatagat tagtcaagat tgcagtaaca actaacgaat
    24001 aagttggggc atttttttta ttcgtttttc cccaaccttt cggcaacaga ccgtctcgag
    24061 ccatcaacat cgttgtttct gctgggagca tcgtccaaga caaccaagaa ccaatgatcg
    24121 aaataatcaa gccgatattg atcagcgctg ctccccaatt tcccacgatt ccttttaaaa
    24181 tatttgccat agcaggttgt gaaatagttg ctaactcttc ttgagataag acgccgtatg
    24241 gcaagatcga agcaaggata tagataaaca ataaacaaag cagaccaatg atcgatgctt
    24301 gctgggcttc tgaacgtttc ttcgcgcggt tggctaaaac acttgcgcct tcgatcccta
    24361 caaagaccca catcataacc atcaaacaac ctttaatctg caaccagatc gttccttgct
    24421 gccctgttcc attcactaga ttatcagata cgttacccca aaaatcagca gtgaatatat
    24481 tcactttaaa tgcgataaat acaaatatta aaaataggaa taatggcact aatttacaga
    24541 tcgtcacgat tgtattgatg acgctcgctc cttcaactcc attgtttact aataaagtca
    24601 gaccccaaat aaaaatactt gcaaataaaa tagaaggaac attttgtcca cctttgaata
    24661 tcgggaaaaa ttctcccatg gcgctcatca gcatcgttgc aaaagcaaca tttcccagcc
    24721 acgcagacaa ccagtaaccc caaccactga tgaagccacc taatttacca aatgaagctt
    24781 ccgcgtaacc gaaaatacca ccatctagat ctggacgctt ttctcctaaa tgattcagcg
    24841 ataataccaa agctaaaata ccaatcccaa cgattatcca tgaaaggatc gctggccctg
    24901 gagctgcaga actagccaga tcacttgtga tcccaaacac accgcttccg attgacgaac
    24961 tgacaaccag agcaaccagt ccccatttgc taatacccgt cgatttcttt tccatttcca
    25021 tcaccctcac ttataaaatt gaacaaatca cttgcaaaag attcccttct attttgcaac
    25081 tgctcttgct taacaaaaag caactttatt catattattg gatattatga actttttaca
    25141 caagtatttc aagtataaaa tgaataaaaa ttaattttat tgcaaaaaaa cagctatgct
    25201 atccaaaaga aaaggtgttt cttttgttag ccatagctgt tttgattcat ttaatatcgc
    25261 ttaaacgtat tgtaaaactt ttgtttcttg atatgcgtgg tcgatcaaac caccacccag
    25321 acattccatt ccatcataaa atacaactgc ttgtccagga gtgattgcac gtacaggttc
    25381 gtcgaatacg acttctgcac gattatcatc caataagcga actgtcacag gaacgtcttg
    25441 ctgacgataa cggaatttcg ctgtacactt gaattctttt ggcattggtt cattcgtcgt
    25501 aaagtggatt tcacttgcat ccaagcttgt ggcatacaat gcaggatgat ggaacccttg
    25561 ccccacatat aatgtgtttg tcgctagatc ttttcctaca acaaaccagg gttcttgcga
    25621 atctcctccg ccaccgatcc ctagtccttg acgttgacca atcgtataat atatcaagcc
    25681 agcatgttgt cctttgactt caccatcaag agtgaccata tttccttttt ttgctggcag
    25741 atagttgctc aaaaattgtt tgaagttctt ttcaccaata aagcagattc ccgttgaatc
    25801 ttttttctta gcagtcgcaa ggcctgcgcg ttcagcaatt gcccgtactt ctgatttttc
    25861 catgccgcct agagggaaca tcgtttttga aagctgttct tgtgataatt ggctaaggaa
    25921 gtaagtttga tctttattat tgtctactcc acgaagcatg tggaccacgc cgttttcatc
    25981 gcgagtgact tgcgcataat gtccagtagc tacataatcg gcgccaagat ccattgcata
    26041 atccaagaat gctttgaatt tgatttcttt gttgcacatc acgtctgggt taggtgtacg
    26101 tcctgcgcgg tattcggcta ggaaatattc aaatacacga tcccaatatt ctttttcaaa
    26161 attcacagag taataaggaa tcccgatttg agcagctact ttcgctacat ccttataatc
    26221 ttccgtggcc gtgcatacac cgttttcgtc cgtgtcatcc caatttttca tgaatattcc
    26281 gatcacgtcg tatccttgtt cctttaacaa aagtgctgtt acagatgagt cgacaccgcc
    26341 gctcatccct acgacaacgc gtgttttgct gttgtctgtc atgatatcac catcatttca
    26401 attagattct gaaacatcta cgagttgaag gtcgcaactt ttcagtattt tacattatac
    26461 gtcatatttc tgaaaatttc atcccttaat tatcaagtcg taaaaatact gttctactgg
    26521 acctattcag tacacaaaca tgatagtcaa ccaaaaaatc actcatcttt tgagcattcg
    26581 atataaaaaa ttggcatact cataaaaagg gccagccttt cggcaataag gaataatttt
    26641 ataaattgtg aaaaactatt tttcaaaatt gcttcttatc gcttaaagct gcacgtctca
    26701 tttacaacct tttcttttta gatgttatga gttgctcaga taagtgattt tgtttactct
    26761 aagaaaaaag ggaggcaaaa gatacatgaa ttactttatc agtgatatgc actttttcca
    26821 tgaacgctta ttaggaaaaa acgactttgc tccgcggctc tttgcaaatg tggatgaaat
    26881 gaaccaacag ttgatcacta gctggaatgc agtaataaaa gaaaccgacc atgtttatca
    26941 tctaggcgac cttgctatgc atccgcaata tgaaaaagca aatgcagaaa ttttggaact
    27001 ggtcactcaa ctgcaaggaa caatccactt gatcaaaggg aaccatgaca gtcgcgcttt
    27061 tttccgttat ttagaaaaaa atgatcctag gcttactgac ggaagtccca aattttattt
    27121 ttatgatgta ggtgccatcg ttaaattcga ccatcaacaa tattacttga ctcattatcc
    27181 gatgttgctc gggcctaacg acactatccg caatcttcat ggacatatcc atcattacag
    27241 cgtacctatc gagaacgatg tcaatgtagg cgtagacgca ccggaaagag aactcttaaa
    27301 ggaaaaacta ccatttggca ctccgttatc tgagcaacaa atcaaagaaa tctatgaagc
    27361 aaagaggaag tacgataggt cgtgaaaaaa ctgccctata tcaagtatta aacaaataaa
    27421 gataattact catatttttc atttgagtag gcttattgcc acaggaacat taggatcaga
    27481 aatggagttg taacaagact tctgtcacaa ctccattcca tactattcgt ctttttgctc
    27541 atggtgttct tttaccagtt ttttctgtaa ttctttatca taatttcggg cagctgacgt
    27601 taaaaataaa acgattccgc ataataaaat cccgattccg gaaaacaaga acattttttc
    27661 tacccctaaa cgatcagcta atggtcctgc aaataataat ccaattggac ctgccaggtt
    27721 catcaaagag ttgaagttcc ctaagacacg acctaaaaca ttcgattcat aactctgctg
    27781 gatcattgcc atcagcagtg tattgaaata tggcgttgca caacctgctc ctgcattcaa
    27841 gataagaaag tacaagaacc cttgactatc gtttggaacc aatccgcttg caccgatcgt
    27901 caggcccata atgatgtagg ctataataat aggtttcatt cggtctttcc atgttcctat
    27961 aaaactgatc agtgcaccgc ctaagagcat tccgactgcg tagactactt ccactgctcc
    28021 cgcctgtcca acggaaccac caaagtaatc tagcgtcatc aatggataca gactgattgc
    28081 tggcatgaac aacagcatca caaatgcacc attgatcgtt atgtaccata accctttatt
    28141 atccatcaat tgctgcagcc caaattttgc atctttaaaa atagtaagcg gaacttccaa
    28201 tatttcagga gaaacttttg ggatcttcac aaaaatcaat aagccgacac caaaaactgc
    28261 ccccaataca tctaataaaa taaggtaatt gacaggaacg actgaaaaca aagccgcacc
    28321 tagagctggc gcaatgatga aattcgctga ttggaccatt cctaactgtc cattcgtcct
    28381 tgttatgtga gaagatggaa cgattgtcgg cataatcgac tggatcgttg gcatttggaa
    28441 tgtttgtgcg accgaacgca taaacaaaga cacaaaaact agccaaatcg ggaaggattc
    28501 tgaaatcgtt ccgactactg ctaaaataag cgcgaaaatc gctacaataa tatccgtgac
    28561 aatcaaaaga gcttttttat cccagcgatc cactagcggt cctacaaatg ggcttagtaa
    28621 aaccatggga atcattccta gtaaagtagc aaaactcaaa attgtcgccg aaccggtttc
    28681 tcttgtcaaa taccagatga tcgcatattg gacgaccata ctagtgattc ctgacaaaaa
    28741 ctgtcccgat aaaaagaaca aataatttct cttccaattc gttaaaacga ccgactgttc
    28801 attttccata aacatccctc ttttcttttt cattttgtat agcgtttgca caacaatcat
    28861 tatacgggat tcttttttca aaaaatacct ttttccctaa ataacaatcg tttcaattta
    28921 aatttcttta cttagaaaca ataaatcagc aaaatattaa aaataacaac atgtgttaaa
    28981 tttaaaaata aaaaagacaa ctaatttgcg tcgtccttac aaagttagtc atctaacaat
    29041 aggccgtttc aaaactagtt gtctttttat atccacagat cattcgactt tttcgaataa
    29101 cccacggcta atcatcccat ccatcagaaa ataaaattta tcttggctca ttttctgttt
    29161 tggatctttg aaaatattga ctgctttcaa tccatttgct gtagtgatcg acattttcaa
    29221 tgtttgaata tcttttgata cagtgatttt taatttgaat ccttctttgc tttgcggcgt
    29281 ggcatctaat ggtcggatca attgataatt gcctccgttt gtttctgtaa atccattatc
    29341 gcgcaatgta tatctttttg catcaggatg caaacgatag ctaattggtg cgcccagtac
    29401 cgttgctgtt gtttcaaatg ccatgatttt cacctcgata aaattagttt ctctttatta
    29461 taaaagttta tcctgatttt tactaggttt ttcagttaag ttccctaaaa aatgaaggcg
    29521 ttctctcaga catctgttca tataagaatg acttcactag ttattttttg acagcacgaa
    29581 ctaattgatc aaggaaatac tggatgtcgt cttcggtatt tcccaaacca aagctgatac
    29641 ggatggattc ttttagtatc ggtgtatctt ctccatacat tgcttctaat acgtgtgaag
    29701 gctcgatatt tcctgctgta caagctgaac cagtagaaat agcgacccca ttaagatcaa
    29761 gcttcatcag tagcaaatca tttgcttcgc caattaagcg aagattcaac acatgaggga
    29821 gtttattcgt taaatcacca tttacttgat aagggatttc gtttgcttct aatgtatcaa
    29881 gaatcaattg ctggaatcct ttatatttgt tgttacgtgc ttcacgttct tcaggtgaca
    29941 gcagttcaac tgcttttgcc ataccccaga tccctgctaa attttctgta cctgcacgcc
    30001 gcttttcttc ctgctccccg cctaaaagca atgcagggat atttactccg tctcgtttat
    30061 ataagaaacc gacgcctttt ggcccattga ttttatgagc agagatactt aataaatcga
    30121 ttcctaattc ttctggatgg atcgtttggt ttccgtacgc ttgtaccgca tctgtatgga
    30181 aataagctgg atgatccgct aaaagttcac cgatttcttt gattggcatc aaatttccga
    30241 tttcattgtt cccgtacatc acagagacca agatcgtatc ttctcgtaac gcttttttga
    30301 cctcttcgat cgacagattc cctttttcat ctacaggcag atatgtaacg tcaaacccct
    30361 ttgtctctaa atatttcatg gtattaagaa cagcaggatg ttcgatagct gtagtgatta
    30421 gatgtctgcc ttctttttga cgcgagaacg ctgtttctaa gatagctgta ttatcccctt
    30481 ctgttccccc actgttaaag atgatctcgt gaggttttgc ttgcagactg tctgcaatga
    30541 tttgtcttgc tgtttccaat ttttcatgcg cgtggcgacc aaatccatgt atgcttgaag
    30601 gatttccaaa cgtgtcttgc atgattgccg tcatcgcttc aattacttgt ggatgcatgg
    30661 gggtagttgc cgcatgatcc agataaatag ttttcacttc attcatccct tccaaattat
    30721 tgctaggcac attctagcat aaaatagcga acaaaccaaa gaagagctca tttttgtcta
    30781 gaggacgttt catgggcata gaaaaaagga gctgtgacaa aagtcttgtc gcagctcctt
    30841 gaagctgctc acttctgtca caacttcttt ccctcttttt tttgtttatt cgaattcttt
    30901 ttcttccaga cggaataacg gactcatcgg tgtattttca tggatgcgtt tgaccgcttc
    30961 acccattaat tctgaacatg taacgattgt taagttttct ggatgacgat tttcttctgt
    31021 gaaaacagaa tcagtgatac aaatatcttt gattggtgca ttgtctaaaa tacttttcgc
    31081 atgatcggac aataatccat gtgacgcaca ggcatatact tcttttgcgc cgttttcttt
    31141 taatacattt gctgctcgtg caagcgttgc tcctgtgttc aagatatcat ctaccatgat
    31201 acatgtcttt ccttgaacat caccgatcac ataaccaccc tcagttgaag catcatctgc
    31261 atgatctacg atggctaatg tgctgttcaa atattctgaa agactacgtg cacgctgaac
    31321 accgctgttt tttggcgaaa caactacgat gtcatctccc gtcagaccta attgacggta
    31381 ataatgagca aacaacggca tcgtaaataa attatctaca ggaatatcaa agaatccttg
    31441 tacttgaacc gtgtgcagat ctaaagtcag tactcgagtt gcccctgctt cttcgatcaa
    31501 gttagcgatc aatttcgcag tgatcggttc atgaggtttc gccgtgcggt cttgcctagc
    31561 atacccatag taaggcaata cgacattgat cgttttcgca cttgctcgct tcattgcatc
    31621 gatcatgatc agtagttcca tatagtaatc attgacaggt gcattcgttg attgaacgat
    31681 atacacatga tcccctcgaa tactttcttc gatattgatg gaaatctcgc catcactaaa
    31741 ttgcttcacg acgcttttac ctaattctgt tccgaacact ttcgcgattt tttcagctaa
    31801 tggacggttg ccattcaaac taaaaatctt caacgtgtca tcctgatact tattactcat
    31861 gtagtcctcc ccaaaacttc tctattcttc gatagtttaa catatttttc caagattttc
    31921 tgttaatttc agcaaattct taaggaagtg aaactgttcc gttactccaa ctggcaggaa
    31981 tgactttatg ataatcgccc cagttgtcgt gtactttgta ataacgttct cctgtttcag
    32041 tttcaaaata agcgtacgct gtcacccaat gattgccata tgtactgcca agtactttca
    32101 aaataccaat catcacaggt ttgccttcac gtatacgttt tgtagctcgc tgccatgaac
    32161 ctagaagagt tgctcgtgca cgctcatgat tgccattctt ctttaaaaaa ctagaaatcc
    32221 ctgtactaac ctgaaaagga atcgtaggaa gtcctagtgg ttggatctgg ctacgcaatg
    32281 ccttcgtcaa aaccaacgaa tcagccgtat ttttcttgcg aatctcattt ggaagcatgc
    32341 accccttacg atagtcttga taataggcaa ggagaactgc tgccgcgtaa gtaccacaaa
    32401 gatagcctga gggggtagcc catttcttat acctcaaaaa acgttcttct ggcaatccca
    32461 cccagcaatt tgcgagttcc tttcctgacc ttttttcttc gatcaaaacg ggaatatctt
    32521 cttttctgtt gatccatcta gataactcga ttggctcttc attgatcagc ttccattttt
    32581 tacaagaaag accctcttga taagtatatt ttgctggaga atattctacc ggatgtcctg
    32641 attgatcgat aatcacataa ctttgatctt caaaaaccag agctaaaaaa agcaatttac
    32701 tttttcttga ctgcagctgc tgagtgctgg ctaatttttt aattttttta tctactttga
    32761 aatataaagg tttatccact acttgattca gttgggatcg cctccttttt ttcattataa
    32821 gtgaaggatt gaaaaaaaca aatcagtttg attgttcgaa ttttcaattt tttatgttct
    32881 atttgttaat tccttcattt tgtaagataa tgggcacacg aaacaaaaaa ggagcgatcg
    32941 atgaatgaac aaagaacgat tgaaacaaga tttctatgaa gcagtcaatg aagaatggca
    33001 aaaaacagca aaaatccctg cagataaacc agctacaggc ggtttccaag atttagttga
    33061 cgggatcgac caacttttga tgggagaaac agacgatctg cttgcccatc cagaaaaagt
    33121 gtcttcagaa aaaatgaaac atttccttgc atattatcaa ctggcaaacg attatgaccg
    33181 aagaaatgag ttgcatgctc aaccgcttca acctataatc gaaaaagttg aatcattgca
    33241 gtctttcgac gaattgaata agcaatttcc agaatggctg ttggatacat taccattgcc
    33301 atttaatctg gatgtagatg cagatatgaa gaatgcgcaa acgaacgcct tctttgctta
    33361 tccgccttct ttattccttc cagataagac gtattatgca tcagaccatc ccaacggtgc
    33421 acaactaata ggcgttttct ttgatatggc tgttcagtta ttagagcttg ccggcaaaga
    33481 aaaaagtcag gcagaagcaa tcacggaaca agcagtgcaa tttgataaat tgattgctcc
    33541 tcatgtaaaa agtgcagaag aaaacgcaga ttacagcaaa atgtataatc cgcaatcttt
    33601 tgatacattt attaaataca cagataaact ggacttgaaa aaacttgtca cgggtttgat
    33661 tgggacaact ccagataaag tgatcgtaac tgatccagtg tacttcgatc atttgaatga
    33721 catcctgaca gaagctcatt ttccgttatt caaaagctgg atgatcatta aaacagtacg
    33781 ttctcttagt ggttatctat cagaagaatt ccgccaagtc agcggaatct attctcgcgt
    33841 attgtctgga acagacgaag caatgcctca gaaaaaagca gcgtattacc ttgcctctgg
    33901 acaattcgat caagtagttg gcgattacta cggaaaaaaa tattttggcg aagctgctaa
    33961 aaaagacgta gaacaaatgg tgaaaaaaat gatcgccgtt tataaaaaac gtctagaaga
    34021 aaatgactgg cttggggatg caacaaaaga aaaagccgtc ataaaattaa ataacttagg
    34081 tatccaagta ggctaccccg ataagatccc agcattattc gaccagttca aaacagtacc
    34141 tgcagatcag ggtggtactt tgctttcaaa tactcttgtt ttcagcaaac tcacattaaa
    34201 agatcgtttc agcaaatgga acaaaccagt ggatcgttca gaatgggaaa tgagtgctga
    34261 cacggtcaat gcttattatc atccatttcg gaatgtgatc gtctttccgg cagcgatcct
    34321 tcaagcacca ttttacagtc tagaacaatc aagctctgct aattatggtg gaatcggagc
    34381 tgttatcgct catgagattt cccatgcatt tgataataac ggcgctttat ttgacgaata
    34441 tggcaacttg aacaactggt ggacagaaga agatcttaca caatttcaaa acaaagcaca
    34501 agcaatgatc gaagaattcg atggtctgcc ttttgctgac ggaaaagtaa acggaaaatt
    34561 aaccgtttcg gaaaatattg ctgatgcagg cggtctaagc tgtgcattgg aagcagcaaa
    34621 aacggaatca gatctttcta tcgaggaatt ctttgtcaat tgggcaacga tttggcgtac
    34681 aaaagcaaaa aaagaatacc aacaactttt actgcaaatc gatgtccatg cccctgctaa
    34741 actccgagca aacgtgcaac caaaaaacct tgacgaattc tatgaaacat ttggtatcac
    34801 ggaacaagac gctatgtatc tagcgccaga aaaaagagtg cgaatctggt aagaggttgt
    34861 gccagaagtg gtcagcttca agaaataaga aggaatttgg gaaaatcgct tctcaaattt
    34921 ttgtgacatt tcggcttatt tttcgaagaa gttacttctg gaataccatg tattagaggt
    34981 tgtgaaaaag ctgcttagtt tcaagtagta agaaaaattt tgtgaaaatg gctttccaca
    35041 tttttgcata aatttgactt actaccgaga aactggcttt tgaacaccgt ttatcgagag
    35101 gttgtgaaat cagcgtcctg acctgagtat tagagaaaaa aacggaaaaa tagttcctca
    35161 tattttgccg tttgttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag
    35221 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa agcagcaagg
    35281 agaacctcat ctataaaaag atagaaggaa gtgagacatc tattggtctc atttccttct
    35341 atttttactt tcctcagtca attgttttac atcataaaag gagaaattcc acttattttt
    35401 ttcgctgttt catgacataa acaactacac cgattaacaa aattcctcca gctaatgcta
    35461 gcaacgggtt attaaattct cccgtttttg ggagtccttg agtgccattt ttttcacttt
    35521 cttttgctgt cctatccgga tgagaaggtt ttccatttgg ttttccattt ggtgattcat
    35581 ttcctggatt ttctctttct ggtttgtcat ttcccggggg cacatcggca gtttctcgca
    35641 cagtgatcgc tttttgctgg gtaacagatt ctgtatgagc agaccatttt tccccatcaa
    35701 atatttccga acgataagtg acttctagcg tatattctcc ttttgaaggt gctgaaaaaa
    35761 tagcggtatg attagaaaat tgagcacttg actgtgtttc aacgatttgg atcttttctg
    35821 ggaaataacg catatcttca aagacaggcg ttccttgatt tggtgtgaaa gttgacacaa
    35881 aaactgtaaa aggcttcagt tcaatgatat ttccttctgc attctccgcc acaccatttg
    35941 cttcttgtat aataatcgga ttattttgtt catttggttt tgtctgattt ggttcttgta
    36001 cgattgcata ctctggatat tccgaagacg gtgaggtttc tccgtcaaaa atagcaccta
    36061 cagataatgg aagaccatcc actctttcct tttctgtttg acgattcccc ggtttaatct
    36121 gcatcagtcc atgaacatgg atacttccat tttcatagcg cgtaacaggc tgctgacctt
    36181 ctttcgaaca tgtatttaga gactcgatcc aatcttttga tatggtatgt ccaagagtat
    36241 tttctgattg tttctctcgt tcattccaat agtagttcaa cgagtggtag atttgatctt
    36301 cgttttgatt caatgggcgg atctgctctc tcatctcaag attctctgtt cggaaagata
    36361 agataccaga agcatggtag tctgtgtttc ctgctgaaga ggtataaaga gccacattat
    36421 atgaaccgtt attaaaactt gtactgctat tgacgataat atctgttccg ctattggaat
    36481 cgattccttt cgcaagattt tcgaaagaca aactattgat caattcatga gggcccgtta
    36541 aagaagaacc gcccattttg aatccattac cgttaccttc tccctcaatt cctggtaccc
    36601 agccgtttcg ataagcaact gagtttttga tgattacttt tccgatcgaa cctgtttcat
    36661 ttttagcaaa aaggtcccaa ccgtcatctg cattatgata cgcgatacaa ccgtcaaata
    36721 catttccttc tcctattgtc agtttggccg caaatccatc ggcatcttca aatcctggat
    36781 cggcattttc aaaagatgta cagttcttga ctaagttatg cgcgggccat gcttcgaaag
    36841 gatcatttga gctaccgctg atttggattc ctgtatttcc attccgataa gcatgaatct
    36901 cttcgatcaa attatgattt ccagaaatct gtaatccttt ttgcatagct gcactatttg
    36961 taatgtcaaa ccctcgtaaa ccccagtagt gactgaacag tgtcatccca tttccagttc
    37021 ctttaaaatc aagtacagga cgcttgtttt ctggattttt ctctgcaatc aactgaatcg
    37081 ggttttctct tgtcccgtta acgccttttg gaatcgtcaa tcctctttcg aaggaataag
    37141 ttccaggcgc catgacgatt gtctgcccag catatgcgta tctcaatgct tgtgtcagag
    37201 ataatggatt ttgtctgctt ccgtttcctg catctgaccc ttctggtgtt acataaactg
    37261 tcgaatattt aggtttacgg tagtccactg tatgaagaat ctcttttgtt tcataactag
    37321 tcatctctac gtattcttct ggcggatagt ttttatctgg cgtaaaactg aaagtaaatt
    37381 cgttcgttcc ttccatcagt tttgtctgat aatcaaattc ccgattcgca gtgatagcca
    37441 agttttttgc gttcttaagc acctctcctg ttgctttatt tttaattgtc aataatccgt
    37501 cagcatttgc tctaaaggac atagtatgat cttggtttcc tgaatgatta ctagaggtca
    37561 cagtgtaaat cggatcaatc aagcgagtag gtctttcttg cccaggagga tctgtcgcag
    37621 gatctgagta agcaacttcg attccttcta ccttgatcgt catattcctt gctgcagcaa
    37681 atcctgcata aataaccgat tcatctgaag cgtataattt ttcccagtca tacatgatgt
    37741 cagaagccat ttcttctcct gtttcaggat cgtaataata tgcctctaaa ccagtattcg
    37801 tttttttcat ctctacgtcc agtttagtaa tcgagttcgt ggccggaaac agattcccat
    37861 gaccgtttcc gaatatatta taagaacctg tttctttttc caagaaccct gctgaagtct
    37921 ctaaaggtgt tgtttccact gagacactcc caggagctgg cgcagcttgc ggatcttcag
    37981 atgatattcc tgtgattgcc cgtgtcccca atccaagtcg catcgtatat ttgctgccgc
    38041 tcgtgttgac cacttcttgt ctatccgagt cccagacata ttcgatccga ctgcctagta
    38101 tggcaaatga atttgaataa aatgcctgcc caaacaaatg attttttggt actgcgtctc
    38161 ttaccattaa agcaaatcct tcttgggcat tcgtataggt ccaagattct atttcgatcg
    38221 tcgcccgcat cctaaagttg atgtctttgg ggattgcttg ataataatag gtcattccat
    38281 ctgatccgct tgactggaat tttccgccgt tattcgtgga agagagccgc acgccgtctt
    38341 ctaatggttc aatggcatac ctactagcac ttgtgctttc tccgaaaaca acccctttcc
    38401 actcttcatt tgcttcttca gattgctttt ttgtcttttc attttctgat tctttcatca
    38461 taggatcatt tccttcttcc gtatccatat cttcactagt tcttggtatt tcctccattg
    38521 ttgacatccc ttcaatgttc acgtgtgcat tttcttcgtt cacagactgt tgttcgttag
    38581 cagcaacttc aacgacagaa attaaaaccg gggtactgag cataaggaga ttcatcatta
    38641 gcgcactgta ttttatacgt ttttttaaca agtgtattcc tcccgtaaca aattagaaag
    38701 cgtttactta aagtattacc taatttattt ttactgtcaa taaaaaacaa aattataata
    38761 agtgtatttt ataaaaagaa aaaatataac ttaagttgtt gataataaag agatttatta
    38821 acgtaaaaag aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt
    38881 ttgttttatg ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt
    38941 tttataaaat ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa
    39001 cgggatcgaa acttgccacg gcatctttga aaatatggag tttgtatcct agattatacg
    39061 catcgactgc tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg
    39121 tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc
    39181 gtttgtctat ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag
    39241 agccgtacaa ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct
    39301 ccggatgaaa ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt
    39361 gatcaataaa cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc
    39421 cagtagtcag ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg
    39481 cgttcatctt agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga
    39541 tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt
    39601 gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac
    39661 agcggctcct tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat
    39721 tttatctggc gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa
    39781 attcgttgct tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata
    39841 tagaggcatt cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa
    39901 ataagctact ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc
    39961 agctgaacct gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt
    40021 aatagatgct aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa
    40081 tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc
    40141 tctcaaaatt tttacttctt cttccattgc gccgataata ccgattttca tctttttaat
    40201 tccttctttc tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat
    40261 aataagagat ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct
    40321 tgttttcgtg attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt
    40381 ttcttcttgc tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg
    40441 tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg
    40501 atccctctac tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc
    40561 atcacaaatc aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa
    40621 ctccagtact tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc
    40681 atgataaata tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg
    40741 gctcagcgac ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc
    40801 gggattttgt ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc
    40861 taaaggtttt cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat
    40921 cccgacaccg ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg
    40981 aacttcgtct acagcaacat caataatttt tccgctatag atttctttgc gactgattgt
    41041 tttttcttca aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac
    41101 tgcttttttt tcatttttca atttttctca aaaactcctt attagtttac catagataaa
    41161 ccttagattt tctctaaata tagaaaatcc ttctttagtc cgatactctt tatagtagtc
    41221 cgtatgctaa aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat
    41281 gacatggagg gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc
    41341 tatttctctt tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg
    41401 tagttttagt tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa
    41461 ttcctgcact gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg
    41521 aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa
    41581 tgaatatgca gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag
    41641 cagcgaaagc tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc
    41701 agtttcttta tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca
    41761 atgaccatga gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca
    41821 tcgaccagct gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag
    41881 atgatatcga cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg
    41941 aaccagaaca aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga
    42001 tgtctggctg tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca
    42061 gattcaacaa ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac
    42121 cagttaacga tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc
    42181 tgaccacttc acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt
    42241 taattgacaa actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg
    42301 atgctaatga tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg
    42361 aattctcaca atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt
    42421 ccttaactga actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag
    42481 aagggaacat cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag
    42541 caaaatatca aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa
    42601 aagacggtct gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact
    42661 actttgatgc actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa
    42721 caacgatcat tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc
    42781 agattgccaa tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc
    42841 gcttagctag acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa
    42901 atcaggcctt ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa
    42961 accaagtcgt gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc
    43021 aagtctctga aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag
    43081 ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca
    43141 ctcaaaatga tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag
    43201 aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac
    43261 tgttggattt aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca
    43321 gtttattaca actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt
    43381 ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat
    43441 aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat
    43501 acaagtcaag atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc
    43561 ttttggtttg ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata
    43621 ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa
    43681 gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc
    43741 gatttctgtc ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc
    43801 aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat
    43861 caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac
    43921 tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac
    43981 agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac
    44041 ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc
    44101 accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac
    44161 gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc
    44221 attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac
    44281 tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa
    44341 tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc
    44401 ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca
    44461 ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac
    44521 tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac
    44581 aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa
    44641 tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc
    44701 ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga
    44761 agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag
    44821 gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt
    44881 ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga
    44941 aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc
    45001 taagccccat aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt
    45061 acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga
    45121 catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga
    45181 gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt
    45241 tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat
    45301 aataccgtat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat
    45361 cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta
    45421 cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc
    45481 gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa
    45541 cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc
    45601 acttactaaa ccaatactac cccatgccaa catagggact aatgctatta aaatttccat
    45661 aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca
    45721 agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg
    45781 ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac
    45841 ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc
    45901 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
    45961 ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc
    46021 tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga
    46081 agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt
    46141 agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc
    46201 cttttagtcc atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat
    46261 acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact
    46321 tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca
    46381 ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg
    46441 ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt
    46501 tttcttcttc tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt
    46561 cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca
    46621 gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg
    46681 gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg
    46741 tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac
    46801 gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt
    46861 tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc
    46921 ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat
    46981 aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact
    47041 tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt
    47101 gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa
    47161 atgttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt
    47221 attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca
    47281 ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct
    47341 ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt
    47401 ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt
    47461 tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg
    47521 aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata
    47581 tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata
    47641 attggtctat tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca
    47701 ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata
    47761 aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg
    47821 tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt
    47881 gactagatcc tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat
    47941 tttctcaacg acttcataag cgagcatccg accatacact tcaagataga atcggtcatc
    48001 ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt
    48061 aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc
    48121 tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt
    48181 cggaatatag atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac
    48241 atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt
    48301 atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc
    48361 tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct
    48421 gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc
    48481 cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt
    48541 cattttcttc accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc
    48601 agcttatttt tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg
    48661 ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag
    48721 caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta
    48781 cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct
    48841 cacctgttgg aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat
    48901 aatctaaccc ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc
    48961 cgcttgttgc ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc
    49021 ctttataaaa tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt
    49081 taagcaattc actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt
    49141 tctctggcgg ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa
    49201 ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg
    49261 tgatcgttaa atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta
    49321 ctccattttc ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat
    49381 aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat
    49441 aagttagacc aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt
    49501 tttcaaggac agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt
    49561 ttcctctttc tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg
    49621 ttacttgtct aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt
    49681 ttttcggata caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca
    49741 cgccatccac catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc
    49801 tggaaatatg agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag
    49861 gtgcttcttc cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt
    49921 gtttgtcaat acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa
    49981 tccattcaat cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat
    50041 ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa
    50101 tattttcttc accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag
    50161 atgagaaacc ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt
    50221 cggctttgac tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta
    50281 cactcattcc cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt
    50341 aataaaacag cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca
    50401 ttcgtttgcg ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc
    50461 tctttcggtt tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat
    50521 tcataggcag agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact
    50581 actacatatt gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt
    50641 ctcttacttg cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag
    50701 gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat
    50761 ttgcttacat ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc
    50821 aagtcattag caggagccat atctaaataa tatttccccc cttctatcat ctgatataga
    50881 gcaaaaacag caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac
    50941 ttgaagaagt atggatctct tagataaccg acatttttag gatagatatg aatctcttgc
    51001 agttcctcgg ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct
    51061 gtctgatcga tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa
    51121 taaactgcat cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct
    51181 aatccgccaa ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct
    51241 tctgtgatta gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct
    51301 ttaggagtca tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg
    51361 gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct
    51421 tcctcagaga tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata
    51481 tctcgaatca tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc
    51541 gacatcgcat gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc
    51601 aaacacaaca gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac
    51661 cataggctgc tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc
    51721 cggatcctcc tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc
    51781 tgattcgatt tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg
    51841 cttcttcgtt tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa
    51901 ctacccgatc attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac
    51961 ctggaagttt tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca
    52021 acaagtctga agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga
    52081 tgatttcata ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac
    52141 gtcggctatt catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt
    52201 ctaaggttac ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt
    52261 cgtcttcgat gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga
    52321 acacttgatc agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac
    52381 caatagacag atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg
    52441 cacgagaata gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat
    52501 taccagtgta tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac
    52561 ctgcaccatc taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat
    52621 caaacgtatt gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg
    52681 tgagatattg ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat
    52741 cgtaaagtcg caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct
    52801 ggctctcaat gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa
    52861 atgaatctgg ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc
    52921 cgtcttcatc tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac
    52981 cagaaagccc tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa
    53041 gttcaaactc tgcgccattg attggcatat tactgccttc aattagttta ttgatattca
    53101 tgtggattgg cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg
    53161 tgccttctat gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg
    53221 aattcggatc tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa
    53281 gctcgtagtg acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat
    53341 acgtctcatc tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt
    53401 cgctgactgc tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc
    53461 cgtccgtggt agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt
    53521 catataatgc aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt
    53581 ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag
    53641 tagtgatatc attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc
    53701 aaaaatttcc gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt
    53761 ccccttcacc taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg
    53821 ttgtcggagt cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt
    53881 ctggctcttg gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg
    53941 ccgatctgcc agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt
    54001 gctggatcgt tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct
    54061 tgttgttcgc attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt
    54121 tcaatacatt tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc
    54181 ctaaaccaat ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat
    54241 gataagcaac tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat
    54301 tgttattaga agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct
    54361 ctccataacg atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg
    54421 taactggatg agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga
    54481 acaaaacgtc attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc
    54541 cattagtgct cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat
    54601 ctcccacaac gattaggatt ttttctggat ttctattttc tccgccattg tctttataca
    54661 atgtttcata gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat
    54721 ttaatgctgt attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt
    54781 ccgaactgat ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac
    54841 cgattcgaat atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct
    54901 caatcaacgc agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg
    54961 ctgacttatc tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga
    55021 tgacatctag atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg
    55081 tgctgcttgg cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt
    55141 aactgttttc aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc
    55201 tatcttcttc gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg
    55261 ctcccccgtt tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt
    55321 tgtctccttg atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat
    55381 tcgttgtaaa cttcatctat tctttcatca accaccactt gaagaggtag ttacccttcg
    55441 ttcacatcgg ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa
    55501 taaacttttc cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca
    55561 tcttcatcat aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg
    55621 gctgccgcat cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa
    55681 gccagatgcc attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag
    55741 tcctcatcta agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg
    55801 gtcgtcatat gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac
    55861 tgcaaaagct tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct
    55921 tctttcacaa caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt
    55981 ttaaatcaaa tgatatcctt tttagttaaa aacaccaatt atacaacatt tattattaaa
    56041 attaaattat aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat
    56101 gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca
    56161 ctcatgcttt attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac
    56221 ttcctaaaat agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg
    56281 aataagcgat caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga
    56341 acaacgcagc tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct
    56401 tcggtttact tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg
    56461 tcgttttttt gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg
    56521 ctagaccata ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa
    56581 taaaaccacc attagagata ttactgataa atttcaaaaa attgattttt cgactatccg
    56641 ttttccgttg cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca
    56701 acatgttatt cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact
    56761 tgaaaataaa acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag
    56821 aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta
    56881 aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt
    56941 ttgtaactaa agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta
    57001 tagccaattc ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa
    57061 taaacacttg ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc
    57121 cagaatagtg aaccgagctt tttctcttca ttttttttgt aatgataaat catatacaga
    57181 actgttcgac ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc
    57241 tgtaaatgga taagacgtgg aatccattca atgatttcta agaaataatc attggctttt
    57301 ttaccaatcg atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga
    57361 aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg
    57421 ccatcagata attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta
    57481 acgtgtattt ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg
    57541 cgttgaagga attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat
    57601 gactccaaat atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat
    57661 tgatcgataa gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg
    57721 gaacgatcag gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag
    57781 ggatcatcgt tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct
    57841 ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat
    57901 taccgatcac tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa
    57961 aggcggcaat cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag
    58021 gataacgaac gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa
    58081 tgatcaaaat gattcctatt actgtcatga ggacataact tagtattttt tttgtttgcc
    58141 gattcacact ggtctttttg aaataatcca tcatttgccg tcacctccag aataatagac
    58201 ccacttaggt gcaagcttca aattgacaaa cgtcagaacc aacacgatca gaaacaggat
    58261 cattgacata gccatcgcat aacccatatc aaaagctacg aacgccttgt tccacatatg
    58321 cagattataa aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg tcatgacata
    58381 agcttcttga aaaatttgga aagaaccgat cgtagaggtg atcacatcgt aaaaaataat
    58441 aggagtgatt aatggcaaag taatactttt gaactttccc caaacgcctg ctccatcgat
    58501 ttctgctgat tcatataaag atctaggaac attttgtaat gtagctaagt aaagtagcat
    58561 tgctcctcca gaactccata ttttcatcaa aatcaacgca ggtttcgtcc attgtggatc
    58621 aaacagccaa gctggtccag ggatatggaa ccagcccaga aatgtgttga tcaatcctgc
    58681 agatggagaa agcagctgca tccataagat gtagactgct actcctgata agactgccgg
    58741 taaatagaaa gctgtacgga atacactgat tcctttgatt ttttgattga gtactgtggc
    58801 taaaaacaca ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat aaacggtgtt
    58861 gtataacgat ttccaaaata aatcatcgtg aacgaacatt cttttgaaat ttgctagacc
    58921 aatgaaattc atcttggatg tcaaattata atcggtgaaa ctaccaataa ctgaaaaaag
    58981 cataggccct agcataaata ccaagaaccc gataataaaa ggcgagataa ataaataggc
    59041 ccatcttgct tcttttttct ttcggataga taaacgcttc aaattttctt gcctcctctg
    59101 tacctagaca gtcaaaaatg gattattgat tttgcttgac taagttttct actgcttttt
    59161 gtgcatctgc caatccttct tttggtgatt ttgttcctaa aagaatctgg tcaatctgtt
    59221 cattcactaa tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc accgtatttt
    59281 taaagttttc gtccgccatt tgatagatca tttttccttt atccgttagg ttgtcattat
    59341 tcactaaatt ctcattggct gtcttgctcg ccatgatatc aaaacttttt tcgccgaatt
    59401 tttcttgtac ttctggagaa gaaagatatt tgatgaattc ataagaggcc tcaggatctt
    59461 ttgctccgta tggtacttcc aagacgaatc cgccacccca cgaccaatgg cctgaacctt
    59521 cttctttttc tgggagctgc atcacgccaa aatcaaaatc atccggtgca ttttccatca
    59581 aactagagta ataattgata ttttgggcac gcatggctac taatccggaa ataaatggat
    59641 cagatacacc agacccaaat tcggcttcta atttattgat cgtatttttc ccataataat
    59701 cttgccagtc ttggatccac tccaaggctt ccactttatt tttagtatca atcttgactt
    59761 tttcatcttt gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat acatcagccc
    59821 ctaaattcca taatggataa aaaccgatgc gtgtaaattc ttttccttct tttttatcta
    59881 gtttacgtgc tgtttcttct aattcatccc aagttgtcgg tagatcttgt tcactgattc
    59941 cagcttcttt aaataacttt ttattataaa aaatgacttg tgtatctgta ttgaatggga
    60001 tcgcataagg atcaccttca tattctactg tatcccaaag ctgcggataa aaatccttag
    60061 aagtgtcttt ttctacatat ttggagagat tagttgcttg ctgtgcttct gctctttggg
    60121 caacggaatt tatgtcctgt acaataacat cgggtgcatt tccagctgtt attgcagata
    60181 aagatttagt ccagatgtca ccccatggct gatacttata atttacatgg atctcgtttt
    60241 gtgattgatt gaaatcctca atgatttctt cgatagcttc ttttcgtgca ccggatcccc
    60301 aaaaggacca gaaatcaata ttttttcttc catcacttgt tttttcagaa gaatttgctg
    60361 cacttgaact ggcatcgctt gcattacttg atccgcatcc tcctaataaa aacaatgctg
    60421 aacttgccag caaccctgta aacaataatt ttttcatatt ttacctccaa attttaatgt
    60481 aaccgatgcc ggttttaata taacgatatc attttgttct gaaaataaca atttataagg
    60541 tatgattcta aaaaaattat taatgatacc aatactttat ataattcgcg agcgctttct
    60601 ttgaattttt taatctttcg tagcagaatg agaatgtatt aaaaatagat taggaggaat
    60661 tttgatgttt agacaccaga aagaattgca gtttgaagca aaaccagatc gtccgaatcc
    60721 attaatcgcg aaatatctgc aggagctgat cggcggccaa tatggcgaga tgagtgtagc
    60781 tatgcagtac ctgttccaag gctggagctg ccgaggagaa gaaaaatata aagatatgct
    60841 gatggacatt gcaacagaag aaatcggcca cgtggaaatg ttggcgacaa tggttgctcg
    60901 tttattagag ggtgcgcctg ttgaagatca agaagcagcg atgcaaaaag atccaacaat
    60961 cggtgccatt atctctggaa tgaatccaca gcacgcgatc gtagctggct taggacctcg
    61021 tcctgctgac agcgtgggca atccatggag cggtgcctat atcattgcga gtggcaactt
    61081 gttagcagac tttagagcta atttaaatgc cgaatcacaa ggaagacttc aagtagcacg
    61141 tatttacgaa atgatcgatg atcgtggcgt aaaagatatg ctgtctgttc tccttgcaag
    61201 agatagctat catcaaaatc tatgggaatg tgcagtgaaa gaattagaag aaaaagaagg
    61261 ttcaatctta gtcccaagta ctttcccgcg tgacaaagaa cgaaaagaca ttgcttatga
    61321 cttctataac ttctcagagg gagaccaaag tagtgaaggt tcttgggcta gtggcaaagc
    61381 actggatggc gaaggcgaat atcaatggtt gaaagaacct aagatcgaag gcgctgcacc
    61441 aaaattagaa ccagtaacac caaaaatgta tgggacacct ccattcaaga aataaatatc
    61501 tacccgtaga aacacccgtt ttttgttcca cacgctttgt gagcaaaact gactaaagtc
    61561 attaatcata accacccttc aaaagggtgg ttttctctag agctataagc tcaaatcacc
    61621 ggccagcgcc taaagacgct ggctttcata ttttatgttc aagccatatt gcctttgcca
    61681 gttttgccca cctcttaaaa ggtttatgta ctactcctta gcccttaaaa ggatcgtctt
    61741 actctcgtac actcagctta tctaacgcac tatcatgttt ttcttgttct tgaatgtact
    61801 ttttgatggt cgcttcattt tgtcctactt ttgtcacata gtatccttcc acccaaaagt
    61861 gtcgataccc aaatttatat tttagatttg cacaatctct tgatgatctc ctgaatattg
    61921 gctcgatatt gattataaat tatttttctt ctatactttg gtgtgaatac aatgtggtat
    61981 ttacacatct actttgtacg ggctaaacta tttcccttta ttgctccacc cgcatagcaa
    62041 gtggttttgt atttcaatcg ctcggcgcgc tcaattaggt ctaaagacaa taaatctaaa
    62101 aacccgctgc ttcactgaca gaaacaacgg gttttcagaa aaaaatgaaa tgaaaaagat
    62161 ctcttacggc aatctatttt atttttttga ttttttataa atggaaaatg cttcaagaag
    62221 tccaagaa
//
LOCUS       DACDVT010000006        61067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDVT010000006 DACDVT010000000
VERSION     DACDVT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.006"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..282)
                     /locus_tag="IWL52_000354"
     CDS             complement(37..282)
                     /locus_tag="IWL52_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776945.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(397..975)
                     /locus_tag="IWL52_000355"
     CDS             complement(397..975)
                     /locus_tag="IWL52_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9776946.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1052..1582)
                     /locus_tag="IWL52_000356"
     CDS             complement(1052..1582)
                     /locus_tag="IWL52_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9776947.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1586..2446)
                     /gene="rlmB"
                     /locus_tag="IWL52_000357"
     CDS             complement(1586..2446)
                     /gene="rlmB"
                     /locus_tag="IWL52_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9776948.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2439..2834)
                     /locus_tag="IWL52_000358"
     CDS             complement(2439..2834)
                     /locus_tag="IWL52_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9776949.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2831..4243)
                     /locus_tag="IWL52_000359"
     CDS             complement(2831..4243)
                     /locus_tag="IWL52_000359"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9776950.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4240..4776)
                     /gene="cysE"
                     /locus_tag="IWL52_000360"
     CDS             complement(4240..4776)
                     /gene="cysE"
                     /locus_tag="IWL52_000360"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9776951.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5098..6558)
                     /locus_tag="IWL52_000361"
     CDS             complement(5098..6558)
                     /locus_tag="IWL52_000361"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9776952.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6728..7885)
                     /locus_tag="IWL52_000362"
     CDS             complement(6728..7885)
                     /locus_tag="IWL52_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9776953.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7919..9289)
                     /gene="radA"
                     /locus_tag="IWL52_000363"
     CDS             complement(7919..9289)
                     /gene="radA"
                     /locus_tag="IWL52_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9776954.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9352..9879)
                     /locus_tag="IWL52_000364"
     CDS             complement(9352..9879)
                     /locus_tag="IWL52_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9776955.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10109..10915
                     /gene="proB"
                     /locus_tag="IWL52_000365"
     CDS             10109..10915
                     /gene="proB"
                     /locus_tag="IWL52_000365"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9776956.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10912..12159
                     /locus_tag="IWL52_000366"
     CDS             10912..12159
                     /locus_tag="IWL52_000366"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9776957.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12235..12579
                     /locus_tag="IWL52_000367"
     CDS             12235..12579
                     /locus_tag="IWL52_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776958.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12696..13022)
                     /locus_tag="IWL52_000368"
     CDS             complement(12696..13022)
                     /locus_tag="IWL52_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776959.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13395..14596)
                     /locus_tag="IWL52_000369"
                     /pseudo
     CDS             complement(13395..14596)
                     /locus_tag="IWL52_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14713..15228
                     /locus_tag="IWL52_000370"
     CDS             14713..15228
                     /locus_tag="IWL52_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9776960.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15427..15873)
                     /locus_tag="IWL52_000371"
     CDS             complement(15427..15873)
                     /locus_tag="IWL52_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9776961.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15920..17398)
                     /gene="msr"
                     /locus_tag="IWL52_000372"
     CDS             complement(15920..17398)
                     /gene="msr"
                     /locus_tag="IWL52_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9776962.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17752..18381)
                     /locus_tag="IWL52_000373"
     CDS             complement(17752..18381)
                     /locus_tag="IWL52_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9776963.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(18365..18829)
                     /locus_tag="IWL52_000374"
     CDS             complement(18365..18829)
                     /locus_tag="IWL52_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9776964.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19316..20584)
                     /locus_tag="IWL52_000375"
     CDS             complement(19316..20584)
                     /locus_tag="IWL52_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9776965.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20815..21552)
                     /locus_tag="IWL52_000376"
     CDS             complement(20815..21552)
                     /locus_tag="IWL52_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9776966.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21619..22188)
                     /locus_tag="IWL52_000377"
     CDS             complement(21619..22188)
                     /locus_tag="IWL52_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9776967.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22316..23188
                     /locus_tag="IWL52_000378"
     CDS             22316..23188
                     /locus_tag="IWL52_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9776968.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23530..24402
                     /locus_tag="IWL52_000379"
     CDS             23530..24402
                     /locus_tag="IWL52_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9776969.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24399..26357
                     /locus_tag="IWL52_000380"
     CDS             24399..26357
                     /locus_tag="IWL52_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9776970.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26608..27459)
                     /locus_tag="IWL52_000381"
     CDS             complement(26608..27459)
                     /locus_tag="IWL52_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9776971.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27582..28049
                     /locus_tag="IWL52_000382"
     CDS             27582..28049
                     /locus_tag="IWL52_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9776972.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28312..28794)
                     /locus_tag="IWL52_000383"
     CDS             complement(28312..28794)
                     /locus_tag="IWL52_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9776973.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28894..29757)
                     /locus_tag="IWL52_000384"
     CDS             complement(28894..29757)
                     /locus_tag="IWL52_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9776974.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30355..31203
                     /locus_tag="IWL52_000385"
     CDS             30355..31203
                     /locus_tag="IWL52_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776975.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(31374..31715)
                     /locus_tag="IWL52_000386"
     CDS             complement(31374..31715)
                     /locus_tag="IWL52_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9776976.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            31801..32508
                     /locus_tag="IWL52_000387"
     CDS             31801..32508
                     /locus_tag="IWL52_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9776977.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            32495..32818
                     /locus_tag="IWL52_000388"
     CDS             32495..32818
                     /locus_tag="IWL52_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9776978.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(32907..33944)
                     /locus_tag="IWL52_000389"
     CDS             complement(32907..33944)
                     /locus_tag="IWL52_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9776979.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(34391..35374)
                     /locus_tag="IWL52_000390"
     CDS             complement(34391..35374)
                     /locus_tag="IWL52_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9776980.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(35790..36560)
                     /locus_tag="IWL52_000391"
     CDS             complement(35790..36560)
                     /locus_tag="IWL52_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9776981.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(36553..37488)
                     /locus_tag="IWL52_000392"
     CDS             complement(36553..37488)
                     /locus_tag="IWL52_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9776982.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(38082..39731)
                     /locus_tag="IWL52_000393"
     CDS             complement(38082..39731)
                     /locus_tag="IWL52_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9776983.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TILAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(39926..40822)
                     /locus_tag="IWL52_000394"
     CDS             complement(39926..40822)
                     /locus_tag="IWL52_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9776984.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(40815..41495)
                     /locus_tag="IWL52_000395"
     CDS             complement(40815..41495)
                     /locus_tag="IWL52_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9776985.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(41496..42278)
                     /locus_tag="IWL52_000396"
     CDS             complement(41496..42278)
                     /locus_tag="IWL52_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9776986.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(42271..43188)
                     /locus_tag="IWL52_000397"
     CDS             complement(42271..43188)
                     /locus_tag="IWL52_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9776987.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(43191..43475)
                     /locus_tag="IWL52_000398"
     CDS             complement(43191..43475)
                     /locus_tag="IWL52_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9776988.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            44263..47490
                     /gene="fss3"
                     /locus_tag="IWL52_000399"
     CDS             44263..47490
                     /gene="fss3"
                     /locus_tag="IWL52_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9776989.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            47602..47916
                     /locus_tag="IWL52_000400"
     CDS             47602..47916
                     /locus_tag="IWL52_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9776990.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            47929..48303
                     /locus_tag="IWL52_000401"
     CDS             47929..48303
                     /locus_tag="IWL52_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9776991.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            48313..48657
                     /locus_tag="IWL52_000402"
     CDS             48313..48657
                     /locus_tag="IWL52_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9776992.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            48726..49742
                     /locus_tag="IWL52_000403"
     CDS             48726..49742
                     /locus_tag="IWL52_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9776993.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            50336..52210
                     /gene="ltrA"
                     /locus_tag="IWL52_000404"
     CDS             50336..52210
                     /gene="ltrA"
                     /locus_tag="IWL52_000404"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9776994.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            52336..52692
                     /locus_tag="IWL52_000405"
     CDS             52336..52692
                     /locus_tag="IWL52_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9776995.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            52737..53879
                     /gene="dcm"
                     /locus_tag="IWL52_000406"
     CDS             52737..53879
                     /gene="dcm"
                     /locus_tag="IWL52_000406"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9776996.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            53869..54158
                     /locus_tag="IWL52_000407"
                     /pseudo
     CDS             53869..54158
                     /locus_tag="IWL52_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            54414..55598
                     /locus_tag="IWL52_000408"
     CDS             54414..55598
                     /locus_tag="IWL52_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9776997.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            55613..55708
                     /locus_tag="IWL52_000409"
     CDS             55613..55708
                     /locus_tag="IWL52_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9776998.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            55737..55958
                     /locus_tag="IWL52_000410"
     CDS             55737..55958
                     /locus_tag="IWL52_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9776999.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            55971..56474
                     /locus_tag="IWL52_000411"
     CDS             55971..56474
                     /locus_tag="IWL52_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9777000.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            56601..57638
                     /locus_tag="IWL52_000412"
     CDS             56601..57638
                     /locus_tag="IWL52_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777001.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            57653..59350
                     /locus_tag="IWL52_000413"
     CDS             57653..59350
                     /locus_tag="IWL52_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP9777002.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            59343..59804
                     /locus_tag="IWL52_000414"
     CDS             59343..59804
                     /locus_tag="IWL52_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777003.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            59817..60677
                     /locus_tag="IWL52_000415"
     CDS             59817..60677
                     /locus_tag="IWL52_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777004.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            60715..>61067
                     /locus_tag="IWL52_000416"
     CDS             60715..>61067
                     /locus_tag="IWL52_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9777005.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEP"
BASE COUNT        18470 a        12054 c        11552 g        18991 t
ORIGIN      
        1 caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc
       61 gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt
      121 ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacaccgcg
      181 gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg
      241 accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat
      301 caactatacc acatattttt aaaattttca agtttaccat tataaaaaaa gaaatgcaat
      361 aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa
      421 gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat
      481 tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt
      541 agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg
      601 ctgaatcgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc
      661 tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt
      721 cctcaacaga gaatagattt gattgcttat aatgctttta taataacttc caaagctaac
      781 acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc
      841 ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg
      901 aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa
      961 taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc
     1021 aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca
     1081 atttttggga caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa
     1141 taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat
     1201 ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta
     1261 aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct
     1321 cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct
     1381 tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc
     1441 cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt
     1501 tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta
     1561 caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca
     1621 taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg
     1681 tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa
     1741 tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg
     1801 tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca
     1861 cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga
     1921 ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc
     1981 gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt
     2041 cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg
     2101 taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt
     2161 taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt
     2221 tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt
     2281 ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag
     2341 aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt
     2401 caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact
     2461 ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt caaatgcaga
     2521 tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc agcatttttt
     2581 gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc ttcttctgtc
     2641 aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa
     2701 ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat caggtggtca
     2761 cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc gttcaattgt
     2821 gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct tctaataaga
     2881 tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc
     2941 ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac tcttctgctg
     3001 taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct tgtgccaaga
     3061 cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca agctcataga
     3121 ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg aatcgctgtt
     3181 ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct gaggcagttt
     3241 cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt
     3301 ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc gccatgaaga
     3361 aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg aaattcccta
     3421 gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg tgcatccagt
     3481 aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt tcatgatgag
     3541 gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg
     3601 ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt gaatcccaag
     3661 aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct tctttttgct
     3721 gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg gattggtcac
     3781 ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct gaagattcat
     3841 aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc atatgatcca
     3901 tgactcttgg atgcagtgta gcaggctcga cattcaacgc tttcgtatct tcttcaaatg
     3961 ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt gctgtacgga
     4021 tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag cctcgatatt
     4081 caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg atatggatat
     4141 aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt atagggatga
     4201 attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc tcctcacttt
     4261 ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt cgtctggaac
     4321 atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc
     4381 taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt gtcttttacc
     4441 cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat cgtcctcgat
     4501 ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc cagttccaat
     4561 cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag cattgatctt
     4621 cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc aaaacaaggc
     4681 atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct
     4741 ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct ttcgtaagaa
     4801 ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat aagtttcctt
     4861 atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt atctctgaca
     4921 gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt tttcctgttt
     4981 tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt gataccttgc
     5041 accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc atcgtgttta
     5101 gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt cgatcgtttc
     5161 aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca tgaaaagatt
     5221 ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga cagttggagc
     5281 atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag taggaactgt
     5341 ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat tgaaaaatag
     5401 cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca ggctaacgat
     5461 cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc catcagcgat
     5521 tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga tgtaatggtt
     5581 gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta ggcgttttgg
     5641 atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca caggtgacca
     5701 gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt cgcgatattg
     5761 ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg tttcactgtt
     5821 gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt catagatcat
     5881 cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag tgattttcat
     5941 caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc gtttttgtat
     6001 gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca ccatatctgt
     6061 aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta gaccttgtgc
     6121 ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat
     6181 ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg tgcaatagca
     6241 tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga tttctttacg
     6301 ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat cccaattcat
     6361 tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat tacgtttttg
     6421 gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa acaaataatt
     6481 gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg gtgcatagcg
     6541 tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac taacagcaaa
     6601 aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag cacaaatttt
     6661 accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag ttggctgaat
     6721 ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg tcgattcctc
     6781 gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga agggcacttg
     6841 ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg acaaccatcg
     6901 tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt actactagaa
     6961 cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac gcattgatat
     7021 taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca ttggttacaa
     7081 tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca ctgatatctt
     7141 caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag gcatttagga
     7201 tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt
     7261 gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat ccagtctttg
     7321 taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta tatttatgga
     7381 aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct gtttcttgct
     7441 cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt gtcgttccca
     7501 ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca ctattgacga
     7561 atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc aatccgatga
     7621 tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt ttttgttcta
     7681 cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa aagaaaataa
     7741 gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac tgttgaccga
     7801 ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca atgatcatca
     7861 gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca tttattagtt
     7921 agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc tcgattcctt
     7981 ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg aagccgagtt
     8041 tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct ccagtcagtc
     8101 caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag cttgaagcaa
     8161 tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca cctgcagctt
     8221 ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc actgccatga
     8281 tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt ccgaacatag
     8341 taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc attgccacaa
     8401 cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt gaaggattag
     8461 cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc gttgagccaa
     8521 atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct tcaaaatata
     8581 acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct tctttggtaa
     8641 catgaccgac gataaaaatg gctatcccat ttgttttagc gattttgagt agttctgcag
     8701 tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc tgcgtcatcg
     8761 tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca cgactgattt
     8821 cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg aggcgttccg
     8881 cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact tttccgccta
     8941 tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata cctggatcgc
     9001 caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg ttcagttctt
     9061 ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt tttgttggtt
     9121 ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct tgttcgattt
     9181 cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca
     9241 catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt gtaccgcctc
     9301 cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt tttatttact
     9361 ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg cttttaaaaa
     9421 cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat ccttgaaacc
     9481 aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt aataatcact
     9541 gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg
     9601 gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag taggcacgag
     9661 ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat ctgcgactgt
     9721 ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct cttctgctgc
     9781 ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga tattttgatc
     9841 tttatattta gaaataactt caaatcctct ttttctcacc ggttttcctc cctaaataaa
     9901 ctactcctat tctaacatat ccgaggctgt aaaagacttt ctttacaaat tcttcaagaa
     9961 attctgctat cttaaagtaa accattaatt cgttcttttt ccaaacgact gcaagttgta
    10021 caaaaaaact attttttaca tgactttact cgtcaaaaca agtagaatag aacgaaatta
    10081 atgagtggat atttgattga ggtgtcacat gcgtaaagaa attaaaaatg caaaacgaat
    10141 tattgtcaaa gtaggaacaa gtacattgat ttatcccaat ggaaatatca atcttgcggc
    10201 aatcgatcag ttagcttttg tcctttctga tcttagaaat cggggaaagg aagtgatctt
    10261 agtctcatca ggagctgtag gcgttggtat gcataaatta catttgtcag aacgtccgac
    10321 tttgattcct gaacagcaag ctgcggcggc tgttggtcaa gccgaactga tgaatatcta
    10381 taatcaaaga ttcagtgtat ataatcaaca gacggcacag atcctcgtga caagagacgt
    10441 ggtagattat cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc ttttgattat
    10501 ggacacgatc cctatcgtga acgaaaacga tactgtagca gtggatgaat tagatcatct
    10561 gacaaaattt ggcgataatg atcagctgtc agccatcatg gcaaaacttg tcgcggcaga
    10621 ccttttgatc gtcctttctg atatcgacgg gttttattca gataatccga ctacaaatcc
    10681 cgaggctcag atgtatcgct atatcgataa aatcgatgaa aagattctgg cacaagcagg
    10741 tggtgctggt agcacatttg gaacaggcgg tatgcagagt aaattaaaag ccgcttctag
    10801 gatatttgaa gcaaacagcg caatgatttt agcaaatgga aaaaatccgg caattatttt
    10861 tgatattctt gcaggaaaag aagtaggtac tttatttaag gaggacttga aatgactgac
    10921 ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac aactagcact tatgcctaca
    10981 aaccgcaaaa atgagctact tgttcaaatg gcacaaacca tcaaaaagaa tcaacaagcg
    11041 atcattgaag caaatcataa agacttagaa aaagcagtcg aaaacaatat cagtgaaacg
    11101 atgcaagacc gcttgcagct gacacctgag cgaatcaatg ccatggctac agagatcgaa
    11161 aaaattgctt cgttagacga tcctattgga aaagtagatg agatgtggac gaacaccgac
    11221 ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga ccggcatcat ttacgaatct
    11281 cgtccaaatg tcacgacaga tgcagctagt ctagcattca aatcagggaa tgccgttatc
    11341 cttcgcggtg gcaaagaagc attcttctcg aatcaattat tagtccaatt attgcagcaa
    11401 gtactggtca gtgctggcga atctccttat gccatccagt ttgtcgatga tacttctcat
    11461 gaaactgcag gacagttgat gcgattgaca gaatacttag atgtgctgat tcctcgaggc
    11521 ggagcaaaat taatccaacg agtcaaagaa caggcaacgg tacctatcat tgaaacaggt
    11581 acaggaaata atcacgtata tatcgataaa gaagcacagt tatctatggc agtgaacatc
    11641 atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg cggcagaaac attgctgatc
    11701 cattctgaga tcgcaccttt tttcttgcca gctattgaaa aagaactggt agaacacggg
    11761 gtctctttac gtgcggatgc tcgtgcgtta gaatatctag agacggctgc tttagcagaa
    11821 gacacagatt gggatactga atatctagat tatatccttg ctgtcaaagt ggtcgattct
    11881 ttggatgaag caattgcaca tatcaatcgg cataatacta aacattcaga aacgatcgtg
    11941 acagatagtt atgcggctag tcaacgtttc ttaaatgaag tggacgcggc ggctgtctat
    12001 gtcaatgcct ccactcgttt tacagacgga tcggtatttg gttttggtgc agagattggt
    12061 atctctactc aaaagttgca tgcccgtggt ccaatggggt taaatgaatt aacttccaca
    12121 aaatatatca tttatggcga tggacaaatc cgcgaataat gtttcgtgtg aaacattatt
    12181 cccgcctttt gccttttttc tgtaaaggtt ctatccaact agaaaggtgt caatatgttt
    12241 gaaattttaa tgattttttt tatttacctt atttctctga atatcgctgc ttttctggga
    12301 gtcggtattt tatccctttt ctttcaattc aaaaaacgaa gcatagggag tcaacgggaa
    12361 aaatggtcgc agtattttga caaaatcggg caaaaagggc tggttacacg cttgcacatt
    12421 agttacatgg ttgcattatg cttattagcg acaatcaatt actattcttt tttcgatcat
    12481 tcaatcgctt atacgattac gctactgatt gctgggattt ttcacttatc ttataaatat
    12541 cagctgaata aaaaccactt gaatcggact ttccgctaaa aaatatccgt acgataagta
    12601 gagtgccttg aagatcggca aaaaactgcc ggacttttta gaggctgtga caaagatttt
    12661 gtcacagcct ctttttccat ataaacggcg ttcgcctacc agctgcctgg taaatctgtt
    12721 tcctcttcgt taagaagcga aaatccgtca aatgtttcgc catcataata acgaagcagc
    12781 tcatccatgg acaagttcat cgttttttct ttttcttctt ccgctgattc agccagtagc
    12841 tgttcccgat acacctgata cataccagct attttttgct gcatcgcttt tctagtttca
    12901 ccttccgcat tgattattgg caattctttg atatagccac gataaatcaa ccgatcatct
    12961 gaaaattcaa tgggtaattc aacgagtgtc aacccgactt cctgaaaata ttcttccagc
    13021 atctattatc cccccattct ttttcttaaa tgaattttag cttacctact gaaaattttc
    13081 cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac atcccctttt
    13141 tccgaataaa tggtgttctt ctgccaactc ctcaatcata agtcacaata ttcaacaaac
    13201 atgagaaact gttttttgaa attgttcctt gtgattcaga gttaaacgac agtctcacaa
    13261 cctcttggta aacggcgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    13321 aatatgagaa gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    13381 caacctctga tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata
    13441 caacatacac tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata
    13501 tgttcaccaa tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa
    13561 ctatagctgg acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc
    13621 aacagtgtat tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg
    13681 actggcatag aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct
    13741 gctaaaattg tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa
    13801 gcaatagcca gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag
    13861 ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat
    13921 gcgattagtc caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg
    13981 cctacttttc tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg
    14041 caaaacatca aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc
    14101 ccaccgacta gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt
    14161 gctttttctg tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat
    14221 aaagcattcc cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat
    14281 aatccgccaa gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgttttttca
    14341 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    14401 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    14461 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    14521 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    14581 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
    14641 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    14701 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    14761 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    14821 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    14881 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    14941 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    15001 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    15061 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    15121 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    15181 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    15241 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    15301 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    15361 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    15421 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    15481 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    15541 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    15601 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    15661 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    15721 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    15781 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    15841 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    15901 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    15961 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    16021 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    16081 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    16141 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    16201 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    16261 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    16321 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    16381 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    16441 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    16501 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    16561 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    16621 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
    16681 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    16741 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    16801 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    16861 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    16921 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    16981 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    17041 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    17101 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    17161 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    17221 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    17281 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    17341 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    17401 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    17461 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    17521 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    17581 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    17641 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    17701 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    17761 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    17821 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    17881 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    17941 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    18001 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    18061 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
    18121 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    18181 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
    18241 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    18301 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    18361 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    18421 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    18481 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
    18541 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    18601 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    18661 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    18721 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    18781 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    18841 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    18901 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    18961 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    19021 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
    19081 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    19141 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    19201 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    19261 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    19321 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    19381 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    19441 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    19501 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    19561 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    19621 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    19681 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    19741 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    19801 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    19861 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    19921 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    19981 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    20041 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    20101 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    20161 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    20221 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    20281 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    20341 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    20401 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    20461 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    20521 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    20581 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    20641 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    20701 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    20761 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    20821 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    20881 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    20941 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    21001 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    21061 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    21121 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    21181 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    21241 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    21301 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    21361 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    21421 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    21481 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    21541 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    21601 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    21661 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    21721 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    21781 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    21841 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    21901 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    21961 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    22021 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    22081 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    22141 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    22201 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    22261 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    22321 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    22381 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    22441 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    22501 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    22561 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    22621 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    22681 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    22741 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    22801 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    22861 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    22921 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    22981 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    23041 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    23101 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    23161 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    23221 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    23281 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    23341 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    23401 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    23461 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    23521 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    23581 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    23641 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    23701 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    23761 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    23821 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    23881 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    23941 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    24001 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    24061 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    24121 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    24181 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    24241 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    24301 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    24361 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    24421 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    24481 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    24541 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    24601 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    24661 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    24721 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    24781 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    24841 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    24901 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    24961 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    25021 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    25081 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    25141 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    25201 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    25261 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    25321 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    25381 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    25441 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    25501 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    25561 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    25621 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    25681 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    25741 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    25801 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    25861 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    25921 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    25981 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    26041 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    26101 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    26161 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    26221 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    26281 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    26341 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    26401 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    26461 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    26521 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    26581 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    26641 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    26701 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    26761 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    26821 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    26881 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    26941 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    27001 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    27061 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    27121 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    27181 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    27241 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    27301 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    27361 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    27421 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    27481 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    27541 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    27601 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    27661 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    27721 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    27781 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    27841 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    27901 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    27961 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    28021 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    28081 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    28141 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    28201 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    28261 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    28321 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    28381 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    28441 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    28501 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    28561 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    28621 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    28681 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    28741 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    28801 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    28861 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    28921 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    28981 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    29041 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    29101 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    29161 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    29221 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    29281 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    29341 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    29401 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctactgat
    29461 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    29521 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    29581 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    29641 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    29701 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    29761 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    29821 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    29881 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    29941 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    30001 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    30061 acgctgcttg gatttttctt tgcgatacat catacctctt ttatcaattg gaacaattat
    30121 ttatttctaa caaaatcact tcatttttgt cagataaata aaaaacaaaa aaagttccat
    30181 ctttaaaaca cgctattctt ttactttttc tcacattctc taagaaaaga aaaggagaat
    30241 gaatttttga caattcgagc aaaagtgatt atcaaatatg taaaatcagc tacttttttc
    30301 ttaaacgttt ctttatacta aatataaata agtgtttcat gaaaggagaa ctagatgaat
    30361 aattggcttg agtattttcc tgaaaacgtt ttggaacgcg gttacagcta tcatctgcac
    30421 ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt ttctggcacg
    30481 gaagattata aggtagtgat cacttgggat gaaaagacca acatgacttg tgattgtcta
    30541 tatgccatag aaggaaaaaa atgcaaacac atggctgctg tcttattcgc ttacgaagaa
    30601 cgtccaataa aaaaatccaa ctactcactt tccgaactct cgtcccttgt ttcctccgct
    30661 agttcctctc ttgtacggga gcttttaaca gagattctta tagaacatcc gcactttatc
    30721 gaacgcttca aagttaagat gccatttcat gcaataaatt attcagataa attaacaact
    30781 atcatccata aatacgatca tattatcaag aaaaacaaaa acaggaaaac agcgaagttc
    30841 atcatggaga tgcgaaagtt catccaagaa gcagttgaat cgcttattca gcaaaatgcc
    30901 tatcttcccg cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac attctattgg
    30961 gagcctgagg atgaacgaac ccttcttctg atagaggact gttattattt atggaaagaa
    31021 ttattagcag aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt cgtctgccaa
    31081 gtcgatcata ctgacgcctc ttattcaaaa cgctactcta taaaaatcct gaaagaagac
    31141 tttcgggaaa aagaattttc taaccaaaag aaaaaaatag ataaaaaact aaagaaacgg
    31201 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
    31261 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
    31321 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
    31381 tcatgacaaa tttccttcgt tgctggatag attttccggc attgttgata gattttatga
    31441 tacttttcta cattttgtaa gagaggttcc gctgcatctg tatattggat aaaattctga
    31501 caacaacttt caggtgtgtc gaaccaccct aaccctagtg cagcaatcat tgttgcgcct
    31561 aatgcaggtc cttcctccac acttaaacaa tagaccgttg catcgaaaat atcagcttgg
    31621 atttgtagcc atgcaagatt tttcgcgcct cctcccaccg aaacaatacg tgtaaatctg
    31681 cgacccgcta gttctttacg tttcaatccc accattgaca aatgctcatt aaaaatttag
    31741 aatattcaca agtttaacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
    31801 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgttttcggt
    31861 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccggatt tcatccacta
    31921 gtcgtcacgt tgatgtccct gctggtctat gctggttctg cccaatttat cacagtcagc
    31981 atgcttgcta gtcacagccc attgctttcc atcgttttct cgacctttct agtcaattcc
    32041 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
    32101 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
    32161 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
    32221 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
    32281 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
    32341 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
    32401 attctggtat atgtcggact gatttttatc ccaagcaact tagtgattgt agttgtaaca
    32461 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
    32521 gactatttta ggttgtactt tagcaacttg gctttctagg gtcctaccat ttgtcttact
    32581 gaaaaaattc gatttaccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
    32641 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
    32701 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
    32761 cttattggtg atcgtcttgg caggaattct ttccctttcg ttgattcgct tattataaat
    32821 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
    32881 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
    32941 cctaacatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
    33001 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
    33061 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
    33121 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
    33181 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca
    33241 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
    33301 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
    33361 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
    33421 agttgattca aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg
    33481 cgattggcat tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc
    33541 aatgaggtca acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg
    33601 acaatacgtt cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct
    33661 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact
    33721 ttcccgactc catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga
    33781 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
    33841 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac
    33901 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
    33961 cttcatcaag ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt
    34021 tccacctttt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
    34081 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc
    34141 taccaactct tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa
    34201 ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc
    34261 aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt
    34321 tggctgttct tattacttga tacaggacag ctttttcacg acctctaatt cacggtgttc
    34381 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtctctaaga
    34441 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
    34501 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
    34561 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
    34621 tttctgtatg ttccctcggg tctttttcaa atgcataagg attcttgaaa atccctcggc
    34681 caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg
    34741 ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga
    34801 ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
    34861 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
    34921 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg
    34981 cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt
    35041 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
    35101 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
    35161 tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat
    35221 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
    35281 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
    35341 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
    35401 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
    35461 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaaagactg tgacaaaaat
    35521 cttatcacag tccattttcc ggaataaatg gtgttcaaaa gcttgcattt gcggtaataa
    35581 gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    35641 cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    35701 tcgctcaaaa aacaacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag
    35761 gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc
    35821 aaataccaag taacggcgta caaaacaacg atgaataaca caagtacgat catcgatgtt
    35881 gctactggca catcgatcgt tccaaggaat ccgtaacgga aaccggagat catataaacg
    35941 atcggattga cttttgaaac agcttgccaa aagggcggta acatagagat ggcatagaaa
    36001 acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcatca
    36061 taagactgag caaaaatccc gttgagcagt cctgctaatg aaaacaagat tgccgtcatc
    36121 aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa
    36181 atgatcgtta cgagagagcc gacgagaata ctgcgaccca caccaccaat cacaaagccc
    36241 caaataatca catgcgtagg aacaggtgct accaagattt cttcgatatt tttctgaaat
    36301 ttttgagaga agaaggacga agaaacattg gcatacgaac tagtgatagc agacatcata
    36361 atcaaaccag gaacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgccca
    36421 ccgatcattt ttccgaaaat aacaaaatac aaggatgtcg tgatcactgg cggaaccagt
    36481 gtttggaccc atatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca
    36541 gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa
    36601 gaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct
    36661 tgtaagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt
    36721 ctgctcatct tcaaatgtac aagcatatcc ttcaatcaca ggtttggtat catatggcgc
    36781 taaatcaaag ataaatgttt caaattgcag ttttgacaga agctctttca tacttgtatt
    36841 ctcgatcagt tcacccgatt ggatgattcc gatgttgcga caaagcattt cagcttcttc
    36901 tagataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc gtagaaactc
    36961 ccacatctcg cgtctcaact cgatgtcaac tcctgctgtt ggttcatcca agatcagcaa
    37021 acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag
    37081 catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct
    37141 gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg
    37201 ctggaccgtt tcaaaagggt taaaattgaa ctcttgtgga accaatccaa tctgttgctt
    37261 tgctcgaact agatccttgt ccagatcata accgaagaca ttcacttttc cagatgtttt
    37321 attgactaaa gaagtaataa tcccgatcgt tgtagacttc ccagctccat ttggccctaa
    37381 tagcgcatag aaatctcctt ctttcaccga aagatcgatt ccccgcaatg cttcgacccc
    37441 acttccatag atctttttca aattatttat ttcaagcgca tttgccattt atttcctatc
    37501 tccttataga catgattctt aattgtatca tggaatacag cagatgaaaa aacaacttgg
    37561 cttccacaca aaaacacacc tgctaacaaa gaggagatgt gttttctagt tcatgatcat
    37621 cttcgataga ataactatct aaacaactct ggaactttta aaatcataaa agtaccgaca
    37681 gcaactacta cgatgacata tagaagatcc ttataactga ggttcttgtc ttttttattc
    37741 atgttaagct ttcctccgtc cttttatcga aagctttttt cttttatttt aaccgaaata
    37801 aataaaatgg aaatagttat tttcaatttc tgttttcgct gtttgtttta cggagaactt
    37861 tgccatggaa cgtgcgtctc gtcgctgatg ctaagttttt tcgataaaag ctgctagaag
    37921 gaagacagcg tggtttattt tgtatcgtct tttgctaaat cttccaagat catctggaca
    37981 gcagactcgt tcccattgaa ttctgccact tttttggcat caatgtactt cttttgataa
    38041 tcgtcttttt ggacccacgc atcatgcttc gtttgataaa gttaatcaaa cggataccac
    38101 tgacgctgtt catcgaacaa caaactttct ttttgggcct gttcaactat ggatttagga
    38161 taattcaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat
    38221 ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca
    38281 caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat
    38341 ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt
    38401 gttcctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc
    38461 agcaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca
    38521 tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc
    38581 gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat
    38641 tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac tggattaact
    38701 accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac
    38761 tctggtcggc aagtgatcgg catataggtg cgataattca aaacggctgc tgcaacttcc
    38821 aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg
    38881 agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc
    38941 aattctgctt cactcccgct cttcattcga aatgaaaaac ctaatttagg gatgtctctt
    39001 aatggtttag tttgctgttt cagctctttt tgaagtggtg tcgtcatttt tgctaactga
    39061 ttggcggcta aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt
    39121 tgtttcttca ctagataata gaccgtattg aatactaagc caccgattgc tagatagatt
    39181 cctagtactt gccctaaaag aagcaaacct acacccacca gcggaagcaa gccaagaaaa
    39241 ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca
    39301 aaattatgat ctttttttcc taaacgagcg aattgatatt ggattctttc tctcagttga
    39361 ggattttcct gataaaaagc aattagtttt tctaactgat aatcttctcc aaaatcaaag
    39421 ctacgtaaac gctgatacaa cgcttctgaa cctacactag aataggtact gttgatccct
    39481 tcaaatactt cgaacatatc caagtcatac caagtaagat cgtcgatttc actatcccat
    39541 gaacggaatt ttttctccgt gagccacgca tccttcaaac tttcttcttt atcaaatctc
    39601 ggttgataag gaatttttcc ccaattacta cgaactgttt cctttacttt caaacgtgaa
    39661 aaaatgtcta aaacaactaa taaaataaca actgcaataa taattaaaac gataaattct
    39721 ctttggccca tatccctctc cgttccttga tttgttagtt ttagtatagc actgttgcgc
    39781 tatttgaaaa gggggtacaa aaataactgt cttcgttacc taattcgtat caatcacacc
    39841 aaaataaaaa caccttccaa aaaagaagat gtctttaaaa cgagatacaa ttttttgtca
    39901 ttcacttcag gtaaattaga gccaattaaa ccctccgcaa cttgacggag atggagaaga
    39961 tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca
    40021 ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac
    40081 gctggaagac tttttcaaca gaggtttgcg ctcctggttt gataggattg gccactgtta
    40141 atcgaatgaa tgaatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca
    40201 ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag
    40261 ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat
    40321 aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt
    40381 ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc
    40441 ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct
    40501 gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa
    40561 ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt
    40621 cattttcatc cacccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa
    40681 attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag
    40741 ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga
    40801 gaagcaacag ccacttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca
    40861 gcatgtgcct ttgctagttt ccctcgaaga ttggaaatat gcatattcac tgttttctca
    40921 ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt
    40981 ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc
    41041 acaccattca catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg
    41101 cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt
    41161 aacaacagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac
    41221 aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat
    41281 gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga
    41341 aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca
    41401 tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aagaatgaac agcttcatcg
    41461 tcttcgataa tcaaaatagt ttccagcagg aacacctact taatgtcaga tttttggaaa
    41521 ataaacattc ctagagctga tgataccacg attgttacta gactgaccag aatcattttc
    41581 ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta
    41641 aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg aacgaaggcc
    41701 gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat
    41761 gtcaaagcca ctggaacaga taatgtcgct aatatcagca gaagcaatac aggaaatact
    41821 tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt
    41881 gtgtaagcaa tcatggctag ccccgtaatc aaaaccacgc caagtgttgt gatcaaaagc
    41941 gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg
    42001 cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc
    42061 agaagaatcc atgcagccat tttttccacc attaaaatcc ctgttgcacc gttgatgact
    42121 gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa
    42181 gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc
    42241 catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg
    42301 taagaaataa tcttccagtt tttgcccaac taggtggata ccatccacag gaacttttgc
    42361 ctctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac
    42421 acggctagat tggacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg
    42481 ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt
    42541 cgataattct ttgatcaagt gtccttcatg caaaataccg tagtgagtgg cgatctgtga
    42601 caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg
    42661 ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc
    42721 caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat
    42781 acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag
    42841 caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc
    42901 tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct
    42961 tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa
    43021 acccataata gttctcataa aggtagactt acccgctcca ttctccccaa tcaaaccata
    43081 gatcattcca cgtttgattt cgatcgatac tttatctaat gctttggcag caccgtattt
    43141 tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct
    43201 ttcttattga taagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa
    43261 agaaagtgta aattttccaa ttgaatttca agaaaaatca gactgtctcg cagacgtggt
    43321 tgagttgtct gccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
    43381 atggtaatcg ttgttatcgt tgacatcaga cgtccttgta tatgcgttgg aacttctcgc
    43441 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
    43501 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
    43561 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    43621 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
    43681 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
    43741 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
    43801 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
    43861 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
    43921 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
    43981 ggttttttct tcgtatcatt gaataagttt gaaaagcctt gatttataaa ggtttttccg
    44041 taaatatatt ttgttatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
    44101 atttaaaggc agtatatttc gtattttata ttaaattttt aggctcattc attctcaaat
    44161 ccattctatt tagtcaataa atattttaaa aagactttat aaacaaagat gttcaagcga
    44221 gcatcttatt tttttgtgcc taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
    44281 aagaaattat gtgtattaac tattttgagt ttggctgttc aatcaattgt aggagcaatt
    44341 gctcctacga ttgcttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
    44401 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
    44461 gtcactgttc cgcattatgc agtgtacaac ggcgttaaac aagatatttt ctgtgttgaa
    44521 ccaggagtcc cgatttacaa tgagttcact cctggatatg agaagaaccc gttaccagat
    44581 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
    44641 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
    44701 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aatcaatcaa
    44761 gccattgccg attatcagaa gaaaccaagc ttccataata gtacggctaa aacagtatta
    44821 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
    44881 gtggaaaata ccgcaaacat tgattatcgt gtgaatggca atcagttgat aatcagccca
    44941 aatgccaatt caaaagaaag tggtgtgttg acgcttaaga aatctgctgg taccggaaca
    45001 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat cgataagccg
    45061 aacacttata cggtcaaaat tgatgtggaa atggagggtt ctttgaagat taaaaaagtt
    45121 gataaagaat cgggtgatat tataccagga acggttttcc atttagactt tggaaaaact
    45181 ttacccgcaa aagacgtgac caccgataaa gaaggcattg cgaccttaga tgagattcct
    45241 catggtacaa aggtaaccat tactgaaaaa tcggtgccgg cgccttatac gattgatacc
    45301 acacccatga ctgccacaat taaagcaggt gaaaccattt cagtgacttc aaaaaatacg
    45361 cgagaaaaag gtcaaattat tctggataaa accggaattg aaacagggag tgatctttgg
    45421 aacgataatt actctttagc gggcaatacg tttgccattc gcaaggatag cccaactggt
    45481 gaaatcgtcc aagaaatgac cacggatgaa aaaggtcatg cagaaacacc aaaagagatt
    45541 gccaatgcgt tggaattggg aacctactat gtgactgaaa ccaaggctag tcatggcttt
    45601 gtgaatactt tcaaaccagt aaaagtcgaa ttgaattatg tcaatcaaac agtggctctt
    45661 gttaccagta acgtaaaagg gcaaaaccaa gaaattactg gtgaaaccac tttgacaaaa
    45721 gaagacaaag ataccggtca tgaaactcaa gggaaagctg agtttaaaag agctgaatac
    45781 accttgttta ctgttaagga tggaaaagcg gtcaaatgga ccgagaactt taaaccagaa
    45841 ttgttgaagg gaacgaaagc ttctgatgaa accgtaactt tgactttaga tgaaaagaat
    45901 caagttgccg ttaaacactt agcgattaat gagtattact ggcaagaaac caaagcgcct
    45961 gaaggatata ccttggatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
    46021 gaaaaaaatg ctgtgattac tcgagatgtt gcggcaaaag aacaagtgat tcgctttggc
    46081 tttgatttct ttaaatttgc tggatcaact actggcactg ccgaaactgg cttcaatgat
    46141 ctaaccttta aagtgtcgcc attggaagga accaatgaaa tcaccggtgc cgaagatgaa
    46201 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
    46261 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
    46321 ccattagaaa tccgttctac ttttaaagaa aataaagagg attataccaa gagtgattat
    46381 atctttacta ttaccgaaaa agggcaaaag caaccgatta aaacggtaac ggtcccttat
    46441 gggaatctaa ccaatgagaa tttctcagtc agtttgaacc gtttgatgct ttatgatttg
    46501 cctgaggaag aagatagctt aacttctctt gcgacttgga aagaaggaga taaagaattg
    46561 actactcttg attctaccga gctagtcgat aaattgagct ataatctaca tgaaatcaaa
    46621 gaagactggt atatcgtagc tcaagccatc gatgtagatg ctacaaaagc tgcccaagaa
    46681 aaagataaaa aagccaaacc ggtggcgatt gcggaaacaa ccgcaacttt agcgaataaa
    46741 gagaaaacag gaacttggga gattctgcat aaattaactg tcgaacaagt tttagacaag
    46801 accattgtct tattcaacta tgtgtatgaa agcaaagaag cctatgaagc tggtgatgag
    46861 ccggtagcga aggatgctag cttgaacaat caagcgcaaa ctgttaattg tatggtggaa
    46921 cgtcatgttt ccatccaaac caaagcgcac ttagaagaag gttctcagac ctttactcat
    46981 ggtgacgtgg tagatatgtt tgatgatgta tccattaccc atgatgtact agatggctca
    47041 aaagaagctt ttgaaacaat tctgtacgct ttatttccag atggtacaag taaagaaatt
    47101 tggaaatctg gcaagattga ttacgaagtg aatgacaaag aatttaccaa aaccgtactt
    47161 gcggaaaaag tggatacaag taaatatcca gaaggtacaa gttttacctt caaagaaatt
    47221 aactatgaca aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
    47281 caaactttaa cgcccaaaaa agttccgaca acaccaagta caccagaaca atctgaaatg
    47341 ccaactgttc caagtggctc tcaagaatct agtcctacag tgaaggcatt ccctcaaacc
    47401 ggggagaaaa cttccaatgt gctactgttc attggcttta gcttgatctt tgcgacggct
    47461 ggctattatt tttggaatcg tcggaactaa ggtgataaga tgaaacatgt aaatagtaga
    47521 aaacaatacg cgcgaccgcc tcctaaaaaa gtggtcgcgc tatttttgta ttcaaaacta
    47581 acaatgaaaa gaggaaaaaa tatggaattg aaatttgttg taccagatat ggtcgagacg
    47641 ttcggaaaac taacatacgg tggtgaaggt gaagttctat cggaagggta tggtcgaaat
    47701 actacaattg ttggtcgaag ttaccatctg ttttcaaata aacaacgggc cgatgatatt
    47761 gaagtggtgg tagctgcgga agccggcgaa aaggattttg accaagatca accactaaaa
    47821 gctgtaaatc cccacttaat tgccaaaggc tatgaaattg agaatcgtgg gtttaccgat
    47881 tacgtgttgt atgttgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca
    47941 gaaggtattg tcgtagattc aggattaacc tttggcaagt tgcgtttttc cgcattacgt
    48001 cgagaagtac gcaagcaaaa tgaggacgga acggttagta acgaggtaaa agaacggacc
    48061 tataatttga aatcctccgc gcaaggtcga atgatccaag tcagtattcc agcaaatgta
    48121 cccttacgcg agtttgctta tgatgcagag gtagagctgg tcaatccaat cgtggatacg
    48181 gtcgccaatt atgtttttcg ggaaggaacc accgttaatt ggtttatcaa ggcagatgat
    48241 ttagtcttga aacgacaact gaatcaagga aaacctacga accaaaatga aggaaagaaa
    48301 taggtgaaga atatggaaat taatgttgaa catattttag attgtctcga tcaatttggc
    48361 aaaggtgagc taacggagga acaactcact aacgcattaa cctatgatga aaaaatgttt
    48421 ctgattatgc atcaagggtt actggaagta ggtaacgatg ccaaagaaga ctttgatgta
    48481 ctggattggt taggggaaga agagtctttc tttatggttg tcgaggtaaa tgaaagttta
    48541 tgtcggcagg cagaatctgt tttggaagaa atcgatgtgg agatgccgga tgccatcgaa
    48601 agatttttaa aacaattagt ggaaacgaaa caacttcccg tagtagtaaa tgactagcat
    48661 aactttttga aattgcccaa tgaaatccta ttgggctttt tcgactggag gtggaaacca
    48721 tcaaaatgta caaaggacat cgcatacgcg caggagatca acacttgatt tatcactttg
    48781 ttcttggctg gctaagtgcc ttattcatcg gttggatggg cgtcttttat tttccagagc
    48841 ttagacaatt tggtctttct aaggtatcat tttccactat tgaaatcgct tggtccatga
    48901 aagatttagt atgtctgctt ggaagtctcg ctctttcagg aagtatgatg ttgctttata
    48961 ttcatttttt tcctgatcat tggcgaagtt tgtggaatcg acaaaagcta gccagaatga
    49021 ttctagaaaa ccattggtac gaagtgaagc aaactcaaag tgatggcttt ttcaaagatt
    49081 tgaataggag tcgaaccaaa gagactatca gttacttccc caaagtttat tatcgaatga
    49141 aagatggctt actgtctatt cgcgttcaaa tttcactggg aaaatatcaa gatcagctat
    49201 tgaagttgga aaagaagtta gaaagcggac tgtattgtga gttagtagaa aaggaactca
    49261 aagactccta tgtggaatac accttgttat acgatatgat tgccaatcga attgggatag
    49321 aagaagtagt ggcagaaaat gggacgttgc gattgatgaa aaatcaagta tgggcgtatg
    49381 attccttgcc tcatatgtta atcgctggtg gcacaggtgg tgggaagacc tatttcttac
    49441 tcaccattat tcaagcacta ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa
    49501 atgcggacct cgccgattta ggtacggtaa tgcctcatgt ctattctcaa aaagaagaaa
    49561 tttctaaatg tgtggaagat ttttacgaac gcatgatggc tcgtagtagg tcaatgaaag
    49621 aaatgcccaa ctacaaaaca ggagagaatt atgcgtatct cggacttccg cccaactttt
    49681 taatctttga tgaatacgtg gcttatatgg gtgcgaatcg ttttcctaca agcatcgaat
    49741 agatgtgaaa tggaagccta ttgagattat caatagtgac aactaatgtt ttgagagatg
    49801 gaatgaaagc cttcaagttg agctgaaaca tccccgctaa tactcctaca tgctctaatc
    49861 aatgaagtag attggttttg gtatgaagct aggtgaagtc gtctgaaatc tgcctaagtg
    49921 cttgagcata aggctgacta tagggcgggc ggctgaaaaa gataactatg gtaagaatgt
    49981 ctgaaaatga ctgacgaatc tgcgaatgta cgggtctaaa aggctgatct tattgaaaga
    50041 taggaacata cggacgtatg gtgagatacc gacaagtaag gttccatgat atgaaaatcg
    50101 ggatagctaa cgaagaagct ataatttcac aggctcatag tagataccta aggttataca
    50161 tgatagctaa aaacattgga acgatgtaag cagaaaacta tcaattaaat gactacttct
    50221 agataggtgc atataagtaa taacgaaatt gctttgtttc tgtgaaagta agggcacagt
    50281 accgatgaag cgtctaacaa atgtggaggg atagccctaa gtctatttaa taacaatgaa
    50341 tgttgaaaca gatgaagttc aaggatctgg taagaacgtg ggaaaatccg ttatggaggt
    50401 gccacgcttg aacacaaaaa tacgttactg ggaatattat gatctacaag aaacattcga
    50461 tcagctattt actcaaagta gaaacgggaa aaagttttat caattatatg agttaatcat
    50521 ttctgaaaat aatattcttt tagcataccg tactatcaag gcaaacaagg ggtcttcgac
    50581 accaggaacg gattccttca caattgataa ctataaggaa atgaaccaag cagagtttat
    50641 ccatcttatt ctcagtcatt tagaaaatta caaaccaaaa tccataaagc gagtaatgat
    50701 acctaaaccc aatggtgaga aacgcccatt aggaattcca tgcatgattg atcgaatgat
    50761 ccaacagatg ttcaagcaga tactggagcc aatatgtgaa gctaagtttt atgaacatag
    50821 ctacggtttc agaccattga gaagtgcaaa gcatgctcta ggtcgcatta tgtatctcat
    50881 aaacatcagt aaaatgcact atgcagtaga cattgatatc aaaggtttct ttgataatgt
    50941 aaaccaccgt ttactgataa aacaattatg gaatatagga atttgtgaca aacaagtctt
    51001 agctattctt tcaaaatctc tgaaatcacc cattcaagga gaaggtatcc cgagcaaagg
    51061 aacgatccaa ggaggtatca tttctccact attatctaat gttgttctaa acgacttaga
    51121 ccattgggtc tcaaagcagt ggcatacctt tgaaacaaag tatccctata ctaaaggtta
    51181 caataaattt cgtgctctaa gagatacaaa tctaaagcaa ggctatattg tcaggtacgc
    51241 agatgacttt aaaatcatga ctaatgatta tccgacagca ttaaaatggt tccacgctgt
    51301 caagctttac ctaaaagaca gacttaaatt agatatttcc aatgagaagt ctaaaatcgt
    51361 caatttgcgt aaatgtaagt cggaattttt aggattcgct atttgcgtga aacaaaaagg
    51421 gaaaaaatgg gtttgtaatt cacgtatttc taacaaaaaa aaggatcaga ttaaagaaga
    51481 gattaaacaa agaatcaaag atattcaaaa gagtcctaca gcacaaaatg ctctcctgtt
    51541 taatgctctc gtgctcggtg ttcataatta tttcaggtat gccactcata ttaatttaga
    51601 tttgaatagg atgaactacg ttctttcatt cactacttac aaccgattat caaattgtag
    51661 tacaagatgt taccccaaca aagcttcccc aacttacaaa aagttttact caacaacgat
    51721 gaaaacgtat aaagtagctg gagtctattt gtaccctctt tgcgacgtat ctacaaaaac
    51781 aatttacgga tttaatacgg aggatacacc gtttactccg tttggtcgtc aaagattgga
    51841 gcacaaaagc ctacagtctc ttgtgttcca agaattaaga aaattgatgg aatcgaaaat
    51901 accgaataga acggttgagt atttagatag tagaatatct cgttactcaa tgaaatcagg
    51961 caagtgtgag attacaaaac aatttttgcc agcaaaagcc gttcactgtc atcactattt
    52021 gccaaaaagt cttggtggag acgacaaatt cgataacttg agaattattc ataaggatat
    52081 ccatcttctg atccatacaa caaataaaat gataatcgat cactacgtgc atgaactgaa
    52141 actggtgcca gagcaaatag caaaaatcaa cctttatcga aagatgtgta atctgcaaaa
    52201 cattcaatag aaaataaata gatggaacgc cgtatgaggt gaaagtctca cgtacggtgt
    52261 ggagcggggg aaaagatgga gattatatca aattcttacc tatcgctata gatgttaacg
    52321 acaaaagaaa gtgcggtgat tttgaataag cttaaacaaa tcgtgatgtt aggccgtcaa
    52381 tctggtttct ttctgattct agcttgtcaa aggccggatg cgaaatatct aggagatggg
    52441 attcgcgatc agtttaactt ccgtgtggct ttgggtcgta tgagtgagct tgggtattcc
    52501 atgatgtttg gagaagtcga taaaaatttt tttatgaaac gcatcaaagg acgaggttat
    52561 gtagatacag ggggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat
    52621 gatttcttaa gggaaattag caacattgtt ggattaaccg ttcatacgga aagagaaaat
    52681 aatagtgtgt agcctaggaa tatcaataat cctgggcttt tttattgagg tgatttatga
    52741 catttttaga tttattcgcc gggattggtg gctttcgctt aggtatggaa caagccggtc
    52801 accagtgtat cggtttttgt gaaattgatg aatttgctcg gaggagttat aaagcaatcc
    52861 atgatacaag gaaggaggtg gaaatgcatg acatcacaag tgtatcagac gaatttgttc
    52921 gatccctcgg accagtggac atcctttgcg gcggctttcc gtgccaagct ttttcaattg
    52981 cgggaaagcg gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    53041 tcgccactct tctccaacct aagtttctat tccttgagaa cgtccgggga ttgctcaatc
    53101 acgaaggagg ggctacgttc gagacgatcc tccgaacgtt ggatggattg gggtatgatg
    53161 tggaatggca agtgcttaac tcaaaggcct acgttcccca aagccgtgag cggatcttcc
    53221 ttatcggaca ttctcgagat gcatgtaccg aacaagtatt tcctatcatt ggatcgtctt
    53281 caacatctga tcaaaacatc agaaacttgg taaatatcaa tccttccaat cgaggaatgg
    53341 gtggccaagt ctatggttca gatagcgtag caccaacctt gaccggtgat gaaggaatta
    53401 aaattgcctt accggtaaag gatgggattt ctgtagcggg tatgttgtcg ggaaattttg
    53461 aacaaggcaa taggatttat gaggtaaccg gaacagcccc cactttgtca acgaaacaag
    53521 ggggaacgaa gattatgatt cggaaaaatg aaacagaata tcaagaggtg aagccagggg
    53581 acagtgtgaa cctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaaa
    53641 gtgtgcatac cttactaaca ggagaccaac aagcggtagt cacggatcag taccagattc
    53701 gaaagttaac acctagggag tgctggcggc ttcagggctt tccagattgg gcttttgatc
    53761 gagcttctca agtcaattca gatagtcagt tgtataaaca ggccggaaac tcagtaacag
    53821 ttccggtcat ttttgatatt gctagaagat taaagggggt aaaagcatat gatttatgac
    53881 gagttgattg gagaaatcta ctgggtgatt ggcaagattc aggcagatcc gaaattggaa
    53941 gaagaactgt accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccaggtgatt
    54001 cgtatgtagt ggatgaggaa acggatgctc gaatggagct caatcaagtc atcggtgaat
    54061 tggaacgaat tgcggacctt gccaaagaac ctgatatccg ccaatacttg tttgagataa
    54121 aagcagagtt ggaaattgag ggtatcaccg cggagtaata gggaaataac ccactgatag
    54181 gcactgaccg cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgttcatgg
    54241 acggcggcag ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac
    54301 aaaaaacaag caagtaacca taaaaaatgc ttgttgtatc aaggatatta agtaatttgt
    54361 ataggtgtta actcatttgg aaaagttaac accttgaatt tggaggtgaa aacttggcgc
    54421 aacgaaattt agattatcgg ttattaaaag aacgacgcaa cgaatatggc gtttcacaaa
    54481 ataaattagc tactgcttgc ggacttagcc gcccatacct aaatcagata gaaaacggtg
    54541 gcgtaatcgc ctctacaaag accatgagaa aaatttttga tcaactggaa agtttcaatc
    54601 ccgatttacc tttaacgtta ctatttgact atgtaaggat tcgctttccc acaacggatg
    54661 tgcggaaaat tattcaagaa attctccatt tgaaattcga gtatatgctt catgaagatt
    54721 acgcctttta ctcctatcaa gaacaatatg tcatgggcga tattgtagtg atgttatctc
    54781 atgaagaaga taaaggtgtc cttttggaat taaaaggtcg tggttgccgg cagtttgaaa
    54841 cttttttact cgcccaaaag cgtagctggt acgacttttt tgaagattgt ctaaaagccg
    54901 gtggcgtgat gaaacgcttg gatttagcaa tcaatgatcg agtggggcta ctggatattc
    54961 ctgatttaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa
    55021 gttaccgttc cggggaactg ttgaaagctg atgaaaagga tggtatgggc aataccttat
    55081 atattggcag cctcaaaagc gaggtttatt tttgtttgta cgaaaaagat tatgaacaat
    55141 atatcaagtt agggattcca ttggaccaga ccgaaacgaa aaatcgtttt gagattcggt
    55201 tgaaaaatga ccgtgcttat tatgcgattc aagatttgtt aaaaggtcgt agcattgaaa
    55261 gtaccacgtt ttccattatc aatcggtatt tgcgatttgc ggataaagta gatggaaaac
    55321 ggcgaaccaa ttggcccttg aatgaacaat ggggtcgttt tattggacgg aatcggaaag
    55381 agattcaatt aacttctgaa ccgaaaccgt ataccattga acgaacatta aattggctag
    55441 gtagacaagt cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg aatcaaacaa
    55501 cgtatatcca ggatatggta cagaatgcgc gactctcgga ccggcataaa aagattttgg
    55561 agcaacaaag tatggcaatc gaaaacttga ttgtatgaaa agaggaattt tgatggaaat
    55621 tgtaaaagat atgttattgg tacttggtgg cagtatgctt ggtgttaccg tgatgtgctt
    55681 gatgaatgca agtgcagcag cggattgagc aatggaaaag aaaggaaagt gacaaaatga
    55741 atttcggaca aaatttatat caatggtttt taaccaatgc tcaatcttta gtcttactag
    55801 cgattgtggt cattggtttg tttttaggat ttaaacggga gttttccaaa ctgattggct
    55861 tcttggtgat tgctttgatt gcggtaggtc tggtctttaa tgcttctggt gtgaaggatg
    55921 tcctactcaa tttgtttaac cgaattgtcg gtgcctgagg gggaatcaca atggaacaga
    55981 tgcgtgttta tattgcgaac cttggaaaat acaatgaagg tgagttggtg ggggcatggt
    56041 ttaccccacc tgtcgatttt gatgaagtga aagaacagat tggattaaat gacgaatatg
    56101 aagagtacgc gattcatgat tatgaactgc cttttgagat tgatgagtat acgccaattg
    56161 aagagattaa tcggttgtgt aacttagcgg aggaactaga aggaacgcca attggtgaag
    56221 ctgcttctga gattcaacac gctttcttca attctttcga ggaaatggta gagcatgtgg
    56281 atgatattgt ctattatcca gattgcgatg atatgagtga tgtggcgcat tacttgattg
    56341 tagaagctgg tgattttggc gaagttccag aacaattgaa gccgtatatt gattttgatg
    56401 cttgcggtcg agatttggag attggaggaa attatttggt tactagtcgt ggagtttttg
    56461 agtatccaga atgaatcaaa cactatatat agtgtttgat tagtatatgt aagcaaaaaa
    56521 acacaatata tagagtgcaa actgaagggt aatgtgatat aatatttgcc gaagaaatat
    56581 ttttgaaatg aggtaatatt atggcaaata ttcaaacaag ttttattgat ttccataatt
    56641 caattagatt ggacgtagaa gacaatacgc tacttaagga ctataaagac caagtaattg
    56701 atggattaaa agattactta cctgatgatg tgaagtttga aactttttta cagggaagct
    56761 attctgttta taccggaata aaaagctgtg atgaaaaaat tgattttgac atcgatattg
    56821 cagttgcttt cgaaattgat catactgttt atgaagatcc aagggagcct aaactgtggg
    56881 taaaagaggc gctagttgaa atttttccta atgctcaagt taatttaaaa gtaccttgtg
    56941 taactgctac atttactgga aaaaagacaa aaaaaaatgt gcacgttgat gttgcggttt
    57001 atgctaaaga agatgaaaat tatttcttag caaaagcaaa agaattcagt gctcccgaaa
    57061 accgatgctg ggaggaagcg gatcccaaag tattaaaaga gaaaattaat agtcatgtag
    57121 cagattcaga tgatcgaaaa caatttcgaa gatgtattcg ctatttaaaa agatggaaag
    57181 ataacaattt taaccaagaa tataaaccaa caggaattgg acttacaatt aatgtgatgg
    57241 atacgttcct agttaataag tccactgatt ttttaactag aaaagttcag tataatgata
    57301 tggaatgtat gaagcagatt gtttcttcgc tgaaagattc atttgtgtat gagtacagtg
    57361 aaacagatgg ttggcattat cgtctacatg catatcttcc agtgaagcct aacagtgaca
    57421 cctattcaaa aatgacagtg aatcagatga gtgattttaa aaacaaactt tctaaattat
    57481 atgatgattt gattttcgct atcgatactg aagatgagta tgaagctaca aaacgattaa
    57541 ataatcaatt tggggaggat tttttaatta tttcagaaga agaagttaca gaaaaaaact
    57601 taagaaatgc atttgttaca gactatccga gtgcttaggt tggtgttaaa gattggatta
    57661 tactgatatt ttaaaagaaa agctaacaga tgttgtgcgt ttattcgatg gtaattacta
    57721 tggggatttt tcatttgcta caattggaaa gttgcgttta aaaattgaaa taccaaaaca
    57781 ttttccattg gtacttccta ggatttacat tcataatttt gatcatttag gactttacct
    57841 accacatgta gaaaaagatg ggttggtatg tttttcttca gatataaatt atttagtaga
    57901 tgaagaaaat tcagaattgg ttttattgca gtgtgttaat aaaactataa aaacaattga
    57961 ggaaggtttg aaagggttaa ataaaaatga ttttcgaaaa gagtttgttg ctttttggga
    58021 caaacaagag aatcaaattt cgttattcta cctttgtgaa ccagcgttgt ttatcaagtc
    58081 agttgataca ataatgtacg gtaagtcttt ggttgctttg gataaagaga acgaattaca
    58141 attaaatgca gcgagtagat tgtttaatgg agagaaagaa gatactcatg aattagatgc
    58201 atactatgtt ccaattagaa acaagaataa tattatgccc ccgaagaata ataaagcatg
    58261 gtcaaagaag gatattttac aaatagtaaa aaaaaatgta acaggtgcaa ataaaagatt
    58321 ttttttaaaa cttgaaaaaa gaaagaatag acataatgtt ttacttattc tagatatccc
    58381 aattgaagaa aagtcaaata atgtcataat tggatttctt tttacaaaac aatctggatc
    58441 tataaaagat tataagggta cgcctaggac tgtattagtt cagcgtcatg atgaattata
    58501 tttggtgaat agaacgagcg gagaaaacta tggattgggt gctaatatag ctattgttgg
    58561 ggtagggtca gttggcagta aaattgctga aaatcttggt ccattaggtg tgagaaagat
    58621 gacactcata gacaatgatt gttttcaagc tgaaaatttg tatcgaaatt cacttggtgc
    58681 agatagttta aaaaatgccc gatctcagaa taattttttt aaagttgatt taatgcagga
    58741 ggagctcgaa aaaaaattcc catacctttg tgttgaagtg gagtcaatga acgttttaga
    58801 cttaattagt atgcaacctg gttttttcaa ggaatatgac tatgtttttg tttgcatagg
    58861 agatacaatg gcaagcctgg ctttaaataa ttatttttcc aaaagtagtc aaaaagtttt
    58921 ctattcttgg ctggagccat atggcattgg gggacacact ctatttataa attattctct
    58981 aaatcaaaaa ggttgtttca attgtcttta tacagattca aactctaaaa ataggtgttc
    59041 aaatagagct tctctggtaa aagaaggaca aatttttgaa aggactttag cgagttgccg
    59101 ctcaaaattt ataccatata atgctattgc ctcgacggaa tgtgcaatta atgcaataaa
    59161 acttttccat aaagtcaatc aaaataatat gaccaatagt actctttttt catggatagg
    59221 agatgggagt acttttgaga atttaggata tcaatatagc gacagaaata cttttcttaa
    59281 tggagtcaat aagtttgaag aaagattctt ttctaaatat tgcaaagtat gtggggagtt
    59341 agatgattaa attttcgtgt gaccgtattg tcattgaagt aagggatgaa gttcttaaga
    59401 tatttgaaca atatcggcag ggaaaggatg acttgcctga agctggagga gttcttcatg
    59461 gaaaaagact taagaatggg agcataataa ttaccgatat aacaacacct caaataggag
    59521 atatacaaaa aagattactc tttaaaaaaa accgagaaat tcatcaacag ctttctaatg
    59581 agaaatggat agaatcaaat aagttttcta ttgcattggg agaatggcat actcatcctg
    59641 aagctgtacc aactccatca aatgttgata agaaaagctg gagactgaac gtgagcaagc
    59701 aacatgatga tagagtttat gtttttatta tcgttggatt ttctgatttg agaatgtggt
    59761 tactgtcaaa aaaatcagat attaggcagg ctataaaact ataaaggagt gattagttga
    59821 aatcacaaaa gaatatttgg tggtgggggt ttttagtagg aaaggtaatg ataccagccc
    59881 ttctcttaat ttctctaccg atgattactt ttttctttac caataaaaaa atgagtaaag
    59941 aagccgtgat ttttttcttt ggagaccaaa aagctacctt tattgaaacg ttagtaacat
    60001 ctcttcagct taatttgaat tacatttttt caattattat tgttatatcg ttattgaatt
    60061 tttttaaaaa aagaaattca ggaaaggtgt ttaactctaa tggaaatgtg tactacaact
    60121 atttttattt tgtattttgg gtagcagcaa ctttacttgg ttatgataaa attcagattg
    60181 caggaattcc tatacacatg caatataagt tggttttgag tggaattttt tctgaggtgt
    60241 tgccagatat atacgatgat cattacgaca gtgatggaac atgtaaagta agcattgaaa
    60301 aagaaaattt cgatgacatc gatggatatg actctgtgaa cttactgatt attgatactt
    60361 acgatataaa aatgagtgaa ttatctatgg aaaatcaaac atatcctacg ataattgtta
    60421 gagggaattc aatagatgga gttcgtaaag ttaatagatc tttaatcttg gaaataaaaa
    60481 aaacaatgga tgaaattcaa aaaagtgact ttaaaaaagt atttgttgct tcaacatcta
    60541 atccaaaaaa tagtatcaat attattaatt catcgtttag atttttcggt cgatcacgta
    60601 ggtttaaact ttatgttctt caaaaggatt atgcaagcaa tggaaagtat agtaagaaat
    60661 atagaatttt tatttaagaa ggaatttttc cttcttttta ttttggagga aactatgaaa
    60721 aaaataaaaa gctacaccag tatttgggca gttgaaaaag tcatttatgc gattaatgac
    60781 tttcagctgc cttttcctgt gacctttaac cagatggctt ggtttgtttt atccttgatg
    60841 tttgtcattg tatttgccca tgtgcctccg ctatccatga tagagggggc ttttttaaaa
    60901 tacttgggca ttccgatagc ggtcacttgg ttcatgtcgc aaaagacctt tgacggtaag
    60961 aaaccattgg gatttttacg atcctttatc agctatcact tacggtttaa ggtcactttt
    61021 gcaggaaaga aagttaaaga acagaagaaa cgatgggacg aaccaat
//
LOCUS       DACDVT010000007        59967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDVT010000007 DACDVT010000000
VERSION     DACDVT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.007"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            142..1140
                     /locus_tag="IWL52_000417"
     CDS             142..1140
                     /locus_tag="IWL52_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777006.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(1380..1463)
                     /locus_tag="IWL52_000418"
     tRNA            complement(1380..1463)
                     /locus_tag="IWL52_000418"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1427..1429),aa:Leu,seq:gag)
     gene            1846..3063
                     /locus_tag="IWL52_000419"
     CDS             1846..3063
                     /locus_tag="IWL52_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9777007.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3247..4083
                     /locus_tag="IWL52_000420"
     CDS             3247..4083
                     /locus_tag="IWL52_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9777008.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4159..5307
                     /gene="nagA"
                     /locus_tag="IWL52_000421"
     CDS             4159..5307
                     /gene="nagA"
                     /locus_tag="IWL52_000421"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9777009.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5528..6586
                     /locus_tag="IWL52_000422"
     CDS             5528..6586
                     /locus_tag="IWL52_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9777010.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6605..7624
                     /locus_tag="IWL52_000423"
     CDS             6605..7624
                     /locus_tag="IWL52_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9777011.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7726..8442)
                     /locus_tag="IWL52_000424"
     CDS             complement(7726..8442)
                     /locus_tag="IWL52_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9777012.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8411..9196)
                     /locus_tag="IWL52_000425"
     CDS             complement(8411..9196)
                     /locus_tag="IWL52_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9777013.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9187..9936)
                     /locus_tag="IWL52_000426"
     CDS             complement(9187..9936)
                     /locus_tag="IWL52_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9777014.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10234..10758
                     /locus_tag="IWL52_000427"
     CDS             10234..10758
                     /locus_tag="IWL52_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777015.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10774..12273
                     /locus_tag="IWL52_000428"
     CDS             10774..12273
                     /locus_tag="IWL52_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9777016.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12361..12963)
                     /locus_tag="IWL52_000429"
     CDS             complement(12361..12963)
                     /locus_tag="IWL52_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9777017.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13067..14023
                     /locus_tag="IWL52_000430"
     CDS             13067..14023
                     /locus_tag="IWL52_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9777018.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14328..15284)
                     /locus_tag="IWL52_000431"
     CDS             complement(14328..15284)
                     /locus_tag="IWL52_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9777019.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15458..16018
                     /locus_tag="IWL52_000432"
     CDS             15458..16018
                     /locus_tag="IWL52_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777020.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16011..16835
                     /locus_tag="IWL52_000433"
     CDS             16011..16835
                     /locus_tag="IWL52_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9777021.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16845..17462
                     /locus_tag="IWL52_000434"
     CDS             16845..17462
                     /locus_tag="IWL52_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9777022.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17480..18163
                     /locus_tag="IWL52_000435"
     CDS             17480..18163
                     /locus_tag="IWL52_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9777023.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18846..20129
                     /locus_tag="IWL52_000436"
     CDS             18846..20129
                     /locus_tag="IWL52_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9777024.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            20145..21032
                     /locus_tag="IWL52_000437"
     CDS             20145..21032
                     /locus_tag="IWL52_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9777025.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            21049..21885
                     /locus_tag="IWL52_000438"
     CDS             21049..21885
                     /locus_tag="IWL52_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9777026.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21888..22511
                     /locus_tag="IWL52_000439"
     CDS             21888..22511
                     /locus_tag="IWL52_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777027.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22508..23800
                     /locus_tag="IWL52_000440"
     CDS             22508..23800
                     /locus_tag="IWL52_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9777028.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24159..24256
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24372..24953
                     /locus_tag="IWL52_000441"
     CDS             24372..24953
                     /locus_tag="IWL52_000441"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9777029.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24956..26263
                     /locus_tag="IWL52_000442"
     CDS             24956..26263
                     /locus_tag="IWL52_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9777030.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26679..27167
                     /gene="purE"
                     /locus_tag="IWL52_000443"
     CDS             26679..27167
                     /gene="purE"
                     /locus_tag="IWL52_000443"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9777031.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27151..28284
                     /gene="purK"
                     /locus_tag="IWL52_000444"
     CDS             27151..28284
                     /gene="purK"
                     /locus_tag="IWL52_000444"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9777032.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28297..29592
                     /gene="purB"
                     /locus_tag="IWL52_000445"
     CDS             28297..29592
                     /gene="purB"
                     /locus_tag="IWL52_000445"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9777033.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            30119..31507
                     /gene="ltrA"
                     /locus_tag="IWL52_000446"
     CDS             30119..31507
                     /gene="ltrA"
                     /locus_tag="IWL52_000446"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9777034.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(31671..32048)
                     /locus_tag="IWL52_000447"
     CDS             complement(31671..32048)
                     /locus_tag="IWL52_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777035.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32304..33032
                     /locus_tag="IWL52_000448"
     CDS             32304..33032
                     /locus_tag="IWL52_000448"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9777036.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33032..33286
                     /gene="purS"
                     /locus_tag="IWL52_000449"
     CDS             33032..33286
                     /gene="purS"
                     /locus_tag="IWL52_000449"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9777037.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33288..33959
                     /gene="purQ"
                     /locus_tag="IWL52_000450"
     CDS             33288..33959
                     /gene="purQ"
                     /locus_tag="IWL52_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9777038.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            33960..36182
                     /gene="purL"
                     /locus_tag="IWL52_000451"
     CDS             33960..36182
                     /gene="purL"
                     /locus_tag="IWL52_000451"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9777039.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36167..37606
                     /locus_tag="IWL52_000452"
     CDS             36167..37606
                     /locus_tag="IWL52_000452"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9777040.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37638..38681
                     /locus_tag="IWL52_000453"
     CDS             37638..38681
                     /locus_tag="IWL52_000453"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9777041.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            38678..39256
                     /gene="purN"
                     /locus_tag="IWL52_000454"
     CDS             38678..39256
                     /gene="purN"
                     /locus_tag="IWL52_000454"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9777042.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39253..40788
                     /gene="purH"
                     /locus_tag="IWL52_000455"
     CDS             39253..40788
                     /gene="purH"
                     /locus_tag="IWL52_000455"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9777043.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            40804..42078
                     /gene="purD"
                     /locus_tag="IWL52_000456"
     CDS             40804..42078
                     /gene="purD"
                     /locus_tag="IWL52_000456"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9777044.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42200..43114
                     /locus_tag="IWL52_000457"
     CDS             42200..43114
                     /locus_tag="IWL52_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9777045.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43247..44812)
                     /locus_tag="IWL52_000458"
     CDS             complement(43247..44812)
                     /locus_tag="IWL52_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9777046.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(44805..46256)
                     /locus_tag="IWL52_000459"
     CDS             complement(44805..46256)
                     /locus_tag="IWL52_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9777047.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46270..47286)
                     /locus_tag="IWL52_000460"
     CDS             complement(46270..47286)
                     /locus_tag="IWL52_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9777048.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47506..48594
                     /locus_tag="IWL52_000461"
     CDS             47506..48594
                     /locus_tag="IWL52_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9777049.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48648..50174
                     /locus_tag="IWL52_000462"
     CDS             48648..50174
                     /locus_tag="IWL52_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9777050.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50176..51381
                     /locus_tag="IWL52_000463"
     CDS             50176..51381
                     /locus_tag="IWL52_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9777051.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51638..52615
                     /gene="guaC"
                     /locus_tag="IWL52_000464"
     CDS             51638..52615
                     /gene="guaC"
                     /locus_tag="IWL52_000464"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9777052.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            52879..54303
                     /locus_tag="IWL52_000465"
     CDS             52879..54303
                     /locus_tag="IWL52_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9777053.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            54764..55873
                     /gene="pdhA"
                     /locus_tag="IWL52_000466"
     CDS             54764..55873
                     /gene="pdhA"
                     /locus_tag="IWL52_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9777054.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            55876..56853
                     /locus_tag="IWL52_000467"
     CDS             55876..56853
                     /locus_tag="IWL52_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9777055.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            56878..58521
                     /locus_tag="IWL52_000468"
     CDS             56878..58521
                     /locus_tag="IWL52_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9777056.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            58529..59935
                     /gene="lpdA"
                     /locus_tag="IWL52_000469"
     CDS             58529..59935
                     /gene="lpdA"
                     /locus_tag="IWL52_000469"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9777057.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT        19278 a        10322 c        13203 g        17164 t
ORIGIN      
        1 tttactgagt aactaattat tttgcaggag gacaatttat ttacacaaaa ttattgacgc
       61 tcccagtatg tttttgagaa aataaatgtt aaaaatttat tattggtttg ctctatatac
      121 tgtttaatac cattgacagt aatgggggaa accggttgga gagcaaccac cttgaactat
      181 cagtggccag tagcatttag tttactaact ttttatccgt tttttcaact cttaagagga
      241 gaagaaataa atagaaaaat atattgggta agtattcctt tattgatatt tttaactaat
      301 caagaacaag tcaacgcctg tttttttgtt ctaacaagta tagttagttt atatttgatt
      361 gtcaatggac gatataatta taagttatca gtattttcaa taataagctt agcagaatta
      421 attttctctt tgacaactcc agggaatgca ttacgtgctg cacatgaaat taataaatgg
      481 tttcctgaat ataaaaattt caactttctt aataagttag atttaggtat ttcatcattt
      541 ggaaagccgt tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc
      601 cttctaacat ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt
      661 ttcttaaacc tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat
      721 ggaaataaaa gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta
      781 ggaacgaaac tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca
      841 ttattactat gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt
      901 tttttagtta ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc
      961 acagtatggg cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta
     1021 gtactaatgg cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg
     1081 caatttggat taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa
     1141 tatacaaaaa atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat
     1201 tgttttaaca cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc
     1261 aggctgttta catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg
     1321 cataaagaaa cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt
     1381 gcagatgatg ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg
     1441 tctgccaatt ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat
     1501 agttgtcaag ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt
     1561 caggactggg aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg
     1621 gttttatgct aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg
     1681 actatcatga caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc
     1741 ctaaataagt ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa
     1801 aatttggaaa gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa
     1861 aaatccaaca aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg
     1921 gagcggatgc aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct
     1981 tttggatttg aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca
     2041 gaaggctact cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc
     2101 caactgaaac attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc
     2161 gaattgatca cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca
     2221 atgccggact atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat
     2281 tatatttgcg atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata
     2341 acgagtcgga caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat
     2401 ccaaaagaat tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat
     2461 tccgacgaaa tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca
     2521 ttagcgcctg atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca
     2581 ggatttcgag taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata
     2641 gaagggctga atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt
     2701 atccaaaccg cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg
     2761 atctatgaac aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc
     2821 gtaaaaccaa aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt
     2881 ttggatgatg aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat
     2941 ggtggtggat ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg
     3001 gatgatttga aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa
     3061 tgattttaaa aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct
     3121 ttctagtgga gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga
     3181 agtttgcgac ttcctaaagt ataatacgaa tagaatgcga atacgcatat tttggggagg
     3241 atacttatgg aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa
     3301 caaaaaatga cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg
     3361 atcgaaaata atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta
     3421 ggggtaacga ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa
     3481 ttatttgaac tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta
     3541 ctaaaatctt ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat
     3601 tattatctcg gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat
     3661 ttgaaagaag cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa
     3721 atacaactga ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca
     3781 cgctactatt tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa
     3841 agtgaatttt cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc
     3901 gacgaagcgt tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac
     3961 tattacctca atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca
     4021 gaaagagcag cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg
     4081 taaatggtgt agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa
     4141 acaaagggag agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca
     4201 ggtgtcaaac atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa
     4261 gaactacctg agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt
     4321 ttggtagata cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa
     4381 ggcatcaaag tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca
     4441 acattgacgt caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat
     4501 caggaagcac caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa
     4561 gaacacaaag gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat
     4621 gaatggcaag aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa
     4681 ggtgtagaag agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac
     4741 agtaatgcta cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc
     4801 catgctttca atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta
     4861 ttatctttag aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat
     4921 gctgcgggaa tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt
     4981 atgatggccg gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta
     5041 aaagaaggaa cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa
     5101 gaagcaatta aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg
     5161 gctagtctga ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa
     5221 ggacgcgatg cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta
     5281 gatggcgtgg aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct
     5341 taagtttggt tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa
     5401 aagtttcttt aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt
     5461 taatacgtat tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag
     5521 gagaaagatg aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag
     5581 tgcaatcact tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat
     5641 tttttctgaa tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc
     5701 actggcattt cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa
     5761 tgtcttgatt tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt
     5821 cttaagctat aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac
     5881 gacctttttc ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc
     5941 ctcaattttc aatattggct accgaatctt tttgtattct tatggataca taaaaatgag
     6001 tggcttgaag atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc
     6061 tacttttgcc ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac
     6121 aggtgcagaa ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg
     6181 gctatttcag ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat
     6241 cggcgcaaca ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta
     6301 ttacagtcta gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt
     6361 gacggcaacg aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat
     6421 ggctacacca gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact
     6481 tttagcctcc aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg
     6541 gatcgttgta ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga
     6601 aagtatgaga atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt
     6661 gaatatttac cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt
     6721 gtcagcaaag gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa
     6781 tcttgcaaaa atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa
     6841 tcatatcgat ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta
     6901 ttcgccaaat gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac
     6961 tgcgtatacg acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag
     7021 tcgtgaaatc agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga
     7081 agctgttttg tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga
     7141 agctgctaaa aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat
     7201 tgtcagtatt catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt
     7261 tttagctcaa atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga
     7321 taatgaagct attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt
     7381 ttttgcaaat gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga
     7441 ccctgctatc cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg
     7501 ttcaaataca gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga
     7561 aataatagaa actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag
     7621 ataagaaaac atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt
     7681 tacttctgta tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt
     7741 gagctttttc cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa
     7801 atatttgatt ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac
     7861 ggatatacgt gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc
     7921 ttttagaata aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc
     7981 gatgaatgat ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt
     8041 ctgatcggct ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc
     8101 cagctgaaaa cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat
     8161 cacattcttt atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt
     8221 tccttgctgt ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc
     8281 aagcagcagc agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct
     8341 gaaactttcg attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc
     8401 aaccattgga tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg
     8461 gtaattctag atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac
     8521 cgtggcgttt gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg
     8581 tcgaaaaacc tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat
     8641 cgcagacggt cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct
     8701 gtccgtttct ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta
     8761 acacttgcaa aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc
     8821 tttctaacag catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac
     8881 cggcaattgc aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt
     8941 gatttagctg ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag
     9001 aaatgacatg aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt
     9061 gtttcgtccc cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg
     9121 cgactgctcc taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta
     9181 tcccatttaa atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa
     9241 acaaaacgtc atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc
     9301 tgtttggtag aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga
     9361 gatggctaag attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc
     9421 tgctatttct tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac
     9481 ggcgcctatg atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc
     9541 atctttgact tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat
     9601 ctcttgatca taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt
     9661 ttcagagaaa aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg
     9721 aagaccgatg taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac
     9781 gataaaatct aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt
     9841 cgggtcacca gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat
     9901 gctagagatt ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa
     9961 tatggcagaa acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt
    10021 ctggagcttt aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta
    10081 ttgtgaaaat tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct
    10141 tcctataaaa aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt
    10201 atgatagacg tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg
    10261 atttattggt atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga
    10321 gatgtattcg attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc
    10381 acaagcttat ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt
    10441 attgctagtg gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg
    10501 ccgatgaaac atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta
    10561 gctgttgtga tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt
    10621 accggttcca ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg
    10681 ggaacgaaag tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa
    10741 agcatgataa aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg
    10801 atcgcaatcg cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag
    10861 acagcaaaac cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg
    10921 cacattgatg aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg
    10981 ccagtttttc aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc
    11041 cctgaagtgt atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa
    11101 atcggttcat ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga
    11161 gaagaactgg aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt
    11221 tctatgactg tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa
    11281 gtcgcttctg tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag
    11341 gtcagagaca aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa
    11401 agagatgcag atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa
    11461 gcagaaaaag aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta
    11521 aaagaaaaag aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa
    11581 gcagaccaag cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa
    11641 atgcagatta aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca
    11701 cgtcgtgaaa agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct
    11761 aaagaacaag aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga
    11821 ttccgtgtgg aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca
    11881 gaagccgaag cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc
    11941 aatgccttca aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg
    12001 caattgatgc gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat
    12061 acaggaagca gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat
    12121 ttgctttctt ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc
    12181 gtagagaatt tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa
    12241 gaggcagaac aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa
    12301 aatggaggaa gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa
    12361 ttagtcctcg ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt
    12421 tcttagcaat cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt
    12481 ttctgtttca ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa
    12541 agtatctagt acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc
    12601 aatcagcaac aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga
    12661 cacgacgatc acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt
    12721 gtgaaaatag cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc
    12781 gatgatccta gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac
    12841 agcagataat gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca
    12901 taaaaaaaca gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg
    12961 catagagatt ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta
    13021 gtttttcatt attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat
    13081 gcgaacagtc aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc
    13141 gctgttgtat tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg
    13201 ctctacattg cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc
    13261 actgttcagt ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct
    13321 gttcatcccg cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt
    13381 ggtcactcat tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg
    13441 attaatggcg attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac
    13501 gaagcagctg aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca
    13561 aatggcagaa tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt
    13621 actgacattc attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct
    13681 cagacagccg ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat
    13741 taaagacatg ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat
    13801 ctatggttta ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat
    13861 tttaacacgt attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg
    13921 ccctgaattc gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat
    13981 tgaaatcaag gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct
    14041 aatattcagg tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa
    14101 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc
    14161 cttgtgactc ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc
    14221 tttcctctgc ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc
    14281 tgttcttatt acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc
    14341 catagaactt gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga
    14401 ttgggcaagt caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca
    14461 gatgtattcc cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga
    14521 tcagcacgcg ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact
    14581 tttggcattt ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc
    14641 aagatagtga cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact
    14701 acttcaggtt gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc
    14761 aacagagctc ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg
    14821 taactgccga aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt
    14881 gagcgtaact gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa
    14941 ttcttttctt ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct
    15001 ttttcacata gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt
    15061 ctatttggta cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag
    15121 cttccgtaag cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt
    15181 ctggaagcaa ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt
    15241 gcgatggtac caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc
    15301 atagttggat tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga
    15361 ggagaggact tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc
    15421 aaaacttaag tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct
    15481 tattatgagt gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt
    15541 gctgatcagt ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga
    15601 agcgatgcag aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat
    15661 tttgcgtcaa acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca
    15721 aatcaaaaca cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat
    15781 ccctttgaaa aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata
    15841 tgatgaatgg ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca
    15901 gatcgaagga gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta
    15961 cgttcatgag gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat
    16021 ttttaggatt tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg
    16081 atgatgattc gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa
    16141 ctgctagtaa agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa
    16201 aagatcgtac gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg
    16261 ttggtccaga tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa
    16321 gtaacggata cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg
    16381 ctgaagcagc tggatttcca ttagtgatgg tctcagctta ccggtctgta tcatcccaac
    16441 aacaagtttt cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg
    16501 aggcgacaaa gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag
    16561 gtctggcagt cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg
    16621 atgcaagtta cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg
    16681 gatttatcgt tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt
    16741 ggcacttacg ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc
    16801 tagaagaata tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt
    16861 ggaaaaagca aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga
    16921 tactcttagc ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc
    16981 aagaaaatga agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag
    17041 aactacaaga tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat
    17101 caaattgggg aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt
    17161 atggggatca gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa
    17221 ctatccaagg agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac
    17281 aactgttcgt ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca
    17341 ccaactatca ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt
    17401 atgcgcaaaa aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat
    17461 agcaaaggag aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag
    17521 aaaagatatc gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg
    17581 aaaattgatc cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc
    17641 gaatgcagta attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat
    17701 tgaggcagct gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg
    17761 ttcttcctac atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg
    17821 tcctacgcct ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag
    17881 tacggtaacg tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat
    17941 caatatcagt gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc
    18001 tgaacttcct atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca
    18061 agcaggagca aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa
    18121 aatggaaaaa tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag
    18181 acgaggaaga gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag
    18241 aattcaaaaa aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta
    18301 aaaccttttg ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt
    18361 ctatagaatg aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa
    18421 attgtcgggg cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt
    18481 gtgcatcgac gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt
    18541 tttagggaag agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca
    18601 gttgtctgat atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt
    18661 ttttagcgat gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc
    18721 atcctaggca gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta
    18781 ataaatatgt tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc
    18841 agagaatgaa gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga
    18901 cggcttgtgg gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc
    18961 aagtccgaca cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg
    19021 tgacgatcga ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag
    19081 cagcatcaga tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca
    19141 atcagattga accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag
    19201 atgaatccta tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg
    19261 tgaattcgtt tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac
    19321 cagcttcaaa ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat
    19381 taggaatcta tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac
    19441 cacaagttga gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat
    19501 ttgaaaatcc agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag
    19561 gggcccatcc agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg
    19621 ttagggatag agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt
    19681 caggggctcc tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg
    19741 gagaatctgg tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc
    19801 ataaagaaga agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa
    19861 ttttaaaagg agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg
    19921 cagatgatgc aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca
    19981 acggcgaagt gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc
    20041 ttttagttga aaaagtcatt tatgaagaag taacagcaga agaagcagca gagagtcttt
    20101 atgagtttgc gtcaaacatt ctaggatagc agcaggagga aggcatgaaa aaagaaaaac
    20161 taaaacggtt cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc
    20221 taggatttct attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg
    20281 attatcgttt gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga
    20341 gtgatgcccg ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac
    20401 ctgtgaaact gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa
    20461 cgactttctt tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg
    20521 ctctagtttg gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat
    20581 taggcgcacc tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt
    20641 tgatgtctgc ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga
    20701 tcccaggctc gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt
    20761 acaagatcac gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa
    20821 tcgcggcgtt tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg
    20881 ccacgaactt ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt
    20941 atgctagtgc tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt
    21001 tcaaatcatc aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca
    21061 gtaacgaaat ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg
    21121 gtcttatgga tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg
    21181 aatttgattc cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct
    21241 ggaatttcat tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata
    21301 gggacagttc tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga
    21361 aaaagattct ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg
    21421 attccgcagt ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt
    21481 ttaccacatt tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc
    21541 atccccaaag agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt
    21601 accaaaatta tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt
    21661 tattggaaat gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac
    21721 acggtatcga ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt
    21781 atgtttgcga tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat
    21841 aaatatcttg tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa
    21901 tagaagagat gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc
    21961 ttatcagtaa tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc
    22021 tgtctgctcg tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat
    22081 ctgtccatta cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt
    22141 gtttcatcat tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat
    22201 tagtgggaaa gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg
    22261 tatcccatag tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc
    22321 ttgtttattt gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt
    22381 ggtatttatg ccttttgtat ggactgttct ggtgtataca gcatgatttt ttactcactg
    22441 cattactgta tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg
    22501 actttaaatg aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta
    22561 tgaggcaatt gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc
    22621 tcaaacaagg atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt
    22681 aaagacatgt atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt
    22741 cattattacg tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg
    22801 atatgatgtt atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat
    22861 aacggtccaa aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc
    22921 gtatcacacg aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat
    22981 ccacttcgaa tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg
    23041 aaataagcac aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt
    23101 gaatttttgg ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta
    23161 tgggatgcat aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag
    23221 cgaactagct aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga
    23281 aatgtattta gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga
    23341 tgatatctct attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt
    23401 gacgtattcg ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag
    23461 caaaggacgg atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa
    23521 aaaggatgaa ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc
    23581 atatgaggta atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt
    23641 agatgatcta tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt
    23701 agatggtatt ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa
    23761 agcagtagtg attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta
    23821 cttcccgtag tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt
    23881 attcggaaaa aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt
    23941 ttttagatag gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt
    24001 aaaaatcatt agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt
    24061 gttagactgt tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc
    24121 acgtgcaaaa cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt
    24181 atatatcgtc ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag
    24241 taaaagattg gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta
    24301 acagaaacat agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag
    24361 gagcaaataa agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg
    24421 agggcgtatt aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac
    24481 aaatgggaaa acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca
    24541 ttgaagcttc tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga
    24601 tcttcgctag aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt
    24661 attcttttac gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag
    24721 aagacaaagt attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga
    24781 tcgaattatg ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt
    24841 ctttccaaga tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc
    24901 gtattgcttc cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga
    24961 aaaagaaaca caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta
    25021 tgcaggagca gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca
    25081 gatgacttat ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt
    25141 gacagttaac cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc
    25201 agtagctcca ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat
    25261 tatcgcttct gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact
    25321 attccctcct atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt
    25381 agcaatcgag aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa
    25441 tctgctactt gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg
    25501 gttcattcgt tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt
    25561 catgggaatg attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc
    25621 cttttatttt ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc
    25681 catggtcagc atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa
    25741 aaatgttgga gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt
    25801 aggcggtatt ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca
    25861 actttcggga atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt
    25921 aggattattc cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg
    25981 aggaatgtta gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt
    26041 tgatttcagc aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg
    26101 tttcaatgtt acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg
    26161 aaatggcatc gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt
    26221 gaaaaaagat gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt
    26281 tgtgaaatca gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta
    26341 ttttgccgtt tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga
    26401 ggtcgtgagc ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc
    26461 atgtttgttg aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca
    26521 taaaaaggaa tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga
    26581 acaataaaat gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt
    26641 gttaaaatat gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga
    26701 ttatggggag tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat
    26761 tcggtgtgtc ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg
    26821 aatatgcaca aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag
    26881 ccgcgcatct gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg
    26941 tccaatcacg aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag
    27001 gagtacctgt agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg
    27061 cgatacagat gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt
    27121 caatgttggc taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct
    27181 ggttcaacga tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca
    27241 aaagaaatgg gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa
    27301 gttgcagatt ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc
    27361 agaagaacag atgtcattac ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg
    27421 attttaccca tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc
    27481 ctgttagaaa aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc
    27541 gtcagtccga cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa
    27601 accactcgag gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta
    27661 gctcctgcca tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt
    27721 gaaaaagaga tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg
    27781 gtcgtggaaa acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt
    27841 gatcaggatg tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc
    27901 ctttcaggtg ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat
    27961 gaattagctc ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt
    28021 cagttcgatg cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg
    28081 ataagcaatg cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg
    28141 attgagaata agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt
    28201 cgcaagatgg gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc
    28261 aaacaaacga atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct
    28321 gaaatgggga atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt
    28381 ttagcagatg aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt
    28441 cgtgagcatg catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat
    28501 gatgtcgttg cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc
    28561 cattatggtc taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa
    28621 gcaaatgata ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca
    28681 aaagaacaca aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact
    28741 acttttggat tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt
    28801 gaacatgcgg caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca
    28861 aatatcccgc catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa
    28921 atctctactc aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt
    28981 attgctacaa gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca
    29041 cgtgaagtag aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa
    29101 aggaatccga tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg
    29161 gtcactgcct atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca
    29221 gaacgaatca tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc
    29281 aatatcgtta aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca
    29341 tttggtttga tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga
    29401 gaagaagctt atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag
    29461 ttccgcccgc tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata
    29521 gacgatgcct ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta
    29581 ggtttggaat aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac
    29641 caaaaaagaa gcagatccgt tagcggttct gcttctttta gttgtgcgcc ctgcatgggt
    29701 gaaaacttgg tggtgaaagt ccactacagg cttggcagta ggaactgtta gcgaaagaca
    29761 aggcggcaat cgtgaggttg tggctgaagg aagtcggacg caaactcctg aactgacgaa
    29821 cagaaactag atacaaggct gaattaggtc ggataagtct tcatcacaag acaaagtcca
    29881 atactgcccg aaacctatac agtaaatcta gcagttacat gggaggaaag ttttcactct
    29941 taccggggga gatctcatga gggtgttgtg ggcgttgaat gaaaacaaac cagcacaacg
    30001 atcatggcga catgatggtg aatcatgaga agtcagccga ggtcatagta gtaacgacac
    30061 tgaccgttac gaaggactga acaataagaa tcttgaataa tttaggaggt gtgagaagat
    30121 gcgacgaccg cagaaaacaa gaaaacttgg ctatcatcaa agagctagtg tggaacacga
    30181 agggtatgat gaagtgcgca gcacttcttg cggtgaacag aggaaacaag atggtgtaga
    30241 aaatctgttt gggagaattc tagatcggaa taatctcaat caagcgtatc ttcaagtagt
    30301 cagaaataaa ggggcggctg gtgcagatgg gatgaccgtc gatcaactgc ttccttactt
    30361 gaaagagcac cgcgaggaat tactatccaa tttacgccaa gggaactata aacctcagcc
    30421 tgttttgcga gtagaaatac cgaaaccaaa cggcggtgta agaaaactag gaattcccac
    30481 ggttattgat cgtatgcttc aacaggcaat caatcaagtg ttacaaccgc tatttgaacc
    30541 agaattctca gataatagtt atgggtttcg cccaaatcgc agcgcacatg acgcagtgaa
    30601 gaaagcaaaa tcttattatg aacaaggtta ccgatatgtg gtggacattg atatgaaagc
    30661 ctactttgac accgtaaatc acgataagct gatgtatttc attgagcgga aagtaaagga
    30721 caaacaagtc ctaaaactga tccgtcaata cttaatgagt ggtgtaatgg aaaatggact
    30781 catcacaaca actgaagaag gaacgcccca aggaggcaat ctctctcccc tgttaagtaa
    30841 catctacctg aacgaatttg atcaagtgct agaaaatcta ggacataaat ttgttcgata
    30901 cgccgatgac tgcaatatct atgttagaag caaacgagca ggacagcgtg tgatgaagaa
    30961 atccattcaa tttctcgaag aacaactaaa gttgacggta aatcgagaaa agagtgcggt
    31021 aggaagtcca ttgaaacgaa agtttcttgg attctgcatt ctgccaacaa agaaaggtat
    31081 taaaattcgc cctcacgcaa aagcaaaaca gcgtgtgaaa agcaagctca aacaactcac
    31141 taaaagaaac cgtggaagaa gtatccaact tattttaaaa gaaatcaagc aactaatgac
    31201 tggttggatc aactactatg gaattggtga gatgaaagaa ttcatgaggg agttaaacgg
    31261 ttggttgaag cgaagaatca gacaatatat ttggaaacaa tggaagaatc caagaaccaa
    31321 aagaaaaaat ctgattcgtt tagggatcga taaagcgaaa gcgtatgaat ggtcaaatac
    31381 aagaaaaggt atttggtcag tctccaaaag tcatattctt catcgatcat taacagacaa
    31441 agaactggcc cgccaaggat atgaggacat ctccttgaag taccagttcg tacactcaac
    31501 ttattgaacc gccgtatacg ggtccgtacg tacggtggtg tgagaggggc ggaaggtcag
    31561 ttggctttcc cctctactcg attattatat tttcttatca ataataggaa cctgatttta
    31621 aaagcggcta cctctttacc gtgcctcttt acatacaaaa aatagaggaa ttaagcgtaa
    31681 atggcttggc tattataact aaaatgctgg ttatggagat gttgcagcaa gttttcgaga
    31741 aaagcgtatt ccatctcttg tccattgaat aagatctgga tacgataatg atttttgaaa
    31801 cgcttctgtt catgaatctg actgattgaa agttcgcctt tgtgttctgt ctgataaaga
    31861 aaagtccttt tacggcattc agcacagcgc ttggttgctc ggcggatttt tcggaatgta
    31921 taaaaactag tatctttatt gcataaataa acgtctttga tcgacagcaa atcattatat
    31981 agacaggaac gttcttcagc tgttaattct gataaaaaaa gctgaccttg ttttattgca
    32041 tcttccatcg gcaagtgcct cctcgaaccc attatccaaa tttttagtta attctaatat
    32101 accatcatta agtataaatg taaacgcttg ttgatggtat aagattttcg tttctgataa
    32161 aacacgaaca ttaaaagaat ctaatgcctt aaatgtacat atttttgttg atttataaaa
    32221 agatgattgc taaaatttaa agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat
    32281 gtaaataaaa aggagagacg tacatggaga agaaagaact tttgtatgaa ggaaaagcca
    32341 aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta gatcaagcaa
    32401 ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg aataatcaaa
    32461 tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat tttattgaac
    32521 aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg gaagtggtta
    32581 tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat
    32641 tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat ccatttatca
    32701 atgaagagca tatctatttc ttacaacttg caaagccgga agaaatcagt gagctgaaag
    32761 cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc ggtttggttt
    32821 tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt gcggatgaag
    32881 taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg gacaaagacg
    32941 tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat aaccgtctat
    33001 cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat atgtaaccta
    33061 taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg
    33121 atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac ttgctgatgc
    33181 agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg caaatgtaaa
    33241 tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg
    33301 tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt cgcgagatca
    33361 tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga tttgatgggg
    33421 ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg attgctcggt
    33481 tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca gtattcggta
    33541 catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta ctgagacgaa
    33601 atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc aagacggcct
    33661 ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat ggggaaggaa
    33721 attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag attgccttta
    33781 cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg aacaaagcag
    33841 ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg ctaggatcag
    33901 aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg accgtataga
    33961 tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc tatgccgaat
    34021 ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga agaatgccta
    34081 attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc tataaaaatt
    34141 ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa ggccctggag
    34201 aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc
    34261 acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt ggcggaatca
    34321 tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca ttgaggttcg
    34381 gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca ggtatcggcg
    34441 gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt gatccttgtt
    34501 atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac aaacatatcc
    34561 agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc
    34621 gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa gaagaacagc
    34681 aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt
    34741 tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg ggagcagcag
    34801 gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt atattgacat
    34861 tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg ttatcggaat
    34921 cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc caagaactat
    34981 ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac gggcaatatc
    35041 ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta gctgaagatg
    35101 caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt cagcagttag
    35161 ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg ctgcagcaac
    35221 cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta caaaccaata
    35281 cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg aataaagcgt
    35341 tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa atcggaggac
    35401 aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag cccttggcga
    35461 tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg gaattatcga
    35521 cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa
    35581 atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca atgattggta
    35641 tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa aaatcagggg
    35701 atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa ttacaaaaga
    35761 tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt gaaaagagaa
    35821 accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct catgactgtt
    35881 cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag ctagggatat
    35941 ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca agatttgtgt
    36001 tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat gctgctgttt
    36061 tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac ggtcagatca
    36121 aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt cttatgaagt
    36181 gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag acgccgctag
    36241 tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg caggtatcgt
    36301 gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt ctgaggtatt
    36361 caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac atgtacgtta
    36421 tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca aatttcatga
    36481 tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc ttagaaaaga
    36541 gttagaggaa aatggggcga tcttccattc taactccgat acggagatat tgatgcattt
    36601 gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt tgaatgaagt
    36661 caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg ctttggaccc
    36721 aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg tgattgcctc
    36781 ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc agcctggtga
    36841 gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg acacgcagct
    36901 ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca tcgcagggat
    36961 caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat cgccagtaaa
    37021 agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg gttatgcgga
    37081 agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg cccgaacatt
    37141 tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat ctgctgtacg
    37201 aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc ggggtacgac
    37261 aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc atgtgaggat
    37321 tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa cgcgtaaaga
    37381 actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg cagattcttt
    37441 aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag atgctccgta
    37501 ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat atgactatga
    37561 agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa
    37621 gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg
    37681 gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga
    37741 tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc
    37801 cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca atcgaagcag
    37861 acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat atcttagcac
    37921 aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat gatccgttac
    37981 gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat atggcgctga
    38041 ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat gatctagccg
    38101 gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg atcaacccag
    38161 gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac tctcttgttc
    38221 gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca gaattgaaaa
    38281 aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc
    38341 tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg tttgtagaaa
    38401 atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc tcatggccga
    38461 ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg gagatgtatg
    38521 aaatcttcaa tatgggcatc ggtatggtcc tggcagtcga agaatcgaaa gtaaaagaaa
    38581 tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca
    38641 aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg tttttgcttc
    38701 tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag gattggaatc
    38761 ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc
    38821 gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa aagagtatga
    38881 agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat tagctggata
    38941 tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca tcaatatcca
    39001 tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct ttgaagctgg
    39061 agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata ctggaccaat
    39121 catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt tagaagaaaa
    39181 aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg
    39241 aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg gattgtagca
    39301 ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg aacaaagcat
    39361 gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa
    39421 atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct tttaggaaga
    39481 cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc aatcgacttt
    39541 gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt cgaaatagca
    39601 gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac
    39661 catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact ttcagaatta
    39721 cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa agtctttcgt
    39781 catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt ggaagaaaaa
    39841 gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat
    39901 agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc gatcgcagca
    39961 gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc agatgctgcg
    40021 ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca catgaatcct
    40081 tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta tgaagcagat
    40141 cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct gccaacagca
    40201 gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac acaagaagct
    40261 ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt tgcgcaggaa
    40321 gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt acaagatcaa
    40381 gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa gccgactcca
    40441 gcagaagcaa aagcattagc gtttgcttgg agagctgtca aacatgtgaa atctaatgcg
    40501 atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat gaatcgagtc
    40561 ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc agttttagct
    40621 agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca tggtataaaa
    40681 gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat ggcgaataaa
    40741 tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag aggagtcgga
    40801 taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt
    40861 ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa
    40921 gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat ccattttgtt
    40981 aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc
    41041 gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc agcagctctg
    41101 atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat cccgacagct
    41161 aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg
    41221 ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt ggctaccaca
    41281 gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt tggggatagt
    41341 agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct tttggcgttt
    41401 gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg ggcatttgat
    41461 aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc gcaaatttca
    41521 aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa
    41581 gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac gaaggaagga
    41641 ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct
    41701 cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg tacagtagag
    41761 ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc cggttacccc
    41821 gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct ttctgtttac
    41881 tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg tctttattta
    41941 gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg
    42001 gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc tgccaatagc
    42061 gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca aacataatcg
    42121 gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt gttgtgtata
    42181 taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc agaaagcagc
    42241 tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa aagtgattga
    42301 gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg gaaacggtga
    42361 atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag aagatctttt
    42421 tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta aaaaacatat
    42481 cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg atgctttact
    42541 tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt ttgatgaatt
    42601 ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga
    42661 attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat ttggcttgaa
    42721 acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag caagtatcga
    42781 tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc aagcatggtc
    42841 accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt
    42901 aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg gcttggcttc
    42961 tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga tgaacgtagg
    43021 gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag attggtacga
    43081 attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag gtcaaaaggg
    43141 ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga tattctgtct
    43201 ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag ttatttttgt
    43261 ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt ttcttaaata
    43321 aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct gaaatacgat
    43381 agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc agataatcga
    43441 tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga cttaatgcaa
    43501 cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct ttgataaaat
    43561 ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag ggcagagcag
    43621 ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg
    43681 ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc ttttctttca
    43741 ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt gttcgagttg
    43801 gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt ttcgataatg
    43861 aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt ttcgcttctt
    43921 gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt tcaagtatca
    43981 tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg
    44041 cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt tgggctacct
    44101 gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc
    44161 gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac ttcaaataat
    44221 cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct aataatacgg
    44281 tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat gtttcactga
    44341 tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa
    44401 gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact gctttttcta
    44461 ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct gcctgtacct
    44521 ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg agacgagtcc
    44581 aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga
    44641 tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc atctcaccat
    44701 ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt atttgttccc
    44761 gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac atgtttgttt
    44821 cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc ttctcggtat
    44881 ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag acgcttgttt
    44941 acgttgtaaa gtccgtagcc gatactgaaa tctgaatcga tccctttggc taactcttta
    45001 cgtatttctg ccagtttttc tgcagggatg ccaatcccgt tgtctataac tttgaattgg
    45061 atcatctcat caattttgca tgtggtaata cgaataaatc ctttgtgacg gacgtacttc
    45121 gttccatggt atacagcatt ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca
    45181 ggaaaaagtg tttctggtat atcgatctta tagtcaagca tatggccgta tcttattttt
    45241 aagatgacta gataatcatt gatatggcta atttcttttt caagaggaat ccagtcttgt
    45301 cctttgctta aagacgtacg aaaaaagccg gaaagtgcat gggtcatagt cagagcttct
    45361 tcaaagcagt cttgttcgat caaagaaata atggcatcca aactgttata aataaaatgg
    45421 ggagtaatct gagcttgcaa tacacgaact tcactttgtg ctaaatggta ttgtttcaaa
    45481 gcgttctccc gtaaagactg gtttagatct tccgccattt tattcatgct agcggtaagt
    45541 gtttgtaatt ccagtgtttc cggcagggtt aaacgaaagc caaggttccc tgaagaaagt
    45601 tgttctgaca tctcgatcag ctgattgatc ggttcttgga cctttctctc taaaaagcga
    45661 cgattctttt tcgcatagaa aaaaatggta ctaagaactc caagttccac tattgttaat
    45721 agaatgagcg agcggacaag tgtcgtattt tttttgcttg ctaagtcgat ctccacacga
    45781 acaaaatctt gtaagatatc tgacagcagt tgagtgacag aatctacctg attgatgatc
    45841 tcttcattgg tatccataaa atcttttgcc aagatattat tgatcagctc gttttgataa
    45901 tcttccagtg tatcgatcgt acgtagcgat acatctaacg tactaatttc tttaagtgta
    45961 tttgtattat ttttgatctc tcgaatatca ttcctaagtt cttctaggat attttcctca
    46021 ttgaactcat ctacggttac ttggccagaa atcaagtccc acatactttc caaaactttt
    46081 tgatcaacct tcaaagcaat tgcatctgct ttttggatat ttgcgatcgt ctgttgataa
    46141 atcaaaagct gttgcgaata taacagtatg ctcagtagca aagggataac agcaaataga
    46201 agcatggctt tgtttgcttg ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata
    46261 cttctccagt caataatttc tggttgggat gttagctata ttctgctggg aaaagacagt
    46321 ctcggaaata aaaatatctt taggtaatgt ttttcctgtt tgaatatttc caaaataacg
    46381 agtaacggta ttggctacat accagccgac atcaggttta cactcgacta cacaattgac
    46441 gattcctgag cgtagcttct cgatcatatc ttcttgtcca tcaatagtta caatgatcaa
    46501 atcttctcca ggtttgattg atgtttcttt tatcgcagct aaagcaccgt gagtcatttc
    46561 atcactatgg ctgtataata catcgatttc ggataaatca tttgtttcta aatatcgttt
    46621 catctggtct tttcctttca tccgaataaa atcaccgaca attcggtgaa caatagtcat
    46681 cggaggttta tggtcgatgg ttccatcttt ttttagggtt ggtttacgat tgattgattc
    46741 caaaaatcct tcggagcgta gcttggtcgg tgtggagttc tctgaaccag caagttctag
    46801 gattctgatt tcctgtttct ttgaatttgt gaagtgattg ctgacaaata aacccgcccg
    46861 atttccttct gctttgaaac tggggccaat gtgggtgacg aaaagctcgg gatcattcgt
    46921 ttgaatatgt ctgtcgacaa gtatgacagg gatccctgct cgctttgctt cttttaatac
    46981 agagtcccac ccggattctt gcaaaggagt aaaaatgatc atatccactt tataggcaat
    47041 gaacgtccgt atatcttgga tttgccgttc ctgattcatg tagccatttc ggtagagaac
    47101 ttggtaatct tctttttcaa gcgcagtgat aattgattca gtatgctttt tccgccagct
    47161 actctccgtt cctgattgcg aaaaaccaac gactagtggg tcaggtgctt cctttgtatt
    47221 aggtgtacac cccatcattt ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg
    47281 tttcacttta aatcactcca tctggatttg ttagtactat cttatcagat aaaagggaaa
    47341 atcaaagtca aaaggaatca atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa
    47401 aaaaagtaca cctaaaaaat ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc
    47461 tttattagct atactcggtg tatcaattga aatgggggat aaaaaatgaa aaacaaattt
    47521 agaaagattg ttgggactat cgctattttg accgcaagca ttctattaag tgcgtgtggc
    47581 aattcaggat ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca
    47641 gcagaaagat ggatcgctga tgggaacaac atggtctcgg aattagaaaa actgggttat
    47701 aaaacagatc ttcaatatgg agaagataaa gtagaaaatc aagtagcaca aatagaaaat
    47761 atgattacaa aaggggtaga tacacttgtg attgcttcta tcgatggttc ggctttaact
    47821 gatgtgttag ctaaagcaaa agaagcagat atcaaggtga tcgcctatga tcgtttactg
    47881 atgaattcag aaaatgtcga ttattacgcg acttttgata attttggagt aggtgtatcg
    47941 caagcagctt atattgaaga gcacttagga ttgaaagaag gaaaaggacc attcacgatt
    48001 gaattatttg gtggctcgcc tgacgacaac aacgcactaa tcaattacaa cggagtgatg
    48061 tctgttttac aaccatatat ggacaacggt cagttggtcg tgccatctgg acagacgagt
    48121 ttcagtcaaa tcgctacgct tcgctgggat ggttctacgg cgcaagcaag gatggataat
    48181 ctgttaagcg caaattatac tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca
    48241 ataagtttag gaatcatttc gtcattaaaa ggcgtggggt atgggagtga aagtaaacct
    48301 ttacctgtga ttactggaca agatgcgaca gttgctggcg ttaaatcgat cattgccggg
    48361 gaacaaacac aaacgatctt taaagacaca cgtatcttag caaaaaatac gattgaaatg
    48421 atcaaagcaa tcagtgatgg agaagaggtt ccggtcaatg atactgaaac ttatgataat
    48481 ggggtgaaaa ctgtccctac gtatttagca aatacagtat ctgtcgataa agataattat
    48541 caagcagagc tgatcgacac cgattattat aaagaatccg atctgaaaaa ttaagaggaa
    48601 ccaaacaagg aactaaagag atctaagtca gagagaggat aagaattatg gcggattgta
    48661 ttttaaaaat ggcagatatc accaaaaaat tttctggaat ccgagcgtta gattgcgtga
    48721 atctgatgat tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat
    48781 caacattgat gaatgtcttg agcggcttgt atccatatgg ctcctatgac ggggaaatct
    48841 tttatgatgg aaagctgtgt caatttaaag atttgaaaga tagtgaagaa aaaggcatcg
    48901 tcatcattca tcaagaatta gcactaagtc cttatctttc aataaaagag aatatttttc
    48961 tcggaaatga acaagcagaa agaggcgtga ttgattggga actgacagaa aagaaaacat
    49021 atgaactgct gaataaagtc ggtttgtcta tcgacccgaa tcttctagta tcccagattg
    49081 gcgtagggca acagcaacta gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt
    49141 tgattttgga tgagcctacc gctgcattga atgaagaaga aagcgcgaat ttgctgagat
    49201 tgatccgaga gttgaaagag caaggtgtaa cctccatcat tatttcacat aagctgaatg
    49261 aaatcgtgga tatcgcagat cgaatcacca ttttacggga tggacagacg attgagacac
    49321 tagaaaatga aatgatcagc gaagagcata ttattcgcgg aatggtgggc agagatttag
    49381 ttgatcgtta tcctgaaaga aaaccggata ttggagaagt ttattttaaa gtagaagact
    49441 ggacggtcta tcaccctctt gatcacgagc gaatcataaa cgaccacatc aacttgcatt
    49501 tgaaaaaagg agagattgta ggaatagctg gactgatggg agcgggcaga actgaattcg
    49561 ctatgagtct atttggacat tcttatggaa gcaagatatc cggacgtata ttaaaggaag
    49621 gggcagagat atcagttaag accgttcctt cagccattgc aaacggactc gcttatgtct
    49681 cggaggaccg aaaatcttta ggactgaatc tgctgatgga tattcgggaa aacgtgacgc
    49741 tgagcagttt ggataaaata agtcaaaaag gagtattaga taaagaaaaa gaagtaactg
    49801 aggcagaaaa tttccggaaa aagatgcgta taaaaacaaa ctccatccat caaattgtca
    49861 gtagtcttag tggtgggaat caacaaaaag tcgttttatc caagtggctg atgacacaac
    49921 ccgatattct ttttttggat gagccaaccc gtgggataga tgttggggca aaatatgaga
    49981 tctacacgat tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt atctcttctg
    50041 aacttcctga aatattaggg atgtgtgatc gaatctatac gatgaacgaa ggacgtatta
    50101 caggagaagt ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta atgacgaaag
    50161 aagaggtggg ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta
    50221 aatttgaaaa gtagagcagc tgatcttttt ggtcgctaca gcatggtgat cattttagcg
    50281 ggactactta ttgcgtttca attgatgacg gatggtattt tctggcgtcc attgaatata
    50341 actaaccttg ttctgcaaaa tagccatata ttagtactag ctgcagggat gttattagtt
    50401 gtgctcttag gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt
    50461 gcgggcatga tgatggtcaa tcgccaaatg aacccgtgga tgacagttat cgcatgtctt
    50521 gcagtaggcg ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc agggataccg
    50581 gcgtttatcg tgactttagc aggattgctg atgtttcgtg gactgacaca agttgttcta
    50641 ggaggccaat ctctagcacc ttttccagct gttttccaaa aaatgtctac gggttattta
    50701 ccagacttaa gcgaaggaag cagccttcac ttcttgacat tgattttagg aatgatcatc
    50761 acgatcgtgt tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa tcatttattt
    50821 gaagtggaat ctccaacagc tttcttggta aaggcggtat ttacagcagt cgttgtacta
    50881 ggaataactt atatctttgc ctcttatcaa gggtatccga ttattttgat cattcttggt
    50941 ttgatcgttg cagcatatgg gtttttgaca aataaaacag tagcaggtcg acagatttat
    51001 gcgacgggcg gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg
    51061 ttctgggtat tcgtcaacat gggagtcatg gcagctttag ctggattagt tttagctgct
    51121 cgattgaatg cggcaacacc tcaagcaggt acatcgatgg aactggatgc aatggcggca
    51181 gtatattttg gtggtgcttc gacttccggc ggcattggaa caatcatggg tgcgattgtc
    51241 ggtggcttgg tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt cggggtagac
    51301 tggcagcagg caatcaaagg attgatcttg cttttagccg ttgtgctaga cttgtataat
    51361 aagaaaaaac gaacaacgta ggagaacgcc ggttattcag aaaaaagact gtgacaagac
    51421 atttgtcaca gtcttttttt cgctaaaagc gaacattcat tgtttcgttt gtatagacta
    51481 aaaataatat atgttttttt accattcgta taaattctct ttttgctctt gatttttttg
    51541 cttaattgag ttaaattaca tagggcgcta aaaaaagcct tgattaaatt aataatgttc
    51601 gtgttttgct ggatgtgaac atcaggagga taatgtaatg aaagtatttg attacgaaga
    51661 tattcaatta attcctaata aatgtatcgt aaatagccgt tctgaatgtg atacaacggt
    51721 cacattaggt aaacatacat tcaaaatgcc agtagtgcct gccaacatgc agacgattat
    51781 tgatgaaaca atcgcagaat tcttagcgga aaatggttat ttctatatca tgcatcggtt
    51841 tgatgaagca gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc
    51901 gatcagtgtt ggcgtcaaga aagaggaata ttcctttata gagaagttgg cagaagaatc
    51961 gctaaatcct gattatatca ctatcgacat cgcgcatgga catgctaact cagttattga
    52021 tatgattcag catattaaaa aatatttgcc tgaaactttt gtgattgccg gtaatgttgg
    52081 tacaccggaa gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat
    52141 tggtccagga aaagtatgta ttacaaaaat caagacaggc tttgggactg gaggctggca
    52201 actggcagct cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg
    52261 gatcagaaca catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat
    52321 tggttcattg tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat
    52381 cgtgtataaa gaatactttg gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt
    52441 tgaaggaaag aaaatctgga tccaacacaa aggatctttg aaagatacac tagtggaaat
    52501 gcaacaagac cttcaatcat ctatttctta tgctggagga agagatctgg aagccattcg
    52561 aaaagttgac tatgtcatcg tgaaaaattc gatttttaat ggagacacaa tctaaaaaat
    52621 aaataaaata ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa
    52681 acaaaaatag ttctttatat gttttgtttg gctggaatta ttatcgcaga tacaagcttt
    52741 tgaacatcat tattcagaat aagggactgt gataagattt ttgtcacagt cccttatgtt
    52801 ttgcaaaaaa agtttttctt ttttctcgta aattcggtaa aatcaaaaga aaaaatagac
    52861 aatagaaaag agaagcttat gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt
    52921 caaattatgg aacagattat gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa
    52981 cttgaatcag aacgaaaact agggcaactt tttggtgtga atcgttccac cgttgtccat
    53041 gcattggaag agcttaggga gcttggggta ctaacgagca aacgtggcag cggatggttt
    53101 gttaatcaga ctgaatgggg gaaattcgct gttccaagaa tcgattggcg gcaaatgatc
    53161 tctcctcgtt atgagcagac ggactggtat gagcaaaaaa taagagaagc aaagcagatc
    53221 caaaaaacat cgtttcttga tttatacgct agcgagatgc cagatgagct tttgccgaat
    53281 tttgagtttc cttcttattc tcttgaagaa atcttagcgg aagaaaaaga aatggatctt
    53341 ttaggctata ctccgttacg taacaaaatc aaacggtatt tggaaagtaa atttgaattg
    53401 gcattttccc ccaaccagct tcttgtgact ggtggcggtc agcaagcaat ctttttgatt
    53461 ttacaaacga ttctttcagc tggagagggg atagctgtag agtcaccttc ttttttttac
    53521 cgattacctc ttttcaaagc ttcaggaacg cgtttatttg ggataccgat ggatgaagaa
    53581 gggatcgact tagaaaaact gaaagaatcg atccataaaa ataaattgaa agcagtgctt
    53641 atcaacccga attttcaaaa ccctacagga aaagtgatga gtcaaaaacg acgagaaaag
    53701 ctagtagaat tgtgtcgaca gtatcggctt ccaattattg aagacgatgt atttacggat
    53761 ctttcatttc taaaccaaga agaagtcctt cctatacgaa cgattgatcc ggataatgtg
    53821 ttatacgtcg gttcgttatc tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata
    53881 ggacctgaga cttttatctc gcaactagct caagcgcaag aaatgatgga gttttcgatg
    53941 aatatcctta cacaaatcac agtggctaat gtatttaatg aagcaattga tttgaaaatg
    54001 caagatgtaa gaaaaaagtt gaaagaaaaa agccgactga taagcgcttg gggagattca
    54061 caatcgtttt tcaagcttca ttcaatttta ggtggttact atgcctggat tacttgggat
    54121 gggaaaaaac tagaccccga actagcagaa aaagtagtag atatgggtct tggaattgca
    54181 ccaagtctac tttttgggca agatataaat ggaatcagaa tcaattacag taggctggat
    54241 gaaaaccaaa taccgttgtt ttattcaaag atgaaaattc tagaaaagtg gttaacagaa
    54301 taatttcaca aagtaaatag attaaagaat ataacagctt ggatttaaat ttcaagctgt
    54361 ttcttttttt tcggaaaaag aatacgatat aaagaaacta agaaaaaacc attatttgta
    54421 cgtccgttca cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat aacaccaacg
    54481 tttttaaata gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa
    54541 atataaaacg ctttccttat tgtgatttta ttcactaatg ctgttaatat gcggattggc
    54601 ataatttcct ttcatcgtga aaatttgtgc ttttagcttg tatttctcat aaatccatga
    54661 taatttatta atgaagacgg ttaaatagtg atacagtttt attcactgtt tcaatcgcag
    54721 aatgaagaga ttttcttata gatgagagag gaatgtgtga caaatggcaa acaaaaaagc
    54781 accgatcgac ttccaagcac tgctagatga agtcaatgca gatttcccag tatatcaagc
    54841 acttgatcaa gatggaaacg tagttaacaa agatttagta cctgatttat ccgatgatga
    54901 actcgttgaa ttaatgactc atatggtatg gtctcgtgta ttagaccaac gttcaacagc
    54961 gctgaaccgc caaggacgcc taggcttttt tgcaccaact gctggacaag aggcaagtca
    55021 gctggctagt gcttacgcat ttgataaaga agatgttctt cttccaggtt atcgggatgt
    55081 acctcaacta gtaaaacatg gtttaccttt atctcaagct tttttatggt ctcgaggaca
    55141 tgctgcaggt aattcttatc cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg
    55201 tgcacaatat gtacaagcag ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa
    55261 tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat
    55321 caatttcgct ggcgcttacc atgcaaatgc cgtattttat atccaaaaca acgggtatgc
    55381 gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc
    55441 agctgggatt cctggtattc aagtcgatgg aatggatccg cttgcagtat atactgtatc
    55501 taaaatggca cgggaatggg cgatcagtgg aaatggacca gtcctaatcg aaacactgac
    55561 ttatcgttat ggaccacata cactatctgg tgacgatcca acccgctacc gtacaaaaga
    55621 cacagatgac gattggcaga aaaaagatcc attgatccgc ttccgtaaat atttgacgga
    55681 aaaaggactt tggtctgaag aaaaagaaga acaagtcatc gaagcaacta aagaagaaat
    55741 caaagcagct attgctgaag ccgacaaagt accaaaacaa aaggtttcag atttcttgaa
    55801 aaacatgttc gaagtatctc cacaatcaat caaagaacaa attgcaattt atgaagcgaa
    55861 ggagtcgaaa taatcatggc acaaaaaaca atgatccaag caatcacaga tgccttagca
    55921 cttgaactag aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga
    55981 ggagttttcc gggcaactga aggattgcag gaaaagttcg gagaagaccg agtattcgat
    56041 actcctttag ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt
    56101 cgtccagttc ctgaaatcca attctttggt tttattttcg aagcaatgga cgaagtagta
    56161 gctcaaatgg ctcgtacaag atatcgaatg agcggcacta gaaatctacc catcacaatt
    56221 cgttcgccat ttggtggcgg tgttcataca ccagaattgc attcggataa tttagaaggt
    56281 ttgatcgctc agtctcctgg tattcgagta gtgattcctt ccaaccctta tgatgcaaaa
    56341 ggactattga tttcttctat tagaagtaac gatccggttg ttttcctaga acacatgaaa
    56401 ctatatcgct cattccgaga agaagtgccg gatgaagctt acgaagttcc tttggataaa
    56461 gcagcagtta ctcgtgaggg aacagatgta tctattatca catacggagc aatggtgcgt
    56521 gaagcaatca aagcagcaga caatcttgca aaagaaaata tatctgttga aatcattgat
    56581 cttcgtacag ttgcgccatt agatgtagaa acgatcattc aatccgtgga aaaaactggt
    56641 cgcgtagttg ttgtccaaga agcacaacga caagcgggcg ttgccgcgca agtagtttct
    56701 gagatctcag aacgcgctat cctttcatta gaagccccaa tcggacgagt ttctgcacct
    56761 gatacagtct tcccatttgg acaagcagaa aatgtctggt tgccaaatgc aaaagatatc
    56821 gaagacaaag taaaagaaat cgcggaattt taagaaaaga agggacgaaa caaagtcatg
    56881 gcttatcaat ttaaattgcc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
    56941 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
    57001 gacaaatcag tagaagaaat cccgtcacct gttacaggaa cggttaaaaa tgtgattgtt
    57061 ccagaaggta ccgtagcaaa tgttggggat gtattagtag aaatcgatgc accaggacac
    57121 gaagacaatg aaggggacag tggtgtagcc gcagaatccc aaactccagc taaaccggct
    57181 gctgaaccca ctgttgatac agaatcagct ggatcgtcaa gcgaaggagt cttccaattt
    57241 aagttaccgg atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa
    57301 ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga caaatcagta
    57361 gaagaaattc cgtcacctgt cacagggaca gttaaaaacg tgattgttcc agaaggcact
    57421 gtagcaaatg ttggggatgt attagtagaa atcgatgctc ctggacacaa tagtgcaccg
    57481 tcaactagtg cgccatcagc tgaagcacca aaagaaaaag tggaaacatc aggttcagca
    57541 agtgtcgttg aagctgccga tccaaataaa cgtgtattgg ctatgccatc tgttcgtcaa
    57601 tttgcccgtg aaaaagatgt agatatctca caagtaactg caactggaaa aggtggcaga
    57661 gtaacaaaag aggacatcga aaacttcttg gcaggtggtc caagcagtgc accagcgaaa
    57721 tcagaagcgc cagaagcggc agcaccgaaa gaagcagctc ctgcagcaga aagcaaacca
    57781 gctgcaccag caaaaccatt caagtccaac ttaggagact tggaagaacg tgtagcaatg
    57841 acgccaacac gtaaagctat cgctaaagca atggtcaaca gtaagcatac agctccacac
    57901 gtgacattgc atgatgaagt agaagtgtct aaactatggg ataatcggaa acgatttaaa
    57961 gaagttgctg ctgcaaatgg tacgaaactt actttcttgc catatgtcgt gaaagcttta
    58021 acagcgactg ttaagaaata cccagtattg aatgcatcta tcgacgatgc aaaccaagaa
    58081 atcgtttata aacattacta caatatcggt attgcaactg atacggatca cggactatat
    58141 gtaccaaatg tgaaagatgc agaccgcaaa ggaatgtttg ctattgcaga cgaaatcaat
    58201 gagaaagcaa aattagctca tgacggcaaa ctatctgcag aagatatgcg caatggaaca
    58261 attacgatca gcaatatcgg atctgtaggt ggcggttggt ttacaccagt catcaactat
    58321 ccagaagtag ctattttagg tgtaggaacg attgcacaac aaccaatcgt aaatgctgaa
    58381 ggcgaaatcg ttgtcggtcg tgtgatgaaa ctgtcattaa gtttcgatca ccgtatcgta
    58441 gatggagcaa ctgcgcaaca agcaatgaat aatatcaaac gtcttttagc tgatccagag
    58501 ctattaatga tggaaggatg ataaacaaat ggtagttgga gattttgctg tagaattaga
    58561 tacagttgta attggtgcgg gacctggtgg ctatgtagct gccatccgcg ctgctgaaat
    58621 gggacaaaaa gtagcaatca ttgaaagaga gtatattggc ggtgtttgtt tgaatgtcgg
    58681 ctgtattcct tcaaaagcac tgatagctgc aggacaccat tatcaagagt cactagattc
    58741 tgaaatgttt ggagtaacta gtgaaaacgt caaactagat tttgcaaaaa cacaagaatg
    58801 gaaagaaaac aaagttgtcc atacgttgac ttctggtgtt ggtttcctat taaaaaaaca
    58861 taaggttgaa acaatcgaag gagaagcgtt cttcgtagat gatcacacac tacgcgtcat
    58921 tcatccagat tcagctcaaa cttattcgtt caataacgca atcatcgcaa caggttcccg
    58981 tccaatcgag attccaggat ttaaattcgg aggacgtgtg ttagattcta ctggcggatt
    59041 agcattgaaa gaagtaccta agaaatttgt gatcatcggt ggcggtgtga ttggtgccga
    59101 attgggcgga gcgtacgcaa acttaggagc agaagtaacg attcttgaag gctcaccaca
    59161 aattttacca acttacgaaa aagatctagt aaaattagta gaagatgatt tcaagaaaaa
    59221 aggcgtaaca gtcgtaacga atgcaatggc gaaagaagca gtcgataacg gtgacagcgt
    59281 gacagtgaaa tatgcagtgg acgggaaaga agaatccgtt actgctgatt atgtgatggt
    59341 cactgtaggt cgtcgtccga atacagatga tatgggctta gaacaagcgg gtgtcgaagt
    59401 aggagaacga ggattgatca cggtggataa acaaggacgt acgaacgtac ctaacatcta
    59461 tgccatcggt gatatcgttc caggggcggc attagcacac aaagccagtt atgaagcaaa
    59521 aatcgctgct gaagcaatct ctggcaaaaa agtagctgta gattataaag caatgccggc
    59581 agtagccttc actgatcctg aattggcttc tgtaggtatg acgatcaaag aagcaaaaga
    59641 tgctggtatc gaagcaaaag catataaatt cccattttca gggaacggtc gggcattatc
    59701 gcttggtaaa acagaaggat tcatccgtct agttacgacg atcgaagata acgtgcttat
    59761 tggcgcgcaa atcggtggag tcggcgcaag tgatatggtc tctgaattag ctttagcaat
    59821 cgaatcagga atgaatgctg aagatatcgc cttgacgatt catcctcatc catctcttgg
    59881 cgaaatcgtc atggatgctt cggaattagc attaggttta ccaatccata tttgatttga
    59941 ataatacagg cgaaacgcaa ctgaaaa
//
LOCUS       DACDVT010000008        59556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDVT010000008 DACDVT010000000
VERSION     DACDVT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.008"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            159..389
                     /locus_tag="IWL52_000470"
     CDS             159..389
                     /locus_tag="IWL52_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9777058.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            486..1787
                     /gene="murA"
                     /locus_tag="IWL52_000471"
     CDS             486..1787
                     /gene="murA"
                     /locus_tag="IWL52_000471"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9777059.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1800..1976
                     /locus_tag="IWL52_000472"
     CDS             1800..1976
                     /locus_tag="IWL52_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9777060.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2111..2407
                     /gene="yidD"
                     /locus_tag="IWL52_000473"
     CDS             2111..2407
                     /gene="yidD"
                     /locus_tag="IWL52_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9777061.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2592..3059
                     /locus_tag="IWL52_000474"
     CDS             2592..3059
                     /locus_tag="IWL52_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9777062.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="IWL52_000475"
     CDS             3144..4028
                     /locus_tag="IWL52_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9777063.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4145..4378
                     /locus_tag="IWL52_000476"
     CDS             4145..4378
                     /locus_tag="IWL52_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9777064.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="IWL52_000477"
     tRNA            4466..4546
                     /locus_tag="IWL52_000477"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="IWL52_000478"
     tRNA            4553..4624
                     /locus_tag="IWL52_000478"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
     gene            5139..5846
                     /locus_tag="IWL52_000479"
     CDS             5139..5846
                     /locus_tag="IWL52_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9777065.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5905..6549)
                     /locus_tag="IWL52_000480"
     CDS             complement(5905..6549)
                     /locus_tag="IWL52_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9777066.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6675..6962
                     /locus_tag="IWL52_000481"
     CDS             6675..6962
                     /locus_tag="IWL52_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777067.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7070..7390
                     /locus_tag="IWL52_000482"
     CDS             7070..7390
                     /locus_tag="IWL52_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9777068.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7471..8784
                     /locus_tag="IWL52_000483"
     CDS             7471..8784
                     /locus_tag="IWL52_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9777069.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8781..9500
                     /locus_tag="IWL52_000484"
     CDS             8781..9500
                     /locus_tag="IWL52_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9777070.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9628..10179
                     /gene="raiA"
                     /locus_tag="IWL52_000485"
     CDS             9628..10179
                     /gene="raiA"
                     /locus_tag="IWL52_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9777071.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10401..12935
                     /gene="secA"
                     /locus_tag="IWL52_000486"
     CDS             10401..12935
                     /gene="secA"
                     /locus_tag="IWL52_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9777072.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13061..14162
                     /gene="prfB"
                     /locus_tag="IWL52_000487"
     CDS             join(13061..13123,13125..14162)
                     /gene="prfB"
                     /locus_tag="IWL52_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9777073.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14267..14953
                     /gene="ftsE"
                     /locus_tag="IWL52_000488"
     CDS             14267..14953
                     /gene="ftsE"
                     /locus_tag="IWL52_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9777074.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14937..15830
                     /locus_tag="IWL52_000489"
     CDS             14937..15830
                     /locus_tag="IWL52_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777075.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15982..17430
                     /gene="cls"
                     /locus_tag="IWL52_000490"
     CDS             15982..17430
                     /gene="cls"
                     /locus_tag="IWL52_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9777076.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17767..18624
                     /locus_tag="IWL52_000491"
     CDS             17767..18624
                     /locus_tag="IWL52_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9777077.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18739..19659
                     /gene="pstC"
                     /locus_tag="IWL52_000492"
     CDS             18739..19659
                     /gene="pstC"
                     /locus_tag="IWL52_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9777078.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19659..20543
                     /gene="pstA"
                     /locus_tag="IWL52_000493"
     CDS             19659..20543
                     /gene="pstA"
                     /locus_tag="IWL52_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9777079.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20558..21364
                     /gene="pstB"
                     /locus_tag="IWL52_000494"
     CDS             20558..21364
                     /gene="pstB"
                     /locus_tag="IWL52_000494"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9777080.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21380..22138
                     /locus_tag="IWL52_000495"
     CDS             21380..22138
                     /locus_tag="IWL52_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9777081.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22151..22828
                     /gene="phoU"
                     /locus_tag="IWL52_000496"
     CDS             22151..22828
                     /gene="phoU"
                     /locus_tag="IWL52_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9777082.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23065..24096
                     /locus_tag="IWL52_000497"
     CDS             23065..24096
                     /locus_tag="IWL52_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9777083.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24093..24860
                     /locus_tag="IWL52_000498"
     CDS             24093..24860
                     /locus_tag="IWL52_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9777084.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24960..26528
                     /gene="liaX"
                     /locus_tag="IWL52_000499"
     CDS             24960..26528
                     /gene="liaX"
                     /locus_tag="IWL52_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9777085.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26828..27145
                     /locus_tag="IWL52_000500"
     CDS             26828..27145
                     /locus_tag="IWL52_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9777086.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27145..27495
                     /locus_tag="IWL52_000501"
     CDS             27145..27495
                     /locus_tag="IWL52_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9777087.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27662..28600
                     /locus_tag="IWL52_000502"
     CDS             27662..28600
                     /locus_tag="IWL52_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9777088.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28615..29445
                     /locus_tag="IWL52_000503"
     CDS             28615..29445
                     /locus_tag="IWL52_000503"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9777089.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29489..30514
                     /locus_tag="IWL52_000504"
     CDS             29489..30514
                     /locus_tag="IWL52_000504"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9777090.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30529..31467
                     /gene="galU"
                     /locus_tag="IWL52_000505"
     CDS             30529..31467
                     /gene="galU"
                     /locus_tag="IWL52_000505"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9777091.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31592..32032
                     /locus_tag="IWL52_000506"
     CDS             31592..32032
                     /locus_tag="IWL52_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9777092.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32035..32637
                     /locus_tag="IWL52_000507"
     CDS             32035..32637
                     /locus_tag="IWL52_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9777093.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32696..33802)
                     /locus_tag="IWL52_000508"
     CDS             complement(32696..33802)
                     /locus_tag="IWL52_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9777094.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34012..35013
                     /gene="ccpA"
                     /locus_tag="IWL52_000509"
     CDS             34012..35013
                     /gene="ccpA"
                     /locus_tag="IWL52_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9777095.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35350..37695)
                     /locus_tag="IWL52_000510"
     CDS             complement(35350..37695)
                     /locus_tag="IWL52_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9777096.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37974..38876
                     /locus_tag="IWL52_000511"
     CDS             37974..38876
                     /locus_tag="IWL52_000511"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9777097.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39089..40864
                     /locus_tag="IWL52_000512"
     CDS             39089..40864
                     /locus_tag="IWL52_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9777098.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40930..42516)
                     /locus_tag="IWL52_000513"
     CDS             complement(40930..42516)
                     /locus_tag="IWL52_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9777099.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42767..42991
                     /locus_tag="IWL52_000514"
     CDS             42767..42991
                     /locus_tag="IWL52_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9777100.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43133..44884
                     /locus_tag="IWL52_000515"
     CDS             43133..44884
                     /locus_tag="IWL52_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9777101.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44884..46668
                     /locus_tag="IWL52_000516"
     CDS             44884..46668
                     /locus_tag="IWL52_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777102.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46994..47845
                     /locus_tag="IWL52_000517"
     CDS             46994..47845
                     /locus_tag="IWL52_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9777103.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47899..49650
                     /locus_tag="IWL52_000518"
     CDS             47899..49650
                     /locus_tag="IWL52_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9777104.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49654..49824
                     /locus_tag="IWL52_000519"
     CDS             49654..49824
                     /locus_tag="IWL52_000519"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777105.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49864..50766)
                     /locus_tag="IWL52_000520"
     CDS             complement(49864..50766)
                     /locus_tag="IWL52_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9777106.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50997..52400
                     /locus_tag="IWL52_000521"
     CDS             50997..52400
                     /locus_tag="IWL52_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9777107.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52400..53833
                     /gene="ascB"
                     /locus_tag="IWL52_000522"
     CDS             52400..53833
                     /gene="ascB"
                     /locus_tag="IWL52_000522"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9777108.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53978..54904
                     /gene="trxB"
                     /locus_tag="IWL52_000523"
     CDS             53978..54904
                     /gene="trxB"
                     /locus_tag="IWL52_000523"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9777109.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55118..56845
                     /locus_tag="IWL52_000524"
     CDS             55118..56845
                     /locus_tag="IWL52_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9777110.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56917..57210
                     /locus_tag="IWL52_000525"
     CDS             56917..57210
                     /locus_tag="IWL52_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9777111.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57302..58162)
                     /locus_tag="IWL52_000526"
     CDS             complement(57302..58162)
                     /locus_tag="IWL52_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9777112.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58254..58682
                     /locus_tag="IWL52_000527"
     CDS             58254..58682
                     /locus_tag="IWL52_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9777113.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58844..59020
                     /locus_tag="IWL52_000528"
     CDS             58844..59020
                     /locus_tag="IWL52_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9777114.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59047..59493
                     /locus_tag="IWL52_000529"
     CDS             59047..59493
                     /locus_tag="IWL52_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9777115.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19394 a        10331 c        12766 g        17065 t
ORIGIN      
        1 tgtctcagtc cctttttttg catattttta gcatttattt cctaaaaaat gtaaggggat
       61 tcctatattt ttaagtatac ttgtttttcc ttttatacat tttatggtat tatcatttta
      121 ttagttatta taaaacaatc gttagaaagg acgtcattat gcaagtatac ggaattgatg
      181 cggtgcttcg aatcgtgagt catttggcgt ttatctactt agccttctgg tctttacgtt
      241 cgttacgcat tgaaactttt ttcagaccaa tgcataatac gcaaattaga atggcaatcg
      301 tacttttttc aattttttta gggtacactg caagtaattt tttcttagaa gttatcgctt
      361 tatgccgaaa tcttttcgtt agcctgtaaa ttttaaaaaa aggtactatt ttaaaagcaa
      421 ggatgttata atagaaaaga ttttgagtaa gtgtatgctc attggaattt tttggaggga
      481 acaacatgga agagatcatc gtaagaggtg gcaatcaatt aaatggaaca gtaagaattg
      541 aaggagctaa aaatgctgtt ttgcctattt tagctgctag tttgttagca gaagaaggta
      601 ttacgacatt agacaacgtt ccgattcttt ctgatgtctt cactatgaat caagtgattc
      661 gtcacttaaa cgtagatgta gattttgatg aacaaaagaa tcaagtaaca atcgacgctt
      721 caagacaatt agaaatcgaa gcgccttatg agtatgttag tcaaatgcgt gcttcaatcg
      781 ttgtgatggg tccgctactt gccagaaatg gacatgcaaa agtagctatg cctggtggat
      841 gtgcgattgg taaacgacca atcgatttgc atttaaaagg atttcaagct ttaggagcaa
      901 aaattattca aaaaaacggc tatatcgaag caattgcgga tgaattgatc ggtaacacga
      961 tttatttaga tttccctagt gtaggtgcta ctcaaaatat catgatggct gccgtaaaag
     1021 caaaaggaac aaccatcatt gaaaacgtag ctcgcgaacc agaaatcgtt gatttagcaa
     1081 acattttgaa taaaatgggc gctcaagtct atggagctgg tactgaaaca atgcgtattg
     1141 aaggcgtgga tcatttacat gcagtcaacc actcaatcgt acaagatcgt atcgaagcag
     1201 gtacctttat ggttgctgct gcaatgacac aaggcaatgt gctgattgca gatgctatct
     1261 ctgaacataa ccgcccattg atttctaaat tgatcgaaat gggtgcagaa atcattgaag
     1321 aagaaggcgg agtccgtgtc atcggaccaa aacatatctt acctacagat gtaaaaacga
     1381 tgcctcatcc tggtttccct acagatatgc aagctcaaat gacagctatc caattagttg
     1441 cagaaggtac aagtgtagtg actgaaacag tgtttgaaaa tcgtttccaa catttagaag
     1501 agatgcgccg tatgaacgct catgtcaaaa tcgacggaaa tgtagcaatc atggatggaa
     1561 accatgaatt gcaaggtgca gaagtatatg caacagactt gcgggcagca gcagcattag
     1621 ttttagctgg cttgaaagca aatggaatca cacgtgtgcg taacttgaat tatctagacc
     1681 gtggatatta taatttccat atcaaactac agcaattagg tgcagacgtt gaacgtgtag
     1741 acatggatca aacttctgct gaaaaaacag cacaaacgat tgcttaagga agtggctgaa
     1801 tgagtaacca acgatatatc ttattaactt tgataaagat tttaatggtg atcttattgc
     1861 ttatccttct ttttgtggcc ggaacgatga tcggatatgg tgtcatcggt ggcggcaatc
     1921 catttaaagt atttcagcca agtttgtgga ttcatattcg agactttttt cattgataaa
     1981 taagaatcac agtggttcta gtcgatatga agtgagccat gaagcagaac atgataaaag
     2041 aaaacagaag ggaacgttga cgttagtcgc gatccctttt tttagtaaaa gaaaagaagg
     2101 aatctaattg atgaagaaga tattgattta tttagttaga ggatatcagc gctttatttc
     2161 gcctgcattt ccacctagct gccgttatta cccaacctgt tccaattata tgataaaagc
     2221 aattcaaacc catggcgcgc taaaagggac ggtaatgggg actgcacgga tcttacgctg
     2281 tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg acattccgat taacgcgaaa
     2341 taagaaagat aaaaaagaac cagattataa aaaggttgtg aaaaatacgg gtagcactga
     2401 gcgataaaga aacaatttgg aaaatagctt cttatatttt gcaaaattgt gagttattgc
     2461 cgaagtgttg gattttgaac accgtcaaaa aaaaggttgt gaaaaatacg gctagcactg
     2521 agcgataaag aaaaagagga aatttattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta accccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagtg attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaaatttcaa ttcaagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca acttccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgc gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tacggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaatgcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggggtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatattga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat ggaccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4741 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4801 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4861 gggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4921 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4981 aggttggaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     5041 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     5101 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5161 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5221 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5281 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5341 catatgttgc acgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5401 ttatggaatc acaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5461 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5521 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5581 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5641 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5701 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5761 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5821 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattccgt attagaaata
     5881 aaagagaatc agaatcacct gtttttatat atgatcagtt accagttgct cgtgataaga
     5941 aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc tcaatgatgg attctttaaa
     6001 gtggtcagca cttttttcat ctacagcaca gatgacgcta acttgatcag tatattggat
     6061 ttcttttata aaaaaatgtt ggagttcaag ataattctgt aattttccta gttgcggata
     6121 cgtgatcatc acagagatct cctgttgcaa ggtaccttct acgattccaa tgtcagacaa
     6181 tgcttgagag accgcatgag tgtatgctcg tattaatccg cctgtgccga gtttcgttcc
     6241 tccaaaatat cgagtgacta ctgcagctac attgatcagt tgattttttt tgaggacttc
     6301 caacatcggt atgcccgccg ttccacttgg ttctccatca tcgctgcttc gctggatttc
     6361 atttttttct ccaatgatga aggcgctgca gttatggttc gctttccaat gttctttttt
     6421 tatttcttga atgaaacttt ttgcttcgtc ctctgattct attcgtctaa gagaacagat
     6481 aaagcgtgat tttttgatct cgatctcact ttgtccattt tcttttattg tacgatagcg
     6541 ctcgatcatc ttgtcacctc ttttttcatt ttctttattc agtataaata aagttctagg
     6601 ttgtttcaac actgtaacgg ttgcgcaatt gctgtagtat gcgataataa agacaaaggc
     6661 aggtggatct ggggatgcag gaacgaatga aaaagtacga tgcaattacg cattatttga
     6721 agaacaatgg cggctctcaa gtaacgttga cctttaccca atttgacgaa ctgctgtttc
     6781 ccagtaacgg acttcccaaa acagcaaggg aaagtacaga ttggtgggca aatgattata
     6841 aacaccctga aaaaggtgcc tatggctgga taaatgcagg ctatgaagta gttgtcatca
     6901 atttggacaa agaatatgtc gtgttcaata aattagtgaa atctagttgg ctatttgatt
     6961 aaataaaccg cgtttagaaa acgttgacaa atttctttct ttttatataa tagagtaaca
     7021 gacgcgaaga aaacgtttgc gtagtttgtt ttaaaaagga gagaaaagta tggctaaaaa
     7081 aacaatcatg cttgtatgtt cggctggtat gagcacaagt ttactagtaa caaaaatgca
     7141 aaaagctgct gaagcaaaag gaatcgactc tgatattttc gctgtgtccg cttctgacgc
     7201 agataacaat cttgcaaata aagacgtgga tgtgttgtta ttaggcccac aagttcgctt
     7261 tatgaaagct gattttgaga aacgtctgga accaaaaggc attcctttag atgtcatcaa
     7321 tatggctgat tatggtatga tgaatggcga aaaagtgtta gatcaagcaa tcacgctcat
     7381 aggtgaataa atagaacagt ataactatcg aacaagcgat ccaaaaaata gatcgcttgt
     7441 tttttcgtat atttttttag ggcgaataat atgaatcatt tttggggtag aaggcttctg
     7501 acgagagata ttaaagcaat agactgtgag gaggtaaatt taccaaatta tgagaagatc
     7561 gcagcagtgg aacgtttgag caactggatt ctttgtcatc gctgtggaat gagaacgtcc
     7621 gtcattgatg gacagcttgc agattacggt tatttttgta cacattgtct atatttaggg
     7681 cgatgcgaca ctcaacaaga tttatatttg tttgatcagc ctgaagcaaa acctcgagaa
     7741 gtcgtctttt tttggacagg acagcttact gaaaaacaaa cggaaatctc aaagagaata
     7801 ctttgccatt cagaaaaaag acatcatctt atttgggctg taacaggtgc agggaaaaca
     7861 gagatgcttt atcctatttt agtggaaact ttaaaagctg gaggaagagt agcgatttgt
     7921 acaccgcgag tcgatgtttg taatgaattg tttttacgct atcgttatgt ttttccagaa
     7981 gaaaaaatca cgttgctcca tgggaataca gcaacggctt atacctttac ttcttttgtt
     8041 atctgtacga ttcatcagct ataccgtttt tatcggtcct ttgatttaat agtgatcgat
     8101 gagatcgatg cgtttcctta taacggggat gcaggattag ctcatgctgt gcaaacagcg
     8161 attaaaccag acggacgatt catctactta agtgccacac ctgataaaaa gttactaaag
     8221 gagatcaagc agacgtttga catccacaaa cttgctttac gctttcatcg gcgtttgctt
     8281 cctgaacccg tcttggtttt catgaataat tggcgacaaa aatgcaagaa caagaagaag
     8341 ataaaatctt tggtgcggat cattcatcaa ttaattaaag aaaaccatat ccttttattt
     8401 tgtccaagta tcacgatgat gcatcgtttg aaagccacac ttcaagaggc tcttccgaga
     8461 tatgagatca cagatgtttc ttcaagagac aatcagcggg cggaaaaagt ccagaaaatg
     8521 cgagaagcaa aatatgacat actgttaacc acgatgatcc ttgaacgtgg agtcacattt
     8581 gaaagaatat ccgttatcgt tttaggagcg gatcatccgg tttttactaa atcttctttg
     8641 gtacaaatcg caggaagagc cgatcgtaaa gggccttata cgaacagtca agtatatttt
     8701 ttctatgaag aaaaaactgc agctattcga aaagcttgtc aggaaatcaa agaaatgaat
     8761 gaagaaggga agaagatact gtgaggtgca gttgctgcag taaagatatc gtccaaaatt
     8821 taacattcag cgagattatt ggtgtttcga aatacagatt acctcagact tgttcagttt
     8881 gtcgcgcaca atttcattta ttggaaacaa gaggctgttc tggctgtcaa aaaccaatcc
     8941 atttcaatct agagagtacg aaaaagaaag taaattctat ccccgtttac tgcagtgact
     9001 gtcaagaatg gaagaagaaa tatccgacct atgattttca gcaccgtgcc ttttttgtgt
     9061 acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt tatgggagat atccgactag
     9121 caggcacctt tgcatataaa tggacgcaga taaaaaaaga atacccgaca tacatctatt
     9181 gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg attcaatcaa gtaagcgaga
     9241 tgttgaaagc agcaagaatt ccttttcaga tgctactcaa aagagagaag catctgctgg
     9301 ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc tcaacctttt gaacttgcag
     9361 tcaccttttc taagatacaa aataaaaaaa tattgattat tgatgatgtc tatacaacag
     9421 gacagactct tttccacgca gcagattgtt tgcagatcgc acagccgaaa atcatccgaa
     9481 ctatttcttt ggcgagatga attagatgtg gaaatttgat agcgatttaa tttgcaaata
     9541 acctagagtt cgatataata gaattaagaa gagggaaaga gtggtccttc tccttcttga
     9601 ttgtggtaga cgaaaggggt aatttttatg tttagatata atgtacgcgg cgaaaatatc
     9661 gaagtaactg aagcgattcg cgactacgtt gagaaaaaag taggcaaact agaacgctat
     9721 ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa aagtttatac agaaaaaaca
     9781 gcaaaagtgg aagtaacgat cccattacct tatcttgtat tacgtgccga agaaacttct
     9841 ccagatttat acgcaagtat cgatttggtt gttgacaaat tggaacgcca aatccgcaaa
     9901 ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca tgaatactgc aaatgctgct
     9961 gctttattca atgaagagga cgaaaacgaa aatgattctg aattagatat cgtacgtacg
    10021 aaacgccttt cattgaaacc aatggatagt gaagaagcag tcttgcaaat gaacatgctg
    10081 ggacataact tctttatttt tgaagatgca gaaacaaatg gtacaagtat cgtttaccgc
    10141 cgtaaagatg gaaaatacgg actgattgaa acagactgat cagccttact taaataacaa
    10201 cgaaaacttg cagcagctac tgacagctgc cgcaagtttt tttgagtttc ctcctctttt
    10261 agatgaaata tgaataaatt atgaagttct tactttaata cgcttaagat atttcaatta
    10321 tctataacta atgataaaat gaaaagtaaa gtctacatag tctggataat acacagaagg
    10381 aaaaggagat tacacaacgc atggccaatt tgattcgttc acttatcgaa aacgataaga
    10441 aagaactaaa acgtttagat agcattgcaa aaaaagttga agcatacgct gaccaaatgg
    10501 cagcattaac tgatgaacaa ttacaggcaa aaactgctga attcaagggc agataccaaa
    10561 aaggagaaac attagatcaa ttacttccgg aagcttttgc agtcgtccga gaagcagcaa
    10621 aacgtgtatt aggtttatat ccataccacg tacaattgat gggtggaatc gtgcttcacg
    10681 atggaaatat ccctgaaatg cgtactggtg aaggtaaaac cttgaccgca acgatgccag
    10741 tatatctaaa tgctctgact ggcgaagggg tacacgtagt taccgtcaat gaatatttag
    10801 caacccgtga ctcgacggaa atgggcgaac tttataattt cttaggatta acagtggggt
    10861 tgaatatcaa ctcaaaatct tcggatgaaa aacgcgaggc ttataattgt gatatcactt
    10921 atagtacgaa taatgaatta ggatttgact acttgagaga caatatggtc gtttaccgcc
    10981 atcaaatggt acaacgtccg ctgaattatg ccattgtcga tgaagttgac tcaatcttga
    11041 ttgatgaagc gagaacgccg ctgatcattt cagggcaagc agaaaaatcc acagctttat
    11101 atacaagagc agacaacttc gtgaaacgtt tgaaagaaga agaagattat aaaatcgatg
    11161 tacaatcaaa aacaatcagt ttaaccgaag cagggatcga aaaagcagaa gaaacattcg
    11221 gtttggataa cttatacgac attgaaaata ctgcattgac ccatcacttg gatcaagcat
    11281 tgagagccaa ctatatcatg cttttagata tcgattatgt tgtccaagac ggaaaagtac
    11341 tgattgttga ccagttcact ggtcggatca tggatggacg ccgttattca gatggacttc
    11401 accaagcaat cgaagctaaa gaaggcgtag agatcgaaga cgaaacgaaa accatggcga
    11461 cgatcacttt ccagaactat ttccgtatgt acaagaaact tgcgggaatg actggtacag
    11521 ctaaaacaga agaagaagaa ttcagagaaa tttataacat ccaagtatac cagattccga
    11581 caaatcgtcc ggttatccgt gatgatcgtc cagatttgct ttacccaaca ttacaaagca
    11641 agttccacgc tgtcgtgcaa gatatcaaag atcgctaccg caaaggtcaa cctgtgttag
    11701 tcggaactgt tgcggttgaa acatctgagt tattatcaga tatgctgaat caagaacgta
    11761 ttcctcatga agtgctgaat gcgaaaaatc actttaaaga agcagagatc atcatgaatg
    11821 ctggtcaaaa aggtgctgtg acgattgcta ccaacatggc tggtcgggga acagatatca
    11881 agttaggact tggggtaaga gaacttggtg gtctagcagt tatcgggaca gaacgacatg
    11941 aatctcgtcg tatcgataac cagcttcgag gacgtgcagg acgtcaagga gaccctggta
    12001 tgtcacagtt ctacttgtca ttggaagatg atttgatgaa acgattcggt tcagaacgaa
    12061 tcaaagcttt cttagatcgg atgaagatcg gtgaggaaga tgctgttatc caaagcaaga
    12121 tgctgacgaa acaagtagaa tctgcacaaa aacgtgtaga aggaaataac tatgacacac
    12181 gtaaaaacgt cttacaatac gatgatgtga tgcgtgaaca acgtgaagta atctataaac
    12241 agcgacaaga agtaatcatg gaagaaaaaa gcttatccaa gccactaatg aacatggtca
    12301 aacggacaat cagtcgtgta gtagatgcgc atacacaatt agagaaagaa aattggaatt
    12361 tggaaagtat catcgatttt gccggcagta ctcttgtcca tgaagatagt attactcttg
    12421 cagatatcga aggaaaaaca gctcaggaga tcaaagagta tctaaacgaa cgagcacaag
    12481 aagtatttaa taccaaagcg gctcaactaa atggtccaga gcaactgttg gaatttgaaa
    12541 aagtcgtgat cttgcgtgtt gtggactcta aatggacaga ccatattgac gcaatggatc
    12601 aattacgtca gtcaatcggc ttacgtgctt atggacagaa caatccatta gtcgaatatc
    12661 aaacagaagg ctataagatg tttgaagata tggtaggcgc catcgagtat gaagtcactc
    12721 gaatctttat gaaagctgag atccgtcaaa acgtacagcg tgaacaggtt tctcaaggaa
    12781 cagcttctca ctcagaagac ggagatgcca aagaaaatag taatactagc gcgaaaaaac
    12841 agccaatcca tgtgaacaag gtcggaagaa atgatccttg tccttgtgga agcgggaaaa
    12901 aatataagaa ctgtcatgga aaaaatgcaa actaatttct gacagcagga ttggtgagac
    12961 aaattctttt tttaagaatt cgtctcacct tctttctgtt atttgctgat tctgttagac
    13021 tagatataat gaaaaaagta tagtgaaaga aggaaaaaac atggaaatat ccgaaattcg
    13081 aaatctcctg acagaaatgc aggaaaaggt cacaagtttc aggaggtctc tttgacttag
    13141 atcagctgga agaagacatc gcggaagcag aagcaaaaat ggctgaacct ggtttttggg
    13201 atgacagcga agccgcacaa aaagtgatca gtgaaaataa cgctaataaa gaaacctatg
    13261 accaattcca ttctcttgcc gaagaactag aagaaatcga attgatggtg gagatgctgc
    13321 aagaagaacc tgatgcagca atgcaagaag aagcagaaac acgtatcaat gcattagatg
    13381 aaaaaatgaa gacgtatgaa ttatctatgc tgttagacgg accttatgat cgaaacaatg
    13441 cgatcgtcga attgcatcca ggggctggcg gaacggagtc ccaagactgg ggcagcatgc
    13501 ttttacgtat gtatacacgt tgggcagagc agcatggcta tcaagtagaa acactagatt
    13561 atcaagcagg tgatgaagca gggatcaaga gtgtgaccct gctgattaaa ggacataacg
    13621 cttttggtta tctaaaatca gaaaaaggtg ttcatcgcct tgtacggatt tcgccttttg
    13681 actcagcgaa acgacgtcat acttcttttt gttcggtaga tgtgatgcca gagcttgatg
    13741 aaacgatcga tatagaaatc aatccagatg atttgaaaat cgatacgtac cgagcaagtg
    13801 gtgcaggtgg tcagcacatc aataaaacag aatctgctgt acggatcact cacttgccta
    13861 caggaacagt agtagctagt caggcacaac gctcacaatt gaaaaaccgt gagcaagcga
    13921 tggggatgtt aaaagctaag ctttatcaat tagaagtgga aaagaaagaa caagaagcag
    13981 ctgcacttcg aggagaacaa aaagaaatcg gctggggttc acagatccgt tcttatgttt
    14041 tccatcccta ttctatggta aaagaccatc ggacaaacgt agagacagga aatgtccaag
    14101 cagttatgga cggtgacctt gatttattta ttgacggcta tttaaaaatg catctacaat
    14161 aagaacccat tgttaaattg aaataaaatt gtaagatttt gcaagcatgt tgaaacattg
    14221 ccatgctata atagctaaag actgaaaaag atatggagag aacgccatga ttgaaatgaa
    14281 ggatgtaatg aagaagtact caaatggtac gaccgctatc cgcaatctct cgattgtcat
    14341 cgaacaagga gagtttgtct atgtggttgg tccgtctgga gccggtaaat ctacattcat
    14401 caaattgatg tatcgagaag aaaaagcttc aaaaggaact ttgaacgtcg caggtcatga
    14461 tctaatagca atcaaaaacc gcgaagttcc ttatctacgc agagaaatcg gcgtggtgtt
    14521 ccaagactat aaattattac caagaaaaac agtatatgaa aatgttgcgt acgctatgca
    14581 agttatcggc agaaaacctc gagatatcaa aaaacgagta atggaagtgc tggaccttgt
    14641 tggtttgaaa cacaaagttc gagtctttcc tagcgaatta tccggtgggg aacagcaacg
    14701 tgtctctatt gctcgggcaa tcgtgaatac gcctaaagta cttatcgcgg atgaaccgac
    14761 aggaaatctg gatccagaaa actcatggga aatcatgaaa ttattagatc ggatcaatgc
    14821 ccaaggaact actgtcgtga tggctacaca taacagtacg atcgtaaata cgattcggca
    14881 ccgagtaatt gctatcgaaa acggccgcat tattcgagat caagcggaag gggaatatgg
    14941 atacgatgat tagaaccttt ttccgccatt tattagaaag tatcaagagt ttgaaacgta
    15001 acggctggat gacagttgct tcagtcagtg cggtaacgat cacgttgact ttagtcggtg
    15061 tattcatggc agttatcatg aatgcaacta aattagctca ggatatagaa ggaaacgttg
    15121 atgtatctgt attcgtagat atcggtacaa aagacgaaga gatgaaaaag ctagaaaaag
    15181 aattgaatga cttagatcat gttaaaaaag ttgaattttc aagtaaagaa caggaattga
    15241 aaaaaatcca aaacgaaatg ggtgattcct ggaaattgtt tgaaggtgac aataacccgt
    15301 tatatgatgt gtatgtagta agtgctactg atccttctta tacaaaacaa gtagcagaag
    15361 aagcagcaga tttgaagaat gtttctcgtt ctgattacgg tggagcttcc tcagatcgga
    15421 ttttccaaat cgctggtgca gttcggacat ggggattagt tgcagcggca ttgctgctgt
    15481 tcgtagcgat gtttctcatt tcgaacacga ttcggatcac gattttgtct agacagcgtg
    15541 aaattcaaat catgcgttta gtcggtgcaa aaaatggata tattcgctgg ccgtttttcc
    15601 ttgaaggtgg ctggatcggt ctgatcggtg cgattcttcc gattttgatc atcacatttg
    15661 gttatgcatg gtttttccaa ttgatcaatc catcattatt acgttcccat tattcattga
    15721 ttcatccaca aaatatcgtt tggaaaatca atcttttaat ggtcggcatt ggtgtgatca
    15781 ttggttcact tggctcgatc atttctatga gacgtttctt gaaattctaa taccacaata
    15841 taaataaaat gagaaagttg gacagtttcg ttccagcttt ctttttttat tggctaggag
    15901 aatagaagga agcggctttt ttccctttac tttaaaaaac aacttgggta ttcttaagtt
    15961 ggtagaggaa gaggtgaagc cgtgaaaatt atcgttgaca atttttttac gatccttttg
    16021 attatgaata ttttgctttc ctttatcatc gtttttcgag aaagaaagga aactgctcaa
    16081 acatgggcat ggctgctcgt tttgatgttc attccagttg taggatttat tttatatatt
    16141 tttttaggaa gagggatttc caaagagaaa atctttgatt tgaagatcca agggaaaatc
    16201 ggtaagaacc tggaaattga agaagacaaa caggcattga tgcgagggct ttatcctcat
    16261 ccgcctactg gacaagtaga tgtgaaacaa ttgatctata tgctcacggt atttgaaagt
    16321 acgctctata cgactaaaaa tgaaatcact ttatttacag atggtcgtga aaaattcgat
    16381 gcgctgattg aagatatcaa gcaagcagaa gaccatatcc acttccagta ttatatttat
    16441 cgcagtgatg cattaggtga agaagttcga gatgcattga cagaggcggc aagacgaggg
    16501 gtaaaagtca gagtccttct tgatgcatgg ggttccacac aagtttcttc cagctttttt
    16561 cagaatttga aaaaagcagg cggagaaatc gcatttttct ttcctctatt tgttccgtat
    16621 attaatcctc ggatcaatta tcgcaatcat cgtaaaatcg tagttattga tgggaaaatc
    16681 ggatatacag gtgggttcaa tgttggaaat gaatatctag gattggtaaa gaaatttggt
    16741 tattggcgag acaatcattt gcgtatttac ggagaagcgg tgtatatctt gcaaaaccgt
    16801 tttttgatgg actggaattc tcagcataca aaagaagtag gctattctcc caaatttttt
    16861 ccgtccattc actctactgg tgatatagct gttcaaatcg tgacaagcgg accagatacc
    16921 gaacatgaac aaatcaaaat gacttatttg aaaatgattt cgttagccaa acgggagata
    16981 ttgatccaaa cgccttatta cataccggat ggatcgattc atgaagcact gaagttggct
    17041 cttctttcag gcgtaaaagt tcatatccaa atacctaaca aaccagatca tcttctcgtt
    17101 tactgggcaa cgtattcttt cgctgcagag ctgatcgagt atggagcaag gatcgaaaca
    17161 tatgagaatg gatttattca tgcaaagacg atgattatcg atggtggcat tgtttcagtt
    17221 ggctcagcaa atatcgatgt tcgttcattt cgcttggact ttgaggtcaa tacactgatt
    17281 tatgatgaac gaatggcttc acgtgtaaga aaagcatttt ttgaagactc gaaaatttct
    17341 acgcatctca caaaagaaat gtatgaaaac cgaggaataa tcatcaagat gaaagaagga
    17401 ttagcaagat tgatttcgcc gctcctttaa aacacaaaaa ggacatgaga accatcaaca
    17461 gatggagatc tcgtgtcctt ttctaacggg gccatttctg agagtacgtc agaaatggag
    17521 aaagcgtctc gcatgagcga aattaacttt ggggtatctc ttgttttttg gggaaagcaa
    17581 gaaataagct aatatatgct attaaaagag acacatttca aagccagtat agcatagaaa
    17641 cttttaatga gatagttttt tttacaatta tgaggattta ttttgttatt taatgatttt
    17701 gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga ttaaaggaga
    17761 tttctaatga aaaaacttaa attattctta ccaataatag gattagctat tcttttatca
    17821 ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc ttctgcttta
    17881 cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg aaaattcatc
    17941 aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg agcagtcgac
    18001 atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc agcattagtc
    18061 gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat tggcgtaaaa
    18121 gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa ctggagtgaa
    18181 gtcggtggaa aagatcagcc agtagttata ttgaaccgtg cttcaggaag cggaacccgt
    18241 agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca agaacaagat
    18301 tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag ctatgttgct
    18361 ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga accgacagat
    18421 gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta cacaagagat
    18481 catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga agtacaagat
    18541 aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga acgtgattgg
    18601 caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag ctaaactttt
    18661 acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat gttaaacctg
    18721 attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa atcaaaacgt
    18781 tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct gatcgtgttg
    18841 gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt ttttactgat
    18901 aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca aatcggagca
    18961 aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat tgtgaccttt
    19021 ttatctgcta ttgttgccac accatttgcc attggtgcag cagtattcat gacggagatc
    19081 tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt aggtattcct
    19141 tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag aagtattttt
    19201 ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat gatcttgccg
    19261 actgtcacaa cgatgactgt tgatgcatta aaggcagtac cacggcacta tcgggaagct
    19321 tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct acgagcagcc
    19381 gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg tgaagcctta
    19441 gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac tactcctgct
    19501 tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg aacagttgaa
    19561 aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt ctttaatatc
    19621 gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa cgctcagata
    19681 aaattgctac aggtgtttta tatgtagttt cggggatcat cgtattgatt ttagcagctt
    19741 tactacttta tatcttagtt cggggaatac cgcacatttc atggtccttc ttgaccgaac
    19801 cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac tcgatttatt
    19861 tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt atctatcttt
    19921 cagaatatgc tggaaaaaac tggctgacag atgtgattcg cacgtcgatt gaaatcttga
    19981 gctcattgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt attcaattcc
    20041 agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac ttgcctcttt
    20101 tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga gaagcaggat
    20161 tggctttagg tttgtcacgc tgggaaacag tgataaaagt ggtggtttca gaagcgacac
    20221 cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa gcagctgcat
    20281 tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg aatccattaa
    20341 gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc catatttgga
    20401 agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc gcttctgccg
    20461 tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc aaacgattat
    20521 accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata actggaatga
    20581 aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg atgatctaca
    20641 tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg aaaaaaataa
    20701 gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc gttcgttgaa
    20761 tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt atcaaggcgt
    20821 agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg gcatggtttt
    20881 tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg ctttgaaaca
    20941 gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct tgaaacaagc
    21001 tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt tatctggcgg
    21061 gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata tcttgctgct
    21121 agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag aaacacttgt
    21181 aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac aagcggctcg
    21241 tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg acaaaactaa
    21301 caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg gtcattttgg
    21361 ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc ttcatctgta
    21421 ttatggaaag aaagaagcac taaaagggat cgatctaagt ttcaatcaag gggaaatcac
    21481 tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt tgaaccggat
    21541 gaatgacctg attccgaatg taacgatcac cgggagtgtt ttatataaag acaaagacat
    21601 ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg tattccagca
    21661 gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga aattaaaagg
    21721 ggaaaaaaat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg cagcgtctgt
    21781 ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg gtgggcagca
    21841 gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct tattggatga
    21901 accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc tgttagaatt
    21961 aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt cacgtatttc
    22021 agataagacg gcattctttt tagatggtca cttgatcgaa tacaatgata cgaagaagat
    22081 attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat ttggataagg
    22141 aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc ataatcagtt
    22201 ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg catacatcaa
    22261 acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca ataacatgga
    22321 aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag ttacaactga
    22381 tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta tggccgatca
    22441 tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa taccggaaat
    22501 cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca acgtcctagt
    22561 tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg atcaaagagt
    22621 caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg aaaatcctga
    22681 gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac ggatcggaga
    22741 ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa tcactgagtt
    22801 aaatacgaac cacaatgaga ttttctaaac taaagcggtg attctgccgt ttgtttagga
    22861 aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    22921 tcaaacaaaa atagctcctc atatttttca tttggctgga cttattaccg cagatgcaag
    22981 cttttgaaca ctgtttattc gggaaagggt ttgtgacaaa atatgtcaca aacccttttc
    23041 atcatttcat agaaaggata aagaatgcaa aaaaaaatta gcatcatcac tatatttttt
    23101 atttgtgtgc ttacttcatg ggttattcag catcaagctg ctttatatga agtacctgtc
    23161 atcgagatcg aaaaagtacg aataaaagag gatcatcaat tgattacagg aagactgatg
    23221 aacacgaatc aaaaaggaga atcgatttat ttatcggttc cttatcaaaa aaatcaaata
    23281 gaaacagcaa atataaaagc gagacaaatg tatctagtcc aaatgcatgg agatgaatgg
    23341 cagctagtag caaaaaaaaa tgatggctgg cttttcttct tcacaagtat cttcattggc
    23401 gccatgttgc ttattggcgg aaaacatggt ctatttgctt taggaagtct catcatcaat
    23461 atcgttttac ttttactttt gttgctcatc tttactaaaa tagaagggat ttctctatta
    23521 aatatatcta tcctgttcgt cgttataggg atgattatta gcatattggc tttagatgga
    23581 tggaatagaa gcagtataat caaaatttca gctgcagtta cgagtgtttt tttagcattt
    23641 tttatttgtc ttttgacaat gaatctttgg aaagaccaag gactgcgcta tgaggaactg
    23701 aattatctga cacgaccgta tcgttctgtt tttctttcta gcgtactgat tggaacagct
    23761 ggaggaacac tcgataccgt tatcacagtc attgcgacat tagaagaatt gactaagcga
    23821 caaccagaaa taacagcgaa acagttatgg ctttcgggaa ggcaagtggg tcgagatgtg
    23881 attggctcga tgaccaatat ccttttgttc gtctatatca gtgggtcagt tccatcgctt
    23941 cttttatatt taggaaatca atggtcgttc aaagaaacga tagaacagca tttatcattg
    24001 gaatttttac gcgtcattgc tggcagctta ggaatagtat tatcgatccc tatctcggtc
    24061 ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt acttattata cttgttggga
    24121 ttttagcaat cagcgtgcta cttattggcg gtacgcaagg tatgtatatt cttttaggct
    24181 tattcatcaa tttagggatc tttttcttgt tgttattcgg ttatcatcag aagtggccga
    24241 tactggcatt gtcggtcata ggatttttat tgattgctgt ggttatctta ttttttatca
    24301 acggttataa tctaaaaatg cgtgcagctt ttgtgagtat actgatcttt ttattttgtt
    24361 tcctactatt gcttcctatc actgattttt tagcaatcca aggttttaca acgatagaac
    24421 tagaagaatt atctggctta gataagactt tagcgattga ttttagatta cttacgagat
    24481 cgttgcttct gattagtctt agtggtgctg ttttagatgc gagtgtagca atttctagtg
    24541 ggacattcga agtatatcag gcaaatccgc aattatcttt ttaccaattg cgccattcta
    24601 gtttcgctat tgctaaaaaa atattggcgt caacagtaat gacacttctt tttgcattca
    24661 tgggaagttc tttagcgttg atcctctggt ttattgattt agacattcct tttcagcaag
    24721 tgatcaatga aaaatcattt gttctagaat atacgatggc tattcttacc acattgtccg
    24781 cactacttgt tcttccttta acctgcgtga ctacagcata tctctttaca aaaaagaaag
    24841 aacaactcaa gatggagtaa aagttacaac ttaagtccta tgacattttt actgaatcag
    24901 tgcataatat ggatgtaagg aaaagcttgc aaacaatttt gcttaaggag gcaatttcca
    24961 tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg aatcctttcg actgaagaag
    25021 gcttagattt attagaaagc atggcaactg aaaaagacga aaagcaaatc aaaaaagaag
    25081 cagacaaggt aactgctagt cataaagaaa aagatcaggc aagtcaattg atcgataaac
    25141 ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc aaaggaaaga gaaagacaag
    25201 atcaagaaaa cttagaaaag atcttagatg aactagcaac tgaagcgaat aaaacttcgg
    25261 cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa ggcagcacgc aatgagaaac
    25321 aggaagcatt gatgcagctg aatacaaaag aagaattagg cgagttgaca gaagaagaat
    25381 tagcacagcg tcagacgtta gaagcagaaa tcaaagagtt agaagctagt ggcgatacac
    25441 tgttagaaga acaattgaaa ttagaagcag aattaaaaga tatccgtaag aatcaatggt
    25501 ctgagaaaaa agaaacgttt actgataagt ttgaattgcc agatgattgg aaagatcaag
    25561 caacagatac actgaatcaa gtaggcgaaa aaatgtcaga agctggatca cagttaggaa
    25621 aattcttgaa gaaaacattc caaactgttt cagaaacagt gaatgacaac atggaatgga
    25681 aagatgtcag cttgcgtgtt cctgggattg cgacaacgaa atttgagcat gaattttact
    25741 atgaagctcc tgcagcaagt atccttgata tcaaggcagc aaacggaaac gtgacgttaa
    25801 aaacgtggga cagcaacgat gtgaaagttg aagctaagat caaactttac ggaaaaatgg
    25861 gtgctgaacc atttgaagca ttctctgaac ggagccagat tgaggttaat gaagatcata
    25921 tctccttcca aattccaaat aaacgtgtgc gtgcagacct tgttttctac ttacctaaac
    25981 gagtatacga ccacgcagct attaaacttc taaatggcaa catcatgatt gaaacattgg
    26041 aagctaaaga tatttataca aaatctacga atggtaacat catcgtggac caattaactg
    26101 caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga tattcgaaac gggaacatct
    26161 tagattctat tatcgaaaca gtaaatggta cagtgacatt tggtgcgaca gcagaaaacc
    26221 tttctgtgtc tttggtaaat ggtgatgtac gtcttacgat taaagaagat aatttgaaaa
    26281 aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc tttaccagat ggcatcggtt
    26341 tggaaggaca tgctaaaaca agtcttggaa gtatcaacag ccgtctgtcc aattacgaag
    26401 tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca attccgtcgt gtttcagatg
    26461 gtgaaatcgc gcaagtgcaa ctttcaacga caacaggaag tatctattta aaagatacag
    26521 ataaataaag aggttgtgaa agagatgttc agccttgagc aataagaagc gattttgtaa
    26581 aatagcttcc cctattttac aaaattgtgc cttattgccg ataggctatc tcttgaacac
    26641 cgtttaccaa gagattatga acttgccaat tatcgccgag tcacaaggaa taatttcaaa
    26701 aaaagctcgt cacgttttat gaatatggta atttgtgacc gagaattttg caaaaagaca
    26761 ccgattaaga agaaggttgt cacaacgatt ttcttcattt aaaatagtag aacagaggtg
    26821 agacatgatg aaaaaaaagt tgacaaaatc caataaaaat gttgttttga cagggacttt
    26881 agcaggaata ggagaataca tcgggattga tccgactgtt atccgtgtgc tttatgtatt
    26941 cctgagctta gttgcttttg gaagtccaat cattttatac attttattag ctttgattat
    27001 cccatctgcg cgttctgcac gaacaaacta tggacataac aatgcatatt atgaaaaaaa
    27061 caattatcgt gaacaaactc gtgaacaaac aaaaaaacgc aaagaagcgg aaaaaatcga
    27121 tgatgacgaa tggagtgact tttaatgtcg tactttcaac gtattttagt taatacgtta
    27181 acattcatat cattggctgt tctttttcct gcgatggtcc atgtgagcag tatttggacg
    27241 gccgttcttg ccagcttcgt attgtctgtg ttgaatatgt ttgtcaaacc gattttgacg
    27301 atactgtccc tgccctttac attagtcaca ttgggattgt ttacgtttgt tatcaatgca
    27361 gcgattctta aaatgacctc cttttttgtg ggagcagaac attttagttt ttcttcattc
    27421 ggtgcagcgt tgttagtagc tatcttgatg tcgcttgtca atgtaattgt cagcgaacat
    27481 aacagtagaa aataaaagaa agctgtgccg aactatcggc gcagtttttt tatttagaat
    27541 caggaatgcg ttttctatta cattggtaat taaataaaaa cgtttttttt aattggtagt
    27601 acttttggct agacaatgat aaaatgatag agaacactct aatagaaatg aggtagcatg
    27661 aatgtcagaa gttgtaaaag ttagccaatt agtgaaaaaa ttatctttgg aaatcgtgac
    27721 aggcgacgag aaaagcttag accgtgtcat cgtaactggg gacatctcac gcccaggact
    27781 agagttgact ggttatttta attattattc ccataatcgt ttacagctat ttggcagtaa
    27841 agaaatcagt tttgcggaac ggatgatccc agaagagcgt ttgatcatca tgagaagatt
    27901 atgtagtgaa gatatgcccg catttattat ttcaagagga ttaccagctc cagaagaaat
    27961 gatccaggca gcaaatgaaa atgggatagc tgtactgcgt tctcccatct caacttctcg
    28021 cttattagga gaattatcca gttatttaga tggtcgcctt gcccctcgaa caagtgtcca
    28081 cggtgtttta gtggatgttt acggattagg tgtactgatt caaggtgaca gtgggatagg
    28141 aaaaagtgaa acagcgttgg agttgatcaa acgcggtcat cgattgatcg cagacgatcg
    28201 agtagatgtt tatcagcaag atgaattaac ggtgatcgga gaaccgccta aaatcctgga
    28261 acatctgatt gaaatccgag gaatcgggat cattgatgta atgaatcttt ttggtgcgag
    28321 tgcggtacgt ggctctatgc aagtccagtt ggctgtttat ctggaagcat gggcaaaaga
    28381 taagaaatac gaccgtctgg gctctgaaga cacgaccgtt gaaatcgcgg aagtaggcat
    28441 tccacaaata aaaatccctg tcaaaacagg acgaaacgtt gcaatcatca ttgaagtagc
    28501 cgcgatgaat ttccgtgcac gtacaatggg ctttgacgca acgaaaacat ttgaagaaag
    28561 attaagtcgc ttgattgaag aaaactcagg caacgactag caaggagtga caatatgtta
    28621 gcacaaatca atcgagttgc ttttgagctt gtagggattc ctatctactg gtatgcattg
    28681 atcatcgtat caggtatcat tatcgccatg tggcttagca gcagagaggc tgttcgggta
    28741 ggattaaaag cagatgatgt gacagatttt atgttgtggg gattgcctat ctccattatt
    28801 ggcgctcgct tatattatgt tttatttgat cttccccagt atatagctga tccaatccaa
    28861 atattcaata tccgttcagg aggcttagct atttatggcg gactgattgc aggcgcgatc
    28921 acgctgtatt tttttactaa atatcatttt atttctttgt ggactttttt agatatcgct
    28981 gcaccaagtg tattattagc acaagcaatc ggtcgttggg gaaatttcat gaaccacgaa
    29041 gcatatggac cagacacgac gcgaagcttc ttagaaagtc tgcatttacc gcaattcatc
    29101 attgataata tgtatatcga agggatctat cgtcaaccaa catttcttta tgaatcactt
    29161 tggagtttga ttggatttat tttgcttttg tttttacgaa agaaaccaca ccttttgaaa
    29221 aaaggtgaag tccttttatg ttatctaatg tggtatagtt tcgggcgttt ctttattgaa
    29281 ggattacgta cagacagtct ttacttttta ggtgtgatcc gtatttctca attattatct
    29341 gctgtgttat ttgtcggtac attgattcta tttatttggc gcagaaaaaa aggaaattta
    29401 ccggattatg accgctcatt aggaaaaaat caacaaatta tctgattttt agagcgttaa
    29461 aaatgaaaaa aatttcagga gtggtaaaat gaattataga gtagcggttt taggaccagg
    29521 ctcttggggt actgctttgg cacaagtcct cgccgaaaac ggacatgacg ttcggatttg
    29581 gggacatcgt gcttcacaag tagaagaaat caacacccaa cacacgaatc aacgttattt
    29641 accagatcat tgtctacccg attccatcca agcattcacc aatatggaag atgcagtaaa
    29701 agacgcagat gcggtgctat tcgtcattcc aacgaaagca atacgttctg ttgcaaaaga
    29761 actagtgcct aaattgaata caaagccagt gatcatccat gcaagtaaag gattagaaca
    29821 ggggacacat aaaaggatct ctgatatctt gttagaggaa atcccagtag aaaaaagaaa
    29881 agctgttgtt gttctttctg gaccaagcca tgcagaagaa gtagcgaaac atgacatcac
    29941 tacgatcaca gctgcaagta cagatgcaaa agcagcaaaa tatgtacaag agctgttcat
    30001 gaataattat tttcggatct ataccaatcc tgatgtgatc ggtgtagaga tgggggcagc
    30061 tttgaaaaat attattgcta ttggtgctgg cgctattcac ggcttagggt ttggcgataa
    30121 tgcaaaggca gcaatcatga cgcgcggatt agctgagatc agccgtttag gtgtagcaat
    30181 gggtgccaac ccgctgacct ttatcggatt aagcggagta ggcgatctag tcgttacttg
    30241 cacaagtgtt cattctagga actggcgcgc aggcaacttg ttaggacaag ggcagcccct
    30301 agaagaggtg ctagccaata tgggaatggt cgttgaaggt gtgaatacta cccaagcagc
    30361 gatggaacta gcccaaataa tggaaatcga aatgcccatc actcaaacaa tctatgaagt
    30421 gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa atcatgttac gtgatgggaa
    30481 aaaagaaaat gaattctcat taacaatttt ttgaaggagc gaatgtatat gaaagtcaaa
    30541 aaagcagtta ttccagcagc aggattagga acaagatttt taccagcaac aaaagctatg
    30601 gcaaaggaaa tgttgccaat tgttgataaa ccaacaatcc agttcatcgt tgaagaagcg
    30661 ttgaaatcag gtatcgaaga catcttgatc gttacaggaa aagctaaacg accaatcgaa
    30721 gatcactttg attcaaatat tgaattagaa acaaacctaa gtgaaaaagg aaagaccgaa
    30781 ttattgaaat tagtagaaga aacgacagat gtcaacttgc atttcatccg tcaatctcat
    30841 ccaaagggtt taggacatgc cgttcttcaa gcaagagcct ttattggaaa tgaaccattt
    30901 gtcgttatgc taggcgatga tataatggaa gacgaagtac cattgacaca gcaattaatc
    30961 gatgattacg aacgtactca tgcttctacg atcgccgtta tgcaagttcc tcatgatgaa
    31021 acttcaaaat acggaattat caatccagga gaagtattgg aagatgggtt atacaatgtc
    31081 aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa gtgatttagc tattattgga
    31141 cgttacttat tgactccaga aatttttgat gtattgtccg agcaacaacc aggagccgga
    31201 aatgaaatcc aactaactga cgcaatcgat acattgaata aaacacaacg cgtatttgcc
    31261 cgtaaattca caggtaaacg ttacgatgtc ggtgacaaat ttggttttat gaaaacaagt
    31321 attgaatatg gtctaacaca tccagaagta ggtgcacctt tacgtgagta catcctgtct
    31381 ttaggtaaaa cattagaagc agaaggaagc aagatcgatc ataatccaaa aggaataaca
    31441 gaaatcaaag aagaaaagaa aaaataaccc ttttttctaa cgagcttcag ctgctaattg
    31501 aataaaaagt agttgaagct cgtttttatt tgtttaaaaa acacgaaaat gctatcctta
    31561 tagaaaagag agacaagaag ggagtttgat catgtcagga ggagaaattg caggattgat
    31621 cgcagcaatc gcctttgcga tacttgtcgt ttttatcgtt cgtgtgttgt tgcaagtttc
    31681 taaaacttta gaaaaagtag atcagactgt agcagaagca aatacgacga tcgaaatcgt
    31741 cacaaaagac gtagatcttt tgtctagaca agtagaaggc ttactggtca aaagtaatga
    31801 actgctcttg gatatcaatg gaaaagtcgc aacgatcgat ccattgttca cagcagttgc
    31861 tgatttaagt gaaagtgtat cagaacttaa tcattctagt aaaaacatcg cgacgaaagt
    31921 cggcggaatc ggtaaaacga cagctaaagc gacggttgcc ggaaaagtcg gtgggacagc
    31981 gatgaaattt tttaaaaaca aaaaacatac tgaaacgaca ggaggaaaat aaaaatggct
    32041 aaaaaaggtg gatttttatt aggtgcagta gtcggaggca cagccgcagc aatcgccgcg
    32101 ttattgttag caccaaaatc tggtaaagaa cttcgggatg aattagctgc ccaagcggat
    32161 gatttgatgg atatggcttc agattacaca gatatggcga aagaaaaatc gactgagtta
    32221 acaggtattg caaaagaaaa agcgcaagat ctttctcaac aagcaggtga tctagcaggt
    32281 agtgtcaaac aaaaagtaag tgaatcagca gatgaagcag gaagtgaatc tgacgatatc
    32341 ttgttaaatc ttaaagaaca atcagctgac ttatctaatc gtttcaaaaa aacggcagaa
    32401 gatatgaaaa aacctgcaga tgaattagga agtattgcta aagatacttc agaagatatc
    32461 atgattgatg taaaagacgc tgctggtaaa gcaggagata ctgtcaatga aggaatgtca
    32521 gaagcaaaac cagttgcaga tgactcaaaa gaaatgatga aaatggcaaa agaagatgcg
    32581 aaagaagcta aaaaagaaat aaaagataca atgacagatt catctacaaa tcactaaaat
    32641 cacaaattta tagaataaaa aggtgggagc ggactagctc tcaccttttt tactcttatt
    32701 tttctaaaac aagtaattct tttggtgtag tcgtgaatgg cacacagcca tcgcttgtca
    32761 catatacaca gtcttcgata cgtaccccta ctttttcagg aatatagatt cctggttcaa
    32821 gagagaagca catcccttct tcgattacta agtcatttcc ttctacaagg gaagggaatt
    32881 cgtggacggt tgttccgatc ccgtgaccta aacggtgatt gaaatattcg ccgtagccat
    32941 agctgttgat cacgtttcgt gcgatttgat caagttcacc tgctgtcaca cctggacgga
    33001 cggcttcttg tgcggcaagc tgtgcttcaa gagtgatgtt gtagattttt tcttggaagt
    33061 cggttggttt ttgataagca attgtacgag tagcatcact gcaatagcca ttccaaacaa
    33121 cgccaagatc gaataacaca agttcattcg gtgaaatctt cgtatttcct ggtgtaccat
    33181 gagggcttgc agcatttgct cctgcaagga ctaatgtgtc gaaagacatt tgtgaaacac
    33241 cttgtttttt caattggtat tcaatttctg caatgatcgc ttgttcagtc acgccttctt
    33301 tcactgcttt aaagccaatc tcaaatgcaa catcggccca tttaccagct tcgatcagtt
    33361 tgtctttttc ctctagagtt ttgatcagtt gtagacgttg gatgatggaa gttagatcag
    33421 tgtaagaatc cgttcccgtc aagatatcag agatagcatc gaagcgctcg acagtcaagg
    33481 cgttcttttc aatccctaca ttgccaggct gaccatagcg agcacgaagt tcttttccga
    33541 tgatctccca agggttttcg ctgtctaaat agccacggac atcatacgcc caactactgt
    33601 tttgagcatc ttcaacttcc aaagcaggag tgaaaaggaa aggatcttgt tcaagaggga
    33661 taaaaagagc tagtacacgc tcatgaggat cactttcgta tccggaaaag taagcgatgt
    33721 ggccagggtc gctgatataa gccaattcga cttggtgttg tgccatccat tgacgtagtt
    33781 cattgatttt tacgttattc atttggcaac ctactttcta aattaatttt gtattcaata
    33841 attgtaccac tgatttatta aaagaccaac gttctaaaaa ctgaaaaaag taaaaatttt
    33901 catgaaaaca gagataaaac ggttgcaatt cttgagatta tttgatattc tattttagaa
    33961 atgtaagacg tattttcaga aaacatataa aatatgaata ggagaacaca aatggaaaag
    34021 caaacaatca ctatctatga tgtagcgaga gaggcaaatg tttcgatggc tactgtttca
    34081 cgtgtcgtaa atggcaatcc aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtt
    34141 atcgatcgct tggattatcg tccaaacgca gttgcgcgtg gcttggcaag taagaaaaca
    34201 acaacagttg gtgtaatcat ccctgatgtc agcaatatgt tctttgcttc tttagcacgt
    34261 ggtatcgatg atgtggcaac aatgtacaaa tacaatatta tcttagcaaa ttcagatggc
    34321 aacgaccaaa aagaagtcaa tgtattgaat aacttactgg ctaagcaagt ggacggcgtg
    34381 atcttcatgg gtcaccatat cacagacgaa atccgtggag aattttcacg ttcaaaaact
    34441 cctgttgtat tagctggctc tattgatcca gatgaacaag taggtagcgt aaacattgat
    34501 tatgtatccg caacaaaaga tgcagtaaac aaattggcaa aaagcggcaa taaaaaaatc
    34561 gcttttgtca gtggtgcgtt gatcgatccc atcaatggac aaaatcgtct gaaaggctat
    34621 aaagaagcat taagcgaaaa taacctacca tatagcgaag gattgatctt tgaagcacaa
    34681 tacaacttca aagatggtct atctctagtc gatcggatcc ataatagcgg tgcaacagct
    34741 gtctatgttt cagatgatga attagccatc ggtattttgg atggtttatt agaccgtggc
    34801 gtgaaagtac cggaagaatt cgaaatcatc acaagtaaca attcactact gacagaagta
    34861 gctcgtcctc gtttaacaag tatcacacaa ccattatacg atttaggggc tgtatctatg
    34921 cgcctattga cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct
    34981 tatggaatcg aagaaaaagg atctacaaaa taataaaagg acaaccattc agaggttgtg
    35041 tgcctgccgt ttaactctgc gttacaagga gcaatttcaa aaaacagcat ctcatgtttg
    35101 atgaatattg tgactcgtga ttcggagttg gcaggagaac accgtttatt tgggaaaagg
    35161 ggttatgaca aaattttgtt ataatccttt ttttgtgtct taaaaaagag agaaagtggt
    35221 tgaaattaca tttatcgtat ataaaacaat ccgtgtatag atgctgcatt ttgttcacca
    35281 ccatcaaaaa aataagggac tgtgacaata ctattgtcac aatcccttat tactgtgtat
    35341 tctattaaat caatcatctt tctcatctga ttctttagtt gcttgattcg ctcttgcata
    35401 agctgaagcg gtgttccagt aatctgtata gttttcatcg gtaccgccaa cgccaaattt
    35461 aaaggcgctt ttcttcggtc catttttcgc ccatagcgat tcaactgttt ttcctggtgt
    35521 ttggatggat tttttattga cagtaacttt tccaggtgtt tgaccagtaa atccagatac
    35581 ttttttcttc atcacatcag atgataattc gaatttctca tcagtgccaa aaatgtcagg
    35641 ttttgcttgg taagcttgat tgatcagatt ggcaagataa ttagccgaac gtatccttgc
    35701 tttgctgtcc attccagtat tgtcatcgtg ccctgcccag ctactgatgg taatagacgg
    35761 agtagagaca attagccatg aatctcggta ttcattggta gaaccagttt ttccgaccca
    35821 atctgctttt cctagatttc cgtttaagct tgagatggca tctttgaatg gtgtagtgat
    35881 ttttgcattg atcacactac gcatcatgtc attcatgatc gatgccgttg cttccgaata
    35941 gatccggaca gagttgcttt cgtgtttata aattacattt cctgcgttat ctgtgatgga
    36001 atcgatgata tagccttctt catagacgcc gccgtttgct aaagcctgaa atccgttggt
    36061 ctgttgaagc gtcgtgacat ctactgtacc taaaggagca gattcaacgc cccaattgtc
    36121 atttgccggg tagttcatct ttgccaagta gtgctcgtaa gcatattgtt tcgatccacc
    36181 ggaattcaac acatcttgat ataagtgata agccgggata ttgttggacc attctagtga
    36241 ctcacggacg gtttggaagg tgttcgatcc ttcgttagta gcattgacga ttttctcgcc
    36301 agcattttct ccttcttgcc aagttgttgc ataatctgat acacgggatt ctgatccgat
    36361 aagccccatg tcgattgcag gtccataaac aagaactggt ttgattgatg agccagcttg
    36421 acgttctgta tcgaaggcat ggttattttg attctgactg taattccgtc cgccaacgaa
    36481 tccgtagatg cggccagttc tgttatccat caatacattt ccggtttcta cttgactgcc
    36541 agcaccatca tctaacatgt atccataatt ttcaacgcca ttttgcaatg cagcatagac
    36601 atctttatcg attgtcgaat ggattgtata tcctttattt tggatcgttt gctgtgcctt
    36661 ttgataataa gcgtcactga ccgatgaatc agacatatca gcgttgtctt tttcagccaa
    36721 ttgttgtgca atgattcttg ttgcttcatt catcacagta taatacaaga attctctgtc
    36781 agtctgttct gcgatttgtt gtggtagaaa gtcttttgta aggtcatagg cttttgcttc
    36841 ttcgtattgt tctttcgtaa tttggccttc acgatacata ctgaataaaa caaaatcttt
    36901 tctggcaagg ccggcagaaa gatcttcttt tagcgcgcct gtgttcgtgt atggactata
    36961 cgtgatcggg ctttggggta atccagcgat ataagcagct tgcggcagcg tcacatcttt
    37021 tgcatctaca ccaaagattc cttttgcagc ttcctggact ccagcaatgt tttgtccctt
    37081 attattacga ccaaatggtg agacattcaa atacgtagca atgatctcat cttttgaaaa
    37141 gtatttttct acttgtgcag aaagaatgat ctcgtttgct ttacgtttga atgttgtttc
    37201 atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa gttgatccac cagaagagcc
    37261 aataccagta gcttcagata ctaatgctcg taaaacagct ttaggaacgt aaccttggtg
    37321 tttatcgaag tattcatcct ctgtgctgat aattgcagtt ttcaataaag gtgaaatctt
    37381 atcagatgaa atagtcgtgc gcatcagatc tgtctggatt tttgatatct ctgtattatc
    37441 tgcataaaca atctttgacg tttctgtaat gtttcctagt tctgtctgca gtgctttttt
    37501 agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag ccagcgccta ttccaatacc
    37561 tagagctgcg aacatcaaaa gaagaacagt aataaaaaca gtcaatgatt gaaaaactct
    37621 aaaaataatc ttaggaacaa accatttttc tttttttctt ctattctgtt tttttcgagt
    37681 atttttgcgt ggcaaaaggg tcacctcaac taatggtttt atttcgctaa ttatagcaaa
    37741 taatagaaca aaaaaacaga caaggctgtt tttggcagga atttcttttt taaaagagtt
    37801 aaaacgtgag aaaatgatct ttattataaa agagttctta agcttttctt tagagaaaat
    37861 ttcagttact atggagaaca gggcaaagaa aaatcaatca ttttctttgt atatcatctt
    37921 accaatatat aggaattgtt agtaacaatt gcctttcaag gaggaatcca tccatgttat
    37981 taggctcaca cgtcagcatg agcggaaaga aaatgctgtt aggagcagca gaagaagcag
    38041 ccagttatgg atcttcgact tttatgatct atacaggcgc ccctcaaaac actagaagaa
    38101 aagccatcga agagatgaat attgatgccg gaaaagcgta catggcagaa aaaggtctaa
    38161 caaatatcgt cgttcatgcc ccttatatca ttaatttggg gaatacgatc aaaccagaaa
    38221 tgtttccttt tgccgtccaa tttctgagag aagagattgt tcgtgcggaa gctttaggcg
    38281 ctactcaaat tacacttcac ccaggtgctc atgtaggggc aggaacagaa gccggaattg
    38341 ctagaattat tgaaggcttg aatgaagttt tgacaaaaga acagacggca caaatcgctt
    38401 tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac atttgaggaa ttagcgcaaa
    38461 tcatcgatgg agtaaaattg aatgaaaaat tatccgttac ttttgacacc tgtcatacga
    38521 atgatgccgg ttataatatt aaagaagact tttcaggtgt aatggaagag tttgataaga
    38581 tcatcgggtt agatcggctt aaagtactcc atatcaatga ttctaaaaat ccgcaaggtt
    38641 cgcataaaga ccggcacgcc aatattggtt ttggaacaat cggattcgac acgctgaata
    38701 agatcgtcca tgatccaatg tttgacaata tttcgaaaat cttggaaact ccctatgtag
    38761 gcaaagataa aaagaaagct tacgcaccat atggaaaaga aatcgccatg ttcaaagcgc
    38821 aaacattcga tccagaagtg tttcctgagt tattagcaga agaagtgata ctctaagatt
    38881 ttctatctga cagcagcaaa aaaggaccag gcacattgcc ttgtcctttt ttgtttaaga
    38941 aacaaaggag tactttttgc agtctttttc atcatgcatc ttctcccccc tctttttaaa
    39001 tttagacaaa caaggtatac tggtagaaaa gtaaaaaaac gaaaagtttc acaagcaagt
    39061 ggcaaaacgt gattggaagg acgaaaagat gctgaacact ttaaaaccct tgatcaacca
    39121 actaaaaaag aaacatacat actttattct tggcatagcc ctattattta caattattcg
    39181 tttgtattta tatagtaaag caatctatgg aacagacatg tcggcaacct atgatgacca
    39241 gctgctgatt cgctatgctc aaaatatttt ggacggtaaa tggctagggg agtatacgac
    39301 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
    39361 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
    39421 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
    39481 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
    39541 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
    39601 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
    39661 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
    39721 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
    39781 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
    39841 agttggcttt tccatcgtct atcaatggat cgaatataaa aatgagacaa catacggtat
    39901 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
    39961 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
    40021 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
    40081 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
    40141 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
    40201 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
    40261 aacaatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
    40321 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
    40381 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
    40441 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
    40501 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
    40561 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
    40621 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
    40681 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
    40741 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
    40801 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
    40861 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
    40921 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
    40981 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
    41041 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
    41101 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
    41161 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
    41221 ggtcatcatc agtaaaggaa tcatcgaaaa cggcaacgca aaagccaaga acacctggga
    41281 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
    41341 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
    41401 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
    41461 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
    41521 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
    41581 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
    41641 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
    41701 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
    41761 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
    41821 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
    41881 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
    41941 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
    42001 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
    42061 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcaatatc
    42121 tgttgccata atggctaatt cagtcaagat ccataaaatg acacctaatg tcttacttgt
    42181 gcgtgctcgg attgcctgtg ccaaatccat ttggctcacg attcctaatt ttgctgccat
    42241 atactgtaaa agcatagcaa tcagactaga catcaaaata actgacatca ataaatattg
    42301 gaaattttgt cctccggtga tcgaagtaga ccagttaccc gggtccatat acccaactgc
    42361 tactaacgca ccaggtccag agtaagcaag aagtgtttta aaaaaacttt tcccctttgg
    42421 tacatcaact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
    42481 atgcttatga aattgtttat tggattgatt attcaagaaa aaaccacctt tcctatttat
    42541 ctacgattac attctacacc tcaaaaagaa aaaattaaag aaaaagattc ggtacgccta
    42601 attttttttc atggcgcgtc ttgctagatg acgaaagaaa ttttgttctc ctctaaaaaa
    42661 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
    42721 aaataagtca ttgagagcaa ttcggaaata gaaaggagag atttatatgt tatcaacagg
    42781 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
    42841 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
    42901 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
    42961 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caacgacttt ttacgggctg
    43021 attttttcag tccgtttttt atttcgtaat ttgttagact attcagatta ttcagcaaaa
    43081 aagacaagca cagacaatcc taggctcttg ataaagacaa aggaggagta aaatgtcgat
    43141 ttttagaaaa ttaggctggt tttttcgtca ggaaaagaaa cattatttaa ttggggtttt
    43201 ctcgctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
    43261 tgatgagatc gccgataaaa atttatcaat aaagatcatt ttcgggtgga tcttcctatt
    43321 gattatcgct gcttttttac aatatctttt tcgttatatc tggcggacga atatttgggg
    43381 aagtgcagct cgattggaga aagaacttcg gcagcagtta ttcaaacatt ttacagaaat
    43441 ggacaatctc ttttaccaaa aatatcggac tggggatttg atggctcacg caacgaatga
    43501 tttgaatgcg atccagaatg tagcaggagc tggtatcttg acctttgcag attcagtgat
    43561 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
    43621 tgcgctgatt cctctgcctt tgctggccgt cacttcacga gtgctggggt caaaactcca
    43681 tgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
    43741 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tagcggattt
    43801 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
    43861 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
    43921 tggaaagtat gtcttggaag gcacgatctc gatcgggcag atggtctcat ttattagtta
    43981 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
    44041 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
    44101 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
    44161 taattatccg aaagaagaat caccagcatt agaacaaatc aagtttgcga tccaagaagg
    44221 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacgatca tcaaattact
    44281 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
    44341 atattcattg aatgctttat taggatcaat cggctatgtg cctcaggatc acttcttgtt
    44401 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
    44461 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattac caaaaggtta
    44521 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac aaaggatttc
    44581 gattgctcga gcattgatca aaaatcctga attattaata ttagatgatg cactctctgc
    44641 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
    44701 aacaactatc attgcagctc accgtttaag tagcgtgatg catgcaaaag aaatcattgt
    44761 tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat gagctgcttg cactaggcgg
    44821 ctggtatgaa caaatgtggg agaaacagca attagaagcg aagatagaag gaggcgaggc
    44881 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaaa
    44941 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
    45001 atcttatttg cgtttgcttt gtctgtgatc aatgtcctat tgccaaggat catccaaact
    45061 tttatggatg atcatctggc taaacagtcg gctacgacac aagttatttt gttttttgct
    45121 ggtatgtact tatttggagt catagtaaaa agtatcattt ggtttttcca atggtatttg
    45181 tattccatgg cctcattgaa gacctatcag tatattaggg taaaattatt tgaaaagctg
    45241 catacactag gtatgcgtta ttttgatcag acaccggcag gatcaaccgt ttctcgagtg
    45301 acgaatgaca cagaaacgct atttgaattt tggtacgtct ttttgatggt attgactggg
    45361 atctttgcag tcgtttcttc attccttgcc atgtttcaaa tcaatggaga aattgccttg
    45421 tggactttgg tgtttttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    45481 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    45541 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    45601 tttgaacaag tgaacaatga gtatttagac acgcgttatg cgatgatcaa aacgaattcg
    45661 attctgttaa gtccgatcat caattttcta tatgcattgg cgattgcgtt ggtgttgact
    45721 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    45781 acctatgtcc agggattttt taatccaatg tctcaaatga tggacttttt gagcatcttc
    45841 acagatggga tggttgctgg tagtcgtatc ttgaagatta tggatacgga agaatatgca
    45901 ccacaacaaa accctggagc agatcaaatg attacttcag gtaagattga gtttcgcaat
    45961 gtaagctttt cttatgatgg agagcacaat gtattaaaca atatctcttt tgttgctaat
    46021 ccgggagaga ccgttgcatt agtgggacac accggaagcg gaaaaagctc gatcatcaac
    46081 gtattgatgc ggttttacga attttatgaa ggtcagatat tgattgatga tcatgacatt
    46141 cgagaatatc caatggaaga gttacgtaag aaaatggggc tggtcttgca agatgctttc
    46201 ttgttttatg gggatatcgc aggaaatatt cggatgatga atccagctat cacagacgaa
    46261 cagatacgcc atgcggctga gtttgttcaa gcaaacaaat tcatcgatga attaccaaaa
    46321 ggatatcact caaaagtgat cgaacatggt gccagctatt ctagtggaca acgacagctg
    46381 atcacttttg cgcgaacaat cgtgactgat cctaaaattc tagtattgga tgaagcaaca
    46441 gcaaatatcg atactgaaac agaaggattg atccaagaag gattggcaaa aatgcgtcaa
    46501 ggacggacaa cgattgcgat tgcccatcgg ctgtcaacga ttcgagatgc agaattgatt
    46561 ttagttttag acaaaggtca tatagtagaa cgcgggaatc atgaaacact tctagcgcaa
    46621 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtatataaaa gatgttgtga
    46681 cagtagaatt ttgacctaag cactagagtg aaaataaaac atagcttctc atatttttca
    46741 tctgtgagag ttagtgtcgt agtcaccaga tttaaaaaaa gattcgacag ctagtcagaa
    46801 gtgcctaata gccactaaga aagcgttgac aaaagcattt gctttcgata atataaagaa
    46861 aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag tctcccatgg
    46921 tttcatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta tacatcatgg
    46981 aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa taaccaggga
    47041 tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa agaaagtggg
    47101 tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg acgggctttc
    47161 acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa tttcttgtga
    47221 aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg tgtatattgc
    47281 attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat ttgtaccaaa
    47341 tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg ggaaaaaaac
    47401 agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag ccggatttat
    47461 cgcattgcat ttagtcaatg cacaaggcat tcaaagccag caagacaata cctacctgtt
    47521 tactataaca atacaggata ttctaaatat cgtccgctat tactataaga ttacatttga
    47581 tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc tgacacgcgt
    47641 cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc aggttattca
    47701 aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt tagccgagac
    47761 gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata ctgcgcgttt
    47821 agtgcatgag aatcgttgta aataaaaaaa ggaggataaa ttaacaagaa cgtatgaaat
    47881 aagggtagtg acattcagtt ggctaaacct tccactaata aattggaggg aatagaaatg
    47941 ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg tcggcgggaa ggaaaatatc
    48001 aatggtttga cacattgtat cacaaggctt cgtttcaaat taaaaaacga agaaaaagca
    48061 aatgatgaga tcttgaaaaa tatggacggt attgtaacgg taatgcgagc gggagggcag
    48121 tatcaagttg tcattgggaa tcatgtgcca gtcgtgtttg aagaggttat aaaagcagga
    48181 aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga gaccgtttga tatgcttatt
    48241 gatggaatat cgggctgttt ccagccattc cttgctatcc tagctgctgg cgggatgatt
    48301 cgtggactga cagcattcct tgtcttctta ggcgcgtttg accgcggttc tggcacattt
    48361 gttatgtttg ataatatcgg tgattctgta ttccaattta tgcctgttat tatcggttta
    48421 acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga tgttgcttgg cgcagcatta
    48481 atgaatccta gcttgtcgct ggaagctttg agtggtgcag cagaagcacc actaacgacc
    48541 atattttctg gcactatttt tgaagcacct atttatcaaa cagtattcgg cattccctgg
    48601 attgcgcgta actacgcttc ttctgtcatt cccattatct ttattgtgtt gttagctagc
    48661 caaattcaga aaccaattaa aaagttagta cctgagatga tcgcgaattt cttcgttcca
    48721 ttcttcaccg ttcttattac aatgccttta ggttttttac tcgttggacc agtattcaca
    48781 ttcgcaactg atattttaat ggctggattt gaaacacttt tagcactttc gccagttatt
    48841 tatggagcaa tcgtaggctt cttctggcaa attctggtga tgtttggtct ccattgggca
    48901 atcgtaccaa tgggattgat gcaattttct gtgaatggtt ggcaaaatat tatgacacct
    48961 gttgcagtag tgtcttttgg acaaactgca gcacttaccg cactctactt taagcttaga
    49021 aatccaaaag acaaagcaat tgcaatacca gccattgttt ctggtattgt ggggattaca
    49081 gagccagcta tttatgggtt tacattacca cgtaaaaaaa tatttatttt tacctgtgtc
    49141 ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt taacttcttg gaatcaaggt
    49201 ggactaggca tctttacaat tcccaactat attagaccag atggtgattt aacagatgtg
    49261 attaatgtat tgattgggat cgccattgcg atggtggtat cctttacgct gacttttttc
    49321 ttttggaagg atgaagcagg tgaaacagat gatatacaga aaaaatcagg taaagaaatt
    49381 gtaaaaacac cgatacaagg acaaatcgct ccactaaatg cagcaaagga tgcagctttt
    49441 gcacaaggaa cactaggtcg aggaatcttg atctaccctg aaaaaggaga agtacgtgcc
    49501 ccttttgacg gaacagtcat gaccctgttc ccgacgaaac aggcaattgg aatggtttct
    49561 gaaacagggc tggaattgct gattcatgtc ggattggata ctgtgcagtt ggaagggaaa
    49621 tattttgaaa gcttagtgca gcaaggataa agagtgaaac aaggcgatct gttgctacgc
    49681 ttcgacaaaa agaaaatcga aaaggaagga tacagtttag aaacacctgt gatcgtcacc
    49741 aattacacgg attatttgga tattttggaa aaccaagggg agacggtcgg gccagacgat
    49801 gcacttatca ccgcactgac ctgaacggtt aggtgaagaa tggactaggc ttaactattc
    49861 tttttattcc acttctttat tttcaaaatt gaaaggctct ttcatgatgg ctgagctgat
    49921 agggcgttta tcgaattttt gaccgacaga aattagatgg actaaattct gttgataatt
    49981 ttctgcaaaa acacaactat ataacacgtc tagaagataa caaattgatg tattgatcgt
    50041 aaaattacca atttttgaat acagtcgctc gcgtgtcgta agtcgcaaag cggctgtact
    50101 cattgcagat agcgttgaat caccgatact ggtcaaagct aaaattggaa catgattttg
    50161 tttcaatatt tctgcggtac gaaggatcac attgttttca cccgtataag aaatcagaat
    50221 cccgcacata tcactcgtgc aattgtacgc ttcatagaat gattcaccaa aagtggtgac
    50281 aatcgtgaca tccattttga tgcgcttcat tttcagcatg aaatcttgag aaatcaaagt
    50341 attcgagtta ctggcgaaaa tcacgatttt ttttgcttga agcattagtt gtttggcttt
    50401 ttgcagttgg tcatgatgaa tgagagaaag tgtatcatga atcgtcatct gttctaaaga
    50461 agccatttta tttgcgatcg tcataatacc gtctgtggtg gaaaaaggaa gattcgcatc
    50521 tacgtcttga aaattccctt caagataggc tgtttcagct aaaaaagcat tacgaaaact
    50581 gacccagcca tcaaaaccca ttttttttgc tacacggatg agtgtagagg ggtggacata
    50641 acatcgctca gcaatcgctt ggatggtcat ttcgccaagt gctggagaat cgcttaagag
    50701 aaactggatc acttcatttt ctgaaggaga aaaggagatt tctttgattt tttcacgtaa
    50761 taacatgtag ataccttctt cttatttttt tacgtaaatt ataacttttt tgaaaatgat
    50821 ttgtaaacgg atttatacta tataacttaa aatagacatt ttttgacaag atatttacaa
    50881 aaatccacta gaaaacagta aattttgtaa atgttactgg aaagaaacag atttctattg
    50941 tttgttttgt ttacaaaaaa gataatgaaa gtgtaaaaaa gtaaaggagt gagaacatgg
    51001 caaaaggaat caaaatcgct acgattggtg gaggcagcag ttacaccccc gagttgatgg
    51061 aaggatttat caaaagatat gaggaattac cgatacgaga aatctggctc gtagatgtag
    51121 aagaaggaaa agaaaagctt gagatcgtta gtcagatgtc tcagcgtatg tgggatgctt
    51181 ctccgtatga agtcaagatc tatgcaactc tggatcggga agaagcattg aaagatgcgg
    51241 attttgttac gactcagttt cgtgtcggtc aattagatgc acgaatcaaa gacgagcgga
    51301 tccctagtta ttatgggatg attgggcaag aaacaaatgg tgctggcggg atattcaaag
    51361 cttttcgtac cgttccagtc gttttggcaa tcgttgagga catgaagcgt ttatgtccaa
    51421 atgcttggct gatcaatttc gcgaacccaa gtgggatggt gacagaagca gttatcagat
    51481 atggcaaatg ggataaagtg attggattat gcaatgtgcc agtgatggcg atgatgacag
    51541 aaccagcaat gcttggcaag aaaaaagaag agctgatcta caagtttgcc ggtctaaatc
    51601 atttccattg gcacaaagta gcagataata aaggaaatga ccgcacggca gaattgattg
    51661 atcgtttata cgatgaagag aatggattac cgaaaaatat ctttgatatc ccgtttttca
    51721 aagaacaatt aaaacaaatg aacatgatcc cttgtggcta ccaccgctat tactatcgtg
    51781 aagaagaaat gctggaacat gctttagaag aatatcaaac gattggtaca agagcgcagc
    51841 aagtgaaaga aacagaagca gagctatttg aattatacaa agatccagaa ttagaccata
    51901 aacctgaaca attgcaaaaa cgaggtggcg cttattattc agacgctgcc tgtgaaacga
    51961 ttgcttcaat ctatggaaat aaagaaacac agatcgtggt atctacaaag aatgatggag
    52021 ctgttcctga cctgccggca gattgtgtag tagaagtcac tgcctatgtt ggcgcacaag
    52081 gagcgagaaa tgtagcgttt ggttctcttc ctgctgctga aagaggctgg ttgcaagtaa
    52141 tgaagaatat ggaattgctg acgatcgaag cagctgtgac tggtgattat gggacagctt
    52201 tacaggcgtt tacgatcaat ccacttatac gatcaggaaa gacagcaaaa cggatcatgg
    52261 atgagttgtt tattgcacac cagcagtatt taccgcaatt caaggagaca atcagccgat
    52321 taaaagaaga aggaatcgaa gttcaagatg aactggcaag agaattatca ggtaatacac
    52381 atataatgga ggaagtataa tgggattcag aaaagatttt ttatggggcg gtgctacagc
    52441 agccaatcaa tgtgagggcg gttatgatga aggtggacgc ggactagcaa acgtagatgt
    52501 tgtgccaatc ggaaaagacc gttttcctgt tatcaccgga aagaaaaaaa tgtttgaatt
    52561 tgatgaggat catttttatc cagcaaaaat tgcaatcgat atgtatcatc gttataaaga
    52621 agatattgct ttgtttgcag aaatggggtt caagacttat cgaatgtcca tcgcctggag
    52681 tcgtattttt ccaatggggg atgagaaaga gccgaatgaa gctggcttga aattttatga
    52741 ggatgccttt aaagaatgcc aaaagcacgg aattgagcca cttgtgacga ttacgcattt
    52801 tgattgtcca atgcatttga tcaaagaata tggtgcgtgg cgttccagag aactcgttgg
    52861 tttttatgaa aatctgtgtc gagtcatctt caatcgctat aaaggacttg tcaaatattg
    52921 gctgacattc aatgagatca atatgatcct tcatgcacca ttcatgggcg cagggctgta
    52981 ttttgaaaaa ggagaaaatg aagaacaaat caaatatcaa gcggcacatc atgagttggt
    53041 tgcaagtgcc atcgcaacga agattgctca tgaagtagat ccggagaaca aagtaggctg
    53101 tatgctggca gcaggagcat attatccata cacttgcaat cctgaagatg tttgggcttc
    53161 tagaaaatca gatcgagaaa attatttctt cattgatgtc cagtcacgag gagaatatcc
    53221 cgcttatgca ttgaaagaaa tggagcgaaa tggcattcag atcgaaatgg aaccagggga
    53281 tgccgcattg ctgaaagaac atactgttga ttttatctct ttttcttatt atgcttcccg
    53341 agtttcgact accgacaacg aactcttgga acagactgca ggaaatatct ttgcttctgt
    53401 caaaaatcca tatctgcaag caagtgaatg ggggtggcaa attgatccat taggcttgag
    53461 aataacgatg aatgatattt atgatcgtta tcaaaaacca ttgtttattg tagaaaacgg
    53521 cttaggtgca gtcgatacgc cagatgaaaa tggttatgtg gaagatgatt accgaatcga
    53581 ttacctggca gcgcatatcc aggcgatgaa ggatgcagta gaactagacg gagtcgatct
    53641 attaggctat acgacttggg gatgtattga tttagtatca gctggtaccg gtgagatgaa
    53701 gaaaaggtac ggattcattt atgtcgatcg tgataaccaa ggaaatggca cattagcccg
    53761 ttcaaagaaa aaatcattca actggtataa aaaagtaatt gattcaaatg gagaaaatct
    53821 ttcgaacgat tgatcaatga tttataaaag tctattgttt ttcagcggat ttttcgagta
    53881 taataaagct attcgagaaa cgcaagcaga acaatagact ttttttcgca ttggctgtaa
    53941 agtatgctta cttgattaag taaaggagaa tctacatatg tatgatgtaa tcgttattgg
    54001 cgcaggtcct gctgggatga ctgctgcttt atatgcttca cgttcaaact tatccgtatt
    54061 gatgatcgaa caaggtgccc ctggcggcca aatgaacaat acggcagaaa tcgaaaatta
    54121 tcctggattc gattcgatca tgggccccga tctagcttat aagatgtatg aaagtgtctc
    54181 aagatttggg acagaaaatg cttatggaat cgttcaagat atcaaggatc acggcagcca
    54241 taaagaagtc atctgtgaag ataaaagtta cgaaggaaaa acagtcatta tcgcaacagg
    54301 atgtatccac cgtaaattag gcgtacctgg cgaggaagaa tttgctggac gaggcgtttc
    54361 ttactgtgca gtctgtgatg gtgcgttttt ccgtaacaaa cgtctagtag tcatcggggg
    54421 tggggactca gcagtagaag aagccatcta tttgacacaa tttgcttctg aagtagtgat
    54481 cgtccaccgt agagatgaat tacgtgcaca aaagatcatc caagaccgtg catttgcaaa
    54541 tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa atcgttggaa acgaaatggt
    54601 agtgacaggt gtgaaagcac gtaatgtcaa aaccggcaat gaatttgatt tagcagcaga
    54661 cggagtattc atctatgttg gactggatcc attaactgca ccttttagag aagcaggttt
    54721 gacgaacgaa gctggctgga ttccaacaga caacgaaatg aaaacgaata tgccaggcgt
    54781 ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa atcactacag ctgttggtga
    54841 cggcggtatt gctggacaac aagtgtataa atacttggag tctttagaag aaacaacagc
    54901 ataagtttaa aaagagtaga agaacgggtt caattgttct tttactcttt tttttcgcgt
    54961 gaaatttact gaatctgatt tatgcgaaca cgtccaacac cttttgataa aagaatcctt
    55021 tcttgattta agcgtttttt ggtcctcata ctttcatatc taataagtga atgctatact
    55081 tattaagaac catatttcag aaagaggtgt tattttcatg tcttgggaac aagtgtatga
    55141 acaatgggta catgaagaaa atttagaaga aaacttaaaa aaacaattaa atgatctggg
    55201 tgaagatcct gaaaaacttg aagatgcttt ttatgcaccg ttggaattcg gtactgcagg
    55261 tatgcgggga atcttaggac ctgggatcaa tcgaatgaac atctatacgg taagacaagc
    55321 aacagaagga ctagcacgtt tcatggatac acaaggtgaa gaaacaaaac gccgaggtgt
    55381 cgctatcgct tatgactcac gccatcagtc accagaattt gcaatggaag cagccaaaac
    55441 actagcacat cacaatatcc cgtcttatgt attcgaaagt ctgcgtccaa caccagagct
    55501 ttcttttgca gttcgtcatt tgaagacgtt tgcgggaatc atggtaaccg catctcacaa
    55561 cccagcggct tacaatggat ataaagtcta cggagaagat ggcggacaaa tgcctcctgc
    55621 agacgcagat gcattgacga aatatgtccg tgaagtcagc aatccgctaa aagtcgaagt
    55681 attatctgac gaagaagcta aacatagcgg gttgatcacg atcatcggag aagaagtgga
    55741 cgctgcttat ctagaagaaa tcaaagcagt gacgattgat cgtgaattgg ttgaaacaat
    55801 ggggaaagat ttgaaacttg tgtacacgcc acttcatgga acagggaaaa tgttgggcga
    55861 acgtgcgttg aaacaagctg gattcgaaca attcgtacta gttcccgaac aagctgtggc
    55921 agatccagat ttcactacgg tcaaatcgcc aaatccagaa gaacattcag cttttgaata
    55981 tgctatacga cttggagaaa aagaaggagc tgacttgctg attgcaacag atcctgatgc
    56041 agaccgttta ggcgcagcgg ttcgtttgcc agatggttct tatgaagtat tgactgggaa
    56101 ccaaatcgga gcattgtttg ctcaatatat tttggaagca catcaatcaa gaggcacttt
    56161 accagaaaac gcagcggtat tgaaatcaat cgtttccagc gaattgccta cagcaattgc
    56221 ggaacactac ggtgctacca tgttcaatgt gttgacaggg tttaaattca ttgctgaaaa
    56281 aatccaacaa tttgaagaag accattcaca tacttttatg tttggattcg aagaaagtta
    56341 tggttattta gttaaacctt ttgttcgcga taaagacgcg atccaagcat tgcttttatt
    56401 agcagaagta gcagcttact ataaaaaaca aggcaaaacg ttgtatgacg gcttgcaaac
    56461 gatttttgaa acctatggct atttcgcaga aaaaacgatt tctgtgacaa tgagcggaca
    56521 agaaggttct gcgaaaatct ctgctttgat ggaaaaattc cgtaaagaag caccgacaga
    56581 atttgccgga gcaaaagtta ttcaaacaga agactttaaa gaattgacga gaacagaccg
    56641 tgaaggaaac gtagaaaaac taacaacacc gccatccgac gtattgaagt atacattgga
    56701 agaaaatggc tggattgcta tccgcccttc aggaactgag ccgaaaatca agttttatat
    56761 tggtgtaaaa ggaagttcta cagaagacgc agccgaaaaa gtagcctctt atgaagcgat
    56821 tatcaaaaag atgacagaag aataaaaaac ttgagactga tggaaaaagt ggtaaacttt
    56881 agaagtcagt cttttttcaa gttggaggtg aatggattgc cagacacgca aaaatcaaaa
    56941 gtagagaaag tcagtcaatt attcaaaatg ttgagtgatc ccactcgtct taaaattctg
    57001 ctatatctga aagacgggga acaaaatgtg acagcaatca cccaagcagt ggaaatggag
    57061 cagtctgctg tatcgcatca actacgttta ttaagagaaa atcatgtcgt caagtctcat
    57121 cgcgagggaa aagcgatttt gtatagtctt gatgattctc atgtactgga tattttgaac
    57181 cagacactca aacatgtgga acatcaataa cgataggtgc aaatagataa acactggagt
    57241 gtgacaacga agaaatttcg ttgtcacact ccagtgtttt ttaagaaaaa gacaaaaatg
    57301 atcaatcgct tttttctgtt gtttcagttc cataataact atgatcttct aggtctaaag
    57361 cattcaaaat aagcgaagcc gtttcttcaa tagataaaga tgctacattg atcactacac
    57421 atcctagttt ttcataaaga tcattcgcaa aatcgagttc agctctaatt ttttcgatat
    57481 cggaataagc agtatcaggg tttaatccgt aagagcgcat ccgttcttta cggatattat
    57541 ttaaaatatc tgggttattt gttagtccca cgattttttt aggatcgatc tcccaaagct
    57601 gtttgggaat atgtgcttgt gggatcaaag gtaaattggc aacttttaaa tttttgttag
    57661 ctaaaaacaa gctcaaaggc gttttggaag ttcgggatac gcctagcaaa acgacatctg
    57721 cttctaaaaa accacgagga tctttcccat catcgtattt tacagcaaat tccattgctt
    57781 tgatccgttt gaaatagttc tcattcaata aatggagggc accaggttgc cgaattggct
    57841 tgataccagt taaacgttca atttctgata tcggtggcgt catcacatca aaactaaata
    57901 attgatgttc ttcacaaaaa gaacgtgtta tctcgatcag ttcttgattg attaatgtat
    57961 gcaggacaag tgctttttct tctaatgcat cttgcaatgc ctgccttaat gttttttcat
    58021 ctgtcacgaa cgttctgcgg aataggttga actcaacggt aggatactga gccatagtag
    58081 cttgcgctag ttttgtcgca gtttctccag cagagtcgga gatgacaaaa aaagtaacga
    58141 tttctgtaga atcggaagtc atggataaaa cctcacttgc atttttttta tgtttattat
    58201 accatactct agaatgcgta cttcgtaatc attttgattt ggagggataa gtcatgaaaa
    58261 aacagacatt gatcgaacaa tcattagaaa cattgaaaaa taacggtttt aaatatacta
    58321 aaaaaagaga agcattgtta acttatttag tgaagcgtaa ccgctacgta tctgctaaag
    58381 aagtttttga ctatatgaat caggaattca aaggggtaag ttatgatacg atttaccgga
    58441 atctccatga ttttgcagag atcgatttac tggaagaaac cgatttaaat ggagaaatga
    58501 agtttcgttt tcattgctgt caggggaaaa tcggccatca ccatcatttt atctgtacag
    58561 tgtgcggaac aacgaaagaa atccatatgt gtccgatgaa ttatttcgaa gaacagctag
    58621 gtggatgcac gatcgaaggg catagattcg agattttcgg aagatgtgaa aattgtcaat
    58681 aaggatttaa ataaaataac atttgataat tttaaaaaaa agcaagattt ccttgacggt
    58741 caaaacagca ttagatataa tatttacagg acagtcttgt tttttgagat atatttcaat
    58801 aacaattacg gttacttttt aagctcggag ggagggaatt aacatgtcaa aaacagtcgt
    58861 tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc aaacgttccg tgtcaaaagc
    58921 gggtacttta caagaatctc gtaaacgtga attctacgaa aaaccaagtg taagacgcaa
    58981 gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag ttctgtgatg aattggagtt
    59041 ggtagtgtgt cactactaag tagattaaac gatgacatga aagcagcgat gaaagcgaaa
    59101 gataaggaat ctttacaagt catccgaatg atcaagtcat ctatacaaaa cgagcagatt
    59161 aaagaaggtc acgacttgac agaagaagaa gaactaacgg tgttgtctcg tgaaatgaaa
    59221 cagcgacgtg attctttgca tgagtttgaa gaagcaggac gtgacgattt agctgaaaaa
    59281 gtaaaaagtg aaattgtaat agtcgaaaaa tatatgccgg aacaactttc cgacgaagaa
    59341 attcgacaac ttgttcaaga agcaatcact caaactggtg cttcatcaat gaaagaattt
    59401 ggcaaagtta tgggtgcaat tatgcctaaa gtcaaaggaa aagcagatgg aaaccaagtg
    59461 aatgcgattg tgaaagaact attacaatct taattttatc atttaaactg cggacagtga
    59521 ttatccgcag ttttttgtgt tcgtaaaaaa agaact
//
LOCUS       DACDVT010000009        59214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDVT010000009 DACDVT010000000
VERSION     DACDVT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.009"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            171..509
                     /locus_tag="IWL52_000530"
     CDS             171..509
                     /locus_tag="IWL52_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777116.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            603..833
                     /locus_tag="IWL52_000531"
     CDS             603..833
                     /locus_tag="IWL52_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777117.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            1233..1604
                     /locus_tag="IWL52_000532"
     CDS             1233..1604
                     /locus_tag="IWL52_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9777118.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(1878..2570)
                     /locus_tag="IWL52_000533"
     CDS             complement(1878..2570)
                     /locus_tag="IWL52_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9777119.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(2573..3358)
                     /locus_tag="IWL52_000534"
     CDS             complement(2573..3358)
                     /locus_tag="IWL52_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9777120.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            3595..4995
                     /gene="lacG"
                     /locus_tag="IWL52_000535"
     CDS             3595..4995
                     /gene="lacG"
                     /locus_tag="IWL52_000535"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9777121.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5020..5337
                     /locus_tag="IWL52_000536"
     CDS             5020..5337
                     /locus_tag="IWL52_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9777122.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            5350..7023
                     /locus_tag="IWL52_000537"
     CDS             5350..7023
                     /locus_tag="IWL52_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9777123.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            7037..8668
                     /locus_tag="IWL52_000538"
     CDS             7037..8668
                     /locus_tag="IWL52_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9777124.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(9159..10724)
                     /gene="guaA"
                     /locus_tag="IWL52_000539"
     CDS             complement(9159..10724)
                     /gene="guaA"
                     /locus_tag="IWL52_000539"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9777125.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10783..11706)
                     /locus_tag="IWL52_000540"
     CDS             complement(10783..11706)
                     /locus_tag="IWL52_000540"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9777126.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11902..12507
                     /locus_tag="IWL52_000541"
     CDS             11902..12507
                     /locus_tag="IWL52_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9777127.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12815..13831
                     /locus_tag="IWL52_000542"
     CDS             12815..13831
                     /locus_tag="IWL52_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9777128.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13972..15273
                     /locus_tag="IWL52_000543"
     CDS             13972..15273
                     /locus_tag="IWL52_000543"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9777129.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            15319..15981
                     /gene="deoC"
                     /locus_tag="IWL52_000544"
     CDS             15319..15981
                     /gene="deoC"
                     /locus_tag="IWL52_000544"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9777130.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            16002..16400
                     /locus_tag="IWL52_000545"
     CDS             16002..16400
                     /locus_tag="IWL52_000545"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9777131.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            16466..17548
                     /locus_tag="IWL52_000546"
     CDS             16466..17548
                     /locus_tag="IWL52_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9777132.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17760..19322
                     /locus_tag="IWL52_000547"
     CDS             17760..19322
                     /locus_tag="IWL52_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777133.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            19315..20448
                     /locus_tag="IWL52_000548"
     CDS             19315..20448
                     /locus_tag="IWL52_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777134.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            20448..21404
                     /locus_tag="IWL52_000549"
     CDS             20448..21404
                     /locus_tag="IWL52_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777135.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            21503..22669
                     /gene="deoB"
                     /locus_tag="IWL52_000550"
     CDS             21503..22669
                     /gene="deoB"
                     /locus_tag="IWL52_000550"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9777136.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22693..23511
                     /locus_tag="IWL52_000551"
     CDS             22693..23511
                     /locus_tag="IWL52_000551"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9777137.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            23528..24235
                     /gene="deoD"
                     /locus_tag="IWL52_000552"
     CDS             23528..24235
                     /gene="deoD"
                     /locus_tag="IWL52_000552"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9777138.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(24533..25219)
                     /gene="gpmA"
                     /locus_tag="IWL52_000553"
     CDS             complement(24533..25219)
                     /gene="gpmA"
                     /locus_tag="IWL52_000553"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9777139.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(25391..25972)
                     /locus_tag="IWL52_000554"
     CDS             complement(25391..25972)
                     /locus_tag="IWL52_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9777140.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(26105..26788)
                     /gene="rpiA"
                     /locus_tag="IWL52_000555"
     CDS             complement(26105..26788)
                     /gene="rpiA"
                     /locus_tag="IWL52_000555"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9777141.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            27107..27520
                     /gene="rpsL"
                     /locus_tag="IWL52_000556"
     CDS             27107..27520
                     /gene="rpsL"
                     /locus_tag="IWL52_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9777142.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            27604..28074
                     /gene="rpsG"
                     /locus_tag="IWL52_000557"
     CDS             27604..28074
                     /gene="rpsG"
                     /locus_tag="IWL52_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9777143.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            28168..30255
                     /gene="fusA"
                     /locus_tag="IWL52_000558"
     CDS             28168..30255
                     /gene="fusA"
                     /locus_tag="IWL52_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9777144.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            30427..31614
                     /gene="tuf"
                     /locus_tag="IWL52_000559"
     CDS             30427..31614
                     /gene="tuf"
                     /locus_tag="IWL52_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9777145.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            32020..32328
                     /gene="rpsJ"
                     /locus_tag="IWL52_000560"
     CDS             32020..32328
                     /gene="rpsJ"
                     /locus_tag="IWL52_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9777146.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            32359..32988
                     /gene="rplC"
                     /locus_tag="IWL52_000561"
     CDS             32359..32988
                     /gene="rplC"
                     /locus_tag="IWL52_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9777147.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            33016..33639
                     /gene="rplD"
                     /locus_tag="IWL52_000562"
     CDS             33016..33639
                     /gene="rplD"
                     /locus_tag="IWL52_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9777148.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            33639..33929
                     /gene="rplW"
                     /locus_tag="IWL52_000563"
     CDS             33639..33929
                     /gene="rplW"
                     /locus_tag="IWL52_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9777149.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33968..34801
                     /gene="rplB"
                     /locus_tag="IWL52_000564"
     CDS             33968..34801
                     /gene="rplB"
                     /locus_tag="IWL52_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9777150.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34844..35122
                     /gene="rpsS"
                     /locus_tag="IWL52_000565"
     CDS             34844..35122
                     /gene="rpsS"
                     /locus_tag="IWL52_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9777151.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            35145..35492
                     /gene="rplV"
                     /locus_tag="IWL52_000566"
     CDS             35145..35492
                     /gene="rplV"
                     /locus_tag="IWL52_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9777152.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            35506..36162
                     /gene="rpsC"
                     /locus_tag="IWL52_000567"
     CDS             35506..36162
                     /gene="rpsC"
                     /locus_tag="IWL52_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9777153.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            36165..36599
                     /gene="rplP"
                     /locus_tag="IWL52_000568"
     CDS             36165..36599
                     /gene="rplP"
                     /locus_tag="IWL52_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9777154.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            36589..36777
                     /gene="rpmC"
                     /locus_tag="IWL52_000569"
     CDS             36589..36777
                     /gene="rpmC"
                     /locus_tag="IWL52_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9777155.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36802..37068
                     /gene="rpsQ"
                     /locus_tag="IWL52_000570"
     CDS             36802..37068
                     /gene="rpsQ"
                     /locus_tag="IWL52_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9777156.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            37126..37494
                     /gene="rplN"
                     /locus_tag="IWL52_000571"
     CDS             37126..37494
                     /gene="rplN"
                     /locus_tag="IWL52_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9777157.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            37531..37839
                     /gene="rplX"
                     /locus_tag="IWL52_000572"
     CDS             37531..37839
                     /gene="rplX"
                     /locus_tag="IWL52_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9777158.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37867..38406
                     /gene="rplE"
                     /locus_tag="IWL52_000573"
     CDS             37867..38406
                     /gene="rplE"
                     /locus_tag="IWL52_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9777159.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            38425..38610
                     /locus_tag="IWL52_000574"
     CDS             38425..38610
                     /locus_tag="IWL52_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9777160.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            38646..39044
                     /gene="rpsH"
                     /locus_tag="IWL52_000575"
     CDS             38646..39044
                     /gene="rpsH"
                     /locus_tag="IWL52_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9777161.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            39076..39612
                     /gene="rplF"
                     /locus_tag="IWL52_000576"
     CDS             39076..39612
                     /gene="rplF"
                     /locus_tag="IWL52_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9777162.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39773..40129
                     /gene="rplR"
                     /locus_tag="IWL52_000577"
     CDS             39773..40129
                     /gene="rplR"
                     /locus_tag="IWL52_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9777163.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            40150..40650
                     /gene="rpsE"
                     /locus_tag="IWL52_000578"
     CDS             40150..40650
                     /gene="rpsE"
                     /locus_tag="IWL52_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9777164.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            40665..40844
                     /gene="rpmD"
                     /locus_tag="IWL52_000579"
     CDS             40665..40844
                     /gene="rpmD"
                     /locus_tag="IWL52_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9777165.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40888..41328
                     /gene="rplO"
                     /locus_tag="IWL52_000580"
     CDS             40888..41328
                     /gene="rplO"
                     /locus_tag="IWL52_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9777166.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            41328..42623
                     /gene="secY"
                     /locus_tag="IWL52_000581"
     CDS             41328..42623
                     /gene="secY"
                     /locus_tag="IWL52_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9777167.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            42685..43332
                     /locus_tag="IWL52_000582"
     CDS             42685..43332
                     /locus_tag="IWL52_000582"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9777168.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            43523..43741
                     /gene="infA"
                     /locus_tag="IWL52_000583"
     CDS             43523..43741
                     /gene="infA"
                     /locus_tag="IWL52_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9777169.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43774..43890
                     /gene="rpmJ"
                     /locus_tag="IWL52_000584"
     CDS             43774..43890
                     /gene="rpmJ"
                     /locus_tag="IWL52_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9777170.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43908..44273
                     /gene="rpsM"
                     /locus_tag="IWL52_000585"
     CDS             43908..44273
                     /gene="rpsM"
                     /locus_tag="IWL52_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9777171.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            44301..44690
                     /gene="rpsK"
                     /locus_tag="IWL52_000586"
     CDS             44301..44690
                     /gene="rpsK"
                     /locus_tag="IWL52_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9777172.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44770..45708
                     /locus_tag="IWL52_000587"
     CDS             44770..45708
                     /locus_tag="IWL52_000587"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9777173.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45736..46116
                     /gene="rplQ"
                     /locus_tag="IWL52_000588"
     CDS             45736..46116
                     /gene="rplQ"
                     /locus_tag="IWL52_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9777174.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            46388..47227
                     /locus_tag="IWL52_000589"
     CDS             46388..47227
                     /locus_tag="IWL52_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9777175.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            47203..48072
                     /locus_tag="IWL52_000590"
     CDS             47203..48072
                     /locus_tag="IWL52_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9777176.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            48062..48859
                     /locus_tag="IWL52_000591"
     CDS             48062..48859
                     /locus_tag="IWL52_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9777177.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            49062..49808
                     /gene="truA"
                     /locus_tag="IWL52_000592"
     CDS             49062..49808
                     /gene="truA"
                     /locus_tag="IWL52_000592"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9777178.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(50141..52810)
                     /locus_tag="IWL52_000593"
     CDS             complement(50141..52810)
                     /locus_tag="IWL52_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9777179.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(53048..53236)
                     /locus_tag="IWL52_000594"
     CDS             complement(53048..53236)
                     /locus_tag="IWL52_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9777180.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            53506..53868
                     /locus_tag="IWL52_000595"
     CDS             53506..53868
                     /locus_tag="IWL52_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9777181.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53920..55602
                     /locus_tag="IWL52_000596"
     CDS             53920..55602
                     /locus_tag="IWL52_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9777182.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            55719..57755
                     /gene="recG"
                     /locus_tag="IWL52_000597"
     CDS             55719..57755
                     /gene="recG"
                     /locus_tag="IWL52_000597"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9777183.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57804..58805
                     /gene="plsX"
                     /locus_tag="IWL52_000598"
     CDS             57804..58805
                     /gene="plsX"
                     /locus_tag="IWL52_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9777184.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58927..59169
                     /gene="acpP"
                     /locus_tag="IWL52_000599"
     CDS             58927..59169
                     /gene="acpP"
                     /locus_tag="IWL52_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9777185.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        18935 a        10724 c        12917 g        16638 t
ORIGIN      
        1 aatgtaaatg aatagttgta gtcagtcgat tgtgatacta gactcaaagt tgcaggaata
       61 agtttttcaa tttgtgattg gaagataaaa aatgaaaaag aagggctttt ttttatactt
      121 tgttgattgt agtgtttatt ttccctctgt ttagccaagt aacattttct atgactcaac
      181 cagtttcaag tgaggaacat gataaccttt ttcgtgctac agaagagttt aagaaatatc
      241 aggaaaatat tgttccggag actccattag ctagtgagcc aattactgat attgatggaa
      301 accaaatagg atggtatata cagtacgaag ttatggtggt aaataatgac tcaatagtaa
      361 aaaatacagt aggcttagag ctaacaccga ttgaaaaggt gagttcattg ttaacgtata
      421 tttatactga tgaaagtgac gagactgaga tcattttaac ggattactca aaaattgcta
      481 ctgataaaaa gtatatattg tggatgtaga cactagtgaa atactttatg atatagaaat
      541 tgatgaaaat ttactagatg tagcacaaga aatcgatcat caaaagcagg aagctattcg
      601 atatgctggt agaaaaggga aaagaaaata tttattacgt gaatgtagca aaagttagag
      661 aagatgaaaa tgagtggaaa gaattcaaga gtcgctactc aattaactcg actccgacat
      721 ttacagttta cagagaagga tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa
      781 tgtctttagc tgaagtagaa gagtttttgg attatgttag tatgcaacag taagggagtt
      841 cttattatga aaaattttga cacatattat gttattgttt tttcgatatc tttgctgtta
      901 atattactaa ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta
      961 tatttagtaa gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa
     1021 gtttgtttct taattagctt attgattcta taatattttt gatttatata ggaaattaaa
     1081 aatattatag aatcaacact cagagaacgg gttggttttt aatagattta ataagtactg
     1141 ataatgaaat ttctcctggt tgggaaaacg aagttggtta ccctgtagaa gaattttttc
     1201 aagattactt tgcggagtat gccgatagga taatgagaga ttacgtaaca gataaaactc
     1261 aagaagtcat caatcaatcc cagtcattaa atgaattatc tgataaggcg aaagaagaag
     1321 cagttgccat tatttcttca gggattagtg actatattga tatgagtggt gaaatcaatt
     1381 atggcgataa agacaaaatt gtacgcatga taaaagcttt tatcaaaact tttgatgtga
     1441 aggcagatct taagtttaat gatacttacc tagtaggaaa attaattaat atccttggga
     1501 cagagaaaga tacggaaacg tttatttcct ctttgtctat gttgtttaca gataagcatt
     1561 tttcaactca gcttggtgga gaggaactag tggaaatctt ttaaccatta cgcccagcat
     1621 taataaaagt atatcaaaaa agtactcctg atgattttta agattggttt gagaaataat
     1681 gtgaaaacac tatttttaat gttaattata gatagagatg aacgcgtcat atggttaaaa
     1741 gaatgaaaaa gtcaaagata cactgctata tttcactaag gatatagcag tgtatctttc
     1801 atgtcgtttc actggcagtt ttttataaaa ggaggtgtgc atttcagttg cttttctttg
     1861 gtagttagtt gaatcgttta agaattaacg gatgtggcag acaggtgctc tgtccaaaaa
     1921 ccagtaaaat ctttgatggt tccatcttta tttagctgtg cctgttcaaa tactgttttg
     1981 tttttaccga atggatcaat tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt
     2041 aatcctgagt aagcatcttc gataacaaga caatcagccg gagctaagct cagtttgtct
     2101 gccgctttaa gaaaaatatc cggttgcggt ttcccaggga aagagccgtc gtcatagaca
     2161 atcttgtccc agtcaaacca gcgggctaaa tcgaaaatat caaaataaaa agcaacattc
     2221 tctttgacag tagccgtggc aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga
     2281 gtcgcttcaa gcccttttgt caactgaagc tcgttttcat tttgcaagca aagcatacga
     2341 taatagcttt ctttttcttc tgacaatacg gcgatctcag aggcggtcaa atgctctgaa
     2401 atgaaatgtt tcaaaatctc cgtattcgtc cgaccatgaa tattttttaa aatatcctcg
     2461 tctgataaac catcttttgt atgatcatgt atcatatgca gccaagcagc ttcatgcaga
     2521 tgagagtcca aaaacattgt tccgttgaaa tcaaatataa tccctttcat agttacgctc
     2581 ctatagtggt attttcggtt gaatcagcaa tttgcagatc gacatattgt gcatagtgct
     2641 cttgaacctc ccgcgagatc tggcgatccg taatgagcag atcgaaatca tgcaaattat
     2701 aaaacacata aaaatcatct cgattgaatt ttttattatc aacgagtaga tatttcttac
     2761 gggaattatt tagagcgatc tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg
     2821 cttcgttgtg aacgccattt gaactgataa atgcttttgt aaactttagt cttcttaaat
     2881 tgtcgttggt gatcggtccg acaaatgcgc cggtattttt ccggaaatca ccgccaatca
     2941 gtagaatttc tggtccatct gcttgagcaa gaatatcaaa aactggataa ttatttgtaa
     3001 tgatccgaat tttcttgttc agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc
     3061 ctaagaaaat cgtttctccc tcgtgaatca aggaggctgc tgtggcagca atttcttttt
     3121 tttcttgaat ctgcagagtg gctttttcag tgtgagaaag ttcttgatct aaagtgtagg
     3181 aaagactttg agcccctcca tggacacgca gcagcttgcc cgatttttct aattcatcta
     3241 aatctctgcg aacggtcata tcagaaacat ctaattgatt gatgatttca ttaacggtaa
     3301 ggattccttt tttattgacc atttctacga tagtaagcaa tctttctttt ttcaacattg
     3361 tcttcacctc taatttaagg ataatctatc atataaacgg acaacagtca atctaattcg
     3421 aacaaaagta aacatttagg gtggttttta tgtttgttac aatagatttt attgctgttt
     3481 tctggttttt atgattattt gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca
     3541 atgcaatcag ttagaaagag cgaaaactga ttatagattt gaggagagat gtacatgctt
     3601 aaattaccag aagattttat ttttggcgga gccacagcag cctatcaggt tgaaggagcc
     3661 actaaggaag gtggcaaagg cgctgttgct tgggatgact ttttagagga acaagggcga
     3721 ttcagtcccg atccggcaag tgatttttat catcaatatg caaaggacat tgaattgtgt
     3781 gaacgttttg gtgtaaatgg tctaagatta tccattgctt ggagccgcat ttttcctgat
     3841 ggagctggga aaccaaatcc agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt
     3901 aagaaaagaa acgtaacgcc gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg
     3961 tttgaccatg gagatttttt gaatcgagaa acaatagaag catttgtttc ctatgccatt
     4021 ttttgttttc atgaattcaa agaagtcaag gtgtggagta cctttaatga aatctatcca
     4081 gttgcaacca atcaatattt gttaggtgtt ttcccaccag gaatcaaata tgattttaca
     4141 aagatcgtcg cctgtcttca taatatgatg gttgctcatg cccgtgtagt gaactatttc
     4201 aaagaaaacg aacttccggg tgaaattggt gtagttcact cattagagac gaaatatgcg
     4261 gcaacagacg cgccggaaga taagcatgct gcctttttag acgatgcatt gtcgatccgt
     4321 ttcttattag atgctaccta tttaggctat tattcaactg aaaccttgac tgctcttgat
     4381 gagatatgtg aagcaaatca ggccagctat cattttcctg aagaagattt cgttgaattg
     4441 aaaaaagcat ccacgcgtaa tgactactta gggatcaatc actaccaatg ccattttgtc
     4501 aaagcttacg atggagaaaa tgcaatccat cataacggta cgggtgaaaa agggacctct
     4561 gtttataaag tgaagggaat cggagaacgt atttataaag aaggaattcc tcgaactgat
     4621 tgggactggc tgatttatcc agaaggtcta tatgacttgt tgctgcgaat taaaagcgat
     4681 tacccacatt acaacaaaat ctatatcact gagaacggga tggggtataa agatcagttt
     4741 gaagatggca ttattatgga tcagccgaga atcgactatt tgagagtcta tttggaatcc
     4801 ttaagcaagg cgataacagc cggggtaaat gtgaaggggt attttttgtg gtcgcttatg
     4861 gatcttttct catggacaaa tgggtataac aaaaggtatg gcttgtttta tgttgatttt
     4921 gaaacccaga agcgttaccc aaaagaatca gcttactggt acaagttggt cagtgaaacc
     4981 aaaacgatta tttaataagt gataatggag gaagaaaaga tgaataaaga ggaattacaa
     5041 atgcttggtt ttgagatcgt tgcatattca ggcgacgctc gcagtacatt attgaaatta
     5101 ttgaaagaag taagacaagg aaactttgaa catgttgatt cggcgttgaa agaagccgac
     5161 gaaaatttaa cccttgcaca taattcgcaa acaaaaattt tagcggaaga agcatctggg
     5221 aaagagatgg agatgggatt catttttatc catgggcagg atcatttaat gaccacttta
     5281 ttgctgagag atctgattca agactttatt gtactgtaca gacagaatca aggataggag
     5341 aagaacgcta tggatggaat cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa
     5401 atctcacgta atagttattt aggagccgtt cgtgacggat ttttaacagc gatgccagct
     5461 attttatttt caagcatttt cattttgatt gctgcggttc ctgagatttt tggttaccaa
     5521 tggcctgaat ctgtctccac atggctatgg aaagtctata attattcgat gggtgttgta
     5581 gggttactcg tttcagcaac cactgctcgc tgcttggctg attcgatgaa tcgtcgctta
     5641 ccgaaaaaca aagtaatcaa caccacctct gtgatgctgg cctctgtggt aagtttcctg
     5701 ttgttaagcg tctctccgat tgaaggtgga ttttcaacgg aatatatggg gacaaaggga
     5761 ctactggctt cctttgtcgc tgcattttta acagttaatt tatataagtt ttgtgtgctg
     5821 aaagatatta ccattaaaat gcctaaagaa gttcctggaa cgatttcgca aaccttccga
     5881 gatatttttc ctttctcttt ttcagtattt gcggccgttt taattgattt aggcattcga
     5941 caaatcttcc aaatctcatt tgcagaagca atcattacgg tgctgcagcc gctatttaca
     6001 gcagctgacg ggtacttagg aatcgctatt atctggggag cgatggcgct gttttggttt
     6061 gtcggtgttc atggaccgtc gatcgtggag ccggcaattg cggcaattat ctatgccaat
     6121 gtggaaacca atctgcaatt gttcaattcc ggtgaacaag cttctaaagt actgacggtg
     6181 ggtttaggga attttgtagg aactatggga ggaactgggg caacattggt tgttcctttt
     6241 atcttcctac tctttgctaa gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt
     6301 ccagtaagtt tcgctgtcaa cgaaccattg ctatttgcgg cacccatgat tttgaaccct
     6361 tattttttta ttccattttt gttaacacct gttgtaaacg tttgcctgtt caagtttttt
     6421 gtcgatgtat tgggcatgaa cagctttatg tacgttttgc cttgggcaac tccagcaccg
     6481 atcggtttgg ttctaggaac gggcatgggt atcttagctt ttgttctagc agcggttcta
     6541 atcgtcgttg acttcctgat ctatctacca ttttgcaaag catacgatgc tgtcttagta
     6601 gagaaagaag cacaaaaagc agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta
     6661 cgtgaggaag tacctgtggc agcgagtcaa gttatcagcg atgcaaataa agaaatcaat
     6721 gtgttggtac tttgtgctgg cgcagggaca agtgcgatgt tagctaatgc tttaaaaaaa
     6781 ggtgcatctg aatcgaaagc accgatcagt gcaatggcgg gtgcttatgg ttctcattac
     6841 gaaatgctgc aggattttga tatggtggtc ttagcaccgc aagtcaattc ttactatgat
     6901 gatatcaaag aagacaccga tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa
     6961 tatatcggat taacgagaga tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa
     7021 taaaggagga gccataatga aaatgggaat ttggttgcgt aaagctgttt tatttgcagc
     7081 tgtgttaggg gctttggggg ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat
     7141 tcgcggagca gatatttcga tcctgtcaga tatggaaaaa tctggtgcaa aatattatga
     7201 agacgatatt tcaaaagatg ccttagagat tttaaaaaat aatggtgtca actatgttcg
     7261 actgcgtttg tggcaggatc cttataatag taatggagaa agttatggag cagggacaaa
     7321 tgatctggaa acgacgattg accttgcaaa acgagcaaaa aatctgggac ttaaggtgct
     7381 gctggatttt cactacagtg atttttgggt tgatcctggg aaacagaatc ttcctaaagc
     7441 atggcaaggt ctgacctttg aagaaatgaa tacggcattg tacgactata caaagaacgt
     7501 tctgtcggaa atgaagcagc tggacgttta tcctgatatg gttcagatcg gcaatgaact
     7561 gaatagcgga atgctctggc cttatggcaa gtcttgggga gaaggcgggg gagaatttga
     7621 tcgattggct gcttttttga agtctggtat ccaagctgtt cgagacactc aacctaaaac
     7681 aactccagta atgctccact tagctgatgg tggcgataca ggagcattta cttggtggtt
     7741 tgatgaaatc acgagtcgag gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta
     7801 ttggcacggt tcaatgaatg aacttcaaac caatatgaat gcgatcagcc aacggtatgg
     7861 taaagaagtg atcgtagttg aaacagctta tgggcataca acagcgaatg ccgatactat
     7921 gccaaatgct tttggtgaag ccgaagcagc tgcgggggga tatgaaccaa caccgacagg
     7981 acaagcagag tatttgctgg atcttgccga tcggattcag gcagtaccga ataatcgcgg
     8041 ggcgggcttc ttctattggg aacctctttg gtacaacgga aatgtgagtt gggcaacaca
     8101 agcaggtatg aattatctca gtgttcaatc cactatggga aacgaatggg acaaccaagc
     8161 gatgtttgat tttgctggaa acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc
     8221 gcaaataaat ctagttaaaa atgcaagttt cgaaagcgat ggaccaacga ccagtccttc
     8281 caattggcaa acctggtttc aacaaggaac cgctaagggt acgatcaaaa cggaagcggg
     8341 cacttttttt ggggactaca aactaacttt ttggcaggat caagcatacg aggcatcagc
     8401 ttatcaaaat gttagtggca taagcaaagg aacttacaca ttgagtgcct atgtaatgtc
     8461 tgggggagag tttgatacta atcagttata tatcaaaaat tatggtggag cggaactaaa
     8521 tactgcggtt gttaaaacaa gtgaatggac aaaaattaca atcaaagaca ttccaattac
     8581 aacaggtgtt tgtgaaattg gggtttatag caaggcttca ggaaatgctt ggacgaacgt
     8641 tgattttgtt tcattggtga aacaatagac tgcggatcgt ttcgattcaa gatcggaggt
     8701 tcccttcagc cacatcgtta gcgtagaagg caggaatcga acctgcacct cgcctttgcc
     8761 aaaaataacc agaaacgatt tccagtcatt tttaccaaaa cgagtaaagc accagacttc
     8821 atctacactt tgtattatgc taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa
     8881 ttgcaaaaag ttgtgcagat aacataaagt ttgtttaaga aagggtaacc ttagaattcc
     8941 gtcgtgaata tgatttgagc acttagatgg atagaaaaaa gttctcggaa aacagttcgt
     9001 ttttcgagaa cttcttttta tgataagaat gaaacaaatt aacttatatt atagtctctt
     9061 ttggtaggga accatcttat ttttaatgaa aatcaagcat tttagctatt gaagaatgtt
     9121 gatttatagg gattccccct ccatattatg ttggctcttt attcccactc cactgttgct
     9181 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
     9241 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
     9301 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
     9361 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
     9421 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
     9481 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
     9541 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
     9601 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
     9661 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
     9721 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
     9781 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
     9841 ttcaacttgt ctaagaagcg atctcttgca ttgactttga tgatgttcaa gccgaatttt
     9901 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
     9961 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
    10021 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
    10081 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
    10141 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
    10201 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
    10261 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
    10321 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
    10381 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
    10441 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
    10501 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
    10561 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
    10621 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
    10681 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
    10741 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
    10801 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
    10861 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    10921 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    10981 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    11041 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    11101 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    11161 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    11221 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    11281 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    11341 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    11401 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    11461 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    11521 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    11581 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    11641 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    11701 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    11761 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    11821 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    11881 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    11941 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    12001 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    12061 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    12121 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    12181 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    12241 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    12301 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    12361 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    12421 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    12481 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    12541 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    12601 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    12661 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    12721 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    12781 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    12841 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    12901 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    12961 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    13021 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    13081 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    13141 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    13201 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    13261 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    13321 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    13381 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    13441 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    13501 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    13561 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    13621 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    13681 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    13741 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    13801 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    13861 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    13921 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    13981 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    14041 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    14101 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    14161 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    14221 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    14281 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    14341 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    14401 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    14461 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    14521 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    14581 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    14641 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    14701 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    14761 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    14821 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    14881 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    14941 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    15001 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    15061 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    15121 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    15181 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    15241 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    15301 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    15361 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    15421 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    15481 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    15541 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    15601 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    15661 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    15721 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    15781 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    15841 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    15901 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    15961 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    16021 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    16081 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    16141 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    16201 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    16261 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    16321 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    16381 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    16441 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    16501 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    16561 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    16621 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    16681 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    16741 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    16801 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    16861 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    16921 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    16981 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    17041 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    17101 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    17161 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    17221 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    17281 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    17341 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    17401 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    17461 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    17521 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    17581 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    17641 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    17701 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    17761 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    17821 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    17881 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    17941 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    18001 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    18061 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    18121 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    18181 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    18241 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    18301 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    18361 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    18421 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    18481 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    18541 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    18601 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    18661 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    18721 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    18781 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    18841 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    18901 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    18961 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    19021 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    19081 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    19141 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    19201 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    19261 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    19321 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    19381 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    19441 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    19501 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    19561 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    19621 ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    19681 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    19741 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    19801 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    19861 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    19921 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    19981 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    20041 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    20101 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    20161 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    20221 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    20281 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    20341 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    20401 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    20461 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    20521 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    20581 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    20641 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    20701 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    20761 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    20821 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    20881 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    20941 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    21001 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    21061 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    21121 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    21181 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    21241 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    21301 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    21361 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    21421 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    21481 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    21541 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    21601 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    21661 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    21721 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    21781 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    21841 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    21901 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    21961 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    22021 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    22081 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    22141 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    22201 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    22261 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    22321 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    22381 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    22441 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    22501 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    22561 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    22621 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    22681 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    22741 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    22801 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    22861 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    22921 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    22981 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    23041 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    23101 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    23161 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    23221 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    23281 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    23341 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    23401 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    23461 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    23521 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    23581 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    23641 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    23701 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    23761 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    23821 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    23881 aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    23941 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    24001 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    24061 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    24121 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    24181 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    24241 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    24301 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    24361 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    24421 catgtttgat gaatattgtg tcttgtgacc gagaagttgg caggagaaaa ccgtttatac
    24481 gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt
    24541 agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg
    24601 gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc
    24661 gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa
    24721 tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag
    24781 gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag
    24841 tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt
    24901 cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt
    24961 cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca
    25021 agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc
    25081 cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat
    25141 ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag
    25201 aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt
    25261 ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag
    25321 attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg
    25381 tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat
    25441 aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt
    25501 cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga
    25561 tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc
    25621 actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat
    25681 cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt
    25741 ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc
    25801 cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca
    25861 cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag
    25921 ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc
    25981 ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca
    26041 aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt
    26101 ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta
    26161 ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat
    26221 aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac
    26281 caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt
    26341 tttcttcaaa aatatgaagt agctgttggg aaccataagg gatcacttca actgggagtg
    26401 ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg
    26461 agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa
    26521 aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa
    26581 tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa
    26641 ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat
    26701 aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt
    26761 caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc
    26821 gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc
    26881 aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat
    26941 tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa
    27001 gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct
    27061 ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa
    27121 ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa
    27181 caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg
    27241 ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata
    27301 tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca
    27361 taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg
    27421 ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca
    27481 aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg
    27541 aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta
    27601 cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac
    27661 tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca
    27721 gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta
    27781 gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt
    27841 gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta
    27901 ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc
    27961 ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa
    28021 gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct
    28081 ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag
    28141 atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt
    28201 aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg
    28261 tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac
    28321 tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa
    28381 tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa
    28441 gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt
    28501 gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc
    28561 gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta
    28621 catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac
    28681 ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga
    28741 acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc
    28801 gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa
    28861 ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta
    28921 gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca attactattg
    28981 gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac
    29041 cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta
    29101 gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct
    29161 ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt
    29221 ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga
    29281 gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa
    29341 aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt
    29401 gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa
    29461 gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt
    29521 atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa
    29581 gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc
    29641 gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa
    29701 tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt
    29761 gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt
    29821 gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat
    29881 gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca
    29941 gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct
    30001 gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt
    30061 atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg
    30121 tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca
    30181 tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc
    30241 ggaaaagctg aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac
    30301 tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc
    30361 ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat
    30421 tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc
    30481 ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag
    30541 aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa
    30601 cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct
    30661 cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa
    30721 atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa
    30781 cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta
    30841 gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta
    30901 acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct
    30961 ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa
    31021 tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg
    31081 ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc
    31141 gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact
    31201 ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct
    31261 ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt
    31321 acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt
    31381 ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
    31441 gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta
    31501 acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc
    31561 cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact
    31621 taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtctcagg
    31681 atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata
    31741 atagaaggaa cgcgaaataa aagagtcagt attttctaaa tgaaacttta tgaaaacaaa
    31801 atgtttttta caagaaaaag ccttgctttg tgagtttata tatattataa tgacaaaggt
    31861 gcgtttactt tagtgaattg cacagacgta atcagaatga tttcgtcggc taagaaacga
    31921 gaggttgcga cacgcccgga cgctttgcca cgaacgagcg tgacggaaat tttcgtggag
    31981 ctatgtctac ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca aaaaattcgt
    32041 atccgtttaa aagcgtatga acaccgtatt ttagatcaat cagcggataa aatcgtggaa
    32101 actgcaaaaa gaactggagc tagcgtgtct ggtccaattc cattaccaac tgaacgcagt
    32161 ctttacacaa tcatccgtgc gactcataaa tacaaagatt cacgcgaaca attcgaaatg
    32221 cgtacacaca aacgtcttat cgacattgtg aacccaacac caaaaactgt tgatgctttg
    32281 atgaagctag acttaccatc tggtgtaaac atcgaaatca aattataaaa ctaaaacaaa
    32341 aataatggag gtgtactcat gaccaaagga atcttaggga aaaaagtggg aatgacacaa
    32401 atcttcactg aatctggtga attaattcca gtaactgttg tagaagctac tccaaacgta
    32461 gtactacaag tgaaaacaat ggaaacggat ggctacgaag ctattcaagt tggttaccaa
    32521 gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa agcaaacacg
    32581 gctcctaagc gcttcattcg tgaattcaaa aatgttgagc taggagaata tgaagtagga
    32641 aaagaaatta cagtagatgt tttccaagca ggagacatta ttgatgtaac aggtactacg
    32701 aaaggtaaag gattccaagg ggttatcaaa cgccacggac aaagccgcgg accaatggct
    32761 cacggttctc gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc taaccgtgta
    32821 tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat ccaaaacttg
    32881 gaagttgtac gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa tgttcctgga
    32941 gctaaaaaat ctttaatcac tatcaaatca gctgtgaaag ctaaataatt gtaggaagaa
    33001 aggaggaact aaggaatgcc gaatgtagca ttattcaaac aagatggaag ccaaaacggt
    33061 gaaatcacac ttaacgaaga aatcttcgga attgaaccta atgaaagtgt tgtttacgat
    33121 gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac acgcggttaa aaaccgtagc
    33181 gctgttcgtg gcggtggacg taaaccatgg cgtcaaaaag gaacaggtcg tgcacgtcaa
    33241 ggatctatcc gctcaccaca atggcgtgga ggtggcgtag tctttggacc aacaccacgt
    33301 tcttacagct acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc tgtattgtca
    33361 gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct tgaacttcga cgcaccaaaa
    33421 acaaaagaat tcaaacaagt tcttgctaac ttatctatcg actctaaagt attggttgtt
    33481 ctagaaaaag gtaacgactt tgctgcttta tcagcacgta acttaccaaa cgtttctgta
    33541 gtaacttctg ataatgtgag tgtgctagac gttgtatcaa atacaaaagt gttagcaaca
    33601 caaactgctc ttactcaaat tgaggaggtg cttgcataat gaacttacta gacgtaatca
    33661 aacgcccagt gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa tacacttttg
    33721 aagtggacac tcgcgcaaac aaaactttag taaaacaagc ggtagaagct gcttttgacg
    33781 ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt atgggcaaat
    33841 atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa gattcaaaag
    33901 aaatccaatt attcgaagct gctgaataag cactaataga taactcatta aattaggagg
    33961 gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta acatgacagg
    34021 ttctgatttt gctgaaatca caacatcaac acctgaaaaa tcattgttac aaccattgaa
    34081 aaaccatgct ggacgtaaca acaacggccg catcactgta cgtcatcaag gtggcggaca
    34141 caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg ttgcaactgt
    34201 tcaaacgatc gaatatgatc caaaccgttc agctaacatc gcgttagtac actatgaaga
    34261 tggagtgaaa gcttacatct tagcacctaa aggactagaa gtgggtatga aacttgtttc
    34321 aggtccagaa gcagatatca aagtcggaaa tgctcttcca ttagaaaaca ttccagttgg
    34381 tactgttatc cacaacattg aaatgaaacc tggtaaaggt ggacaattga tccgttctgc
    34441 tggtacaagt gctcaagtac ttggtaaaga aggcaaatac gtattgatcc gcttgaactc
    34501 tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca atcggttctg taggtaacga
    34561 acaacacgaa ttaatcaaca tcggtaaagc tggacgctct cgttggatgc gtaaacgccc
    34621 aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg gtgaaggtaa
    34681 agctcctatc ggacgtaaag caccagtttc tccttggggt caaccagctc ttggttacaa
    34741 aactcgtaac aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca aaaataaata
    34801 attactttga ccatgtgtcg cacattttga gaggaggttc accatgggtc gtagtttaaa
    34861 gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag caggggttga
    34921 aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa gttttgttgg
    34981 atataccatc gcagtttatg atggacgtaa acatgtacca gtttacatcc aagaagacat
    35041 ggtaggacac aaattaggtg aatttgcacc tacaagaact tatcgtggac atgctgctga
    35101 cgataagaaa acgaaacgct aatttgaggg gaggataaat ccaaatggca gaacaaatca
    35161 catcagctaa agcaactgca aaaacagttc gcacttcacc tcgaaaagcc cgtttagtaa
    35221 ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa ttcacaccaa
    35281 acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat gcagaaaata
    35341 actttgactt agatgttgaa aacttggtag taagcgaagc ttttgttaac gaaggaccta
    35401 caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac aaacgtacaa
    35461 gtcatatcac ggtagtcgta tcagaaaaat aaggagggat aatcagtggg tcaaaaagta
    35521 catccaattg gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg gtatgctgaa
    35581 aaagagtatg ctgagttctt acacgaagat ttaagaatcc gtaaatttat ttcaactaaa
    35641 cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg cgtaaacatt
    35701 tcaattcaca cagctaaacc aggtatggtt attggtaaag gcggttctga agtcgaaagc
    35761 ctaagaaaag aattaaacaa attaactggt aaaagaatcc atatcaatat cgtggaaatc
    35821 aaaaaaccag atctagatgc aaaattagta ggtgaaggaa tcgcacgtca attagaaaac
    35881 cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg tgctggcgct
    35941 caagggatca aaactcaagt atctggtcgt ctaaacggag cagatatcgc tcgttcagaa
    36001 ggatactcag aaggaactgt tcctcttcat actttacgtg cggacattga ttacgcatgg
    36061 gaagaagcag atacaacata cggaaaacta ggagttaaag tgtggatcta tcgtggagaa
    36121 attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta gtacctaaac
    36181 gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa ggcggaaaag
    36241 aagtagcatt cggtgaatac ggtttgcaag ctgttgattc acattggatc acaaaccgcc
    36301 aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa acgtggtggg aaagtatgga
    36361 ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat tggggtacgt atgggttctg
    36421 gtaaaggggc acctgaagga tgggttgcac cagtaaaacg tggtaaaatc atgtttgaaa
    36481 tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg tctagcttct cacaaattac
    36541 caatgaaaac taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat gaaggttaaa
    36601 gaaatcagag aattaaccac tgccgaaatg cttgatcaag aaaaacaatt aaaagaagaa
    36661 ttgtttaatc ttagattcca attagcaaca ggtcaattag aaaacactgc acgtattaaa
    36721 gaagtacgta aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc taagtaatta
    36781 tggaaggagg ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt ttatcaaggt
    36841 cgcgtggttt cagacaaaat ggataaaaca attaccgttg ctgtcgaaac aaagaaaaac
    36901 caccctatct atggcaaacg catgaactat tctaagaaat acaaagtgca tgatgaaaac
    36961 aacacagcaa aagttggcga catcgtgaga attatggaaa ctcgtccatt atcagctaca
    37021 aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta ttatctaata acacgagatt
    37081 ttcctatata gattgaatgc aaaatctgaa aggaggatac acaacgtgat ccaagcagaa
    37141 agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa ttttgacgat caaagtcctt
    37201 ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac ggttaaacaa
    37261 gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta aagccgttat cgttcgaaca
    37321 aaatcaggag ctcgtcgtac agacggttca tatattaaat ttgatgaaaa tgctgcagtg
    37381 attattcgtg atgataaaag ccctcgtgga acacgtatct ttgggccagt tgcacgtgaa
    37441 ctacgtgaaa acaacttcat gaagatcgtt tcactagcac cagaagtttt ataatcttga
    37501 gacacttatc aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga taaagttaaa
    37561 gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt cccgaaaaaa
    37621 gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc ttctcaagct
    37681 gctcctcaag gtggaattgt tgagatggaa gcgccaattc atgtttctaa cgtgatggtg
    37741 gttgactcta ctggtgtagc tggccgagtt ggctacaaag aagtagacgg caaaaaagtc
    37801 cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa agtttaagga aggagggtct
    37861 tactgaatga accgcctaaa agaaaaatat cttaaagaag taactccatc attgatggaa
    37921 aaatttaact atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt catcaacatg
    37981 ggtgttggtg atgcggtatc aaacgctaaa aacttagata aagcagttga agaattaact
    38041 ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc tggattccgt
    38101 ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc gcggagaaag aatgtacgaa
    38161 ttcttagaca aattggtttc tgtttcacta ccacgtgtac gtgacttcca tggtgtaagt
    38221 aaaaaagcct ttgacggtcg tggaaactat actttaggta ttaaagaaca attgatcttc
    38281 ccagaagtag attatgattt agtagataaa gtacgtggta tggacatcgt tattgtaaca
    38341 acagcaaact ctgatgaaga atctcgtgag ttgttgacac aattaggcat gccattccaa
    38401 aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa aaacaaacgt
    38461 cctgcaaaat tttcaacaca agcttatact cgttgcgaac gttgcggacg tccacattca
    38521 gtttatcgta agttccatct ttgccgtatt tgcttccgcg aacttgccta taaaggtcaa
    38581 attcccggcg tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa ggaggtaaaa
    38641 gatcaatggt catgacagat ccaattgcag attttctaac tcgtatccgt aacgcaaaca
    38701 tggttaaaca tgagtcttta gaagtacctg catcaaaaat taaacgtgac atcgctgaaa
    38761 tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac aaacaaggcg
    38821 tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca aacttaaaac
    38881 gtatctctaa accaggtttg cgtgcttatg tcaaagctga cgaagtacca aaagtattga
    38941 acggtttagg tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat aaagaagctc
    39001 gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg gtaatcaagt agaatctaca
    39061 aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg ttgttatccc tgaaggagta
    39121 acagttactc aagacggaaa caacgttaca gttaaaggac caaaaggtga attgactcgt
    39181 actttttcag cagatatcaa aatgaatatc gaaggaaacg aagtaacttt cactcgtcca
    39241 aacgacagta aagaaatgaa aacaattcat ggaacaactc gtgcgaactt caacaacatg
    39301 gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac taatcggggt tgggtaccgt
    39361 gcacaattac aaggatcaaa acttgtattg aacgttggtt actcacatcc agtagaaatc
    39421 acaccaccag ctggagtaac tgttgaagta ccagctaaca cacaagtaat tgttaaaggt
    39481 gcgaacaaag aagaagttgg cgaattagct gcaaacattc gtggaactcg tcctccagaa
    39541 ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa agaaggtaaa
    39601 actggtaaat aatcgtttag aaaaattgca tagaagccag cagcaggaaa aaggcatttg
    39661 gctgaagttt accttcaaaa aaatacattt ttacttgtgc cgcagcttct atttttcgaa
    39721 acgtatttac tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa ttgtgattac
    39781 aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta acaatatctc
    39841 tggtactgct gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca tctacgctca
    39901 aattattgat gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg ataaagaaat
    39961 ttcaggtgga acaaaaacag aagcagcaca agctgtcggt aaactagttg ctgaacgtgc
    40021 agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt ggataccttt accatggccg
    40081 tgtgcaagct ttagctgaag ctgctcgcga aaatggacta gaattttaga agaaggagga
    40141 ataccattca tggtttatat cgatccaaaa catttggaat tagaagaccg cgttgttgca
    40201 attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc tgctttagtt
    40261 gttgtcggtg acaaaaacgg acacgtaggg tttggtacag gtaaagctca agaagttcct
    40321 gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt acctatggtt
    40381 ggttcaacaa tcccacacga agtaatcggt gcttttagtg gtggtagaat cctaatgaaa
    40441 cctgctgtag aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt attagaatta
    40501 gctggggtag cagatatcac ttctaaatct ttaggctcaa atacaccaat caacgttgtt
    40561 cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc agaacttcgc
    40621 ggcaaatcag ttgaagaatt aatcggataa ggaggacaac aataatggct gaattaaaag
    40681 ttactttaaa acgcagtatt atcggacgtc ctcaaaacca acgcgataca gttaaagcgt
    40741 taggtcttac aaagattaac agttcagtgg ttaagccttc taatgaagca attaaaggca
    40801 tgatcaatac tgtttctcac ctagtggacg ttgaagaaat ttaatttaga aaaatcataa
    40861 ctagaacatt aaggaggtgc caaacctatg aaacttcatg aattaaaacc tgctgaaggt
    40921 tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg aaaaactgcc
    40981 ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt aggatttgaa
    41041 gggggacaaa caccattgtt ccgtcgttta ccaaaacgag gattcacaaa cgtgaaccgt
    41101 aaagactatg cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg agctgaagta
    41161 actcctgttg ctttagttga agctggaatc gtgaaaaacg aaaaagctgg aatcaaagta
    41221 ttagctaatg gggaactaaa caagaaatta actgtgaaag cagcaaaatt ctctaaagca
    41281 gctcaagaag caattgaagc ggctggtgga tcaatcgagg tgatctaatg tttagactat
    41341 taaaagatgc ttttaaagta aaagacatta gatcgaaaat cctatttact gtatttgtgt
    41401 tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct gcttcattgc
    41461 aaaacctttc ttctttgcca tttttcaaca tgttgaactt ggtaagtggt agtgcgatgc
    41521 aaaacttttc cattttctca atgggggttt caccatacat tactgcttct atcgtcattc
    41581 agcttcttca aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa ggggaagttg
    41641 gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt agtattggca tttatccaat
    41701 ctgtcggtat cactgctgga ttcaatcaat acgctcaatt aggtttcgtg aataatccta
    41761 acgtagcaac ttacgtaacg atcggtttga ttctaacagc cggtacgatg tttgttactt
    41821 ggctaggaga acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg
    41881 caggtatcat ttctagattg cctgaaagta tcagagaaat ttatgaagat tactttataa
    41941 atattgatcc ttcagacata tggaaatctg ctatttttgt ggccatttta ctagtagcgg
    42001 tcttgttgat cgtgacattc gtaacatttt tccaacaagc tgaacgcaag atcccgatcc
    42061 aatatacgaa acgcgtagct ggcgcgccta caagcagcta tctgccgttg aaagtaaatg
    42121 cagctggagt tattccagtt atctttgcta gttcgtttat tgctacgcca aatgctgttt
    42181 tacaagcatt gagcggtaga ttcggttctg aaaactggta caaagtgatg atggagatct
    42241 ttaactataa tacagcacct ggtgctacga tttatacgat tctgatcgtt gccttcactt
    42301 tcttctacgc ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg caaaagcaag
    42361 gaagttatat accaagcgta cgccctggta aggggactga agagtatgtc tcaagattac
    42421 tgatgcgttt aagtacagtt ggtgcagtct tccttggttt agtggcattg cttccaatca
    42481 ttgctcaaat ggtttgggat cttccacaat caatcggatt aggcggtaca agcttactga
    42541 tcgttatcgg tgtagcttta gaaactgcga aacagttaga aggcttaatg ctgaaacgta
    42601 aatatgttgg atttattgag taagaaagcg tgttgaggtt tcccctcaac acacgttctc
    42661 acacatttat taggagggaa aacaatgaac ctcattttaa tggggctgcc aggcgcaggt
    42721 aaaggaacac aagctgaaaa aatcattgat gcttatggta ttccgcacat ctcaacagga
    42781 gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc aaagtcttat
    42841 atggataaag gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta
    42901 gctgaaccag atacagagaa aggcttctta ttagatggct ttccacgtac gctagatcaa
    42961 gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat
    43021 atccatgttg gtgaagaaat cttggtcgaa cgtctagccg gccgatttat ctgcagcaat
    43081 tgtggagcaa cttaccataa aattttcaac cctacaaaag ttgaagacac atgtgatcgt
    43141 tgcggtgggc atgaattcta tcaaagagaa gatgataaac ctgagacggt taaaaatcgt
    43201 ctggctatca acatcaaaaa tagtgaacca atcttggctt attataaaga acaaggtttg
    43261 ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc
    43321 attgaaaaat agcacgattg cccgtagccg acttgcatta tgcagttact cttgtcaagt
    43381 tcaataaatt atgatagaat atgtcagtcc gacttctaga gaatcctcta gatgatccat
    43441 tttgagaatt ttgaggtggg aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa
    43501 caagtacaag gaggtactgt gcgtggcaaa agaagatatg attgaagtcg aaggtacagt
    43561 cgtcgaaact ttgccgaatg caatgtttaa agttgaacta gaaaacggac accaagttct
    43621 tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg gtgacaaagt
    43681 aacggttgaa ttgtctccat acgatcttaa ccgtggccgt attacttatc gctttaaata
    43741 attgtactcc gtaaaactac aggaggtatt atcatgaaag taagaccatc agtaaaacca
    43801 atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca
    43861 aatccaaaac ataaacaacg tcaaggataa ttggaggtgc tataaatatg gctcgtattg
    43921 caggagtaga tattcctcgt gataaacgtg tagtagtttc acttacttat atctatggta
    43981 ttggtaacac tactgctaaa aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg
    44041 ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc agaagtagat aaattaaaag
    44101 ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt
    44161 cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca
    44221 atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa
    44281 gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga
    44341 agaatataga agcaggtatt gcacatatcc attctacatt caataacact atcatcatga
    44401 tcactgatac tcacggaaat gcattagcgt ggtcatcagc tggttcatta ggtttcaaag
    44461 gaagcaaaaa atcaactcct tttgccgctc aaatggcagc agaaactgct acaaaagcag
    44521 caatggaaca cggattaaag actgttgaag taacagttaa aggacctggt tctggacgtg
    44581 aagcagcaat tcgttcatta caagctacag gtttagaagt gactgcaatt cgtgacgtga
    44641 ctccagttcc tcataatggt gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc
    44701 tcattttgag ttttatcagt aacgtcattg aacaagacac ttttcgtttt gaaaggggta
    44761 aagataagaa tgattgagtt cgaaaaacca agaatcgcaa aaattgatga agaaaaagat
    44821 tatggcaagt tcatcgttga acctcttgaa agagggtatg ggactacttt agggaattcc
    44881 ctacgtcgta ttttattatc ttctttgcca ggtgctgcca tcactaatat tcaaatcgat
    44941 ggtgtactgc atgaattctc taccatcaaa ggtgttagag aagacgtcac tcaaatcatc
    45001 ttgaacatta aaggcttagc gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc
    45061 gatattaccg gacctgctac agtaactgct ggcgatatta tcgttgatag tgatgtagaa
    45121 attcttaaca aagatatgta catctgtagt gtctctgaag gagctacgtt ccatgctcgc
    45181 ttaacagtga aacctggacg tggttatgtt caagcagatg aaaataaaaa agaagatatg
    45241 ccaattggcg ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa
    45301 gttgagaaca cacgtgttgg acaccgtgaa gattttgata aattgacgat ggagatttgg
    45361 acagatggtt caattgaacc gttggaagca atgagtttgt ctgcgaaaat catgactgaa
    45421 catttggata tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat catggtcgaa
    45481 aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt agacttgtct
    45541 gttcgctctt acaattgttt gaaacgtgca ggaatcaata ctgtacaaga acttacaaat
    45601 aaatctgagc ctgaaatgat caaagtccgt aacttaggac gtaaatcatt agaagaagtt
    45661 aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag aagattaaaa ccttttacga
    45721 aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca catctagcca acgtaaagcg
    45781 atgttgcgtg atttaacaac tgatttatta atcaacgaac gtattgtgac tactgaagct
    45841 cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca ctttaggtaa acgcggagat
    45901 ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa
    45961 gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac tgttcaacga tattgcacct
    46021 cgttacgcag aacgtcaagg cggatacaca cgcattttga agacagaacc aagacgcggt
    46081 gatggtgcac caatggttgt cttagaactt gtctaaaatc acactaataa catcacttta
    46141 tagatgattc ttttagagtg ttatgatgtt ggaagatatt tttccgagtc tagctcagcg
    46201 ctgctcctca agcgaatatc gcttggggag taagcactca tcataaagtg tttttttata
    46261 ccttcaatta cctgtcatga cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga
    46321 aataattgaa aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg
    46381 agtaatcatg gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga
    46441 tcttcgcccc gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat
    46501 tattggacac aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct
    46561 gccagaatca gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac
    46621 gatccgacag atggttggaa tggtttttca aaatccagac aatcagtttg ttggttcgac
    46681 agtagaagat gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct
    46741 agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc
    46801 tgctcgttta tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg
    46861 acctgatatc attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga
    46921 agtgatctct acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac
    46981 tcatgatatt gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact
    47041 tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg
    47101 tctagatcta ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt
    47161 gcccagtgaa tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa
    47221 caagtagact tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc
    47281 aatatgacga tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa
    47341 tcaaccttac ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt
    47401 ggagaacgag atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa
    47461 gttggcatcg tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac
    47521 attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa
    47581 gaaacattag aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt
    47641 tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta
    47701 cttgtattgg atgaaccaac ggcaggactt gatccccagg gcagaaaaga aatgatggag
    47761 atgttttggc gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg
    47821 gatgatgtag ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat
    47881 agcggggagc cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc
    47941 gtacctactg ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc
    48001 ttgcctttga cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc
    48061 catgatgaat aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt
    48121 ggatccccga gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa
    48181 taattgggaa acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa
    48241 agtagatttg aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac
    48301 tgtagctttg caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt
    48361 ccatatcact gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat
    48421 catttttatg tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat
    48481 tgagtacttg cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat
    48541 gctgtcgatt gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa
    48601 cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc
    48661 agtcattcct ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc
    48721 gactgctatg gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt
    48781 gcattggcat aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact
    48841 gatcttttta agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg
    48901 tttgcaagaa aggatgcaag cagtgtatga caagaaatat tagggtctgt ggcagactaa
    48961 ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga aaaaatttta tagaaaaatc
    49021 attattccag ataacctttt aggaacaaag gagagaaaaa catggtacgt tacaaagcaa
    49081 tcattgcgta cgacggaaca aattttaatg gattccaaaa acagccaaat gggcgtactg
    49141 ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa atcacggtct
    49201 ttggttcggg aaggacagat gccggagtac atgcgatcgg gcaagtaatc cattttgatt
    49261 atccagagga gcgtccgtta gaacgaatgc gtttcgcgct agatacccag tcaccagagg
    49321 atattgctgt aagacaagta gaaattgttt cagaagactt ccacgcaaga tatttggtca
    49381 aagaaaagac gtatcaattt cgagtggata tagggaaacc acgcagtcct tttcgacgcc
    49441 attatgcaag ttattttcct tacccacttg atttggaaaa aattcagcgg gcgcttcctg
    49501 atttattagg gactcacgat ttcacctctt tttgtgcatc aggaagctcg atagaggata
    49561 aaatacgtac gatctacgag gctaagatgg aggtaaatga aacaaaagac gaattgttgt
    49621 ttacatttag gggaaatggc ttcttgtata agatgatacg gatcttagta ggaacgcttt
    49681 tgaaaattgg caatggccga ttgcctgaag atagtatccc agatattatt gcgaaaaaag
    49741 atcgtaatgc agcaggacct acagcacacc ctgaaggact ttatttgtat gaagtgaaat
    49801 atgaataaga ggttatgata gaagtggtca gcttcaagaa ataagaaggt atttacgaaa
    49861 attacttctc aaaattttgc aaaatttcgg cttattttcg aagaagctac ttctggaaca
    49921 ccgtttaaga aagaggtcgt gagcctgtca tttaactctg agtcacaaag agcaatttca
    49981 aaaaacagct tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga
    50041 atcatctttt tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt
    50101 gcccttggtg cggacagtct gaacaggttc catcgtttct ttattttcta cgtcttcttc
    50161 tgtgtgtgtt tcgtggttgt tcttctattg ctggatcaat cgtgccataa aaggggcgat
    50221 tgcggtcaag gtaccagaca taaagaataa acaatgcaat acataggaca aaaaggattg
    50281 cacctatcgt aaatccttca ggcaagtaac tgaaagtgac agtatgttta cctgctggaa
    50341 cttttatcat caagaatgcg tctttgaaat tgctgacctt gactggtttc ccatcaattt
    50401 tagctttcca tcctttgtca taaggaatag ttgtcaccaa catggaatct tcagcggtat
    50461 ttacagtccc agaagctttt ctgccagtgg cttccatttc gacacctttt tctttgatct
    50521 gattcattgc ggcttgatag gctacaacat ctagaccaac gactttaggc tccatgaaac
    50581 ttatttcttt cgtcccataa aaacttgcag taaatgtcac tgttgtctct tgaggataat
    50641 agccaagatc atagtattga ccagaaatgt tgatttgcgt ttttcgcgat gtaccattga
    50701 cagtgatcgt cgcactagaa ctttctaatt gcgcaaaatt ggttgggaac aaacttaagt
    50761 aagcttgtgt atttgcaggc acatggactt cccaagtgag ctcttttggc tggttatctg
    50821 ctgtttctga gtaagtgacg ccagtcgttg tctttgtgat cgtcgtattc aactgattct
    50881 tcaaagtagg tgtatagaat gtaaaatact gttcatttag accagacaat gtattgatca
    50941 agtttgtctg actggtcaaa ttatctgtca ctggctgctc aacatcatta acggacaacg
    51001 gtgcagtaaa tgctagtggc aatgcattga cattctgata cagtgtatag tcgccgcttt
    51061 gaccagcttg gcggaagcca tatttactga acgtgctttc gtttttagaa atgttatatt
    51121 tgattcctgt aaacccatcc atcagcaaag tattattgtt ataacggata ttcaaatttg
    51181 tccctcttga acggaaacct aatttatcca ggtaagagga agaattacga tttcttatag
    51241 aagaaaacaa actgatacca ctgtaaccat agttgatcga atcgtttgca gatactggat
    51301 ccagattttc caagcggtaa aacgagtcgg aatctttttt tgtttcattt acaagagctt
    51361 tcaagtcagg ataaggatca gtatataaac tgcgggaagc ataattccaa tcatcaagaa
    51421 tgccatggat catactgttc gtattgacca gtgcttcagc agacatgatc aataataaca
    51481 atatactcag atgattgaat gaaagttttt tatgctgata aaatcccaca gcaaaagcat
    51541 aaatccctaa gaaaaccact gtcaatacaa aggaagtcat cgtcacatag gtgtagcttc
    51601 cttcaggttt gatgccccat gccaccgcaa agagagcagc tacgaccaaa atgattccta
    51661 acaacagccc acgatcgtct tgttctatct tttcccatcc ttttcctgca agcaggagca
    51721 ctaagaaaga gaacagatag gcataacgga ataaaaacat attcggtgca tgcataccat
    51781 gccagaacaa attcaatgga acaagataaa aacttgcgat aagtatggcg aaaagactgc
    51841 cgtacaaaaa tttatttttc aacggtactt tccgactggc aaaataaaac agacagagga
    51901 ctaatggtag cagacctgcg taaacgaacg gaatcgatcc gtattttgtc gtgtcataaa
    51961 caccaatcat atttttcatg attagatcca gataagaagt cgcttctgtt ttcaacgtag
    52021 tgatctgact cagtgcctcg ccatttgacc gaagatccag cactgctggc aatacaatga
    52081 tcattgaagc gccgcctgcc agtaaagaag tgataccata aggaacaatc gtttttttat
    52141 attctgacca gtttgtcaat agacgggcga taaaatataa gaaagaaaat acgccaatca
    52201 taaaacccat ataaaaactg gataaaaaca agagtaaata actgataaat aaaagtttcg
    52261 gccgtttaag atccataaca cgatgaatcc ccaaaatcac taatggaaga taaacaaatg
    52321 catccagcca catgatgatc tccgaatgtg cagtgataaa tgagatcaat gcgtaactga
    52381 cgctaagtgc aacatgcccc cagcgaggaa tcttgaacgt acgtgcaaga aaccaaaaag
    52441 ccaatccggc acagccgacc ttaagcaaag tcagaacata cagtgcatct ggcatcatct
    52501 ggtttggaaa aaagaacacc agtggcgtga agatcccgcc tagatagtaa gaaattaaag
    52561 ataaataatt caatcctaga gaagcattcc atgtataaaa aatactttgc tttccaagaa
    52621 gaacatttcg gaagctggca tgaaaattag aaaactgtga aaaggcatca cttgccaaaa
    52681 tacttctgct gcttccagga tagattccga tacttagata aacaagtgct aaaatcaaaa
    52741 acggaatcag gaaactagct gctgcgtagc gtccgttttc tctaaagaat gactttaaat
    52801 tttttttcat atttattttc caccaaattt ctatgaagtt gtattcagtt tatcatatcc
    52861 ttgtttttct ctgcttaaaa agagtctagt tttcacaaaa aaataaaatt tagaataaat
    52921 aacttcataa tttgttcgta taacaaaaat aaacgcaaaa aaagggtagg aatcaaaagt
    52981 cccaccctta aaaattttta ttaaataaaa aaccgttcac tatctgaacg gtccaagtat
    53041 cattcaatta aacgcgttca acttttcctg atttcaaagc acgagttgac acccaaactt
    53101 ttttaggttt tccgtcgacc ataacgcgaa ctttttgtaa gttaggttta acagtacgtt
    53161 ttgttgagtt catagcgtgt gaacggttgt ttccactttt tgtcttgcga cctgtaaagt
    53221 aacaaacttt tgccatggta atttcctcct ttgccttgat ttggagcaat agaataattc
    53281 agctcatacc agactaacat atcatataga atgggaattt gcaagtacat tttcatagat
    53341 acaattgaca attgagaaga ataattgtaa attaaacaag gttatacctg aaattttgca
    53401 aggtttttga gtactttggg taactgcctt ttatttcaag ttgcgctatg ataaaatatg
    53461 atagaagaaa aatgggagtt ttttacccgt taaggaggct cattcatggc tgtaaaaatt
    53521 caaacgtcag ctggaaccat tgaaattacc aatgatgtaa ttgcaacagt tgttggtggc
    53581 gccgcgacag atgtgttcgg tgttgtagga atggccagca aaaatcaaat taaagataat
    53641 attactgaga ttttgcgtaa agaaaattac tcaagaggag tagttgtccg ccaagaagat
    53701 aatggtattg ctgtagatgt ttatacgatt atcagctatg gtacgaaaat ctctgaagtt
    53761 tcccgcaacg tccaagaaaa agtgaaatac aatctagagg cattattagg cgtaactgcc
    53821 aattcagtta atgtctttgt acaaggtgtt cgtgtactgc cagactaggc aggagactgg
    53881 ctggtaactc agcagtcgat ctgaaggagg attatctagg tgagtgtaac agaaataagt
    53941 gcaagccagt tccaggaaat ggtccaggca ggtgcgaatc gtctgcaagt aaatgcagaa
    54001 tacgtcaatt cattaaatgt attccctgtg ccagatggcg atacgggaac aaatatgaat
    54061 ctatctatga ctagtggagc taaggcagta gtagattcaa cctcagaaaa agtaggagaa
    54121 ttagcagcaa tgctttcaaa aggattgctg atgggtgcgc gcggaaattc cggcgttatt
    54181 ttgtctcaat tgtttcgtgg attttctaag caaattccag atgttgtctc attaaatgct
    54241 tctgatcttg ctgcagcctt cactcatgga gttgaaactg cgtataaagc agtaatgaaa
    54301 ccagtagaag gaacgatttt gacagtagct agagaagcag cgaaagctgg agaaaagaaa
    54361 gcaaaaagta ctgatgatgt cgttgaagta atgacagctg ttgtaaaagg agcaaagcgt
    54421 gctttagcta aaactccaga cttgcttcca gtcctaaaag aagtcggtgt cgtagacagt
    54481 ggagggcaag gactgctgtt tgtttatgag ggctttttaa gtgcattgaa tggagaattc
    54541 caagcggatg aaacatatga accttctcca gcagaaatgg atgatatggt caatgcagaa
    54601 catcatcgca gtgtccaagg tcaaatggcg acagaagata tccattttgg ttactgtaca
    54661 gaaatcatgg taaaaatcgg agaaggacct acagtcgata gtacattcga ttacgaagaa
    54721 ttccgtaact atttagatgg tataggagat tctttacttg tggtcaatga tgacgagatc
    54781 atcaaagtcc acgtgcatac agaaaaccca ggagaagtct tgaattatgg acaaaaattc
    54841 ggttcattag tcaaaatcaa agtcgacaac atgcgcttgc agcatgagac gattttggaa
    54901 cacgatgaag aagtgaacga gtttgcgaaa aaagaaatac cacgtatccc gtatggtatc
    54961 gtagcgatcg ctgctggtaa aggcgtccaa gagctgtttg aaagtttagg tgctaattat
    55021 gttatcagtg gtggacaaac aatgaatcca agtacggaag acattgttaa agcgattcaa
    55081 gaagtaaacg cagataaagt gattgtcttg ccaaacaata agaacatctt catggcagct
    55141 gatcaagcag cagaagtagc agatatccca gtagcagttg ttccttcaag aacgatttcc
    55201 caaggattga cagctatgct ttcattcaat gatcaagctt ctttagaaga aaacaaagaa
    55261 acgatgacag aaatgttgtc tagcgttgtg agcggtcaag tcactcacgc gatcagagat
    55321 acctcaattg acggtgtaga gatcaaagaa ggagactttt tgggcatgat cgatggaaaa
    55381 atcctgatct ctaacccaga tatcacagca acagcgttaa gtacactaga acaaatgatc
    55441 aatgaggaca cagagatcgt aacgatcttg gcaggtgatg aaggaacagt tgaacaagca
    55501 aatgaatttg ctgagaaact aacagaaaaa catcctgatt tggaaatcga gatccaccaa
    55561 ggagatcagc ctgtctatcc ttatctattt tctgctgaat aatttctgtg tcagaatatc
    55621 tttctaacac agtattcatt cataaaaggc cttcggttag tgatcaggta tgatcgctga
    55681 ccaagtcctt tttttgttaa aggagggagg aaaaatttat gcgttcttta gatgatccaa
    55741 tcactatgct ggcaggagtc ggagaaaaaa gagcagctag tcttgccagc ttgggcatcc
    55801 aaacgattga agacttactg acttattatc cttttcgcta cgaagatatt caggaaagaa
    55861 atctaaacga gatccaggat caagaaaagg ttactttgaa aggaatcgta gtctcgcctc
    55921 ctgtgatgaa tcgatttggt tataaaaaaa gccgattgca atttcgtatg atgcaagaac
    55981 atgatgtgtt caacgtttct ttttttaatc aaccttatct aaaagaaaaa gtcattttat
    56041 cggaggaaat cgccgtatac ggtaaatggg atgcaaagcg taaagccttg aacgggatga
    56101 agattttagg ttctcaaaca atggatgact tttcgccgat atatcacgtc aacaaatcaa
    56161 ttcgtcaaac gacattagtt gatctaatca aaaaagggtt tcaagaatac ggcacattga
    56221 ttgaagaaaa tctgccaaac aaccttgtgg agaaatatcg actgcttgac cgagcttctg
    56281 ctgtaagaag tatgcatttt ccaaaagacc acgaagaaaa ccatcaagcc aaacgacgtg
    56341 tcgtttttga agagttcttt ttatttcaaa tgcgtcttca aggattgaaa aaagcagaaa
    56401 aagcagaaac gaatggtttg gagatccttt atgatgtcca aaaattgaaa caatttactc
    56461 aaaagctacc ttttgaactg acaaatgcac aaaaacgtgt aacgaatgaa atctgtcggg
    56521 atcttcgcag tccgcagcat atgcagcgac ttttgcaggg agatgtaggt agcggaaaaa
    56581 cagtggttgc cgcgattgcg ttatacgcta ccgttacagc aggtttccaa ggtgcgttga
    56641 tggtgcctac agaaatcttg gcacaacaac atatggaaag cttgcaacag ctgtttgatc
    56701 ccaatgaagt aaccatcgca ttgattacgg ggtcaacgaa agcaaaagaa cgacgtgagc
    56761 tccttgaaaa actagagcaa ggggaaatta acgtcgtgat cggcacacat gcattgatcc
    56821 aagatggagt cgaatttcag aatttaggtc tagttattac ggatgagcag caccgttttg
    56881 gcgtgaacca gcggaaagta cttcgtgaaa aaggctggcg accagatgtc ttatttatga
    56941 cagcgactcc gattcctaga acattagcta ttacagcata tggtgagatg gatgtatcta
    57001 tcattgatga acttccagct ggacgtatcc cgatcgaaac acgctgggta agaacacctc
    57061 aattggacag tgtactggat tggacgaaaa aagaactggc aagaggacat caaatgtatg
    57121 tcatctgtcc gctgattgaa gaatcagaag cattagatgt aaaaaatgca gtggaaatct
    57181 atgaaaaact tcgtgaacta tttgcaccaa aatatgaagt tggcttgtta catggtaaaa
    57241 tgaagaatca ggaaaaagaa gtaatcatgg aagccttcaa agacaaccag atgcagatat
    57301 tagtttcgac tactgtaatt gaagtagggg taaacgtgcc gaacgcgacc gtcatgctga
    57361 tcatggatgc ggatcgattt ggattggcac agctgcacca acttcgtgga cgtgttggac
    57421 gcggaagtga tgcttcctac tgtatcttag ttgctaatcc taaaaatgaa ctaggtgtgg
    57481 aacggatgaa aatcatgaca gagaccacca atggttttgt actaagcgaa aaagatctgg
    57541 aattacgtgg tcctggtgaa gtatttggct tccgtcaatc tggtctgcct caatttgcga
    57601 ttgctgactt ggtgacggat ggaaatgttt tggaggtagc cagagaagag gctgcagccc
    57661 tttggcaaat aaaggattgg cagatgcttc ctgagtatca gcctttagcg caaagcctcc
    57721 aaatagaaaa tcaggataat cgttatttcg attagcagac tgtgctatac tttatactga
    57781 tgacggaaaa aggagtggaa acaatgaaaa ttgccgtaga tgcaatgggc ggagatcacg
    57841 cacctcaagc aattgtagaa ggcgtggctt tagcgcagca agattttcca gaagtagaat
    57901 tcttacttta cggaaaagaa gcagaaatca agaaatactt aacaaatgaa aaaaacatca
    57961 cgattatcca tacagacgaa aaaatcaata gtgatgatga gccagtaaaa gcaattcgac
    58021 gaaaaaagac tgcatcaatg gtccttgctg cacaagcagt gaaaaacggt gaagcagatg
    58081 ctgtattttc agcagggaat acaggcgcgc tactagcagc agggttgttc atcgtaggac
    58141 gtatcaaaaa tatcgaacgc cctggcttga tgtctacctt gccagtagta gggaaagaag
    58201 gtgccggttt cgatatgctg gatcttggcg ctaacgcaga aaacaaacca gaacatttgt
    58261 tgcaatacgg tattttaggt tctttttatg cacgtaaagt ccgaggtatc ggacgtcctc
    58321 gtgtcgcact cttaaacaac ggaacagaag caacaaaagg tagtgaagtg acgaaaaaag
    58381 cctatgaact ccttcagaat gaaacaagct tgaattttat tggaaacgta gaagctcgtg
    58441 agctactcaa tggtgtggct gatgtagtgg tcacagatgg atttacaggc aacgctgttt
    58501 tgaaatctat tgaaggaaca gcaatgaata tgatgaatct attaaaatct gctattctaa
    58561 atgaagggat caaaggaaaa atgggcgcta tgcttttaaa agacggctta cgttctttga
    58621 aagcagaaat ggattattcc aaacatggcg gtgccgtttt gtttggcttg aaagcaccag
    58681 tcattaaaac tcatggagca acaggccccg atgctgttcg ctatacgatc cgccaaatcc
    58741 acacaatgct tgagacagat gtagttggac aattagtcga acaatttgaa aaaagcgaag
    58801 aatagtattt ggatatctga agcgatccgt tccattgaaa aaaaggtgga cattcctgaa
    58861 aaatgccagt aaaattagcc atggtgtagg taattgacaa gtgtatgatt cttggaggtg
    58921 aaaaagttga ctcgcgaaga agtatttaac aaagtagcga aaattatttc aaatcatttt
    58981 gagatagata ccgataaagt gacggatgaa ttgaacatca aagatgattt aaaagctgat
    59041 tcaattagta ttatggaatt cgttttagag cttgaagatg aattcggtac ggaaatttca
    59101 gatgaagacg ctgaacaaat tgaaacagtc ggtggcgcag tagattatat cagcagccat
    59161 cttaaataat ttcaaataaa agcaaagaag gataaaaagc tgcatgcagc tttt
//
LOCUS       DACDVT010000010        59007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDVT010000010 DACDVT010000000
VERSION     DACDVT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.010"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..596)
                     /locus_tag="IWL52_000600"
     CDS             complement(<1..596)
                     /locus_tag="IWL52_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9777186.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
     gene            789..1820
                     /gene="queA"
                     /locus_tag="IWL52_000601"
     CDS             789..1820
                     /gene="queA"
                     /locus_tag="IWL52_000601"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9777187.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            2439..5078
                     /locus_tag="IWL52_000602"
     CDS             2439..5078
                     /locus_tag="IWL52_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9777188.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(5246..5944)
                     /locus_tag="IWL52_000603"
     CDS             complement(5246..5944)
                     /locus_tag="IWL52_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9777189.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            6162..7307
                     /gene="tgt"
                     /locus_tag="IWL52_000604"
     CDS             6162..7307
                     /gene="tgt"
                     /locus_tag="IWL52_000604"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9777190.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            7404..7784
                     /gene="yajC"
                     /locus_tag="IWL52_000605"
     CDS             7404..7784
                     /gene="yajC"
                     /locus_tag="IWL52_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9777191.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            8089..10686
                     /gene="adhE"
                     /locus_tag="IWL52_000606"
                     /gene_synonym="adhC"
     CDS             8089..10686
                     /gene="adhE"
                     /locus_tag="IWL52_000606"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9777192.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            10915..12021
                     /locus_tag="IWL52_000607"
     CDS             10915..12021
                     /locus_tag="IWL52_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9777193.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            12038..13066
                     /locus_tag="IWL52_000608"
     CDS             12038..13066
                     /locus_tag="IWL52_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9777194.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            13072..13551
                     /locus_tag="IWL52_000609"
     CDS             13072..13551
                     /locus_tag="IWL52_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9777195.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            13567..14862
                     /locus_tag="IWL52_000610"
     CDS             13567..14862
                     /locus_tag="IWL52_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9777196.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            14873..15697
                     /locus_tag="IWL52_000611"
     CDS             14873..15697
                     /locus_tag="IWL52_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9777197.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            15862..16761
                     /locus_tag="IWL52_000612"
     CDS             15862..16761
                     /locus_tag="IWL52_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9777198.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16887..17756)
                     /locus_tag="IWL52_000613"
     CDS             complement(16887..17756)
                     /locus_tag="IWL52_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9777199.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            17909..19369
                     /gene="rhaB"
                     /locus_tag="IWL52_000614"
     CDS             17909..19369
                     /gene="rhaB"
                     /locus_tag="IWL52_000614"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9777200.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            19366..20622
                     /gene="rhaA"
                     /locus_tag="IWL52_000615"
     CDS             19366..20622
                     /gene="rhaA"
                     /locus_tag="IWL52_000615"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9777201.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            20636..21481
                     /gene="rhaD"
                     /locus_tag="IWL52_000616"
     CDS             20636..21481
                     /gene="rhaD"
                     /locus_tag="IWL52_000616"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9777202.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            21483..21794
                     /gene="rhaM"
                     /locus_tag="IWL52_000617"
     CDS             21483..21794
                     /gene="rhaM"
                     /locus_tag="IWL52_000617"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9777203.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            21876..23027
                     /gene="fucO"
                     /locus_tag="IWL52_000618"
     CDS             21876..23027
                     /gene="fucO"
                     /locus_tag="IWL52_000618"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9777204.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            23154..23912
                     /locus_tag="IWL52_000619"
     CDS             23154..23912
                     /locus_tag="IWL52_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9777205.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            24012..24656
                     /locus_tag="IWL52_000620"
     CDS             24012..24656
                     /locus_tag="IWL52_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9777206.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            24663..25670
                     /locus_tag="IWL52_000621"
     CDS             24663..25670
                     /locus_tag="IWL52_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9777207.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            25686..26528
                     /gene="kduI"
                     /locus_tag="IWL52_000622"
     CDS             25686..26528
                     /gene="kduI"
                     /locus_tag="IWL52_000622"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9777208.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            26548..27351
                     /locus_tag="IWL52_000623"
     CDS             26548..27351
                     /locus_tag="IWL52_000623"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9777209.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27616..28956)
                     /locus_tag="IWL52_000624"
     CDS             complement(27616..28956)
                     /locus_tag="IWL52_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9777210.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(29142..30206)
                     /locus_tag="IWL52_000625"
     CDS             complement(29142..30206)
                     /locus_tag="IWL52_000625"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9777211.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(30203..31288)
                     /locus_tag="IWL52_000626"
     CDS             complement(30203..31288)
                     /locus_tag="IWL52_000626"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9777212.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(31301..32278)
                     /gene="mvaD"
                     /locus_tag="IWL52_000627"
     CDS             complement(31301..32278)
                     /gene="mvaD"
                     /locus_tag="IWL52_000627"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9777213.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(32271..33215)
                     /gene="mvk"
                     /locus_tag="IWL52_000628"
     CDS             complement(32271..33215)
                     /gene="mvk"
                     /locus_tag="IWL52_000628"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9777214.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            33537..34190
                     /locus_tag="IWL52_000629"
     CDS             33537..34190
                     /locus_tag="IWL52_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9777215.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            34275..35180
                     /locus_tag="IWL52_000630"
     CDS             34275..35180
                     /locus_tag="IWL52_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9777216.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(35182..35814)
                     /locus_tag="IWL52_000631"
     CDS             complement(35182..35814)
                     /locus_tag="IWL52_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9777217.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            36134..36658
                     /gene="infC"
                     /locus_tag="IWL52_000632"
     CDS             36134..36658
                     /gene="infC"
                     /locus_tag="IWL52_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9777218.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            36730..36930
                     /gene="rpmI"
                     /locus_tag="IWL52_000633"
     CDS             36730..36930
                     /gene="rpmI"
                     /locus_tag="IWL52_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9777219.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            36983..37342
                     /gene="rplT"
                     /locus_tag="IWL52_000634"
     CDS             36983..37342
                     /gene="rplT"
                     /locus_tag="IWL52_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9777220.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            37594..38931
                     /locus_tag="IWL52_000635"
     CDS             37594..38931
                     /locus_tag="IWL52_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9777221.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            38979..41108
                     /locus_tag="IWL52_000636"
     CDS             38979..41108
                     /locus_tag="IWL52_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9777222.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            41083..41772
                     /locus_tag="IWL52_000637"
     CDS             41083..41772
                     /locus_tag="IWL52_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777223.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(42180..42866)
                     /locus_tag="IWL52_000638"
     CDS             complement(42180..42866)
                     /locus_tag="IWL52_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9777224.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(43002..43196)
                     /locus_tag="IWL52_000639"
     CDS             complement(43002..43196)
                     /locus_tag="IWL52_000639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777225.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(43246..43686)
                     /locus_tag="IWL52_000640"
     CDS             complement(43246..43686)
                     /locus_tag="IWL52_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9777226.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(43690..44535)
                     /locus_tag="IWL52_000641"
     CDS             complement(43690..44535)
                     /locus_tag="IWL52_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9777227.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            44684..46102
                     /gene="pepV"
                     /locus_tag="IWL52_000642"
     CDS             44684..46102
                     /gene="pepV"
                     /locus_tag="IWL52_000642"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9777228.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(46159..47106)
                     /locus_tag="IWL52_000643"
     CDS             complement(46159..47106)
                     /locus_tag="IWL52_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9777229.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(47329..47679)
                     /locus_tag="IWL52_000644"
     CDS             complement(47329..47679)
                     /locus_tag="IWL52_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9777230.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            47879..48958
                     /gene="pepA"
                     /locus_tag="IWL52_000645"
     CDS             47879..48958
                     /gene="pepA"
                     /locus_tag="IWL52_000645"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9777231.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            49102..49422
                     /locus_tag="IWL52_000646"
     CDS             49102..49422
                     /locus_tag="IWL52_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9777232.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            49444..49908
                     /locus_tag="IWL52_000647"
     CDS             49444..49908
                     /locus_tag="IWL52_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9777233.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            50113..50718
                     /locus_tag="IWL52_000648"
     CDS             50113..50718
                     /locus_tag="IWL52_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9777234.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(50757..52046)
                     /locus_tag="IWL52_000649"
     CDS             complement(50757..52046)
                     /locus_tag="IWL52_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9777235.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            52474..52953
                     /gene="rlmH"
                     /locus_tag="IWL52_000650"
     CDS             52474..52953
                     /gene="rlmH"
                     /locus_tag="IWL52_000650"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9777236.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            53038..53547
                     /locus_tag="IWL52_000651"
     CDS             53038..53547
                     /locus_tag="IWL52_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9777237.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            53647..54297
                     /locus_tag="IWL52_000652"
     CDS             53647..54297
                     /locus_tag="IWL52_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777238.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            54748..55686
                     /locus_tag="IWL52_000653"
     CDS             54748..55686
                     /locus_tag="IWL52_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9777239.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            55699..56751
                     /locus_tag="IWL52_000654"
     CDS             55699..56751
                     /locus_tag="IWL52_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9777240.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            57017..57652
                     /locus_tag="IWL52_000655"
     CDS             57017..57652
                     /locus_tag="IWL52_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9777241.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            57843..58634
                     /gene="msrA"
                     /locus_tag="IWL52_000656"
     CDS             57843..58634
                     /gene="msrA"
                     /locus_tag="IWL52_000656"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9777242.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        18647 a        10587 c        12643 g        17130 t
ORIGIN      
        1 gcttcatcca tgtcatgggt tacaaatacg atcgtttttc ccaaacgctc ttgtaaatct
       61 ttgacaaggt cttgcaagga atcacgagtg attggatcaa gcgcaccgaa tggctcgtcc
      121 atcaagatga tatcttgatt cgctgcaagg gcacgaacga ctccaatacg ctgctgctga
      181 ccaccagata attcatgtgg gtagcgatct aacatctcac ggggcaattc cacaagatca
      241 atcatttttt cagctgtttt attccgttct tcttgactga ctttcaacaa tttttggaca
      301 agagtgatat tttcacggat cgtcatgtga ggcatcaatc caatactttg gataacatag
      361 ccgatctgac gtcgcaattc cactggatca atttcttgga tggattttcc atcaatcaaa
      421 attttccctt gtgttggatc agtcattcgg ttgatcatcc gcatcgtcgt cgtttttccg
      481 cttccgctcg taccgataaa acaaatgaac tcaccctttt caaatgaaag attgacgtcc
      541 tctacagcga cttttccacc tttataaatt tttgacacat tttgaaattc aatcatggtg
      601 ccacctcttt ttctattttc aatgaagaac tatctattac tagtatgcct aaaacaatga
      661 aaaaatacaa atagggtagt ttttttattt aggaaaacaa aaaatagaag tgaaaaagtg
      721 ctgattttgc caacagaacg aaaaacatga tagaatgatg aagcacatga tagaaagtga
      781 gtgacgatat gttaacaaca gaagattttg attttgactt gccggaagaa ttaatcgcac
      841 aaacaccatt aaaagaccgc gatcattctc gtttacttgt tttggatcgg aaaactggag
      901 agatggaaga taaacatttc catgaaatca ttgaagagtt acagcctggt gatgcgctag
      961 ttatgaataa tacacgagtt ctgcctgctc gtttatatgg agaaaaacca gatactggtg
     1021 cgcatttaga agtccttctt ctaacgaata cacaaggaga tacgtgggag acattgatca
     1081 aacctgcgaa acgtgccaaa gttgggacaa gaatctcttt tggtgatggc agattgcaag
     1141 cagtagtgaa agaagaattg gaacacggcg gacggatcat tgactttgag tatgaaggaa
     1201 tttttctaga gattcttgaa tcattgggag aaatgccatt gcctccatat atcaaagaac
     1261 gtctggaaga cccagatcgc tatcaaactg tctatgcgaa agaaaatggt tctgctgcag
     1321 cgccaacagc tggattgcat tttacgaaag aattacttga tgagatcgaa gcaaagggtg
     1381 ttgaattagt ctatctgaca ctacatgtag ggttaggtac attccgacct gtcagcgtag
     1441 ataaaattga agaacaccat atgcatagtg aattctaccg tttgacggaa gaagcggctg
     1501 agcgattgaa ccaagtacgt aaaaatggcg ggaaaatcgt ggctgtcgga acaacttcaa
     1561 ttcgaacatt agaaacgatt ggaacgaaat ttaatggaga aatcaaagca gacagcggat
     1621 ggactgatat tttcattacg cctggctatg aattcaaagt ggtagaagct ttctcaacta
     1681 atttccattt gccaaaatcg actttagtca tgttagttag tgcctttgca ggaagagaat
     1741 tgactttatc tgcttaccag catgcgattg aagagcgcta tcgtttcttt agttttggag
     1801 atgcgatgtt cgtgaaataa ggttatgaaa agccgctagt tttgaataac aaaaaagctt
     1861 tacaaagatt ttatgacaaa gaaaaagccg agctctcgat aagctcggct ttcttgtacc
     1921 tcggaaggat tcaaataaac agaaatgatt gtaactttcc atcatacatg caaagcacgg
     1981 tgttgaattt cttcatgtta aacaaaaatt ttgttttcgt tacatcttca acatttgaaa
     2041 catctcttgt tcctattcaa ttttcagacg tttacgttat tcaattgtta cgtatcgcat
     2101 tacttcttgt ttcggatact tcgtgatcca aacatccata cgcattcact atttataggt
     2161 ttgtactcta cacgcatgtc tgggtgaaac ctgtacttct aacaactttg tttgcttaca
     2221 ctgttctatc gagaaattct tctgtctatt tgatggtatc ctccttcctt acatctatat
     2281 aatagcatcc tttggtagcg tttgcaaata taagcacttt aaattttacg ctttcttaac
     2341 aaataatcga taagcccttt atttgaagta aataaaaaaa ggatgttata gtaaaataag
     2401 tacctttttt tgtacgaaga ttttaaagga ggaataggat ggaagaatac aagcagacaa
     2461 tagaagaagt aaagaaggta acagaagcag atccagatac tgggttgtct tcacaagaag
     2521 tggatgaacg gcgaaaaaag cagggattga ataaattcga tgaggcgccg aaagaatcga
     2581 tgatcaaaaa attctttcga agcttgtccg attttacaac gatcatcctt cttgtagcag
     2641 cggtgatttc cttttatacc gcatttgcta cagaacatgg ggatttattt gaaggactgc
     2701 tgatcatcgc tatcgttgtg atcaactcgg tattggcgat tgtccaagaa ggaaacgcag
     2761 aaaaagcact ggaatccttg caagatatga ataaacaaac ggcaactgtc atacgagacg
     2821 gcaaagtgac cactgttgag tcagagcaac tggtagtggg tgatattctt gttttagagt
     2881 ccggtgatgc tatttctgcc gatgcccgtt tgatcgaagc atcacaatta cgtgtagaag
     2941 aatcggcgtt gactggagaa agtgaagcag ttgaaaaaga tgcagcattt attgctgaag
     3001 aagatgagtc attaggtgac caacttaata tggtatttaa aggatgtacg gttgctgcag
     3061 gtcgtgggaa agcgatcgtc actgccattg gcatggctac agaaatgggt aagatcgcgg
     3121 atcttttgaa cgaaaatact atgcaaaaaa cacctttaca ggtccgattg aatcagctag
     3181 gcaaacgaat cagtatgatc gctttagcgg ctgcggcttt agtatttgtg attggtgaac
     3241 tgcaaggaga accactcctt gagatgttca tgacttcgat ttccttggct gttgcagctg
     3301 taccggaaac cttgactgtt attgttacgt tgacacttgc atacggtgtg cagaagatgg
     3361 cgaggaaaca tgcgatcatc cgacagttgc cagcagttga aacgctagga accgctaatg
     3421 ttatctgctc agacaaaaca ggaacgctga cacaaaatga aatgcgcgtc cgccgggtat
     3481 ggagcaaaga agatgaagta acgaatatcg aagattcaat gacgaacagc gcgatggaaa
     3541 tattgaaaat ggctgcacta tgtacagatg tgacggtcga gcaagaagat gacgaccttg
     3601 ttatcaaagg taatccaaca gaagtagcca tcgtaagggc tgttgaagag aattatcata
     3661 caaaggcaga attagaagaa aaatatccac gagtaaacga gcttcctttt gattctgaac
     3721 gaaaaatgat gacaacagtt caccaaatgg gcgaaaaata catctcaatc accaaaggag
     3781 cattcgatgt tcttgctccg cgctttagtt caggcgatgt agaacaagcg gcgatcgtta
     3841 atgatcgctt tggtaaacgg gcactgagag tgattgctgt tggttatgct acttatgacg
     3901 aagaaccgca agatatttct tctgaagcac tggaaaaaga cttacgtttg attggtctga
     3961 tcggaatgat cgatccacca cgtccagaaa gtaaaggagc aatcaagcga gcgaaaaagg
     4021 ctggcatcaa aacagtcatg atcactggag atcatgtggt gactgccagt gccatagcga
     4081 aagaattagg tatcttgaat gatccgtcag aagcactttc aggatcagaa ctgcatcaat
     4141 tatcagacga agaattagat gcgcgtgtca aagctctctc tgtctatgct cgggtaacgc
     4201 cagaagacaa gatccggatc gtcaaatctt ggcaacgtac gggtgcagtc gtagcgatga
     4261 ctggggatgg tgtgaatgat gcaccagcgt tgaaagcaag taacgttggg tgtgccatgg
     4321 ggatcacagg aacagatgtg gcgcaaagtg ccgcagatat gattttgacg gatgacaact
     4381 ttgcgacgat cgtagacgct gtcagtcaag gacgttcggt ttatcagaat attcgtaaag
     4441 ctatcaactt tttgctaagc tgtaatattt cggagatctt tattgttttg attgcgatgc
     4501 tgttaggatg gggagcacca tttacagctg tgcaactact gtttgtcaat gtagttgctg
     4561 atggacttcc aggatttgct ttaggaagag agccagcgga acacggcatc atggatcagc
     4621 cacccattcc gaaaaatgaa gggatctttg ctcgaggttt attgcaaaaa attgccataa
     4681 atgcaggggt cttcaccatc gtaaccttgt ttggttatta tttaggtagt tatgtcgata
     4741 ctatcagccc atgggtagac gcaagtcagc atgtcggtca aactgttgca ttcttgatct
     4801 tagcttattc atcgattctg catgtgttca atgtacgcag cagccaatcg atcttcaaag
     4861 tcaatttagc gaccaataaa gctttggtag aaatggcgtt actggcatta gctattacaa
     4921 cggctgttgc tttgctgcca tttacgcaag aactattcgg cctagtacac atcagtttga
     4981 atcactggtt cttggtaggg attttatcta tcgtaccaat cgcggtgaat gaactgatca
     5041 agttccatcg attaccggaa gcagaagaag aggaatgatt aactgtttct aagttgattt
     5101 tctttttact ggaactatac aaaaaggagg tgtgacatga atgttgtcac acctcctttt
     5161 tgctaatggg ctgaatgttt cttttactgc ctctcgataa acggtgttct cctgccaccc
     5221 cctcggtcac aagtcacact ctcttttaaa tatcttttgg caattgttca taaaatgctg
     5281 cttttgttgc agaaatgtat gggctgagcc atagataacc aataccagca gttgctaatg
     5341 ctaagatatg ccagccgata aatgaaagat ctaaccagaa aagttgtcct ttataaccat
     5401 ccatcaattt tcgacttgca gtgatcgtat ccagtacttt aggtgtttgt cctgtttctt
     5461 ggacgatatc gtagtaaata aaatagcttt gagaataaga atagctttta atgattccag
     5521 gaataactaa tagcaaaccc cataatgttg taaagatcga gatcagcaca gctaagctga
     5581 tcactccgcc gataaacaaa cctttgaatc cacgaaatgc atctttgaat ggttcgatct
     5641 gatctttttg tccacgtaaa atatcaagat aagtccaaga aataccgctc atgaacaaag
     5701 cgctaatgat gctggaaata attccgccac cgcctcctga actaccgcct cctgaagttg
     5761 tcgcttctga gacgctcgct gatccatcat atatggtggt ggcaaacaag ataataggaa
     5821 tcataataag aagaatagta gcaataccaa tcaatgtagg gattagattt agtaaaacag
     5881 cttgtcccca acgtcctttt agactctctt ttgcttggcg ttttaattct gcagaagatt
     5941 gcatgatttt tccctccttt tctttaataa caccaatata ccatgtatca aaaaatattt
     6001 ctgtcataat gtttttcttt tttttgttat tcataaaaaa aggataaaaa aaagaatatt
     6061 aaataaagta gaaggaagcc gagggaactt gcaaaaagca cttgaaacga ttatgatggt
     6121 gggagttgtc aagatatttt atatagaaag tagggaaatg aatgacgcaa cctgccattc
     6181 gctatcgttt aatcaagaaa gaaaaacata ctggcgcgcg tttaggggaa ttgatcacgc
     6241 cacacggaac tttccccaca ccaatgttca tgccagtagg aacattagct acagtaaaaa
     6301 caatgtcccc agaagagttg aaagaaatgg gtgctggcgt tattttaagt aatacatatc
     6361 atttatggct acgccctggt gaagacttag tagaagaagc tggcggactg cataaattta
     6421 tgaactggga tcaaccgatt ttaacagatt caggcggctt tcaagttttt tcattaagtg
     6481 atatgcggaa tatcgaagaa gaaggcgtgc atttcaaaaa tcacttgaac ggttcgcgta
     6541 tgtttttatc accagaaaaa gcaatcaaca tccaaaataa attgggctct gatatcatga
     6601 tgagttttga cgaatgtcca ccattcgatg aaagttacga ctacgtgaaa aaatcagtag
     6661 aacgtacgtc tcgatgggca gaacgaggat tgaaagctca tgctaatcca gaccgtcaag
     6721 gattatttgg aatcatccaa ggagcagggt ttgaagatct tcgtcgtcaa agcgcgaaag
     6781 atctagtcgg gatggatttc cctggttact caatcggcgg gttatctgtc ggggaaccta
     6841 aatcagaaat gaatcgtgta ttagaattca ctacaccgtt gatccccgaa aacaaaccac
     6901 gttacttgat gggtgtcggt gctgctgatt cattgatcga tggtgttatc cgtggagtag
     6961 acatgtttga ctgtgtcttg cctactcgaa tcgctcgaaa tggcacatgt atgacttctc
     7021 aaggccgtct agtagtgaaa aatgcgaaat atgcccatga tttccgtcca attgatgaga
     7081 aatgtgattg ttatacatgt aaaaactata cacgtgccta tatccgccat ttgatcaaat
     7141 gtgatgaaac atttgggatt cgtttgactt cttaccacaa tttatacttc ttgttgaatc
     7201 taatgaaaca agtacgtcaa gcaatcatgg atgataattt gcttgaattt agagaagcct
     7261 tttttgaaga atacgggttc aataaagaaa acgcaaaaag cttctaaagg aaaatctttt
     7321 aaaaattaag taaaaaacta gtgttatcag catttttctg ctaaagtgtt ttttgtagat
     7381 acagaattag tgaggtgaaa aaaatgggaa cattaccaat gcttgtgatg tttatcgcgc
     7441 tattagcaat gtggtttttt atgtcacgtt cgcaaaagaa acaacagcaa gaacgtcaaa
     7501 acttgttaga tagcatgaaa ccaggtgatt cagtcgtaac gatcggcgga ttacatggag
     7561 ttgtttcaga aatcgacaat gacaaacgga cagttgtttt agactgcgaa gggatcttcc
     7621 ttgagtacga tcgtgcagca atcaaaacgg tcaaaccagg gactgtcgta acaaatgatg
     7681 caacgactac tgttgaaaca acagaagaac cagctgagac agttgaagta aaaactgaaa
     7741 caacagaaac gctggaagag acagaagaga aggaagaaaa ataagtatca aagcttattt
     7801 aaagacgagg tagggcagaa agatgtccta cctcattttt tattcaaaaa agaaagagat
     7861 aagagcatgg gatatattgc tcaatattga ataatcgggt gtgtcatata aataataata
     7921 aataacttta tatatttttc tatacaaaca cctgtaaata aagcaatttt atatttaaaa
     7981 ataactgtga aaaaaatcac aaaaaagtgt ttacatagaa aaataaatat tgtatgatat
     8041 acatgtgaat tatttaacaa ataaccatga cccattagga ggataatgat ggctaaagta
     8101 atagaaaaag aaaaaacgaa gacaattgac gtacaagcaa tgattgatga tttggcagaa
     8161 aaagcaaatg cagcattgaa agaaatggaa agctttgatc aagaaaaagt agaccatatc
     8221 gtgcatgaaa tggcaatggc agctttggat caacatatgc cattagcgaa gatggctgta
     8281 gaggaaacag gccgcggtgt ttacgaagat aaagcaatca aaaatatgta tgcgtccgaa
     8341 tatatttggc ataatatcaa acatgataaa acagttggtg tgatcaatga agatgtccaa
     8401 aaagggttga ttgaaattgc tgaaccagtc ggtgtggttt gcggagtaac tccaacaacg
     8461 aatccgactt ccacaacgat tttcaaatca atgatcgcgt tgaaaacaag aaatccaatc
     8521 gtttttgctt tccatccaag tgcccaaaaa tcttctgcag aagcagcacg agtggtacgt
     8581 gatgctgcta tcgctgccgg agctcctgaa aattgtatcc aatggatcga acatccttcg
     8641 atcgaagcga cttctatgct aatgaaccat ccaggtatcg cgatcgtctt agcgacaggt
     8701 ggagctggaa tggtcaagtc tgcttactct acaggaaaac cagctttagg tgtaggtcca
     8761 ggtaacgtac cagcttatat tgaaaaaaca gcaaaaatta aacgtgcagt gaacgatttg
     8821 atcgtttcga aaacatttga taatggaatg atttgtgctt ctgaacaagc tgtgatcgtg
     8881 gataaagaaa tctatgcagc agtaaaagca gaattccaag cacaccaagt ttatatcgta
     8941 aaaccagatg aattgcaaaa attagaagat gcagtgatga acgaaggaaa atatgcggtc
     9001 aatccatcaa tcgttggaca ttctgcgatg gaaatcgcaa aattggcagg aatcagtgta
     9061 ccaaaaggaa cgaaaatgct gattgctgaa ttggaaggcg taggcccaga ttatccgcta
     9121 tcaagagaaa aactttctcc agttcttgcc atgatcaaag ctaacaacac ggaccatgcg
     9181 tttgatttgt gtgaaggtat gttgaatctt ggaggattag gacatacagc agttatccat
     9241 tcagaaaacg aagagttgca tgtcaaattc ggtcttcgaa tgaaagcttg tcgtatttta
     9301 gtgaatacac catctgcaga aggcggcatc ggtgatatct ataacgaaat gattccgtca
     9361 ttgactttgg gatgcggttc ttatggtaag aactctgttt ctcgtaacgt ctctgctgta
     9421 aacttaatca atgtcaaaac tgtagcgaaa cggagaaata acatgcaatg gttcaaatta
     9481 ccgccaaaaa tcttctttga aaagaactct ctattgtact tagagaaaat ggaaaacgtt
     9541 gaacgtgtca tgatcgtttg tgaccctggt atggtccaat tcggttactg cgatacagtt
     9601 cgtgaagtat tggcacgacg taaaaatgat gtgaagatcg aagtcttctc agatgttgaa
     9661 ccgaacccat cgacaaatac cgtatacgca ggaacgaaac tgatggcaga cttcaagcca
     9721 gatacagtaa tcgctcttgg aggcggttct gcaatggacg cagctaaggg aatgtggatg
     9781 ttctatgaac acccagacac atctttcttt ggtgcaaaac aaaaattctt ggatatccgt
     9841 aaacggacat ataagatcga caaaccagaa aaaacacaat tcgtatgtat cccgacaaca
     9901 tcagggactg gttctgaagt aacgccattt gcggtcatca ctgacagtga aacacatgtg
     9961 aaatatccgt tagcagatta tgcattgacg ccagatgtcg caatcgtcga tcctcaattc
    10021 gtcatgtcag ttccagcatc tgtcacagca gataccggga tggatgtgtt aacacatgca
    10081 atcgaatcct atgtttcagt catggcttca gattatacac gtggattgag cctacaagca
    10141 atcaagttag tctttgacta tttagaaaaa tcagtaaaaa cacctgatat ggaatcacgt
    10201 gaaaaaatgc acaacgcttc tacaatggct ggtatggcgt ttgctaacgc attcctaggt
    10261 atttgtcact cagtcgcaca taaaatcggc ggggaatacg ggatccctca tggacggaca
    10321 aatgcgattc ttttaccgca tattatccgc tataatgcaa aagatccaca aaaacatgcg
    10381 atgttcccta aatatgacta tttccgtgca gatacagatt acgcagatat cgcaaaattc
    10441 ttaggtctga aaggcgaaac aacagaggaa ttagtcgaag ctttagcaac ggctgtttat
    10501 gaattaggaa aatctgtcgg catcaacatg agtctaaaag ctcaaggagt cacacaagaa
    10561 acactagata caaccgtaga ccgtatggct gaattagctt acgaagacca atgtacgaca
    10621 gctaatccga aagaaccatt gatctctgaa ttgaaacaga tcatcattga tgcttacaac
    10681 ggataagcaa aagaaagcgt agaaaaaaga taagaaaata gatgaaaaag aacttggaaa
    10741 acgttgtttc tccaagttct ttttttaatt tccaagtaac tgaaagttac ttttgttatt
    10801 taacatttag gtcaatttta gacagttttt gttcaaaaag cgatatttat agtggacggt
    10861 attactgcta tattaaggat ataaaatgaa agcgtttaaa aaggtggcgt aaagatgatt
    10921 attaaagaag agatttttgt acctaaagaa ttagtgaaag aaaaaattga cctgctcgtt
    10981 gaaaacctga caacgatcaa agacgataat ggtgaatttt tgttggactt tgatggactg
    11041 aaagtagatg acaaaagctg gacagtgtgg aattggccac aaggtgtcgg tctatatggt
    11101 atttataaaa attaccagat gacaaaaaat cctcgcgctt atcaagtggt caatgaatgg
    11161 tttgaggatc gtatggaaga aggcgctccg cccaaaaacg tcaatacaat ggcaccgcta
    11221 ttgactatgg cttacttgta tgaagacacg aaagattcac gctatttgcc ttatttagaa
    11281 caatgggcag aatgggtcat gaatgacatg cctcggacaa atgaagacgg gttacagcat
    11341 gccacatatg gaccggaaaa taagaatcag ttgtgggacg atacattgat gatgacagtg
    11401 cttcctttgg caaaaatcgg gaaactgttg aatcgcccag aatatttaga agaagctcgt
    11461 taccagttta tgatccatat caaatacttg atggataaac gaacaggatt gtggtatcac
    11521 ggctggacgt ttgaaggaaa tcataactat gccgaagcat tttgggctcg tggaaactgc
    11581 tggttgacga ttgctattcc agagatcatt gaaatcttag aattatcgaa agaagatgca
    11641 ttgcgcaaat tattgatcga aacacttgaa gcacaagtgc gtgcattaaa aacttatcag
    11701 tctgaaagcg gtttatggca tacgttgttg gatgatcctt cttcttacgt agaatcttct
    11761 gcaactgcag gatttgctta cggtattttg aaagcggtcc ataaacgtta tttaccacaa
    11821 gaatataaag aagtcgctta tcgtgctatc caaggattgc ttgaacaaat cgatgaaaaa
    11881 ggagaagttc aaaacgtttc aatcgggaca ggaatggggg acagtttaga tttttatcgg
    11941 aatatcggaa tcacagcaat gccttatgga cagtcgttaa cggtgctttg tttgacagaa
    12001 ttgctcgtct cttattgtta aaagaaagga aacccatatg aaaaaaacat cactgctgtt
    12061 tgcagcaagt tcgcttagcc taggtctgct aggtctaagc ggctgcggaa ataattctag
    12121 tgtggcaact gtcgcagatg cagatgtaaa tgagatagtt accttgcgct ttgcttatgc
    12181 aagcaacagc cagccagtaa ttgatgcaat gaatgaattt gggcgtttag taaaagaaaa
    12241 aacagatggt tccgtacaga tccagtattt cccagatgga caactgggtg gagaacgtga
    12301 actgatcgaa ttgacacagt caggagcagt agatttcaca aaagtaagtg cctcagcgtt
    12361 ggaaagtttc tctaaagatt attcgatttt ttcagttcct tatctattca atgatgaaga
    12421 acatttcttt aaggtgatgg ataatgaaga aataatggag ccaatctacc aatcgaccaa
    12481 aaatttagga tttaccggat tgacctatta tgattctggt cagcgcagct tttatatggt
    12541 agacggtccg atccatacgc cggaagattt ggagggaaag aagatccgtg tgatgcaaag
    12601 cgaaaccgct atcaaaatgg tagagctttt aggcggttcg cccgtaccta tgggaagtga
    12661 tgaagtgtac acgtctcttc aatccaattt gatcaacgga tcagaaaaca atgaatttgt
    12721 cttgtataca gctggacatg gtggtgtggc taaatactat tcctatgatg agcatacacg
    12781 tgtgccagat atcatcatca tgaatgatgc catcaaagaa cagctaactg atgagcagga
    12841 aaaagccatt gaagaagcag cgaaagaatc aacggttttt gaaattgaag cttttgcaaa
    12901 agcaattgaa gaagaaaaag ctttagcaca agaagaacat ggagtccaat tcaatgatgt
    12961 agataataca ccgtttcgag aggcagttgc cccacttcat gatatattca aaaacgatcc
    13021 tacctacaaa gacctttatc aaaagatcca agagcaagga gcgtagctgt gatgaaaatg
    13081 attttaaaca aggtattaga gatcctggga gctgttttgc ttgttgggat ggtcgctgtt
    13141 gttttatggc aagtattttc cagaacggtt ttaggaaatc caaatacggt cacggaagag
    13201 cttgttcgat tcggtcttgt ctggtttgcc atgttgtctt ccgcgtatgt tgtgggacaa
    13261 aaagcacatt tagccgtaac ggttttaagt gaaaaattaa ctggcaaaaa aagtaaaatt
    13321 ttagaagttg ttgttcaaag tttatttctg gtatttgctg cgatgatcat ggtgtatggc
    13381 ggatggaatg ctgtcacatt gacaatgggg cagatctcgc cttctttaag tattcctatg
    13441 ggatatgttt atctgtcagt acctgtatcg ggggtactca tcattatcta tagcctgatc
    13501 aatctaatcg atacattaag caacaagcca gcaattcaag aaaaaatata aaaatggagg
    13561 aataaaatgg caatagaagc cggattagtg ttattttttg tatttgttgt gttgcttgta
    13621 ttagggatgc cgattgctat aagtattgca ttctcatcga tgatgacttt attgttagtt
    13681 ataccgtttg atgtttctgc ttttagttca gctcaaaaaa tggtgggaag tatcaatagt
    13741 ttttcgctga tcgctattcc gtttttcgtt ctctcgggca ttatcatgaa taatggaggg
    13801 atagctaaga aactagtgga ttttgcgatg ttgttcgtcg ggcgcgtgcc gggggctttg
    13861 gctcatacga atgtgttagg gaatgcttta tttggttcca tctctagctc ggccattgcc
    13921 gcttctaccg cgattggcgg agtgttgatc cctcagcaag tggaagaagg atatgaccgg
    13981 aagtttgcga cagcggtcaa tatcgcgtca gctccgacag gtatggtcat tcctccaagt
    14041 actgctttta tcatgtattc acttgttgct ggtggtgctt cgatctctgc tttgtttatg
    14101 ggaggttatc tcgtaggcgc tttgtgggcg ctaggaatca tgctagtagc attcatcgtt
    14161 gctaaaaaga ataaataccc tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata
    14221 ataaaagagg cgattcctag ccttctattg attgtcatca ttatcggtgg tatcttgacg
    14281 gggatattta cagcagtaga agcttcagcg atagctgtca tttactccct gttgattgct
    14341 atgttttact ataaaacggt aaaactaaaa gatataccta atatgttgtt tcaatcagta
    14401 gcaatgtcag ggacgatcat gttcttattg gctacttctt ccatgatgtc tttcgccatg
    14461 gcgtttaccg gtattcctca ggcaattagt tcgttgatta tgggcgtaac ggataataaa
    14521 tttattattc tgttattagt caatcttgtg ttattactgg tgggtatgtt tatggatgtg
    14581 gcgcctgcta tcttgatttt tacaccaata tttttaccaa tcgtcacaag tgtaggggtt
    14641 gatcctgtac attacgggtt gttttcaatc atgaatctgt gtgtaggctc catcacgccg
    14701 ccagtaggta caggactata tgtcggtgca agtgtaggag gcgtaaaagc agaggcaatg
    14761 ctgaaaccgc ttcttccttt ttatggggtt atcttagtcg tcttgttttt gattacttac
    14821 ttcccgcaaa tcgtcatgtg gctacccaat atgctgagtt aggagaatag ttatgttgaa
    14881 cattacaatt agaggacatg atctttccga agtccaaacg atagaagatc ttgcagaaaa
    14941 aacaaaagaa caagggattc atacactgca gctcgcctta ggtctttcgt ttcctgaaac
    15001 accttctggc gcaaatgaga tcaattctgg gatgggaaat tatgtcaaac gagttttaga
    15061 aaagcaagaa gtgaccgtag gaattttaag ttgctatatc aatatgattc atccagattt
    15121 aacgatacga caagagttgc tgacaaagtt cgagcagtat cttcgctatg cttcttcctt
    15181 tggtgcgtcg atggttgctt cggaaactgg atgtgttttg ccggaaatcc aatatacgga
    15241 agaaaatttc acagatgaag catttgctga agctgtgtcc gttatccgtc gcttggtaaa
    15301 agctggagaa aaataccaaa tgatggtggg gatagaacca ggtttgaatc atccggtgta
    15361 ttctttagca agggtagagc agctgatcca agcagtagat tcggactatc taggaatcat
    15421 tttagatccg accaatttga tcacaagtag tgattatcaa gaacaagtcc aattggtaga
    15481 agaagctttt gaacgttttg gtgaaaagat atgtgcggtt catttgaaag attttcgtgt
    15541 cgaacaggaa aaaatcgtgc cggtaaattt gggagacggt gtgatcgagt acacaaaaat
    15601 caaagaaatc atcatgaaga atcgtccgta cctgtatgtg gttcttgaag aaacaaaaga
    15661 tgatgggatt cgttatggaa gaagtcttct tgaataatgt aaagaagacg ttggttaact
    15721 aatatttaga atggaggggg cggggctaga agatgccacg tctcttcttt tttataagag
    15781 tatataaaag cagctctttt tgattacaaa aaacgtggta taataaaatt gtgcataagt
    15841 gaacaaaagg aggtgcggca catgctccaa aagaaaatca gcggtgaaca agtcaatacg
    15901 atggtttctg atcagtatga aatctatcat gtcaaagatg tcgtcagcca aaataaaacg
    15961 atctatcatc atcatgattt ctatgagatc catgctacat taaagggaga agcgactttt
    16021 tatttgaacg gacgacaatt tacgatcaaa gcaggaagtg tgctgctcat ccattccaat
    16081 gatttgcatc gtatcgtccg ccaatcttca gatgtattcg agcgtgtcta tatctttgtc
    16141 accccttctt ttctagaaag tcgttccacg ccatggtcta atctatcggc ttgttttcaa
    16201 ccaatgggag agaagaggag tcgtgtattg caaatagaac aggaagtatt gaagcaaaaa
    16261 ttatcattta tcgaccatcc tcctgataaa aaaatttacg gtgaagacat aaaatatgaa
    16321 caagctctgg tagaatactt gatttttctc aatcaagttg tacaaaaaga agagagtgcg
    16381 caagaacaga atgctgtagt tcctaatgag cgtgtggaac aaatgttaca atatatctca
    16441 aaaaatttat ctcatccgct ttctttagag gaaatggaaa agcagttttt tgtcagcaaa
    16501 taccatatca cacgagaatt taaaaagtac actggattta cgtttcatca gtatgtaatg
    16561 aagaaaaagc tgatctatgc caagcacttg ttgcgagaat accgcagctc tagtgctgta
    16621 tatagtaaaa gtggtttttc ttcttatccg cattttctga aagcattcaa gagagagttc
    16681 ggcataacgc ctaaagaatt tttgaaacgt gatacacaga aacagttggt tcattatgaa
    16741 cattttgaag atgaacaatg agaatgatca ctaaaaaata accgcagtag ctgctgttgt
    16801 ttacatagaa gcagaaactg cggttatttt atttttatct atatatttca aaatattttg
    16861 aaaaaatgac gtacatatta tatgaatcag ctgaatttac gaaagtctct cggcgatagt
    16921 cctgtttttg ctttgaacag gcggctaaag taagcaaaat tctcaatccc tatttcttcg
    16981 gaaatcattc gaaccgtaaa ttctgtatac cgaagaaggt ctttagcgta agaaagtttt
    17041 ttgctgatca aataatcgat tggtggagaa ccgataaatt tggaaaaatc tttgctcagc
    17101 tgatatttat tcaataagaa ttgttgttct aattgatcaa gtgtaatcgt ttctttgaaa
    17161 tgttcgtcta agtattgctt cagtttgtaa acgtaggcgg gaatatcctc ttcttcgaaa
    17221 tctagctgat acttttgcaa aatcagttca ttcagcaact cgtggatagc tacagaggac
    17281 tggaaatcgg tacgggcatt cgtgccttgt tgcatgtgga tcaattctgt gatcaaatgg
    17341 tggatcttgt tttctgatgg atcccctgtt gaatggaagg tgttatggcc gtcttttgtg
    17401 aactcctgat aaaatgattg gacgttctga ccattcaaat ggatccaatc catttcccat
    17461 ggttcttcgc tgatcgtttg ataacgctga tattcttggc agtcaataaa aaataagtct
    17521 ccagctttta gctgttgctg attatcatta tatgtaagcg aacctttgcc agacaaagta
    17581 aactttatca agtaagaagg taaatgtgct cgttcagtat agtaagggat tggtgctttg
    17641 aagtaaccga tttcttggat ataaaaaaat aaattcctgc ttctttgtgt aggcgtcaag
    17701 atcactcttt ttgagtcttc cgaccatgag tggttggctt tttcccagta atccatgtaa
    17761 acactcccaa cttgtcaaga taatgcaatt atataccaaa atagtgtgaa gcaaaatata
    17821 agattctgta ttatgatagc attgttaata aatgataacg ctttattaat aaagtgctaa
    17881 tcaacaataa agtaaaagag gtggagaaat gaatccaaca tttgttgcca tcgatatcgg
    17941 tgcatcaagc ggacgactaa tgaaaagtac gagggatgca aaaggttctt tgatcttgga
    18001 agaagtacac cggttcaaaa acggctttcg gaacaaagac ggtcacgatc gctggaagat
    18061 cgattatctt atcaaagaga ttttgattgg atttgaaaaa ttaaagcaga cagggattga
    18121 tcgccttttt gcggggatcg atacatgggc agtcgattac tgtctgattg atgaacaagg
    18181 ggatcggata aatgatccga ttgcttatcg ggacaagcgt accaatggag cagtagaaca
    18241 gttttctgaa atctattctc tagataagtt gtatgagcag acagggattc agatccagcc
    18301 tttcaatacc ttgttccaat tgtttgctga agaaaaagaa aatttggcaa aagcacataa
    18361 attgttactg atacctgatt atttagggta tgtctttact ggtaacatgg tgacggagaa
    18421 aactaatgca tctactatgc agctgctgaa tgcagggaca aaagaatggg aaatagaact
    18481 tcttgaaaaa atcggtgtct cgaaggaatt atttccacca ttgacagagc cgggaacgat
    18541 actaggagat ctgcaaaaag aacaattttc aaaatatgat ttgccagatg tgacctttat
    18601 cacagtagca agtcatgata cggcgtcagc tgtattagga acgccaggtc aaggagaacg
    18661 ttggggttat atcagcagtg ggacatggtc gcttttaggg attgaaacaa agatcgccaa
    18721 tgtttctcgc aatgcttttt ctgaaaatta tacgaatgaa tggggtgccc ataacacggt
    18781 gcgttttttg aaaaacatca tggggatgtg gttgatccag gaagttgccc gaatgcaaga
    18841 gtacgagtac agttatgcgg aattggcaga gcttgctgca aaagaaaaac cgctgcagca
    18901 agtgatggaa atcaatgacc ctcgattttt gaatccccca aatatgatag aagagattca
    18961 gcaatactgt tttgaaacag agcagccagt ccctgtctca tcgggagcta tcgctcgttg
    19021 tgtgtatgat aatcttgctc tttgttacgc acaagagctg atcaaagtag aacacttaac
    19081 tggaacgaaa ttggaaaagc tgcacatcgt agggggcgga tcgaataatc gtctactgaa
    19141 tcaattgaca gcagattatg ccggaatcat tgtagaagca ggacctagtg aagccacagc
    19201 aatcgggaat gttgccatgc aaatgctcag tacaggtcag ttttcgaata tagaacaagc
    19261 aagaaatgta atcaaacagt cgtttccatg tgaaactttt tacccagatg aaaataagca
    19321 aaacacgatg gaatggtacg tgaataaaat caaaggagta atcgtatgaa cacgattgaa
    19381 agaaattacg aacaagcaaa agaaaaatat gcaactatcg gtgtagatac agatgctgta
    19441 ctagaaaaga tacaggacat caaaatttct atgcattgct ggcaaggaga tgatgtcaaa
    19501 ggtttcttga caccggatgg cgaattgacc ggtggaatca tggcgacagg gaattttcca
    19561 ggagccgcac gaacacctga agagcttaga caagatctgg agaaggctta ttcgttgata
    19621 ccgggcaaac acaaattgaa ccttcatgct atctatttgg atacagaaga atctgtcgat
    19681 ctaaatgaaa ttgagccgaa acattttgaa aaatgggtag agtgggcaaa aaaagaagaa
    19741 atcggtcttg acttcaatcc gactttcttt tcgcatccaa tgatgaaaga cggctttaca
    19801 ttggctcatc cagataaaga agttcgtgaa tactggatcg aacacgggaa aagaagccgc
    19861 aaaatcgcgg agtactttgg gaaagaaact gggcaaacaa gtatcaataa cttttgggta
    19921 cctgatggga tgaaagataa tccgattgat cgttttaccc cacgggaacg tttaatggaa
    19981 tcactcgatg aaatttttac agaagagtta gatgaacggt atgtacaaga tgcagtagaa
    20041 agcaaactat ttggtttagg cgctgaagct tatacagtcg gttcccatga attttatatg
    20101 ggttacgggt tgactcgtga caaacttgtt tgtctagatg ctggacattt tcatccaaca
    20161 gaagtgattt ccaataaatt atcttcatta gctttattca gcaaaggaat catgttgcat
    20221 gtttcccgtc cagtgcgttg ggattcagac catgtggttt tgatggatga tgaattgcag
    20281 gaaatcgcta aagaattagt ccgtaacgaa ttgttggaaa aaacaaatat cggtctggat
    20341 ttctttgatg caacgatcaa ccgtatcgct gcttgggtca tcggtacgag aaatacacaa
    20401 aaagcactgc tgaaagctat gctggaacca gttgagcgat tgaaagaaat ggagctggct
    20461 tttgacttta cttcaagaat ggcttatacg gaagaattga aagatttccc gtatgccgat
    20521 gtatggaatt atttctgtta caaaaatcaa gttccagttg gtttggattg gctggaagaa
    20581 gtccaggaat atcaaaaaga cgtgttggaa ttaagaaaat aggagtgtgg ctatattgaa
    20641 aaaaatagat attttacaag cgccttatgt gaaagaaatg atcgaaacga cgtcgaatct
    20701 ttatcgcttg ggatgggatg aaagaaatgg cgggaatatt tcttatttac taaaagaaga
    20761 agagatcaca ccgttcttgg atcctgaaaa agtgatccgc cgcatctcga tgatttttga
    20821 tgccagccgt ttagcaggat gctattttat tgtaactggt tcaggaagat attttaaaaa
    20881 tgtagcgagc gaacctgccg agaatcttgg aatcgttcga gttgctgaag atggcaaaac
    20941 actggaactt ttgtgggggc tggaaaacga atgccttcct acaagcgagt tgccaagtca
    21001 tttcatgagt catatcgccc gtttagaagt agacccagat aatcggatcg ttatgcattg
    21061 ccatgctagt catctattag cgatgagttt tacgcatgaa ttagacgaac gttcctttag
    21121 tcgtacgcta tggcaaatgt gtacggaatg cttagttgtt tttccagaag gaatttccat
    21181 cattccgtgg atggttccgg gaacaaatga gattggggaa gcaacagctg aaaaaatgaa
    21241 agaaactcga ttggttttat ggccacaaca tggaatctac gggtcaggaa aagacatgga
    21301 tgaagtcttt ggtttgatcg agacagcaga aaaggcagca gaagtctata cgtacgtcaa
    21361 agcgcaagga ccaatcttgc aaacaatcac ggacgaaaac ctgtggcatt tagcagatgc
    21421 gtttggtgtc actccaaaag caggttattt ggaggaagtt catacgaaag caggtgtctg
    21481 acatggtgaa aaaagctttt tgcatgaaag tttacccaga aaaacaagaa gaatacaaaa
    21541 aaagacatga tgaactttgg ccagaaatgc gtacagcact tcgtgaacac ggggtcatcg
    21601 actattcgat ctatcttgat ccgagtacaa gtttgttatt tggttactta gtgatcgaag
    21661 atgaaaagtt atgggaaaaa atggcaaata catctatcaa tcaaaaatgg tggaagtaca
    21721 tggaagatat catggagaca aatccagatg cctctccagt cagttgggaa ttgatgccag
    21781 tctttgaact gtagcaacaa gcaactgggc aaccggttgc ttggggttct ttcgttttta
    21841 ttgtgaaaaa taatacaaat taggaggaat agaaaatgag taatcgaatg attttaaatg
    21901 aaacatcgta ttttggaaaa ggagcgattg agtcgatcgt ccctgaattt cagcagcgag
    21961 gattcggcaa agcagctgtc attactgaca aaggattgat cgagcacggt atagcaacaa
    22021 aagtaacaga acttctagat aaagcagcaa tcgcttatgc tttatacgac gggatcgtac
    22081 ctaatccaac gatccaaaat gtaaaagatg gagtggcttt tgttaaagag gcagaggcag
    22141 actgtttgat cgcaatcggt ggaggttcgc caatcgatac ggcaaaagcc attgggatta
    22201 ttttgacgaa tccagagttt tcagacgtca ttagtttaga aggtgttgct gatacgaaaa
    22261 atccatgttt acctatatta gctataccaa cgacttctgg aacagcagca gaagtaacga
    22321 tcaactacgt catcacagat gaagtaaatc accgaaaatt tgtttgtgtt gatcctcatg
    22381 atatcccaat cgtcgcgttc atcgacagcg atatgatgat gggaatgccg aaaaaattgg
    22441 ctgcatcaac aggtatggat gctatgacgc atgctattga agggtttatc acaaaaggcg
    22501 cttgggaaat gactgatatg ctgcatttaa aagcaatcga aatcatcggc cattctttgg
    22561 aagtttctgt agatggcgat caaaatgggc gagaaaaaat ggcattagga cagtatattg
    22621 cgggaatggg cttctcaaat gtcggtttag gactggtaca cgggatggct cacccgttaa
    22681 gtgcatggta caacattcct catggcgtag cgtgtgcagc acttttgcca actgtcatga
    22741 aatataacaa agaatatact ggagaaaagt accgtgagat cgctcttgtt ctagggatta
    22801 aaggagcagc tgaaatgtct ttagaagatg taagagaagc ggcttgtggt gagatcgatc
    22861 ggttgagcaa agcggttgga atcccagaaa ccatttctga attaggtgta aaagaagcag
    22921 atattccagc aattgcagaa gatgctttgc gagatgtatg cacaccagga aatccaagag
    22981 aaacgacagt agaagaaatc attgctttat atcaaagttt gatgtaaaaa cgaagcggat
    23041 cttcaacatt aaattgcctg taaagagaaa tggtagttta tatctggata tctcttttaa
    23101 attgatggta tttccgctat aatttttctt ataaagaaat aggagaatat tatatggaaa
    23161 ataaaaagcc ttatggaact gtattgatca aagcctctaa aatactagac tatttagcgg
    23221 aaaaccaaga tgtctctttg cagacgatcg caaagggtgt gggaatgacc tcttctacag
    23281 tcttgaaaat actagatact ttgttgatga tcggctacgt aaataaaaat tcagagaaaa
    23341 actatcgttt aggtgcaaag cttattcgct acgcgaataa aaacattgaa caaatcgatt
    23401 tggtagagtt gactttaccg tttttagaac agctgcagca aaagatcgat gagactatcc
    23461 acttaggggt attggacaac aatgagatct tgtacgtgaa caaactagat ccaaaacatc
    23521 aaacgatccg catgtcttca aaaatcggaa tcacccgacc attatacaat tctgcgatgg
    23581 gcaaagccgt attagcagaa ttcagtgaag agcagtacac tgattatctt gaaaagcatc
    23641 cgttgatccc ttacacagaa tatacgatca ccaatgcatt acggttgaaa aaggaaatcc
    23701 aaacggtaaa agaaacccac atcgcatttg acgatgaaga aatcgaacgt gatatttttt
    23761 gtataggagc ctcaatcgtg aaaaacgaac aaatcattgg tgcatttagt gtaagtatgc
    23821 cgaaatatcg tttaacagat gagacaaaag aacaaataat cagtgccctc aagcaaacaa
    23881 aaagcgaaat cgagcaaaga attcaaaaat aatgaaaaaa gcgtttgcaa tttttctttt
    23941 ctgccgtata atatgggtat agatttccaa gttataaaat aaaatttcta aataggaaat
    24001 aggtggagaa gatgaaaaaa attgatattt tatctcgatt gaaaaacgcc ggagtgattg
    24061 cggtggtacg cggaaaaagc aaagaagaag ctttgaatgc ttgccacgca atcatcaaag
    24121 gtggtctaac tggtattgag ttgactttta cagtccctca agcagatcaa gtaatcaaag
    24181 aacttttgtc attctacaaa gaccaaccag aaatagtgat tggcgcaggc acagtattag
    24241 acgctgtcac agctcgcttg gctatcttag ctggcgcaga gtatattgtt agcccttcct
    24301 ttgatcaaga aacggcagag atgtgcaatc tgtatcaaat tccttactta ccaggatgta
    24361 tgactattac cgagatcaaa acagcgctga aaagcggggt agatatcgtc aaactatttc
    24421 ctggaagcgc ttatggacca agtatcattt ctgccttcaa ggcgccgatg cctcaagtca
    24481 atatcatgcc aactggcggt gtgagtctgg acaatatgaa agagtggttc gatgctggtg
    24541 tcgtaacagt cggagtaggt gggaaccttt tagcacccgc agctacagga gattttgaca
    24601 aagtaacgga agtcgcgcaa cagtatgcag caaaaatgaa agaaatcaag aggtagctgc
    24661 ttatgggaaa agtagtgaca ctcggagaaa tcatgttgcg attgtccaca gagcagggaa
    24721 cccgtatcga ttcaagtgaa cgattcgctg cccattatgg cggaggggaa gcaaacgtag
    24781 caatttcttt agctaattac ggacatgaag cgatttttgc tacaaaagta cctgataacg
    24841 cattgggaaa agcagtcaaa aagcacttgc aatgttatgg agtagatacg aaacaggtat
    24901 tatctggtgg atcaagacta ggtacttatt atatggaaac tggtagtggc gaacgcgccg
    24961 cctcagtgat ctatgatcgt gccggatcaa gttttgctga aatggatcat ttggaatggt
    25021 cgctagagga gttattccaa ggaatagata tatttcatgt ttcaggaatc accccagctt
    25081 tgtctaaaaa atggcaaaaa atgacgaaac aggtgataga agctgcaaaa aaagctggct
    25141 gcctgatcag cttcgatatc aactatcgtg gaaaattatg gacacagcaa gaagccagcg
    25201 aagtaatcca tcagctttta cctttagtag atatctgctc agctggaaaa atggatgctc
    25261 tttatttgtt aggtatcgag aaagcgccag aagcagaaga acaacctcta atttattatt
    25321 atcaaaaaat gcaggaaaag ttcccgaata ttcaggtgtt ttattcgacc aagcgtaccg
    25381 ttcattcagc aagtgaaaat gaattgaagg ggacattgtg gatgaaccat gaatatgtgg
    25441 aatcccaagt ccatagaatc accccaatcg tggatcgagt aggtggcgga gatgcttttg
    25501 caggtggcgt gctccatggg attttatcca atatgtctcc gcaagaaatc attgattttg
    25561 ctacggcagc ttctgccttg aagcatacga tccatgggga ctgcaaccaa tttagtcaag
    25621 aagaagtcgc acaattttta gcatgcggct ctggaaaaat cattcgatag aaggagtaga
    25681 agaaaatgca agaaatggaa acacgttata cacacagtcc agaagatatc cgtcattatt
    25741 caacggaaca attgagaaaa gaatttttag tagaaaaggt atttgaacca gggaaaatca
    25801 gtttgactta tacgcacaac gatcgaatga tctttggtgg agtgacacct actgaaaaag
    25861 aattagaaat cacattagac aaagaactag gtgttgatta cttcttagag cgtcgtgaac
    25921 taggtgtgat caatatcggt ggaccaggat tcatcgagat cgatgggcaa aaagaagaaa
    25981 tgaaaaaaca agacggatac tatatcggaa aagaaacacg acatgttgta ttttcttctc
    26041 aagatgctga aaacccagct aaattctata ttagttctgt tccagctcat cataaatatc
    26101 caaatgtaaa aatcagtatc gaccaagtaa aaccaatgga gactggtgaa ggcctgacac
    26161 tgaatgaacg taaaatctac caatacatcc atccaaatgt atgcgaaagc tgtcaattac
    26221 aaatgggtta tacgatttta gaaccaggaa gcgcttggaa tacgatgcct tgtcatacac
    26281 atgaacgtcg tatggaagct tatgtctact ttgattatgc caatgaagat acacgtgtgt
    26341 tccacatgat gggcaaacca gatgaaacaa aacatttagt tgtagacaat gaacaagcca
    26401 ttatttcgcc aagctggtcg atccattcag gtgtaggtac aagcaattac tcattcatct
    26461 gggcaatgtg cggagaaaat atcacctata cagacatgga tatggtgcaa atggatcaat
    26521 taaaataagc ataagggaga gaaaatgatg gaatttagta tggatatgtt tcgtttagat
    26581 ggaaaagtag cattagtcac cggtgctgtc tatggtattg gatttgagat tgcacgctct
    26641 ttagctacag caggagcaaa aatcgtattt aataacttga atcaagaatc agttgatgaa
    26701 ggtatagcgc attataaaga agcaggcatc gatgcaaaag gttatgtctg cgatgtgacc
    26761 gatgaagaag cggttcaagc gatggttcaa cagatcaaag aagatgtagg ttcagttgat
    26821 attctagtca acaatgcagg gatcatcaaa cggacaccga tgatcgaaat ggatgcggca
    26881 gacttccgcc aagtaatcga cgtggattta aatgccccat tcatcgtttc aaaagcagtt
    26941 attccggata tgatcgaaaa aggcggcggt aaaatcatca atatctgctc catgatgagt
    27001 gaattaggac gcgaaacggt cagtgcttat gctgcggcaa aaggcggatt aaaaatgctg
    27061 acgaaaaaca tcgcttctga atacggtcaa tacaatatcc aatgtaatgg aattggacca
    27121 ggttatattg ctacaccgca aactgctcca ttacgcgaaa cacaagaaaa cggtgaacgt
    27181 catccattcg atcaatttat cgttggacgt acgccagcag cacgctgggg cgagccaatc
    27241 gatttggctg gaccatctgt cttcttggct tctagcgcat ctgatttcgt taacggccat
    27301 gtcttgtatg tggacggagg catacttgcc tatatcggaa aacaacctta aaagagcttt
    27361 agtacactgt ttagtggaga ggttgtgaaa tcagcggatt gaacgaatac cgaaggacag
    27421 ctgattaaac atcatttatt cgaaaaaaga ggctgggaca gaagcattta actccaagaa
    27481 ataagaagga attcacgaaa attgcttttc aaatttttgt gaatttcagc ttatttccga
    27541 aggagttgct tttaggctct tcgtcaatga gtgttggtga agaaattcaa tagaataatc
    27601 gagatactca cacggctaga cagctatctt gtctagccgt gttgttgttc tggctttagt
    27661 cagttgggta gtgttcttag ttttcttgaa atattttcct cgatataaaa agattcgttc
    27721 tcggtagttt tgaaagtttc taaagccata ggcaattcgt tttagtactt tgatatggtt
    27781 gttcaaacat tctatagggc cgttggaata gggaactcgc aacgctcgtt tgatttctgt
    27841 tcgatatttc ttgaaggtcg tgaacactgg ttgatagtgc tctggcaact ccttgtagtc
    27901 ttcttttagg agcgcgacga attcaggaac atcttgtctt ctgatagcgg aaagaaaaac
    27961 ctggtagact tcgtagcctc gctttaagga gtcatcataa tataatagcc gatcgactat
    28021 ctccgcttct gtcagatgat ctcgaaatga cggacgccag tagcgatgct gaccattcaa
    28081 ttcccacgca ttcttttgaa gtagtctcca gtatttcttt aagtgcttcg accgacgatc
    28141 ccctttttta aaggtcttca ttacttgaat tcggtggttg gtaaaggccc gaccgatgtg
    28201 ttggctaata tgaaagcgat cggcgacgat tcgggcattc ggaaaacact ctttcaccaa
    28261 tgtgcgatag ggtgtataga aatctgaaac cactagacgt acttgttcac ggacttttct
    28321 agggtagcgc aagaaatagt cgcgtaaagg attgagttgc cgattttcca caatatcaat
    28381 cagttgatgg gtcttgccgt ccatcatgac aaagctcatg gctcccgaga cgtttttcac
    28441 cgatttgaat tcatcgaaac acaccacttc tggtaacggc tggttcaaag aaacggtttt
    28501 aggccgaagg ctgcgaagta ttcgaatgac agtggtcgga gaaatgtgct tcatacgtgc
    28561 aatcaaagac atagaaatag gctcacttaa tagctcaaga atggcttgtt tgactctgcg
    28621 ggcaatggag cagcgacgat cgctaactga atcttctgct aaaaaagtct ttccacagtg
    28681 tttacatttg aagcgctgtt tgttaatccg cacaatggtc ttgtagttac tgacatcatt
    28741 caataagatg gatacttttt tccagcccca tttaatgatc gtttgcttat tttgatagtg
    28801 gcaatggaag cattcctctg gttgataggt caatgtgcca tcaaaaacaa aacatgtttc
    28861 gtcattaatg cgctctttgc gaaaggcatc ttcatgaaag gtaagattta ggtctagaat
    28921 atctagtaac tctttggtaa actctgaata ggacatcttg aaacactcct ttgaatggtt
    28981 ttcgtcgact ttattctaaa ggatttcaag gtgtctgttt gcgtttatta gaaaaaagaa
    29041 aaagtattgg caacagaagg cgtcaccaac actagatatt atagatcctg cttttacttc
    29101 cgccgtttgt acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc
    29161 ttttctgtgc gtcttccata cttcactaag tcgatccctc ttgcttcgca ccaatccttc
    29221 actggtccgg aaaagatcag agattgttgg tgtagggccg cggtattcgt tgcgccgacc
    29281 atagtataaa gcagacgaag ctcttcttgc cattgcttca tcagtataat agtttcttct
    29341 acgccgtgat tcatcagatg cgtcagtacg gttcctgaag tacccacggc atttgcgccg
    29401 agacaaaggg ctttaaatat gtcataggca ttgcggatac ctccggaggc taggatttcc
    29461 atagaagtgt ctgcttcatt tgcttcaagc aatgaagaga ctgtcgattg cccccagtca
    29521 gctaaataac tcagttcccg cttggagcga cgggcgtttt cgatctgggt gaagcttgtt
    29581 ccgcttcgac cgctgatatc aactgtatga actcctaaag cagctagatc attgagcgtt
    29641 tctcttgtca tgccaaaacc gacttctttt acgatgaggg ggacgtcaat ggctgtttga
    29701 gtttcttgaa taagtacttt ccaattggta aaatcacggt ctccctcagg cataaccaac
    29761 tcttgaggtg cattcaaatg aatctgtaag gcatctgcgt gaaacaatcg gatagcttct
    29821 ttggctcttt cgacagaagt ccctgcaccg atgttggcaa tgattttccc gtgaggatat
    29881 tcttgtcgca taatcgtata agtatcggct aaagaaggga ctttcaaggc agcgctgact
    29941 gaaccagtgg caatcatcaa atctgcttct cttgcgacga ttgcaagatc ttggttgatt
    30001 ttttttgttt tttcgcttcc gccagtcatc gcattgatat aaaatgggct gcttagtgta
    30061 aacccaactg cctgtgtact tagatcgaca tcagaaactg ctatttgcgg caaagggtta
    30121 tggatgatcc gtacgaagtc aaattcgttt ttttgtttat catgaaaggc tttagctagt
    30181 gaaacatgtt cgtcttttcg attcatcggt tttcctttct ttgatcgcta tagacatgta
    30241 acggcagtgg agtgatttct gctttttccc atgcactcat taaaggaaga atgccagatt
    30301 tctggtcaac aattacgatt ccgcaatcgc ccccacctgc accagaagat tttgcggctc
    30361 cttcatactg ttcagctaaa ttacacaatt tgttcaaagc aggcgtttcg atgacgaccc
    30421 cagtgattgc agataaatca tgcagtaatt gtcggttttt tcgaatcatc tgttggatca
    30481 acgttacatt attttcttta aaccctttga tcatttcgtt gacacattct gtactatttt
    30541 ttaagaaaag ctgataagcc accattttat cttctctcga acggtgaact tgatcgacca
    30601 aatcagaagt agaggcaggg ctacccgtcc aaccaatcaa taaacgtaaa tcttcaggag
    30661 caatcaatgg ctcaatggat agacctggcc aatccagggc aagtaactca ctgatagaat
    30721 gctgagtttc ttgttcttgg agccaaggat ggtcgaaggt tgagaaagcg atccagccac
    30781 cataacagct agcagcgatg tcgccgcagg aaccattatc ttgaactgct aaattggcta
    30841 gtgctgcaat cttgaaaatc tccaactgag ataaattcaa cgcgtaaaaa acattcaagg
    30901 ctttgaccgt tgcaacggtc acggcaccgc tagaacccaa tccatatttc cggccatttg
    30961 aactgtctaa ctcacttgtt actttcagat cataaaagga taaaagaatg tttttttctt
    31021 gtgcatactt ttcagtcaag cgaatagcag caaggatata atgaaaagga ttttcccgaa
    31081 tatctaaaac taattctccg ttgcgtcttg tccaacgtac aggcatccca ctatattgtg
    31141 cagattggat acttccgact tttcgtgcag attctacagt gactgtcacg aattgatcga
    31201 ctgcagcgat aactgctgga tggcctgttt caacaactgc atattctccg gcaatataga
    31261 gtttgcctgg tgcagatact tcaatcataa aaatctccct ttattcaata atcgcaattc
    31321 ctggaccagc atgtgcagta attacttgtt ctttgctaaa taatttggta aatgtttctt
    31381 gtacctcaga taaatggctg ttttccacta aaactttgac attagggcca gcatccatcg
    31441 taaagtagca tggaatgcct tgctttctca attgtcgcac cgcatccatc gcttttaagc
    31501 tgtcaggaga ccagtaagtg aatggaggct gggcagctaa agtcgttcca tgcattttta
    31561 gtccgtttct ttccatcgtt tctccgagaa gttggaaatc ttttgttttg attgcttgtt
    31621 tcaattgata tagatcccct tcaacggaat ctaaccagcc ttgataaaaa ctggatgttt
    31681 cgactgtccg acgcatccca tttctgctgg acacttcttt tttctggtcg ttgatcaaaa
    31741 cgaaaaccat tgctaaatcg tcttcgaaag aatcggaagg gactggctta gcgtaagaac
    31801 ttaagtcgtc atggcctttt tcccattcga cgaaaccacc gaaaatactt cggcaagctg
    31861 aaccagaccc gcgtcgagca aatcttgaaa gagagagatc atctaatcca agcttaagag
    31921 cagtattgca agcgcctgct aaagcagcta gcccgctggc agaggaggct aatccagctg
    31981 cggtaggaac gaaattctga ctgattactg aagcttttag tgagaggccg gcttctttac
    32041 gaaaaaggtc aagaaactga ctgacttttt ttgttgcttt ttcgtcttgc aaggtgccat
    32101 ctagataaaa ttcatctacc atatagctat cagaaaagat gacttctgtc tctgtataaa
    32161 atgcatccaa tgttaacgaa aggctgttgt tcattggaag gatgagttct tcatttttct
    32221 taccccaata tttgattaga gcaatgttgg tatatgcgcg tgctttgcct ttaaacatag
    32281 gtatgtactc ctaatccttg tatccaagtt tcagcagcac ccgcatcttc aagtgcttga
    32341 ctgatttctt gggcttttgt ttttgtttgt gctaatgcaa tcatacagcc accgcgtcca
    32401 ccgccagtta atttggcacc caatgccccg gtatcttttg ctgtttggat caatagatta
    32461 agaaaatcat tgctgattgt cagcttttcc agaagtgatt gtgattcatc cattgtctgt
    32521 gccaacgttt ctggtgaatt ttcaatgatt gcttgttttg cttgcttcgt caagtatcct
    32581 aatttttgga tcatttgtcc agtttcatga ggctgggttt caaagaggtg agcgacatct
    32641 ttgacggctt ctcttgtttg tcctttgatt cctgtatcag cgacaatcaa aaaagcatcg
    32701 atgttcaaag aaaagtagtc gaaaggatgc ccttttgtaa aataaatcgg ctgcaagctg
    32761 ctagtagcgg ctgcatcgat tccactagga ttaccgtggg cgattttttc cgaaagctgg
    32821 acattttcta atagtatttc acgagacaaa ggaaatgcta agtaatcata aaaagcacga
    32881 gtgactgctg tggcgactgc agcgcttgat cccattccac gttcagcagg aatcgtgctt
    32941 tcaatggtca gttgcaagtt tttttggatg tcatgctgtt tttttaagtg aatgaataat
    33001 tttttgatat tttttaatgc atgaggagct tcggctaaat ttcctgaata gtaggaagaa
    33061 accagttgat cgtccattgc atccagctct tcaaggaatg cggtgacttt tgttgcatag
    33121 aaaggaaagg caatcgctgg ttctccataa acaaccgcgt gctcgcccat caatatgatc
    33181 tttccgcttg attctccttg gccatagttt gccataatct aactccttct cacgtaaaag
    33241 tgatttttaa gcaaaaaata aagcgcccaa aacgagttgc gctgcttcac gaattttttt
    33301 gtccactaag ggtctcaaaa aacattcctc ctttatttta ctaaaaccat ctcagaagta
    33361 aaactaagaa tacgttttct aaagaaaaaa cacttttttc ttaagaattt tttgactttt
    33421 tttcttcttt gactagaaaa aaatacaatt gacactacta gatattgggg gtatatttaa
    33481 aaaggttgga gttaaacaca atatttagtg atgaagtgag ctggaggtaa atgagcgtga
    33541 tcgttttagc aggtacaatt ggtgccggaa aatcaagttt aacagaaatg atggctgaac
    33601 actttgatag tcaggcattt tatgaatcga ttgatgataa tgaagtcttg cccttatttt
    33661 atgcaaaccc tgaacagtat gcttttttgc tgcaaattta ttttttgaat aaacgttttg
    33721 caagcatcaa acaagcaatg aaagatgaca ataatgtatt ggaccgttcg atttatgaag
    33781 attcactgct tttccatcta aatgcagatc taggaagagc aacagaaaca gaagttcgtg
    33841 tgtatgatga actgctggaa aacatgatgg aagaacttcc ctatgctgcc cataaaaaac
    33901 atccggattt attagttcat attcgtgtat cttttgatac gatgttggag cgtatcgaaa
    33961 aacgtggtcg ttcgtatgaa caattgtcct ttgatccaag tctatacgat tattacaaag
    34021 aattgaacag acgttacgat caatggtatg aagaatacaa agaaagtcca aaaatccaaa
    34081 tcgatggaga ccgctataat tttgtggaag atcctcaagc aaaagaagaa gtcttgaaaa
    34141 tgatcgaaga aaaattagca gagattcgcc aaaccaaagt agccatttga tttacagttg
    34201 ctcaaataaa gggtaaacct ttatttgagc gacttttttc tttatactgg aaacaaatgt
    34261 caggaggata gagtatgcgg gaaaaacact attttaatca agaccgtaat ttttggatca
    34321 atgtacagca aaacgatgtg ctagctgtta aaaacttaaa agaagaatat ggcattagtg
    34381 acgagatgct gacttattct ctagataaaa atgaacgggc gcgcgtagag tacgattctt
    34441 ttgatgaagc gttactgctt gtatcaaatg ttccgcacca gcaaaaagtc gataatcatt
    34501 atgaaactag tcctatcgct tttattttaa aagaagacgg tttgtttaca tttaccacgc
    34561 cgaataccga gtacgtgatt cgactaattc gatcgctttt ggaacgaatg ccagacatga
    34621 gtgtatacag tttattgttt cgtacattgt ttttgatttc tgattccttt tttcctttaa
    34681 tcgaggaagt gaatagtgaa cgtcagcggc ttaatctgaa attaagagaa aaaacaacga
    34741 acaagaatct gctgcaactt tcagatttag agatcggttt ggtttactta gttacaggaa
    34801 caaaacaaaa tgtagtactt ttagaacaaa tcaaggcatt ggctatttat cgcaagttgt
    34861 cagaaaagga aaaagaagag ttggacgatg cgctgatcga agcacgacaa gcagttgaaa
    34921 tgacgaactt agcttcgcag attttagacc agctctctgg cacttataat aatttgctga
    34981 ataacaactt gaatgacacg atgaaatttt tgacggtctg gtcgctgatc ttgaccgttc
    35041 caactattgt gacaggattc ttcggaatga atctccagtt accctttacc cattccgttt
    35101 tcggatgggg aatcgccttg atcatcagtt tggtcttatc tatttggatg cttattgcac
    35161 tgtggcggcg gattcgttga tctagtcttt gatctgcaaa ccaagggctt gggcgatcac
    35221 aagtgcttgc tcttggttga cgattagtcc cgccaaagaa tcttgagata aagcgattgc
    35281 tgtgaagaaa ttagtcgtaa agtctaatcc cttcagagaa gttcgaaacc agttcgaacc
    35341 ggtcaactga ttgtcagaca aaaaaaggtt tttccaagtg acctcataaa attcgctgtc
    35401 ttctaattga caatgatcga aggagacagc tttcaagttt gtactactaa aggaagccat
    35461 cgaagcaata caatcagtga aagtacagtc tcgaagataa ctttcggcaa aattcgtacc
    35521 tgttaattta cattgatgaa acacgacttg gtggaaactg ccaccaatcc actccagatt
    35581 ggaaaaatca catttttcaa aaatgacatt actgcattca aaatgacgta aaactgtttt
    35641 ttgcatcgta agtttttgga tatgactctc tcttaatacc atatttttgg catcgagata
    35701 gctttggtca cccccgttga tcagaagacc agagaagatc tcctcatctt ctaaataaaa
    35761 ctctcccttt gtaagaagag gaagagaagg tggaataggt tcgatttttt tcatgtgggt
    35821 tcacctcgtt tttctttagt atagcaaaga agcgggaatc actgctagaa agtctgctaa
    35881 tctaatcatt ttcagaaaag gaagacaaac tgtatgaaaa acattgacaa aaagattagt
    35941 ttttgctata ctatgatggt tgagaaataa aagcagaagc acccgcttct cgccttagtt
    36001 gataatgtca ctgggctaga tagttttcac tggttctatt aggtgaagta cgcgcgggtt
    36061 ctgtaagaga actcgttttt ttattgccgt ttattcggta agcttttctt tctctcataa
    36121 tatttggagg tgaatgacca tagcaaagga tatgatggtt aacgacggca ttcgtgcacg
    36181 agagttacga ttgatcggtt cagatggtga gcaattagga gttaagacaa aagcagaagc
    36241 attgaatatt gcagaacaag caaacttgga tcttgtgtta gttgcccctg gtgcgaaacc
    36301 accagttgcg cgaatcatgg actacggtaa ataccgtttc gaacaacaaa agaaagaccg
    36361 cgaagcgcgt aaaaaacaaa aagtgatcaa tgtaaaagaa gttcgcttaa gtcctacgat
    36421 cgacttgaat gacttcaata caaaacttcg taatgcacgt aagttcttgg aaaagggcga
    36481 taaagtgaaa gcttctatcc gtttcaaagg ccgtgccatt acccataaag aaattggtca
    36541 aaaagtcttg aatcgcttag ctgaagaaac tgcggatatt gcaacagtgg aacaaaaagc
    36601 gaaaatggac ggacgcagca tgttcttgac gctggcaccg aaaaacgaca gtaagtaatt
    36661 tgaatgacta actggctgtt ggacagccga tagtcggata acgaaagatt ttgaggagga
    36721 aatatagtca tgccaaaaca aaaaacacac cgcggatcag caaaacgttt caaacgtact
    36781 ggtaaaggcg gattgaaacg cttccgcgcg tttacaagtc accgtttcca cggcaaaaca
    36841 aaaaaacaac gccgtcaatt gcgtaaagca agcatggttt ctagtggcga cttcaaacgt
    36901 attcgccaac aactagcaaa aatgaaataa gaagtacaaa taacagaatc tgaagattta
    36961 tttttattag gaggaattat acatggcacg tgttaaaggt ggaacagtaa ctcgtaaacg
    37021 tcgtaaaaga gtgctaaaat tagcaaaagg ttactacggt tcaaaacaca ctttatttaa
    37081 aacagcaaaa gaacaagtaa tgaattcata caactacgca tatcgcgatc gtcgtcaaaa
    37141 gaaacgcgac ttccgtaaat tgtggattgc tcgtatcaac gcagcagctc gtatgaatgg
    37201 cttgagctac tcaaaattaa tgcacggttt gaaagttgct gaaatcgaca tcaaccgtaa
    37261 aatgttagct gacttggctg ttaacgatgc agcagcattt actgcattgg ctgaccaagc
    37321 aaaagaagct ttggctaaat aagctaaaca attataaaga ggaaagaaga taacgaaaag
    37381 ttatgttctt tcctcttttt ttatttacaa tcctccatca aatgaaacag gacaataacg
    37441 tataagtcta aaatagcata attaaggttt tttaattgaa aactgaacat ttatggtcac
    37501 cctttaatga aagcgctaac tacataagtt agaatgagtt tgactgatat attagtttgc
    37561 ttatcagaaa aacaaaatgg acggaggaat aacatgagtg atattattat tggtattttg
    37621 ttagttatct cattcttttt catggtctgg tactgtgtaa aaggctacaa cttgatggtc
    37681 ggctttgcca tcatggctac tgtatggatg ggattagcgc tagtcggaaa cacgttttca
    37741 ccaaacccgg cgatggaagg gcaaggagta attgatgtgt tgacgcatat ctatacgacg
    37801 gggccagctg agtacgccaa atcgatcttg gtaaatgtct tctttggtgc ctttttcggg
    37861 cgtgttctag tagatagcgg aatcgcagct accctgattc gaaaggtagt tgaacttgga
    37921 ggggacaagc caagaatcac gatgagctta ctatgtattg taaccgctgt tatattcatg
    37981 tcgatgacag gaatcggacc agtgatttct attgcagtta ttgtgttgcc aatcttgatg
    38041 tcattaggta tttcagtacc ggttgcactg ttctcattta tgggctcgat tatggctgga
    38101 atctttgcta atatcgtcaa tttcaaacaa tatcaaacga tttatgctgg tttcaatcca
    38161 gcggctgaaa gctatactta taatgactat ttccaaattg gtatgattgg gatgattgta
    38221 agcttagttg tggtactaac ggtcgctaat atatcaatga acaaaaagaa acgctatgca
    38281 atggcagcaa acgtaccagc tgaaggtggc gacgcaccaa tgatctcatg gttagccgta
    38341 ttattaccag ttttaggggt cgttctttta gatcttccaa tcatcttagg ttttattcta
    38401 gcaggtatct gggcattgct gtttactgga aaattgcgcg gaggttataa agaaatctgt
    38461 cgtcagtttg cgaaattatt tacagatggc gcagtagatg ttgcaccaat ggttggtttc
    38521 ttgatgacct tagcgatgtt caataattca gctgcgtatg cttctcctta tttttcagct
    38581 atttttggag attggatacc gcaatcacca ttagtcttgg caatcgtatt tgctatattg
    38641 acaccactag gatttttccg cggtccaatg aatctagtcg gttcaggctc agctatttta
    38701 gctgtcgtat tagctgttaa tccaacgatg tcgccagcct ttttgttccc gttatttgca
    38761 atcacaacga ttgctcctca gcatttagat attacacaat cttgggttgc ttggggatta
    38821 ggttatacaa aagtaacttc tcgagaatat atgaagaaat ccatcccaac tggttggatc
    38881 atcggagcga tcttatgcct gatcacgttc ttattgtacg ggaacgccta gtaaacaaaa
    38941 cgtcgataaa aacgattaga tgaatggagg aaaagaaaat gaaacgtttt gtgcgaatgg
    39001 gaattgatgt tggcggtaca catacgaaag cagtcgcaat cgataatgcg acgcacgaaa
    39061 tcatcggaaa atcttcagtg aaaacaaccc acgatgatgt ccgtggagta gccgcaggtg
    39121 tcgttcagtc ttttcaaaat tgtttgcggg aaaataatat cagtccagaa gatgttgtgt
    39181 ttgttgcaca ttctaccaca caagcgacta atgcactgat tgaaggagac gtagcaaaag
    39241 tcggcgtaat cgggatggca aaaggtggat tagaaggttt tttagcaaaa cgacaaacta
    39301 gattgaacga tatcgattta gggaataaga aaaaaataga gattgtcaat gcattcttac
    39361 ctgtcaaaca cttaaatgta gaccgtgttt cagaaacgat ttcttcttta gaaagagaaa
    39421 gagcggaggt cttggtttct tctatggcat ttggagtaga taatggggaa ccagaacgag
    39481 ttgtttatga agcagcatcg gtaaaatcga tccctacaac gatggcttct gacatcacaa
    39541 aattatatgg attgacaaga agaactcgga cagcagcaat caatgcatct attttaccaa
    39601 aaatgctaga tacggcaacc tctacagaag attccgtacg agaagcaggg gtcaatgttt
    39661 ctttgatgat tatgcgcgga gacggcggag taatggaaat caatgaaatg aaaaaacgac
    39721 ctgttttaac catgctttcc ggtccagcag catcagtcat gggctctttg atgtacttga
    39781 gagcgtctaa tggtgtttat tttgaagtag gcggaacaac aaccaatatc ggggtcatta
    39841 aaaatggacg tccagcaatc gattattcaa ttgtaggtgg ccacccaaca tatatctctt
    39901 cattagatgt tcgggttcta ggtgtggcag gcggttcgat ggtacgggct aatcagtcag
    39961 gaatcatcga tgtcggtcca cgttctgcac atattgccgg tctagattat gcagtattta
    40021 cggagacaga aaaaatcaaa ggtcctaagg ttgaattttt ctctcctaaa gaaggagacc
    40081 ctgctgatta cgtcaaggta gtgatggaag acggagaaga agtgactatt acaaatacat
    40141 gtgcagccaa tgtccttgga ttagtgcaag aagaacattt ttcttatgga aatgtcccat
    40201 ctgcacgaaa agccatccaa gcattagctg attattgtca tacgacggtt gaagacatcg
    40261 cagaacagat catggaaaaa tcctatgcta agattgaacc ggtgatttta gaattggcag
    40321 acaagtatca cttggagaaa gatcagattt ctcttgtagg tgtagggggc ggcgctgcct
    40381 cattgattac ttactttagt aataaaatgg gtgtgaaata ttcgattcct gaaaacgccg
    40441 aagtgatttc ttctattggt gtagcacttg caatggtccg agatgttgta gaacgtatca
    40501 tcccttcgcc aagtaaagaa gatatacgat cgttgaaaaa tgaagcgatg aataaagcga
    40561 ttgaatcagg cgctacacca gaatcgatcg aagtccacgt agaaatcgat ccacagactt
    40621 caaaagtaac ggctatcgct actggttcta cggaagtgaa agcaaccgat ctaaccaagg
    40681 agatcacaac agaagaagca ctagagcttg ctgcagagga tatgcgatta aataaaaatg
    40741 aagtttgctt attggaaaat acacctttct tttatgtatg cggcgaacag aatcgttcaa
    40801 aaaatgctgg aagtcttcgg atcatcgatc aaaaaggatt tatcaaggtc caacgtggac
    40861 atgcttcatg tatgaaaaca actgcagcca attatatgac agctgtcgaa caactttggg
    40921 aagatatggc agtttatcaa actgaattga ttgcccgacc agaattttat ctctgcttag
    40981 gagcaagagt ctctgatttt acagcgacgg atctggaaca actacagctt ttgatggatc
    41041 tggaagtatc tactatggaa ccggaggaag aagtgatcgt cgttgccgga aatatcaaac
    41101 aaacataagt tagaagagtt actaaacgaa ttacaacaga tccccgaaga aatttgggga
    41161 ttctaccagt ttcggcgaga tttattttgg aaaaagattc ctttgtccaa gcagaaaatt
    41221 cttatacaac aatcaatcga ttgtggtatc gaaacggcat gtagcatcaa aaaaaagtac
    41281 ccttttgccg atgtaggaga aatttgtgaa caaatggctg ttccaatcgt ttcctgtgaa
    41341 tcagagcaaa taaacgaacg aatcactttt gcaacttatg cagaagatga agggattcga
    41401 ttgatgacgg agccgttaga aaaattaaaa tgttcaggac tagcttcgat ctctaaagaa
    41461 acagcgcaag ctttgattat tggacatgaa ctatttcacc acatcgaagc ttctgttaag
    41521 ggaatttata cgcaaagcga aaaaattgtg ctgtggcggt tgccttttta tacacatcaa
    41581 tcaaacattc gtgcattgag cgagatagca gcgatgagtt tttcaaaaga gatgaatcaa
    41641 agcgattttt cgccttatgt attagaggca gtcttacttt ggccatataa tgaaattcgt
    41701 agccaaggga tcttagaaga aataaaaaaa atagaaaaaa gatgtacgga gtatgattta
    41761 gattacaaat aaagagactg tgacaaaagt cgtgtcacag tccttttttt ccgaataaac
    41821 gatgttctcc tgtcaactcc tcggtcacaa gtcaaaatat tcaacaaaca tgagaagctg
    41881 ttttttgaaa ttgttccttg tgactcggag atgaacggca ggctcacgac ctcttggaaa
    41941 acggtgttca aaagcttgca tctgcggtaa taagtccagc caagttaaaa acataagaag
    42001 ctatttttac ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcaa
    42061 taaacggtgt gcaatcagct gaccctcggt attagctcaa aaaaaacggc aaaatatgag
    42121 gaactatttt gccgtttctt tctctaatac tcaggtcaaa acgctgattc caaaacctct
    42181 tatttgatgg gtctgtcctg aatatcttca aaataatcgg ggtaattatg aatcacgtat
    42241 tcttgctcct cgataatcat atgtaaatgc atcgtgcaaa gaaatccgac ttcatcaaag
    42301 ttatgatcaa tcattgattg aagcattgtc tgatgttcat ctaatactcg accccagtct
    42361 aattctgaaa tcgtcaaacg taaccatctg aaacggttca agtgcgtact ataactttct
    42421 agccaatccc aaatttctcg ttttccagca atttcataac aagttcggtg aaaatcagta
    42481 tccaaatcaa aaaaagcttt tttattttga gcgattactg cctgatcggt ttgttctagt
    42541 attttttcta gtttagcttg atcttgcttg gtcattttcg cactgcattc ttgcaggatc
    42601 ggtttttcca acttttcccg cgtataacgg gctaatgaag cagatcggat atcgattttt
    42661 gaaacataag tgccggactg tggaatggtt tttacaagct cttgattgcg cagttggatc
    42721 agtgcttctc gaatcggtgt acgcccgata gctagttgtt cttctaacac tttatcggag
    42781 attttacttc caggtgctaa ctcggaataa atgatttgtt tacgtataga ttcataagct
    42841 tgctgttgca aattattgat aaccatttaa ccttcctttc tatgcagata taatctatta
    42901 taacataaat cgcttacaaa tcgtgtgggc ataaaaacac agtctgcaaa aaaagccctg
    42961 ataggcatgc ctattagagc ctcgaaaaga aaaatattga atcaatctgg tcgtacctct
    43021 tgtatcggtt tgacacgacc gccgattctt ttagcaaaag cttcggattc ttttttgtct
    43081 ttgaaaatga gcagattgtc taagtttttt tcccgagtgc gacagccagt tttatcgatg
    43141 taaccgtgca ttactttaac gacatacatg gggatcacct gcttttttct tttcattata
    43201 cgaactactc gacacaatac aagaaaaatg cttagacggt ctttattaag aatttacatc
    43261 atccaaagaa gcgtacgctt ggaataactg atctaactcc ggattgactt tgctcagctt
    43321 cactaatcgt tgcgaatcag aaagtaaaaa acagtgttta ctactgccag tagtttttaa
    43381 ttttttgtct tcagttccca caatttcgta gctaaaatcc aaccgtgtcc ccgtatactt
    43441 ttggatagtt ggatggatgc tcactttttc tccgtaatgg atcatttctt tatagctgca
    43501 atttacttcc agaacgggga tgatgatccc agattcttcg atttttttat agggaaaccc
    43561 tagatgtacc aataaggcta cccgagcctc ttcaaaccag cggatataat tgctgtgatg
    43621 aataatcccc atttgatctg tttcataata aaaaggttct cgcacataac ctgtaaattt
    43681 ctccatgact catgcctcgt tttccattga ttttttacaa aaataattca tccatttcgg
    43741 aagtgcttcg ctgatttgtg tcggcaaaac aatatattgt tcttttgcca gctggtctcc
    43801 aatgaaacta tgaagataaa tagctgctgc aatcgtttgg tcattttgag gaaactgggc
    43861 taaaaatcct gtgatcatcc cagctaaagt gtcacccatg ccgcccgttg ccatagcagc
    43921 agaacctaaa ggattggtgt agaagtgctg gtgaccataa atttttgtct catgactttt
    43981 caaaacgagc acagcaccga tttcctgttg cttctttttg ttttctttat cccgttgttc
    44041 ttgtatcggt agagaactca accgttgcca ctccatttga tgaggtgtaa gtacagtttg
    44101 ttcgggatag ggtagcaaaa gatgattagc agcaagtaaa cttaaagcag agccatcaat
    44161 caccagccat tgttcttttg attggttttc tagtacttgt ttgaaacgtt ccaaactctt
    44221 agaagaagta cccaatccgg gaccaatcaa gagtacatct gcttgcttta ttacggaaag
    44281 aaactggtca gtatccgtcc aaactacaat cattgcttct ggcagatgac tgtgaagagc
    44341 actccgattt ttttcatcag ttactacagt cgtgagaccc gctccgctgt gtacggcagc
    44401 taaagcactc ataatgattg ctccgccata ttgttcattg ccgccaatca aaacgatacg
    44461 tccaaatgtt cctttatgtg aaatttttgg acgaggtgtg atgactcttt tcactagttg
    44521 ttcgtttatc agcatctaaa aacctccttt tactcattat agcgtatatt ttttttagag
    44581 aaaaacagat aagaaaagga attgtcagat tttttgaact atttttttct gtaatttgtg
    44641 gtatcatgaa cacgaagact taaataagtg gaggattaac tttatgacaa ttgattggca
    44701 aaaagaagta gaagcacgta aagaagactt gcttgaagac ttgaaaaatc ttttacgtgt
    44761 caacagcgag cgcgatgact caaaagtaac accagatgca ccatttggac ctggacctcg
    44821 cgacgcattg aaacatatgt tagcttatgg cgaacgtgat ggatttaaag taaaaaatgt
    44881 cgataactat gcagggcata ttgatcttgg cgaaggagac gaaacgctag ggatatttgg
    44941 acacatggat gtcgttcctg caggagatgg ctgggacaca gatccttatg aaccagtaat
    45001 caaggatgga aagatctttg cacgcggatc aagcgatgac aaaggtccaa gtatggcagc
    45061 ttactatgcg atgaagatca ttaaagaact tgacttgaaa ctatcaaaaa aagttcgctt
    45121 cgtagtcggc agcgatgaag aaagcggctg gggcgatatg gcttattatt tcgaacatga
    45181 agaagaacca gattttggtt tctcaccaga tgctgaattc ccaatcatca atggtgaaaa
    45241 aggaaatgtt tctttagctt tacgcttcaa aggcgacaat gcaggagatt atgtcttgaa
    45301 atcttttgtt tccggacttc gcgaaaatat ggtgccagga acagcaactg cagcacttca
    45361 agtaccaagt gcagatgcag ctatcgcgat ggaagaagca ttttaccaat tcatcgaagc
    45421 aaatccagta agcggaacga ttgaagcgga caatacgtat gtaaaaatcg aattagttgg
    45481 taaaggcgca catggtgcaa gcccacaatc tggtatcaat gcaggttctt tcttggcatt
    45541 gttcttagat aactatgaat tcctaggatc tgctaaacaa tttatccatg tagcagctgc
    45601 ttatgtccat gaagattttt atggagaaaa actaggagtc gcttatgaag atgaaaaaat
    45661 gggcaaattg acaatgaatg ctggactatt tgcttttgaa gaaaatggta cagaggaagc
    45721 gaacttcatc aatatgaatt tccgttttcc aaaaggtgta acagtagacg gattgcaaag
    45781 tgacatcgaa caaacagtgg ggcaagaagg cgcaacagtt actcgaggtg ctcgcgtaat
    45841 ggaaccacat tacgtaccaa tggatgatcc attggtggct acattattgc aagtctatga
    45901 agaccacact ggagaaaaag gctacgaaca aatcatcggt ggcggaactt acggacgttt
    45961 gttgaaacgt ggcgttgctt atggagcgat gttcccagga tatactgata caatgcatca
    46021 agcaaacgaa tttatgagtc ttgatgactt gttcagagca acagcaatct atgcagatgc
    46081 catctatcgt ttggcgaaat aattaattaa aaagaggatc aagaattgca aattattttt
    46141 gcagtcttga tccttttttt actttctact gtggtattca tgataaacat gatatagatt
    46201 caattcatat tctttcttag cgttcgtgac gactgtatcc aaaatcaacc ctgttactaa
    46261 tgaaagaagt gctaaagtca tcaatccagt acttaaaatc gcagaaggga tgcgtgtaat
    46321 aaagcttgtc agcatatatt cgcgaatcac aggaatcccc gtgaccaatc caaataaaaa
    46381 gaagaagatg gtccaaatcc caaagaaaag caaaggctta taatctttaa acattttaat
    46441 aatcatcatg atcactttga atccgtcaga gaatgtattc aattttgatt cgctgccttc
    46501 aggacgatca cgataatcga ttgggatttc aacgaacttg aattttttat ctaaagcatg
    46561 gatcgtcaat tccgtttcga tttgaaaacc agaactcata ataggaaaac ttttgacaaa
    46621 gatacgattg aatcctcgat agccggtcat gacatcaaga atttttgttt tatacattct
    46681 agtaattgta tttctaacaa ggttatttcc aaaatcgtgg aaaggtctct tattctcatc
    46741 aaagtaggtt ccatttgata ggcgatcacc aatgaccata tctgcttcac cagaacgtaa
    46801 tttctcgagc aaaccatgta cagcttctgc aggataagta tcatcgccat ctaccattaa
    46861 ataatagtcg gcatcgatat caaagaacat ttggcggatc acatttcctt ttccttgacg
    46921 aggttctttt tttacgattg ctccaccttg aacggccaat tcgtatgttc gatcggtcga
    46981 attattatca tagacataaa tatctgcttc tggtaattct cgtttaaagt ctgcaatgac
    47041 tgttgaaatt gttgcttcct cattgtaaca aggaatcaga acagcaattt tttcttgttg
    47101 ttccattttt cactctcctt aactatagaa aacaaaagct aaattaaaaa tacctcataa
    47161 tcaaaggaaa tgctagtttt tcccgctaaa tatcatttat ttaaaaagaa aatactggaa
    47221 tatatgtttg gaaagctcta tctaaaaaac cacccactac aaaagaagga catgatacct
    47281 ctcttttgaa tagtgggtgc gtattcgttc tctcactttt ttgacgtatt aatctgttgc
    47341 accttttttt cttgaaattt ctaatacggt tcctgtatga gcatctgcta aaaattcata
    47401 ataaacagtt tcgccatctt ctattcgaga aagacctccc cgataagcac tcgaccgaac
    47461 ggcgaatttc ctaataggtt ccttttcaaa agagatccaa gaaccttcga ttgcaccttc
    47521 ttttagaaaa gatgacttga tattttcaag tacttggtct gctgacaata cttttttgcg
    47581 atgataccaa atcgttgaag ctaccccgct caataatcct agaccgattc caactgctaa
    47641 cccgccttta aaatagctta gttcgctttc ttcatacatg tggacatacc tccttcttct
    47701 cacttgttac tccttgtgct tattttacca cactctcata ttttctttga aataaataac
    47761 cgctaccaga aaaaataagc tataataaat acgatcggtc tgcagaaaaa atcatatttt
    47821 atccgcagat caatataagt ctgagaggag tttagtctga aatttagacg aatagcatat
    47881 ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa ttacaaggaa caagcggttt
    47941 tgaacaagat gttcgcgctt atatggaaag tcatatggaa ccgctggtag atgagttaca
    48001 gttagatggt ttgggcggca tttttggtct gcgccatcat gaagaagcgg atgcaccccg
    48061 tgtaatggtc gcagcacata tggatgaagt cggattcatg ctgacacaaa tccaagaaaa
    48121 cgggcttttt aaagtcgtac ctctaggcgg ctggaatcct tacgttgttt ctgctcagcg
    48181 ttttacactt aaaacctcta ctggtaagaa ttatccatgt atttcttctt ccgttccccc
    48241 acatttattg cgaggaacaa gcggacaaaa atcagtagaa gtaaccgata ttttgttcga
    48301 tgctgggttt gaatcaagag aagaggcaat gagttacggt gtccttccag gagacacaat
    48361 cgttccttac gctgaaacga tcaaaacagc caacggaaag aatattatca gtaaatcatg
    48421 ggacaaccgc tatggctgta caatggtatt agaagcttta gaagcgttgc aaaatgaaac
    48481 attaggtcat acattgattg caggcgcgaa tgtccaggaa gaagtaggct tgcgtggttc
    48541 taaagcttct gtcaataaat tcaagccaga tctgttcttt gcagtagact gctctgcagc
    48601 agatgataca gtgacgaaaa atggcacatt cggacattta ggagaaggta cattgatgcg
    48661 tatccaagat ccaggtttga tcatgcttcc tcgtttgcgc gagtacttgc tggatatcgc
    48721 tgaaacaaac aatatccctt atcaatactt tgtttcaaaa ggcggtacag atgcaggtgc
    48781 tgctcataca caaaacgaag gaatcccaag tacagtgatt ggcgtagtgg gacgttatat
    48841 ccatacgcat caaacgatgt tcagtattcg cgattttgaa gctgcacgag aaatgttgat
    48901 tcaaacactg aaaggtttgg ataaatcaac agtgaatacg atcgtatacg gaaaataatg
    48961 cgatagtgaa actataaaat ctggcaaaag attgcttttt gccagatttt ttttataaaa
    49021 agaggatcaa tgtttgttag ttcgaaagaa aggtgtataa tttaattgaa aaatacataa
    49081 acagaaagta gaggacaaag aatgattatc ccaaatagtt tagaagaatt agcaggctac
    49141 gtagaaactg gaaagagtgt atttttcttt actgccggct ggtgcggaga ctgccgtttt
    49201 atcaaaccgc aaatgcctga aattgaaaat gacttttctg attggcaatt tattgaagta
    49261 gaccgtgaca agtacataga tgtagccgct gaatggaaca tttttggtat cccaagtttt
    49321 gtcgtgatcc aagacggaaa agaattaggt cgtctagtga ataaagatcg taaaacaaaa
    49381 caggaaatcg aatcgttcct agaaagtgta acaaaaggct gacatctgtt aggaggggtc
    49441 aagatgaatc aagaatataa gaatatttta gtaggtatcg acggtagtga acaagcactt
    49501 gctgctttca aaaaagcggt agaagtggct cgccgtaata atgggaaagt atatgtagca
    49561 aacgtaatcg atcagcaatt ttataacttt atgggctatg cacctattga tcaaaatcta
    49621 gtcgatcagc aaactgaaac agccaaggaa atgatcgaag aatgtaaaga atatgggaaa
    49681 tctgttgatt acacaaatat agaaggaatt attgcatatg gttctgttcg tgaatccatg
    49741 gcagtcaaat tgccaaaaaa atatgagatc gatctaatca tggttgggca gtcaggtcta
    49801 aatgcagtcg aacgctttat gacaggaagt gtagccagct acgtgatacg tcattcacct
    49861 tgtgatgtct tgatcgtttc tgacgaagaa gaacagccaa gtaattaagc aagtggaact
    49921 tgtacgacct ttttaatcaa ttaactaaaa cagtaaaggg actgtgacaa aagtcttgtc
    49981 acagtccctt gttacgaata aatggtgttc cagaagaggc ttcttcggaa ataaagcatg
    50041 aaaaaaattt catgcagtga atgcaatttt ttgttaagat aaatctcagc aaaggataat
    50101 ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac cttgatggtt
    50161 attatagccg acgataaagg aacagaaaac actgttgaac gcaaatggaa tgttgctcgg
    50221 atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc cgatcatcta
    50281 acaatcgaag ggaacggcca agtaagctta acagatgagg aattagcgaa attgaatcga
    50341 ttgatcaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc gaagatcgtt
    50401 gtcgggtacg taaaaacatg cgtcccacat cctgattctg accatttatc gattactgag
    50461 acagaagtag acaatggtac agtattgcaa atcgtttgcg gtgcacccaa tatcgaagct
    50521 ggacaaaaag ttgtcgttgc aaaaccagga gctatgatgc cagatggaat gatgatctgg
    50581 ccaggaactt tgcgaggggt agatagctat ggaatgatct gttccgctcg tgaattgcat
    50641 ttgccaaatg ctccagcaaa aaaaggaatc ttagtattgc ctgaggatgc cgtagtcgga
    50701 gaagcatttc caaaataaag aggttgtgac aagtctttgt cacaacctct tttttattaa
    50761 ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc aacagtagtt
    50821 gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac ttttttctta
    50881 tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat cttagtaatg
    50941 acgtcgtatt gtttcaatcc agctttttca gccggtgtag ctgtttggac atttgtcacg
    51001 atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg ctgagtagaa
    51061 acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt tccgtctttt
    51121 tccagttgat tgatgatatt cactacatcg ttacttggaa tagcaaatcc cattccttct
    51181 acgcttacat tagaagtaga ttcagaagta cttgcgatct tacttgagtt gataccgata
    51241 acttgtcctt cgatattaac taaaggacca ccagaatttc cagggttgat tgctgcatct
    51301 gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt cacttgtcgg
    51361 ttcaaggaag aaatgattcc tgaagtgaca gaatttgcat attcagatcc tagtggggaa
    51421 ccaatcgcaa tcgcaggctc accaaccttc agagcacttg agtcaccaaa agaggcaact
    51481 gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataagc atctgttcca
    51541 actaattcag cttttacctt cgtaccgtct ttcatcaaga cttcaagacc ttgctggcca
    51601 tctactacgt gattattcgt gactacataa gcagtattcc cgtctttttt gtagatcacg
    51661 ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact ttcttcttgc
    51721 tgcccgaata gtccgccaaa accgcttgat tggttttgac tttgtaagtt gataacagaa
    51781 acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac attgactttg
    51841 acattttcca caactgtttc accggcagaa ttctgattgc cgcttgtagc agtagaacta
    51901 ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc tccaacgatc
    51961 cctccaacaa gccccagacc taacttacgc cagaggctat tatttttact ttttttcatc
    52021 tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca tttatctact
    52081 gttagtatag tcttatttgt tgactttagt ctactttgag actgtgaact ttttatgaaa
    52141 aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt tccacagata
    52201 ctgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat ccacagcctg
    52261 tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt gatgaaataa
    52321 ggtaaaataa cactgttttc tgtggataaa cctgtggatg ttgtgcataa ctgttgggga
    52381 aatcttttta acaaaaatag ccaaaataaa catatagacg aaaaaattga ttataataaa
    52441 caaagtgagc atgaagacca aaggagagaa aacatgaata tcaaaattat ctcagtagga
    52501 aaattaaaag aaaaatacct gatccaaggt atcaatgagt atgtcaaacg gctgaatgcc
    52561 tatgcaaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa tttaagcgaa
    52621 gcgcaaatga gacaagtaaa agaaaaagaa ggggaacgga tcttagcgaa aatcaaagaa
    52681 caagaatacg tctatgcctt agcaattgaa gggaaaaatc ctacaagtga atcttttgct
    52741 aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt tatcggcggc
    52801 tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc ttttggaaaa
    52861 atgacgtatc cacatcaatt gatgcgtctg atccttgtcg agcaaatcta ccgtgccttc
    52921 cggatcaatg cgggggcgcc ttatcataag tgatgcggtt tttatcattg atcctaaaaa
    52981 tcttagcaag acaaaaagta cgtaagaaag taaagactaa atacttcgga ggaacaaatg
    53041 aataacaatg ataggttatt gcgattgcgt tatgcgattg atttgaaaga tacggatttg
    53101 attaaagctt ttgagctagg cggtgtacca ctgactaaag aagaagctca ggcggtactg
    53161 accaaagtac aggataagaa caaagataat gcagaaaata atgtgtatga aaaaaccatc
    53221 aacaaccaag tttttgacgc attcctaaat ggcctgattc tcttggcgcg tggtccgcaa
    53281 aaagataagc cgatagtaga cacgccgcaa aaaagcaagg aaatcaaata tatcaacaat
    53341 gtgttgctaa aaaaattaaa aatcgccttg tctatgacca ctgatgatat tttagacgtt
    53401 tttgctgagg cagaaatcta tccatcaaaa ggcgaaatcg gggcattttt gagaaaagaa
    53461 ggccaacgaa attttaagcc ttgcggagat aaatatatgc gtaattttct caaggggtta
    53521 ggaatttata atcggagaaa agtataaaga aatttcatga gaaatcaaaa caatggttgt
    53581 tttgatgaga gctagttttt gtatgagatt aaaatattat cttaatcgag gagctggatt
    53641 ggttttatga atgatgaata taagaatgat gaagataaga tgctttttga agagatagag
    53701 aatcgatgta ggctcaattt tgagcttcgg ggaaagatgt ctttaataca acaaaaaaag
    53761 tatttagcga ataaatcaga gtttactctg ggacatgttg aaaaattaat ctcggattgg
    53821 attagtagta gaagcgaatt tacaaaaata aaacagccga ttaaatttga catgaaaaaa
    53881 cttttgttaa ataagagtga gataggtaat agagatcaat atattagagc taaagggcaa
    53941 gaaataattg attcattagg tgaaatgagg tcatacaatt atttgtatgt aacacataga
    54001 gctgatggaa tggtcattac tgtggggaaa tcttcttcta atgatatttt tttagatgga
    54061 gatctttttt atcaactcaa tacaaatcac ttgtctggca ctgaaaatat aattttaagg
    54121 acggaatatg gcaatgaaat atttgcaaaa tatgatgaga ttttaaaaaa ttatttggac
    54181 tgggcttgga taattccggt tgagtcagga gatgctaaga aactggagag attattgggc
    54241 gatgaactaa ttaataaaaa agtaccaatt ttaaattatt attcccatag acaataaaag
    54301 gctatttttc atgataatac tagttataac tgtaaaaatg gaagaaaatc tgggaaagaa
    54361 gtttttaaat aagtgtattg ttcgatttta ataaataata agcaatacca tccgtctgtg
    54421 ttgcgcaaca tcgtactaca atgaatattt tttcacaaaa agaaaatcat ccatcgaatg
    54481 aatggaaggt tttagtatac acgaggttaa tgccccctct tttataatca gaggagtgag
    54541 gttttttcta tgaatgtttc tattttctta ttgctagtct agtttctctt acgatgaaat
    54601 tgggattcta ttccagtttt tattgatttg ctgataaaat aacttgaatg gtggatataa
    54661 acatgataaa taagagaaga ataacaaaac aaggttgtga aatgattctc aaatgaatat
    54721 aatagaaaga aaaaggagga agaatcaatg aaaatagcaa ttgcaggcgc tggcgcaatg
    54781 ggaagtcgct ttgggttaat gcttcaccaa agtgggaacg aagtattatt aatcgatggc
    54841 tgggcagaac atgtccaaca aatcaaagag catggattac aagcaaattt taatggtaaa
    54901 gaggtagaag caaaactacc aatcgttctt caatccgaag tagaaaaaga agatcaagtt
    54961 gatctgatta ttctatttac caaagcgatg cagctggaaa aaatgctgca ggatatccaa
    55021 tcattaatca aaaaagatac agaggtctta tgtctattaa atggtatcgg gcatgaagat
    55081 attattgaga aatttgtacc aatggaaaat atctatattg gaaataccat gtggacggca
    55141 ggtcttgaag gtcctggtca ggtcaaatta tttggaagtg gttcagtaga attacaaaat
    55201 ttaggtgatg gaaaagaagc agctgcaaaa aaattagcag acaaattgtc tgaatcagga
    55261 ttaaatgctc atttttctga caatattcac tattctattt atcgcaaagc atgtgtgaac
    55321 ggaacaatga acggactatg cacaatctta gacgttaata tggctgagct aggaaaaaca
    55381 tcgactgctc ataaaatggt ggcgacgatt gtcaatgagt ttgccaaagt agcagcagta
    55441 gagaagattg aactagatgt cccagaagtc attgcacatt gtgaatcttg ttttgacccg
    55501 gaaacaattg ggttacatta tccttcaatg tatcaagact tgattaagaa ccatcgatta
    55561 acagaaatcg attatatcaa tggcgcaatt tctagaaaag ggaagaaata tggtgttgcg
    55621 acgccttatt gtgatttctt gactgagctt gtccatgcaa aagaagatag tttgaacgta
    55681 aaataaagga gagattgaat ggaaacttca caaaaaatat caccgaaaat ttttctgaat
    55741 aaagtcctcg ctggtacagc aacaggaatc attattggat tgattcctaa tgcagtatta
    55801 ggtgccattt taaaatattt tagtgcgtat ccctttgcgg taacgatcgc acagttagca
    55861 gttatttttc aactagcaac acctttgatc attggtggtt tgattgcctt acaatttgga
    55921 tttgatccga tgagaatgat ggttgtagcc ggagcttctt ttgttggttc tggtgtagtc
    55981 aaattcacac ctgatttggg tgaaacgggt gtttacgttg gtgctggtac aggcgatatt
    56041 attaatacga tgctgacagc atcgattgct gtcgggctga ttttactgat tggaaatagt
    56101 tttgggtcag tgtcgattgt gttaacaccg attgttgtag gtgtaggtgc aggtttactt
    56161 ggtttttatt tattcccttt tgtaacagca attacttctg caattggatc attgattaat
    56221 aacttcacta ccttacaacc gctgttaatg tctatcttga ttggttgttc ctttgccttt
    56281 ttgattatct ctcctatttc aacagtagca atcggaatgg caatccaact agatggtgtt
    56341 tctgctggtg cggctgcgat gggcgtagct gcaaccactt ttgttttagt agtgaactct
    56401 tggaaggtta ataagtcagg tgtgacaatt gcgattgctt taggcgctat gaagatgatg
    56461 atgcctaatc tatttagaaa acctgtgatt cttattcctt gtttagttag tgcagtgatt
    56521 acagctattc ctgtcgctct attttctatt tcaggtacgc ctgcttctgc tggtttcggc
    56581 gtggtcggac ttgttggtcc gttggcttct ttagatgcag ggctaaatgg ctttcttgtt
    56641 gttctttgtt ggttagtcat ccctgttttt gcggcactaa ttactcaatt tttatgcgag
    56701 aaagtattta aactgtatga tcgagaagaa gtcttcaaat ttttaggcta gtagatttta
    56761 ctaagaaatt ttcaatcaat ggatgcgtaa gtgagatgtc aacaacatct gacttacgtt
    56821 tttttgcctt tttgagtagg agattgatgc tgacaactga actcttcagt gccattcgat
    56881 tttttaaaat gtagaagaaa ctcaccttta tgttattgat tgttcaaaag gcaactagaa
    56941 taaaaatatg tgttaaaatg agaaaaaatt tgattgttgg atgtatcgaa taatcactaa
    57001 aaagtaggag aagaaaatga tttttttgca aaaatatggt ttttattttt tattattagg
    57061 tgtgattagt gatttatcga caccgtatat ccttggcctt ttttatccca aactgaatca
    57121 aatgactacc gtaattagtg tgtttggtga cgtagatagt ccagtcagac gtgctttctt
    57181 ggtctggtca gttgtatctg gacttttttt tgttctttcg ttgcctgcgt tgtatcacct
    57241 atttattgga acatcaaaaa cacttgcaat acttgctgtt gcaacagttg gtttatatgg
    57301 gataggtgat tgtattttta ctgggttatt cagtatcaat acgaatgaaa gctcttggaa
    57361 tctatcgact tggatccata atataggttc tgggctaggc tatgcaggct ttctactttt
    57421 cccattgtta cttgttctgc tttatcgtca aagtggtcaa ggtacgctta gccattttta
    57481 tcttgtttta acagttatta gtttactaat tgcaggtctt tacggattag cacggattcc
    57541 gtcgattagt cagtttgcct tctttaagca attgggcttc tttcaacgct taagtttttt
    57601 ctttaactat ttgccgatga tttgtttaag tttttttcaa ctacgaaagt agaaaaagca
    57661 gtgtgaagct gattaagtaa aagaatttct ctatgggtaa ccattcgtgt ggatgtgttc
    57721 gactaagaat agagatgagg agagcgaaaa gcaagttagt cttaacttgt tatgcgttgt
    57781 gctaaatttc aggtagtcat tttttgaatg aataagaaga aaacttgaaa gagggtgact
    57841 tcatgttaga tgtggagcag atggaaatag acctttataa tattatttta aatcgaaata
    57901 caagagaatg ggaaagaaca caatttgaag aggcaaaaga agacctagca aaaatgtcga
    57961 taaagcaggt gatagaaaaa ctagaggtca acttgcgtcc gctagcacta cgagataatt
    58021 tgacaccaga tgcgatggat ttttatttaa gaatcacagg ggagtcagcg agtgcaaagg
    58081 cttatgatta tgccaaacat aagataactt cacccttata ccaagaacgg gcgatttttg
    58141 ccggtggctg tttctggtgt atggttgagc cttttgaaac aaaaccaggt gtaatctcag
    58201 tgttatctgg ttatacaggt ggagagacca aaaaaccaaa ttacgatcaa gtcagtggtg
    58261 gatatactgg gcatgttgaa gctgttgaga tcatatttga tacacgtcaa ataacctatg
    58321 aagagctgtt ggagatttat tggcaaattt cagaccctac agatgccttt ggtcaatttc
    58381 aagatcgggg gaaacaatat cgttcaattc tatttgttgt taatgaaaag caaaggcaat
    58441 tggctgaaaa atcaagagaa caactgattc aatctaaccg atttagccgt cctatcgtta
    58501 cagagattcg ccaagcaaag actttttggc cagctgaaaa tcatcatcaa caattttatc
    58561 agaagcaacc taaaagatat aaaaagatca agcattcgca tcagcttcat ttattttttc
    58621 agcgacatag atagaggaag gttgtggcgc aaagcgttca gctccgacaa aataatgagc
    58681 aactgacaaa aatagctttt catatttttg gacagttatg aattatttcg aggagttgct
    58741 ttgagaacac cgaggaaaag gttgtggcgc aaagcattca gcttcaagtc gcaaggaaac
    58801 aggaggtatg acaagtcttt tgtcacaact cctgtttttt tactagcctt tatttccatg
    58861 tgaaagagaa ttcgaattgt agaatcttga tttttctatg gaattttgta gagaatcatc
    58921 ttgataatta atagacacct aatcgaattc cacattaatg atgaactaaa ttccgtataa
    58981 aagcgcaaac aataactata aaacccg
//
LOCUS       DACDVT010000011        57439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDVT010000011 DACDVT010000000
VERSION     DACDVT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.011"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..100)
                     /locus_tag="IWL52_000657"
     CDS             complement(<1..100)
                     /locus_tag="IWL52_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9777243.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGII"
     gene            complement(561..875)
                     /locus_tag="IWL52_000658"
     CDS             complement(561..875)
                     /locus_tag="IWL52_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9777244.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(891..2486)
                     /locus_tag="IWL52_000659"
     CDS             complement(891..2486)
                     /locus_tag="IWL52_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP9777245.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(2509..3399)
                     /gene="iolE"
                     /locus_tag="IWL52_000660"
     CDS             complement(2509..3399)
                     /gene="iolE"
                     /locus_tag="IWL52_000660"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP9777246.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(3416..4441)
                     /gene="iolG"
                     /locus_tag="IWL52_000661"
     CDS             complement(3416..4441)
                     /gene="iolG"
                     /locus_tag="IWL52_000661"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP9777247.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(4465..5472)
                     /locus_tag="IWL52_000662"
     CDS             complement(4465..5472)
                     /locus_tag="IWL52_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9777248.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(5585..6745)
                     /locus_tag="IWL52_000663"
     CDS             complement(5585..6745)
                     /locus_tag="IWL52_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP9777249.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(6821..8275)
                     /locus_tag="IWL52_000664"
     CDS             complement(6821..8275)
                     /locus_tag="IWL52_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP9777250.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            complement(8339..9163)
                     /gene="iolB"
                     /locus_tag="IWL52_000665"
     CDS             complement(8339..9163)
                     /gene="iolB"
                     /locus_tag="IWL52_000665"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP9777251.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(9202..11118)
                     /gene="iolD"
                     /locus_tag="IWL52_000666"
     CDS             complement(9202..11118)
                     /gene="iolD"
                     /locus_tag="IWL52_000666"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP9777252.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(11129..12163)
                     /gene="iolC"
                     /locus_tag="IWL52_000667"
     CDS             complement(11129..12163)
                     /gene="iolC"
                     /locus_tag="IWL52_000667"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP9777253.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(12320..13336)
                     /locus_tag="IWL52_000668"
     CDS             complement(12320..13336)
                     /locus_tag="IWL52_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9777254.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(13607..14926)
                     /locus_tag="IWL52_000669"
     CDS             complement(13607..14926)
                     /locus_tag="IWL52_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP9777255.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(15667..16647)
                     /locus_tag="IWL52_000670"
     CDS             complement(15667..16647)
                     /locus_tag="IWL52_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9777256.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(16660..17538)
                     /locus_tag="IWL52_000671"
     CDS             complement(16660..17538)
                     /locus_tag="IWL52_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9777257.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(17554..18354)
                     /locus_tag="IWL52_000672"
     CDS             complement(17554..18354)
                     /locus_tag="IWL52_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP9777258.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            19746..20072
                     /locus_tag="IWL52_000673"
     CDS             19746..20072
                     /locus_tag="IWL52_000673"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9777259.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            20080..20988
                     /locus_tag="IWL52_000674"
     CDS             20080..20988
                     /locus_tag="IWL52_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9777260.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            21617..21868
                     /locus_tag="IWL52_000675"
     CDS             21617..21868
                     /locus_tag="IWL52_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP9777261.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            22410..22568
                     /locus_tag="IWL52_000676"
     CDS             22410..22568
                     /locus_tag="IWL52_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9777262.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            22640..23866
                     /locus_tag="IWL52_000677"
     CDS             22640..23866
                     /locus_tag="IWL52_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9777263.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(23955..24347)
                     /gene="rpsI"
                     /locus_tag="IWL52_000678"
     CDS             complement(23955..24347)
                     /gene="rpsI"
                     /locus_tag="IWL52_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9777264.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24363..24806)
                     /gene="rplM"
                     /locus_tag="IWL52_000679"
     CDS             complement(24363..24806)
                     /gene="rplM"
                     /locus_tag="IWL52_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9777265.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25325..25792)
                     /locus_tag="IWL52_000680"
     CDS             complement(25325..25792)
                     /locus_tag="IWL52_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9777266.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(25912..27450)
                     /locus_tag="IWL52_000681"
     CDS             complement(25912..27450)
                     /locus_tag="IWL52_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9777267.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLSGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27530..28216
                     /locus_tag="IWL52_000682"
     CDS             27530..28216
                     /locus_tag="IWL52_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9777268.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28417..28944)
                     /gene="msrA"
                     /locus_tag="IWL52_000683"
     CDS             complement(28417..28944)
                     /gene="msrA"
                     /locus_tag="IWL52_000683"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9777269.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29028..29705)
                     /gene="pgmB"
                     /locus_tag="IWL52_000684"
     CDS             complement(29028..29705)
                     /gene="pgmB"
                     /locus_tag="IWL52_000684"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9777270.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(29702..30832)
                     /locus_tag="IWL52_000685"
     CDS             complement(29702..30832)
                     /locus_tag="IWL52_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9777271.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(30832..33015)
                     /locus_tag="IWL52_000686"
     CDS             complement(30832..33015)
                     /locus_tag="IWL52_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9777272.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33012..34037)
                     /locus_tag="IWL52_000687"
     CDS             complement(33012..34037)
                     /locus_tag="IWL52_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9777273.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34082..34909)
                     /locus_tag="IWL52_000688"
     CDS             complement(34082..34909)
                     /locus_tag="IWL52_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9777274.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(34906..35973)
                     /locus_tag="IWL52_000689"
     CDS             complement(34906..35973)
                     /locus_tag="IWL52_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9777275.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(35999..36835)
                     /locus_tag="IWL52_000690"
     CDS             complement(35999..36835)
                     /locus_tag="IWL52_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9777276.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(36832..37701)
                     /locus_tag="IWL52_000691"
     CDS             complement(36832..37701)
                     /locus_tag="IWL52_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9777277.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(37771..39054)
                     /locus_tag="IWL52_000692"
     CDS             complement(37771..39054)
                     /locus_tag="IWL52_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9777278.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39068..40753)
                     /locus_tag="IWL52_000693"
     CDS             complement(39068..40753)
                     /locus_tag="IWL52_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9777279.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41028..42053
                     /locus_tag="IWL52_000694"
     CDS             41028..42053
                     /locus_tag="IWL52_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9777280.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42648..46301)
                     /gene="rpoC"
                     /locus_tag="IWL52_000695"
     CDS             complement(42648..46301)
                     /gene="rpoC"
                     /locus_tag="IWL52_000695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9777281.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(46380..49997)
                     /gene="rpoB"
                     /locus_tag="IWL52_000696"
     CDS             complement(46380..49997)
                     /gene="rpoB"
                     /locus_tag="IWL52_000696"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9777282.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            50725..51693
                     /locus_tag="IWL52_000697"
     CDS             50725..51693
                     /locus_tag="IWL52_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9777283.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            51711..52616
                     /locus_tag="IWL52_000698"
     CDS             51711..52616
                     /locus_tag="IWL52_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777284.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52613..53419
                     /locus_tag="IWL52_000699"
     CDS             52613..53419
                     /locus_tag="IWL52_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9777285.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53576..54544
                     /locus_tag="IWL52_000700"
     CDS             53576..54544
                     /locus_tag="IWL52_000700"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9777286.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(54861..55385)
                     /locus_tag="IWL52_000701"
     CDS             complement(54861..55385)
                     /locus_tag="IWL52_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9777287.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55672..57426
                     /gene="scm"
                     /locus_tag="IWL52_000702"
     CDS             55672..57426
                     /gene="scm"
                     /locus_tag="IWL52_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9777288.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
BASE COUNT        16339 a        12562 c        10496 g        18042 t
ORIGIN      
        1 caataatacc tgttaaagca ccttcttgta tttttaaaga aatctcgttc aatgctaaaa
       61 aatcgttata acaaacatta agctttttca cttctaacat aactttctcc ttctaaatga
      121 taatgattat cacgatcata tggtatacaa aaattatatt ttagtcaacc ctaaatttta
      181 taatttagat ttttttctgt tgttgaagat taggatgtca aaattatttg aaactactcg
      241 tatagtaaat agtaagtaat tttcaaaaca cgaagaaaaa caaaaagaaa aaaggaaacg
      301 ccgaagcatt tcctttcagt gatttaatga gaacaacacg atttgactta gagtctcaaa
      361 aagtcgaata ggtacactca tttaagtgtc tccattcgac tttttgggga tgagctggct
      421 attgaggtcc gcttacccta ttgaataaat acaagattgg gtatttctaa taagtccgcc
      481 cttcatattg gggtgatcgg ggcgaacatg ccatcttttc atataccacc tcctcgacac
      541 tcaataatca aagattctcg ttaatcttct tcgatgcttg caatgatgtt ttcaatattc
      601 atataacgaa aatcttcttg tttgatataa acgatctgag cggtacattt ttcctttttg
      661 atgatttctt ttactttttc ctcagaacgt gcgacctgcg gcgttaaaag taaaacgtca
      721 tagtctttat aattcagttt ttcttcaacg tttcggatcg ttgcataatc tatctcatac
      781 gatagatttt tggctctgat cgtttcaagc atttttgata cgagcagcga cgcactgatt
      841 ccggtctcgc aaataatcaa tagatcctta tgcataatac cctcctgttt ttagcctgca
      901 aagccaattg gtgaaaatgc cacataagtt aaaataacaa tgactaccaa tgctatagca
      961 gtcaccttgg tgtatttcca aggctcgata tcgacctttg tatcgaatga gttcaacaca
     1021 aattctccat caggtttcaa tttggaaacc accatcaaaa tgataatgtc cagaacgaat
     1081 aatgccgtca aaacataaag gaaatggata ttttggaaaa gcatttttga tagtgcatac
     1141 aagacgatat gcacggcaat cgtcgttttt gccccaaaag cagttgcttt ttttgaataa
     1201 aaggcaaaga gaatgatcac gagcagcggc atgttgtata gtccattgaa ttcttgaacg
     1261 aactgataca atccagccgg tgcaaaagaa atgaatggcg ctacgataat ggagataatc
     1321 cccacaacga ccgttactgt tttaccagca cgagcgattt gcttatcatt tgctttctta
     1381 ttgataagcg gtttataaaa atccagtgaa aacaaggtcg atgtcgcgtt caaagctccc
     1441 gcaaatgatg ataagatcgc gccaaagata acagcggcga aaataccaaa gatcatttta
     1501 ggcaatactt cactgactaa aaaaggatac gcgttatctg gatttgacat taaactatcg
     1561 ccaaaaatat tgcgagcgac gatccctgga aagacaagga ataacgcacc taagattttg
     1621 aagacgccga ctaggaaagc gcccttttgt gcctcagcta aattcttgcc ggacaatgct
     1681 ttttggacga tcatttgatt ggtacaccag aaatacagat tgttaaacag catccctgta
     1741 aatagtgtcg gccatggaac aacagccgag tcgatcgatc ccacagagtt aagcagccat
     1801 ggcgtgttta ccacaacgtg ttcgatcccg ccaacaaagc tgccgtcacc aagcttcatt
     1861 aagcctaaaa ttgggaccag cagaccacaa gccagcagac cgactccata aatggagtca
     1921 ctattggcac ttaacgacaa gccgccgatc agcagataga ggatgccgac aataccaatt
     1981 gccatcgcta caattatgat cgcgacgatc ggctggacgc ccagcagctc atcaatatgg
     2041 aagatcttat tgaacaccag cgatccagaa taaagaacca cgggtaagaa agaagtcata
     2101 taggtcacaa taaataagat cgaaataatt cgtttggtaa ccgtatcata acgaatctcg
     2161 atgaaatctg agattgtatt gatgccgtat ttaaaatatt ttggcaagaa tatcacagcc
     2221 aaagctacga tcgcaatcgt agcagttact tcccaggcca ttacttccat ccctgcatga
     2281 taactttgac cattttgtcc gactatttgt tcggtggaca ggttcgtcat gatgatcgtg
     2341 ccggcgattg gcaaagcagt taagctccgc cctcccatga agaacccttc tgatccagaa
     2401 gtatcgacac ttctggatct agaataggca aataaccata cacagatgac aatcactagg
     2461 aaactgatta tagaaaaaag attcatcgtc tagctccttt tcaatagatt aaagaccggt
     2521 gacctctcga atgtagcttc tggctttgac tgcatattca aatggattgg ctttagccgg
     2581 gtcctgttct gcttcaacta cgatccagcc ttcatagtcg ctggcatgga ttgctgtcca
     2641 gattggagtg aaatcaatca tcccatcacc aggaaccgtg aagacgcctt tcttcacgcc
     2701 gtttaagaag ctgtcttttt ccttttctac ttcttcagcc gtttcttgac gaatatcttt
     2761 gaaatggatg tgtttgatac gatcttgatg acggttgtaa accgcgattg gattttcacc
     2821 agaaaagacc aaatgtcccg tatcgaacaa taatttaact ttgtctggat cagtcatttc
     2881 cattaaacga tcgatttctt ccgtcgtttg aacgccggta cccatgtgat gatggtaaac
     2941 gatctccata tccttttcgt gggccaaatc ccctaatttt tctaatcctt ccgccagttt
     3001 tttccactct tcatcagtga aaactggttt atcatcaaag actggcgctg tcataatccc
     3061 ttggatacta tgaccctgct cagctactac gatgacttta gcgcccatcg catgtaagaa
     3121 atcccgatgt gctttgaagg cttcggcagt ttcttcaaaa ggtttagttg tcagaaacgc
     3181 actgaaccat gcactggcaa cctttaagcc gcgaatattc aaatattgtt ccaacacctc
     3241 tggatctttt ggatatttgt tgccaatctc tgttccggtg aacccagcaa gggccatttc
     3301 actgatacat tgttcaaagg tattttcact accaagctcg ggcatatcat cattggtcca
     3361 cgcgattggt gcgatggcta attgaatttg tttggtcata ttctcacact cctgcttatt
     3421 tttttagttg tactaaactc ttcgtatcga atgattcttg acaagccaac gcgatcttag
     3481 ttgattgcaa tccatcttct gcattaaccg ctggttgacg tttttcttga atacatgaga
     3541 caaattcttg ggcttcacga atgaaggctt ctctgaatcg ttctgggaag ttttgtgaac
     3601 atggacggat gatcccttga tcgttcatga tcgtcacaag atttttctca ggcgcttgcg
     3661 ctacccgcag cattcctttg gttcccatga tctctgtttc gacatgatag ccatgggctg
     3721 cgtttctgcc ggcaactagt aaagctattg ttttatcgct caggctcatc attgcggctc
     3781 cagtttcaag ctcaccagcc tgatccaatt ctggatacgc cgcatttttc ccaatcgccc
     3841 acacttgatc gacttcttgg ccggtaaacc agcgaaccaa atcaatatca tggatcgcca
     3901 tatctaagaa caagccgccg ctgtcgttat tcatggcaaa tttgacaaag ctttccaagc
     3961 cttcgctcgg atcgatccca taacaacgaa tcagtgtgat ttcgccgatt tcgccatctt
     4021 ctaccatttt cttcgcatac tgatacgact catcatagcg gcgcatgaag cctaacatga
     4081 agacctgttc aggatgctct tcgatgactt tttgggtctt tttaatatct tcaacttcaa
     4141 ggcctaatgg tttttcagta aagacatgct tgccattttc catcgccaag cggacttggt
     4201 cacagtgaaa gcctgatggt gaaacaataa agactgcatc gatcacggaa cttttgacca
     4261 tttcttcata gctggaaaaa gtatgcttga cgcctaattt cttctgtgcg aatgctaatt
     4321 ctgtttcaac taaactgcac gcagccgtaa gctcacaaca actgacacct gttgctaaat
     4381 tttgtgcatg tacttttcct aatcttccta aaccgacgat gcctactcta atgttgttca
     4441 tgaaatacct ccgttttctg agaattactc attgtatagt gctggtcgtt catccatttg
     4501 aatcattact tttgtttttg tttcacgaga ctcatgacaa gccatcatgg tgatcgctgc
     4561 tacataccca tcccaagcgg taggaccgac aaccgcatca tttttgatcg aatcgatcca
     4621 cagcttgaat tcataatcat aggcatccaa aaagcgggtc tgccaatctg gtgaaacagg
     4681 tgtgtaattt ttttcttccg atttaattcg tgtataggct gggtccgtca ttcctacttc
     4741 tgccgtttcg aaaaccacat cacatttaat atcgtagcca aagcggcaat tcacaaagat
     4801 ttccaaatca atcgttacac cggattttgt ttgaaagatc agcagctgcg gatcatgcaa
     4861 atcggcatgg gagttgctgg tttttttcgg caagatcatt tgaacagatt cataatcttc
     4921 atttagcaac cagcgaagca catcaatctc atgaatcgcg gtgttgctga tcgccatcgg
     4981 tgttgtgtag ttttcatctg cgctaggatt tctatgggca caatgcatca ttaatgcttc
     5041 tccgtattgc ttcgattcga ttgcttcttt cagttcaatg tagccgcggt catagcggcg
     5101 catgaagcca acctgaacca atttcttccc ataagcaact tccgcatcaa cgatccggcg
     5161 gcagcctgct gcatccatcg ctaatggctt ttcgcagaaa acatatttcc catgcttgat
     5221 tgcttctaac acatactgct catgggtcgg gtcccaagaa gtcacaacaa cgacatccac
     5281 atcttcagaa gcgatcaatg cttctccggt ttcaaagaat ttagcatcga cacgtttggc
     5341 aacctcttta gcagctttta cattgatatc tgagactgct gtcacataag ccccttgtgt
     5401 tttctgattg atcctgtcga tatgctcgat gccgattgca cctgttccga taacgcctac
     5461 ttttactctc aattacttat ccccctgttc tgttgtttaa gcacacatag tatgcgctta
     5521 caaaattatt cctgtgtagc ttttattctt aaaaggtaga gccctctctt tgctccgcct
     5581 tttactaccc ataagcttgt acgtataatt ccttaatttg actatgacta ggcacctttt
     5641 tattattttg cggacttccg ctggtcaacg catccgcggc cattttgtct aaggctgcca
     5701 taaacgcttg ctgatcaata ccataatctc gaatcgttgg gatcgacagc tgctcacaga
     5761 gctcttggat ttttttaatc agataatccg cagcctcttc cagcgataaa tcttcggctg
     5821 ccggataaat aaatctggcg atcgtcgcta actcagtctg aatagcatcc tttgagaatt
     5881 caaaaaagac cggcaataac atcgcatttg atatcccatg aggaatgtga aaaagtgccc
     5941 ccaccggtcg actcattcca tgaatcaatg tgactgacgc attcgaaaag gcaatccccg
     6001 cctccgttga agctaagctc atttgttctc tggcttcagt gtcgtcgcta ttttcatatg
     6061 cctgagaaag cgtgttcaaa attttcttca tcgcttccag tgaaaatagt tgcgttactt
     6121 cattggcttt gactgaaaca taagattcca aagcgtgaca tagcgcatcg actcccgttg
     6181 ccgcggttac ttttggtggt acagttttcg taaaatcaga gtcaacgatt gagatctgtg
     6241 gcatgaaaac gagatccttc atcatcattt tgactgctgt ttttgtattg gtaataactg
     6301 ccacatcggt tacttccgaa ccagttccag cagttgttga cactgcaatc agcggaatgc
     6361 ccgcttgcag ctgatcaaaa tttcttgcat gaatcgcttc gagacttaat tgattagcag
     6421 ccaaaacggc taccaccttg gctgtatcca gacagctgcc gccaccaaaa gcaatgatat
     6481 gatcacattg atgcttctga aaaagcttca gcgcatcata aacattttct tgcgtcggtt
     6541 cggcttgaat gtctgtatac tccgctaatt gatcatctgt ttttcccaac agctccttga
     6601 tcttttgaag atgcccggtc tgttccataa ccgtatcact aatgattaac ggcttgttgc
     6661 ctagttgttc tgattcttcc ataagaaatt ggattgttcc gccgccaatt cttaaattgg
     6721 caggcatttt cagttgaata ttcacagaaa aaacctctct ctggcttata gcctataaca
     6781 gaatcctcga agctctttaa cagagcttcg aagcaaatct ttaaatctca ctttgcgcat
     6841 aacgtgctgt aacaactttc cgatgggtat aaaactcaac actgtctttg ccattggcat
     6901 gcaaatcacc aaagaaagag tgcttccagc ctgaaaaggc aaaccaagcc atcggtgcag
     6961 gcaccccaag attgaccccc agcatcccag catcaacatt ttctctgaag tagcgaatcg
     7021 ctgatgcgtt gttggtatac agacacgaac cattcgcaaa ttcagattca ttcgtcagct
     7081 caacggctgc catcaaatct tttacccgca cgattgatgc gaccggggca aaaatctcat
     7141 cctgccagat cttcatatcc atcgtgacat tatcaaaaat cgttggtcca acaaaatagc
     7201 ctttttctgg aatgttttcg cgaccatcta gtaccagttt cgctccttca tcgacccctt
     7261 tttggatata gtcaaaggtc cgctgctgtg catcttttcg aatcactggt cctaaaaaga
     7321 ccccttcgtc caagccattg ccgatcttga tcgcttttgc cgcagcctta aatttttcaa
     7381 caaactgatc cgcgatttcc tcttccaaaa caatgacgga ggctgccatg cagcgttcac
     7441 cagcagaacc aaaagctccg ccaataaggt ccttgactgc ccgatcaata ttggcatctt
     7501 tcagcacaac aacatgattt ttcgctccag tcaacgcttg tacccgtttt agattttttg
     7561 ttccttcacg gtaaacgtat tcaccgacat tttttgatcc tacaaagctg atgcctttta
     7621 ccagtggatc ttgcagcaat gtgttgacaa catcgtgggc accatagaca acattaaaaa
     7681 caccgctagg aaagcctgct ttttcaacca gctcaacaat tttctccatc aacaatggtg
     7741 ttttttctga aggcttcagc accaccgtat ttcctgccgc cagagccatc gggaagatcc
     7801 agaagggcac catcattgga aaattaaacg gtgtgatccc tccgattact ccgattgggt
     7861 aacgataatt cgtgatctca atatcagtcg ccgctgttga taaagaatct cccatcatta
     7921 aattggtgat tcccgctgcg tgctccacat tttcgattcc tcgttgaact tctcccagtg
     7981 actcggacaa tgatttccca ttttccagcg tgatcaaacg cgccaattca cttttatgat
     8041 ctgttaaaag ctgatgcagc ttgaacagat aacgtccgcg aacaggaaca ggaacttttt
     8101 tccaagtttg aaacgcagca tcggcagctt taactgcttc ggctgtctct gctttgctag
     8161 acaacggtac attcgccagc gcttcttctg tagccggatt cattacttct tcaaactctg
     8221 ttgagcgact agcgacccaa cgaccatcaa tataatttct taaaaatctt gtcatttatt
     8281 ttcctcctaa aaacctaact aaacctaact aaactttatg tatctaacag cttaataact
     8341 acttgcgctg caagatccat tcgtgtttct tttcattgtg gaatttccaa attcgctcag
     8401 gtcccgccat cacatttaaa tagtaggaag tatacccgtc aggtacccca actggatgat
     8461 agcccttagg aacttcgacg acattttgat gctctaccgt catcgtttca tctagtgaac
     8521 gatcttccgt gtagacccgc tggaaaacaa atccctgctc tggatcaagc tcatggtaat
     8581 aagtttcctc taaaaaagac tcatgtggca gattctcttg atcgtgctta tgaggaggat
     8641 agctggaaaa attaccgccc tcggtataaa cttcgacgac cagcaggctg ctggcagtat
     8701 cgacattatc cggtaaaatg ttgtgaacaa gtcgtttatt ctgatagagt ccacgttctt
     8761 cgacggtatt atcctccgcg cgaatcacac ttatcggcaa agcttttttc gctggtgaat
     8821 agcaaagcgc caatcggaca ttgtctgtag ttgccacaag ttcaaacgaa tgctccgccg
     8881 gaagataaat actgtcggtt ggaatttttt caaaaacaga ttctcgtgtt cctaaatctt
     8941 cataccaagt gtctgctgct ttaaccttaa tttttccagt caaaatgacg atacagacct
     9001 cctgttgact gccggtttcc ttgtaagaag ctcccttttc cagctccaaa atctgaaatt
     9061 cgatataact caaagcagaa ttagactgcc gaatattttg gatttgtttt actccaggtg
     9121 ccagttcttt gtttaatggt tgatgcttca atgtcttttc caatcaatcc actccttatt
     9181 ttcaatcaca aaaagcaata tttaatactt tctgatagtg gcaagtttcg cttgtttggc
     9241 tttgtaagcc tcctgcatgg ctgctttacc cgatacctct gagataccga cgttccacca
     9301 gctgccgtcg tagccttctg tcatggtttt aggaagtacc tttatctcaa tcaacgtcga
     9361 tcgcgtttgc ttcttcgcat cctcgaccgc tgcttgtaat tctgcaatcg agcttacacg
     9421 atacgttttt gcaccatagc cttcagcaac tttggcgtaa tcgatgttca tgatctcgtc
     9481 cttggtattt ctgaattcgg ttccttggct agctccgcca ttatccatct gtaaattatt
     9541 gatacagccg aaaccagaat tatcgaaaag tacaatgttg atttttttat catattgcaa
     9601 actagaaacc agctcgctat gcaacatcaa gaaactgcca tcgcctacta aggcatagac
     9661 ctcttgttct ggtcgagcca ttcttacgcc catcgcacca gcgatttcat agcccataca
     9721 agaataaccg tattccaaat gataggtatt ctccttcaca gggttccata agcgctgcat
     9781 atcccccggc aatgaaccgg ctgaaccgat cacgatcgca tcctcatcaa caaaatcatt
     9841 taaatgaatg aagacctctg tttgcgtcaa attcgtagcc aacgcatcag catattcatt
     9901 caagatttct tgagaaaaat gatctttgat ttctggcgta aaatctttac gcttaaaatg
     9961 cgcggctttt aaccgatttc gctcactgat ccattcttcc ttcagttcat cgatcttctc
    10021 gccataggcg gtttggtaac cttcgatcat tggggcaagc tgctccaagg tcgctttcgc
    10081 atcaccaacc acttgaaagg cttccagctt agcggcctgt tgtctgctta cgttaatatt
    10141 catcattttc gcttgttcaa aattaaaaat acttttagaa gctgaggtaa aatcagtgta
    10201 gcgtgtgccg atccctatga tcagatccgc ttgctgcaca gctttattag cagccgaagt
    10261 tcccaaaatc cccattccgc cgagattgtt tttaaaatcc cacgaaacag tcgatttacc
    10321 tgcgtgcgtt tcaactagcg gaatctgaca atctcttgag atttgctcaa gaatctcacc
    10381 agcctccgaa tatttgcagc cgcctcctac gatgatgaca ggtcgtttac tggcctttat
    10441 tcgttgactc gcttcagcaa gctcacgagc ggtcggaatc ctgcgatcaa tataatgtac
    10501 tctttttttg aaaaattcct ccggataggc ataggcttct gcctccacgt cctgcggcaa
    10561 acaaatcgtt gccggaccca tcgttgccgg gttcgtcatg acttcaaagg cccgcaataa
    10621 cgcggacatc aactgctctg gtcgattgat gcggtcccag tagcgagaaa ccgcttgaaa
    10681 tgcatcattt gtcgaaaagg ctacactgct tgcatgttcc agctgctgca agaccggatc
    10741 aggctgacgt gtagcaaacg tatccgccgg taaaaaaagt acggggatat tatttgcgaa
    10801 tgccgtaccc gctgctgtta acagattggc cgcgcccggt ccaacagatg ttgatacggc
    10861 aaaaattttc ttgcgtaaat tctgtctggc aaaggcagta gccgcatggg ccattccctg
    10921 ctcatttttt ccgctataac ttttcaaatg tccgggctct aattccagcg cctgaccgat
    10981 ccccaaaaca tttccgtgac catagacatg aaagatacct tcaacataag gcgtttcttt
    11041 gccgtcgatg ctgatgtatt gttgattcaa aaattttagt aatgcttgtg ctgttgttaa
    11101 acaaaccgtc ttttccattt ttttcacctt aaatccgagc gatcatttcg ccgtatattt
    11161 ctttctccaa tgtaataaaa tcttttacct cctgagtcgt cggtaaagaa tctgaacagt
    11221 tattgctttt gaccatcatt gatgcttccg ctgagccaaa ctctaaacaa tcgatgatct
    11281 cccagccttc gaataaaccg tataagaaac ccgagccgta accatctccg ccgccaaagc
    11341 ctttgcgtgc ttccaccgga aacggcttga ttgaaaactg ctgctgatcg gccgtataag
    11401 cagtcgatcc tttcattcca tgtttgatta cgacaatctc agcaccacaa ctgtgccaaa
    11461 aagcagcgct ttcattatct gacatgttag gctggatcaa cttctcagtt agatcgaact
    11521 cttctcttga acccatgata atgtctgatt ccttagcaat catcgaatag taaattgaaa
    11581 tatcatcgtt gttcttccag ttataagcac ggtaatcaat atcaaaaata attttgacat
    11641 tattcctttt cgccagcatc actgccttga ttacggcttc tctggacggg ctctctgcta
    11701 gtgcagttcc tgagattaat agtgcttttg accgtttcaa gtagtcctca tcaatatcct
    11761 caacgctaag ctgcaggtct gcggcttgat ttcgatacat caaaatatta ctttcctttg
    11821 atgagagcat ttcggtaaaa gtcagcccca gcttttcacc atgctgcgct ttgctgatct
    11881 gagacgtatc gatgccttgt tcattgaaat aatcgatcac atattcgcca aactgatcgt
    11941 ccgagactgt tccgataaaa ccagctttca tgccatgttt agtaacaccc acagcaatat
    12001 tggcaggaga tccgcccaca aatttttcaa acatatggac ctttttcaat ggtttaaact
    12061 cttctttgac ttgatcattg taggcaggat taaaatcaat agcgactctt cctaaaagca
    12121 gcaagtcaaa ttcttttgat tggtcaaact ttatataact caatatatcc acctctttat
    12181 ccgttcttct atttctgagg caagtatagc acgccaaaac aaagcgtgca atatttattt
    12241 tagaacgcaa tattgcgtgc tgtttgaaaa tgacggcaaa aaaaacgtga atcgccttac
    12301 atcacgattc acgcctagat taaatattgt attttgtctt aataattata tcagtaaata
    12361 gcgcatcttt tgtaaccgtt ttattatttt gcagctgatt cgctaacatc agtaatgaac
    12421 gatagccttg aacaaatcca tcttgatcga ttagaaaatc aacctgatcg tttttcagca
    12481 tctcgatatt gttcttagtc aaatcataaa tgattacaaa gggccgcttg ctttcatcga
    12541 ttttttccaa cgctcgggca attcccgctt gtccgccaga aaagacgact acgccatcta
    12601 agtttgaata attctccaag gtgttgctta aaatcttttc aacctcagca ctcgaatcaa
    12661 agctgctctg aacacccgtc agctccagtt caggaaacga ctctttcaat tcttcaacaa
    12721 agcctgccac ccgtaagctg ttgacgctat ttccaaagta gccggtaatc gctagaacct
    12781 tgccctttcc ttttgtcagc atacctaaca atcccgcagc cgtttttccg cttttgaagt
    12841 tgtccatccc aacaaaacac tgtcgattcg taccaagaat atccgaatta aaggtaatga
    12901 tgaggatgcc ttgatcggtc aattgattaa ttttttctct gataccatca ctatctactg
    12961 gcatgatcgc gatccctttt actccaagct ccaccaatcg atccaacgcc tgcagctggg
    13021 ccgcttcatc gaccccgtct acctcttcta aaaaacaatc aatgtttcgc ttttttaaat
    13081 ccccgataat agattccaga cctgatcgga tcggaatcat aaaagaagat ttcgccaatt
    13141 gagtgacaat ccccagcttg agcgttgtct cttttatgac cttctttttc tttggaacat
    13201 agttcatttc cttggcgatt gctctgattc tttctgcaac ctcaggattg atccttcctc
    13261 gatcgttcaa tgcccgatcc actgtccccc ttgacactcc tgctttctca gctatttctt
    13321 taattgtacc cgccatcttg cctctccttt tttcctattg tgtttatttt atcacaaaac
    13381 ttaagaaaac caccggagaa tgaattctcc ggtggacttt ttgtatgatt aaccttctta
    13441 ttgaacatca ctgatcttaa actctattcc aagggcgttt ctataatatt ctggatacat
    13501 ctccttccca catttttcac aggtaaattg gggaggattg ttcggatcat ttgcaatatc
    13561 tgcatcatct aaaaatttta ctacatccat tgggattgat tctttttcac cacaacttag
    13621 acatataaat ttcacgttgg cgttcccaat cccagcgaat tgctttaaaa gttttttctt
    13681 tttcttcttc atactgttgc ctgaattctt tttggtcaat ttttcgattc tcctctcgca
    13741 taaaccgctt tgcatggtta tctttcgcat atttaataac taaccgtcca tttttgcgca
    13801 cactcatccc aacgaattca tagtactcac cgcaacaaga acattctaag ggattttcac
    13861 caaattcttc ggttattctt tcttcccagg attttggtct taaggcctca ttcagattga
    13921 taaacagctt ttttactcgt ttctgatact ccttcatgac ctttttcatt aatgttttga
    13981 ttctacgact atagattcca tagcgacgaa tcgttttgaa attcttattt tgaatatggc
    14041 gaatcagtgc agctatgaat tcgtctatac tcatggtcat cgtctcttta gtattcgttc
    14101 gtttgtccat gtagttgaac atgacagtgt cgccatcata cattaagatt cggcttaatg
    14161 cgatcgggcc acgcttcata taacgactga tgtattggag tagtgacttt agatttgtcc
    14221 tgctcctttt aggagcattt acgtaaaacc cttctccatt gttcttgtac gcagcttgta
    14281 atcttgattg aactctttgc ttgtcccact cacctaacgt tcgtctaatt agtttcaata
    14341 cagcattttt ccagtatttt cttaacatag aaaaaggtaa ataatcgtat tcttcccaac
    14401 ttccatctct tgttaagcca cccatagtaa caaccatatg aacatgcgga ttaaattcta
    14461 gttttgatcc aaaggtgtgg attgtagcta ctatacctgc cttcaccttt cttttctcaa
    14521 aataggcaag aataattttt gcggcttcat ccattagtcc tttcagtaag gaatctcgat
    14581 agttttccat cataaagatt tcccgtaacc cctcatcgat cgtaaagatt acgtgccgat
    14641 gtgtcacttt aaacatatca ttcgccacaa ccgctgacca cctttggctt tcacctgttg
    14701 cacacgttgg acagaacttt ccacgacatt tcaagggaac aaactttgtt tgatgacatc
    14761 cctcgcagac aaacagtcgg tatccttttt gaatatctcc acaatatcta aacttgttta
    14821 cttcccgttt gacgactagc cgaatctttc gcccgtattt ctcaacaaag ttatcccaat
    14881 tatggtgttc gtcaaaaaag attgcttcga gaatattcgc tttcatagta atattttact
    14941 tgttcaaaaa caaaatgaaa agggctaaaa gactggtagt gcagcctttt tagcccttaa
    15001 ctttttatac tttctttact gtctaaaaaa tatgcggcgc acagaacgcg ccgcatatcg
    15061 gactaagttt tattcaaatt tgtcttcgcc aacacaattt gacgaagagc cttttctttc
    15121 aggaagactt cggtaatttt taggagttct acctattctt ttaaccgttt attctctttt
    15181 tctaagcgtg taatttcatt tctgcgaatg gatttcatga cttcctctac cgagaaaagt
    15241 acgttatcca tatttttcaa cttcttgaac tcatcaatac acgctattag agcgagcttc
    15301 tagctataga ctaacttctt taacagaaaa cccttcttcc gttaccagtt ttacagcggt
    15361 aattttaaat atttcatcaa atgaacgtct tggcataatc cagatcttcg ctttctctta
    15421 atttggtgtc aattttagta taacccgtcc aacttatttt ttaactaagt gcccggtttt
    15481 tcttgacaac tccacccaaa agttagattt tttggtctaa gttttagggt cgcttcatta
    15541 ctagaattca acaagagatg ttttttattt tatttaaatt atggggtcag tttccatttg
    15601 attacaaact tctttgtttt ctttttttaa acgcaataat aaaggtttta atttcagcct
    15661 ttttaattag aaaatcaatt ttcccaaatt attttcttta gctttcttaa cgatttctgt
    15721 cgcaactacc acatctaata cagccgaacc aactgttttg aagatcgtga tttgttctcc
    15781 acttgtccga ccgatcaatt cttcattgat cagttggcct agttccccat gatagctgtc
    15841 tctttgaata tacccatctt gcagcggtga aatgaagtcc ccagcttctt ttagaacacc
    15901 gtccatggta tcaaaaataa caacatctgc ggctttgatt atttctcgtg gaatttcaca
    15961 catttccggt gtgtaagcac cgactccatt gatgtgcgca ccttttttga cccactcagc
    16021 agagaatgtc gcacgttttg aagtggtcac acttgtaatg atgtctgctc cttcaacgca
    16081 ttcttgactg gtttttgttg ggcgcatcgt tacagaaaat tgttgcatca ttttttcagc
    16141 aaattggctg gcacgctcaa aatcaatatc aaaaatccga acttcttcta atttacgaac
    16201 ggtcaacatt gcttctaatt gtgactgtgc ttgtccgccg gttccaatca gcgcgccaat
    16261 tttagcatct tctcttgcaa gaagttctgt tgctgctcct tgaacagcgc ctgttcttaa
    16321 ttgtgtcaaa taagtaccat ccaaacaagc ggataccata cccgtttctg gatccaatac
    16381 gatcatcgtt gctggtacac ttggtaagtt tttcttgatg ttttcaggat acacggaaac
    16441 gattttgaca cctagcgcgc cttcgccacc ttctacataa gcaggcatat ataagctttg
    16501 tccgttgctt tttggcacat cgatattggt gcgaagaggc acacttgcct ttcctgctga
    16561 atacaatgac agtgcttttt tgtccgcatc gatggcttct ctcattgaaa aacttttgat
    16621 aatatcttct tttttaagaa ataacatttg aatcgctcct taattagctg ctgtaacttt
    16681 ttttaattga ctgcccaaca tcgtatatgc actgtatagt aagaccaaaa tcacgatcca
    16741 tttcaggata taggtatcta aagatttgac taggaataca gcgaccaata cccccaatac
    16801 accgaaggtt gaagtaaaaa gtgtgatttt tctgctgtat tcattaaatt taatgaattg
    16861 aacactgccg atcggtactg agaaggtaca agcccccatc ataataggaa aggctgcaac
    16921 tggattcaag cctagcaagt agacagttac catcgttaac gcataagaac caatgccgat
    16981 attgttcaat gcaccataga caaagagtaa aaatcctgcc aagattaatt ttccgccata
    17041 caattcactg gctgttccgt ttgatggaat ccagttcatt tgtcctgcaa aaatcaacaa
    17101 tgcagcaatg accaatccta tgccgacaaa cagtttgatc gttttctctg gtaacttcac
    17161 gacaaatctt ggtcctaaat atgcaccgat aatttggcag ataatacaaa cagctagtgt
    17221 cttgatccct acgttgatgg cagtgatgta agacaatgcc atgacggcta ccggtactac
    17281 acattgggta ttcaacgttc cgggtagttt tttcatgctg acccatttca actttggata
    17341 aaggacggtt ccgatagcaa agtcggaaac gccaaatgtt gaaagaaaga agatgataaa
    17401 tgacgtgaag ggcaacagac gcatatcccc tggttcttct ttgaaatttt gtttgttttt
    17461 gaaaaggtct cttagaaagt aaataataaa atatagattg attgcgacaa ttagtgtcag
    17521 caaaatattc cgaatcataa aaaattcctc ctactagtaa atctttctga ttccctcaaa
    17581 gccctcacca tattttgctt taagttcttc aggagattct gcaatcactt ttagtgtata
    17641 tgcatcatgt ttgacatatt cttttgctag attgaccatg gcgtggtttt ttgtccaagt
    17701 gacatcggtt gattgcagtt cttcgttaat ggtaccaaac acgacttctt gctcatcggc
    17761 gacgatgttg atccatcttg ctccaaagtc agcaagtttg tctgtttcaa aaccatgaat
    17821 ataatacttt tctaagggaa gatcatattc atgggtagaa ctgaataaaa tcagtacaaa
    17881 cttatcaact cttttttcag cttgctgcaa tgcctttagt agtgaatccg tgaaattctc
    17941 atgccaaact tgaatgagca aactttcttt tgcgtttttc accaaatgct ctactttatc
    18001 cagaacatac tcgataccat caactttcca cagcatttct tcttcatctt tttctttgat
    18061 catccctaga ttcttggtaa gaaaagaaag atcattttta acactttttt ctaatttctc
    18121 taaaaactca tttgccgaga tggcatgata aagtttaggt tctgatttat taacgacaat
    18181 caaattcttt tttaatagtt tttccaaaat attatatact ttcgagcgtg gcacacctga
    18241 gattttactt acttcatatc ctgtaagatt acctttttct aatagtgtgg tatacactct
    18301 tgtctccatc tctgaaaaat catatttttt catgatttga ataatcgttt ccattgatta
    18361 atcctcactt tatttttagt agctactacg gtaacaacta taaattcttt ttgattatct
    18421 gtcaagagtt agttcaataa cacacatata ataattagct taaaaacaat ctttttatta
    18481 aaaagaaaca ttataatatg aatgtttgtg aaaaaataat acaacttagt cattctttac
    18541 aacgatttat agaaaaacaa cctctttaaa tataaaaggt acgggttact gattacttgt
    18601 aaatctttac gttccagttt tattctttct aaaaaatata ctcacttctt ttcttagact
    18661 tctttttttg attctcctct ttatggtctt gtcctttttt gccttcaaaa tatttaaata
    18721 aacccctttc aaaagcattt ctaggttttg gacaataagg aatgactttt ttcttcatat
    18781 aaatacctca tttaactatt tgatatcatc acattatcaa aaaaaatagg ttttttatta
    18841 aaactatctt gacctttcgg gagatttcac tgtgatttat ttttattccc atactttttt
    18901 cgttggtcac tttcgtatac ctaatctaag cgatcatcgg cgacggtgct tttacaacga
    18961 atggcaggac aaaacaaggg gcggaaaaac ttttttgcct atttcataaa aaactttttc
    19021 ctacagcaat cccttgtttt gtcctaccac acgttgtgaa cccaaagcac gccgacgaac
    19081 gcttagctat acgaaaatga cctccaaaaa caaaaaagtg aggtgaatag aaacaaaaag
    19141 aaattgaaat ctcccaaaat gtcgtccgcc tcggagacaa cttgctgctg aaaatggttg
    19201 gttaattcat ttaagtatta aataagataa atcatcaaat taatcggaaa aatagcatga
    19261 ttggccttat caaaaataaa ctgtaaaaac ttatatttct atgagtggtg gaacaatgct
    19321 aataccaaat taatttatcg attacccacc tgcacaaacc aaagatagca ttattaggaa
    19381 acgaatcaat cagcgaatcc agcattataa tgaggatact aaaaataaag ttttcataat
    19441 tataaatccg ccttcctacg aaaggcacca atgaggtgat gaaaacctta gagcgctttc
    19501 tttctttttt tattccgtgt taccctttcc ttgcaggtcc ctggtacact acagaacccg
    19561 aggaggtgag tggcggggct gtatagcggc aaccccgcat aattatatgg tgtcggtcat
    19621 ccgttaaggc atcaatgaat ggcgcaaacc cgtagcccgt aaacttatct cttccgaagt
    19681 tgactcgatt tcgccggatg accagtccct accaaacctg tactattttt ttacaggagg
    19741 ttgctatgtt caaaatcttt cgtaaaaatt gttgtggctt ggatgtccac aaaacgtgga
    19801 tctatgcgtg tatcggaatc acagatgcga atggtcgtac agtgtataag caagctcgct
    19861 tctcttcctt tacaaaaggt ctcaatgaat tagctgaatg gcttgctaaa tacaactgta
    19921 aggatgtgtg tttggaatct tccggtaaat tttggattcc tgtttttaat attcttgaaa
    19981 aaacttgttt tgtcacactg gctcatccga agtacaccaa accccagaaa ggaaataaaa
    20041 cagatcgcaa agatgcaaaa atggatttgt gatctcttta tgtgcgacat gatcaaaccc
    20101 agttttattc ctggtcttga aatccgccag cttcgtgatc taatgcgtta tcggacgaaa
    20161 ctgaccaaca tgaaaacaag tgaaaagaat cgtgctttaa actgcttaac cgtttctaat
    20221 ctgaaactag atgatgtttt tagtgatgtg ttcggaaaat catccacttc tatcatccaa
    20281 tacttgttag aacatcccgg tgaatcattt gatgttcgac catttatcca tgcccgttgt
    20341 aaaactcccg ttgaagaaat ccaagcggca gttgaggggg cgatttctgt acctcaagcg
    20401 attaagcttc gccaatgcct caaccatatt aacgagattg agaagcatcg agaagagatc
    20461 aatctagaga ttcaagctct tgcagaaccc ttctcccctg ttttggaact acttcgtacc
    20521 ataccgggct tagacactca gccaataaca gcgattgcga ttctttcaga aataggaccg
    20581 gatatgtccg tgtttccaac ttcgaaacac tttatttctt gggctggatg ctgtccaaga
    20641 aacgatcgca gtgcccgacg aacaaaatca acacgtatct ctcgtgcagg gcagtcgttg
    20701 aaaccgctac ttgtacagat tgccaatgct cttctcagat ccaaagaaca tccagaattt
    20761 aaaaatcgct atcgtagaat caaagcgaat cgtggacaca agaaagctat cattgctatt
    20821 tgtaagatgc tcctgaccgc tatctggaat gtcttatcaa aggtggaacc ctattctgct
    20881 aaaggatatc tggagccaaa acctgcgaaa gaagagaaag ttctgacgca atcccaagct
    20941 ctggaccttt taagaaaacg cggatatacc attatagata gtgcttaaac caaagttacg
    21001 ttgattcttc ctaaatagac tcgttgtctt ccatttcaaa aattttgaaa acggtaggct
    21061 tatttagtgt accgttcttc aggtatcttc aaatagattt attttcaaac ttatttcctt
    21121 tcttctttta ctctcctata gcttagcatt taagtgggca ctcgtgtcac cttgctagat
    21181 agctatgaat gccgtaataa gcctcctagt atataggcag actgccaatt ataactgatt
    21241 caatccacat tatgataaaa tccacctttt cgtttcatat gtgttgaaat atcacatatg
    21301 tcaaaagtgc acgagtcttt tcattcagtg ctttgtaaat actcaaaatt attccttttc
    21361 actttaaaaa aaagataatt agtacaaaat cagaaatgag gaaagtatta ggatggacta
    21421 gaaaggagtt gttgaatttg ttgcaacaga ttggtcattt atcaattaaa aattaggtga
    21481 agttttattc atcactgcag ctgctgtttc ccagagaata aaacgtctag aagaaactgg
    21541 tcagttactg tattatcaag cagcaattga ctacgaacaa gcttgattac taaccaaagc
    21601 gtttatccac ctgttgatta aatcaaatca aaaatcaggt ttttatacgt tcataaaagc
    21661 aatctccagt gtactcgaat gtgactgtat cataggtcct tattcgatgt taatcaaaat
    21721 ggggtttcct tctaccgaat cattggacag ttttatcaat gatttatcaa agtttggacg
    21781 aaccaacacc caaatcgttt tctcgactct tgtttcctta atggtttatc agttctggaa
    21841 tgtttatcat gcgcttttat taaaatagaa aagagctttt ttcatgcgac aaagcagtga
    21901 tcatttattg gcacatgcaa tctttgtgga tccatttttt gaaaatacga tatttagttt
    21961 tgacccaaaa atctttaaat acgtatttga taatttatct taacaaccag ataattttat
    22021 tcagcaccgt attatctata ttgaattctc ggaatcaaga taaatttttt tccgtttttg
    22081 tttctttttt aatcttcttc ttgttattaa tagtgattag gatgatattc ctatattgat
    22141 ctttgataat tagatataat ttttgaataa cgttccctgc tattcgagct agaaatacaa
    22201 gtacgccatg acctagttag cgacaactac ttagagattt cttttgttct gggacaacaa
    22261 aatgccatga caatagcagt aaataatgat acttccgtaa ggctcgccgt aaactgggga
    22321 gaggtgaaat tcctatgtgc ttacccaaag cggttcaaaa attttaaact attattttag
    22381 gagatgattt tataaaatcg aaagcctact tgaataaaaa acaagcctgt gaatttctag
    22441 gaattgataa taatacttta gataagtgga ttcgtgtata taattttcca gtaataaaaa
    22501 ttgatggcgt agttcgcttt gacgtagagg atatcaaaga tttcatgaat acattcaaaa
    22561 aaaattgaaa ttttcttctc gtcacatata ctataaatag cgaaatgtac tgccttactt
    22621 tttaaaggag tgaggaagtt tgcctacaaa gctttcaaat gggaaatata aaactaatct
    22681 gaggtatcca aaaaaattca gagaaatcac tggaattgca tcagagaagt atcaaaaagt
    22741 atttccaact aggcaattag ctattaaggc cgaaagggaa atgaaaaaga agattgaaac
    22801 agttttaaga gaggagaatg caaactcact agaattgaaa ggaaaaataa aatttaaaga
    22861 tttttatgaa tcaaaatggc ttcctagata tgaactaggt caaactacta ggtcaaacag
    22921 ggctccaagt aatgtaacaa ttagtaatac taaggatata ttccgattgc acatccttcc
    22981 aatgtttggt gaatatgcaa tgaattatct gaattcaaat actgagataa tatcagatga
    23041 attaacaaag aaatcgaaag aatacgcaaa tataaaaatt atcaaaggct atgttcgctc
    23101 tatgtttgat attgccgaaa tactatgtta tatcgaattc aaccgaacta ccaaaattat
    23161 tcaatgtatt accgcgccta agaaaatggc tttagaagaa aaaaggataa gggaagggaa
    23221 tcaagcatta tcatcagaag agctgactga ttggatagaa gcagtaaaca atgacttaaa
    23281 taatcacttg cttacacttc acgattacac acttttcatg cttaccttat atctaggtga
    23341 tagaaaaagt gagacttatg ctctgcaatg gaaatatatt gattttaata aaaatacggt
    23401 tcgtctcaaa tatgctttag ataaatatca aagaaagaca catacaaaag gttggaaaga
    23461 caccgttatt caggcaccag aagttgtgat gacattatta agagaatgga aatcagctca
    23521 ggctgaacaa ttgttgaaac taaaaattat acaaacacct gaccagtacc tctttaccta
    23581 ttccaaacct tctggagaag taaattgtcc tgttcacgct gactacttaa actatcgaat
    23641 caatgcaata aaaaaaaggc atcctgaatt ggctcaatta tctccacaca aacttcgtca
    23701 tacctatgca actatagcta gggaaggtgg agcaaatatg aatcaaatct ctaatgcttt
    23761 aacgcactcg gatatttcta ctacaaaaat atacgtgaac acacctgacg tagttgataa
    23821 atctgttttt gaagcatttc aaaaaggatt gaataagtgt gattaacatg tgttctcaaa
    23881 tgtgttctta ttataaaaaa agacactttc caaataattg aaaagtgtcc tgaaatattg
    23941 atataaaaag aatattaacg ttttgagaat tgtgaagctt tacgagcttt tttaagacct
    24001 ggttttttac gttcgaccat acgtgcgtca cgagtaagta agccagcacg tttaagagct
    24061 aaacggaagt caggatcaac ttctagcaat gcacgagcga taccatgacg gatagctcct
    24121 gattgtccag cgtatcctcc gccgtttacg ttaacaaata cgtcatatgc gccttttgtt
    24181 tcagtaacag cgaaaggttg attgattact tcacgcaagt cagcatgtgg aatgtattct
    24241 tcaacatctt ttttatttac agtaattttg ccagttcctg gtactagacg tacgcgagca
    24301 actgcgtttt tacgacggcc tgtgccgata tattgaactt gtgccaatga aattccctcc
    24361 tattagatta agtttgtgat gtctaaaact tctggttgtt gtgctgcatg tggatgttct
    24421 gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc
    24481 atacctttta cagaagtttc gatcaaacga cgagaatttt tagcacgcaa ttcgccagca
    24541 gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt aataaatttt gtcagtcgct
    24601 tttttacctg ttaatttcac tttgtctgca ttgatgacga tgacgaaatc gccagtatcc
    24661 aaatgaggtg tgaaagttgg tttattttta ccacgtagta cagatgcaac aactgttgaa
    24721 agacgtccca atggaacatc agttgcgtct actacatacc atttacgttc tacttcgcct
    24781 ggtttggcca tatatgttgt acgcacgtta ttttcctcca atttaagttc tgatgtttga
    24841 catacacttc gagtttccgg ggctctttgt ggggcaaaca ataccattta acatattacc
    24901 cgctaatcta gtcaatgtca acgtttttct gcttgattga aaaatttata tgaaacttta
    24961 cttcttttct ttcttttata taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag
    25021 gctgtgacaa aagtcttgtc acaacccctt attttgaata aacggtgttc tcctgccaac
    25081 tcctcggtca caagtcacaa tatccataaa acataagaag ctgtttttta aaattgtttc
    25141 ttgtgactcg gagctgaacg gcaggcttac aacctcttgg taaacggtgt tcaatcagct
    25201 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttga cgtttgtttc
    25261 tctaatactc aggtcaaaac gctgatttca caacctcttt ttcggtcaag ttcaacaaca
    25321 gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt
    25381 tttctgtggc tgttttgaat tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc
    25441 cagaaacttc gattcctttt tggtaaagat ctgctagata acgatagcct gcaacaagtg
    25501 tttccaaacg ttctggaata gtacgatccc agcggccaat ttcatcaggg atttttgtgt
    25561 tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg
    25621 atacttcatc taactgagcg ttcaaatcat ccatgaactc atccatcatt gggtgcaatg
    25681 taaggaaacc tggtcctctc atgtaccaat gtgtttggtg aacaacaaca gacatttggc
    25741 ttagatctgc aactaattga tttaatactt ctttggtttg ttggaatttc atctttcttg
    25801 cctcctaaca ttttcttaca cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa
    25861 atcatttacc tgagaacgat tttcagttcg cttttttatc cgtaaagcca atcatttttc
    25921 agaatcagct aattcttctc gcagttgagc aaattgctga taagtctgtg ctaaatgctt
    25981 ttgtgtcgaa acaaataatg gatagatttt ctgatatttt ttatgatcag tttcttgtac
    26041 tccaacttgc tctttcaatt ctatcaattc ctgtgcttca ctgatacttt ggatctttcc
    26101 gatgcttctc cagcctaaaa tcacagctcc taatgcggat gcttctgtga cagtagggaa
    26161 aacaagcggg taacccagta catcagccat catctgacga aaaatcggag attgagcaaa
    26221 cccgcctgta gcacggatct ctgtgagctg attttcctgg caaactcctt ttaaaatccg
    26281 ataaaggttg agacagatcc cttctagaat cgccttcatc atttcttttt ctgtatgttg
    26341 ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc
    26401 aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct ggcacaaaag cgatagccga
    26461 aagcagtgca tcatatggat tttctccttt ttctttagca gattgaacga cttcctgtaa
    26521 aaatgtatgc cctgcccaat caaagatgcc agcaccattg ctagtcgctc ctcccactac
    26581 ccaatgtttc tgatccagaa cataacaaaa cgtctccatt tctggatgga actgaggctg
    26641 agtagtaata aaacgaagtg ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc
    26701 aaaagcacct aaccctagat tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat
    26761 atcatttggc aaacctagtt ctgctgctgt ttttgatggt agacctgtta cttgataggt
    26821 ggaaggaaag acttgcggca attgctctat tcgaatgttc aagtagtcaa gcacttcttc
    26881 tgcccattgg aattcatgga tatcaaaata tccagttcct gaagctgttc cgtagtcgct
    26941 gatcagttcg ccagacaatc tataaaagat atagtctttg ataccgacaa aataagcagt
    27001 ttttttgaat aattcaggtt ggctgttttg cagccacttg attttgttca atggtgtcat
    27061 tggatgaatc ggcgtcccag tctgccgata aaattctttg ccaagcacgg tatatttcag
    27121 tttttctgct tcgtccgctg ctcggttatc tgcccaagtg atcattcgag ataacggtgt
    27181 tttctcttca tccaacaata tcaggccatg catcgcagag gagaaggaga caaattgaat
    27241 gattgtgtct gaaggcaact gtttaattac ttcttttata acggtgcaga caccttgaaa
    27301 gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa
    27361 ataagcggat gctgcttctt gggcttcatc tgtgaaagca actgctttgg tttgtgtggt
    27421 tccgatatct aatccgattg aaacgttcat tttttcacct caaattttat gtatgtacaa
    27481 aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg ggagatggcg tgttacttta
    27541 ttttatcatt ggttgctgta tcggatcatt tttatgttta accgcccagc ggattccgtt
    27601 aggtcactct attatttatc ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg
    27661 gtatgaattg attccgattc tctcgatcat cgtccaacga tttcgctgcc gctattgcag
    27721 gtgtcggttg cccttctact atttgttggc agaagcctta tgtggtggtc tttttgcctg
    27781 gttctttact ttttcttccg caagaaattt ttcgacgttc atatggatgc tttcggcttt
    27841 gctattttcg ttgatggatt tattttactt catggtccat tctcacacgc tattctgttc
    27901 ttgggcaatt ttatggattt tttggttgca ggccggcgtc ttccaatggc aaagtgtgct
    27961 gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt caatcctatc tcggagctgg
    28021 cgatactctg ttgatgcttt cttggagtgg tggcttgtct ctagaagagt tgcttcagat
    28081 cttgtttctt gctagtctgt ttggaatatc cgttttttct gtttacttta tctgtcataa
    28141 aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc agtgcgttgc tgattgtcct
    28201 tcatcttagg tattgaaaaa aggttgtgac aaacgacttg tcacaacctc tgatatatag
    28261 tgttctctta ccaaatcctt gtgacttgga gctaggcgac aggttcacaa cctctccact
    28321 aaatggtgtt caatcagctg accctcagta ttagctcaaa atgctggttt tacaacctct
    28381 gatatacggt gttccataag tagctttttc ggcttcttat ttcttgacgc cccaatgtcg
    28441 acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga ttgtaatgga gcttgttttt
    28501 cttgtaataa tcctgatggt aatcttctgc aggataaaat tcgcttcttg gttcgatttt
    28561 tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat gcttgttttg aacgctctgc
    28621 aatatttcgt tcttcttcat cattatagta gatcactgga cgataagaat caccgcgatc
    28681 agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc caatagattt ccaccaactg
    28741 atcataagaa ataacttctg gatcatattc gatctctact gcttctgtat gccctgttgt
    28801 gcctgttgtt acttgttcat aagttggatt tggtacatgc ccgccagtat aaccggatgt
    28861 cacagaaatg atccctggct gctcttcaaa tggtttgacc atacaccaaa agcatccgcc
    28921 tgcgaaaact gctttttttg tcatataaaa tccccctttt ttctcatcat accttgattt
    28981 ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg gaaactatta tatacgtgat
    29041 acggcttctg ataaatgcca aaacgaacgt acttgatcaa aaacggttaa atcaggaatc
    29101 acatattcag cagaaggaag atttatgttt tcacctattc ccaatacatg catgttacca
    29161 gccaaagctg cttgacaacc agcttctgaa tcttcaatga ccaagcatgc atttggcggt
    29221 acattcaatt gctgtgctcc tttgagaaaa acttctggat caggttttgc ttttgatact
    29281 tgcgtgccgt caataaggac atcaaaataa cttgtcaaat tcgttttttc taaaatgagg
    29341 cgtgcatttt tgcttgctga tcccaatccg atcttgatgt ttgttttttt caagtactca
    29401 agaacagcta ttgcaccagg caaaacggat gtttcatcca ttttttgaat cgcttgaaga
    29461 tatagattgt ttttttgctc agcaaaggct gatttctctt tttctgagaa tttctgttcc
    29521 atttttccaa atgacagcaa tcgctctagt gactcggttc tggatattcc tttcagttgc
    29581 tcgttttctt tctcagtgaa tggaatggac agctggttag ctaaaaccag ccacgcttcg
    29641 taatgataac gagctgtgtc tacaagaacg ccatctaaat cgaatagtgc tgctttgatt
    29701 ttcatcgatt acctccttga aaatgaacga cttgaagtaa agatgcgatg tttttagctg
    29761 tgcgatgaag attatcagtg gcttgctgta aagcttcttg taaaggcaca gctgcaggaa
    29821 ggatcggaaa aacggccgaa atgccaacat ctgctgcttt ttcgcttcct ttaccaacac
    29881 ttccgcaaat agctatcaca ggaacgccct ctggtactct tctagcaatc ccaactggcg
    29941 cttttccttt tagactttgt ccatctagac gtccttcgcc tacgattacg aggtctgctt
    30001 gctgtacccg tttatcgaac tccagtaaat ccaatacgta gtcgatgcca gagactattt
    30061 tccctgaagc aaatgctaac agtcctgcac ccattccacc accagcacta ctgcccgatg
    30121 cctcgattac ttgaggggca aatctcatat aaaaatcatg catggcttgg tcagccactg
    30181 ctaattcatt ttctttcaag cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc
    30241 cgcataaaag attagtaaca tcagtaatga cagtgatttt ggctttaatc agctcaatag
    30301 gcacgttcgt tttatcaact gtagcaattt tacctagatt ttctcccagt gcttctactt
    30361 tttgattatc ttgatcgtaa aaagcataac ctagaccttc tgccatgccg atgccgccat
    30421 cgttggtact acttccgcca acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt
    30481 gtcgtatcag ttctcctact cctcgcgtgg ataacctaag tggctgttgt tggtttagtg
    30541 ggacatcttt caatccgacg atttctgcca tttcaaaaat cgcagtttga ccgtctgaag
    30601 cgtactggat ttctttgttt ttgccaaatg cattagtaac gactgctgtt tgccgcttca
    30661 accttaaact gtctgctaat gatgaaaccg ttccttctcc tccatcgcct ataggtagca
    30721 gatcatattc agcctgaaca aaaacttctt gaaaacctga ttgaatggct tgtgctgcct
    30781 ctaaggctgt cagactttct ttgaaagaat ctggcgcaat cagaatacgc attattttcc
    30841 cctccgttcc aagatcagtt ctttttccag ctggtattgg tctccattga caaaaatagg
    30901 taatgtttct ttcgtctctt tttttattac tatttgtttt tttgtcaaaa caaatagcaa
    30961 cctgtgattt ttccaaataa aaggaaaaga aattttttcc catgctgaag gaagtcttgg
    31021 agaaatagaa agagttccat tcgttactgt aatacctgaa aaaccaaata taactgctag
    31081 ccatagtgct cctaatgaag cagcatgaat tccgtcatct gatgaatgcg gatctggtcc
    31141 taaatcaatc aggcacgctt cttggaataa atgatacgcc cattccattt cttggatacg
    31201 gcaagcctct atggcgtgaa tcgctttact caacgatgag tcgtggattg ttttgttttc
    31261 atagtaatgc aaattcttag aaacgattgg ttgagaaaaa tactcaggta ataagtataa
    31321 cagcatgaca agatctgctt gcttgctgac ttggagttca tttacttcgc tacgggcata
    31381 atcaagtaaa atagactggc tgccaggacg ttctttgtaa gtggtcaaat caataactgg
    31441 cttactgaga tacgtgtcat cttgcggaat cagctgttct ttgttaggca ctggcaaata
    31501 taacttatct aaaaaatttt gacattcttt caaaaaaagc ggatcgccag cttgatattt
    31561 aacagctaag gccacattat aatgcgccag atagttcgta tatgcattat tgtcgatata
    31621 ttctgtgtat tcatctggac caatgactct gtgtatctct aaatgatcat cgacttttac
    31681 agctctgctc agccaaaaag ctgctgtttc atgaatgagc gtttttccct ttgtttctaa
    31741 aaacgattga tcctctgttg cttggacata ctcttgaacg gcataagcaa ggtctgcact
    31801 gatatgatgt tctgcggcag ctgaagcaac ttcttgtctt gttccggttt tgatattcaa
    31861 tgcagcaaat ttgggtgtct cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc
    31921 accttgatag tgataatctc ttgctttttg ccgtgcgcct tctaatcgat cataacggta
    31981 cagcagcaaa ttctttgcta cttcaggaaa ctgatataaa aagaaaggca ggataaaaat
    32041 ttccgtatcc cagaaaacat gccctttata accttcgcca gttagtccct tcgcccctac
    32101 actaacactt ggatcattgc ggggagtcat aatctgtaaa tggtaagcag cgaagtccat
    32161 cgctaactga tcgaaagaag aatcgccttg gatcgttatc cgatgctgat gccaaaaatc
    32221 tgtccaataa gacatcgact gctctaaaat ttctgtataa ctaacgcttt tctgggcttg
    32281 ggcaatactt ttcttaaacg ggtctgtttg ctgaagagag gtagagagca cgatgatctt
    32341 ctccagcgta aaggacgtat tttttctgat cttttctgaa aaggaagcgg tgatccggcg
    32401 attttttgct tggaatacac ctgctttatt gaatttagct gcaatcccaa tatcaatttt
    32461 actttctgtt gtctggtagg atgcactgat caaatcgtca ccaaatactc gcagttcttt
    32521 ttcaaccaac tgctgtgtac caaaattggt ttgctgtcca tcgatacccg ttttcacatc
    32581 tacttggcag tcttcggtca acggcttgat gaccactttc agggctgcct gatggagatt
    32641 attttgatca gcaacacgac aagtaatgac ttccactttt tttccttgag cagtttcaat
    32701 gagcgcatag cgtttaagct ctcccatttc taaatctagt gcccgctcgt atgcaagaac
    32761 tttcatcgtc tcaagagaaa aatgttcacc gtcgattttt atttcccatc tgatgacatc
    32821 tgggagattc actaactcgg ctgattctgc tcctaaaggt cggttatata caccagctgc
    32881 aaacattccc cttacttgat tccgatagtc ttcctcctgg cttcctctga taccaaaaaa
    32941 tccatttcca atggtcatct gactcgcata cttattgata tactctaaat caaactgttt
    33001 ttcttctact ttcataaggt aataacttct cctgtttctg ccgaacgata taaattttcc
    33061 accagattct ggatgacata tccttgccag ccatctgcaa tcatctgagt ctggtcaccg
    33121 agcgcccgat tcacaaaatg agccatactt agttcatgtg ggtcattaac tggcgtggtc
    33181 ttttgtaagg aaaccagctt tccatctaca tcttcgaata gttcgcctgg atacagatta
    33241 gcacctgcct gatcgccaca aaaagccaca ttcagtttgt cttctctgat attcaaagca
    33301 aacgaagtat tcagttggat gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca
    33361 tcttctactg tgaatttgct tggatcccat ttcccgaatt tccctgaatt ttttttcgta
    33421 ccaatttttt ggaagctgta cgcccgaacg ctttttactt taggaaatcc agtaatatac
    33481 atcgctgtat caagcatgtg aatgccataa tcgatcaaag gcccgcctcc ttgaagttct
    33541 ttgtcgataa acgttcccca gcctggaaca ccagaccgac gaagcgcatt cgcttctacg
    33601 taataaatat ccccgagaac atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa
    33661 cgatgctgga atccataagc caaaatctgc ccgtttttat cggctgtctc tgccatcttt
    33721 cttgcttgat cagctgtcat agctggtggt ttctcgcaca tcactgaagc attagcttcg
    33781 agggcagcca atacatgttc gaagtgaaag cgattgggtg tacagacact cacgatatcg
    33841 ggagaaatct cattcagcat ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt
    33901 cggcaaaagt cttctgctcg ctggctgact ggatcaacca cagcggccaa ttctaagaca
    33961 tctgatcttt tttgataata agcagcatgt actttttctg ctacttgtcc agcgcctatg
    34021 attgctactt tcttcattta tttctccctc ctttgcttat ttacttattg ttattctttc
    34081 tctattcttt ctctaaatca tttaaatgac gcgctgtttc cagacagttt ttcaagtatt
    34141 gcaatgattc ctgataaatt ttcacttcat tttctcctcg caaacgacat tcatatacca
    34201 tataaccttc atactcattt tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc
    34261 cagatcctgg ttggtagcga tgattatctg ctaaatggat atgacccaca tactcttttt
    34321 gcgtagtcaa tgcttttgaa atatcatctt cttcaatatt catatgataa aaatcagcga
    34381 tgatctgcac atgtttcaat tgattagagt caatgtaatg acgcgcatcc gctaacaaat
    34441 tgatcatatg gtcttggtat cggttcaaag gctccaaata aataaccgta cctgtttctt
    34501 cggctacttt atctaaataa cgaagtgatt cgctgactgc tttatagtct ccttctttgc
    34561 ttcggggtga aaccatcggc ggtaatcgat aagtgaacat tccccaagct gctgggataa
    34621 cgattccttt gcctccaacc tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac
    34681 cattaagacg ccgttcttcg ataaagtcac cgatccaccc atcgtagccg ttacaagctg
    34741 tcactactgg gatccctgtt tctttgattg cctgtttgac tgtgtcgata ttttctatta
    34801 acaacttacc gtcgatttcg tattcttcaa atccacagtc tttgataaat ctaaattttt
    34861 ctaaaatgtc tcttgggaaa aatgcttgat cttgcgtacc gaatttcatt gttctcctcc
    34921 tcggacttga acgcccattt tgatactttc ttctgggtgc tggtctacat aacgcatgta
    34981 agcttctgcg ctttctgcaa atggcacaac tggatcaatg atttcttcac aatccaagta
    35041 accgttcatc aataattccc aacatgtgtc ctcgattcgt ttacggttcc atcttgggta
    35101 atctgggttt ggttcactag atgctcgagc aaagacgatt tttgcattat tgaaatgtgc
    35161 ttcgcgacca agattaaacc cagctggaaa aggtttagca aaggcaacat aagaaatgat
    35221 cccgccatag ccgatccctc ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc
    35281 aataactgaa tctactccac gtttatctgt caattccttt agacgaaaac cgacatctgc
    35341 tttcatagga tctagcgtgt aatcggcacc gtatttttct gctaattctc tacgctgcgc
    35401 tatgggatcg atcccaacga tggtagtagc acctgctttt ttagctaatt gaatcgcaat
    35461 ttgcccgata gcgcctaaac cgaagattgc tacagcatca cctgctcgaa catgtgcttc
    35521 ccggatacca cctaaagcga attgagcagg atcgtaacat actgcatttt tccattgatc
    35581 caaccgatca agtttacgta gtttataatt atcaacaccg tttgtaatca gcgtttcacg
    35641 aatgggtcca taagtgcaga ctaactctcc tttttgatac tctgtcacca aactgcccgt
    35701 ttcttcgatc gtaccgacaa tcatatttcc taattgaaat tcgccaaata ccacaccgcg
    35761 ttggcttcct tcttctcttg gcaagaatag gcgccattct tctgaaaatt cttcatcgat
    35821 aaatggactt aatcctctaa agtcgacaat ttctgttcct ggttttggag tagcgaaaat
    35881 tactcggata cgtacttcgt tcttttcgat ctcacggtct tcgtaatctt tgaatccggc
    35941 tattcttggt tctaatgcaa ctaatttttt catcttttct ttcctctttc ttttcttttt
    36001 atccttttac gccgccttcg actaaatcac ctttaataaa tcgttcagat aacgcataca
    36061 taatgatgac tggtaaagcg gtcaccaatg aagcggccat cattcgtccc caaatgtagt
    36121 ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca
    36181 ggaaaatcga agcaaataaa taatcattcc acgaaaccat aaagcaataa acgaagacag
    36241 aaatgattcc agaaatagcc aatggtataa tgatccgaat aatgatctgg atccggctaa
    36301 gaccatcgat cattgctgct tcttcaatat cagtcggaat cgtgtcaaaa taacttttta
    36361 gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt gataatcagt gctgtattgg
    36421 tatcatacaa acctagaccg gaaatgattt taaataatgg aacaacgatc aaaataccgg
    36481 aaaacatata aacggtgtaa aagctagcat tgattttcga gcgagctttg aagcgtagtt
    36541 ttgacaaagc gtatgccccg ctgatcccaa tcactactgc agttacggag gctatcaagg
    36601 agatcactaa actatttttg aaatacatga tgaatggaaa aatctcagca ttgaaaatat
    36661 cctgataatg ttcaaaagtc cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt
    36721 cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta
    36781 atagcaacaa aatcgtcgcg taaaataaga ttttcctgct ctttttattt ttcatttcaa
    36841 gtccaccttc tttcgtgaga gcaagacgac cgctgcaata atgatgaaca atagaatcga
    36901 tatagctgct gcttttccaa gatcattgaa cgcaaaggct gtctgataca aataaacgcc
    36961 taagatattg actttattcg tcagtagata gacatctgta aacatataga acatccagat
    37021 agatcgtaaa acaatcacag ttgctagtac tggtttgatt gccggcagtg tcacgatctt
    37081 gaacttctgc caagcatttg caccgtccat ttcagctgct tcataaagcg ttttgtcgac
    37141 tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga tagtatctcc agatagcaaa
    37201 caaaacgacc ataaagaagc tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa
    37261 aagctgcaac ctatctacta acagatcatt gacgattcca taactattat tgaacatata
    37321 tttccaagca aaaatcaaag aaatagatgg cgtaacataa cttaataaaa tcagtgaacg
    37381 caccgttttc ctaaatctga acttgcggtt aagcaaaata gctacggcta atcctaaagc
    37441 tgtactgcct gctacaacta agaatgtgta agcaactgtt aaacctaaag aacgataaaa
    37501 ttcactatca cttaaaattt caagataatt gcttaatccg ataaaaacgg cgtctgagtt
    37561 aggattcagc ggcatatcga aaaaactgat ctgaatattg gaaagcattg gaatcagcac
    37621 taaaacagct aataaaagca tgcttggtgc taaaagtccc atggctaatt ttaagtcaga
    37681 atgtttcctt ggacgattca ttgaaacctc cccctgataa tctgccaata gctatctact
    37741 tttttcgaac accattggca gaacttcttg ttatttcgta tttttttgtg cgtcagctaa
    37801 agcttgatcg acatcttcgt ttccgacagt tacttggttc actgcttttc cgatcacacc
    37861 tgagcttgtg atatctccca ttttcgtgaa attcttttcc ccgactagtc caaagacttg
    37921 tacttcatca aaagcactag caatctcaga tggcagctcg ccaaaagact tgatgacttc
    37981 attttctttg taaccatcca gttcgactac ttgtttattc actggttgag caccaccagg
    38041 agacatcaag acccactttt ccatgttttt cggttgagat aagaaagctg tgaatgcttc
    38101 agcagcctct ttttgtttct cgtctagacc agaagaaatc gtcaatccag atactgtgcc
    38161 atagacagct tgatcttttt gtgttgggat agcaaaaccg atattttctg agttccccgc
    38221 ttcataaaca gaaggcaaaa tgtaagttga atagattgcc attggtgctg ttccattcat
    38281 gaaagcatct ttgatttctg tcacatcatt agagcctggc atcgtgtatt gggataagtt
    38341 ttgataaaac gacaatgctt ctctcatctt ttccgtatcg atcgttacct cgccttcatc
    38401 atccaaaaca tttgctccat ttgacaaagc aaattgagag aacgcttgct cactcattgt
    38461 tccttcagca gtaggcatag caattccata ttgtttattc tctttatctg tgaaatgttg
    38521 agcaattttt aaaatatcat cccaattttc tggttctgaa aaaccagctt ctgataattt
    38581 ttctttgtca taccaaatac cttgaaccca gccgctaatt ggtgcagcaa tatagctttt
    38641 tccatcttct gaacgaacaa ggtttttggc tccgtcataa taattatctt ctccagcttc
    38701 ctcgataacg ttttggactg ccgtctgatc aatcagttca tctttatcca tcactttagc
    38761 aaaatcctga ctgacttcca ttacttctgg taatttaccg gaacgagcta acgtaacgat
    38821 ttttgtattg aacgctgatt cttctaccgg cacttgcttg attttgatgt ctggattttc
    38881 tttttcaaaa tcagcaacca gttcattgat cacacttaat cgttcctgtt cgacagaaga
    38941 gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag ctttgacttt caccacaagc
    39001 agtaagaaga aatccagtag ctagcagaca ggtagtaaca ataatttttt tcattttttt
    39061 ccacctctta ttttattatt ttcagccaca tatattcata cggctggaat atcaactttc
    39121 gttcttgact gcaatggata atttgtccag aaaacaggtc taggtaatct ccttttggta
    39181 aaagataacc tgctgattga ttggaaaaat tatggataac taagactttc tcttccttcg
    39241 cttcgcgaat aaaactgata aaagaatctg ctgagtccag taagtaaatc aaactattcg
    39301 gatggaaggc tttttctttt tttcgaatag atagcatctg ggtcaattca ccatatactg
    39361 ctgttcttaa tggatctgtc tgcaactctt tctcaatatc tgctaacaaa aatttttgcc
    39421 gattgatttt tcgattctct cctgtttttt ctacgccttt gtaatcattt cgacttccca
    39481 gcacactttg aatataaatt gccggcacgc ctggtaacgt taccaaaaat gaatgagcag
    39541 ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg agaacttaac gcatctagat
    39601 acgtagcgtt tatctcatat ggactggtcg ttccatctgg gtttttcttg tagtttactc
    39661 gggcaccctc tttcttcagc tgttccacta attcaaggat cgttttttcc ggcagaatac
    39721 ctcgtaccgg attcaatccg ataccgtcat gggaggctaa aaaattaaaa aaagttgttt
    39781 ttttcgatgc gaaatcgatg gtttttaacc attctttcaa cttacttgta ttacccgaac
    39841 gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata gaccatctgt gcttcattct
    39901 cgccattccc aaaataactg atattatctt tgtgtggcac atttgtctca gtgatcaata
    39961 ccgttcctgg agctgcttca tcagcgattg cacggaataa ttggatgatt ttatgcgttt
    40021 ctggcaggtg tatacaagaa gttcctagtt ctttccataa aaatcccaca gcgtccaagc
    40081 gaataaatga agcgcctttt tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa
    40141 cttctggatt tttaaaattc aagtctactt gatcttcgct aaaagtcgtc catacgtgtt
    40201 tttctttccc gtttttgtca atgaacgttg taagtaacgg gctggtcctt ggtctagtca
    40261 cgctgcttgt atctgtatcc agtggcaaat caataaaaaa atctttgtac gtatcattca
    40321 agtttaaata ttcctgaaac caagtacttt cggcagacat atgattgcag acaaagtcga
    40381 acattaaccg tgtttcgctt ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt
    40441 tgattgcctg ataatttaca acggagaaac catcatcaga tgtatatggg aaaaatggca
    40501 agaaatgtac aactctaaat gtatctgcca aatacttttg ataaaatttc ttaaatgtac
    40561 gcaaagttgg ttgctgctct tcaaaaaatt gatccccata agtaatcaag aaaatatctt
    40621 cttcgtccca atgcagtttg cctgcctggc ctttcttctg ctgataaaca ctgacttttt
    40681 gttcaatcat agaggcggtt ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa
    40741 gttttgtata catcggattc ccctttcttt tttgggagcg ttcccaacga tttgatcata
    40801 ttatggaaaa cgcttactgt caatagcatt tttttaaata attttgatat acacaaaaat
    40861 acaaataaga aagcttatgt atcaataatt aatagcttct taaaaaaata tggtctctca
    40921 tgatcatgca agctattcct aaattttcaa aatcatagac aaaaaagaat gtctattgta
    40981 ctatggaatt attctaaaac gctcccaaaa aggaggtgca tataaaattg aaacctacta
    41041 tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac tgtctccaga gtattaaata
    41101 accagccaaa tatctctgat aaagcaaaag caagtgtact tcaagcaatc aaagaattga
    41161 attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa tggttttgac gcgattctag
    41221 cattatctca tcgtacttct catacgacct atggcaatcc ttttttttca gatgttattc
    41281 aatccatttc tgccgtttct gaacaaaata acttcgactt gattctacaa acggcaaaaa
    41341 caccagaaga agagctagaa aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa
    41401 ttattatttt aagctctacg atggatgaaa cacttctttt tgaattggat caatacaata
    41461 ttcctattgt ggtaatcgga aaagtcggaa acgactacac gaacatctac tcagtggaca
    41521 cggataattt ccaggatagt tacgatttgg tggaaacact gatcaagaat ggacaccgtc
    41581 atatcggctg tctccatgct ccgacagaag tttatgtatc caatgatcgt ctcaatggtt
    41641 accgtcaatg cttatttgat caccacctag atatccgtaa tgaatggatt atagacggcg
    41701 gctttcgctt agaagatagc atggcagcaa tgaatgattt aatgaagcta cgagaaagac
    41761 caactgcgat tttttctacc gatgcattaa aatcattgag tatttacaaa gtggcagaac
    41821 aatctggaaa gcagatacct gatgatcttt cgatcgcagg attcagtgat cccttattgt
    41881 cctccctatt ttcgccacag ttgactcgga tagacattcc gacaaatcag ttaggaatca
    41941 aagccacaga acttttattc caactaatcc accaccaata ccctgaagaa aaaagaatca
    42001 ttctttcaac cgagattact ctcacagatt cgatccaaca acaagaaatg tagaacagaa
    42061 ttataaaaga acagaaacac tgaggataag tcagttactc tgttcttttt tcaatcactc
    42121 atccctcgtt accccctccc ggtattttag cgaatttttc ttattttcca tctgttttag
    42181 atagctgtgg ataactataa tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac
    42241 tatttttaac cacgttgtgt ataagttttt atagttcgtt tttttaaaca catttaccct
    42301 tatttcaatc catgttattt ggggatactt atctctaaaa cataaataga ataacagaca
    42361 tgtctactgg ttttgtttta ttcgaggaat tattcaataa tagctagcaa aatattaaaa
    42421 ataaccgctc tatcttccca tcaatttatt ttgcattcaa aaaatgatct atctttactt
    42481 ttgttgattg acttctcttg gaagatgatc gttccgatac cttaattgga tacgaaccga
    42541 tgttgttaag atcctttccc acttttcttc tagttagaca aattatttta gacaaaccaa
    42601 aaaagaggtt gtgacaataa tttgtcacaa cctctttttt aaaatatcta ttttgtttga
    42661 ttgttcattg cgtcttctgc agccatttgc gcttcaatat ctgaaatact gtagacattt
    42721 tcgcttgcta caccgacttc ttttggttcc atgtcacgat aacgttgcat accagtacca
    42781 gctgggatga tcttacctat aataacattt tctttcaaac caagaagagg atctttctta
    42841 ccgcggatcg cagcatccgt caagacacga gttgtttctt ggaatgatgc agcagataag
    42901 aaactatttg tttctaatga tgcttttgtg atacctagta agacaggtcg agaagttgca
    42961 ggaacgcctc ctgagactag cgtattgtag ttttgatctt taaagtctgc gatgtctaat
    43021 aatgtacctg gtaagatatc tgtatcacct ggatccatta cacgaacttt acgcagcatt
    43081 tgacgaacca ttacttcgat atgcttgtcg ccgatttcta ccccttgcat acggtaaaca
    43141 cgttgtactt cacgaagcaa gtagttttca acagataata catcgcgaac ttgcaacaat
    43201 tgttttggat cgattgatcc ttctgtcaat ggtgcaccac ggtggatcat atcgccttct
    43261 gctactttca tacgtgctgt ataaggaaca gtatacgtac gtgtatctgt ttttcctttg
    43321 attgttactt ctttggtacg tgttgcagga tcttctgaga tatcgatgac atcccctgtt
    43381 acttctgtga tcactgcttg ccctttcgga ttacgtgctt caaagatttc ttggacacga
    43441 ggaagacctt gagtgatatc gtctccggca accccacctg tatggaatgt acgcatcgtc
    43501 aactgagtac caggttcacc gattgattga gcggcgatcg taccaactgc ttctccgact
    43561 tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat gtttgcagac accatgtttt
    43621 gtattacatg tgaagacaga acggattgtc actgtttcga cacctgcatc aacgatttgt
    43681 tttgcgatgt cttcggaaat caattggttt cctgcaatga tcaattcatt tgtttcagga
    43741 tgacgtactt ctttacgggt gtaacgtccc actaaacgat cttctaatga ttcgatgatt
    43801 tcatttccct cgcggatggc ttggatttca agacctcggt cagtaccgca atcttcttca
    43861 cggatgatta catcttgggc cacgtcaacc agacgacgag tcaagtaacc tgaatcggca
    43921 gtcttcaagg ctgtatcggt catcccttta cgagccccgt gagttgagat aaacatttcc
    43981 aagacggata gtccttcacg gaagttcgaa atgattggca attccatgat tcgaccatta
    44041 ggagcggcca tcaaaccacg cattccggca agctgtgtaa agttggaaat gttaccacga
    44101 gctccagaat cagacatcat gaagattgga tttttcgctt ccaaaccttc catcagtttt
    44161 tgttggatgc tgtcttttgc agcattccaa acagcaatga cacgttcata acgttcgtca
    44221 tctgtgatca gaccacgacg gaattgtttt gtaatagttt caacttgttt atgggcattt
    44281 tcaatgattt cttgtttttc attcagtaca ctgatgtcag cgatccctac tgtgataccg
    44341 gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt caagcatctt agaagtttct
    44401 tttactttga atcgtttgaa gacttctgcg atgatgtttc ctaagttttt cttcttgaac
    44461 ggtaatacta attcttgctc tttgatatgt gcaggaatat cagtaccagc ttcaacaaag
    44521 tatttgtccg gtgtttggac ggtcaagttg aagtctgttg gttcattcag ataagggaat
    44581 tcaggaggca tgatctcgtt gaagatgatc ttaccaactg ttgtgatcat cgtacgttct
    44641 ttttgccatt cagtaaatgg tttttcacct aatgagttag ggttcacacc gatacgtgaa
    44701 tgtaaatgaa catatccgtt gcgccatgcg ataacggctt cgttttgatc tctgaagacc
    44761 attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt agtagttacc tagaaccata
    44821 tcttgagaag gagtaacaac tggtttacca tctttagggt tcaagatgtt ttgagcagct
    44881 aacatcaaca tacgtgcttc tgcttgtgct tcttcattca atggaacgtg gacagccatt
    44941 tggtctccat cgaaatcggc attataagct tcacatacaa gtggatgcaa acggattgcg
    45001 cgtccttgta ctaaaactgg ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc
    45061 cggttcagca atactgggtg ttccttgatt acgtcttcta aaacatccca tacttcgtct
    45121 tcttgacgtt cgattttacg tttcgcattt ttgatgtttg aagctagttc gcgttgaaca
    45181 agctcacgca tcacgaatgg tttgaataat tcaatggcca tttcttttgg aagtccacat
    45241 tggtacatct tcaagaaagg tccaacaacg ataacggaac gacctgagta gtcgacacgt
    45301 ttacctagca agttttgacg gaaacgacct tgtttccctt tcaacatatg agaaagagat
    45361 ttcaatgggc ggttacctgg tccagtaact ggacgtccgc gacgaccgtt atcgatcaat
    45421 gcatctactg cttcttgcag catacgtttt tcattttgaa cgatgattcc aggtgcattc
    45481 aagtctaaca aacgtttcaa acggttatta cggttgatta cacgacggta aagatcattc
    45541 aagtcacttg ttgcaaaacg tccaccttct aattgaacca ttgggcgtaa atctggtgga
    45601 ataacaggga taacgtccat aaccatccag cttggttgat ttcctgaagc gcggaatgct
    45661 tctaaaatat ccaaacggcg gattgcacgt gtacgttttt gtccttgtgc agatttcaat
    45721 tcttctttca attcggctgc ttctgcatct agatcaacat tatcaagtaa ttgtttaatc
    45781 gcttctgcac ccatagcagc ttggaattct tgtccatatt gttcacgttt ttcgcgatat
    45841 tcgcgttctg tcaacaattg ttttttctct aaattcgtat ttcctggttc gatgactaca
    45901 tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc
    45961 atacgtgaag gaatcccttt aaagtaccag atatgtgaaa caggagctgc taattcaata
    46021 tgtcccatgc gttcacggcg aactttcgaa cgagtaactt ccactccgca tcgatcacaa
    46081 acgattcctt tataacggat acgtttgtat tttccgcagg cacattccca gtcttttgta
    46141 ggaccaaaaa tccgctcaca gaacaaccct tcgcgttcag gttttaaggt acgatagtta
    46201 atcgtctcag gtttttttac ttcaccgtaa gaccagcttc tgattttttc gggagaagcc
    46261 aaacctattt gcatactttc gaatttattt acatcgatca aaaggggttc cctccatttc
    46321 atttaatggc aaaccgcaag acggctgatc ttcgaccgcc ttgcagctca tagaccggat
    46381 tagtctaaat tatcttctgc ggtttcaaag ttttggatga catcgtcttt ttcatcttcc
    46441 acttgttcag cagctttctt ttctaattct ttggcagttt gctgttcggc aaattttgtc
    46501 aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct
    46561 gtttcttcga tatccaagac acgcatgtcc agacctagtg attgcaattc tttcaccaat
    46621 acgcggaatg attccggtac acctggtttt ggaattggtt cacccttgac gattgcttcg
    46681 tatgttttca cacgtccgac tacgtcatct gatttgtatg tcaagatttc ttgtaaagta
    46741 taagccgcac cgtaagcttc cagtgcccat acttccattt caccaaaacg ttgtccacca
    46801 aattgagctt tacctcccaa tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt
    46861 gcatgcaatt tgtcatcgac catgtgagca agtttgatca tgtacatcac accgacagac
    46921 acacgtccat caaatggttc acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg
    46981 gccataccgg cttcacgaac tgtttcccat acgtcttcat cacttgcacc atcaaatact
    47041 ggcgttgcta cgtggatacc taactgacgt gcagccatac ccaagtgtaa ttccaatact
    47101 tgcccgatgt tcatacgtga aggcacccct aaaggattca gcatgatatc gattggtgtt
    47161 ccatcaggta ggaatggcat atcttcttcc ggcataatac gggatacaac ccctttattt
    47221 ccgtgacgac cggccatttt atccccttca tggattttac gtttttgaac gatataaaca
    47281 cgaactaaca tgtttacgcc tggtgacaat tcatctccag cttcacgagt aaagattttc
    47341 acatcgtgaa cgataccgcc gccgccatgt ggtacacgta atgatgtatc gcggacttca
    47401 cgagcttttt cgccaaagat cgcatgcaat agacgttctt ctgcagataa ttctgttacc
    47461 ccttttggcg tgactttccc tacaagcaaa tcgccatctt ggacttctgc accaatacgg
    47521 atgataccca tttcgtctaa atcttttaat gcgtcttccc cgacattagg gatttcgcgt
    47581 gtgatttctt caggtcctaa ttttgtatcg cgggcttctg attcatattc ttcaatatgg
    47641 atagaagtgt atacgtcatc ttttaccaaa cgacggctca tgatgatcgc atcttcatag
    47701 ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt ggcctagagc catttcgcct
    47761 tgttccatag atggtccgtc tgccagagta tccccttttt cgactttctc acccaaatga
    47821 acgattggac gttggttgta gcttgttccg gagtttgaac gacggaattt tgttacatcg
    47881 tatttatcta atgcaccgtt gtcgcggcgt acgcggattt cagaagcatc aacgtattca
    47941 acgacaccat catgtttaca taacaaagca gctcctgagt catgcgctga tttgtattcc
    48001 ataccagtac caacccatgg tgcttgagga ttgatcaacg gcactgcttg acgctgcatg
    48061 ttggcaccca tcaaggcacg gttggagtca tcgttttcca agaaaggaat acatgctgtc
    48121 gcaacggcaa ctacttgttt aggtgatacg tccatgtagt ctactttatc aatagatact
    48181 tctaagtttt cactttgagc acgagccata acaacgtctt gagcaaatgt tccatcttca
    48241 ttcaatgggc tgtttgcttg ggctacgatg taatggtctt cgatatcagc tgtcaagtag
    48301 tcgatttggt cagtaacacg acctgtttca cggtctacac gacgataagg tgtttcgata
    48361 aatccgaatt tatttacttt cgcataactg gacaaactat tgatcaaccc gatgtttggg
    48421 ccttcaggag tttcgatcgg acacatacgg ccatagtggg agtagtgaac gtcacgaact
    48481 tcataaccgg cgcggtcacg agtcaatccg ccaggtccta aggcgaaaag acgacgtttg
    48541 tgtgtcaatt cgccaagagg gtttgtttga tccatgaact gtgacaactg agaagaacca
    48601 aagaattctt tgatacttgc cacaactgga cggatattga tcaattgttg aggtgtcaat
    48661 gtttcagtat cttggatcga cattctttca cgaaccacac gttccatacg tgctagcccg
    48721 atacggaatt ggttttgtaa aagttcaccg actgaacgga tacgacgatt tcctaagtgg
    48781 tcgatatcat cgacattgcc gataccttcc atcaagttca agaaatagct cattgaagca
    48841 atgatgtcgg ctggacgaac cgtttttaca gctgcttctg gatagccgtt tccgatcacg
    48901 tttacttcac gttcagggtc tcttggtgaa aatactttga tgacttggac agtcattgga
    48961 tctgtgacca caccgtcttc tgatggatag taagtcactg aatttaggcc attatcaatg
    49021 aaaggagcta gtgtttccat gacttggtga gtcagaactg ttcctttttc aacgatgatc
    49081 tcgcctgttt ctggatcaac taatgtttca gctaatgtca gattcaatag acgtgttttc
    49141 aaatctaatt ttttgtttac tttgtaacga ccaacgtttg ctaagtcgta acgttttgga
    49201 tcgaagaaac gagcattcag caagttacgt gagctgtctg ctgtttttgg ttcgcctgga
    49261 cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc ttgagtcgct tgcgttttta
    49321 tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc
    49381 gtatcatcgg aaccaaatcc taacgcacga actaatacgg ttaaaggaat cttacgtgta
    49441 cggtcgatac gtacataaga gatgtctttt gcatcagttt ccatttctag ccaagctcca
    49501 cggttaggga taacagtaga accgaatcct tcttttccgt ttttgtcgac ttttccatgg
    49561 aaatagacac ctggtgaacg gactaactga gaaacgatta ctcgctctgc cccgttgatg
    49621 atgaatgttc cttgttctgt cattaatggg aaatcaccga agaatacttc ttgtgctttg
    49681 atttcgccag tctcgcggtt tgtcaaacgc aatgttacat gtaatggtgc agagtagttt
    49741 gcatcatgcg cacgagcttc tgctactgta tactttggtt ctttcaattc ataatctaca
    49801 aattccaaag atagattgcc attgaagtca tcgattggca aaatgtcttc aaacatttct
    49861 ctaagtccct catctaagaa ccattggtaa gagtctgttt gaatttcgat caaatttggt
    49921 aattccaaca cttcactgat tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt
    49981 actacgtgtc cagccaagct cttcacccct ctaaaatgtc attcaaaaag actacccgat
    50041 caattgttac aataatattt caaatattat ttttaggtaa caacagacat tttttgcggc
    50101 ttttaggcaa aaaaaaacca aaaatagaac attctcttga aaccttactt cgtgagcttc
    50161 tacttttgtt tccttatatt aaagccgaaa cggacaatat ttcgtttctg ccttttcttc
    50221 atcagatagt ttttgcaact tcatatacta cacttatttt ctataaaagt caacacttcc
    50281 tggcgtttct tctgcttttt cgcaacattt tcttaatgtt ttcaatttta tgttttcaat
    50341 tatctgacaa ttcttaattg acagaccttt ttatctcggt tatactttag aaaattcatt
    50401 ttgtggagga acttctatgc gaaccaacat caaccaaatc gcaccaacac aactgaataa
    50461 cttttacttt agctacttta gataggtttg tattcatcat gatggataca aacgttaaac
    50521 gacagtttgt atctatgatg gctaaagaac tttgtattgc agcaaaaagc cacgtagaca
    50581 aaatgctaag tggctgagca gtgaaaatac aaagtaaaag tatcaagcat tctacttttt
    50641 caatttattt tccgactttt caatccattt ggacattttg tctgaatgga ttttttgttt
    50701 cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc
    50761 ctgctactag ctttaggagg ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga
    50821 caaaaaacaa ttggcgtatt gcaacttgta caacataact cgttagatgc tgcctataaa
    50881 ggattcaaag aaggattagc agaagaaggg tataaagaag gcgataatct aacaatcgat
    50941 tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa
    51001 gaagatcctg atttgctact tggaatcgcc actccagcag cacaatcttt attaaatgaa
    51061 acaaccacta tcccgatgac tgtcacagcg gtcacagact tggaacaagc ccatttagtc
    51121 acatccaatg aaaaacctgg cggcaatgtg actggcacga cagatatggt gccaatcgaa
    51181 aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca aaacaatcgg catcatgtat
    51241 aacaacggtg aagcaaactc taagatccaa gctgatttgg cagaaaaaga attgaaaaaa
    51301 gcgggcgtca aagtcaaagt actgacagca aattccacaa atgatgtcca acaagtgacg
    51361 aaaagtctag ctaaagatgt agatggaatc tatattccta ctgataacac gttcgcaagt
    51421 gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca
    51481 gttgatcaag tcaaagacgg cggtctagca acagtaggaa tcgactacga aagcttgggc
    51541 aaacaaacag ggatcatggc agcaaaaatc ttagacggca agaatccaag tgaacttcct
    51601 gtcgaaacag cgaatgattt ggaactgtat gtcaataaag acatggccaa agcattaggc
    51661 atcaatccag acagcatcaa agaagctgaa taaaacacag gaggattctc atgacaaatt
    51721 tgctctctct tttcttatca tctgcatcgc aaggggtctt atgggcccta cttgcgattg
    51781 gtgtatacgt cactttccgt atcttggaca ttgccgactt gactgctgaa ggaagctttc
    51841 ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg ctatcatcca ttgacagctt
    51901 gtcttctagg ctttttaggg agtgcggcag cagggcttgt ttccggactg cttcacacaa
    51961 aattgaaaat tcccgccctt cttgcaggga tcatcaccat gactggttta tattctgtca
    52021 catcccgtgt gatgggtgcg ccgaacatct ctttattggg tcagccaacg atctttcatt
    52081 gggtcgagtc attcggcata acaaaagaca acgctgtact gatcgtcggt ctgctttttg
    52141 ctttgatcgt tgtatgttta cttgttttgt tcttacgtac agaaacagga cttgctattc
    52201 gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg gatcaacaca gataatatga
    52261 agatcatcgg ttatatgatt tctaatggat gcattgctct ttctggctcc ttgcttgcgc
    52321 aaaacaacgg ctttgctgat ttaaattctg gtattggaac gatcgtaatt ggacttgctt
    52381 ctattatcat cgcagaagtt ttgttacgta accagccact atttctccgt ctcttgactg
    52441 tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg
    52501 aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt gatctgtctt tctttcccgc
    52561 agatccagca aaaattccat ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga
    52621 cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa ggaacgatca acgaaaatca
    52681 tgtcctgaaa ggcattaatc tttccttaga tcccggcgac ttcgtgacga tcatcggtgg
    52741 aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca
    52801 gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa
    52861 atccatcagt cgcgtattcc aagatcctaa actagggaca gccgttcgtt taacggtaga
    52921 agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa
    52981 accacaagac cgaacatttt tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa
    53041 tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt
    53101 gatggccaca ttacagcgtc cagaactgat cttattagat gaacacactg cagcgcttga
    53161 ccctgctact tccaaaacgg tgatggaatt gacagaaaag ctgatcactg agcagaatct
    53221 cactgctttt atggtcactc atgacatgga agacgcgatc cgctatggga gtcgactgat
    53281 catgctgcat caaggaagga tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac
    53341 agtgccccaa ttattagcgt tgttcaaaca aaacagcggc acggcattaa aagacgatca
    53401 agtactttta tcttcataat cagaaagagg atgggacaaa aaatgaacgc ccaccctctt
    53461 cttttttctc tttccttctt tcgtttttgc tttatgtatc aaccgttgtt tcttcgaaaa
    53521 ctattttgtt ctctccttct tcctgataga atggatggga aaggagcttg atgccatgac
    53581 aacgaaagac aaggtacttc aattattgaa aaagacacca gattttcttt ccggagaaaa
    53641 gttggcacag caactggaag tttcccggac aagtatttgg aaagccatca aagaattgga
    53701 aaaggcaggc tatcgttttg agcacgggcc tacagggtat cgttacctgc cctctgacgt
    53761 actagatgca aaagaaatcc agcaagagct acggtcgttt attcctgatt tgaaagtaca
    53821 aacgatgatg acttctgaat ctacgatgaa agatgccaaa ctggcagcag ctcaaggaga
    53881 ttctgtttcg aagttatttg ttgctgatac acaagaacaa gctcgtggac gttttggacg
    53941 gccttttttc gctgaacctg gacgaggaat ctacatgagt ctcttgctcc atccagacaa
    54001 acggtttgat gaattgcctc aatatacgat catcgcagcc agtgcctgtg tgaaagccat
    54061 tgaatctgta accggtcgtt ctgcttcgat caaatgggtc aatgatatct acatggatgg
    54121 aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat atggaaagcg gcaggatcag
    54181 tcatatcatc attgggatcg ggcttaattt ttctgttcct caaaatcatt ttccagctga
    54241 tctccaagaa aaagccatct ctgtctttcc cgacggacaa gctagtgtca caaggaatca
    54301 gctaatcagc aaaatctggc aagaattgtt cacttatctt tctcacccag aaaaagcgtt
    54361 ggacttctat aaagaaaaat cattcgtatt gggaaaaacc gtgtcgttca atcaaaaagg
    54421 aacgacttat gtcggtcttg caaaagaaat cggtgatcat ggcgaactga tcgtccaaac
    54481 aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt ctcacatcta tccgtacgca
    54541 ataattatca gatttcacaa aaggacacct gatctcatac agtgtccttt tttctgaatt
    54601 ctcttcaaaa gactctcctt attctatcag ctgacagttt ctctatcctc tagaaaaagg
    54661 tattctaaaa atatctataa agcatggtac actttcttta aatgaatcaa tgcaaaaaac
    54721 aagagagtca tcagtttaca cgatcaaaag agatagcagg ccgcttcctt tttcaatctt
    54781 atagatagac catgggtaac gataaaaaca aaaaagaact tgcgacttat tttgccgtaa
    54841 gttctttttt agctagtgcc ttaatctctt aatcccaaca aacgtctcgc atagatcacc
    54901 tgaccagagt gcatagcagc atcatcaata atagaaacga gacgcattcc tcgtgtgact
    54961 ggtggtgtcc agttctcgtc aattacttca tctagctcgg aaggttccag ccttcttaag
    55021 taagaaatag caaaatcagc agctcctcgc aagtatccta gcagatcttc tatttgatta
    55081 gcttggatct gctttgcttg ttccagacta tgcttccaat cttgttcgtt atctgctatc
    55141 gtcatcggaa accggctttc ccagtcttga ctatgccaaa ttggagaagt tcctgctaaa
    55201 tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag ccagccaagt cattgatttg
    55261 atctgcgggg ctctatcttc cactggaaaa cgattggcct cctcaataga gacgccttca
    55321 aacaaacgtt caaaccgatc gtatgcccga tccacactct cgcatagcat atctaaataa
    55381 tccatttatt ttcctccttt ttatttatcc tacttacttt ttcctaattc taggatacct
    55441 acttatatgt tactacacac ccttattttt tacactttaa cagtcaccat catcaatagt
    55501 ttggagattg aaagaaaaag atagtgtcga actatcaatt gaacaagaag caacaatatg
    55561 aacgtttcat taatattctt taaataaaca atgatttttc aaacgaacag tttaagacta
    55621 ggagaaaaag agtataatag gaaaatgttt gaaaggaaag gaaggggaaa aatgaaaaaa
    55681 agggcaagtt tactagtcct agttgctttg ttgttcaata tcttttcgta tggtgtggcc
    55741 gtttcagcgg aaacatacat tcctaaagac cctgggaata tttataatga tctaactggt
    55801 aactatggct tgttaggaat tgctagtcaa ttccacgtat ttgctaaaaa taagacaact
    55861 gtgaatgcac atacagacgg aaatatagca acacatgaac ttgatgcaaa aaataatttt
    55921 ggtacagagc ttcatgaagg tttattgaac caagaaataa attatataca aaaaactgat
    55981 tctttcgcta attcttcagg catacctaca ggtagcacaa gaatgaataa atttgtcgtt
    56041 ggcagtaacg tggatgtagc acttcaagat ggtcatccta cagtcaatgg atccaggctt
    56101 gatcacttaa ctccagatga tttttataaa gatcgtgctg ggcatgaata tattgatttc
    56161 gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc ttgcaacgat cactccagct
    56221 aaaacatata ctgctgcaga ttttccagac atgaacaatc ggacaatcga tttaactgat
    56281 ttatcagata ccgggttttt attggtaaat atcgatgcag aagtgttaac gatgaatacg
    56341 ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat ttaatgtcat caacagttct
    56401 tctgcattga acgtacagtc accaatcaaa tacaatgatc gttcaaatca tgaaacagaa
    56461 gatttttcgg atgcgaatat ctcttggaat tttggaaatg agatgactga tttgacaatc
    56521 agtgctcctt ttcaaggaac aatccttgcg cctaatgcta ctatccgcat tactcaaaac
    56581 caagacggaa cgattatagg aacaaatgtg attttagatg cagctaccaa tcgttgggat
    56641 cctaacgaaa tatttatcag cgacaatggt acagatacga cagacacaag cgacagtaca
    56701 gatacatcta ccacaagtga tagctcagat tcatcaactt caagcgacag tacagatact
    56761 acctctacca caagtgatag cacggataca tcagcttcaa gcgacagtac agatactacc
    56821 tctaccacaa gtgatagcac ggacacatca gcttcaagtg acagtacaga tactacctct
    56881 accacaagtg acagcacgga cacgtcagct tcaagcgaca gtacagatac tacctctacc
    56941 acaagtgata gcacggacac atcagcttca agtgacagta cagatactac ctctaccaca
    57001 agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt
    57061 gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac
    57121 agtacagata catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt
    57181 acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca
    57241 agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac
    57301 aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca
    57361 ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa
    57421 aggtaaaaaa atattcaag
//
LOCUS       DACDVT010000012        56347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDVT010000012 DACDVT010000000
VERSION     DACDVT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.012"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IWL52_000703"
     CDS             290..1135
                     /locus_tag="IWL52_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9777289.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IWL52_000704"
     CDS             1416..2636
                     /locus_tag="IWL52_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9777290.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IWL52_000705"
     CDS             2633..5353
                     /locus_tag="IWL52_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9777291.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IWL52_000706"
     CDS             5337..6281
                     /locus_tag="IWL52_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9777292.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IWL52_000707"
     CDS             complement(6350..7360)
                     /locus_tag="IWL52_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9777293.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IWL52_000708"
     CDS             complement(7540..7884)
                     /locus_tag="IWL52_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9777294.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IWL52_000709"
     CDS             complement(7877..8311)
                     /locus_tag="IWL52_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9777295.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IWL52_000710"
     CDS             8449..9186
                     /locus_tag="IWL52_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777296.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IWL52_000711"
     CDS             9179..10390
                     /locus_tag="IWL52_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777297.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IWL52_000712"
     CDS             10597..11376
                     /locus_tag="IWL52_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9777298.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IWL52_000713"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IWL52_000713"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9777299.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IWL52_000714"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IWL52_000714"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9777300.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IWL52_000715"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IWL52_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9777301.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IWL52_000716"
     CDS             14978..16027
                     /locus_tag="IWL52_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9777302.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IWL52_000717"
     CDS             16024..16302
                     /locus_tag="IWL52_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9777303.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IWL52_000718"
     CDS             16277..16747
                     /locus_tag="IWL52_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9777304.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IWL52_000719"
     CDS             16764..17066
                     /locus_tag="IWL52_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777305.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IWL52_000720"
     CDS             17083..17454
                     /locus_tag="IWL52_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9777306.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IWL52_000721"
     CDS             17426..17785
                     /locus_tag="IWL52_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777307.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IWL52_000722"
     CDS             17957..18964
                     /locus_tag="IWL52_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9777308.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IWL52_000723"
     CDS             18989..20173
                     /locus_tag="IWL52_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9777309.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IWL52_000724"
     CDS             complement(20610..22193)
                     /locus_tag="IWL52_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9777310.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IWL52_000725"
     CDS             complement(22771..22914)
                     /locus_tag="IWL52_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9777311.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="IWL52_000726"
     CDS             complement(23137..23616)
                     /locus_tag="IWL52_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9777312.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="IWL52_000727"
     CDS             complement(23689..23973)
                     /locus_tag="IWL52_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777313.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="IWL52_000728"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWL52_000728"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="IWL52_000729"
     CDS             complement(24317..25603)
                     /locus_tag="IWL52_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9777314.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="IWL52_000730"
     CDS             25741..26412
                     /locus_tag="IWL52_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9777315.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="IWL52_000731"
     CDS             26378..26866
                     /locus_tag="IWL52_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9777316.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="IWL52_000732"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="IWL52_000732"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9777317.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="IWL52_000733"
     CDS             27827..28591
                     /locus_tag="IWL52_000733"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9777318.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="IWL52_000734"
     CDS             28824..31037
                     /locus_tag="IWL52_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9777319.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="IWL52_000735"
     CDS             31048..31494
                     /locus_tag="IWL52_000735"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9777320.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="IWL52_000736"
     CDS             31665..32924
                     /locus_tag="IWL52_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9777321.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="IWL52_000737"
     CDS             complement(33013..33960)
                     /locus_tag="IWL52_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9777322.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFRETLTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="IWL52_000738"
     CDS             34354..35646
                     /locus_tag="IWL52_000738"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9777323.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="IWL52_000739"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="IWL52_000739"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9777324.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="IWL52_000740"
     CDS             37539..38414
                     /locus_tag="IWL52_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9777325.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="IWL52_000741"
     CDS             38680..40563
                     /locus_tag="IWL52_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9777326.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="IWL52_000742"
     CDS             complement(40638..40922)
                     /locus_tag="IWL52_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9777327.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="IWL52_000743"
     CDS             complement(40912..41646)
                     /locus_tag="IWL52_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9777328.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="IWL52_000744"
     CDS             41775..42398
                     /locus_tag="IWL52_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9777329.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="IWL52_000745"
     CDS             42590..43711
                     /locus_tag="IWL52_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9777330.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="IWL52_000746"
     CDS             43718..46846
                     /locus_tag="IWL52_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9777331.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="IWL52_000747"
     CDS             46865..50059
                     /locus_tag="IWL52_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9777332.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="IWL52_000748"
     CDS             complement(50205..50912)
                     /locus_tag="IWL52_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9777333.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="IWL52_000749"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="IWL52_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9777334.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="IWL52_000750"
     CDS             52407..53060
                     /locus_tag="IWL52_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9777335.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="IWL52_000751"
     CDS             53286..54284
                     /locus_tag="IWL52_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9777336.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="IWL52_000752"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="IWL52_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9777337.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18537 a         9724 c        11680 g        16406 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaag tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 atttttt
//
LOCUS       DACDVT010000013        46676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDVT010000013 DACDVT010000000
VERSION     DACDVT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.013"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="IWL52_000753"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="IWL52_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9777338.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1122..2498)
                     /gene="trmFO"
                     /locus_tag="IWL52_000754"
     CDS             complement(1122..2498)
                     /gene="trmFO"
                     /locus_tag="IWL52_000754"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9777339.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2467..4545)
                     /gene="topA"
                     /locus_tag="IWL52_000755"
     CDS             complement(2467..4545)
                     /gene="topA"
                     /locus_tag="IWL52_000755"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9777340.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4667..5524)
                     /gene="dprA"
                     /locus_tag="IWL52_000756"
     CDS             complement(4667..5524)
                     /gene="dprA"
                     /locus_tag="IWL52_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9777341.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5580..6347)
                     /locus_tag="IWL52_000757"
     CDS             complement(5580..6347)
                     /locus_tag="IWL52_000757"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9777342.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6340..7200)
                     /gene="ylqF"
                     /locus_tag="IWL52_000758"
     CDS             complement(6340..7200)
                     /gene="ylqF"
                     /locus_tag="IWL52_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9777343.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            7460..7858
                     /locus_tag="IWL52_000759"
     CDS             7460..7858
                     /locus_tag="IWL52_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9777344.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            8095..8637
                     /locus_tag="IWL52_000760"
     CDS             8095..8637
                     /locus_tag="IWL52_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9777345.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(8720..9271)
                     /gene="lepB"
                     /locus_tag="IWL52_000761"
     CDS             complement(8720..9271)
                     /gene="lepB"
                     /locus_tag="IWL52_000761"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9777346.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(9423..9650)
                     /locus_tag="IWL52_000762"
     CDS             complement(9423..9650)
                     /locus_tag="IWL52_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9777347.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(9647..10165)
                     /gene="msrA"
                     /locus_tag="IWL52_000763"
     CDS             complement(9647..10165)
                     /gene="msrA"
                     /locus_tag="IWL52_000763"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9777348.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(10185..10898)
                     /locus_tag="IWL52_000764"
     CDS             complement(10185..10898)
                     /locus_tag="IWL52_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9777349.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(10901..11797)
                     /locus_tag="IWL52_000765"
     CDS             complement(10901..11797)
                     /locus_tag="IWL52_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9777350.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(11955..12797)
                     /locus_tag="IWL52_000766"
     CDS             complement(11955..12797)
                     /locus_tag="IWL52_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9777351.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            12929..13582
                     /locus_tag="IWL52_000767"
     CDS             12929..13582
                     /locus_tag="IWL52_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9777352.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(13645..14172)
                     /locus_tag="IWL52_000768"
     CDS             complement(13645..14172)
                     /locus_tag="IWL52_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9777353.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(14192..15139)
                     /locus_tag="IWL52_000769"
     CDS             complement(14192..15139)
                     /locus_tag="IWL52_000769"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9777354.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(15151..17037)
                     /locus_tag="IWL52_000770"
     CDS             complement(15151..17037)
                     /locus_tag="IWL52_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9777355.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(17197..19275)
                     /locus_tag="IWL52_000771"
     CDS             complement(17197..19275)
                     /locus_tag="IWL52_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9777356.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(19288..19563)
                     /locus_tag="IWL52_000772"
     CDS             complement(19288..19563)
                     /locus_tag="IWL52_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777357.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(19925..20380)
                     /locus_tag="IWL52_000773"
     CDS             complement(19925..20380)
                     /locus_tag="IWL52_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777358.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(20534..21742)
                     /locus_tag="IWL52_000774"
     CDS             complement(20534..21742)
                     /locus_tag="IWL52_000774"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9777359.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(21854..22186)
                     /locus_tag="IWL52_000775"
     CDS             complement(21854..22186)
                     /locus_tag="IWL52_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9777360.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            22334..23209
                     /locus_tag="IWL52_000776"
     CDS             22334..23209
                     /locus_tag="IWL52_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9777361.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(23291..23872)
                     /locus_tag="IWL52_000777"
     CDS             complement(23291..23872)
                     /locus_tag="IWL52_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9777362.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(23878..25137)
                     /locus_tag="IWL52_000778"
     CDS             complement(23878..25137)
                     /locus_tag="IWL52_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9777363.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(25139..26185)
                     /locus_tag="IWL52_000779"
     CDS             complement(25139..26185)
                     /locus_tag="IWL52_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777364.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(26420..26695)
                     /locus_tag="IWL52_000780"
     CDS             complement(26420..26695)
                     /locus_tag="IWL52_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9777365.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(26938..28248)
                     /locus_tag="IWL52_000781"
     CDS             complement(26938..28248)
                     /locus_tag="IWL52_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9777366.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(28430..29659)
                     /gene="rpsA"
                     /locus_tag="IWL52_000782"
     CDS             complement(28430..29659)
                     /gene="rpsA"
                     /locus_tag="IWL52_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9777367.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(29794..30474)
                     /locus_tag="IWL52_000783"
     CDS             complement(29794..30474)
                     /locus_tag="IWL52_000783"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9777368.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(30523..31158)
                     /locus_tag="IWL52_000784"
     CDS             complement(30523..31158)
                     /locus_tag="IWL52_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9777369.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(31224..32627)
                     /locus_tag="IWL52_000785"
     CDS             complement(31224..32627)
                     /locus_tag="IWL52_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9777370.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(32614..33645)
                     /locus_tag="IWL52_000786"
     CDS             complement(32614..33645)
                     /locus_tag="IWL52_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777371.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            33676..33897
                     /locus_tag="IWL52_000787"
     CDS             33676..33897
                     /locus_tag="IWL52_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9777372.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(34062..34799)
                     /locus_tag="IWL52_000788"
     CDS             complement(34062..34799)
                     /locus_tag="IWL52_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9777373.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(34952..36292)
                     /locus_tag="IWL52_000789"
     CDS             complement(34952..36292)
                     /locus_tag="IWL52_000789"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9777374.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(36529..38052)
                     /locus_tag="IWL52_000790"
     CDS             complement(36529..38052)
                     /locus_tag="IWL52_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9777375.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(38430..39041)
                     /locus_tag="IWL52_000791"
     CDS             complement(38430..39041)
                     /locus_tag="IWL52_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9777376.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(39164..39289)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(39371..40087)
                     /locus_tag="IWL52_000792"
     CDS             complement(39371..40087)
                     /locus_tag="IWL52_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9777377.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(40093..40662)
                     /gene="scpB"
                     /locus_tag="IWL52_000793"
     CDS             complement(40093..40662)
                     /gene="scpB"
                     /locus_tag="IWL52_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9777378.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(40646..41443)
                     /locus_tag="IWL52_000794"
     CDS             complement(40646..41443)
                     /locus_tag="IWL52_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9777379.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(41413..41784)
                     /locus_tag="IWL52_000795"
     CDS             complement(41413..41784)
                     /locus_tag="IWL52_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9777380.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(41803..42690)
                     /gene="xerD"
                     /locus_tag="IWL52_000796"
     CDS             complement(41803..42690)
                     /gene="xerD"
                     /locus_tag="IWL52_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9777381.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(42786..43259)
                     /locus_tag="IWL52_000797"
     CDS             complement(42786..43259)
                     /locus_tag="IWL52_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9777382.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(43364..44227)
                     /locus_tag="IWL52_000798"
     CDS             complement(43364..44227)
                     /locus_tag="IWL52_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9777383.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(44423..46522)
                     /locus_tag="IWL52_000799"
     CDS             complement(44423..46522)
                     /locus_tag="IWL52_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9777384.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        12759 a         9816 c         7956 g        16145 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     1141 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     1201 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     1261 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     1321 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     1381 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     1441 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     1501 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     1561 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     1621 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     1681 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     1741 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     1801 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     1861 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     1921 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     1981 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     2041 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     2101 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     2161 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     2221 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     2281 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     2341 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     2401 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     2461 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     2521 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     2581 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     2641 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     2701 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     2761 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     2821 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     2881 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     2941 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     3001 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     3061 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     3121 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     3181 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     3241 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     3301 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     3361 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     3421 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     3481 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     3541 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     3601 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     3661 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     3721 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     3781 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     3841 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     3901 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     3961 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     4021 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     4081 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     4141 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     4201 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     4261 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     4321 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     4381 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     4441 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     4501 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     4561 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     4621 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     4681 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     4741 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     4801 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     4861 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     4921 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     4981 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     5041 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     5101 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     5161 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     5221 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     5281 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     5341 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     5401 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     5461 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     5521 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     5581 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     5641 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     5701 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     5761 cttgcagcag caatcgacac agatcgtaca tcccctttga ttattttttc ctgctgaata
     5821 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     5881 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     5941 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     6001 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     6061 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     6121 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
     6181 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
     6241 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
     6301 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
     6361 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
     6421 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
     6481 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
     6541 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
     6601 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
     6661 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
     6721 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
     6781 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
     6841 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
     6901 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
     6961 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
     7021 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
     7081 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
     7141 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
     7201 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
     7261 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
     7321 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
     7381 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
     7441 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
     7501 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
     7561 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
     7621 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
     7681 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
     7741 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
     7801 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
     7861 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
     7921 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
     7981 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
     8041 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
     8101 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
     8161 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
     8221 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
     8281 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
     8341 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
     8401 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
     8461 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
     8521 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
     8581 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
     8641 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
     8701 ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
     8761 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
     8821 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
     8881 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
     8941 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
     9001 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
     9061 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
     9121 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
     9181 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
     9241 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct ctttttttca
     9301 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
     9361 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
     9421 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
     9481 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
     9541 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
     9601 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
     9661 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
     9721 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
     9781 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
     9841 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
     9901 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
     9961 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    10021 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    10081 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc
    10141 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    10201 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    10261 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    10321 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    10381 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    10441 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    10501 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    10561 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    10621 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    10681 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    10741 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    10801 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    10861 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    10921 ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    10981 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    11041 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    11101 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    11161 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    11221 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    11281 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    11341 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    11401 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    11461 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    11521 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    11581 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    11641 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    11701 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    11761 acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    11821 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    11881 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    11941 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    12001 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    12061 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    12121 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    12181 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    12241 cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc accttttacc
    12301 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    12361 tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    12421 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    12481 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    12541 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa ttctccaatt
    12601 ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat aaatctttcg
    12661 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    12721 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    12781 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    12841 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    12901 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    12961 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    13021 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    13081 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    13141 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    13201 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    13261 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    13321 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    13381 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg gtgttatttt
    13441 gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct tgaattctat
    13501 ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt
    13561 ctctgtttta ttctttactt aataaaaaaa ataaagctat ggatgggaca gctctaaata
    13621 acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc tttttacagg
    13681 attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt catccatttc
    13741 tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa aataagcatc
    13801 tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg gcagaaattc
    13861 ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt tttccagagc
    13921 aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg aagaatctct
    13981 cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt cgaatgtttt
    14041 tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca gatcatttgg
    14101 caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct gtgcccaaat
    14161 tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct ttgatcgccg
    14221 gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat actgactgct
    14281 tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat tgttctttca
    14341 tttgttcgat atgattgata taaagatgag catctcctaa tgtgtgaaca aattcgccaa
    14401 ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt gcaatgttga
    14461 acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt agccgtccct
    14521 cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta ggaacatctt
    14581 ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg attgcttcga
    14641 tcacttgttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga cgccattgat
    14701 agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct
    14761 tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga tctgtcaaac
    14821 atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg atgtagcgtt
    14881 caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg atattcgtat
    14941 ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg
    15001 ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat aagcttaatg
    15061 tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt cttcctaagt
    15121 caagatacgc ttgttccatc ctttttcctc ctaatcttct acccattcac tcaagtattc
    15181 ccaccgtgcc attttttctt caagttgtgc ttctgtttca gatacgtcgt tttgcaattc
    15241 ttgtaatcga gtgaaattat ctccttgatg attcatctct tcttggagca atgagatttt
    15301 ttcttcaagt tctgcaatct catcttcaat cgtttcccat tctttttgtt ccatgtaaga
    15361 taatttcttc ttttcagcag gttccttgct ctgtattttt ggtttcgctt tttcagcagg
    15421 ctgctgggtc tctttttgct tcagtaaata actggacatg tttccgtaat aaagttcttg
    15481 ttttccgttt ccttggaata ctaataactt atctgctacc ttgtccaaga aataacggtc
    15541 atgggaaaca gtgatcacag cacctggaaa acttctaaaa taatcttcca aaatcgtcaa
    15601 tgttgctatg tccaaatcat tggtcggttc atccaataat aaaacattag gttgctgaat
    15661 cagcagtttc aacaaaaata accggcgttt ttctccgcct gaaagtttgc ctatcagtgt
    15721 cccatgcata aatcttggaa ataaaaatcg ttcaagtaat tctgctactc cgatttgact
    15781 gccatctgct gtttttgctt cttcagcagc ttcttgtaag taagcaatca tccgtttatc
    15841 tggtgccatt tcttcatttt cttgtgtata atacgcaaga tgaaccgttt caccgataga
    15901 cattgtaccg ctatccaggg gaattctgcc agctagaata ttaagcaatg tcgattttcc
    15961 cgcaccattt ttaccagtaa tacctagtct ttcccgagat tgaatcaata aatctaaatg
    16021 ttctagcagt gtttggtcat ttatacggta actgccatct ttgatttcca gtactttttt
    16081 ccctaaacgt tgtgtcgcta ggttcagctc taaatcatct tcttggttta cttggtacaa
    16141 gttctccttt agatcttcga aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg
    16201 tacgccagcc ctcatccaag ctaattcctg tttataaagc cgcttgcgct tttcttcttg
    16261 ttctacttct acacgatcac gttctgcttt ttctaagacg taagcttcat aatttccttt
    16321 atactcgtat aattttccaa atgaaagttc gaagattcgg tttgctactc tatctagaaa
    16381 ataacgatcg tgggtaacca tcaatagtgc gccacggtat tgtttcaaat agttttccaa
    16441 ccactcgatc gcttcataat ctaaatggtt tgttggttcg tctagtaata gtaaatcggg
    16501 ttcatcaatc aacacttgcg ccagactaac tcgttttttc tgaccacctg ataaagtact
    16561 gattttttta tctaattcac tgattcctaa tttttgcaaa ataatctttg cgttggtatc
    16621 tgtagtccaa gcatcttctt tattcatttg ttcttctgct ttagcataac gtttttgcac
    16681 atcagggtct tctccgtttt cccctagttc gatcaatgct tcctcatatg ctcgaacagt
    16741 ttggatcaag gggctgtttc cttgaaagac tgcttggaga acagttaatt catcggaaaa
    16801 ttcagctgct tgagaaagat aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg
    16861 atcaccgtcg ctgctatcga taccggcaat aatatttaag agacttgttt tacccgtccc
    16921 gttcgtccca atcaacccga tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa
    16981 caaatctttt tcaccataag tttttttgag gtcagttact tttaattctt tcaacatgtt
    17041 ccctccttat cttggatatt gtatcataaa aatagtgaac ttcaggaaaa tttttttggt
    17101 atttgttctt tttgtgttct caaaaaaaga acaggaccga ttcgatcctg ttctgatatg
    17161 ttttgttttt tataaaactt gaaaattttt acctttttat tttattggac tcgttttttt
    17221 tgactttgct cgaaacgcaa atcggaaaga aaagttatca gatggtttgt tcatcgtata
    17281 agattctaac aaattgatat tcctgcgata gctgttcgca gaaaacgtcg tgattttttc
    17341 agcaacttcg tattcatcaa tgatttttct ttcaagataa taacctctta gcattttttt
    17401 cgtaaacaga ctatcttgct ggatcatcca cgaagaaatc aaattccccg agttcatttt
    17461 tattccttca agtttgcgtt gctggataaa aaattgaacc atcgtttcat cataaatatc
    17521 agtcaagtta tctaaacttt caataatcaa ctgagtattt ttttgatatg tctctctgat
    17581 ttcttggata tcttcatcac taataaatgt ctgacgatca gcatatcttc tttgccaaaa
    17641 cattttcgga tggagaacga ttcgcattaa cctacggaca acaatgaacc aaaagccgat
    17701 aaaagacaaa atctggcttg tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa
    17761 acgataacca ttagaagaaa gcttgcccga acgataactt tcatccaatc catcccgttc
    17821 gatcgacaaa atcagtgctt gtatttcttg tacttcttgc ttttcatcat cggttaaatc
    17881 ttccaagtac agatcacgta tccgttcttg ataattttct atcactgctt cagtggctaa
    17941 aaattctttt gtattacgat caatttcctt tatttctttt cgcaaaactt caacgacctc
    18001 ttctaaaatc atcaaagcat tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat
    18061 aggcaagaca atgatcccta tgaccaaagt cactaagatg acacccgccg tcagaaacaa
    18121 aagcaaagac cgctggtaaa acatcatatt attgattgaa aatggcaaaa taaaaatcgt
    18181 agccaaactg actgttccct ttacaccgcc aaatgttaag atcaagatct cgttcaactg
    18241 ttttttgaac tttttactgc catctttgaa tacataaaaa agactgatag aaatgaatct
    18301 gatcacaaaa agcatgacag agattaatac gataatcgct aataaaagac cgtttggata
    18361 taaaccgtct ccccatactg gcgagaacac ttgcgtcaat tcgattccta aaaatataaa
    18421 tactaatgca ttcagtgtaa aagcaatagt tgtccatgta ctgtgggaca agctcgacaa
    18481 ttctgcatca aacacagaga ttttcctaaa accagaagct tggagaatac ctgctgccac
    18541 tgcagcaatg attccagata catcaaatgc ctctgccaat acataggcta gaaaaggaag
    18601 caaaagttcg atcagcagat accctgtgac atcttgtgct gatgcttttt cgatcaaatg
    18661 aattattttt tgtttgaacc agacaagcaa aaagccgatc aaagctcccc caatactaga
    18721 aactactaga ctcatccccg cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag
    18781 agcaaactgg aaggcagtaa caccagatgc atcattcaaa agaccttctc cttccaaaat
    18841 atgcatcgcc tttttaggga tattcactcg tccagaaaga gaactgactg ctaccgcatc
    18901 tgtcggacct aacgctgctc caaaagcaaa gcaggcagca agtgggacac ttggcaacag
    18961 gaatgataac gtcgcaccta ccccgacaag ggtcaaaatg actcctccaa atgccaagaa
    19021 caaaattgtc ccgaaatttt tgagaatcga agaaatatct gctttttctc cttctctaaa
    19081 taacaatggt gcaataatca taactaagaa caattcaggt tcaaaatcga ttgattgtcc
    19141 ccactctgtc agacctaata ccactccaag gaaaatttgg atcagtggag ctggaatcat
    19201 tggcaaaatg cggctagcta tgttcgataa ggtgatcgtt actgcaaata caataactaa
    19261 ctcagcaaat tccatcgtcc atccccttta ttttttattt tgtgtaagca tttttaaaaa
    19321 ttgttctttt ttctttaaaa ttttggtttc gactacttca acctgtgttt ggttcccctc
    19381 taattcacac tgtacacgtt ttgactccaa acgaagcaga tcatgtttca gacgttccaa
    19441 ctgacgatac atcaatatat ttcttttacg caacttacaa agtatttgac ggcggtcatc
    19501 catcgaaagg gcgccatagc gatcatgcaa cagttttttt tgttgttcaa aactctctct
    19561 cattttccag atctcctata cacaaacttc catcattata gcaaagttct ttttggatga
    19621 aaaatatttc attgtttcat cgtttattgc tagttagaaa tttccataaa tttttcaacg
    19681 cctcattcta ttccaaatag tatcagatat caagtaaaac ttaagaaaga taaaagtttc
    19741 tgacaaaagg atgtttactt tcccattcct gcctttcttg ttcttttatc acaacaaaaa
    19801 aatggggctg cgacaaaaat cttgtcacag tcccttattc tgaataaacg gtgtggaaga
    19861 gacagcctat cagtagtaat aagacacatc tctttcacag cctcattttt gcgtaaaaat
    19921 cgatttattc gaaaaattcc ccgtatacat cttctacacg ttgatctgga ttgatcacga
    19981 tatacaagtt acctgttttt tcgctgattt ttgttgattg atattgattg tctaatcctt
    20041 ctgttccttt atctagatat gggaagtaca caagatcagg tgtgccattt cggtaaagga
    20101 tttccattcg ctcgatatct tcgtatttca atgcgcgatt aaacatgccc atttccagtc
    20161 cgcctaggtt gatatcatgt gtcgcaactg cgtccccttc atggaagatt tcgattttaa
    20221 atccagcaca cggatggatt tcaacaaatt cactgccatt gattcgtcca aaacttgttg
    20281 tgatttgttt gatccaaaga tcgccgatat gacggcggtt gatcgtccag gtttctccat
    20341 ttctaaaata aaattttagc gctgtcattt ctttactcat taaaaagact cctttatgat
    20401 gtaatttatt cttgtactac ctcagtttag cacaaattta tgtaagtggt cacatcttat
    20461 gttttttctt ctaatgacgt atctgcccac tttaagtagc caacaaagct gagcagatac
    20521 agtctagtgt tttttaaatc aatttttctt tcgataaaaa atccaaaacc tgttttttat
    20581 cattttttat tctttgttct acgacggctt tttcagctaa ttgcaaagct tcactaaccc
    20641 acttaccagg tttttttcca gtttcttcta gcaagatttt accatctact gctaattcgc
    20701 ttaatgactt gataggtaaa cgatcatagg attctcctac ggcatccatc tgaggtgttt
    20761 cgtcaaaata gatcatcaat tcttctactt gcagtgcatg ttttttgcct aagaagtaca
    20821 attcgaatgg ttccaaagct ccagtttctc gttgtcgtag tccttggaca agtgcttgaa
    20881 cgtctttaat catttggtta gaacacttcc acgatttcaa aaaggaacga atctcatttt
    20941 cttttagatc cagttggctg atcaataatg cccatgcttg gctctcttct ttcaatggga
    21001 cggatggtaa ttcagcaaaa cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc
    21061 aaatgtagca ctctgtttca ataaatgcat ggattcccgc ggcccgttca tttcccaaga
    21121 gcatcttgat gaattccaca tttatacgtt ctacagatat tttacctaat agctcgtggt
    21181 tttctttgat ggcgtagaag gtttcttcct ctaatttgaa ccctagttga ctgacaaaac
    21241 gcaacccgcg catcattcgt aaggcatctt catggaaccg ctcatgagga ttgcctacag
    21301 ctcgcaagat ctgttcttct agatcttgca gaccatcgaa caaatcgatg atttctccat
    21361 cttctttcaa agcaaacgca ttgatcgtaa aatctcttct tttcaaatct tctgccaacg
    21421 aacgaacgaa ttcgacatga tctggtctac ggtagtcctg ataagttgat tccgtccgaa
    21481 atgtggtgat ctcatattgt tcatcatttt ctaataccaa cacagttcca tgttcgatcc
    21541 caatatctat cgtttttgga aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga
    21601 tatccacatc atggattggc tggttcagca agatatcacg aacacttcca cctacaaaat
    21661 aagcttcata tcctgcattc tctaattttt tcagaactgg aattgccttt tggtattcca
    21721 gaggtaattg ttttaatttc atttttccaa cctttcctgc gtgtttcttg tcctgagata
    21781 aagactcagt tgcatggtga ctgtttgaca agtccgtttc agagtcttca aggccaggac
    21841 cacttcatct tgtttactcg tccttacgct caaagcgatt atggtctctt tgattgaatt
    21901 tttccatgtt tttttcaaaa acttcagtta agtcgatatt caatgaatta gccatgatca
    21961 tcgtaacgaa taaaacatca ccaagttctt ctgctaccga gttaactggt tcagaagcct
    22021 ttttgctttt ttctccataa gtgtggttga cttctcttgc caattcacct acttcttcag
    22081 ttaatcgagc catctgagct aatggagaga aataccccac cttgaattgc tggatatatt
    22141 catccacttc ctgctgcata ctgtataaag aacgcttttt atccatcttt ttccctccaa
    22201 aactatctta ccaaaacttt aacgcaaatc ctagataaga gcaattgaga aaatcgaatt
    22261 tccatgctac atttaggaag gaatttctaa atggaaattc tgtgaaaata cataattgaa
    22321 agctggaatt catatggtca aacgaaaaac agtaaaagaa gttcttttta tcattatagg
    22381 aacaagtatt tatgcatttg gtcttgttta cttgaacatc gctaatcatt tagcagaagg
    22441 gggagtctca gggatcacct tgatcttaag agctttattt ggtattgatc cagcttatac
    22501 gactttatta atcaacatcc cattgatttt actaggagga aagatcctcg gtaaacgttc
    22561 cttggcctat actgttctag gtacactttc tttgtcattc tttttatgga tctggcaaag
    22621 ggttccacta gaaatcaatt tacagcatga tctactgatc gtttctctac ttgcgggttt
    22681 agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga
    22741 tattatcgct cggattttag aacaaaatta cggcatcact atggggcgtt ccctattggc
    22801 atttgatatc atcgtcttac ttgcttcatt aacctatatc gacttgaaac gtatgatgta
    22861 tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat tcgatcttag atggcggata
    22921 ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa gagattgcac cactactgat
    22981 gaccggtctg caaagaggtg tgactttcct ttctggagaa ggcgcattct ctggtaattc
    23041 aaaaaaaatc atctatatgg tcgttagctc tagtgaatta actgaagcta agaggatcgt
    23101 ccacgagatt gacgaaaaag ctttcctttc aatcatcaat gtgcatgaag tggaagggca
    23161 aggctttact tatttgaagc cacagacaag attactgaaa aaactataag aaaaaagaca
    23221 aagataggct atgaggaaag attccacatc cagctgacct ttgtcttttt tagtactata
    23281 ctatttttaa ctaattgtta tgttcactta cccggttact tttcttttgt gtctgcaaaa
    23341 ggatctttcg gttaacttca tcagataatt tcaaaaaagc atccctatct ccatcttcta
    23401 gtgcttgatc gatttgagca taaagcattt ttagttttgc ctcttcttct ttttcttgaa
    23461 aatatcgttc aattaattca gtatcttctt cactgactga agaatcccaa gaagaaaatg
    23521 gattgtcttc taagatagat agatattctc ttgtctgcca agcattttcg aatatacatt
    23581 ccacatataa aggctttttc caattcaaac ggatttcatg aaaaatctgg tctgtgtccg
    23641 taaattcttt tccctgcaaa aacaatttca tgggttcccc ttcaacagat agatcactga
    23701 tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa gtggacattg cttaaaatcg
    23761 cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc tcttcggcta aaagatacat
    23821 gattgaccat ccacgttaaa aaattcttct tttcccgtac atcaattagc atttttctca
    23881 cctctctgtt aggtcatcta ataatgacag tgcctccata tctcctggtt caagtgttaa
    23941 ataataagtc aataattctt gcgctctttt cagctgccct tcttctctta agaaaaaggc
    24001 atactctttc aagaaatctg gttcatgtga tagttcatga taagcctgtt catagtgtac
    24061 agctgcaacg gtaaagtctt ctaattcatt ataagcatgg gctaggttcc attctgcata
    24121 aggattagca gtttcttcca tctgattgat cacttgtatc acatcttcat atctttcatc
    24181 atccaagtaa agattactta aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa
    24241 agctttcagt aaaagttctt ctgctcgctg tctatcatgc agacggaaag cattttccga
    24301 ggccaggtga taaagttcga cttggtaagg attttctttg attccttctt ctaacactgt
    24361 ttgtgcttct tcaatcattt cttcttcttg aagagcttgt cctagatata gatataacga
    24421 ttgataatgc ggattaactt ctcgaagttc ttccagatag ttgattgcct gctgattttc
    24481 tttcaattga agattcgtaa aagctagctg gaacagtcga tcatctgtcc tttcttcttt
    24541 cagtgcttct tctaaaggta cgatagctgc ttcgaattct ccttgcatac ttaaggattg
    24601 tcctaaacgt tcctgcatcg agattcctga aatttcattt atcccgcttg ccaacaatgt
    24661 ttcataaacc actgctgcct ctttgaaacg atcaactgaa aaatacaatt cagctagcgc
    24721 aaactggatc aatggttcat caggaagcaa cccagctgct tctttcaatt ttgcttcact
    24781 cacttcaggg attccgataa cttggtacaa atcagctatc gccaataagc tttgcggata
    24841 gtaatcactt tgcttatcca ctttttccag gtattcaaaa gctaagtcga tctcattatc
    24901 ttcgattgca atttctgcta acgggatgtt cagtccgtca ttttctgggt attggttgat
    24961 cagttgttca aaaatctgtt ttgcttcgat caaaaagcca atagccagca attcttcgcc
    25021 tagttcagct aatatatctg ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc
    25081 ttgtgacaag tcttcatctg ataaagcttg caacattttt tcgctatttg tattcatttc
    25141 aattccctct tatctcactg acgatttgtt gaaataccgt cattgttctc aataattctt
    25201 tttcgtttgg atgttggtat ccgtctatta tacctatttt ttctaaaaca agtatttttt
    25261 tgcttttttc tgccaaaaaa agaacttttt ccaaatactc catctttgaa atctgttcca
    25321 gccagtggat tggatagcca agcaagtcca gccacacaaa aaagtttttt agttggaaat
    25381 caaaaggata tgcttggaga ttccacacga aatgaaacat cattcctaaa aattttttca
    25441 tactagcaga aagtagatgg ccattttcgg tcagataaaa agcttgttca aacagtttac
    25501 cataatttga caggttttct gcctctaatt gataaaaata gatcatttct tcgatcatcc
    25561 cggcaaacgg ccgagtcgtt aattgctttt tatttggata gtttttatac agattttgca
    25621 aaaaacgatg atcacaaatc attccgcaac agataaaagt caaaaagtca atctgctttc
    25681 cgtccacctg ccgctcgctg attgtttgat cataaacgat gcgttctggc agattgatgg
    25741 tttgcagcac tacacgattt ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc
    25801 ttagagaacg gatcgataca gggatcatcc atagtgatga atcaagggtt gtatgttttt
    25861 gaaaaaaacc tgccagatcc atgatccctt catttccaaa tccgatgata aacatggatt
    25921 gattttcggg atagcgtttg acgaaatcaa gcaggttctt taattctgtt aaatgattgc
    25981 attgagtgtt ggcacaaata taccagtcat tgtttgcttg atttgcaaat aacgggttga
    26041 tcttatctgc aaacaaatca taatagcgct gattcgttaa aaataagatt ggctgatcaa
    26101 tggaaagttc tcttagctga ctgactagcg tttctccgta aaacactttc gtcaacgcat
    26161 cttctacttg ataatcaagt tccatacgca tccctcctct tcttattttt attttaccat
    26221 tagtaagcaa attctagaaa agataagagc aaagctcacc tcataacttg caaataaata
    26281 aaaattaaat aaaatacatg tgttttctat gtatgacata cggattcgga atctctgtct
    26341 cttatttatt ccttttctgc taggaaataa aaagaaaagc acttgtagcc ctagagccac
    26401 aagcgctttt tcaactgtct tatttaacag cgtcttttaa tgctttacct ggtttgaatg
    26461 caggtacttt gctagctgga atttcgattt cttcaccagt ttgtgggtta cgacctttgc
    26521 gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat taattgaact ttttcgccgt
    26581 tagctaaagc atcttgaata gttgaaaata cagcatctac tgctgcagtt gcatcttttt
    26641 tagttaaatc agtagctgaa gcaacttttt cgattaattc tgctttgttt gccatggata
    26701 gattcacctc ctccacaaga gatatgatag aaatatcatt tagttaaaac tttctgagtg
    26761 gtccagaagt ttaaaaagaa atattgatta acttcaatat tctgttatca cgatatcata
    26821 tgaaccgctt aatagcaagg tttttaccaa ttttcacatg ctttttttag tcatagaaac
    26881 gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg tcgatatggt aaaatgccta
    26941 ttttctccta cgagggataa ttcggatgga cgtcccttca aaagtaaatg cttttctgat
    27001 ttgattttct aagaaacgtt cataagaaaa atgcatcaat tcctcttcat tgacaaaaac
    27061 gacaaatgta ggtggtttga cagcgacttg tgtagcataa aaaatcttca ggcgttttcc
    27121 tttatctgtt ggtgttggat tgattgctac agcatccata atgatatcat tcaacacagc
    27181 cgaagagata cgaagattct ggttcatact tacttgttcg atcatttcag gcagacgttc
    27241 caaccgttgt ttagtcactg cagaaacaaa aacgattgga gcgtaatcaa gataccggaa
    27301 ttcatcacga atctcttgtt cgaaatcacg cattgtattg gtttctttct ttacaagatc
    27361 ccatttattc acgacgataa taattccacg accagcttca tgggcgtaac ccgctactcg
    27421 cttgtcttgt tcccgaatcc cttcttcagc attcagtacc atcaaaacga tatcagaacg
    27481 gtcaatggca cgcattgcac gcatcacgct gtatttttca gtattttcgt atacttttcc
    27541 gcgtttacgc ataccagctg tatctatcat taagaatttc tgaccacttt cagaaacgaa
    27601 gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc
    27661 tcctaaaatc gcattgatca aagaggattt accaacattt gggcgaccaa tcaaactaaa
    27721 cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag aaatacttca ctgcttcatc
    27781 taaaatatca ccaatcccta gtccatggct tcctgaaaca ggataaggat cgcctagccc
    27841 taaggaataa aattcataga tatcgtttct catttctgga ttatctactt tattgactgc
    27901 taaaatcact ggtttattac ttctgtataa aattttcgca accatctcat ctgcgtctgt
    27961 cacgccttcg cgtccgctga ctacacaaat gattacatct gcttcttcaa tcgcgatttc
    28021 tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc
    28081 gatcacgctg aattctcgac ctaaccattc accttttgca taaatacgat cacgagttac
    28141 tccaggagta tcttcgacaa tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga
    28201 cttaccgacg ttcgggcggc caacgattgc aattgttgga tttgccatat tatttcctcc
    28261 tcacaagttt ccaatctcac ttagtttaac gaatgcattg ccaactttca agcatagatt
    28321 gtcaattaaa cgaagtattt ttattcctta ttgaaatact aatcgctttt caaaagaaaa
    28381 actgagacaa aagcagctgc tttcatctca gtcacagata caaaatcaat tatttttcag
    28441 aatcatcggt tgctttttct tctacttctt ctttcaacgc atcacctaaa atgtctccca
    28501 tagtaaatcc agtattttct tctggtaatt cataagactc aacatttttt ggttcttctt
    28561 ctgaagctgg tttttcttct aaagctttga tactcaatgc aatacgatgt tcttctggat
    28621 ggatctctaa tactttcact ttcacttcgt caccttcatg aagcacttca tgtggtgtcg
    28681 caatatgttt atgtgaaatt tgagagatat ggactagccc ttctactcca gggaacactt
    28741 caacaaaggc accgaagctt gtcaaacgtt taactgttcc gtctaatact gtgccaactg
    28801 gagcttttgt atcgatttct gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa
    28861 cacgttcttg ttcaggattg atagacaaca ctttgacatt tacatcatca ttgacagcca
    28921 atacatcact tggttttgct acatgactat gtgaaatctc tgagacatgg actagaccgt
    28981 caattccgcc taggtcgaca aatgcgccaa aatcagtaag acgtgcaaca cgtccactga
    29041 gaacatctcc ctcatggata gttgaaagaa tttctttttt cttttcttct ctttcgtttt
    29101 ccactaccgc tttatgcgat aagatcaaac gattttctga tggttcgatt tcgatgattt
    29161 taaagctcaa tgttttgcct ttatagtctg aaaagtctgc aacaaaatga tcttccacca
    29221 ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac taaaccgcct ttgacaacat
    29281 ttgtaacagg tgcttcgatg accttgccct cttggaaatc tctttcgata tcttcccaaa
    29341 cttttttagc atctaaacgg cgttttgaaa gtagatagct gccgttttct ttatctttgc
    29401 cgatcgtact gataacgact aaatctaata catcaccgac ttttactact tcgctgatat
    29461 cttctaccgg caaagttgat aattcttttg ccggaacgac tccttcaact ccagcccctt
    29521 cgattccgac aatcacttgt ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc
    29581 caacttttac ttcttgtacg cttttcattg cgtcttccat tgactggttg ttctcttgat
    29641 tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa cattctgccg taagtacggt
    29701 actgttttta gtttaattga ttattcaaaa aaaataaagc gatttcgttc gttttccttg
    29761 aaaaaacaat tgtttttcat tttttttctt aatttaaaaa agaagataac cttttttcaa
    29821 aacaacagct tttattgctg caacgacttc tggaatcgat ttgccagtcg tatcgatacg
    29881 aacagcatcc tctgcttgct tcagcggaga gacgtccctt gttgaatcat aatggtctct
    29941 ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga attccttttt cttggttctc
    30001 tttgaaacgt ctttctgcac gctcacttac gcttgctact aaaaagattt tcacttctgc
    30061 attcggtaat acagctgtcc cgatgtctct tccgtccatg acaacgccac cgcgttcccc
    30121 tatttttcgt tggacagcaa ctaattcttc tctgactttt ccatgtgcag agacttcaga
    30181 aacagctttt gtgacatctg gttgacggat ttctaaagtc acatcttttg atgctatgaa
    30241 tacatgctgt ccatcttctt gttgcgaaaa agagatagga tactttttga tcagcgaaac
    30301 tagcccttct tcatctgaaa aagatacatt gttcgtaata gctaaataag ttactgcgcg
    30361 atacatggca ccggtatctg tataaatata attcaattct tttgctaata tcttggcaac
    30421 ggtacttttc ccagatgaag caggcccgtc gattgcaata ctgatttgtg tcatgatata
    30481 actccttttt ctcagaaaaa aagccgcaaa gcgcggctat tattatttaa ttcttaattc
    30541 ttgaccaggg taaagcataa agttgtttgg atctaagcca ttcaattcaa acaattgatc
    30601 agtggtgatc cctgcacgct cagccacttg ttttggacct tcgcctgctt gaacagtcgt
    30661 tgttgaaccg gctgcagctg atgaagaagg tgttgtttct gctgcattgc tgcttgaatc
    30721 ccctgcttga taagttgcct cacttgagga agtcggtgtt gtctctgctg caatactgct
    30781 ttctgacgtt acacctgtat cttgagacac agtagattct tccgcgattg tactagattc
    30841 tactgttgaa gtctcagttg actcctcttt tgttgtactt gtagaacttt ctactactga
    30901 tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg cttgatccat ttctgatcca
    30961 gaaataagca cctgctggaa tagcgataca taagaccagt aaaatcaaaa atagcgaaag
    31021 aaataatgta tttcctttct tttgttgtct ctgagaactt cttgatgaaa cttcatcatc
    31081 tgtatcgtag attggttgtt cccatggttc ttgtgtttcg ttattttctg tagattgatg
    31141 tctatctttt ctgctcactg ttttacctcc taaactctat cgtgtgtatt ataccatagg
    31201 aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt ttttaaataa atttagtaaa
    31261 atcgtttccc aatgaagttg ttcagtctta gaaaaaagct tcctctttgg cagaagttct
    31321 gcttttgctc catcaatatc acaacaattc tgcggctttt cggagagttt ttcgccaaaa
    31381 taagctaaga tgaattttct gcggcagttt tcttcattaa tatagcgaag catctgctgc
    31441 aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt
    31501 tcagactgct ggatcagctc catccatttt ttttgcagct catcaaaggg agcttgctct
    31561 gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag
    31621 aaataatgga tacgttcatc cccactttga taaaaaagaa ttgctgcact ttcttcctga
    31681 tctctgcctg ctcgaccgat ttcttgtaca tagttttcta gactgtctgg caaatcataa
    31741 tggaccacat aacggatatc agatttatcg atccccatac caaatgcatt cgtagcaatg
    31801 agaaactgca gctgattttt tacaaactgc tgttgaagtt gtcttctttg agctgcatca
    31861 agaccaccgt gatagtatcc aactgtaaaa cgttccctga ataaatgata caatcgttcg
    31921 acttcttttt ttgttgcaca ataaatgatt gccgctcctt gtgctctttc catgaagtgt
    31981 tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg
    32041 actgattgtc tgatctcttt tggtggtcga tcaaatagta catgacgtat atctcttgct
    32101 actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg
    32161 atttttccta actgttgata ttctggacgg aaatcaactc cccattgaga cacgcaatga
    32221 gcctcatcta ctacatataa cgcaatattt tgttttttca gatgctctaa aagagaagga
    32281 ttagtcaaca tttcgggact caaaaacaaa aatttatatt gcgatagatg acggaggacg
    32341 tatcttcttt cagattgcga aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt
    32401 ttttgtaatt gcgtcacttg atcttccatt agagaaagca gcggtgacac gatgatgacc
    32461 agaccttcca ttaaataccc cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc
    32521 aaaatagcga gagtatcttc tccatcaagc aacgcctgaa taatttcttc ttggccggga
    32581 cgaaatgaat caaatccaaa atattttttt aattcattta ttaacattct gtatcgcttc
    32641 ctttctcaat agatagattt gatacaaacg aaattctcca taatctaatt gtccatctac
    32701 attccattcg ctaaagcgcc actctcgcac ggaatcctta tttggttcca aacgttcgat
    32761 tgtttctgca gagagaatcc actcgtatgg aaaatcatca aaaaacaagg cccactcaat
    32821 aagatgatct gttactgttc ctctttttaa atgacggatt gccatgatct ggtcaattgt
    32881 ctgaccattc ataaacatct gcttggtgat catcatgctt tgattgaaat tttgctgtaa
    32941 taatgaatcg atcaaagcgt gccaaagcga atctggccgt ttttcgattt gtgctaacaa
    33001 aaggtggata catctgcttt gttgcagata aaccgctgac tcatcattgt tttttgccag
    33061 ctgataagga agcagccctg ttcgctcatt tccagataat tggttagcta aaaaatctgc
    33121 tgcttcttct ggtatctccg aaaacagctg caccaaactc tcatacgcag tatcaatcaa
    33181 aatttcacgt ggcaattggc tttcactaag ccattttttt agttggaaag tataaaaagg
    33241 acttgtctct gctggaagat agtcttgaat acccgttgcc agattagaaa ttacttgaac
    33301 agcaaattta atgagccgcc aactagtttc ccctgttctg ccatagcggt caaaacgcaa
    33361 accagtatgt tctatttgat gttcggacaa catatcagcc cctgcaggcg ttagctttgc
    33421 ttcattttca tttatttcga tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa
    33481 cttatcctgc ttcaattctg gaaaactgtt atggataaac aaaagttcgt gggtaaatcc
    33541 ataaataagt actgaagtcg ttcgttttcc cacaaggagt tggtaaagag aactaatccg
    33601 cagcttgtgt cctgatgaaa atagcgataa aataaaaaca atcatattat tctccttaag
    33661 ttaggatggt tagaaatgtc actactatgt aaacttgtac ctgaacgctg tattgcatgc
    33721 ggtctttgtc aaatcgaagc gccagatatt ttcgattatg atgaagaagg aatcgtttta
    33781 tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc cggaagaaga aacatatgta
    33841 aaaaaagcgt atcaaaaatg tccagttcga gcgattttat tagaaaataa aatataaata
    33901 taaacgaaac tttaaaaacg tacactaagt tctctaaact tgaaaataag ccgggatctg
    33961 tttctcttgt tccgtgaaat cccgacttat tttgaaagat tcatttgcta tctatagtta
    34021 attgactagt tttttcacgg accctatagg acccatttat attatttcaa ctttttcagc
    34081 cagttttcca atgtattgac gatcacagtt tgctggtctg cgttagaaat ttctgcctct
    34141 ccatctcctt tttgctcacc atagctgcca aaacctccat gatttccgcc attgattgat
    34201 acatactctg ttctttccgg taaatactgc ttagcttctt gataagcttc ctgattcaat
    34261 actttatctt ttgtcgccgt gatcgataaa acaggaacag ctgattctgt caatgttcct
    34321 tttttgtcgg gataacttgc catgaagaat acccctttta aatgtttgtc tgaatgattc
    34381 ttattgacat aacgacttgc cattacgcca cctaatgaat ggccaccgat cacataatcg
    34441 gaatttggaa aatcttcttt gaccttatcc gctttgtttc cgcccatcac tgctagatca
    34501 agtggcattt tgacaatagc gactgaaaag ccgttttttg ctaaatcttg cgcccaaata
    34561 ctataacttt ctggctctac gagagcacca ggataaaaaa taagtaatgg agactttcca
    34621 tgattctttt tctctccttc aaaaaataag taatcttctt tgttttttcc ttcttttacc
    34681 aacacttgcg cttctgacga tggttcgtac gtattttgct gtatatagta ataaccggct
    34741 ccaccgagta atcctaaaat gatgactaag ccaattagta ttcgtctaaa tattttcact
    34801 ttctcccacc ttttctatct gagtgacccc tttttcttaa tcaagtatga ttatacctta
    34861 aaaaaataag agagaaatgc aaactttcca gcatcaaata aaaaaagacg gaatatccat
    34921 ttgaatatcc gccctttttg tatgtttatt tttatttagc acataaacga tacacagctt
    34981 ctgcataaat ttccattgct ttatacatac tacttaattc ccatcgttca ttcgcctggt
    35041 gcataaagtc aggagtatca gggaacatcg caccgaatgc cacacactgg ttcatcgtac
    35101 gagcaaaagt agcgccgcct gatacaaacg gttcagtcat gtctccagta atatcgcgat
    35161 atgtccccaa tagatttttc actaactcac tgtctagtgg cacgtataat gaatcaagga
    35221 agtcaaattc ttcataagtc aacccatatt tttctgctgt tttagttaat ttagctacta
    35281 aatcatcttt cttgaatgta acagggattc tcatatctac accgattttt gtttgttctg
    35341 gtgtgatttc caaacttgca aagttcatcg ttagctcgcc ggattgttca tcttctgttt
    35401 tgcccacgac agcttcacca gttgcatttt cttgaactaa ttttcctaag aaattgattg
    35461 ggccaaaatc aaatacttct gaaagagcaa ttgccagacg agaaatagca ttcactcctt
    35521 gtgctgcatc tttcgcgtgg atacttttac caagaacgac gatgccttca ccttgttctt
    35581 cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc agaaagcttt tctccagagt
    35641 aaggagctgc atcaggaaca acattcaacg ctcctccagc ttttacagaa aactcgttcg
    35701 ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc tgcataaatt aaaggaaatt
    35761 cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt ttcgttatat ttttctaaac
    35821 aacgccataa attttcttca tctgtaccaa agataaaacg aatacgtgtc ttgaattcca
    35881 cgccagcatc aatcaaagct ttgacggcat acattgccgc aatagaagga cctttgtcat
    35941 cttgggatcc gcgaccaact aaccagccgt cttttatagt tggatcgaat ggttttgttt
    36001 cccaattatt ttcgtcccct gctggaacaa cgtccatatg acacaagata ccgaataatt
    36061 catctccaga gccaatctct gaataaccgt aatagccttc tggatcttta aatgttctaa
    36121 atcctagctt ttcactgatt tccaatactt tatccaaagc ttcaatcact tttttaccga
    36181 agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga aggaattcgg atcaattcgc
    36241 tcaatgattc taatgcttgt tcatgatgtt gttctgtcac aaattttttc atgatattat
    36301 cctcctaata tgttaaaaag ggtcatgtca aattcctccg acacaacctc ttcttctcaa
    36361 ataatgacgt tttaagcctg ctccatcagc aagcagaatg ttcttaacaa atgatgaaag
    36421 aaaaaacact cactgaatgg agctattgac ttatccacgt gcttatcgat agccgtttat
    36481 cggtttcttc attcttatga tagaccttgt acctgtattt gtctaggttt agaacattgc
    36541 tgctacacct aggatcacac agatggctac aaataatatc gcaattaatt taccggtaaa
    36601 tttccaccac actgaaacat cgaaacgagc aattgctaca gcacccatta cgatagcaga
    36661 tgttggtgta attaagttta ctaaaccaga tgcagattga taagcagtga ttactaaatc
    36721 cttaccgacg ccggcaaatt cacccatagg tcccatgatt cccattgttg cagcagctaa
    36781 gccggaagtt gaaggaatca agaatgacat agggatatag aaaataaatg taataatgat
    36841 gaatacactt gaagataatg aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt
    36901 gattaatcca ttgttcataa ccacttggat accacgagca acagctacta cgattgctac
    36961 accgatcaaa tcagaagcac cagctaaaaa ttcagagaca aattcagact ctttcatgcc
    37021 ataagtgatt gctactacga tggacattac taagaataac atagtaattt ctgggaagta
    37081 ccagtcacct aatggaagca tgcttttacc tagaaccgta cctagtaccg gaaggtttgt
    37141 taaccagttt gtcaatgatt caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat
    37201 caaactgatg atcatgatac caaaagtaat aaagaacaat acattaacat gtttttgttt
    37261 tttcgtgatt acttcttgac gaccgattgc ttcaatatcg aaatgcttca tatcttcttt
    37321 acgttggttg tatactaatg attttgttgg atctttttcg atctttgatg cataacgata
    37381 cacatataca atagaaatag ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc
    37441 ttcacccatc gaaatcccaa cagcttgtga agctacgcct gttgcaaaag gatttaccgt
    37501 tgaagctaga cagcctactt gagaccctac taatacgatt gctacagcta ctactgtatc
    37561 aaagcctaca gccatcatga ctgggatcag taaagcatag aatggaatcg tttcttctgc
    37621 cattccgtaa gtagagccgc ctaatgcaaa taacagcatc aatactggaa taagcatttt
    37681 ttcttttcct ttgaatttct tcacaactga agcaatcccg gcatctagtg cccctgtttt
    37741 gttgataata cctaaaaatc caccaacaac caaaatgaag aaagagattg ggattgcagc
    37801 aggtgtttct ttagttagct cccccgctgg tacacctagc atcccattga ttggcgccat
    37861 gaaaatatcc cacagccctt gaggattttg ttgtactcgt tcatatgatc cagcgacgat
    37921 gttgcctgca tcgtctacac tatagtgccc agctggtata aaccaagtaa gtacagcgat
    37981 tactgcaata ataataaata agaccgtata cgctgaaggc atttgaaatt ttttcttttt
    38041 tgtttgttcc atcttcttct tcctttcttt tccgttagag acaacaattt tggctctaat
    38101 gttattttat caaaaagagt atcagaacaa aaataataac acgcattttg taaaactttt
    38161 gttcaaaaag ttcgtttttc cctattccct cctctttttt caggttttta aagctttctt
    38221 ctttttcatt gtagcgtttc ctttttttta taaacaaaca ttttcgtctt ttaaaaggag
    38281 aagaatttca catttgcaaa gaattgattg atttttatgc ataaatcaaa aaaatactca
    38341 aaacaaacaa ataaaacgtt tacattattg catgtcttgt atttttttcc aaaaaaaaag
    38401 accaagaaat ttcttgatct ttttgatatt tatttgactg ctgtatgacg ttgttcgaac
    38461 tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat gcagcactaa aaagacagca
    38521 ctcacgatga atcctttgat caagttgaaa ggaacgatcc caatcagtac atagtttgct
    38581 aaagacatgc ccagcatctg tgttgcgttc agccctaaaa acattaagta aagaggcgta
    38641 ataacgaaat aatttgcgat actcataaaa atagttagag cgatggttcc tgtaatgaca
    38701 cctaaaaatt tgtttcttcc tttatgctgt ttctttacac taaagaaata ataaatcggg
    38761 aatgtaaaga tcgttgttga taagaaacta gcagtgtctc ctaccaaatt atccggagaa
    38821 aaacctgtag tcagcagatg taatactgaa cgcaagaacg ctgttgcaac tccggccgct
    38881 ggaccgaata agaacatgct gatcaatacc gagatatcac taaaatcgat cttcaaaaaa
    38941 ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac
    39001 atcgctactg caaccatttt ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg
    39061 actcctcttc agcgaacttt ttgacgtttc tctccaaatg aaaaaactct attttccagg
    39121 gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc
    39181 agactatact gtcggtatcg gaatttcacc gattcagcta tttttttgca aaataggtcg
    39241 tggactataa ccaccggtcg ggaatttcac cctgcccctg aagacgaacc ttatataatt
    39301 tttgtttaca tattcattat acatatctat tcaaaaaacg caacatctaa cacttataaa
    39361 aagtaagtgt tcattttgag tggttcagca aactgctgac ttcttttttg ttcagttcac
    39421 ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc gtatttttca cgttttagtt
    39481 tttgcacagg aagaccaact gcttgaagca tatttttcac ttgatgatta cgtccttcgt
    39541 ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc cactgataaa atttggaatt
    39601 tagctggaga tgtttttcgt ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg
    39661 attttgtagc gatccctttt acctttgcaa cgtaaacttt atccacttca tgttttggat
    39721 gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag caagatcccc gaagtatcgt
    39781 aatccagtcg acctacagga tagatacgtt cttttaccat cggcaaaaga tccgtaacaa
    39841 cttttcttcc tttgtcatct gcaacagcag aaataactcc tttaggtttg tacaataaat
    39901 agtaaccgta ttcttcttga taaattggga cttcatccac ttccacagtg tcatgtttcc
    39961 caacttttat ccctaattcc cttacaactt gtccgtttac ttttacacgt cctgcaagga
    40021 tgaattcttc tgcttttcga cgagaagtga tgccagcgtg ggcaattact ttctgcaatc
    40081 tctccattac aattattctc ctttattcat tccatcaaag aatagatctg gtgacacgtc
    40141 ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt tcttctaatg atttcaaacc
    40201 aaaataatct aagaagtagc cagttgttcc atataatatc ggccgacctg gtccttctac
    40261 tctacctttt tcttcaatca gtttatgggc agctagacgc tgtaccgctc ctgaaacctg
    40321 aactcctcga atctgctcga tttcagctct tgtgattggc tgtttataag cgatgattgc
    40381 taaggtttcg acggcggctt gagataatgt gtttgatgtc ccttgcgcat atcgcttaag
    40441 taagggagcc agctcttttt tagtcgttaa tacataatgg ctttccgttt caaaaatatg
    40501 caaagctgag tgcgagtttt gagtgtaaga ttcttttaat tctttcacta aacttgtgac
    40561 ttctgcttcg ctgagattta acagtccgct taattcttca ttactgatcc cttcctcgcc
    40621 aacaataaaa agaagggctt caagttcact taatttcgtc atgttcggta tctcctactg
    40681 ctggataaag cataattgat tcatagtttt ctttttgttg cgcaatgatc tgcccttttt
    40741 tcatcaattc tagcaaagcc ataaaagtag tgacaacttc acttttagaa ggtgactcaa
    40801 aaaaagaatc gaaagtacag ccatgcgcat ttctttttat acgctcttcg ataaatacca
    40861 ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt
    40921 tcttacgttc taagattttc tgcatggcac ggatcaaatc gatttttttg acttgtcctc
    40981 gcttcaacgg cttttcagca tccgctaacc catcgatgtt cataggttct tttgtgtaat
    41041 aaagacttcg ttcctctgct ttttctgtca gtctttcagc tgcatattta tatttacggt
    41101 attctaaaag ctgagtgacc aattcttctc gaggatcttc ttcccataat tcttcatcgt
    41161 ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt gattgccatc aatgtagcag
    41221 ccatcactaa gtattctcct gcaacagcta gctcgagtgt tttcattgta tggatataat
    41281 tcatatattg ctccgtgatc gtagcgatcg gtatatcata tatatcgatt tccatttgct
    41341 gaatcagatg aagcagcaga tcgagaggac cctcgaatac atctaattta atattgatct
    41401 ccgtcaactc attcactctt tcttcttttt ctttgctagc cattttccaa tgtaaggctc
    41461 tgtgatttca gttgcacgga tggagacatc cgggtacttt tcctgtaatt catctaacat
    41521 ttgaaagccc atattctcac cgcgataaga cggattcaaa gaaatgtcat ggatgatcag
    41581 ctgatcgttc gttgcccagt aaataccaat aatcccttga atgttggttg aaccttctgg
    41641 cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac gtatcaatct ccttcaacaa
    41701 agaagtttta cctgtcagtt tcttatggaa actcaatagt cccatagcaa ttttacggtt
    41761 ttctttccgg taatgtgtta acatcttctc accttcctat gactaagccc ttggaaaatg
    41821 ctgcttataa acatccgtca ttcttttctt cgtgatatgt gtataaatct gagtcgtaga
    41881 gatatccgcg tgacctaaca attcctgtac tgtccgcaaa tcagctccat tttctagcag
    41941 atgtgtggca aagctatgcc gcagcgtatg tggtgttact tctttgtaaa tcccagcctc
    42001 tcgtactaac tgcttcagat ttttccagat tccttgccga gaaagacctt tcccatgatt
    42061 attgacaaat acatgcattt cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa
    42121 gtagcgttcc agccattgga tcgcataatc acctaaagga atgatccgct ctttatctcc
    42181 ttttccaact gtctgtacca gtccaagaga aagatgaaga tctcccaatt tcaatccaat
    42241 caattcactg acgcgcatcc cagtagcata catgacttcc aagattgcac gatctcttat
    42301 ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt tctacttctg tcagagtcaa
    42361 tgtacttggt aatttctgtg ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc
    42421 agtgatacgc tcttgccgta aaaactgatg gaatcttctg agacttgaga tcatccgagt
    42481 aacagttgct gtcgcttttt tttcttcatg caacttctcc agaaaacgga tgactaaaaa
    42541 gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt agaaaatgca agtactgttc
    42601 caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta
    42661 gtggagataa tcgttgattt gctctttcat aataatcccc ctcatgcctc tctatcatat
    42721 cagataccgc ttcattctga tagtcttttt ccataatctg agcaaagaaa aagccgtcta
    42781 gacactcaga cggactggtt tcttttttct ttttcatagc attcctgaca gattccatgg
    42841 aacgttagcc gatgatcttt tactaaaaaa tgataacgtt cttctactac ttgctccact
    42901 tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc agttcaagca tagtaaatga
    42961 tgatggaaat gtttggcgcc ttcttttctc aaatcataac gggctaatcc atcattaaaa
    43021 cttactttat cgacaatttt caattctgtc aagatttcaa gggttcgata aacagtagca
    43081 agtccaattt ccggactctt ttttttcact aaaaagaaga tttcttctgc agataaatgc
    43141 tctttctcat tttccaaaag cacaagcaga gtagcttctc tttgcggtgt cagcttaaac
    43201 cccgcttcat gtagctgctg ttttgttttt ttcatcgccg aaatagcatt catgaccatt
    43261 tactcaaccc caacaattta taataattac aaataaaaaa agatataaca ctatgttaat
    43321 taaaattatt ataagctagt taagaaaaac atgcaaggga ttctcaatta gattttttga
    43381 ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg
    43441 cgcgcttaaa tgctcctttg ctgataccaa aagctttcat gatttcttct ggatccgatt
    43501 tatctgtaaa gttgatttgc tgatctgcag aacgttctaa aatggttaaa atcatttgtg
    43561 catcatctga tatagcttca tatgctcttg gcttcaatga aagattcagt acaccgtctg
    43621 gtcggacacc aatcactcga ccttttatct gctcgcctag acgaggttca ttatatcgtt
    43681 cagaaggatg gataaaccca agatagaaat cttccgttaa aacgtacgta cctgttaatt
    43741 tcaagcggta tgctgttcct atcacatctt tgtttttcat ttcttctgtt ccatatttgc
    43801 ttaaagattt gaatattttt tcatctgcta aactgcccca aatacggtct tttgcatcaa
    43861 ctctaaggga aatcatcaag cgatcccctt ttttaggcca tagctctgtc attgtcggca
    43921 attcatcaag agatacgaca aagtctttat ctggaagacc gatatcgaca aacgcaccta
    43981 aatcttttcg tgtagcagta acgattccaa aaccgtaatg accgatacgg cttttaggaa
    44041 tttgtgtagt gatccgattt tcatgttttt gattttggta accgaatcct tcaactgctt
    44101 caccgatttt gtgttcacct tctgatttgc ttaagccaaa ggtttgtccg tttttttgga
    44161 caaagtagtt tttttcattt tcatcaataa taatacctgt aaatatagtt gctaataaat
    44221 cgttcatttt tttctccctc tatagtgaaa ggttttctta atacatttct ttgctatcat
    44281 aacataatgt tatctacttg aatagctgag ccttggtttg gaaaggtgaa aaaaggagta
    44341 tgacaacagt tgtgtcatac tccccttttt cgaataaacg gtgttaaacg gcaggctcac
    44401 agcctctttc atttgcctaa aattaattgc tcccatttcc tccgaaccat ccagttttac
    44461 agttgatggt tacgatgtaa aatggcaaag cactttctcc aatgcctttc atcaatgcag
    44521 attttgttga agtggcatat agattataat agccttcccc attttcgatt ctggcttttt
    44581 ctaatctgta acctccttca acgatatatc cacgttggat acatgtagca atcactttgc
    44641 tttggacacc tggtgcccac gtccatgctg gattagatgc atcggcaata tcacttgctc
    44701 tggcagattt gattggctga ttcgctgagt ttgaagagga aggggtcgag gatgccgctt
    44761 cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc ttgagctgca gctgctgctt
    44821 gagcggcttg ttttgcttgt tcttcagctg cttttttcgc ttcttctgct tttttctttt
    44881 cctcttcttc ttttttctct ttatcagtga gcatcttatc tacttgtttc aattgttcat
    44941 tcaactgagc agatagatct gtatttttca ctttttctac tgcttctttt gccgtcttgt
    45001 attggtcgcg tgttgctgaa tccactactt ttccgtcttt aaagactata gctgtttttt
    45061 ctttagctgt ttgcaattca gcatattgat ttttagcttc tgtttgtgct tgttgaatca
    45121 attttccaaa atctgtgtta tccgtttcga ttgcttggat ctctttatct gctttcaact
    45181 tgatgtcatc tgctagatga tcatcgacga tggcaggtgc tatgaaccac tcattcactt
    45241 tttggatatc tcggatctta gattggatat cttggtaagt tgtttctaac tctgaatatc
    45301 ctttttcatt cttaagactg gataaatcat tttttagttt tgataaatct tgatgaatca
    45361 tatctgcacg aataaattga tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa
    45421 gttcttccat ttgttgctgt ctttttgctt ctgctgctac ttcggcttct actttttgat
    45481 gatggtcata ataagcccag cttcctccac cgagtagaag tactgctgct aaaacgagat
    45541 aaggtacttt ttttgatttt ttcttttgcg ctttatctgg atctgttttt tctaagtttg
    45601 attcttgatg tgttttttca gtatctggca cgattgttat tgatttcgct tctgcttcaa
    45661 acagcttagg ctggttctct tctttttcta tttttgcttc aagatcagta ccctctttgc
    45721 ttacagattg attttcagca tcggattcac tatttatttc atcgggctct gttgactttt
    45781 tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg cagtttattc gttttttcct
    45841 tttctaattg tttttcagta aatacttcag ttttttctgt gatatccgtt tttacgtctt
    45901 ttatttttac tgtttcttta cctgtggcgt tttctctatc agagtctttt tcagtttctt
    45961 gttccttttc tggctctact ttgttttcct gtgcaatttt ttcactgatc attttgagca
    46021 gctctttcgc gtgctcttct tttgtatctt cacgatgtgc tttgatataa gcggatagta
    46081 tcgaattttc ttcctcatta tcgacgattt ctttcagttc agctactctt cttttttgtt
    46141 ttgattcaag cgtttcttct tgattctttc ctgattcttc cgaagtctca tgaacagttg
    46201 ctttcttctg ttcatttttc tctatattag ttacttgatc gtgagcaggt ggttcttcgt
    46261 cttctggttc tgctttttcg aataattcca ttacagattc tagaggtaaa tctttcaatt
    46321 ccgaccattc gattggatcg tttaattctg tatcaggaaa gactgcatct tcagttgatt
    46381 tatcctgcat agctgcatct gtatttgttt cttcaattga ttcttttatt tgctgatctt
    46441 ctgcagttat ttgatgaccg cagtctggac agatagtatt ctgctccatt tttttcgatc
    46501 cgcaatttgg acattttttc atcacttagc gatcggctcc tttactaggt ttacgctacc
    46561 atttaatcta tcatattcta tacgtcacgc caatagctta tttttaggat ttacttaaaa
    46621 ataatgaatg aatttccttc ctatagaaaa aaaagcttga gactgcgtag tctcaa
//
LOCUS       DACDVT010000014        43944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDVT010000014 DACDVT010000000
VERSION     DACDVT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.014"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..378
                     /locus_tag="IWL52_000800"
     CDS             133..378
                     /locus_tag="IWL52_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9777385.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            550..1614
                     /locus_tag="IWL52_000801"
     CDS             550..1614
                     /locus_tag="IWL52_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9777386.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1860..2285
                     /locus_tag="IWL52_000802"
     CDS             1860..2285
                     /locus_tag="IWL52_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9777387.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            2278..2727
                     /gene="nusB"
                     /locus_tag="IWL52_000803"
     CDS             2278..2727
                     /gene="nusB"
                     /locus_tag="IWL52_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9777388.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2824..3678
                     /locus_tag="IWL52_000804"
     CDS             2824..3678
                     /locus_tag="IWL52_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9777389.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3679..5016
                     /locus_tag="IWL52_000805"
     CDS             3679..5016
                     /locus_tag="IWL52_000805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9777390.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            5021..5245
                     /locus_tag="IWL52_000806"
     CDS             5021..5245
                     /locus_tag="IWL52_000806"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9777391.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            5247..6137
                     /locus_tag="IWL52_000807"
     CDS             5247..6137
                     /locus_tag="IWL52_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9777392.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            6146..6964
                     /locus_tag="IWL52_000808"
     CDS             6146..6964
                     /locus_tag="IWL52_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9777393.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            7071..7520
                     /locus_tag="IWL52_000809"
     CDS             7071..7520
                     /locus_tag="IWL52_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9777394.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7538..9220
                     /gene="recN"
                     /locus_tag="IWL52_000810"
     CDS             7538..9220
                     /gene="recN"
                     /locus_tag="IWL52_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9777395.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(9275..9391)
                     /locus_tag="IWL52_000811"
     CDS             complement(9275..9391)
                     /locus_tag="IWL52_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9777396.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9464..10420)
                     /locus_tag="IWL52_000812"
     CDS             complement(9464..10420)
                     /locus_tag="IWL52_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9777397.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10591..10959
                     /locus_tag="IWL52_000813"
     CDS             10591..10959
                     /locus_tag="IWL52_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9777398.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            11129..11560
                     /gene="mraZ"
                     /locus_tag="IWL52_000814"
     CDS             11129..11560
                     /gene="mraZ"
                     /locus_tag="IWL52_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9777399.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11581..12540
                     /gene="rsmH"
                     /locus_tag="IWL52_000815"
     CDS             11581..12540
                     /gene="rsmH"
                     /locus_tag="IWL52_000815"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9777400.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12545..12946
                     /gene="ftsL"
                     /locus_tag="IWL52_000816"
     CDS             12545..12946
                     /gene="ftsL"
                     /locus_tag="IWL52_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9777401.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12947..15139
                     /locus_tag="IWL52_000817"
     CDS             12947..15139
                     /locus_tag="IWL52_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9777402.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSVYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            15165..16127
                     /locus_tag="IWL52_000818"
     CDS             15165..16127
                     /locus_tag="IWL52_000818"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9777403.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            16141..17502
                     /locus_tag="IWL52_000819"
     CDS             16141..17502
                     /locus_tag="IWL52_000819"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9777404.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17528..18616
                     /gene="murG"
                     /locus_tag="IWL52_000820"
     CDS             17528..18616
                     /gene="murG"
                     /locus_tag="IWL52_000820"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9777405.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18631..19851
                     /locus_tag="IWL52_000821"
     CDS             18631..19851
                     /locus_tag="IWL52_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9777406.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            20007..21332
                     /gene="ftsA"
                     /locus_tag="IWL52_000822"
     CDS             20007..21332
                     /gene="ftsA"
                     /locus_tag="IWL52_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9777407.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            21355..22596
                     /gene="ftsZ"
                     /locus_tag="IWL52_000823"
     CDS             21355..22596
                     /gene="ftsZ"
                     /locus_tag="IWL52_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9777408.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22613..23290
                     /locus_tag="IWL52_000824"
     CDS             22613..23290
                     /locus_tag="IWL52_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9777409.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            23304..23903
                     /locus_tag="IWL52_000825"
     CDS             23304..23903
                     /locus_tag="IWL52_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9777410.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23918..24205
                     /locus_tag="IWL52_000826"
     CDS             23918..24205
                     /locus_tag="IWL52_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9777411.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            24254..25036
                     /locus_tag="IWL52_000827"
     CDS             24254..25036
                     /locus_tag="IWL52_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9777412.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            25057..25764
                     /locus_tag="IWL52_000828"
     CDS             25057..25764
                     /locus_tag="IWL52_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9777413.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            26026..28824
                     /gene="ileS"
                     /locus_tag="IWL52_000829"
     CDS             26026..28824
                     /gene="ileS"
                     /locus_tag="IWL52_000829"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9777414.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28873..30399)
                     /locus_tag="IWL52_000830"
     CDS             complement(28873..30399)
                     /locus_tag="IWL52_000830"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9777415.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(30414..31061)
                     /locus_tag="IWL52_000831"
     CDS             complement(30414..31061)
                     /locus_tag="IWL52_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9777416.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(31245..31574)
                     /locus_tag="IWL52_000832"
     CDS             complement(31245..31574)
                     /locus_tag="IWL52_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9777417.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31751..32479
                     /gene="sfsA"
                     /locus_tag="IWL52_000833"
     CDS             31751..32479
                     /gene="sfsA"
                     /locus_tag="IWL52_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9777418.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32495..33508
                     /locus_tag="IWL52_000834"
     CDS             32495..33508
                     /locus_tag="IWL52_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9777419.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33508..34785
                     /locus_tag="IWL52_000835"
     CDS             33508..34785
                     /locus_tag="IWL52_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9777420.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34848..37550
                     /locus_tag="IWL52_000836"
     CDS             34848..37550
                     /locus_tag="IWL52_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9777421.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37702..38019
                     /locus_tag="IWL52_000837"
     CDS             37702..38019
                     /locus_tag="IWL52_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9777422.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            38049..38369
                     /locus_tag="IWL52_000838"
     CDS             38049..38369
                     /locus_tag="IWL52_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9777423.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38477..39937
                     /locus_tag="IWL52_000839"
     CDS             38477..39937
                     /locus_tag="IWL52_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9777424.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            40005..40226
                     /locus_tag="IWL52_000840"
     CDS             40005..40226
                     /locus_tag="IWL52_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777425.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            40257..40439
                     /locus_tag="IWL52_000841"
     CDS             40257..40439
                     /locus_tag="IWL52_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9777426.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            40439..40852
                     /locus_tag="IWL52_000842"
     CDS             40439..40852
                     /locus_tag="IWL52_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9777427.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40975..42156
                     /locus_tag="IWL52_000843"
     CDS             40975..42156
                     /locus_tag="IWL52_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9777428.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            42545..42618
                     /locus_tag="IWL52_000844"
     tRNA            42545..42618
                     /locus_tag="IWL52_000844"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42579..42581,aa:Arg,seq:cct)
     gene            complement(42687..43826)
                     /locus_tag="IWL52_000845"
     CDS             complement(42687..43826)
                     /locus_tag="IWL52_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9777429.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        14608 a         7457 c         9376 g        12503 t
ORIGIN      
        1 aagaaaactt ctaagaggag gttttcttta tttttttact ttgcagaaga aaatatgaaa
       61 tagcaaaacc aattatttgt cccctttaaa tcccatatgc taaaattgat tagcaaagaa
      121 ggaggttata ttatgagttg gttatggaca ttgattgttg gagccgtgat cggagcaatt
      181 gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg taacgaatat cattgctgga
      241 ctagtcggtt caagtattgg acaagcgatt ttcggcgaat ggggaccaaa agctgcaggt
      301 atggcactta ttccatctat tttaggcgca gtgattttgg tagcagttgt ctcatttttc
      361 tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg cagcgagact
      421 gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga aatttttatt
      481 taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa agaaagaggg
      541 agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga agaaaatttg
      601 gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt tacaggtacg
      661 acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt tcgttatacc
      721 gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg acctattttt
      781 gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt tgaggaaaca
      841 acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga attagtacct
      901 gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat cgctattatc
      961 gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat gatccaaccg
     1021 ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc tcttggcgct
     1081 tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat gccgcatggt
     1141 gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt tggttgctat
     1201 tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc tggggaaaaa
     1261 ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga tgctgcaaaa
     1321 ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc aaaacatggt
     1381 tacggggaag catttggaca ttctacagga catgggatcg gtttagagat ccacgaagga
     1441 ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat cactgatgaa
     1501 ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt gttgatcaca
     1561 gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt atagtttcat
     1621 caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga aaatcactcg
     1681 atttaatgat acactataga agaactttta agctgtaaga gataataaag ttgggtgttt
     1741 tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac gtgatcacta
     1801 tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg aataaataaa
     1861 tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag atcgtgatcg
     1921 ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa ggcgtatacg
     1981 gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca gctcacggaa
     2041 aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac tgttatctag
     2101 aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc agccagcaag
     2161 tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta gctgttgttc
     2221 ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag gaagagaatg
     2281 aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt atttccatta
     2341 gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga attagaccac
     2401 cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga tacgctagtt
     2461 ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca tttaaaaaac
     2521 aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat cgcgattttt
     2581 gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc aatcgagttg
     2641 gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt atccaatgta
     2701 ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt tctatgaatc atcataaacc
     2761 tgttataaat cgggaaaatt ctatggtaga atgaaaacac aaaaaatatc tgggagtggt
     2821 tagatggcag aattgattaa cggaaaagaa ttagctgaaa aaatgcaggc agagattgct
     2881 gaaaaaatcc aaaaattgaa agataatggt atccatcccg gattagtcgt tttattggta
     2941 ggggaaaatc cagccagcca aatctatgta agaaataaag aacgtgtagc aaaagaaatc
     3001 ggaatccatt cattggtaga gcgttatcct gagacgattt ctgaagacga actacttgca
     3061 gaaattgaaa aatacaatca agatcctagt tttcatggta tattggtcca attaccattg
     3121 ccaaagcata tagatgaaga aaagatcctg ttagctatcc gccctgaaaa agacgtagac
     3181 ggttttcatc cgatgaattt aggcagactc cttgcgggta aaccggacaa gattccttgt
     3241 actccatacg gcatcatgaa aatgtttgaa gcttattcta tcgatccggc gggtaaacga
     3301 gcagtagtta tcggacgaag caatatcgtc ggtaaaccga tggctcagct actcttgatg
     3361 gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa atctggctga actggcacaa
     3421 gaagcagata ttttagtcgt agcaatagga cgtggacatt ttgtcacaaa agaattcgta
     3481 aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg atgctaacgg taagctgatc
     3541 ggtgatgtga aatttgatga agtggagcct attgcaggct ggatcactcc tgtgccaaag
     3601 ggtgtagggc caatgactat cacgatgctg atgtatcaaa ccgtaaaatc tgccgaagct
     3661 tttggagcag gtgaatagat ggcacaagaa tatttaactg tcactacatt aacgaagtat
     3721 ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg tatatttaac aggcgaaata
     3781 tcgaatttcc gtttacgtgc caatgcccat caatatttca gcttaaaaga tgaccatgca
     3841 aaaatatctg ctattatgtt caaaggagca ttccaaaaat tacgattcca gccaaaagaa
     3901 ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg aagcgggagg ttcttatcaa
     3961 atctatgtag agcacatgga gcctgatgga ataggtgctt tatatcaaga attggaagaa
     4021 cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc ctaaaaaaat gctgccacgt
     4081 ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag cggttatccg cgatatcatc
     4141 acgacggtga aaagaagata tccaattgtc caattggttt tgttcccaac agttgtacaa
     4201 ggaaaccaag cagcggacaa tgttgtcagg aatatccaga gagtagaaga gatgggcaat
     4261 ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg aggatttatg gccgtttaat
     4321 gaagagcggg ttgctcgggc gatttatcaa gctaatacgc cggtgatttc atctgtagga
     4381 catgaaacag ataccacgat cgctgattta gtagcagatg ttcgggctgc cactccaaca
     4441 gcagctgcag aattagctgt tcctgttttg tcagaagagt tgatgcgcat tcaagaacgg
     4501 caagctagat tgcagcaggc gtttgtacgc cagatccagc gcaaacaaga acggttcgag
     4561 cgtgctcgac gttcctatgt atttcggcag cctgaaagat tgtatgaagg tcagaccatc
     4621 aaattagacc agttgaaaca gcgattatat caagctacac agcaaattta tcatgcgaaa
     4681 gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag tggctccgct ttatcgagtt
     4741 aaagcagctc gccaagaaac tgcttatcta gaaaaacgct tagaagagaa aatccaacaa
     4801 tatatgaaac aacagcagca gcgttttgct caagctgttc aatctcttga tttgttgagt
     4861 ccattgaaaa tcatgggaag aggctacagc tatacgacgt taaatgatca agtagtaaaa
     4921 agtgtcaccg agattcatca aggggatttg ctgacgatcc attatcaaga tggaatcgtt
     4981 aaaggacaag tacaagagat agacaaagga gaataaaaat atggaaaatc caacttttga
     5041 agagtcactg caagaattag aaaaaattgt catgcagtta gaacaaggag acgttccatt
     5101 agaatcagct ctagattcgt ttaaacgagg aatggaatta agcaaacatt gccaagatac
     5161 attagctaaa gcagaagaga ccttgacgaa aatgatgaca gaatcaaatg aagaagttga
     5221 ttttgacgga aacgaggaaa gctgagatga gattaactga cttttcagct attcatctac
     5281 ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca tacaacggat agaggactct
     5341 atgatgcgat gagttattca gtgaaagcag gcgggaaaag aatacgtccc ttattacttt
     5401 taacggccgt tgcttccttt gatgagccta ttgatgttcc cgtctatcaa gtagcagctg
     5461 ctttagaaat ggtgcatacg tattccttga tccacgatga tttgcctgcg atggacaatg
     5521 atgatcttcg tagaggaaaa cctaccaatc ataaagtatt cggcgaagca ttagctatat
     5581 tggccggaga tggtttgctt acaggagcat tccagctaat cagtatggca cacttaggta
     5641 attctcctaa gcttctgctg cttcagcaat tagctgtttg tgcgggatca caaggtatgg
     5701 ttgctgggca agctgcagat atcgagggcg aatcgaaaaa gctttcttta gaagaattag
     5761 catttattca tgaaagaaag acagggcatt tgattcgtta tgccttgctg gctggtggca
     5821 tattagcgaa gcaacctgaa gaaatcttat tgctacttca acgattagcc gaacatttag
     5881 gtttagcttt tcagataaga gatgatctac tcgatgtgat tggtacaacg aaaacattag
     5941 ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac ttacccgcgt ttattaggcc
     6001 tagaaaagac acgtgaggcg ttggagattg agctgttatc cgccaacaag atcatagaca
     6061 aattagaaga ggacgttttt tcttttgatg gcgggctgat gcgccagatg atcaagcaat
     6121 ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc gtagatgtat tagcggtcaa
     6181 acaaggatta tttgaaacaa gagaacaagc caaacgttca gtgatggctg gtttgattta
     6241 caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa attccagtga gtagtgaatt
     6301 aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg ggattaaaat tagaaaaagc
     6361 actcaaagca tttgatctat ctgttgaagg aaaaacattg ttagatattg gtgcatccac
     6421 tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa atgagttatg ctttagacgt
     6481 tggctacaat cagctagctt ggaaaataag acaagatcct agagtcgtcg taatggaaag
     6541 agtgaatttt cgttatgcaa aacccgaaga ctttacctta ggaattccag aagttgctgt
     6601 tatcgatgtt tcttttattt cactaaaact gatgctgcct ccattacatg ctattttaaa
     6661 agaagaggga gaagtgattg ctttaatcaa accccaattc gaagcaggca gagaagctgt
     6721 agggaaaaac ggaattgttc gtgaccctca gacccatcaa aaagttttag aagacatttt
     6781 gtcatttgcg gcacaaggtg gatatgatgt gctggaatta agttattctc cgatcacggg
     6841 gggagaagga aatatcgagt tcttggctca cttgaggaaa gtacccgaaa gcggaacaat
     6901 caattcagct attaatatgg cagaagtggt ttcaaatgca cacgaacagt ttgaccataa
     6961 ataaattgta actgtgaaaa tcaagtgaga agtatgcgga ttcttcttta ttataccaag
     7021 gaaataagct atcattgatt aagaagaata tgctaaggag cgaagacgta atgagaaaaa
     7081 aagatcggca tcgtttgatt actcgattgt tgagtgagca agatatccga aagcaggaag
     7141 aatttgttga aatcctgaaa agcaaaggaa tatctgttac tcaagctacg atttccagag
     7201 atataaagga actgaaactg atcaaggtac cggcttcaga tggcggctac cgttatagca
     7261 taccagctga aacaaacgaa gatgtaaata ctaaattgga aaaactatta aaagacgctt
     7321 tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa aacattgccg ggaaatgctt
     7381 cggcagcagc aaatttgatt gataaacgtt acaagaaaga attattttcg attatcaatg
     7441 atgacgataa tgttttgatg attacacgaa ctgaagacca ggcagtttta ttgaagaaag
     7501 acttcctgca ttacctttaa gttgaggagc gaatgtgatg ctacaagaaa tcagtattac
     7561 taactttgcg attattccgg aattgcggct ttcttttcac gaaggaatga ccgcgctgac
     7621 cggagagact ggtgcgggta aatccattat tatcgatgct ttaggtctgt tagcaggagg
     7681 aagaggttca agcgattata tacgtcaagg agcagaaaaa tgtgtgcttg aagggttgtt
     7741 tgaactacca aaacaagaag gattctctga actgatggtt gaattaggga tcgaaacaga
     7801 tgaggacaat ctgattgttc gacgggatat gtccttaact ggaaaaaatg tttgtcgagt
     7861 gaatggtcat attatcacat tagctaatct gagaagaatc ggcagttatt tagtagatat
     7921 ccaaggacaa aacgaacatc aggaactgct ccagcctgaa tcacatcttg ctttattgga
     7981 tcgttttgga gatgtagctt tccaacagaa gaaaaagagt tatcagcaag aatatatctc
     8041 ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa aatgaaaaat cctatgttca
     8101 gagaatagat atgctccatt tccagcaaga agaaatagct gctgctgatt tgcaagtcgg
     8161 agaagaagag aaattaaaag aagaaagaga aaaacttagc aattatcaaa aaattgctga
     8221 tggattagct gcaggttacg gagcactgac gaataatgaa caaaacagtg tagatggtgt
     8281 tggcttggcc gtttcagaac tgcaagggat cgcccatcta gatgtggagt atgaagcaat
     8341 ttatgaaaat atccaaagtg cttactattt gctgcaagat gcaatcggcg atatgagtcg
     8401 tcagattgat ttattggagt tagatgagag tcgtttagaa gaagttacgc aacgtttaga
     8461 attaatccga cagttgaaac gtaaatacgg cgaatccatt gagtcaatat tggcttacta
     8521 tgacgaaatc accgaagagc tagcttcttc tgatttttca gaaggtcaat tggacaaaat
     8581 gaaagaggaa ctagagcaaa aagagcttct acttcagcaa caagccgcag atcttcatga
     8641 agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt ctgcatgaat tgaaaagtct
     8701 gtatatggaa aacactgagt ttgaggtacg tttcttaaaa gaagaaaata gacaattgaa
     8761 tcatgatgga tttgaccaaa tcgaatttta cattacgacc aatccaggag agccactgaa
     8821 gcctttggtg aaagtcgctt ctggtggcga actgtctcgg atgttgcttg ctttgaaaac
     8881 tattttttct tctgagcaag gtgtgacaag tattattttt gatgaagtgg atacaggtgt
     8941 gagcggaaga gttgcacagg ccatcgcaga taaaatttct caaatatcga aatactctca
     9001 agttttgtgc atcactcatc tgcctcaagt tgctgcagtc gccgactatc aatattatat
     9061 tgtgaaagca gtgattgatg gacgtacgca aacttctgtt tcagagttga aaacaaaaga
     9121 aagagaagaa gaaattgcac gtatgctggc tggaagtgag attacaaaac tcacactcga
     9181 acatgcaaaa gaactattga aactcgcaaa aaggacctga gccacctcgc agtggttcag
     9241 gtccttttca gcagctgata gctatatttt atttttacat gacactcatt acagcagtca
     9301 atacaacaga tccaatcgtt aaaatcagca ttgtccaaat gacaaatttt gttactttag
     9361 caaaagtact tgtttgtttt ttttcacgca ttgttttcaa cctcgtcatt aattagttga
     9421 ttcaagtgta caatcttttt tccatttcca caagagggct tttttataaa aacttccctt
     9481 tttttaaata ataggtagtt acgatacaaa taaataatgt taatagaact agccatataa
     9541 aggcatcatc cctttcagca ataggtaact taatgttcat tccatagata ctgccgacga
     9601 ttgtcggtat agttaaaata atcgttaaag aagttaaaat tttcataata ttattcagat
     9661 tattcgatac gattgctgaa aaagtctgat tcatttgttc tactagtttt aattgaatgc
     9721 gagaagtagt cattgcctgc tctgtttcca ccaatacatc acgtaaatga tttttcaaag
     9781 catgattttc ttggaaaagt ttattttgcg ctagttcttt caatgcatct aaattagcag
     9841 aagtcgcttc tctgaaataa acgaggcttt tttggatatc catgatttga taaagttgtt
     9901 tgttttcagt ggatacttga agtttggctt ccaaagtatc gcattgagat tgaagcgtag
     9961 ctaaataact gttgtaagag agtgtgacct gccaaatgaa ttgaaagaag atattgagat
    10021 taggcgaaat atcacttttt ggaaaggtct ggctaaagac tttctttaaa aacagtggtt
    10081 catgattcgt aattgtgata agcttctttt tgggcgtcaa aataatagag agcgggtatg
    10141 tatccacttg aaaataaccg ctaggacttg ttttagcaaa aggatattgt aacaataata
    10201 acgctggctt tgtcaacttt gattgattga attcttctgt acgggaattt tctgcatcgt
    10261 ccaatatgga agtgatgtaa tctgacggaa tttcaaattg atgtgtcagc tgatgaatct
    10321 cttcttgatt aggacgttct acacaatacc agatcgtttg gtcagtttcc tttccggtat
    10381 tacgcagttt ctcattatct aatgtaaaat atttgatcat gattttcctc ctgttgcctg
    10441 tttcttttat tataacggat agggaattgg aatacatgga atgtgtttgt aagatataga
    10501 atataactgc ataaatttaa ataaaatgaa acagttgtgt aaagtatata cagtatactt
    10561 gaagcaacga taaaaaagga gtttacgttc atgggcacat tcacaccact tatcttgttt
    10621 tggtatattt acccggtaat cgttttgttt ggttgccaat ttcttgtaaa attattttcg
    10681 ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt gtggggcggt
    10741 atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt ctttatttct
    10801 gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga agaaattatt
    10861 tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat cgtcttgtac
    10921 gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa aagctgtaaa
    10981 aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg aataaaaaaa
    11041 gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt agactaattt
    11101 tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac ataatattga
    11161 tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt
    11221 tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct
    11281 ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt
    11341 cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag
    11401 cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg
    11461 aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt
    11521 tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta
    11581 atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac
    11641 atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat
    11701 ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca
    11761 atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc
    11821 aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc
    11881 attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt
    11941 tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat
    12001 cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag
    12061 gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc
    12121 caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga
    12181 aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa
    12241 ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga
    12301 atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa
    12361 tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga attccaacca
    12421 gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac
    12481 aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag
    12541 aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga
    12601 tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc
    12661 accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc
    12721 gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc
    12781 tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag
    12841 tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga
    12901 aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa
    12961 taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt
    13021 aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc
    13081 gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt
    13141 gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt
    13201 ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac
    13261 ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct
    13321 gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa
    13381 aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa
    13441 gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca
    13501 tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc
    13561 agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat
    13621 cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc
    13681 aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga
    13741 cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc
    13801 agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca
    13861 acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg
    13921 taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc
    13981 agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca
    14041 agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg
    14101 tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg
    14161 acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga
    14221 tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta
    14281 tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa
    14341 taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga
    14401 agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa
    14461 agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg tttatggtgt
    14521 atatagtgta cctggataca atgtatcggc aaaaaccgga acagcccaaa tcgcttctga
    14581 tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc
    14641 ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg
    14701 aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac
    14761 tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa
    14821 tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat
    14881 cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg
    14941 agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc
    15001 aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga
    15061 tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag
    15121 ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag
    15181 cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg
    15241 gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca
    15301 atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga
    15361 caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt
    15421 ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga
    15481 aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca
    15541 tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag
    15601 atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa
    15661 atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt
    15721 ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct
    15781 taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt
    15841 ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc
    15901 taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg
    15961 tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa
    16021 ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt
    16081 tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa
    16141 atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc
    16201 ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga
    16261 aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata
    16321 acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca
    16381 gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt
    16441 acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg
    16501 aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa
    16561 ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca
    16621 aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat
    16681 ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt
    16741 acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat
    16801 tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca
    16861 cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac
    16921 tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag
    16981 cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat
    17041 gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta
    17101 ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact
    17161 aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac
    17221 agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta
    17281 tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata
    17341 gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt
    17401 gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact
    17461 agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg
    17521 ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct
    17581 tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc
    17641 aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga
    17701 gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct
    17761 taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg
    17821 tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac
    17881 agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt
    17941 ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt
    18001 tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa
    18061 aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc
    18121 acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca
    18181 agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga
    18241 aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag
    18301 tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt
    18361 accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat
    18421 gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc
    18481 actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg
    18541 agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca
    18601 aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg
    18661 tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat
    18721 ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca
    18781 cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag
    18841 tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat
    18901 aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt
    18961 cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat
    19021 gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc
    19081 caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt
    19141 catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat
    19201 atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct
    19261 ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa
    19321 aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa
    19381 atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct
    19441 gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac
    19501 ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa
    19561 gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat
    19621 ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt
    19681 gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa
    19741 acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt
    19801 caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac
    19861 tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt
    19921 gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga
    19981 atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata
    20041 ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta
    20101 ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata
    20161 aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga
    20221 tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa
    20281 tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt
    20341 cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag
    20401 attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat
    20461 tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat
    20521 gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg
    20581 aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg
    20641 gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag
    20701 aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg
    20761 cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag
    20821 aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt
    20881 cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc
    20941 aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc
    21001 ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa
    21061 atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag
    21121 caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag
    21181 ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg
    21241 aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa
    21301 tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa
    21361 ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt
    21421 ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt
    21481 acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta
    21541 ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg
    21601 gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc
    21661 actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg
    21721 aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg
    21781 aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta
    21841 ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa
    21901 gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc
    21961 acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa
    22021 ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg
    22081 aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt
    22141 cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc
    22201 gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa
    22261 atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa
    22321 cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta
    22381 ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg
    22441 gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc
    22501 gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa
    22561 ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc
    22621 tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga
    22681 tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga
    22741 attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa
    22801 gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg
    22861 cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag
    22921 attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa
    22981 tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga
    23041 atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga
    23101 agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt
    23161 tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga
    23221 ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa
    23281 agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt
    23341 tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg
    23401 ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg
    23461 cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc
    23521 agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga
    23581 aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca
    23641 ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg
    23701 gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg
    23761 attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga
    23821 tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag
    23881 atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg cactattact
    23941 tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct
    24001 ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga
    24061 accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat
    24121 tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg
    24181 gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata
    24241 ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt
    24301 tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac
    24361 cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga
    24421 tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt
    24481 ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta
    24541 tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg
    24601 gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat
    24661 tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt
    24721 acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga
    24781 aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc
    24841 cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa
    24901 tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag
    24961 agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg
    25021 tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc
    25081 caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc
    25141 ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa
    25201 gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac
    25261 caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa
    25321 tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa
    25381 aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat
    25441 gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga
    25501 gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg
    25561 gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc
    25621 aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca
    25681 ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg
    25741 cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg
    25801 atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct
    25861 tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca
    25921 ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac
    25981 atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa
    26041 accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc
    26101 gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa
    26161 ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta
    26221 ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc
    26281 tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga acaagtattg
    26341 gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt
    26401 gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca
    26461 ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc
    26521 gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg
    26581 tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct
    26641 ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca
    26701 gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta
    26761 aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa
    26821 gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa
    26881 tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca
    26941 cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct
    27001 ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca
    27061 cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat
    27121 gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta
    27181 gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg
    27241 gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta
    27301 gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac
    27361 cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat
    27421 gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt
    27481 gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga
    27541 tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc
    27601 tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc
    27661 ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc
    27721 acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt
    27781 gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa
    27841 aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat
    27901 gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa
    27961 atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat
    28021 actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa
    28081 gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt
    28141 acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat
    28201 gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat
    28261 caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa
    28321 atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat
    28381 gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt
    28441 gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg
    28501 gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat
    28561 gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat
    28621 gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca
    28681 gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa
    28741 cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta
    28801 gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac
    28861 ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca
    28921 gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc
    28981 agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac
    29041 gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa
    29101 cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt
    29161 tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa
    29221 ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt
    29281 cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa
    29341 tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg
    29401 aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc
    29461 tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc
    29521 accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg
    29581 gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg
    29641 cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag
    29701 tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt
    29761 aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa
    29821 acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat
    29881 atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc
    29941 agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg
    30001 tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac
    30061 acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg
    30121 ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt
    30181 tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg
    30241 ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc
    30301 ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc
    30361 gccgaatatc gtaaacaata catttttttc attagacatg tggatacact cctttatatt
    30421 ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt
    30481 tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata
    30541 ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata
    30601 cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact
    30661 tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt
    30721 tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg
    30781 acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg
    30841 cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg
    30901 gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct
    30961 gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt
    31021 tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg
    31081 gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat
    31141 aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact
    31201 cattctttcg tttataaagg atatatatat acattgggag ggatctattt ttcagataac
    31261 aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata
    31321 agataaaaac catactgatc acgtacgact aaacgatatt catctatttc taccacagat
    31381 gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt
    31441 ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt
    31501 tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca
    31561 agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag
    31621 acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt
    31681 tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag
    31741 gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg
    31801 gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac
    31861 aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg
    31921 acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa
    31981 tatcgatagt caattgccta atcggctggc aattgatggt attttagacg gcacgatcct
    32041 tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc
    32101 caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg
    32161 tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt
    32221 gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt
    32281 tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact
    32341 agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca
    32401 agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt
    32461 tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac
    32521 aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac
    32581 agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga
    32641 tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta
    32701 tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa
    32761 aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca
    32821 tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg
    32881 ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag
    32941 ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt
    33001 ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc
    33061 tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg
    33121 tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc
    33181 aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt
    33241 gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat
    33301 cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc
    33361 caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga
    33421 gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt
    33481 cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat
    33541 tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt
    33601 tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa
    33661 acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg
    33721 gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg
    33781 ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag
    33841 attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa
    33901 ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca
    33961 gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca
    34021 acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa
    34081 aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa
    34141 ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt
    34201 ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc
    34261 gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag
    34321 aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt
    34381 ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat
    34441 gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg
    34501 ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg
    34561 cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa
    34621 actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact
    34681 ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa
    34741 aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct
    34801 atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc
    34861 aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag
    34921 ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg
    34981 cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt
    35041 caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg
    35101 gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac
    35161 cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg
    35221 aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt
    35281 ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga
    35341 tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag
    35401 acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc
    35461 tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg
    35521 aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt
    35581 tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg
    35641 agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta
    35701 tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg
    35761 gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc
    35821 atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat
    35881 tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt
    35941 atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca
    36001 tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga
    36061 acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca
    36121 aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa
    36181 ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc
    36241 gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga
    36301 tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa
    36361 tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg
    36421 gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca
    36481 aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg
    36541 cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag
    36601 ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga
    36661 atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg
    36721 aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag
    36781 cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt
    36841 cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta
    36901 aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc
    36961 gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac
    37021 gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg
    37081 cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg
    37141 atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa
    37201 ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt
    37261 cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag
    37321 tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa
    37381 gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata
    37441 tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg
    37501 atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat
    37561 catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga
    37621 aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa
    37681 aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg
    37741 atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa
    37801 gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc
    37861 gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt
    37921 gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga
    37981 gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt
    38041 ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg
    38101 gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt
    38161 tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa
    38221 cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc
    38281 acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg
    38341 aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa
    38401 aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta
    38461 taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt
    38521 tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac
    38581 actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt
    38641 accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc
    38701 cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc
    38761 cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga
    38821 tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc
    38881 attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc
    38941 tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa
    39001 actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt
    39061 cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc
    39121 accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat
    39181 tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt
    39241 gcaaaaatat attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat
    39301 cgtcacagta tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc
    39361 accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa
    39421 aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc
    39481 ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac
    39541 gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc
    39601 taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc
    39661 aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc
    39721 aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg
    39781 ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat
    39841 cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc
    39901 aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc
    39961 tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca
    40021 gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga
    40081 cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc
    40141 cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc
    40201 tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga
    40261 taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc
    40321 cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag
    40381 gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat
    40441 ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc
    40501 tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa
    40561 tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt
    40621 cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca
    40681 ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga
    40741 gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt
    40801 taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga
    40861 aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa
    40921 gatagatctt tcctgcgaat cctcactaac ttttttgtag gaaggggcag caaaatgaca
    40981 aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat
    41041 gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat
    41101 tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc
    41161 ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct
    41221 tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa
    41281 gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat
    41341 cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat
    41401 gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa
    41461 gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc
    41521 gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat
    41581 tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt
    41641 attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta
    41701 agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat
    41761 caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg
    41821 aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca
    41881 tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta
    41941 acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt
    42001 cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg
    42061 gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg
    42121 ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt
    42181 tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct
    42241 cttttttcgt aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt
    42301 cttggattgc ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg
    42361 cgtatacttt ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt
    42421 ctacctctag tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct
    42481 gattttttta gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga
    42541 aattgttcct gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt
    42601 caattccagt caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt
    42661 tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca
    42721 gtattgattt tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac
    42781 attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct
    42841 tgataaagta aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca
    42901 atccctaatt cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat
    42961 ccatttttta agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat
    43021 aattcttttt gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa
    43081 attttcctat ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat
    43141 gttttattaa tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga
    43201 agttcagcaa atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac
    43261 tgcgcagaaa ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa
    43321 tatttatctt cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga
    43381 tcaaacggaa ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta
    43441 aacttaataa cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat
    43501 cccactctat ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac
    43561 gtatcttcgt attttctata agtgatagga gatactgttc cttctttata aagttgcatc
    43621 caatcctcaa agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc
    43681 aagttatatt ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat
    43741 tttcttatct tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta
    43801 tttccacgtt tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg
    43861 aactataaag aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc
    43921 ggcgcaagga tgtgctattt gtcg
//
LOCUS       DACDVT010000015        43364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDVT010000015 DACDVT010000000
VERSION     DACDVT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.015"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(8..2389)
                     /locus_tag="IWL52_000846"
     CDS             complement(8..2389)
                     /locus_tag="IWL52_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9777430.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="IWL52_000847"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="IWL52_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9777431.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="IWL52_000848"
     CDS             3090..3629
                     /locus_tag="IWL52_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9777432.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="IWL52_000849"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="IWL52_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9777433.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="IWL52_000850"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWL52_000850"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="IWL52_000851"
     CDS             4736..5902
                     /locus_tag="IWL52_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9777434.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="IWL52_000852"
     CDS             5948..7444
                     /locus_tag="IWL52_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9777435.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="IWL52_000853"
     CDS             7525..8208
                     /locus_tag="IWL52_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9777436.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="IWL52_000854"
     CDS             8853..10043
                     /locus_tag="IWL52_000854"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9777437.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="IWL52_000855"
     CDS             10145..11098
                     /locus_tag="IWL52_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9777438.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="IWL52_000856"
     CDS             11167..12516
                     /locus_tag="IWL52_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9777439.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="IWL52_000857"
     CDS             complement(12699..13022)
                     /locus_tag="IWL52_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777440.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14317
                     /locus_tag="IWL52_000858"
     CDS             13151..14317
                     /locus_tag="IWL52_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777441.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKNGFCFMKKLVTHILLARLAKQRMMILEIS"
     gene            14383..15732
                     /locus_tag="IWL52_000859"
     CDS             14383..15732
                     /locus_tag="IWL52_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9777442.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15777..16208)
                     /locus_tag="IWL52_000860"
     CDS             complement(15777..16208)
                     /locus_tag="IWL52_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9777443.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16596..19238
                     /gene="alaS"
                     /locus_tag="IWL52_000861"
     CDS             16596..19238
                     /gene="alaS"
                     /locus_tag="IWL52_000861"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9777444.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKNGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEMAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19438..20034
                     /locus_tag="IWL52_000862"
     CDS             19438..20034
                     /locus_tag="IWL52_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9777445.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20156..21304
                     /locus_tag="IWL52_000863"
     CDS             20156..21304
                     /locus_tag="IWL52_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9777446.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21342..21755)
                     /locus_tag="IWL52_000864"
     CDS             complement(21342..21755)
                     /locus_tag="IWL52_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9777447.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21834..22265
                     /locus_tag="IWL52_000865"
     CDS             21834..22265
                     /locus_tag="IWL52_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9777448.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22364..22597)
                     /locus_tag="IWL52_000866"
     CDS             complement(22364..22597)
                     /locus_tag="IWL52_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9777449.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22780..24450
                     /locus_tag="IWL52_000867"
     CDS             22780..24450
                     /locus_tag="IWL52_000867"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9777450.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24597..24983
                     /locus_tag="IWL52_000868"
     CDS             24597..24983
                     /locus_tag="IWL52_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777451.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24970..25668
                     /locus_tag="IWL52_000869"
     CDS             24970..25668
                     /locus_tag="IWL52_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9777452.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25673..26137
                     /locus_tag="IWL52_000870"
     CDS             25673..26137
                     /locus_tag="IWL52_000870"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9777453.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26134..27045
                     /locus_tag="IWL52_000871"
     CDS             26134..27045
                     /locus_tag="IWL52_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9777454.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27280..27819
                     /gene="pyrR"
                     /locus_tag="IWL52_000872"
     CDS             27280..27819
                     /gene="pyrR"
                     /locus_tag="IWL52_000872"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9777455.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27833..29116
                     /locus_tag="IWL52_000873"
     CDS             27833..29116
                     /locus_tag="IWL52_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9777456.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29129..30055
                     /locus_tag="IWL52_000874"
     CDS             29129..30055
                     /locus_tag="IWL52_000874"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9777457.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30074..31354
                     /locus_tag="IWL52_000875"
     CDS             30074..31354
                     /locus_tag="IWL52_000875"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9777458.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31359..32435
                     /gene="carA"
                     /locus_tag="IWL52_000876"
     CDS             31359..32435
                     /gene="carA"
                     /locus_tag="IWL52_000876"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9777459.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32440..35625
                     /gene="carB"
                     /locus_tag="IWL52_000877"
     CDS             32440..35625
                     /gene="carB"
                     /locus_tag="IWL52_000877"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9777460.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35679..36455
                     /locus_tag="IWL52_000878"
     CDS             35679..36455
                     /locus_tag="IWL52_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9777461.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36452..37384
                     /locus_tag="IWL52_000879"
     CDS             36452..37384
                     /locus_tag="IWL52_000879"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9777462.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37381..38097
                     /gene="pyrF"
                     /locus_tag="IWL52_000880"
     CDS             37381..38097
                     /gene="pyrF"
                     /locus_tag="IWL52_000880"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9777463.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38104..38733
                     /locus_tag="IWL52_000881"
     CDS             38104..38733
                     /locus_tag="IWL52_000881"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9777464.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38721..39035
                     /locus_tag="IWL52_000882"
     CDS             38721..39035
                     /locus_tag="IWL52_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777465.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39073..39714
                     /locus_tag="IWL52_000883"
     CDS             39073..39714
                     /locus_tag="IWL52_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9777466.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39745..40449
                     /locus_tag="IWL52_000884"
     CDS             39745..40449
                     /locus_tag="IWL52_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9777467.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40446..41348
                     /locus_tag="IWL52_000885"
     CDS             40446..41348
                     /locus_tag="IWL52_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9777468.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41506..43212)
                     /locus_tag="IWL52_000886"
     CDS             complement(41506..43212)
                     /locus_tag="IWL52_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9777469.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13879 a         7662 c         9433 g        12390 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaac ggattctgtt ttatgaaaaa gctagtcaca catatactgt tagcacgatt
    14281 ggccaaacaa aggatgatga ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta
    14341 taatgccaaa gtcgtgataa tttaaagaga agaggtgcac gaatgaataa ttttgatata
    14401 ttccgcttca aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa acagcccaca
    14461 gaggtccagg aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct
    14521 caaactggta caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca
    14581 gaaaaagagg aagtacaagt tgttattaca gctccaagta gggaactagc tgcacagatc
    14641 tatgaagcag ctacacaaat cgctaaattt tctacaccgg agatccgtgt ctcgaatttt
    14701 gtcggcggta cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt
    14761 gtagtaggca cgcctggtag aatattagac atgatgaatg aacaagcgct gaagattcac
    14821 accgcatttg catttgttgt cgatgaagcg gatatgacat tggatatggg ttttttaaat
    14881 gaagtagacc aaattgcaag tcgattacca gaaaagctgc agtttttagt tttttctgcc
    14941 actattccag aaaaacttcg tccattctta cgaaaataca tggaaaatcc aatcattgaa
    15001 gaaatcaaac caaaagcagt tatttctgaa gacattgaaa attggctgat ttcaacaaaa
    15061 ggtaaagatg agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc ttatttagca
    15121 atcgtatttg ccaatacaaa acagcatgtg gatgaaattg ccgattactt gaaaaaccaa
    15181 ggcttgaaag tagcaaagat tcatggagac atcaccccaa gagagagaaa acgagtgatg
    15241 cgacaagtac agaatctgga gtatcaatat gttgtagcga ctgatttagc tgctagaggg
    15301 atcgatatcg aaggtgtctc tcatgttatt aatgcagaaa ttccagaaga tctagacttc
    15361 tttatccatc gtgtcggacg tactggacgt aatggcttaa aaggaacggc cattacgtta
    15421 tattcgccaa atgatgagca agccattgat gcgatagaaa aattgggcgt ctcatttgaa
    15481 ccaaaagaaa tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa
    15541 cgagaaaaat caaaagatgt attagatccc acgctgattg gtcttgtgaa gaagaaaaag
    15601 aaaaagatca agcctggtta taagaaaaaa atcgattggg caattgcaga aaaaaataaa
    15661 aaagaacgca aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat
    15721 tcacataaat aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa
    15781 gctagacttt ttttcattcc gatacaaagt acttcatctt caataaaagg ttctgtgcaa
    15841 gtatgatacc cttgtttttc ataaaaatct acagccgtca tttcagctac tacataacta
    15901 gtctgacaac cttctgaaac cgcccgtttt tcagcagctt caagcaactg accgcctaac
    15961 ccttttcctc ggtattcttt tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt
    16021 ttcttttcat aacgcaaagt agctactggt cttttttgat caagaaatag ataataggtt
    16081 tgttgcggga tatcttttcc gtcgaattct agttctgcag gaatcccttg ttctaataca
    16141 aaaacttgat atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt
    16201 tttttcatca tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta
    16261 tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga
    16321 cagtgatcaa tagaatgcgt atactaatta agtacgaaaa ggatatgatt tgttttttaa
    16381 gactcacaga aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct
    16441 ttttctattt tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga
    16501 ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt
    16561 tttttgtatt taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag
    16621 ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt
    16681 ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga
    16741 aaaaatattt tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat
    16801 ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg
    16861 agatgctggg taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat
    16921 gggaattttt gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag
    16981 tttatccaaa agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag
    17041 atcacattat tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg
    17101 atacggaaat tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg
    17161 aaaactatcc aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag
    17221 aattcaacca taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg
    17281 ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag
    17341 acttgtttat gcctattatc cacgccgtag aaaagctaag caaacaagta aaaaatggtg
    17401 aaaacccagt aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat
    17461 ttgctattgg cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt
    17521 tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc
    17581 gtttagtccc tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa
    17641 aagaatttat tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg
    17701 aaggtttgga aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc
    17761 tagcaggaaa agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg
    17821 aagagatggc agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg
    17881 atgcacaacg cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat
    17941 ctgctttatt gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat
    18001 cagatagtca attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag
    18061 gtgaagctca aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag
    18121 cggatcaagg atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc
    18181 aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag
    18241 gacaaaccta tcatctattg atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata
    18301 cagcaaccca tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag
    18361 ctggttcttt agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca
    18421 cttcagatga attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc
    18481 cagttgaaac aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt
    18541 ttggtgaaaa atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac
    18601 tgtgcggagg cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg
    18661 aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg
    18721 aactacttga agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc
    18781 aattgaaaga agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa
    18841 aggaaaatga acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag
    18901 atccagaaac gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga
    18961 atcagctgcg tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt
    19021 tagcaaacga acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg
    19081 aagctggact aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg
    19141 gtggtggacg tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg
    19201 cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa
    19261 acaaaagttc aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc
    19321 ctcatacttt tttcaattgg atggcgcatc taatcaaatg gtcggattcg gatggtcttt
    19381 agttgacgaa agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg
    19441 aatagtagaa ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc
    19501 gtacaagcga tggttccttg gctggggttt attccattag gattcattag tttaacaatt
    19561 atccatatca cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt
    19621 ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt
    19681 gatacattag tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta
    19741 gtagcaggct tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca
    19801 gcctttgcag gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga
    19861 ttattttata caggtgcaac ggcagatgcc tatggtgtag actctgctct tttatttaaa
    19921 accttagcag gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata
    19981 acaccattac tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca
    20041 tcttttaata gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa
    20101 tcgtcctctc catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc
    20161 taagaaacaa gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact
    20221 agcgggtatc ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg
    20281 gcttttttca ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc
    20341 ttcatccata ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca
    20401 tccttatgga cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac
    20461 ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt
    20521 ctctttaaca cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca
    20581 aggcaaaatg tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc
    20641 aacagatagt tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga
    20701 atgggtaact ggatggcgta ttgatggata tataggtttt gctttagcat tatatatcct
    20761 ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac
    20821 gccagcagag attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta
    20881 tcatgattta ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga
    20941 agtagatgaa tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga
    21001 ctttaaaaag tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa
    21061 cgaaaaatac tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg
    21121 gcttaatatt catgatttta gagtaattca tcaaggaaaa acactgcaat ttgatgtagt
    21181 ggtccctgag aaattccagt ttgttgatac agaactagaa agaatcttac gtagtcaaat
    21241 agaagaaaaa atcggagatt accaattgga aattacgttt gatcacaatt atcttttata
    21301 ttaaatgaga aactataaaa agactcctta aagtttttaa tttaaagagt ctttttttct
    21361 tttaattgtt tatggagagc ctgacgtgca agattatctg ctcctttatt tttgttttcg
    21421 ggcaaccatt gcaggatcaa taaagaaaat tcggatagaa gttcttggat tctttgaagg
    21481 tagggaagga agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg
    21541 ctgtctgaaa aacagaaaat cgtttcattc tgtaagtcgt ttttctttag aaattctaaa
    21601 gtttttaaaa agacttcgaa ctctgcttga tggtttgtcc ctatagaaag cgggatagat
    21661 agctgttctt gtttaccttg atagacaatc aagattccac ctccactagg accagggttg
    21721 ccttttgtag aggcatcaat gtatactttt aacataaaaa gagattctcc tttttgttca
    21781 aactaatgtt atggtaagat tatatcatta ctgaatcggt gagaggttag agaatgaaaa
    21841 aaaatattcg atggcagcct gaactggcac atacgatcat ctattggtca ttgaccttga
    21901 tcattctgtt ttttagtctg attctttctt tagaaaacac aggtccttat tggaaaagca
    21961 attttgtttt aggtattttt cttgtttttc tatttctagg tttgcggcgt tcttttattt
    22021 taaaatcaaa ctctgtgatt atacgttatg ctgctttttg gaaagaccga gaaatatctg
    22081 tcggacagat tcgatctttg acgccaacta atactggggt gcaaatgatg ttgcaagatg
    22141 agaaagcccc atctgttttc atgatgcgaa aaaaagatca agtagtcctt tttgatcatt
    22201 ttgaaaaaaa cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa
    22261 gctaatttta aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga aaagagtgag
    22321 acgtacggtt catcccgttt agcgtctcac tctatttgtt tatctattca tgcatactag
    22381 tatcggtagt ctgaacgttg cctttcttga catgggcagc ttgtgttcct cgattccctt
    22441 cgatgatctc aaattcgacc acttgatttt cttccaaagt tttgaatccc tcttcttcaa
    22501 tcgcagtaaa atgtacaaaa atttcttctg tgtcatcata actgataaag ccgtatcctt
    22561 ttttgttgtc aaaccatttt acaattcctg tggtcaaagc actcacgacc tttctaaagt
    22621 ctgatcatag ccttcattat aaagagtatt tataagtacg tcaaataaat taaaaacctg
    22681 taattttatg ttttttttct gaataaaaaa aggtataatt ggcatatctt aacaaagaag
    22741 atattttgtt gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt
    22801 tcacaagaaa cagagctaga accgattgtt gaagtggcta gtaaattaga tcttaaggaa
    22861 gacgatttgg aactgtatgg gaaatataag gcgaaaatca atttttctgg aatcaatcga
    22921 tcaaaggata acgctgaggg gcatttgatc ttagttactt ccatcaatcc aacaccagct
    22981 ggcgaaggaa agtcaacgat cactgtaggg ttaggagacg cgctcaataa aatcaataaa
    23041 aaatctgtta ttgcattgcg agaaccctca cttggtcctg tgatgggaat caaaggtggt
    23101 gctgcaggcg gaggatatgc acaggtattg cctatggaag atatcaactt gcattttact
    23161 ggtgacatgc atgctatcac aacagctaat aatgcattat ctgctctttt agataatcat
    23221 attcatcaag gaaatgaatt gaacatcgat gcccgccgag tgatttggaa acgggtagtc
    23281 gatctcaatg atcgtgaact gcgtaaagta attgttggct tgggtgggcc aatccaaggt
    23341 gttccaagag aagatgggtt tgatatcaca gttgccagcg aaatcatggc tatcttgtgt
    23401 cttgcggcag accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt cggctataca
    23461 tttgaccgtc agcctgtgac tgctggagac ttgaaagcag aaggcgctct ggcattactg
    23521 ctgaaagatg caatcaagcc gaatcttgta caaacaattt acggaacacc agcttttgtc
    23581 cacgggggac cgtttgccaa tatcgctcat ggctgcaata gtattttagc taccaagaca
    23641 gcattgagat tagcagatta cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga
    23701 gagaaatttt tagatatcaa agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt
    23761 gtcgccactg tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga acttaaaaat
    23821 gagaatcttg atgctttgaa agtaggtttt gcaaatttaa agcgccatat acgtaatatg
    23881 gaacaatatc agctacctgt gatcgtcgca atcaatgaat tcgtaacaga tacagacaat
    23941 gaactcacac tgttggagca cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt
    24001 gtatgggcag atggagcaga aggcggcgtt gatttagcaa aagctgtagt acgattgatt
    24061 gatagaaaag aagctaatta caagccatta taccgcctag aagaaacgat acaagaaaag
    24121 acagaaacaa tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag caaaaaagcg
    24181 caaaaacaaa tagaagagtt tacgaagaat ggatgggaca aactgccgat ttgtatggca
    24241 aaaacacagt attcattttc tgatgatcct tcactgctag gtgcaccgga agatttcacc
    24301 atcacgatcc gcgaaatcat cccaaaatta ggggcaggat ttttagtagc cttgactgga
    24361 gatgttatga cgatgcctgg tttgcctaaa aaacctgctg cattgaatat ggacgtgaca
    24421 aatgacggag aagtgttggg attgttttaa gcttgattat aatctaccat cgaaggagct
    24481 gtattttgac tacagcttct ttttttagac gatagggaat taattttgtt tacactagga
    24541 tcattctttt tctttattgt attttgagct atactaaagc ataataggag ggtatgatga
    24601 aaacgacatt tataagaaaa accggttttt acggtatggg tagctcgatt cagctaagaa
    24661 tcaacaatga aaaacgcagt gttctcagtc atgaacaaaa gataacgctt gatctggaag
    24721 tgccctttac gatgcaggtc accttttatt ggctaaaaag ccccgtttat acagtgaaag
    24781 aagcaaagcc tgcttatgtg atcacaatga attacttgat tcttcaaatc tatccgtttt
    24841 tatttctagc aactgggttg ggggccgttt tcctacaaaa tttatttttc agtttttttg
    24901 cagttattgt gttgattgcg ttctttttct atataaaaaa ccgggcatat ttgatcaagg
    24961 aggaaaatta tggcaaactc tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg
    25021 atgagagacg agatgggaaa tgcacgaaat atgggattta ccgaacttgt cagacgccta
    25081 gctcaaagaa aacaattaat gatcagaaaa tacgaagatg atcttttaca gcttgctcag
    25141 ttaaggaatg cgatcgtcca tgatcggatc gcagtcgatt ttgtcattgc agagccaaat
    25201 gattggacag tcaagcgaat ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg
    25261 ctgcctcgat ttgcaaaacg tgtaacaggt tttgaacaag atcttccgtt acttgaactg
    25321 ttaaagatag tagcagaaaa aaggtattca caatttccgt tatacaataa aggaaaattt
    25381 gtagcattga ttactttacg taatatcggg ttttggctag caaaagaaag ccaaaaaggt
    25441 ccggttgatt taacaaataa aagagcgttg gatttgatca tccaaaacgg aaagtacaca
    25501 aattatcatt ttgttccagc taaaacacat atttacgagg ttgaagcgat gtttcgtgag
    25561 caagggactt tggaagccgt acttatcact aaagatggga atcccgatgg taatctgctc
    25621 gggatcgtac gacctcgtga tatttataaa caaatagaaa aggattgatt ttttgttaat
    25681 tatttattgg atattcagcg ctttgctagt aggagtggat cagttggtca aatggtggat
    25741 cactgataat ctagcgcttg gagagacgaa aaatcttata ccaggtattt tgtcactgaa
    25801 tcatattcga aacactggag ccgcatggag tatgctagaa gggaagatgt ggttctttac
    25861 tatagtgaca ctcattgcag tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa
    25921 aggtaatcgc tggttcatga ttggattgag tttgattcta gctggtgcga ttggcaattt
    25981 tatcgaccgt attcgtcttg gctatgtagt agatatgttc cagacagatt ttatgagctt
    26041 tccgattttt aacgtagcag atgtgacact tgtatgtgga gtgatttgta tgttaattta
    26101 tattatttta gatgaaaagg atcaaagaaa aaaatgacag aacttaaaac gacgattcac
    26161 catgaatctg gtaggatcga taaagtattg actaatttat tcaccgatta cagtcgttcg
    26221 caaattcagc tgtggctgaa aaatggagcg gtttctgtca atggacagcc agtcaaagca
    26281 aattacaaag taaaacaagg agacgagatt actgtcttaa tccccgaacc agaaacatta
    26341 gatgtggtcc cagaaaatat cccgttgacg atcgtttacg aagatgaaga tgtagcggtg
    26401 ataaacaaac ctcaaggaat ggtcgttcat ccttcagcag gtcatacgca tggcacaatg
    26461 gtcaatgcgc tgatgtacca tatcaaggat ctttctacga tcaacggcgt tattcgtcca
    26521 ggtattgttc accgaatcga caaagatact tccggactgc taatggttgc caaaaatgac
    26581 caggcacacg aatcattagc tcagcaatta aaagataaga cctctttgcg caaatatgtt
    26641 gctttagtcc atggagagat tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt
    26701 tcgaaggaaa accggaaaat gcaggcgata agagaagatg ggaagtcggc tgtcacacat
    26761 tttactgtat tggaacgttt cgaaggattt actctggttg aattaaaact agaaaccgga
    26821 agaacacacc agatccgtgt gcatatgaag tatatcggtt atccgttagc tggagatcca
    26881 gtatacggac cgaagaaaac tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta
    26941 ggttttacgc acccgactac aaacgaaaag atgatctttg aagcacctct tccagaagtt
    27001 tttgaaaaaa ctttagaaaa attaagaaaa aacattgcat tttaatttag ataacggtat
    27061 agtaattaac aacgaaagca taaaacttaa tagaattcct ttaagtttag tcccgtgagg
    27121 ctaagaaggg tgggagcaag gatacgtatg gtgcgccata agtagattgc tcattgacat
    27181 agtatagcta tcagtgtatc agtatacgct cagtctaaac attcctcctg tcctcttagg
    27241 gcaggagttt ttttatgcaa aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta
    27301 gatcaggtaa ccatgaagcg agcattgact cgaattactt atgagattat cgagagaaac
    27361 catagtatcc aagatatcgt attggtaggt atcaaaacac gaggaatcta cattgcatca
    27421 agaattgccg aacgcctgaa gcagctagaa gatatcgata ttcctgtagg agaactggat
    27481 atcactttat accgagatga caaaaaagaa aatccagaag aaccggaact tcattcttct
    27541 gacatcccag tttccttaga aggaaaagaa gttattttga ttgatgatgt tttgtataca
    27601 ggacgaacga ttcgagcagc aatggacgca gtaatggatt ttggcagacc aagaaaaatt
    27661 tcattagcag tcctggtcga tcgtgggcat cgagaattgc caatccgtgc agattatgtt
    27721 gggaagaaca ttccaacatc tcgggcggaa gagattttag tagagatgca agagcttgat
    27781 ggacaagatc gcatcatgat tttgaaagag gaagattaga ggagagagaa acatgtcgga
    27841 gaaaaaagga tttcataatg atgacgtggt gttagatatc cacgatcgcc caaccccagg
    27901 acattgggta ggattaagtt tacaacactt gttcacgatg ttcggagcaa ccgtgttagt
    27961 ccccatctta gtaggtatcg atccaggtat tgcgctagtc agttcaggac taggtacatt
    28021 ggtgtactta tttactacta aaggaaagat acctgcgtac ttaggaagca gcttcgcttt
    28081 cattgcagcg atgcagcttt tgatgaaaac agatggttat ccagcgattg cacaaggagc
    28141 agtcacaact ggattagtct atttgattgt tgcgctgatc atcaaaaaga tcggttcagc
    28201 atggctggat aaaatcttgc ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt
    28261 gggacttgcg gcaaatgcag caacaaatgc tatgtataat cagggcacgt atgatttcaa
    28321 gtatattgct gtagcgctga tcacattagg attaacaatt ttcttcaaca tgtggctaag
    28381 aggttttctc ggattgattc cgattttact aggtattatt agcggataca tcgtagcctt
    28441 gctgtttgga atcgtggata ctaagccaat cgtagacgct gcctggtttg ctgttcctaa
    28501 ctttgagatt ccctttgtac aatatgaacc aaagctatat cttggagcaa tcacaacaat
    28561 ggcacccatc gcttttgtga cgatgacaga gcatatcggc catcttatgg tattgaataa
    28621 actaactaaa cgtaatttct ttgaagaacc gggtcttcat aagacgttga caggtgatgg
    28681 attagcacaa atcgtggcag cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa
    28741 cattggtgtt ctggctatca cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt
    28801 atttgcagtc attttaggat tcgtaggaaa attaagcgct ttgattttaa gtatccctgg
    28861 accagtcatc tcaggaatca gttttatctt atttggagta attgccgcaa gtggaatgaa
    28921 gattttaatc gagaacaaaa tcgattttga taaaaagaaa aatctgttga ttgcttcagt
    28981 tatcttagta gtaggaattg gcggactcgt ctttgaagta ggcacattta cattgtcggc
    29041 aatggcatta gcaactgtac ttggcatcat tttaaatctt atcttaccag aaacttcacg
    29101 aagcgaagaa aactaaggag gaaacattat gatcatcaaa tcagaacgta tcagcttaaa
    29161 gcatttattg accgttgagg cacttactga tcaagaagtt atgggcttga tccgcagagg
    29221 gcaggaattc aagcaaggtg ctaattggac tccgcaaaag cgccagtact ttgctacaaa
    29281 cctctttttc gaaaatagta ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact
    29341 agggctagaa gtgattgaat ttgaagcaag cacaagctct gtccaaaaag gggaaacact
    29401 atacgataca gtactgacga tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg
    29461 tgatgagaac tattatgacg aactgataca aagtaaaaca atccagtgtt cgattatcaa
    29521 tggaggagac ggaagcggac agcatccaac ccaatgcttg cttgatttga tgaccattta
    29581 tgaagaattt ggtcattttg aaggtctgaa agttgccatc gtaggtgata tcactcactc
    29641 tcgcgtagct aaatccaata tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc
    29701 aggtcctaga gaatggtacg acgatgaatt cgaagtgtac ggacattata tgccattgga
    29761 tgaactacta gatcaagttg atgtcatgat gatgcttcgg gtgcaacacg aacgccatga
    29821 tggaaaagaa agtttctcaa aagaaggcta ccatcaggaa catgggttaa cagttgagcg
    29881 tgcgaagaag ttgcaaaaac atgcaatcat tatgcaccca gcaccagtta accgtgatgt
    29941 agaattagct gattcattag tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa
    30001 tggagtattc gtcagaatgg caatcttaga agcagtgcta aatggaaaag cgtaattata
    30061 ggaggatgaa aaaatgaaaa cactcattaa aaacggtcag atcaatacac gaaaaaatat
    30121 gacgacacca gcagaaattt ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt
    30181 ctcagaagcc gaatttgatg aagtgtttga tgcaaaggga caattgatca cacctggttt
    30241 agtggacgtc catgttcatt tgagagagcc aggatttaca tacaaggaga cgatcgaagc
    30301 tgggactcgc tcagctgctc gtggcggttt cactactgta tgcgcgatgc cgaatttaaa
    30361 tcctgtacct gatacggcag aaaagctgag acaagtatat gacatcatta gaaaagatgc
    30421 agttgtgaag gtattgcagt atgcgccaat cacagaaaat ctgcgaagtg agaaattagt
    30481 tgatcaagaa gcactgattg aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt
    30541 ccaaacagct gggacgatgt atctggctat gaaagaagct gcaaaaaaca ataaagccct
    30601 agtagcccat acagaagatg aatctttatt atttggcggc gttatgcacg ctggcaagaa
    30661 ggcagaagaa cttggtcttc ccgggatctt aagcgtaacc gagtcttctc aaattgctag
    30721 agatctgttg ctagctgaag caactggtgt tcattaccat gtttgccatg tttctactaa
    30781 agaaagtgtc cgtgtaatca gagacgcaaa aaaagcagga atccacgtaa cagcagaagt
    30841 ttcaccccat catcttattt tgatcgacga agatattccc gaagatttcg gcttttggaa
    30901 aatgaacccg ccgctaagag gacgggagga cagagaagct ttgatcgaag gcttgcttga
    30961 cggtacgatc gattgcatcg ctacagacca tgccccccat ggattagaag aaaaaagcca
    31021 aagtttcatg aaatcaccat ttggtatcgt aggaagcgaa acagcgtttc aactgatcta
    31081 tactcatttt gtagaaactg gtcgattcac gttggaacaa gtgatcaatt ggttggcagt
    31141 taaaccggct gagatttttg gtttgaatgc aggaacgcta acggttggtg cccccgctga
    31201 tgtagcagtt tttgatatta cacaaacttg cacgatcgat aaagaagact ttttatcaaa
    31261 aggggagaat acacctttta tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt
    31321 caacggaaaa cttgtgtggc agaagggaga ataaaagtat ggaacgtttg cttatattag
    31381 aagatggaac agttttcgaa ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa
    31441 tcgtttttac aacaagtatg actggttatc aagaaactat cactgatcaa agttttaacg
    31501 gtcaaatcat tacatttacc tatcctatgg tgggtaatta tggggtaaat agagatgatt
    31561 atgaatcgat ttctccaaca tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt
    31621 caaactggcg aaaccaaatg acgctagatt catttttgaa acaaaaagga attcctggaa
    31681 tatcaggtat cgatacgcgc gcccttactc gcaaaatccg tgaagtcgga acgatgaaag
    31741 ctagtatagt tgatgctgga gattccttcg aacatgcatt tgatcagctg aaagcaaccg
    31801 ttctagcaac caatcaagtg cagcaagtct caactaacaa accttatcca agtccaggaa
    31861 caggtcggaa agtcattgtc attgacttcg gcttgaaaca tagtatttta cgagaattga
    31921 gtaaaagaaa ctgtaacctc acagtacttc cctataacac agatgccgag acgattttag
    31981 cgctatcacc agacggagtc atgctgacga atggacctgg tgatccaaaa gatgtgccag
    32041 aagcaatcga aatgatcaaa aaaatccaag gaaaagtacc gatttttggt atttgtctcg
    32101 gtcaccagtt atttgcttta gccaacggtg cagataccta taaaatgaaa ttcggacatc
    32161 gaggattaaa tcacccggta cgagaagtag cgaccggaag aattgatttt acttctcaaa
    32221 atcatggcta tgcagtcgat gaagccacga ttgatccaga aaaactcatc gtaactcatg
    32281 tagaagtaaa tgacggaaca gtagaaggag tcaaacacag agattatccc gctttcagtg
    32341 tgcagtttca tccagatgct gcccctggtc cgcatgatgc actacattta tttgatgaat
    32401 ttatggaaat gatggacgca ggaaaggagc agtaaacaga tgccgaaacg tacagatatc
    32461 aagaaaatta tggtgattgg atctggtccg atcatcattg gtcaagccgc agaatttgac
    32521 tacgcaggaa cacaagcctg ccttgcctta aaagaagaag gatatgaagt ggttttagta
    32581 aattctaatc ctgcgacgat catgacggat aaagaagtgg cagatcaagt atatatcgaa
    32641 ccgatcacac ttgaatttgt atcaaggatt cttcgaaaag agcgtccaga tgctctcctt
    32701 ccaactttag gcggacagac aggcctcaat atggcaatgg agctggcagc atcaggtatt
    32761 ttagaagaat tgaacataga acttctagga acaaagcttt ctgcgatcga tcaagcagaa
    32821 gatcgtgatc tattcaaaca gttgatgaaa gaattgaatc agccaatccc cgagtctgaa
    32881 atcgtcaata cagtagaaga agctgtagat tttgcaaata aaatcggcta tcctgtcatc
    32941 gttcgtccag cctttacttt aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa
    33001 ttacgtatcg tatcagaaaa tggattaagt ctttcccccg ctacccaatg tttgattgaa
    33061 cgaagtattg caggttttaa agaaattgaa tatgaagtga tgagagattc ggctgataat
    33121 gcaatcgttg tctgcaatat ggaaaatttt gacccggtgg gtatccatac aggtgattct
    33181 atcgtatttg cccctagtca aacattgtct gaccatgaat atcaaatgct tcgtgatgct
    33241 tctctttcga ttattcgagc acttaagatc gagggaggct gtaatgtcca actcgctctt
    33301 gatccacata gttttaatta ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct
    33361 gcgttagcaa gtaaggcgac tggctatccg atcgctaagc tagctgctaa aatagcgctt
    33421 ggattaacat tagacgaaat gaaaaatcct gttacaggaa cgacttttgc agaatttgaa
    33481 cctgcattag attatgttgt agctaaaatt cctcgctggc cgtttgataa gtttgaaaac
    33541 ggagaacggc ttttaggcac gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg
    33601 aatatcgaag agtctttatt gaaagctgtg cgttctttag aaatcggtac acaccatatc
    33661 gaacttcctg agttggcaga catcacggac gatgacctaa tccaaaaagt ggttcgagca
    33721 caagatgatc gcctgtttta tgtagctgaa gctattagaa gagggtacac catagaagaa
    33781 ttagcggatt tgacaaaaat cgatttattc tttttagata aactgttgca tatcgtagaa
    33841 atcgaacagc agttgaaaca gcagccgttg gatgacaaga tattgaaaac agcgaaacaa
    33901 aacggtttta cagacaaaaa agtggcggag ctgtgggaaa taaacgaaga agccgttcgc
    33961 cagatgagaa aagaaaatga tttgattcct gtatataaga tggttgacac ttgtgcagcg
    34021 gaattcgaat caaaaacacc ttatttttat agttcctatg aatatgaaaa tgaaagttca
    34081 cgatcagaaa agccctcaat tctagtactg ggatctggtc ctatccgtat cggacaaggg
    34141 gttgaatttg actatgcgac tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa
    34201 gcaatcatca tgaacagcaa tcctgaaaca gtttcgactg acttttctat ttcggataaa
    34261 ttatattttg agccattgac cttcgaagat gtgatgaatg tgatcgagct agaacagccg
    34321 ataggagtaa tcgttcaatt tggtggtcag acagcaatca acttagccga acctttgact
    34381 gctgctggtg taaaaatctt agggacaacg atcgaagact tggatcgtgc ggaaaatcgt
    34441 gatttattcg agcaagcgct gaaagagttg gatattcctc aacctttagg ggatactgct
    34501 acaagtaaag aagaagccgt ggttatcgct tcgagaattg gttatccagt tcttgttcgt
    34561 ccaagctatg tattaggcgg acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa
    34621 gattacatgg aacatgctgt gaaagcatct ccagaacatc cagtactagt ggaccgttac
    34681 ttgcttggtg gtgaatgcga agtggatgcg atttgtgacg gtgaaacagt actcatccca
    34741 gggatcatgg agcatattga gcgtgctggt gtccattcag gggattcaat ggctgtgtat
    34801 ccacctcaaa cattatctga atcgatcaag aaaacgatcg aagattacac gatccgctta
    34861 gcattaggtt tgaattgtgt cgggatgatg aatatccagt ttgtcatcca tgaaaatgag
    34921 gtttatgtga tcgaagtcaa tcctcgagca agtcggactg ttccattctt aagtaaggtg
    34981 acaggaattc caatggcaca aattgcgacg aaagctattt tgggagagaa actaaagaca
    35041 cttggttatg aaaacggact ttatccagaa acaaaagaag ttcatgttaa agcacctgtc
    35101 ttttctttta cgaaattgca gaaagttgat acttacttag ggcctgaaat gaaatcgact
    35161 ggagaagtaa tgggatctga cagacaatta gacaaagctt tgtacaaggc attcgaagct
    35221 tctggattac acttgcctga atatggagcc gtattattta cgattgcgga cgaaacaaaa
    35281 gaagaagcac tagaattagc taaacgtttt acagaaattg gctatagtct gataggaacg
    35341 aaacatacag ctgcttattt cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt
    35401 tctgaggaag cagcagaaaa aaatgtagtc gacttaataa gagatggtaa aacacaagtg
    35461 gttgtcaata cgattgataa agatcgggga aatgcttcta aagacggttt catcatccga
    35521 agagaagcag ttgaacatgg tattccattg tttacttctt tagacacagc agatgcgatc
    35581 gttcgagtga tggaatcccg tgctttttca actaacccta tttaacggcg agcacatgtt
    35641 aagacaaaaa gacgaaaaac cgttgcagga ggggacagat gaaacaagaa atgatggaaa
    35701 tcgttcagca aaaacagctg gcaccaaaga tttttgaaat gacattgaaa ggtgatctag
    35761 taaaagaaat gcagcaacca ggacagttct tgcatattct cgtgccgcgt gcagacttgc
    35821 tattacgccg accgattagc ttgaatcata tcgaccacga aacaaatact tgccgtatca
    35881 tttatcgggt ggaaggtgag gggactagag tcttctctac tctttctgcc ggagaccatt
    35941 tagatgtcat gggtccttta ggcaatggtt ttgatttgtc caatcttcat acaggagacg
    36001 aagtatttat aatcggtgga ggaatcggtg tccctcctct atatgagtta tcaaaacagt
    36061 tagtcgaaaa aggaattaag ccaacgcatt ttttaggttt tgccacacat gaagtcgttt
    36121 attatgaaaa agaatttaat cgtctaggag aaacaagaat cgctactgac gatggaacat
    36181 acggtatgca cggaaatgtc ggtaatttat tgttaggaac gaagacggaa cctacagctg
    36241 tttttgcatg tggttcaaac gggttattga agacagtgga acagctgttt tcttcgcatc
    36301 aaaatgtcca gttatcatta gaatcccgca tggcatgtgg gataggggct tgctatgctt
    36361 gcgtttgtca tacgccggaa gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag
    36421 gaccgatttt taaaatagga gaggtaatta tatgaatgag aatcctttag cggtcaaact
    36481 tcctggccta gaattaaaaa atcctatcat gccggcaagc ggctgtttcg gttttggcaa
    36541 agaatacgga aaatactatg atttgaatca gcttggatcg atcatggtca aggcaacgac
    36601 tccaaatgcc cgctttggca atcccacgcc aagagtggcg gaaacaccat ctggcatgtt
    36661 gaatgcaatt ggtttgcaaa atcctggtct agacgttgtt atgcataaac ttcttccaga
    36721 attagaggag tacaatgagt tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga
    36781 ctatgtagaa gtttgtagca aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa
    36841 catttcttgc ccaaatgtca aacatggcgg aattgctttt ggaacagatt ctgatgttgc
    36901 ttttcaatta acacaagcag taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc
    36961 cccaaatgta acagacatcg ttccaatcgc tcaggcaatc gaagctggag gggcagatgg
    37021 gtttacgatg atcaatactc ttcttggtat gcggattgac cttaagacaa gaaaaccgat
    37081 tttagccaat caaacaggtg gactctccgg accagcaatc aagcctgtcg ctattcgttt
    37141 gattcaccaa gtagcaagta tatccagcct tccaatcatt gggatgggcg gcgtgcagac
    37201 agtggatgat gttcttgaga tgtttatggc tggagcaagt gctgtagcta tagggaccgc
    37261 gaatttcacc gatccgtata tctgtcctaa actaattaaa gagctccctg tgcgaatgag
    37321 cgaactaggc atcgaatcgc tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg
    37381 atgaatcaac tagaccaacg accaattatt gcattagatt tttcaactag gcaagaggta
    37441 gaagactttc ttcgattttt cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa
    37501 ttgttctacc aagaaggacc tgaaatcgta cgttatctaa aagaggctgg tcataatgta
    37561 tttttagatc tgaaacttca cgatattccg aatacggtcg aaaaagcaat gcggggattg
    37621 gcaaagcttg gtgttgatct cacctgtgtt catgccgctg gcggtattcg tatgatggag
    37681 gcggcaatgc gaggattaga agaggggact ccagaaggtg gaaaacgtcc gctacttcta
    37741 gctatcactc agcttacttc tacaagtgaa gaagaaatgc atgccgatca actaatcgaa
    37801 gtttctttag aaaaaagtgt gatccattat gctagctgtg ccaaaaaagc aggattagat
    37861 ggtgtggttt cttctgcttg ggaagtagaa gcgatcaaag aaatggcagg agacgaattt
    37921 gtttgcttaa ctccgggtat aagaccagaa ggaacagtcg caggagatca gacaagagta
    37981 gtcacacctt ctcaggcaaa aaagatcggc tcaactttta tcgttatagg tcgaccaatc
    38041 actcagtcaa ctaatcctta taaagcatat caaacgattc aaacagaatg gagctaacct
    38101 aaaatgaatg ttgaacaaag tatcgcaaaa gacttattag aaattgaagc agtattttta
    38161 aatccaagta atccttttac atgggcaagt ggaatcaaaa gcccaattta ctgtgataac
    38221 cgaatcacga tgagctatcc aaaagtccga aaagagatcg caaaaggatt agcaagcaaa
    38281 atcaaagaag cttaccctga agtacaagtg attgctggaa ctgcaacagc aggaatccct
    38341 catgccgcat gggtagcgga aatactggat ttgccaatgg tctatattcg aagtaaggcg
    38401 aaagatcatg ggaaaggtaa ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt
    38461 gtgatcgagg atttgatttc tacaggcgga agtgtcttgg aagcagctga agcagcaaaa
    38521 agagaaggtg cggatatcct tggtgttgcg gctatcttta cgtatgagtt gccaaaagga
    38581 aaagcaaatt tcgaaaaagc cgaaatccct cttatgacgt tgaccaatta ttctgtattg
    38641 attgaagcag ctttagaaga tcgttatatc gatgagcaag aattaactct gctaaaagaa
    38701 tggaaaaaag atcctgaaaa ttggcagatt taagcgcgct tgatgaagat agagaaatcc
    38761 gatttgtgtc tccagaatca tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa
    38821 gcatcttttc atcaggtatg aaaagaaact tctatcgtga tcagtttttg atttttttaa
    38881 aattcatttt tctaagactg gttagcagaa agaagagaat aatctcttct cttttttcta
    38941 ttatatatag attttctcta ttttttggta agggagctgt ttcaaaaatg gtaaagagcg
    39001 gaaatgttgc gaatatcagt tatttaatga tatgatattc ggaaaaagaa atctcaaagg
    39061 gaggtaactt ttatggggtt gaatgaattt attttaggat tgaacaattt agagacgatc
    39121 acaagggcac ctggattttt taaatataca gagcatacag ttgcagcaca ttcgtatcgt
    39181 gtagcatcta ttgcgcaagt tctaggagac attgaagaag cgaatggaca accgatcgat
    39241 tggaaactgc tctatgaaaa atcattgaat catgactaca cagagcgttt tattggtgat
    39301 atcaaaacgc cggtgaaata cgcaagcaaa gagttaagag ggatgctgca aacagttgaa
    39361 gaaaagatga cggacgaatt catcaaccaa gagattccag ctgagttcca agaagtttat
    39421 cgccgtcggt tgttcgaagg aaaagatgaa accgtagaag gagaactatt agccatttca
    39481 gataaggttg accttcttta tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa
    39541 gtttataagg agatgttcat tgaggcagtg actacgatta aaagctttga acatcggcct
    39601 tctgttcagt atttctttaa agagatattt ccggaactat tgaaccagga attttatgga
    39661 aaagaggcgt ttttagaaac cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga
    39721 tatgagatgc agggggaagt atagttgaaa aagaatatgg atgtcgaatg gggctattct
    39781 ggagacaaag gtccggaaaa ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa
    39841 tttccattgc agtcgcctat tcatttgacc aagggggaag aaaccaaaat agaaggagac
    39901 acgctttcct ttcattatca aaagcaacgg tttactgaca aggaattcaa aaacacgatt
    39961 catttagtac cttttgacca actcagttat gtagagttca agaaagaacg ctattatttg
    40021 acagatattc atttccactt gccttctgaa catatcatca acggaaaaca ggaagatatt
    40081 gaatttcatt ttgttcatat gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac
    40141 cagctaatgg tacaggaagg ttggctgtat aatcaagaaa atggggaaag ttggaactta
    40201 gaaaaacatg agcattgggt aaatccggat gtgttctttc cggagcaaaa aagccatttc
    40261 cattatattg gctcattgac aactccgcct accagcgggc caatccaatg gtttgtaatg
    40321 gatcatgtag gaaaactgaa ccaagagttt cttctcccct tcgatggtca gtatgctcga
    40381 ccgaacaacc gaccaatcca gcccttgaat ggaagagaaa tttattattt cgaggagttt
    40441 gagcaatgaa tattcagcag atgaaatacg tggtcgctgt agcaaacaat ggtagtttca
    40501 gagaagcggc aaagaaaatg tttgtctccc agccaagtct ttcagctggt atcaaagaat
    40561 tagaaaccga gttagggatc acgctgttta ctcgaacgaa tcgaggggca tatttaacag
    40621 aagaagggca agacttctta aatcgtgccg agaaaatatt ggtccagctt gatacattag
    40681 aaactcacta tctaacgagt gaaaagagag acaatttttc gatcgctgcc cagcattatg
    40741 atttcttagg tcctttgacc gcacagctac tcgaggaatt caaagacgag atcaaacaat
    40801 ttcggatcat ggagacgaca acagcgagag tgatcgaaga agtcaaagag cagcacagtg
    40861 agatcggcat tatttatatg aatgagcata atcaagctgg tatcagaaga tatttacaac
    40921 aaggagagct aagtggccgc agcattggaa aattcaaaac gcatattttt ttaaggcaag
    40981 gacatccatt agctgaacac gcagagatca ccaaagaaga attgttatct tatcctcaag
    41041 ttcgttttac acaggatgga aataatttcc cgtattttta tgaagacctt atcgagatcc
    41101 ctgatcaaga aacagtgatt tatacgagtg atcgaggtac tttgatgaat atcgttcttg
    41161 aaactgatgc ttacgcttca ggatctggta ttgtgattgg agggattcgc gagcacttgc
    41221 ggttaatccc tttggcagaa ggcgaactaa atgaatttta tatcatttat tcggcgaaac
    41281 gtccgctcag tgaaatcgct caaagattta tcaaagagct tactcggtta ctggaccagc
    41341 agagctaaga aaagcagatg agaacgctgt ttattgaaca ccgttgatca aaaaagaggg
    41401 gttgtgacag aattttgtca caactcctct ttttgtagca aacaatttat aactaattgc
    41461 tgttgtggga aagtacgcaa atatgattct agaaacactc attgattacc tgttttgttt
    41521 catagctgta attttttctt cttctggtgt cacgataatc gtcttttggt tttcataaat
    41581 aacataacca ggcttggcgc catttggttt tcggatatgc tttacctgta caagatcaac
    41641 cggcacttgg gcagagtatc ggtattttga aaaataggca gctaactcgg cagcttctgt
    41701 aattgtttca tctgagggct tgtcactttt aatgatgacg tgcgaaccgg gtatattttt
    41761 tgcatgaagc caatagtcgg tttttttagc tgtcttcatt gtcagctggt cattttgaag
    41821 attgttttta ccaacaagaa tgagcgttcc gtcagaagaa aagtattgat caggctgaga
    41881 tggtttctta cgtttttgtt tttttaagtt cttctttttc aggtatcctt ctgaagtcaa
    41941 ttcttcttta attgcttcga tatccattgg gccggcaatt tccagttgag ataagacaga
    42001 ttcaagatat tggatttcat ctttggcttc ttggatttgt tcgccaatca gcttaacagc
    42061 gttcttcagc ttttggtatc gatggaaata tttctgtgca ttttgatttg gtgtcaatgc
    42121 cggatctagt gctattttga ttggccgatc ttcttcataa taattaggta aaacaacttc
    42181 ttttgcgccg cgtggtactt gtgtcagaaa agtagtaagt agttcgccat ttctacggta
    42241 attctcagcg ttttcggatt ctttgagtgt ttgttcacgt tttttcaatt tatttttatt
    42301 tcgttttagt tcattttcga tttttcgaat cagttctccg ccttgttgtt ttgcccgatc
    42361 tttctctgct ttttcgtgat aatagctatc taataattga ctgagtgttg gataagctgt
    42421 tacagcagat gcctgttcgg acaatacctg ataagcaatc ggtgtgaaaa aatctttatt
    42481 ttccgtttcg taaaaagtag gaataggctg atggctgatg gctgagaaaa attcgtccca
    42541 tacaaccatc ttttcattag gacgttccag taatcttttg gtcagctctt gagcgatatc
    42601 gaaaccgatt ccctgaaact gacgttggat tgcttttgga tctagttcag tttgggaaag
    42661 ccgctgaaag attttttctt tctcggagct aaatgggttc aagacctctt gttttggcgg
    42721 agcaacatat tctacaccag gaagcaaaga acgataagtg ttttgagagc ttccaatgtg
    42781 tttgatcgca tccagtattt ttccagtttc tcgattaact aagacgatcg tactatgacg
    42841 tcccattaat tcaacgatca atatgatatt ttgtagatca cccaactcgt ttcgtttagc
    42901 aaagtggaaa tggatcactc gatcattttc gatttgttcg atgtcttcta aaatagcgcc
    42961 atccaaatat tttcgaagca tcatcacgaa gttaggcgga ttatcaggat tctgataatc
    43021 gatttgggta atctgtacac gagcataact tgggtgcgca gataataata atcgatggtt
    43081 tttgccgcgt gaacgaatca ccagcacgat ttcattttca taaggctgat ggattttcga
    43141 gatccggcca gatagtaacg tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc
    43201 atcaaaggac atggttcatt cctcatttct tttcataagt atattcatta taacgaaaaa
    43261 aagaagaaaa accaatttgg ccgaatagta aagcaaaagt ctagaaaaat cttggactaa
    43321 ttacataaaa aacagtgttt ctatcgttga aatccaaatt ttta
//
LOCUS       DACDVT010000016        43046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDVT010000016 DACDVT010000000
VERSION     DACDVT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.016"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWL52_000887"
     CDS             212..604
                     /locus_tag="IWL52_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9777470.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWL52_000888"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWL52_000888"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9777471.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="IWL52_000889"
     CDS             3164..4774
                     /locus_tag="IWL52_000889"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9777472.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="IWL52_000890"
     CDS             5066..5935
                     /locus_tag="IWL52_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9777473.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="IWL52_000891"
     CDS             6100..7371
                     /locus_tag="IWL52_000891"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9777474.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /locus_tag="IWL52_000892"
     CDS             7368..8663
                     /locus_tag="IWL52_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9777475.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="IWL52_000893"
     CDS             8762..9025
                     /locus_tag="IWL52_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9777476.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="IWL52_000894"
     CDS             9470..9919
                     /locus_tag="IWL52_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9777477.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="IWL52_000895"
     CDS             9976..11394
                     /locus_tag="IWL52_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9777478.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="IWL52_000896"
     CDS             11394..12254
                     /locus_tag="IWL52_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9777479.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="IWL52_000897"
     CDS             12428..13201
                     /locus_tag="IWL52_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9777480.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="IWL52_000898"
     CDS             13348..13713
                     /locus_tag="IWL52_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9777481.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="IWL52_000899"
     CDS             complement(13861..15069)
                     /locus_tag="IWL52_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9777482.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="IWL52_000900"
     CDS             complement(15088..15366)
                     /locus_tag="IWL52_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777483.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15559..16215
                     /locus_tag="IWL52_000901"
     CDS             15559..16215
                     /locus_tag="IWL52_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9777484.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16657..17454)
                     /locus_tag="IWL52_000902"
     CDS             complement(16657..17454)
                     /locus_tag="IWL52_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9777485.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="IWL52_000903"
     CDS             complement(17451..18635)
                     /locus_tag="IWL52_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9777486.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="IWL52_000904"
     CDS             complement(18660..19907)
                     /locus_tag="IWL52_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9777487.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="IWL52_000905"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="IWL52_000905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9777488.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="IWL52_000906"
     CDS             21887..22201
                     /locus_tag="IWL52_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9777489.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="IWL52_000907"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="IWL52_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9777490.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="IWL52_000908"
     CDS             22886..23530
                     /locus_tag="IWL52_000908"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9777491.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="IWL52_000909"
     CDS             23545..23874
                     /locus_tag="IWL52_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777492.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="IWL52_000910"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="IWL52_000910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9777493.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="IWL52_000911"
     CDS             24862..25686
                     /locus_tag="IWL52_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9777494.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="IWL52_000912"
     CDS             25679..26026
                     /locus_tag="IWL52_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9777495.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="IWL52_000913"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="IWL52_000913"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9777496.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="IWL52_000914"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="IWL52_000914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9777497.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IWL52_000915"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IWL52_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9777498.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IWL52_000916"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IWL52_000916"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9777499.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31683..32450
                     /locus_tag="IWL52_000917"
     CDS             31683..32450
                     /locus_tag="IWL52_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777500.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="IWL52_000918"
     CDS             32440..34482
                     /locus_tag="IWL52_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777501.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="IWL52_000919"
     CDS             34763..34912
                     /locus_tag="IWL52_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9777502.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="IWL52_000920"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="IWL52_000920"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9777503.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="IWL52_000921"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="IWL52_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9777504.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="IWL52_000922"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="IWL52_000922"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9777505.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="IWL52_000923"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="IWL52_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9777506.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="IWL52_000924"
     CDS             39329..40411
                     /locus_tag="IWL52_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9777507.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="IWL52_000925"
     CDS             40411..41214
                     /locus_tag="IWL52_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9777508.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="IWL52_000926"
     CDS             41284..42024
                     /locus_tag="IWL52_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9777509.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="IWL52_000927"
     CDS             42035..43039
                     /locus_tag="IWL52_000927"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9777510.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13839 a         7520 c         9002 g        12685 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aatttcttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgagtgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaa
//
LOCUS       DACDVT010000017        42521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDVT010000017 DACDVT010000000
VERSION     DACDVT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.017"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(39..1328)
                     /locus_tag="IWL52_000928"
     CDS             complement(39..1328)
                     /locus_tag="IWL52_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9777511.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(1355..1657)
                     /locus_tag="IWL52_000929"
     CDS             complement(1355..1657)
                     /locus_tag="IWL52_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9777512.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(1682..3775)
                     /locus_tag="IWL52_000930"
     CDS             complement(1682..3775)
                     /locus_tag="IWL52_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9777513.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(4264..5028)
                     /locus_tag="IWL52_000931"
     CDS             complement(4264..5028)
                     /locus_tag="IWL52_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9777514.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(5139..6008)
                     /gene="mdcB"
                     /locus_tag="IWL52_000932"
     CDS             complement(5139..6008)
                     /gene="mdcB"
                     /locus_tag="IWL52_000932"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9777515.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(6033..7109)
                     /locus_tag="IWL52_000933"
     CDS             complement(6033..7109)
                     /locus_tag="IWL52_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9777516.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(7133..7726)
                     /locus_tag="IWL52_000934"
     CDS             complement(7133..7726)
                     /locus_tag="IWL52_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9777517.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(7730..9406)
                     /gene="mdcD"
                     /locus_tag="IWL52_000935"
     CDS             complement(7730..9406)
                     /gene="mdcD"
                     /locus_tag="IWL52_000935"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9777518.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(9396..9695)
                     /locus_tag="IWL52_000936"
     CDS             complement(9396..9695)
                     /locus_tag="IWL52_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9777519.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(9797..11443)
                     /gene="mdcA"
                     /locus_tag="IWL52_000937"
     CDS             complement(9797..11443)
                     /gene="mdcA"
                     /locus_tag="IWL52_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9777520.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(11450..12361)
                     /locus_tag="IWL52_000938"
     CDS             complement(11450..12361)
                     /locus_tag="IWL52_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9777521.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12481..13338
                     /locus_tag="IWL52_000939"
     CDS             12481..13338
                     /locus_tag="IWL52_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9777522.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(13525..13887)
                     /locus_tag="IWL52_000940"
     CDS             complement(13525..13887)
                     /locus_tag="IWL52_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777523.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            14233..15672
                     /locus_tag="IWL52_000941"
     CDS             14233..15672
                     /locus_tag="IWL52_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9777524.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(15679..18231)
                     /locus_tag="IWL52_000942"
     CDS             complement(15679..18231)
                     /locus_tag="IWL52_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9777525.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(18345..19676)
                     /locus_tag="IWL52_000943"
     CDS             complement(18345..19676)
                     /locus_tag="IWL52_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9777526.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(19642..21120)
                     /locus_tag="IWL52_000944"
     CDS             complement(19642..21120)
                     /locus_tag="IWL52_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9777527.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(21425..23260)
                     /gene="tdc"
                     /locus_tag="IWL52_000945"
     CDS             complement(21425..23260)
                     /gene="tdc"
                     /locus_tag="IWL52_000945"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9777528.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            23730..25157
                     /locus_tag="IWL52_000946"
     CDS             23730..25157
                     /locus_tag="IWL52_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9777529.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(25249..25614)
                     /locus_tag="IWL52_000947"
     CDS             complement(25249..25614)
                     /locus_tag="IWL52_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9777530.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(25611..25976)
                     /locus_tag="IWL52_000948"
     CDS             complement(25611..25976)
                     /locus_tag="IWL52_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9777531.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(26168..26416)
                     /locus_tag="IWL52_000949"
     CDS             complement(26168..26416)
                     /locus_tag="IWL52_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777532.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            26415..26543
                     /locus_tag="IWL52_000950"
     CDS             26415..26543
                     /locus_tag="IWL52_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9777533.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            26600..28294
                     /locus_tag="IWL52_000951"
     CDS             26600..28294
                     /locus_tag="IWL52_000951"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9777534.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            28298..28417
                     /locus_tag="IWL52_000952"
                     /pseudo
     CDS             28298..28417
                     /locus_tag="IWL52_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            29063..30196
                     /locus_tag="IWL52_000953"
     CDS             29063..30196
                     /locus_tag="IWL52_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9777535.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            30227..30547
                     /locus_tag="IWL52_000954"
     CDS             30227..30547
                     /locus_tag="IWL52_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777536.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(30576..31040)
                     /locus_tag="IWL52_000955"
     CDS             complement(30576..31040)
                     /locus_tag="IWL52_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9777537.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(31171..32424)
                     /locus_tag="IWL52_000956"
     CDS             complement(31171..32424)
                     /locus_tag="IWL52_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9777538.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(32453..32770)
                     /locus_tag="IWL52_000957"
     CDS             complement(32453..32770)
                     /locus_tag="IWL52_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9777539.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(32796..33116)
                     /locus_tag="IWL52_000958"
     CDS             complement(32796..33116)
                     /locus_tag="IWL52_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9777540.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(33580..34995)
                     /locus_tag="IWL52_000959"
     CDS             complement(33580..34995)
                     /locus_tag="IWL52_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9777541.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(35012..36328)
                     /locus_tag="IWL52_000960"
     CDS             complement(35012..36328)
                     /locus_tag="IWL52_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9777542.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(36478..37176)
                     /locus_tag="IWL52_000961"
     CDS             complement(36478..37176)
                     /locus_tag="IWL52_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9777543.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(37465..38453)
                     /locus_tag="IWL52_000962"
                     /pseudo
     CDS             complement(37465..38453)
                     /locus_tag="IWL52_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(38533..39366)
                     /gene="msrA"
                     /locus_tag="IWL52_000963"
     CDS             complement(38533..39366)
                     /gene="msrA"
                     /locus_tag="IWL52_000963"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9777544.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(39541..41169)
                     /locus_tag="IWL52_000964"
     CDS             complement(39541..41169)
                     /locus_tag="IWL52_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777545.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(41156..42490)
                     /locus_tag="IWL52_000965"
     CDS             complement(41156..42490)
                     /locus_tag="IWL52_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777546.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12942 a         8771 c         7203 g        13605 t
ORIGIN      
        1 ttcttaaaaa atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg
       61 cttaagatcc aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca
      121 ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag
      181 aaaccatgag caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat
      241 atccctgctg tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag
      301 ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct
      361 aaaatgacag catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca
      421 tcaggaacta atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga
      481 cgtacaccag ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca
      541 aacatgatat agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa
      601 acagtgatga tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga
      661 aattcgaaat gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca
      721 ccaaaaagct tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa
      781 gcaatatcat ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca
      841 atggcgaaac aagctccgat aaaatcaccg accaagatca atacgatccc tcgcagaccg
      901 gcaaaatagc caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa
      961 aaaatgtact tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag
     1021 aacgtcagtg caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat
     1081 tcaatagaag ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa
     1141 gttaaacttc caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc
     1201 tttaacgtgc cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata
     1261 gcaatcaatg caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata
     1321 acgcccatgt ttttaccctc ttttcttttt gtatttagat aactccctta tcttgcagaa
     1381 ctggggtgat tttttcttca atttcatttt ttgaaacaat atttttcaaa tagatcatcg
     1441 ccgttttagg atcaactgcg aacttttcga actgcgaacg aaaattttct gctgtgatga
     1501 tcacatctgg tttcatggaa actgcggaag aaatatcaca atggtccagt ttggcttcta
     1561 ctcctaattt gttcaaaaca tcctcagcag acatctgtgc tgcaaaacta cttcctaaac
     1621 ctgcaccgca tacgaataaa atgttcaatt tgttcatctt tgttctcccc cgtatcattt
     1681 attatttctc aactactgaa aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa
     1741 tcaatcgatc caacttctct tcgttgttga tcaattcaat tagttctttc atgatgttca
     1801 gatgcgagta ggagtctact gctgccaaac aaaaaataat ctttactgga tccatttcag
     1861 gattaccgaa atcgattggt tgctcaatcg tagctacgct gactcctaat ttattgaccc
     1921 catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc aatcacgata tacggtccgt
     1981 aatcattcac tgcacgaatc atcgcttcga catagcgctc ttcgacaatg ttttctttaa
     2041 ccaatggtct agcagctagt tcaatcgatt cctcccaagt agtggctttt tctttgatca
     2101 tgatattgct gtccgtcaaa atatctttta acataggctg gatctccctt ttgttgattt
     2161 ctaaattgtt tgtttcgaat aatttttcta acttgccata gatgattcga tctactcggc
     2221 caccactttc tttcatgaca tctattattt gagtgaataa ctccgtcgat tcatgactgt
     2281 taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa gtcttctata accaagatat
     2341 tcgtctcagt taaaatcgga tcgacaacta aaagcggctt atttcgataa ttcaattgaa
     2401 aggtgctgaa aaccagattg acatccaatt tatctactaa ttcgatttct tttgaactta
     2461 aaactgcagt aatctcgatc tgaggaaact tttctttcag attctctgct aacagatttc
     2521 cagtagccat cccatggtta cagacaacga ctgatttata aacataattc aagtctcgct
     2581 tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa agctatctcg tcctccagta
     2641 tcttgccacc aattacttct tcgatcgtag gaacaaaatg agtgactgcg cggtagattg
     2701 ggaaataatt tgttcggatg ttttctttca atggattcat gacttggata tgattcttga
     2761 ttcttacaat cagtgcagcc atgtgcttat agagattttc gaataaagct tcttccttta
     2821 gatggaaagg tacgtgtgtc tcgttctcta caaattggat caattggatc gttagtaatt
     2881 gagcttgtac ccactcgatt gaattggaga tatctctagc attgaacgtc tcaatagtga
     2941 aacgaagata atttctttct acttgcggga cattgtccaa ttgaaattcc tcgatcatgt
     3001 ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt atttttccaa ttaatggcag
     3061 agataaattc atgtttcctg attcgctgga tccagatcaa tgtaaaaagc aggatttgtt
     3121 ttttataaat atcatcttct tgtttcaaca acttattgga atagatctcc tgtatttttt
     3181 tgatttccac caaaggaaaa taatggagaa agattttttg agccggtgtt aattgtgcat
     3241 cttgattcat aaagaagtcg gtcattccca gattctgatt gataaaatca tagatcatcg
     3301 tacggataga tcgttctgaa cctttataaa ccagtccttg ctttccataa ctgacgatct
     3361 ctatcccata actgaccagt gttgctcgta accgtctcat atcttcatct aatgtacttt
     3421 tagagatttg aaattcttct tcttttttgt ttagatggat cgggattcgg ctgtaagcaa
     3481 cggctaaaac tagatcaaaa atacgttcgc gccgattcaa aaagccgtct ttcacatctt
     3541 ttatttcagt ttctaatcga gcttgctttt ccttttccag cttcaacaaa aaacctttgt
     3601 tgcgaattgt acggatctcc ggaagctttt tactgctcaa ccagatattc gcttcaagaa
     3661 cttcctgacg gatcgtccgc tgactgacat tgaactcact tgctagatgt tttgctttta
     3721 ctggatattg ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa
     3781 tgatctcctt ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct
     3841 gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc
     3901 atctcctcaa cgaaaaagac atacgcctat tattacaaaa attttgccca tctatacaaa
     3961 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt
     4021 gcttcctatt tttcgtttgg ttgttcttat tatcgcagat gcaagctttt aaacaccatt
     4081 tagtggagag gttgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa
     4141 tcgcttccca tattttacaa aatcgtgcct tattgccgat aggctatctc tggcacaccg
     4201 tttattcaga aaaaggagct gcgacaaggc ttttgtcaca actccgttca taaatatttc
     4261 ctgtcaatcc agcataatcg gctgaataaa atcggcttgc catctttttt ctttctcaga
     4321 aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa
     4381 tcgcccatct tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa
     4441 actattttcg tggatctgtc gataatttcc tacttcctga atagttattt ttttagtatg
     4501 atttcttaca ccgtctgctt tgtttttttg cagaactttc tgatgttcgg caaacagctt
     4561 gtctgtataa cagcttctgg ctttttcagg agtttcttct tcccaagcgg attggatctt
     4621 catgaatatc ttttggattt ccagaaactg tgctttcttt tctttagccg ttcctgacat
     4681 gcgggacaat atctccgctt tcagcctttt cttttgtctt ttattcttga cgatagaaaa
     4741 agcagaaacc cctcctactg acaaaacagc taatgcaaga aatcccgttt gctcataact
     4801 agaaggacct gtacctggac cataataccc ataagaatct ctgccgtaag aacgactgcc
     4861 aaaataactg cccccatatg aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct
     4921 agatgtacct ccaccatgac cacccgcgac agcgaatcct tgggtcggca gagcaaacaa
     4981 acataggccc actaacaaaa tgattgctaa ataattgatt tttttcattg tcttcctcgt
     5041 tatcttttat tacaaaaaac tatttttatg atagccttgc taaaaggaag agtcaataac
     5101 cagatgcata aactgttttt ttagcaattt tgtcttattt agtcattgag taacatcgga
     5161 caaatgtatg tgtcggcagt ccataattcc tctatcttca gcatgaacaa gcttgcagac
     5221 aagagatctg cgcttcctcc aggagagatt tggttttcct gacaaaagtg caacagtgct
     5281 gtaaatggac gatttggtaa agcactttga ctagcagcat gagcaagttt ttgcatttcc
     5341 actaacgttt tctgatcact gcgatgcaat acgcatgtgt catctagcga actcatcaaa
     5401 actaacaaca tatgcatttg tttctgtaca tatgaatcat tatctagctc cttagctgct
     5461 tgaattgcaa gcctgatatg cggataccct aatatcgctt cttcatatgc accatttacg
     5521 ccatattttt gcttcaccct atgaccatga gtggtttttt tatgacgata ttttgtatca
     5581 ggaaaagcag caagcttgct gacatcatca agtattttcg ataaagaaaa aggctgttcc
     5641 atactgattt tttgtgcaat gacactgatc aatagcccca ttgcccaaat cgctccttta
     5701 tgggtattga tgccatttgt agcaatcagc attgcctttt ccgcttttcg cccaatctca
     5761 gcaattcttt ctcttaactc ctgatcaata gcatgatgcc atgctatttg agccatttct
     5821 tcaaaaaaag gagttagcgt cttagcagac ttaagaaata aagcatagtc catatcggaa
     5881 tgtgcacccg catccagtgc atccactaat ccaggttttg gacttaatgt tacttctgcg
     5941 atcaatgctt cctctaccaa cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca
     6001 tttatcatga cactcactcc ttcttcctac tattacttgc tgaaaccttt tttgatagct
     6061 acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac aacttgccgt cattagataa
     6121 gctacaagaa tcatgactcc tgaaggtaac tgcagccaac tatctttaaa caataataac
     6181 agtacttgga aaacaaatac attggctaaa tacgtacgat aagcataaac agacaaccat
     6241 ttgaacaatg gcaattttgg cgaatcgttt acaatcattt tggaagcgcc tatgaagaca
     6301 gcaaagatga tgactaaact gtataaactt tggatcgtat tcaagtaagg tgcattcgca
     6361 aagctcaagt ctcctggatg tgcgagcaat gaacggacag aaaagaacgc gataactaag
     6421 gcaacaggta agaaaagata acgtactctg tccaaatatt gatagacttt ttcgtgataa
     6481 acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa accgatctaa taagtaccag
     6541 ctgtcatgat aaggcccagt caatacttgt gtttgataga acagatacca tgcgacatat
     6601 aaaatcgttg aaccaatcaa tactggccat acatattttt tattagcaaa tactttgtaa
     6661 ccgagccaaa caaaaaacgg catgaacaat tggatctgca gcatcataac ggtataccat
     6721 aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt atccgtatac
     6781 ggagtttgtt gttggactgc tggaaatagc caaagataag ccgtcgtcca tagaagatac
     6841 ggaacgacta gttcaaaaaa cttttctttc aaaaattccc aataagacaa atgctgactt
     6901 gtttttacca tattataaac aattgcaaaa ataaacattg gtgctgtgta tttaacgatg
     6961 acgtaaaagg taccaagaaa agctgtattt gttgcgctgt ggctgtttgc caaagcaaag
     7021 ctcaatatcg tttgcatcat aacagccgtt acagctgata ctttctggaa gtcacttacg
     7081 ttgattttga tttttttgtt ttcaaacatc tctttctctc tctttttctt tattaccaaa
     7141 gttctttggt taatttaggt ccttcgactg ttttcaatag aatttttttc tctggctctc
     7201 tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac tacttgggta tcgaattgct
     7261 caaaaaaagc aggatgcgat tgaattacct gaattggcaa ttcaatcggt gaatttgcgt
     7321 acagcagcaa atcaaaatca ctggtttctt ttactgccgg aatcccagtc gataattcaa
     7381 atcctaagct gccgccgatt ccccatttac atcctgataa tatgtttcta gcagcatgaa
     7441 attgctgata aacaggataa tccatgattt tctcttgtct atacgattct tgttcgatga
     7501 tgtcccacgg atgaatgact tgtttgacgg catgttttga gatttctgtt gaccatctct
     7561 gattgcgggc aggtccacgt aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac
     7621 gacgaactgt tccgaacatc ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt
     7681 ctatttcatg tttatcaaaa aggacaatat catgtgcttg aatcattttt tatgcatccc
     7741 attcttcgtc cattttggca cggactttat ttgtttcgac acgtccgaat gttttggctg
     7801 tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg
     7861 cttcattgat tgcttcgatg actttgctga cattttgttc tgaagcagct tggtcggtga
     7921 tatcttctag gaaatgatag atcgctccta gttttgtata attattgata tcgtaagcga
     7981 tagctggaac tttttcagca gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg
     8041 cagcactttc tttagacatc gcttggacgg taatttcttt attgtttaaa gcaatcaagc
     8101 ggcttgattg taatccatgt gctaaaaatg cacctgaaat agcatttcct gcaatcaatc
     8161 caataactgg atgtccagct tgacggagtt ttgcataagc agcagcacta ttagccaaag
     8221 ccacatggat accgatcaat tcttcgttat agccgtatgc ttggcttggt acatcgatca
     8281 caacgatgat tggccgtttc acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa
     8341 tttgtcccat gcggaaacct tcaactaaac caacttcacc gtttcgtaca cggtacatcg
     8401 ggtttttagg atcaggtacg attgatgtca cgatggcttc ttctatgaat ttacttgatt
     8461 taccataata cacagtacta atagaagaag ttggattttt catgccagtt agtgccgcaa
     8521 accaatcgta accaacactt gctgcagctg gttcatttcc ttctgctact tctggtaagt
     8581 caattgattt tgcttgatgg ttagcaattg cttcattgta atcgtcaatg gccattggtt
     8641 ttgaaagatc taaagaatca agtaatgata aataaaatgc tgagctttca ctgcgatgcg
     8701 tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt gattgcttct acactatcag
     8761 tcactaattc atcaatgatt tttgattcta cccgttgacg agatcctaat gtattccaaa
     8821 tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc ttcttgttca ataacttctg
     8881 gtccattcaa accgactctc gctttttttg ttgcgattaa ataactcatt aatgctgaag
     8941 taatcgacat accgccaaat gatcctacac gtcctggaat caaaccgatg actggtacat
     9001 attgttttag agcaatgatt acatttccga tttctgaaat agataacagt ccgtagtttg
     9061 cttcttgcaa acgaacacca cctgtatcta gtacaatgac tggaaagatc tcatttcctt
     9121 ttttattttc ttctaacaca tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac
     9181 cgataccgcc accttggaat tttccttcca tagcgattac tactgcttgt ttgccattga
     9241 tcgtgccacg gctgacaacg atcccgtcat cactttcagg gacgattcct tgtgctggta
     9301 aacttggcgc gatgatttga tcgaatggat caatcaattc acgtccattg tcaagtagag
     9361 cgttcactcg ttctctggcg tttaattcta caaaactatt tttcatctga aagttcctcc
     9421 attgcttgcg tcaatcgtag gtacacaact cctggtgtag caccgaagtc attgatcacg
     9481 atatctgctg aaattggata acgatcaaaa aatctcgtta atacattgtt ccatacttcg
     9541 ccgaagccat cgcttcctgt acaaattttt acagtagatt ctgttttttc tgttggaaac
     9601 atcaagattt ccaaatctcc tgaaccgacc actcctacat ggattttctt attgatcggc
     9661 tgtttcgttg gatatttata agctaattgt tccatggatc tctccccttt ttgtaaaaca
     9721 gagcatacac gttctgctta ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag
     9781 gtttttctcg ccaattttat gaccaagttc tgaatttcat tggtggttca tacaggccat
     9841 ctgaccattc gatcaaatca tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac
     9901 tacgtttgat gcctagatct tctggtaatt tcacgatgcc ggctgcacgc aagctatcta
     9961 gtgattcttt cgtgctagtc attccaagag gtgtaacacc gccaattgct gcaattgctg
    10021 cttgtcgttc ttcttttgtt tttgctttgt acaaataagc aattccttct tctgtcacga
    10081 tatgtgtagt atcttcgcta taaatcatca ctggtgcatt ggctaagcct gcttgttttt
    10141 gaacagctag tgcatctaat tcttcgacaa agactggttt tttatttgaa ccatatgttt
    10201 caaccatttg aacaactaat ttttggcctt tccctaatgg atcatcattg tcgcgaagtg
    10261 attgccatgc tggtgtagaa tgacgacgac cgccaggatt atgtcccatg ttaggcgcac
    10321 cgccaaaacc agataaacgt ccttttgtta cggtagaaga gtttccttcg taatctaatt
    10381 gcaaggttga accgataaac atatcgatgg catattgtcc tgcagcttgt gataatgttc
    10441 ggttactgcg catcgtgccg tctttaccga caaaaaagac atctgggcga gcagcaatat
    10501 atttttccat accaacttcg ccaccaaaac tatggatgct ttcaacccaa cctgattcga
    10561 tggctggaat catcgttgga tgtgggttca atacccagtt cttcgcgatt ttccctttta
    10621 accctagtga ttcaccataa gttggcagta acaattcgat agctgctgta ttgaatccaa
    10681 taccgtgatt caaagattgt acgccgtgtt ctgcgtagat tcctttgatt gccatcattg
    10741 ccattagaat ttgcaattct gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt
    10801 gataaggttt atctgctttc acgacaacgt ctacccagtc accagggata tctacacgtg
    10861 gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat accatctttg aaagctgcag
    10921 cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt gtacaagttg ccgtctttat
    10981 ctgctttatc tgcagctact aaaacaacat ttggaatcaa atcgatgaat aagcgtccat
    11041 aaagttccaa ataagtatgg atatcaccta atttcattgt gccgtcttca atcatttggg
    11101 cgacacgcaa gctttgtgca ccagcataag agaaatcgat cttgcttgcg attccttttt
    11161 caaaaatatt caaatgttct ggtcgtgaga tactagacat gatcaaatgc agatcattca
    11221 ctttttctgc atctactttt tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt
    11281 cgccttctaa tacaactttg tcgccgggag caatcagttt ttccattact tctacgattt
    11341 tttcagtttc tacatacttg ccattgagca agttgctgat tttttccaat cgctgcgctt
    11401 ttttttcgcg tttagaagcc cagttgcggt ttggtttttc cataattagt catctccatt
    11461 tttgatatag aaacgtggcg tcatcaatac catgttggtc tagattgatc gtacggatcc
    11521 ctttatctac accaaatttt gtatgaatca gctttgtcaa agtattgccg gggggaaatt
    11581 caatcgtcag atccattcct aattcttttg ccaagtccat catttggttc cactgcacag
    11641 ggaaagctag gttttccaac aaatctttac gaatctcttc tgcgtcctga gtggctcttc
    11701 catccgtatt ttttatatac aaacatctag catcagatag atggacagac tgcatatacg
    11761 gcttgaattt cttgaccgta tcattcatca agagacagtg agatggcaca gggactttca
    11821 gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc caacgtaacg gataaacctt
    11881 cgatactccc agataacgta tgttgcatcg gacaattttc atttgacaga taaactggat
    11941 ggtcaggatc aaatgttttt gctacaattt cctcagcttc gcttctcgtc acacctacta
    12001 ctacgcccat tgcataaccg cttggataag cttctttcat taagactgcg cggtgataga
    12061 ctagttccgc tgcattctca aatctcaaag atcgacaagc aacagctgca caaaaagcac
    12121 caatcgaatg tcctgctgca atatcaggct gaataccgtt tttttccatt tcatctaaat
    12181 aaaatactgc ttttgtcagt aatgctagct ggataaaaac cgtatcttga tagttttctt
    12241 ctttatcaac taaccgatac cccgtcacat tttctaaacg ttccatgtac atttcaggga
    12301 tatccgctaa catccctaac ttctgactgc cttgtccagg aaataagaaa cctgtactca
    12361 tgagcctcgc ctcctcagca cttacttttt ccactgctat cttagtcaat ttctgctata
    12421 ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg tatggatggg atggataatt
    12481 atgaaattgc ttcatctgca gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa
    12541 gcagccaaag aattatttat ttctcaacct gccttgagcc gtgcgatcaa acatttggaa
    12601 aacgaattag gtgtcccttt gttttaccat agcggcagaa acattgagac gaccgtttat
    12661 gcagaagaat tttatccata tgcagtcaag acattagaca cattagaaga aggaatcaaa
    12721 ttattgtctt cggtcaatga gaagatcgtt acttccgtca tattgtattt agaggtagcc
    12781 tctgtttcta ttccgaactt ggtccgaatt tttatggaaa agcatcctga tatccaatta
    12841 acgatcatgc aacatcattt accagatgat attaccgatc ctgtactgta catcacttcg
    12901 gagatgaagc ctggcatgac caatattcct atcatgaaag aacctgttta tgtcgccatt
    12961 cctaagaaac atcctcttgc taaaaaagaa catctgcaat tagaagatat ccaacgcacc
    13021 cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata cactggatgc agcacttgaa
    13081 gcgaaaagta tcgaattgac gatcagttcg acgacagatg atcctgcgac attacgttct
    13141 attgtcaagc aaggattagc tgttagcttt ttcccaaaag tttcttggtc ttacgataaa
    13201 aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct tgacacgaac aatttattta
    13261 tcttctagct tcacagaaga tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa
    13321 ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt
    13381 tcgaacaaac ggtgttccag aagtaatttc ttagaaaata agccgaaatt tcccaaaaat
    13441 ttgagaagca attttcgtaa attccttctt atttcttgaa gctaaacatt tctgtcacaa
    13501 cctcttctct attgtcctag acattcattg cgttgattta aaacttccat caatcaaatc
    13561 tgctagttca tctagctttt ctattttacg tagatcaata gagatccaat gttctttatt
    13621 catgtgatag gctggaaaat acccctcttt tcctcgcaaa attccgccaa gttcaggatc
    13681 gaccttcaaa tcaagaatat tgatgatcct atggtcactt gcatcatcat agattttttc
    13741 ttctggaaca gacatcagga ccccaaacca ttttttgctt ttttgatgtc tgaatacgca
    13801 ataatcagga aattttttaa aaatatattc tggagacaaa ttgtatgttt cattgatgtg
    13861 atcgattagt tcttttttcg ttagcatctg cttccatctc tttcttattg attcgcttta
    13921 ttcaaagata ccacgtaaga atagaaaaat gaatacaagt agttgctaat caaatacttc
    13981 gatatttttc ttttttatca actcttaaat gttcaatccg cctgtgttgg tcttgctacc
    14041 atctactatt aaaatgttta ttattgtttg cccacctatt ttttgatttt agtggtatca
    14101 tttttcaagt tcatgatgaa attcttatca tcagtaaatg ctccagaaaa ataaacaatg
    14161 attgaaaaat cagttgaaac ttgaccaccg tttattttct ccgtattctt agaatagaag
    14221 ggagtctgat tgatgtattc actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt
    14281 catgtttctt tagtagaaca attgctgaat catacaaaga taaccgtaaa tgaattagct
    14341 gaaatcatag ggacaaccga acgaacgatt ttttctgacc ttcaatctat tcgctctcag
    14401 ctgcccgaag gctgggacat catcagcgat caagcaggaa tcagtttgca aaaccagcaa
    14461 aatttattaa caaatgacct ttgggagatt tttttcaaac aatcagtcag tgtcgaactg
    14521 ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg attttctagc cgaccatggg
    14581 ctttcttatg ggacattgaa aagacatgtg actaaaatca atcagcgttt agcaagttac
    14641 gacttgcaga tcgatttgac gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt
    14701 cgtacttttt atcatcgatt gctgattcct ttcactcata acaactattt tttcgaggat
    14761 tattcgatcc atgaatccca ttattttcaa tttttaagaa acctcagtca aactgaacta
    14821 gcggttgaaa cagaagagat tttcggaaca tgttggttct ttatcaatac gatccgtata
    14881 aaagcgaatt gccgattaga ttcttctatc cataccaaca gcacactctc ctccctgtat
    14941 gactctgcat tgaaaaaatt gtatttaacg gaaggtattt atcttaaaga cactgagctt
    15001 tcttttgctt ctttctgctt tctggaaagt tggaattaca acaataacta tggacaagaa
    15061 accgctcgct gcctgcatca ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa
    15121 ctagcttcag agctgtctct tgatcaacta aaaaaaacat ctctgactga taacttggct
    15181 ctcctcatat taaaatatca tgaatcgccg atattgattg aagcattgga ccgtcaatac
    15241 catcgttttt tagaaacata tgatcagcaa tatgcacact tgtatcaaca gaaagagaat
    15301 ttactgaacg atctaaaaaa agaaatagaa atcaatgaac caaattactt tctccaactc
    15361 ttgtctctac tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat
    15421 tttattttcc aaggagaacc ttcatggaag gcgttcttgc agcaggaatt aaaagattac
    15481 ttaggaaaac gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttt
    15541 gaaaaaacag atatcttgat ttccaatttc cctctagatt ctctagatat ccctattgta
    15601 tacatttctt ctattccaac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa
    15661 acttttttat agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa
    15721 cagtttgttc atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc
    15781 tccaatcaat aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac
    15841 tggtagaaaa ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc
    15901 tttgttttgc aaaagacctt ggttcaatga cggcttggac aactctctgc aattgatcat
    15961 gtaaacagct tgagctaaga cgatattttg cagaagaatg gtttgctgta agctttgccc
    16021 ttcaaactgc atagccaata aataggcagg aaccatgatc agtagggaga catagatgat
    16081 ccgaaacaaa tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat
    16141 gatttctgga tctggcgact cgaaccctag tgcataagat aaagtgattg tcgttaccat
    16201 attgacccat aaaatctgta ctggggacaa aggtagcggg cgattcagca taagtgccca
    16261 aatcaagatc aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa
    16321 attatccaaa atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc
    16381 tcctaaaacc atatcagctg cctgtttgct tacttcacta cctttgattc ccatagcaat
    16441 tcctacatcg gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccgac
    16501 gatttctcca tttttctgca aagctttaac aattcgcagt ttatgttctg gtgtcgttcg
    16561 agcaaagaca tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa
    16621 atcgatccct tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt
    16681 tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttggcattc
    16741 ctttacagct ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa
    16801 agttaattgg gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc
    16861 aaatcctaaa acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc
    16921 ttgttctgca gaagaaagag ttgatttagc aaataatact tctggtgcac ctttgacgta
    16981 aatcaccgct cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa
    17041 cggaatcttt gcttctattt ttttcaaagg tagattggct gtttctgtgt gtcgaatcag
    17101 cgcaacttct gtcggattgc cacttaaatt ttctgttttc tgttcttctt ttgtttttaa
    17161 ttcctgacag tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac
    17221 aactgtcatt tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga
    17281 acctaatgtt tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg
    17341 tactccaacg gatagaatca tggtcaaaac agctggcaat ccttcgggta ccattgacac
    17401 caccaaagca atcgtagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt
    17461 aaaaataact aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg
    17521 gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat
    17581 tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg
    17641 tacaagcgtt cctgaaaaac ccatattttt ctggtcgccg atcgattctt cttctgtcaa
    17701 aacggcatga cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa
    17761 taaatcatgt gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac
    17821 aatgacatct ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag
    17881 tactatcgct tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct
    17941 ttcttgccag taactaacga gtccattgac gatcactacg aataaaataa tacttccttc
    18001 aatatattca ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcatcagcag
    18061 atcagtaaaa tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc
    18121 attatatcct tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga
    18181 tgtttgatag tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc
    18241 tcctgtttaa ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt
    18301 tcaggggggt ctgaaataat tcgttttgca aaatgtatct gggcctaact gatatttcta
    18361 ttgcttattc ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga
    18421 ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac
    18481 aaagcaagat ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca
    18541 tgtgcacaca agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa
    18601 agctgaaaaa acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat
    18661 aacattcctg taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac
    18721 caatggggca aacttttttc ttttgctgtt tgatataaaa tcctgctagt actgtagact
    18781 gcactatttg ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg
    18841 ccttggcaat ggatcacttg cagcatagtc actaaaggac tttcttttaa atctaaacaa
    18901 tgccatggaa caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc
    18961 caaggcagac agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg
    19021 gttaagccga tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc
    19081 attccagcaa agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg
    19141 atagactggg tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa
    19201 atccccaaga tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca
    19261 acaaggttga ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca
    19321 tgtacaaacc agaaatggat atacaatcct attgctgtta attccgcaag tccagctcca
    19381 agccagcaaa gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag
    19441 tcaacgaaat cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc
    19501 cgcatcaaac cataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct
    19561 gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat
    19621 gcgattaact ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag
    19681 ttcacctttt tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat
    19741 tttttcaata cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag
    19801 attgatccat gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa
    19861 aacaataacg acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa
    19921 tggtcgattc aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac
    19981 atagacgata cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac
    20041 gccaggaatc agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat
    20101 aacggatttg gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc
    20161 accttttcta gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg
    20221 gactgctaca ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc
    20281 aagaatttgg caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt
    20341 tggtacgaca tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa
    20401 cgcggcaaat actccgcgaa tatacgtttt aactggattt tccaatcgag tgatatacac
    20461 agaggacatt tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga
    20521 tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg
    20581 tacgatccca actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac
    20641 gtacaatcct aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt
    20701 caaaaaagtg atgatccaat aaacgactaa aatgacaact aatgtaaatt tgctgttcaa
    20761 accaagcggc acattatcga taatattacc aaacaacgga gcgagtgctg aagcgaccat
    20821 caccatccca ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt
    20881 tcctagagca ttcgataccc aaagttctgg accacctttt tggggaaaca ttccagcaaa
    20941 ttcaccagag atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa
    21001 aatcatcgtc cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc
    21061 gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat
    21121 ctttattcct tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa
    21181 cagaaatgac aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg
    21241 aacgatctta gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt
    21301 tataaaaaag aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt
    21361 gatgaagcaa ataattgtct gttctccata tgtctcttgt tattctttca cacaaaaatc
    21421 agttctattg gatgatttcg tttagtttct tttccattgc tttggcgatt ttttttgtat
    21481 aaaaattgaa aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag
    21541 tgattttctg ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa
    21601 tcggagaatc accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac
    21661 cataaacatc gccatccata taagaagacc gatcgaacat tttttcattt aattcattga
    21721 tagcgttcaa atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag
    21781 gatgattcaa tggatagact tcgattgttt ttcctttaac agtaaaagtc agatgatcga
    21841 gaaattctct gaatctttga gctgcctcga ttgaagcccc aatcaattga ccatatccgg
    21901 tcacattcaa tggcaataca cgatgtgctg tccagactgc ggcggcggtt gctccagctt
    21961 ttgatccttc caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca
    22021 catatggcgc gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc
    22081 cagcagcata agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg
    22141 aaatagctct gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat
    22201 gatgttcttc aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta
    22261 gaaacagtga acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatcc
    22321 cttcatcttt taacctttct cttaattgga cgattttatc tacactgtct acttgacctt
    22381 cttctgtcgt tcctacgaca gcaaccacac ctagaattgg gattttttgt ccagctaatt
    22441 cacgaattgt tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag
    22501 caaccatctg atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt
    22561 ttgtttgagg gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag
    22621 ctgctttgac ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt
    22681 caacagataa attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt
    22741 ccttgacagc taaaggaatc gatttgatgc aacgggcgta ccataatcct tcaagattag
    22801 caatagaacc atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa
    22861 aatcctgtcc tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat
    22921 tatttgggtt ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag
    22981 cattcatttg tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc
    23041 gctgactgac ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag
    23101 tgagcttatc tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc
    23161 caagatgttc atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt
    23221 tgtcgcctat aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt
    23281 gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca
    23341 aaaatgagaa tttttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa
    23401 ataaagtcgg atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca
    23461 gtcttctaaa atagataacc taaaaaaagc gaacaaacaa ataaaatgac tgtccttcat
    23521 tcactaaaca tatttttatt tttttatatt gttaaaaatt cgcaatttat tttttaatag
    23581 aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca tttatatgct
    23641 ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt
    23701 gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt
    23761 taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc
    23821 tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa
    23881 aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt
    23941 tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt
    24001 taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat
    24061 gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta
    24121 atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc
    24181 tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat
    24241 cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt
    24301 atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc
    24361 aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt
    24421 acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag
    24481 aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt
    24541 tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa
    24601 aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt
    24661 tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc
    24721 cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa
    24781 ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc
    24841 gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct
    24901 atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa
    24961 ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt
    25021 atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg
    25081 tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag
    25141 ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa
    25201 gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg
    25261 gacatctacg ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac
    25321 caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc
    25381 agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc
    25441 attgttctcg ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa
    25501 atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc
    25561 cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc
    25621 tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact
    25681 gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact
    25741 gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc
    25801 agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac
    25861 caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa
    25921 atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac
    25981 gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt
    26041 cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc
    26101 ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa
    26161 ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac
    26221 ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat
    26281 ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc
    26341 gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca
    26401 aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg
    26461 cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat
    26521 atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga
    26581 agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc
    26641 caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac
    26701 cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga
    26761 tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg
    26821 tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc
    26881 tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg
    26941 aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact
    27001 gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga
    27061 agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt
    27121 tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc
    27181 tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct
    27241 agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga
    27301 gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt
    27361 caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact
    27421 gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg
    27481 gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga
    27541 ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa
    27601 tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac
    27661 gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc
    27721 cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat
    27781 gtctatcgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat
    27841 ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa
    27901 aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg
    27961 accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc
    28021 tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa
    28081 agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta
    28141 tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt
    28201 tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac
    28261 tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct
    28321 gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga
    28381 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttgtg aaaaagctgc
    28441 cctgcatcaa gtaataagaa cacccaagct aaaactgctt cttatgtttt tgacttggtt
    28501 ggacttatta ccgcagatgc aagcttttga acaccattta ccaagaggtt atgaatgccc
    28561 tgcatcaagt aataagagca gccaagctaa aacagcttct tatgtttttg acttggttag
    28621 acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaaggcg
    28681 tttagtttcg agtatgaaga aaaattttgt gaaaatggtt cctcacgttt ttgcataaat
    28741 ttgacttaat accgagaaac tgatcccttc tgtcataacc tctttttatt tatgtctgtt
    28801 tctttttgaa aacaactatt ttttcaacgt ttactctctt gccaatccaa catcatctaa
    28861 cggatacaac tatttcaatg attaccatta cactaaaaaa tcggaaacac acctctccga
    28921 acacaaatac ataagactat agaatgtttc atttaagaaa aaaagcgttt cattatcaaa
    28981 aaggtaagat ctcctttaaa atgaaattat ctgtagtact aataatgcag acaattttat
    29041 gaatctgaaa ggagcatctc caatgaaagc agtcacttgg caaggaaaac agaaaatgga
    29101 aatcagaaac gtagatgatc caaaaatatt agaaccaaca gatgcaatca tccgtatcac
    29161 agcaacagcc atttgcggct ccgatctgca tctttaccat catggtgaag cagttttgga
    29221 aaaagggtat gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt
    29281 tcaaacattg aaaaaagggc aacgtgtggt tatccctttt aatattagtt gtggtcattg
    29341 tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag atcagttata
    29401 tggcgggctt ttcggatttg gcaagttgaa cggtaatcat tggggtggtc aagcggaata
    29461 tctaagagtt ccttatgcag actctacttc atttgttgta ccagataatg atcttcctga
    29521 tgaaaaagtt ttatttctat cagatatttt gcccacagct tattggagcg ttgaaaacgc
    29581 aggagtaaaa aaaggcgata cagttgtgat tctaggttct ggtccagtcg gtctattcgc
    29641 tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc cagtacagca
    29701 ccgtctagat aaagcggcta attacaatgg ggttgaaact tatttcttag aagacacggc
    29761 acaagctgga acagaattgt atgaactggc taaaggtggc gcagatgtca ttatcgattg
    29821 tgtaggaatg gacggattag agcctgtcaa agaaaaagcg aaaaatttag taagcttaca
    29881 aagcggcaca atctctccat tacagatggc atcgcaagct gtcaaaaaat ttggaacagt
    29941 ccaagtaact ggtgtttata tgactccagc ttcttcttac ccgctgcaag aattttttat
    30001 gcgggatatc gctgtcaagc atggacaggc accaattatc catctaatgc caaaaattta
    30061 cgcaatgatt gcagaaggac tattcgatcc tacacaaatc attactcatt cgctgccact
    30121 atcagaagca gcacaagcat atgagatctt tgacaaaaaa gaagataaaa atataaaagt
    30181 tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa
    30241 acgaaatacc catgagatcg aaataggaaa agaagaagat atcgaaatac gaggacgtcg
    30301 cttccgcttc tatttccaaa atcgttacac attgatctca ctggctgtcg atttattaac
    30361 cgggattttc tatatttttg gaagccttgc ttccttaact aaaatccccg atcgatatgg
    30421 cacctatttt tatttggctg gtgctatttt cttgacgatt cgtcctattc tcagaatcgt
    30481 aaggaacatt tttatttacg acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga
    30541 ggcatgagaa tttttcaaat ctcatgcctc ttttttcacc ctatcgtatc actttgatac
    30601 agacgagcgg tcgtcccact ttcttccttg accattgtca aaaatgtcca ctcgtatcgt
    30661 tcataaaacg tggtatgatc tgtaattaag taaagttttt cgtacccaag ttgatgcatt
    30721 tcttttctga tttccgctaa tatccgcttg gctatcccct gcttacgctt ttccggttca
    30781 acaaacaggg cacaaagatt tggtgtcaga tcagggcggt tatggaagtc gttttcaatg
    30841 acgcccgctc ctccgacgat ggtttctttt tcatctaaga ccacatacca ttgaggaata
    30901 ctttccttat tagcgattcc agtacgaata ctttcctcat acacaacagc aggaactccc
    30961 cattttgatg aaaaccaatt cgcgctttgt tttactaatt ctgggtgttc tcttaatttg
    31021 atccagttat atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt
    31081 atacaagtct ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa aaaacggaaa
    31141 acaatcactt gttctccgtt ttttgattga tcaagcatct aaagcttctt ttcttctttc
    31201 ttggatcgta gctaccttga cgaacggtag atagatcaaa atagaaagaa ccaaattgac
    31261 agcggctagt acaccacctg caatactttt tgttgccaat acaccgccaa taattggtgg
    31321 agttacccat ggaggcataa cggtagctgc tggcactaaa cctgtagaag tcgcaaagta
    31381 agcaacacta actaagacca ttggtgtcaa aataaatgga atgaacatga tcgggttcaa
    31441 cacgataggt aacccaaaca tcgttggttc attaatattg aagacacctg gcgcaatcga
    31501 caagtttgtt acaaccatat atggtttgtt tttacgtcct actaaataaa tagctaacag
    31561 taaacctaaa gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt
    31621 cacgataaac ggtgcgctat gcccagctgc gattgcatta acgtttgctt caatggctgg
    31681 cgcattgatt gtttgcatca aaggatcgac catattcgct ccgtgtaaac caaagaacca
    31741 taagaatggt gtgataaatg caagcagtaa ggcagatgga taagagtttg caagtcccat
    31801 aaatggttct tggaccactg tatagaaaga acctacgata tcatcaacac cgaatgcagc
    31861 aaaaattgca gcaactaaag caaacgcaga gatagtgatc attgctggta gtaatgaagc
    31921 aaatgctttt gcaactgccg gtggcacacc atcaggcatt ttgatgatca atttttcatt
    31981 tccgcttaaa cgtacaaaga tttctgttga aataatccca acaataatag caataaacaa
    32041 accgtttgag ctcataccta atgctccgcc taaagcgaag aaagagccta aagaaatcac
    32101 acctgcggaa acaccgtctt ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc
    32161 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    32221 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    32281 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    32341 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    32401 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    32461 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    32521 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    32581 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    32641 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    32701 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    32761 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    32821 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    32881 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    32941 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    33001 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    33061 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    33121 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    33181 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    33241 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    33301 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    33361 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    33421 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    33481 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    33541 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    33601 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    33661 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    33721 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    33781 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    33841 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    33901 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    33961 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    34021 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    34081 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    34141 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    34201 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    34261 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    34321 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    34381 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    34441 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    34501 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    34561 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    34621 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    34681 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    34741 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    34801 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    34861 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    34921 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    34981 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    35041 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    35101 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    35161 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    35221 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    35281 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    35341 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    35401 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    35461 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    35521 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    35581 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    35641 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    35701 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    35761 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    35821 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    35881 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    35941 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    36001 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    36061 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    36121 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    36181 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    36241 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    36301 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    36361 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    36421 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    36481 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    36541 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    36601 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    36661 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    36721 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    36781 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    36841 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    36901 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    36961 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    37021 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    37081 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    37141 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    37201 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    37261 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    37321 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    37381 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    37441 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    37501 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    37561 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    37621 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    37681 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    37741 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    37801 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    37861 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    37921 agtcattttt ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct
    37981 tgtcttacaa gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg
    38041 cgatgatgat agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa
    38101 cttgaaatga tggatttacg agagccccat tccccatatg gcccttccca catatgataa
    38161 cctgctttgc tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata
    38221 cgcccaaagt ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa
    38281 tgggtaattc ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca
    38341 taatcaccaa aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga
    38401 ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct
    38461 cctcctcatc aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt
    38521 aaagaaaagg agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac
    38581 tcgttgatag atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg
    38641 ttttttgtaa aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat
    38701 ttttgtgacg attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc
    38761 cgctaattct ttttgtcgtt cgccttgata aaaaatcact ggacgatatt gcttccctct
    38821 atcttgaaat tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa
    38881 ctcgctataa cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt
    38941 gtaaccacca cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga
    39001 caaaacagat aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc
    39061 ccctgcaaat actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc
    39121 atatttttgc tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg
    39181 cgtcagattc attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc
    39241 tttaacggat atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca
    39301 atctcgtgta ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc
    39361 atccatagat tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc
    39421 ataaagtgag gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag
    39481 agaatcctta tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat
    39541 ttacctttta tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag
    39601 ataagaaata atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt
    39661 gactccgctt atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt
    39721 tatttctaca ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc
    39781 attagttact ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg
    39841 actgccaatc tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg
    39901 agaataatca tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt
    39961 gtttacaaca atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga
    40021 aacagttaaa gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt
    40081 attgtatctt ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg
    40141 caagcttaag atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata
    40201 ccatggcacc aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt
    40261 ttctatgttt gttaattttt taaataaaat caggggaata cataaagata acagaccaac
    40321 tagtcccatt gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac
    40381 attagatgca tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat
    40441 ttgttcaaac atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact
    40501 aaataatttt ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg
    40561 aataaaaatg ataattaatg ctgtactcat taatgttgat agtacatggc tgtacactag
    40621 caaactcata ctcaatgcta gctttacaga cggatacttt tcttcttttc cagagatgat
    40681 ttgataagca taaaataata cagggataac aagtgttatt gcgatagctt cggcaattgc
    40741 ggaacgatag tagatgtcta ctgtccgata agcagagaaa atatagagac aactaaataa
    40801 aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta cgagtgttgt
    40861 acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt ttgtcatcca
    40921 atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta caccatatcc
    40981 agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata accccttgat
    41041 tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat ttttgaaaaa
    41101 gatgaaagaa tatatactac taactataat taatccactc aatattacta cttttttatt
    41161 tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct ttcaacttat
    41221 taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga atactgatcc
    41281 ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta ttttctccag
    41341 catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg actttaccgt
    41401 ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt tgctttttgt
    41461 tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct ttttcccacg
    41521 attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc gcttctgttg
    41581 gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt gtttcttcat
    41641 tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct tttgctgcta
    41701 tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa tattttttat
    41761 tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag atttggatac
    41821 ctaagatacg ccaaggaaac tggagcaagc ttatcggtgt tttttgaaac aaaaaccatg
    41881 gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg attttttctt
    41941 ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat cctaataacc
    42001 ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct gaaatagaaa
    42061 gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa ttcatctgct
    42121 gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca gcctttacta
    42181 aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac aaacgaacta
    42241 taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga gtatacacaa
    42301 gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat atcaatccat
    42361 aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca attgcggatc
    42421 gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta aataaaaaag
    42481 cagcatctat ctttttaaaa aacttttctc cataataaaa a
//
LOCUS       DACDVT010000018        41896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDVT010000018 DACDVT010000000
VERSION     DACDVT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.018"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..409
                     /locus_tag="IWL52_000966"
     CDS             134..409
                     /locus_tag="IWL52_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9777547.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IWL52_000967"
     CDS             415..1182
                     /locus_tag="IWL52_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9777548.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IWL52_000968"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IWL52_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9777549.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IWL52_000969"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IWL52_000969"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9777550.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IWL52_000970"
     CDS             5326..5628
                     /locus_tag="IWL52_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9777551.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IWL52_000971"
     CDS             5631..6794
                     /locus_tag="IWL52_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9777552.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IWL52_000972"
     CDS             6820..10248
                     /locus_tag="IWL52_000972"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9777553.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IWL52_000973"
     CDS             10269..11393
                     /locus_tag="IWL52_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777554.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IWL52_000974"
     CDS             11383..11820
                     /locus_tag="IWL52_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9777555.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IWL52_000975"
     CDS             11804..12142
                     /locus_tag="IWL52_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9777556.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IWL52_000976"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IWL52_000976"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9777557.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IWL52_000977"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IWL52_000977"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9777558.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IWL52_000978"
     CDS             13299..14342
                     /locus_tag="IWL52_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9777559.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IWL52_000979"
     CDS             14501..15229
                     /locus_tag="IWL52_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9777560.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IWL52_000980"
     CDS             15345..15590
                     /locus_tag="IWL52_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9777561.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IWL52_000981"
     CDS             15587..15805
                     /locus_tag="IWL52_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9777562.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IWL52_000982"
     CDS             15941..16480
                     /locus_tag="IWL52_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9777563.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IWL52_000983"
     CDS             16473..18746
                     /locus_tag="IWL52_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9777564.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IWL52_000984"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IWL52_000984"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9777565.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IWL52_000985"
     CDS             complement(19919..20173)
                     /locus_tag="IWL52_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9777566.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="IWL52_000986"
     CDS             complement(20573..20842)
                     /locus_tag="IWL52_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777567.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="IWL52_000987"
     CDS             complement(21312..21767)
                     /locus_tag="IWL52_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9777568.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="IWL52_000988"
     CDS             complement(21905..23833)
                     /locus_tag="IWL52_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9777569.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="IWL52_000989"
     CDS             complement(24030..24863)
                     /locus_tag="IWL52_000989"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9777570.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="IWL52_000990"
     CDS             complement(25055..25759)
                     /locus_tag="IWL52_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9777571.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGAIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="IWL52_000991"
     CDS             complement(25782..26408)
                     /locus_tag="IWL52_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9777572.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="IWL52_000992"
     CDS             26734..29109
                     /locus_tag="IWL52_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9777573.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="IWL52_000993"
     CDS             complement(29133..29624)
                     /locus_tag="IWL52_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9777574.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="IWL52_000994"
     CDS             29811..30731
                     /locus_tag="IWL52_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9777575.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="IWL52_000995"
     CDS             30885..31229
                     /locus_tag="IWL52_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9777576.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="IWL52_000996"
     CDS             complement(31419..31919)
                     /locus_tag="IWL52_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9777577.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="IWL52_000997"
     tRNA            32447..32530
                     /locus_tag="IWL52_000997"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..33492)
                     /locus_tag="IWL52_000998"
     CDS             complement(32854..33492)
                     /locus_tag="IWL52_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9777578.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            33827..34261
                     /locus_tag="IWL52_000999"
     CDS             33827..34261
                     /locus_tag="IWL52_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9777579.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            34284..34604
                     /locus_tag="IWL52_001000"
     CDS             34284..34604
                     /locus_tag="IWL52_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777580.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            34745..34936
                     /locus_tag="IWL52_001001"
     CDS             34745..34936
                     /locus_tag="IWL52_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777581.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(35007..35264)
                     /locus_tag="IWL52_001002"
     CDS             complement(35007..35264)
                     /locus_tag="IWL52_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9777582.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            35422..35685
                     /locus_tag="IWL52_001003"
     CDS             35422..35685
                     /locus_tag="IWL52_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777583.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            35709..35897
                     /locus_tag="IWL52_001004"
     CDS             35709..35897
                     /locus_tag="IWL52_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777584.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(35980..36165)
                     /locus_tag="IWL52_001005"
     CDS             complement(35980..36165)
                     /locus_tag="IWL52_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777585.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            36380..36751
                     /locus_tag="IWL52_001006"
     CDS             36380..36751
                     /locus_tag="IWL52_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9777586.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(36901..36973)
                     /locus_tag="IWL52_001007"
     tRNA            complement(36901..36973)
                     /locus_tag="IWL52_001007"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36938..36940),aa:Gln,seq:ctg)
     gene            complement(37019..37447)
                     /locus_tag="IWL52_001008"
     CDS             complement(37019..37447)
                     /locus_tag="IWL52_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777587.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(37507..38265)
                     /locus_tag="IWL52_001009"
     CDS             complement(37507..38265)
                     /locus_tag="IWL52_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9777588.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            38560..38757
                     /locus_tag="IWL52_001010"
     CDS             38560..38757
                     /locus_tag="IWL52_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777589.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            38863..39345
                     /locus_tag="IWL52_001011"
     CDS             38863..39345
                     /locus_tag="IWL52_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777590.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(39403..39999)
                     /locus_tag="IWL52_001012"
     CDS             complement(39403..39999)
                     /locus_tag="IWL52_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9777591.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            40386..41852
                     /locus_tag="IWL52_001013"
     CDS             40386..41852
                     /locus_tag="IWL52_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9777592.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        13829 a         7134 c         8482 g        12451 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagatg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcgcacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttc cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagtaga tcaggttaat
    33301 atcagaaatt gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt
    33361 ttgagaaatc gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc
    33421 agtcgttccc atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt
    33481 aagtgatgtc atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg
    33541 aggaatactt taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca
    33601 aatccacgtt ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc
    33661 acataatgca gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa
    33721 taacttgaaa gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt
    33781 atttttgctt gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata
    33841 ccagaacatc atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc
    33901 ccttgactta gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt
    33961 tgagctgacc catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac
    34021 agaagtagcc atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca
    34081 cgcaatagtt gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga
    34141 aaatattgat ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt
    34201 tggatcgacc gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta
    34261 atgagataag ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa
    34321 aaatagaaat tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg
    34381 ctttagtaaa gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac
    34441 aagataaagg attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg
    34501 ataaatttat ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat
    34561 tagataaatt aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc
    34621 agcctttaga aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa
    34681 actagaataa tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag
    34741 gcaaatggaa gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata
    34801 tgaagaagcg aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat
    34861 cgcacaagaa tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt
    34921 tggtataaca gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc
    34981 gttttctttt taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa
    35041 ataataagaa aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt
    35101 gtttcccaat gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc
    35161 agtaaaactg aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata
    35221 ataaaaaaag gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata
    35281 aataaagtcc atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat
    35341 ttatccggta aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt
    35401 gaaagaagga gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc
    35461 aataatattc gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg
    35521 aataaaataa atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga
    35581 agttattata gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat
    35641 ctagatacca aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga
    35701 ggaaaaaaat gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa
    35761 agattgatgt aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga
    35821 atcgtaaata tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc
    35881 cgacacgttt attttagtat gaagtattca gaaaagaacc aaaggaggac gttgcagcag
    35941 cattgtcctc ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa
    36001 tttgtttttg acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata
    36061 aggtcctaat tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt
    36121 cttaatcatt tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt
    36181 tgctatcaac atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca
    36241 ataatatcaa ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag
    36301 gagaaaaatc tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat
    36361 ataacaagga ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag
    36421 tggtattttg ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg
    36481 tatacttgat cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa
    36541 accagaggaa gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa
    36601 tgggaaagaa cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt
    36661 gaaaattagc aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt
    36721 tccggagaaa gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga
    36781 ttttattatt cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa
    36841 tccccctgca aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa
    36901 ttgggatggc agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt
    36961 tggctacatc ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt
    37021 atttttcttg atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt
    37081 ctaatccagc aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat
    37141 attctttgac aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa
    37201 agcgagtata ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact
    37261 cttgatctgc gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg
    37321 tagcaggtct gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct
    37381 tgcagtcacg gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt
    37441 tttccattat ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt
    37501 gaattactag cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga
    37561 aaaatcgttg atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt
    37621 ttctttgtca tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc
    37681 attatttgtg gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac
    37741 agaagtccag acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc
    37801 ggtttttgga aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg
    37861 gggaacatag acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt
    37921 aggatcgcca tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa
    37981 ctggttttta gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc
    38041 acagccattt tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac
    38101 ttctgctttc tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat
    38161 tgtatgttct ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc
    38221 tgtgccccct agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt
    38281 tcagctgttt atctttccta ttataaatgt atttttattg gaaatggaag tattatattt
    38341 taatatgtag gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct
    38401 tctaaataca acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa
    38461 ggttgatttt taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg
    38521 taatgtggag aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat
    38581 ttaggagaac aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt
    38641 gtatcaaaaa tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa
    38701 gaattattag atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga
    38761 aaacaattgg acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt
    38821 cttttcctat tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg
    38881 gttctattgt ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa
    38941 attatttatc ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca
    39001 ctagtcattt ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc
    39061 ttgtcagcaa tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga
    39121 cttgttttga atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta
    39181 ttatatcagc caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga
    39241 ggaccgatgc tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc
    39301 ttaaacgatg aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga
    39361 caagatgttt gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa
    39421 gcgatcggca tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac
    39481 taattgcgaa cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc
    39541 aataactggg ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca
    39601 agtattagct gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata
    39661 taattcaaat tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt
    39721 accataacca tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg
    39781 agtcaatggg cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa
    39841 aaggataact gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc
    39901 tttgatcaaa gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat
    39961 tgaacggcgg tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt
    40021 tgcgttttgc atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag
    40081 taaacagata tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat
    40141 cgttatgtaa ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt
    40201 atttgaaaaa aacagctgat atataattat taaatctcta tcttcttttt tttatgatat
    40261 tgccattaaa aattacttgc ttttatagaa aattagttta tatttcttaa ataagttttt
    40321 tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat
    40381 agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt
    40441 tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct
    40501 attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag
    40561 ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat
    40621 ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg
    40681 gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga
    40741 ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt
    40801 caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag
    40861 gggcattatt agcaccacta atcgttggta caatcggtca agaatataac tatcatttag
    40921 ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac
    40981 gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta
    41041 aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg
    41101 cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga
    41161 ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag
    41221 aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat
    41281 tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc
    41341 tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta
    41401 ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag
    41461 tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc
    41521 cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct
    41581 tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat
    41641 tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt
    41701 cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact
    41761 tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac
    41821 caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt
    41881 atttgcagca gttcct
//
LOCUS       DACDVT010000019        40826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDVT010000019 DACDVT010000000
VERSION     DACDVT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.019"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IWL52_001014"
     CDS             33..656
                     /locus_tag="IWL52_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777593.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="IWL52_001015"
     CDS             1106..2155
                     /locus_tag="IWL52_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9777594.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="IWL52_001016"
     CDS             2152..3033
                     /locus_tag="IWL52_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777595.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="IWL52_001017"
     CDS             3036..3806
                     /locus_tag="IWL52_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9777596.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="IWL52_001018"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="IWL52_001018"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9777597.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="IWL52_001019"
     CDS             5117..6280
                     /locus_tag="IWL52_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9777598.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="IWL52_001020"
     CDS             6397..6807
                     /locus_tag="IWL52_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9777599.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="IWL52_001021"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="IWL52_001021"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9777600.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9347..9664
                     /locus_tag="IWL52_001022"
     CDS             9347..9664
                     /locus_tag="IWL52_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777601.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9824..10060
                     /locus_tag="IWL52_001023"
     CDS             9824..10060
                     /locus_tag="IWL52_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9777602.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="IWL52_001024"
     CDS             10057..10455
                     /locus_tag="IWL52_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9777603.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="IWL52_001025"
     CDS             complement(11122..12066)
                     /locus_tag="IWL52_001025"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9777604.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="IWL52_001026"
     CDS             complement(12362..13306)
                     /locus_tag="IWL52_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9777605.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="IWL52_001027"
     CDS             13527..14741
                     /locus_tag="IWL52_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9777606.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="IWL52_001028"
     CDS             14763..16778
                     /locus_tag="IWL52_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAP9777607.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="IWL52_001029"
     CDS             complement(16861..18234)
                     /locus_tag="IWL52_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9777608.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="IWL52_001030"
     CDS             complement(18260..18544)
                     /locus_tag="IWL52_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9777609.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="IWL52_001031"
     CDS             complement(18546..20561)
                     /locus_tag="IWL52_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9777610.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21045)
                     /locus_tag="IWL52_001032"
     CDS             complement(20740..21045)
                     /locus_tag="IWL52_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9777611.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21134..22165)
                     /locus_tag="IWL52_001033"
     CDS             complement(21134..22165)
                     /locus_tag="IWL52_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9777612.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22372..23247
                     /locus_tag="IWL52_001034"
     CDS             22372..23247
                     /locus_tag="IWL52_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9777613.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23399..24241)
                     /locus_tag="IWL52_001035"
     CDS             complement(23399..24241)
                     /locus_tag="IWL52_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9777614.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24333..26210)
                     /locus_tag="IWL52_001036"
     CDS             complement(24333..26210)
                     /locus_tag="IWL52_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9777615.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26207..27628)
                     /locus_tag="IWL52_001037"
     CDS             complement(26207..27628)
                     /locus_tag="IWL52_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9777616.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27631..31020)
                     /locus_tag="IWL52_001038"
     CDS             complement(27631..31020)
                     /locus_tag="IWL52_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9777617.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31277..32668
                     /locus_tag="IWL52_001039"
     CDS             31277..32668
                     /locus_tag="IWL52_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9777618.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32741..33388)
                     /locus_tag="IWL52_001040"
     CDS             complement(32741..33388)
                     /locus_tag="IWL52_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9777619.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33396..34082)
                     /locus_tag="IWL52_001041"
     CDS             complement(33396..34082)
                     /locus_tag="IWL52_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777620.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34088..35161)
                     /locus_tag="IWL52_001042"
     CDS             complement(34088..35161)
                     /locus_tag="IWL52_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9777621.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35383..36057
                     /locus_tag="IWL52_001043"
     CDS             35383..36057
                     /locus_tag="IWL52_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9777622.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36062..37402
                     /locus_tag="IWL52_001044"
     CDS             36062..37402
                     /locus_tag="IWL52_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9777623.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37551..38537)
                     /locus_tag="IWL52_001045"
     CDS             complement(37551..38537)
                     /locus_tag="IWL52_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9777624.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39261..40292
                     /locus_tag="IWL52_001046"
     CDS             39261..40292
                     /locus_tag="IWL52_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9777625.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40401..>40826
                     /locus_tag="IWL52_001047"
     CDS             40401..>40826
                     /locus_tag="IWL52_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9777626.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        12672 a         7419 c         7664 g        13071 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgattta tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcacatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaaac cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    21001 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    21061 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    21121 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    21181 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    21241 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    21301 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    21361 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    21421 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    21481 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    21541 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    21601 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    21661 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    21721 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    21781 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    21841 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    21901 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    21961 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    22021 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    22081 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    22141 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    22201 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    22261 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    22321 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    22381 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    22441 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    22501 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    22561 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    22621 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    22681 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    22741 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    22801 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    22861 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    22921 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    22981 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    23041 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    23101 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    23161 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    23221 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    23281 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    23341 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    23401 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    23461 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    23521 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    23581 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    23641 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    23701 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    23761 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    23821 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    23881 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    23941 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    24001 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    24061 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    24121 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    24181 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    24241 ttcgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    24301 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    24361 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    24421 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    24481 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    24541 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    24601 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    24661 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    24721 tgatcacgaa cgacgaattc tgctccagca agtgtttgat ctgatgtaac gtcaccatct
    24781 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    24841 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    24901 ttcaaatgca tatagtaaac aaatttcagc gtaccaccag gagtgagtga aggaatataa
    24961 ttcgagtcaa ctgaaaccgt gaatccgttt gtttgttcag tgacagaata atttgttgga
    25021 tcgatttctt tatccccatc atacaagaca tacgcatacg ttcctgaagt ttcatgatca
    25081 aatgtcaaag cggcgtcatg agtatcaatg agtttgaatg tcgtgtactt gttttgtgtt
    25141 ccttctttat cagcgatacc taaaggaata ttgacagaga tctcatattg gattttttct
    25201 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt ggacgtatca
    25261 tttttgattg tcttttctac aggagtttga ctatttgcca agatctcaaa cggcgtgttt
    25321 gtttgattat caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    25381 ccaacctcta aatgattgac gatcaactga cctttttcaa tcgttgcctc ggcaaagtca
    25441 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatccgtt
    25501 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtcttcct
    25561 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    25621 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    25681 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    25741 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    25801 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    25861 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    25921 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    25981 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    26041 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    26101 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    26161 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    26221 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    26281 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    26341 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    26401 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    26461 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    26521 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    26581 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    26641 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgatac
    26701 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    26761 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    26821 tcgaaaataa aacatcaaat ccgtgaggtt ttttgtgtat gacaaagcct tctgtcagct
    26881 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    26941 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    27001 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    27061 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    27121 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    27181 cgacttctgg tgctttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    27241 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    27301 ctgcggtaaa cgtaccggaa gacgcaccgt tttttgcgat ctctgtttgt gcttcttcta
    27361 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    27421 tgacaccatt taatcctcga tacgtttgta ataacgcttt ttcttgtccg tcatttggat
    27481 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    27541 ctgtttctgt ttctgttgct gcctgagtaa aagctggttt aaatagtaac aagaagagac
    27601 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    27661 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    27721 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    27781 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    27841 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    27901 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    27961 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    28021 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    28081 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    28141 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    28201 ggcaggacca gctgtttcaa cgactcggta tttaccaggt gtgagtgaat caaaactggc
    28261 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    28321 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    28381 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    28441 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    28501 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    28561 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    28621 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaagacag caccgatgag
    28681 attcttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    28741 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    28801 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    28861 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacga
    28921 atttgtagta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    28981 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    29041 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    29101 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    29161 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    29221 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    29281 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    29341 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    29401 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    29461 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    29521 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    29581 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    29641 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    29701 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    29761 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    29821 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    29881 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    29941 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    30001 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    30061 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    30121 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    30181 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    30241 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    30301 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    30361 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    30421 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    30481 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    30541 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    30601 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    30661 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    30721 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    30781 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    30841 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    30901 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    30961 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    31021 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    31081 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    31141 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    31201 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    31261 atgaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    31321 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    31381 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    31441 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    31501 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    31561 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    31621 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    31681 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    31741 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    31801 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    31861 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    31921 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    31981 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    32041 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    32101 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    32161 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    32221 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    32281 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    32341 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    32401 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    32461 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    32521 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    32581 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    32641 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    32701 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    32761 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    32821 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    32881 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    32941 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    33001 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    33061 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    33121 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    33181 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    33241 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    33301 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    33361 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    33421 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    33481 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    33541 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    33601 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    33661 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    33721 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    33781 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    33841 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    33901 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    33961 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    34021 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    34081 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    34141 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    34201 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    34261 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    34321 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    34381 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    34441 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    34501 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    34561 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    34621 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    34681 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    34741 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    34801 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    34861 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    34921 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    34981 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    35041 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    35101 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    35161 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    35221 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    35281 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    35341 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    35401 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    35461 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    35521 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    35581 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    35641 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    35701 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    35761 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    35821 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    35881 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    35941 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    36001 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    36061 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    36121 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    36181 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    36241 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    36301 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    36361 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    36421 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    36481 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    36541 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    36601 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    36661 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    36721 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    36781 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    36841 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    36901 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    36961 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    37021 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    37081 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    37141 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    37201 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    37261 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    37321 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    37381 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    37441 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    37501 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    37561 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    37621 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    37681 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    37741 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    37801 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    37861 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    37921 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    37981 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    38041 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    38101 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    38161 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    38221 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    38281 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    38341 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    38401 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    38461 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    38521 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    38581 acttataaat ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg
    38641 attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata
    38701 aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg
    38761 tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat
    38821 tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac
    38881 tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa
    38941 taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa
    39001 gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag
    39061 ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta
    39121 aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta
    39181 agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac
    39241 actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa
    39301 agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt
    39361 ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt
    39421 tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag
    39481 attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga
    39541 accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt
    39601 cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc
    39661 ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac
    39721 ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag
    39781 ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt
    39841 gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc
    39901 agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag
    39961 cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt
    40021 ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga
    40081 gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt
    40141 atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc
    40201 tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat
    40261 tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat
    40321 aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa
    40381 caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta
    40441 cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac
    40501 tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt
    40561 tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt
    40621 ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag
    40681 tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt
    40741 tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa
    40801 atggaacaaa atcaaaacag attcct
//
LOCUS       DACDVT010000020        38326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDVT010000020 DACDVT010000000
VERSION     DACDVT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.020"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="IWL52_001048"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="IWL52_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9777627.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="IWL52_001049"
     CDS             complement(2204..4390)
                     /locus_tag="IWL52_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9777628.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IWL52_001050"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IWL52_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9777629.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="IWL52_001051"
     CDS             complement(4612..5052)
                     /locus_tag="IWL52_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9777630.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="IWL52_001052"
     CDS             complement(5126..5665)
                     /locus_tag="IWL52_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9777631.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="IWL52_001053"
     CDS             complement(6280..7746)
                     /locus_tag="IWL52_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9777632.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="IWL52_001054"
     CDS             complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="IWL52_001054"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9777633.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10280..10459)
                     /locus_tag="IWL52_001055"
                     /pseudo
     CDS             complement(10280..10459)
                     /locus_tag="IWL52_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(10662..11021)
                     /locus_tag="IWL52_001056"
     CDS             complement(10662..11021)
                     /locus_tag="IWL52_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9777634.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11051..11392)
                     /locus_tag="IWL52_001057"
     CDS             complement(11051..11392)
                     /locus_tag="IWL52_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777635.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11389..12063)
                     /locus_tag="IWL52_001058"
     CDS             complement(11389..12063)
                     /locus_tag="IWL52_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9777636.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="IWL52_001059"
     CDS             complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="IWL52_001059"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9777637.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14578..15768)
                     /locus_tag="IWL52_001060"
     CDS             complement(14578..15768)
                     /locus_tag="IWL52_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9777638.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15789..16316)
                     /locus_tag="IWL52_001061"
     CDS             complement(15789..16316)
                     /locus_tag="IWL52_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9777639.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16363..19152)
                     /locus_tag="IWL52_001062"
     CDS             complement(16363..19152)
                     /locus_tag="IWL52_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9777640.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19299..19499)
                     /locus_tag="IWL52_001063"
     CDS             complement(19299..19499)
                     /locus_tag="IWL52_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9777641.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19951..20271)
                     /locus_tag="IWL52_001064"
     CDS             complement(19951..20271)
                     /locus_tag="IWL52_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777642.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20655..21773)
                     /locus_tag="IWL52_001065"
     CDS             complement(20655..21773)
                     /locus_tag="IWL52_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777643.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22244..23551
                     /locus_tag="IWL52_001066"
     CDS             22244..23551
                     /locus_tag="IWL52_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9777644.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23670..24869
                     /locus_tag="IWL52_001067"
     CDS             23670..24869
                     /locus_tag="IWL52_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9777645.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24896..26164)
                     /locus_tag="IWL52_001068"
     CDS             complement(24896..26164)
                     /locus_tag="IWL52_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9777646.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26327..26974)
                     /locus_tag="IWL52_001069"
     CDS             complement(26327..26974)
                     /locus_tag="IWL52_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9777647.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27382..27885)
                     /locus_tag="IWL52_001070"
     CDS             complement(27382..27885)
                     /locus_tag="IWL52_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9777648.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28008..28772)
                     /locus_tag="IWL52_001071"
     CDS             complement(28008..28772)
                     /locus_tag="IWL52_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9777649.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28880..29155
                     /locus_tag="IWL52_001072"
     CDS             28880..29155
                     /locus_tag="IWL52_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9777650.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29593..30543
                     /gene="yidC"
                     /locus_tag="IWL52_001073"
     CDS             29593..30543
                     /gene="yidC"
                     /locus_tag="IWL52_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9777651.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30722..31696)
                     /gene="lacD"
                     /locus_tag="IWL52_001074"
     CDS             complement(30722..31696)
                     /gene="lacD"
                     /locus_tag="IWL52_001074"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9777652.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(31693..32151)
                     /locus_tag="IWL52_001075"
     CDS             complement(31693..32151)
                     /locus_tag="IWL52_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9777653.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(32148..33611)
                     /locus_tag="IWL52_001076"
     CDS             complement(32148..33611)
                     /locus_tag="IWL52_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9777654.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(33593..34543)
                     /gene="pfkB"
                     /locus_tag="IWL52_001077"
     CDS             complement(33593..34543)
                     /gene="pfkB"
                     /locus_tag="IWL52_001077"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9777655.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            34686..35434
                     /locus_tag="IWL52_001078"
                     /pseudo
     CDS             34686..35434
                     /locus_tag="IWL52_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            35700..37178
                     /locus_tag="IWL52_001079"
     CDS             35700..37178
                     /locus_tag="IWL52_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9777656.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(37246..38301)
                     /locus_tag="IWL52_001080"
     CDS             complement(37246..38301)
                     /locus_tag="IWL52_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9777657.1"
                     /translation="MLITGAAGFIGSNLVLELLRTQSSIKIIGIDNMNDYYDVSIKNY
                     RLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLAAQAGVRYSITNP
                     DMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKKIPYSTEDKVDNQ
                     VSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLRAGDNI
                     QIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHIYNIGNNTPENLL
                     DFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSALERDFEFKPSTSL
                     RDGLRKFAQWYKSFYEV"
BASE COUNT        11153 a         8143 c         6651 g        12379 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga ggacgttcat
     8041 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
     8101 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
     8161 aagcacctaa ggcaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
     8221 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
     8281 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
     8341 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
     8401 tctttttatt gtttctttgt aagatttcct ctaaccgctg cactttatct tctggaagca
     8461 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
     8521 aatcgcccgt caacatgact gtgttcttca caccagcctt ttttaattga accaatgcgt
     8581 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
     8641 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
     8701 ccatcaattt gtgattacct gctaatactt gtttgccatc aacttcagct aagatccctt
     8761 cacctgcaaa ttcttggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
     8821 cagattgagc aatcggatgt gtagaaaact gttctgcact cgcaacatat tgcatgaata
     8881 tctttggatc gatttttgta tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc
     8941 cggttttatc gaataccact gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt
     9001 ttactaatac accgatttta cttgctcctc cgataccgcc aaagaaacta aggggtacgg
     9061 agatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga tagatccatt
     9121 cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac agctaataaa acggccaatc
     9181 ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca gctggtgctt
     9241 ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa ttttcataag
     9301 atttttctac ttgaactaaa aatggtttat ttttattgat aaatccactt aatactgttt
     9361 caccaacata tacagattta gggactgatt ctcctgtaag tgctgacgta tcgactaatg
     9421 attctccttc gatgatcgtt ccatctaacg gtacacgttc accaggcttc acaagtacaa
     9481 actgaccgat ctgcacgtct tcagggttca cggtcatcaa tccttccgtc gtctgcacgt
     9541 gagctgtttc tggacggatc gccatcaatt cgcgaatcga ttttcttgaa tgactgactg
     9601 ccaatccttg gaaatactcg ccgatttgat aaaatagcat cactgctacg gcttctggat
     9661 attcaccgat caacatagcg ccaatcgtag caacagacat aagaaaattc tcatcaaaga
     9721 tttttccttt gacgatattc atcactgcct tcttcactac atcatatcct atcagcagat
     9781 aaatcaataa ataccctatc aagcgaattg gttcattaaa atcaaataat agcaataaag
     9841 cgatacctgc aagggaaaat aaaatccgga acagcgtatt tctctctttt tcatttccat
     9901 gttcagatac tttttcctta tctatttctg ttactttgac atctggttct aattccttca
     9961 ctactttttt tatttcttgt tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta
    10021 gttttccaga agtgaagttt accgttgctt ccttgactcc attgatcttc tgaactcctt
    10081 tttcgatttt cattgcacag ttcgcacaat ctaaaccttc taatctgtaa cttttcattt
    10141 taatgacctc ctcgcctcaa catatgaata tctcttcata actaattata aatgaacggt
    10201 tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct
    10261 tccatacaga tgttttggct tattctttct ttttcttttc gatcattgcc actgctctta
    10321 tccaacccgc acttgccttc ttgatcttga caggatagca actaagtttg aagcctgttg
    10381 ctggcaattt gtctaaattc attaattttt cgataggaca atatcttttt tcaatccctg
    10441 caaaatgtcc ttcccagata ttcgaagctc cactacgctt tcaaaaatat agaaagagtt
    10501 ctttaatgaa aattatttca actttccata aacagtaacc ccatataaat aaaagacgta
    10561 aaagtcacat ggaagaactt atacaggaga acgcatagag tcaggtgaaa tacatgtatc
    10621 ccacctgact ctatgcttta tctaaccttt attattctct tttatacaat ttctgcaccg
    10681 aatggcatca acgccaatcg agcaagtttg aaagattgct tggcaaaagg tatccctatg
    10741 atcgtgatac atagaaggat gccgctgacg acatgtgcga gagcaattgg taggcctgaa
    10801 acgatcagcc agatgatatt aacaatcaat gacatgccag actggtgata aattacttct
    10861 ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac attgcatacc gattgggata
    10921 cctatgatcg tgatacacca aagtatcccc gctgctaacc agctaagacc accgattagt
    10981 ccgccgaata taaaccagat gacatttcct aaacaactca ttgtatttct ccttctatta
    11041 ctgtttttga ttaaaactgg gcaaaaaatg attttattct tcttttttta gcttccagta
    11101 tcaaatattc tgcaaatttt ttttggattt cttccgtatt ttcttttgtt actaatgtgt
    11161 gttggattag ttttcctgat ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg
    11221 cttgcgcaaa tccgataatc cgtttttctt ttgttacagc aacgagcaat agtttattct
    11281 tatcatttag gtcagctgcc gtttttgctt ttacgaatga ttctggatga tcagtaatca
    11341 gctgattgat ttgaggtaga tcttctttta ctgcccaccg tatttccatc atttctcact
    11401 tttctttcgc tcatttttat ctccactaaa aaaatcgact gcggcaattg catctatcca
    11461 gctttgtgca atgatctcat gtccgccaat cgttgggtgg accccatctt ctcttgtata
    11521 tttcgaaaaa ccataaacaa tccctaaagc attgaatata ccatctaaag gaaccagcaa
    11581 tgcgccatat tccttagcaa gtttacgaac aatttggatt ttagggtcta agtctttacg
    11641 ccagccatga cggtcgtcgg ttataggaag tacaaacggc tccatcaaga tgatgttgca
    11701 gattcccact gtttgaagtt catttagtaa ttttcgataa acggattcaa attgctgagc
    11761 agatttctca gttccgaata tagcctgatc gctagtatta tgccaggtat cattgattcc
    11821 gatcaaaatc gatacatagt caggttgtaa agctaaacaa tcttcttgcc agcgtacttg
    11881 cagatctgca attttgtctc ctcctatccc acggttcatg cattcaaatt ccatcgttgg
    11941 atattgggag attagttttc cggcaatcaa aagtggataa cctttaccta gatcagttgc
    12001 ttctgctcgg tctcttcctg cgtctgtaat gctatcaccg ataaataata tacgtttttt
    12061 catttgtcaa aaactccttt ttgaataaaa cgatgatttt cttccttata ttatcccata
    12121 agccctatca agacgaaaga gaaaatatat gtataaaaaa tgacaccagc ctagtccctt
    12181 caaacaattg aatgaacgct catcaagtgt ctagtttttt atgctatttt tttcttaacg
    12241 tgccggattc cccttgattc cttcgttgtt tggtccagag agatcgatca cacctaagct
    12301 tccgtcaatc agaccactga tgacgccatc agaacatata tttataggct catctaagac
    12361 ttctagtaaa tgtcccttta ctaatatctc ttctagattg cttgggatag attggtcata
    12421 actttatctg gtccgatgat ttcaagggtg aattgaactt acatatggat tattgcatgc
    12481 tgaaaatacg gacctactcc atcttcgaat gttactatta gtttgttttc aggtgctaga
    12541 tgctgcttta tttcaacagc agaagcatca tcaaatcaga tttccatttg aattaccttc
    12601 tttgtaaatg tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt
    12661 tcttttgctt tcttctcttg tctgctggaa acttccttta tcccgtattt taaaaaaaga
    12721 actgcgagag ttcgcagttc ttttttagaa taaacggtgc tctccggcca actcctcggt
    12781 cacaagtcac aatagtcatc aaacatgaaa aactgttttt tgaaattact ccttgtgact
    12841 cggagctgaa cggcaggcgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    12901 cgatattagc taaaaaaacg gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    12961 tactcaggtc aaggcactga tttcacaacc tcttccttaa acggtgttca aaagccagtt
    13021 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc catttccaca aaatttttct
    13081 taatactcga aactaaacag ctttttcaca acctctccac taaacggtat tcaaaagctt
    13141 acatctgcgg taataagtcc cgccaattca aaaacatgaa cagctgtttt tgcttggctg
    13201 ttctaattac ttgatgcaga acagcttttt catcacctct tatggataaa ttcggttcaa
    13261 caaacgtggg aatggaatag cttcacgcac atggtcgata ccagcaatcc aagtgactgt
    13321 tcgttcaagg cctagaccaa aaccagagtg cggaacagaa ccgtatttac gtagatcaag
    13381 ataccatgaa tattcttttt catccaaacc gtgattacgg atttgttcta gcaagaaatc
    13441 atagtccgtt gcccgttcgc ttccgccaat aatttcaccg taaccttctg gtgcgatcat
    13501 atctgcacaa attaccacgt cttctcttgt tggatgtggt ttcatgtaga atggcttgat
    13561 agcttttgga tagtttagga taaagacagg ctgttcgaat gaattcgcaa tgaacgtttc
    13621 atgaggtgaa ccaaagtctt cgccccattc gatgtcatca aatccatttt tcttcagtaa
    13681 ttcgatcgca tcatcataag taatacgagg gaatggcaat tttgtataag tttttaagac
    13741 ttctttgtct cgtcctagta catctaatgc gtattcgcaa tgatctaaaa cattttgtac
    13801 taagaatgct acatattgtt cctgtacttc taaactttct tcttgatgag tgaaagccat
    13861 ttctggttcg atcatccaga attcaatcaa atgacgtcgt gtttttgatt tttcagcacg
    13921 gaaagtcgga ccgaatgaaa agacttttcc gaaagccata gcagcagctt ccatgtataa
    13981 ttggccactt tgagataaat aagctttttg atcaaagtaa tttgtttcga atagatccgt
    14041 agtgccttct ggtgcggaac cagtcaagat cggcggatcg attttgacaa aatgatgttg
    14101 attaaagaat tcatacgttg cacgaatgat ttcatttctg atctgcataa tcgcatgttg
    14161 tcttgaagaa cgtagccata aatgacgatg atccatcaag aagtctgttc cgtgttcttt
    14221 tggtgtgatc ggataatcgt ggctctcgct taccacttcc acatttgtaa ttccgatttc
    14281 ataaccaaat tttgaacggc tgtcttcacg gatttctcca gttactaaca cggatgtttc
    14341 ttggtgcaga tttttagctg tttggaaaac ttcttccggc acttcgcttt ttacaactac
    14401 accttggaaa aaggctgttc cgtctcttaa ttgtaaaaat gcgatttttc cgcttgatcg
    14461 cttatttgcg acccaagcgc cgattgttac tgtttctccc acatggtttt tcgaatcgat
    14521 gatttgaatt tgttccacgt atgactctcc ttttatattc cttgaatttc ttcaggatta
    14581 attttgactt ttactctcaa taaattgctt gattctgcgt accgcttctt ctaacgtagc
    14641 gcgatcagtg gcataactca tgcggacatt ttctggtgcc ccaaagcctt cgccagtcac
    14701 aagcgctacc cctgtttctt ctaaaagatc ttctacccat tgtgtgacat tttcatattt
    14761 acacatgcgt agcgtttctt ttacattagg aaaaagataa aaagctcctt gtggcttttc
    14821 aagagaaaca cccggcagtt ctgccacaag cggatataac gtattcaggc gttcttcaaa
    14881 ggcgttccgc atttcttcca ctgtatcttg ctcgcctttc aatgcttcaa cagctgcata
    14941 ttgactgact gctgtcggat tgctagttga ttgggaagca acagcgatca ttccattgat
    15001 gatcacagga tttcccaccg cataaccaat gcgccagcct gtcatcgcat atgttttaga
    15061 cacgccgttg atgatgatgg tctgattttt gattgcttct gaaatcgttg caattggagt
    15121 aaactcattg ccattataaa ctaaacggcc ataaatatca tcagcaacta tcaaaatatc
    15181 attttttact gcccattcac cgattgcctg cagttcattc ttgctataaa tcattccagt
    15241 cggattcgat ggagaattga tgattacggc ttttgttttt tctgaacgtg cttgttctag
    15301 ctgctcaaca gtaactttcc agctattgga ttcttctcct ttcaaaaaga caggaagtcc
    15361 ttctgccagt tttacttgct caccgtaact gacccagtaa ggcacaggaa tgatgacttc
    15421 atcttgcggg tcaaggatcg tttggaaaag ggtatacaaa gcaaatttcg ctccgtctgt
    15481 tacgactgtt tgtgaaggtt catagacaag tccatagttt ttcttcagat aatcaacgat
    15541 tgcctgtcga agctctggga ttcctgctgt tggtgtataa tagctagctt tcccattgcg
    15601 gattgcttcg attgctgctt cttggatgtt ttctggtgtc gcaaaatccg gttctcccac
    15661 agtcaaactc agcacatctc tgcctttggc ttttaatgcc ttggcctttg ccgcagctgc
    15721 caaagtgaca gacggttcta atcgttttgt tctttttgat aactccatcc cattcattcc
    15781 tttacctttt aaattccctt gattgtcttg acttcatctc cagattcaaa gccaatcagg
    15841 taataattca gtttcccatt tgtttctttt cctactactt cccacacagg ctgatcatca
    15901 tacatcccta aagcagcttt ttcgatttgg atttcaggat gggccgcagc aattgtctgt
    15961 tttgcttcat tttcagtcag tccctttgtt tgatccaata cttttacttt ttcaccagat
    16021 ttaggaatga taacggcgat ttcttttcct ttatcatttg tacctgtcag tgaaaagtat
    16081 gtcttctttc gagtaaacca ataaaaatga tcggcttcct caagatttgc atatttcttt
    16141 gcgatttcta ctgcttcttt cttcgcctgc accgccggac gactggaacg caagtaaaaa
    16201 atcgagctga tcacgatgac cgctaataaa aatacgatca agccaagtaa cacgcgatta
    16261 atcgtttttt cctttgtctg ttttttcttc aagggtcgtc gctcctttat cttcattcct
    16321 tttcattata gcagatttta attatgaaaa aagacagctc ctctaacttc cttcttcatt
    16381 tttctttaag aattcatgga tatcctgata gatttcctcc atagctagct ctttgatcgg
    16441 tacttttttc ggtaatgcat ggatgatccg tgcaccatat tttgcagtaa ctagtcttcg
    16501 atccaaaata agcaatacgc cacgatctgt ctccgtcctt atcaagcgtc ctaatgcttg
    16561 gcgtaacttc aaagctgctt taggtacgga ttcctggtaa aaaggattga tcccctctga
    16621 ttctaaataa gcgttcctag ctttgaccat cgggcgaagc ggattatcga agggtaaacg
    16681 tgtgactaca acgatctgca aagcatctcc aggcaaatct acaccttccc aaaaactgtc
    16741 agcgccaaac aatacgcttt tttctgacaa aatgaagcgt ttcaaaagct tctcccggct
    16801 tccgccaaac ccttgagcta gtatttctcg tcctttttct agcatagaca gatgcatttt
    16861 ctgatagacc ttctgcaaaa catcatgaga agtgaataac acaagtattg gccgatcttc
    16921 ttgttcgatc agacctgtaa gtgctgtctc taaataatcg atatatgctt gattgctgac
    16981 ttgatggacg gcaggtgcct cttttggcac atagatcctt gcctgtttct cataatcata
    17041 aggagaggca atcactttaa gaggtgctgc ttcaatgcca agccgcttag agagatagtg
    17101 tcgattaggg ccgatcttca gcgttcctcc aatataaaga atacgatcat atcgaggata
    17161 ccaaacagtc gacggcaaca tagccgcttg aaaatcatgg agatgaaaaa tcatctgctg
    17221 cgctgtgttc ccaggaacga accaatggat caaatgatct cgccagtctt ccagccattg
    17281 atccatcagc aagtactgct tgtctaattg atcaaataag ctatataaac ttgctaaatc
    17341 gatccgcatc tggtttgtcc agctttcttg gataatctca aaagtcagac gcatttgtga
    17401 ctgtagctgc tggatctcct gataaaaaag ctgcaggtga tcaatggctg tcccagattc
    17461 cagtggcagc tgatccaaca tctcttttgt cgtcatgact tcttctggat actgtccgtt
    17521 aggttgcttg ccgctcacta aactcattaa tccttctgcc atacaaatct gtgcttcgca
    17581 tagcgcagaa agttcttctc gataaatcga taaccagcgt aaggcttcat gttctttcgg
    17641 taaatagctt tgtattcggt caaacagctg atttggttct tctaattgct ggatttgttt
    17701 atggaaatag ttaatactta atcgtttgtc atttactttt tcagcgattt ccggcaaatg
    17761 atgtacttca tcgatcaaca aaaatggact agctggaagc aatggttctt ttcgctgtgt
    17821 ttcttgtatt aaaaacgcat gattgacaat caaaaaatta ctttgcctga ttttcttttg
    17881 caagaaacga acaaagtctt gttcgtaaaa tgactgattt cttgctaaaa atccagtgcc
    17941 tctgtgcgcg atatctgcaa agaatgaatg gttcaaattt gttaaatgta attcatccaa
    18001 atcaccggtc ttcgtttgcg tcagccaaac taagaccgcc atttgataca aagcatattg
    18061 tttttgttct ggcggttcca caagtgtgcc tttaaaacgc tgtaaatcaa tatagtggcg
    18121 atagcttttg ataattacag gttgtatggg ctgatctagc aattgattca gcaatggaat
    18181 gtctttttct atgatctgct gctgcaaaac tagcgaaacg gtactgatga cagcgggttt
    18241 ttcaggtgta gccaaataac tcaatggcag cagataaccg attgttttac ccattcctgt
    18301 agctgcttcg atcatcaaat cttttgattc atcttttgtg aaatggtcgt agaccgcatt
    18361 catcagtctg ttctgttctt ttcgatacat cagtgtttta ccaaacattt tttcttttgc
    18421 ttgttttttt cttggatatg tccgctcgcc ataatggacg tatatgaata gttccacttc
    18481 ttttttctgc aaagctagcc catcgacgat ttctaatgag ggatcaagtg gctctggttt
    18541 ttctttcatt tcttcaatca catgataaat gaaacgactc gtatccatac ctgtgtgtct
    18601 acttaatcgc gcaatctgct ctaacgtgat gattggcaat tttctcatcc gttcttcgat
    18661 cagcaaaaga agttgacccg tcacttcggc atcactatct gcctgatgag gattctcatg
    18721 aataaatcca aggctttctg acaaatcagc taagcgaaag cttggttccg tcggcaagaa
    18781 aatttgtgca agttctactg tatcgatgcc tggtatcttc aaactaggaa gtccgcatcg
    18841 catgagttca tggttcaaaa aattataatc aaaataaata ttatgcgcga caaatatggt
    18901 gtctgccaaa agattgtaaa tcgtttgggc gacatcttcg aaataaggcg ctttttggac
    18961 gcgttgattc gtgatccctg ttaaatgctg gatttgtttt gaaatcttcc gccctggatg
    19021 aatatcgatc gaaaaacgtg aagtgatccg acctgattca atcatgacgc agccaaattg
    19081 gatgatccga tcttcttttg gattcgtccc agtcgtttcg atatcaacga ctgcataagt
    19141 tcttggtttc aaaatatctt cctcttctcc tgcctctaat gttcttttga aattatacta
    19201 caaagtgcag aaaaatactc tcatcgcttt ttaaagaaaa tagacaaaaa cgcccagaca
    19261 cgcaaatgtc tgggcgttta ttttttagta taggttaatt aagctttagt aacgtttgtt
    19321 gcttgaggtc cgcgttggcc ttcttcaaca tcgaaagtta ctgcttggcc ttcttctaaa
    19381 gttttgaatc cgtcgccttg gatagctgag aaatgaacga atacatcgtt tccgttttct
    19441 gcagtgatga atccaaatcc tttttctgag ttaaaccatt ttactgtacc tgtttccata
    19501 ataaaaatcc tccttgtgta tcaacacata ttttgcaatt acttgaatgg tgtatatctg
    19561 gaagatgttt atttgaaaca agctaccaca gattaccgaa caaaaactac tcctatagta
    19621 tatcataccg tttacaaaaa agctagagtt tttcgacttt ttttattaaa aaaagttttc
    19681 tcagcgatta cataaagtga aaaaccgaac tttttttaaa ataaacgaca tattgttcag
    19741 ttggtgtttt tttgctctga tgttcttctt taaactaaaa aaattattct ttctgactac
    19801 ttatcggttc tgtttcaacg gtcgaggctg gggaagattc tttagtaggt ctgatgttct
    19861 catggtattt ctgatagcct atgacagtta ccagaagaac aaggcaaacc aatacgatca
    19921 acgagattag tttttctttt ttgctcatat ctatacctca taaataacag gatgatgtcc
    19981 atcatatttc tgagtatgag taaaagcaaa agctgcagct gtagaatgta agaaatgcca
    20041 gcaactttca taatcacagg tcggacagta ccaaaagtaa ccaaatcttg gctgcaaaga
    20101 ccgttcttct gtttcaggaa tcgcagaatc aagatctgtt gtctgtacat aaacattttt
    20161 gtacagacgt gtgcctgtaa taggagaccg ctgtgtttct tcagcaaaaa ctggctgact
    20221 tgttaaactt agggatagaa acagcaaaca ccataacagt ctttttttca taaagacctc
    20281 cttatcgata agtttttccc aattaaaatc atatctcaag aacgcccgtt attcacattt
    20341 tgttcataaa tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga ccacgtcaca
    20401 acctcttttt taaacggtgt tccagaagta gcttcttcgg aaataagtcg aaatttcaca
    20461 aaaatttgag aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt
    20521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtattag ctcaaaaaac
    20581 ggaaaaatat gtgaaactat ttttccgttt gtttctctaa tactcaggtc aaaacactga
    20641 tttcacaacc tcttttaata acgaagaatc gctgtcagac tcttttcatc tggcgcatct
    20701 ttttgatcca aaataaacac tttcccgcca ttaatgatca catcatctgc taatgcattc
    20761 aacacttgac gccgatcata ttctgtttca ggatcttcac cgaaaccatc agctaagttt
    20821 gacgtagcga taaataaatg tgacactttt ccttctttcg cagctggaac gatatcaact
    20881 aactgatcga tgaatttctt atctaagagt ttattgtatt cttttgattc ttgagctgcg
    20941 agttcacttg acagcttctt cgccccttct tcgatatctc taaaggatag ctgcgcagga
    21001 gaagcagaaa tcgcaacgtc tgcacaataa tagggatttt tagcaatttt tttgaacatc
    21061 gtctgatttt ctggtaatgc aaataaataa atcggccatt gttcaggatt ttcgaaagaa
    21121 tccttcaagt acgtatcaac agcttggtaa tagttgaccc aatcgatctc tacttcttca
    21181 tcttttggat ttacgccatg gaaagttact ccatctcggg ctccgcctcc tccattagaa
    21241 gagaagttca aaccgccacc tgtcagttca gatcccaatg cagtaacgac atcaaccggt
    21301 gccccttctg gtagagatac ttcttttact tgtttgttgc tgacctcata caatttcatg
    21361 gagtcgcggt tcaatcccaa taaataataa gaatagttga actgcccatt tttgataata
    21421 gccagtaaat aaggaagatc acttacatag tattgatcgt ctaccgggat actcaaacga
    21481 tggatatagg tctggtcagc agttataatg accgctacac tcgtagttgc actacgccaa
    21541 aacgaaggat ctcctaacaa attttcaatt ttttcttgga acggggacca gtcatgttcg
    21601 gaatattttt tctcaaaacg tttctttgct tctttcgcaa aatttttgaa tttgatctga
    21661 tctttttcca catcttgatg ggcgacatgc gtattcagca tgatcgtgac gaatggtcct
    21721 ttagcgcctt ctgaagtcaa ttgtgtcaat aattctttat ccttttttgc cataaacgta
    21781 cccctcctaa aaaaatcatt cctttgtttc tatctaaagt ctaccacaac actatttttc
    21841 catacaataa aacgtatcta aaaaaataaa acggtttcta cggttttaaa tttagctatt
    21901 tttttaagtt atcttcataa tttcctcaat tatttagccc cagtcttcaa ctattcttca
    21961 tgaaatgctg ataaactaag aaaaagaata aaagaggtgg atcttatgca tttgattatg
    22021 attttcagtt tattaagtat tatcagttta tttgctttca gcgcttcccg cttattagat
    22081 gcggaaacac agcctattcc tgtaaaagta cgtaaaaacg accgctaaac ttccataacg
    22141 cgcgttctta tcctaaagtt ttttcagaaa cattgaaact ctctcccctc tctgataaaa
    22201 ttttaatttg tagcgaaaca aattaattac aaggagatcg gatatgtcca taaaaaaaac
    22261 acgtctagtc ctattattgg ctagtctcat tctttgcctt gcttcttttc taccagctgt
    22321 tgctgtgcaa gctgaagata cgttcaaagt aaatgcaaaa gcagcttttg ctgtagatgc
    22381 gcaaagcggt aagattcttt atgatcaaga tggagaaaaa accatgggga tcgcttcaat
    22441 cacaaaaatc atcggtcttt acatcgtcct tgatcaagta aaagaaggaa aactgtcatg
    22501 ggatgataaa gtttcgattt cagattatgc agaaaatttg agtatcaccc cagatttatc
    22561 gaatgttcct ttgcataaag aaaacaccta cactgtcaaa gaattattcg actctgcaat
    22621 catccagtca gccaatgctt caatggtggc cttggcagaa aaaatctctg gcagcgaagc
    22681 taaattcact gaacgaatga aagaacaatt gaaagattgg ggaatcaaag atgcaacaat
    22741 cgttaatgca tcaggattga acaactctta tcttggcgaa aaccgaccag aaggcactgg
    22801 agaaaacgac gaaaatcaaa tgtctgctca agatgtggcc atcgttgccc gccatttgat
    22861 cttggatttc ccagaaatac tggatgtatc aagtactacg acccaaatgt ttggcgaaaa
    22921 tacgcaatca ccagtggaaa tggtcaactg gaactggatg ctcccaggat tcgttaatta
    22981 caaggaaggc gtggacggat taaaaactgg tacgactgag cttgcaggtg cttgtttcgt
    23041 tggtacgatc actaaaaacg atcaacggat catcactgtc gtattgaatg cggacggcca
    23101 tgcggaaaac ccgagtgccc gcttcaatga aacagaccgt ttgatggact actgctatga
    23161 caattggtca gaaaaagagc taggaaaagc taacgcaagt attcctagtc taaaaaccat
    23221 tgatgtaaaa gacggaaaag aatcaacagt tcctgttgtg cttaaaagcc ctgtaaaagt
    23281 atgggtacga aacgatatgg atactggcaa attaacgatc acgccaacac ttgacaagaa
    23341 gcaagtcact gacaatgaga tccaagcacc tatggataaa ggtacaaaaa tcggtaccgc
    23401 tacgatcacc ttggcagacg ataaattagg ctatttagaa gacaacaatg caccttcaac
    23461 agagatcatt acggataaaa cagtggaaaa agcgaatata ttcgtgattg gctggcgacg
    23521 cgtcgcagaa tttttcggca acttgtttta attgaacaat aagaaaaaca tctttccatc
    23581 ggaaagatgt ttttttgctt tttctagttt aaataacagc ttttttcatc tattttcgtt
    23641 atactgtata agaaaaagga gttgaattta tgaatctaac cagagatctg atctttttag
    23701 ttctctatct tattggggta ttctctatcg tttattggcg aaaacagaag aaggatcctt
    23761 ccttgttctt ttacggcagt acgtggacat ttacggtaat cttaacttat tttgtcgtgt
    23821 tggaaatttt ttattcgacc ttttattttt tgattccatt gttgttcttt agtatctttg
    23881 ctttttctta tttcacagaa aaacgaaggc tgctgaatgg tcttttattc aatgttttcc
    23941 tcatcagttt cggcatctac ttgtttgtct tgctgtatga aacgcaaaat attttccttg
    24001 gcggactgat cgccttgatc acgattccat tgctacttgt ccttttattt ggtatttatg
    24061 gattgattgt ttttcttttt tggaacgggg tcacagtttt acgtagagaa tcgcattcat
    24121 tagctaatct tttaacgttg atactagcca tttttcttac actgtttctt gtttttgact
    24181 ttttcttgtt aaaatatttg ccgcaatgga taaatgcttt gtttttctgc gttcccctga
    24241 tcttgatcta tctatttatc gtattctaca actttttgac tgtctcattc ttgtatcagt
    24301 tcaaccggcc tcgatacaat caagacttca tcgttgtgtt aggcgctggg ttgatcaatg
    24361 gagaaacagt ttctcctctt ctagccaagc gaatcaataa ggcaattgct ttttatcggg
    24421 ctcaaagccg tgctacatta aatccgccca tcttattgat gtctggcggg caaggagcag
    24481 acgaaaaagt accagaggcc attgcgatga aacaatatgc catggaacaa ggaatccctg
    24541 aaagagacat tcttgtcgaa accaattcga caaccacttt ggagaacatg ctctattcaa
    24601 aggaaatcat ggatcagcaa atgaaagggc cataccgcgc gatctttagt tccaacaatt
    24661 accacatttt tagagccggt ctttatgcgt ggcaagccaa attgaaagca aatggtattg
    24721 gtgcaaaaac agctttctac tacttgccta atgcgttttt aagagaatac atcgccatct
    24781 tgatgatgaa caaaaaaaga catatgatca tttgcggatt gcttttcgca ggctccgcat
    24841 tcttatcgct gattacttta cttttctaaa aaaacaaggt gtgacaaaac atatgtcaca
    24901 ccttgttttt ttctttcatt ctcttaacta ttggcaaaaa gagtatccct tttttgaccg
    24961 ctacttgctg agcagaataa ataccgctgt tgcctgaaca cataaagctg atcaagcaga
    25021 tcataaagaa gtatactgcc gcttgtgaac cgaacaattc gatccccatc acaaagcagg
    25081 caatcggtgt atttgtcgca ccagaaaata ctccgataaa gcccaatcct gcaagaaaag
    25141 gaacagaaac atggagcaaa ggcgctagtg ctgcaccaag cgttgcacca atttcaaaca
    25201 atggtgtcac ttctccccct tgatatcccg cgcctaaaga caaaactgtg aagaataact
    25261 tcccaataaa atcatagaca tgtgcctgtc cattaaatga ttcttgaagt aacggaagac
    25321 tcaatccgag ataacgttgt gtttgcaaaa tcaatgctgc tgccacaact acagctccac
    25381 ctatcgtatt tcgccaaaca ggatgaggaa tccattgtgc atatcgcgct tttaaaaaga
    25441 cgatcgaacg gctgaatacc caaccaatca aaccaaaagc aatcccagca acaaaaactt
    25501 tgaagaacag caacatactc cattcaggaa tcacacccat ttggtaatgt agatgagaaa
    25561 caccatagct ttctgtcatg aaatttgcaa acagtgcagc aaagaagcta gggaagatcg
    25621 cttctgttcg gactttcccg attgccaaca cttctaatcc aaataatgtt ccagctaaag
    25681 gtgtgccaaa tacagaacta aaacccgcac tgattccact gataatcaag atttcccgtt
    25741 ctgatttatc caatcggaac aaacgtgctg tattatctgc taacgctccc cccatttgga
    25801 ctgctgttcc ttcacggcca acagatcccc caaataaatg ggtagttatg gttccaaaca
    25861 gtgtaagtgg aataagtcgc aacgggattt tttcttctcc accatttcct tgatcgatca
    25921 ctaagttgtt tccacgacta gaaagaccgc cgtgatacgc atagagataa gcaaaaagcg
    25981 caccactaac tggtagtaga taaaggagcc aaggatgtga tagtcggata tctgtcacaa
    26041 ctgtcaggct atttaagaaa aaagcagaca agctccccat gaatataccg agaatcgctg
    26101 tgatcaccag ccacttcagc atgtacacta aaatgcgtac tggactcggt aattgttttt
    26161 ccataaaaaa ttcctcctca ataaaaaaac gcctactaaa gaaaggggct gtccccagac
    26221 tttagtaggc gtcattagct atctttcgtt agcatttaaa ggcgaacacc atcacctatt
    26281 cacttccatt aaaagatagc atatttttct gtaaaaaaca ataaaattat cgttctttct
    26341 ttacacgttc agcaaattca tagaaaggag ccaatttcgt taactgttga ttacattggt
    26401 cactatcatg acaaatataa ttcccctttt tcgtatacgt gccatcagaa cctgctttcg
    26461 ttgtagaaag aaaaagcgaa acattcgatg ttttctgaca gattgcacag attcccttat
    26521 ttatcgttgg tgataaagta ccactaatcc cttttaattt ttcatcttcg taatagatca
    26581 tgaatttttt ctgcgtacct ggatcattcc agccgatgta cgtatgttct tgcaagtcga
    26641 tttcattcca agctggttgt ttgagttttt tcacttttcg gaataatttc tcgatttgtg
    26701 gtttgctagg ctgcataaac ggctgaacga tttgcttcag ctcctccaat gctcgttctg
    26761 cttgcgcttt tgtcagtttt acgtctaaaa ctgtcgttaa aaatgtatcg atttctggca
    26821 gttcgtgttc taatatgtct cgaatctttt cagttgctag tgcctgaact gtttgtaccg
    26881 tttttggatc attgacagat gagtacgcat ttaataactg ttctacttct ctttttataa
    26941 aaaaatattg gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca
    27001 aagtgcatac tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc
    27061 ggactctttt aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattct
    27121 tctatgtaca tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac
    27181 tgcaaacaga aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat
    27241 ggtgttcaaa agcttgcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagta
    27301 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg
    27361 gtctattatt ggttttacct gttaaatcga ggattctttc ccaaagtaac gttgccatct
    27421 cttacgcata cttgcagaca tgggctgagc cgcttctttg actgcgcagt atttgtccac
    27481 gaatgtcaca atatatcctt ctttatactt aggcggtgcg atcgttgcac cccacatgtg
    27541 cttcaaaatg atatctcgct caagatccga caaatcggtg attttttctg cattctttac
    27601 agcaattctt ggatggatat acgcatgtgt cccctcgtca aatttcgtcg tacgccaatc
    27661 atagtagaac agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca
    27721 tttttttgcg agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga
    27781 atgaacatgc tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttg
    27841 tacataagac atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc
    27901 acctttttga atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt
    27961 attctagtcg ctctagataa aatagctaaa gcttaatagt tccttaatca taaagaaaaa
    28021 tgataaagaa cgatccctgc tgcgactccg acattcaatg attccgcatt tcctttcatt
    28081 gggatatata cgttctgatc agtcaaagac aagatttcct gacttactcc ctgaccttcg
    28141 tttcccatga ttatagcgaa atcctctgtt ttttctactt tgtctaatgc atgtgcttct
    28201 tcatttaatt ctgtaccgta caccgtaaac cctttgcctt tcattacagg gatcacttct
    28261 gccaaatctt tttgtaacac aggcaaatga taattgcttc cctgcatgct tcgcagcacc
    28321 tttgtactat aaatatctgc cgttccttta cctaataaaa caccagccat accaaacgca
    28381 tctgccgtac ggatcatcgt tcccacattt ccaggatcct gtacattatc tagtaaaagc
    28441 cagcctcctg aaaaatctgg cgtatcttca gatgttaacg tcatcaccgc taagatccct
    28501 tgcggtgtag gtagatctga catagcggtc atgacctcat ctgaaactag aaaagtagaa
    28561 atctctttgt ttacagccag ccagtctccc cattctttca tacctctctg tgtcacaaaa
    28621 agttctttta tttctgcatc agacgacgca gcttcttcga tcagatggaa cccttccaaa
    28681 agatattctg ccttttcttc tctgtatttc tttttatgta gcttttttaa ttctttgatt
    28741 tttggatttt ttgctgattg gatctctttc acattccttc acctcacctg attatatcat
    28801 ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattgat ttataaaaca
    28861 ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaat gtccaaggac gtgtccaggg
    28921 cgtgggattt cgctatatga ccaagatggt tgctgatcag ttaggcgtga ccggaacggt
    28981 aaaaaacgaa gacgacggct cagtcacaat cgaagcaatc ggaaatgacg acatcatcca
    29041 aaaatttatc gaagaagtca agaagtctcc gagcccaagt ggacgagtgc agtatgtgga
    29101 tgtccaagaa gatccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg
    29161 tgaaaaggtc gcttagtttt gagtattaag gaaaattttg tgaaaatggc tttccacatt
    29221 tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgtcg atcaggttgt
    29281 ttttgaggcg ctttgcctca agtattaaag ataggtagta aaaaagctga tctataactt
    29341 gcaataaatt tgcttaaccc atattttcca gacagaacaa aaaagttttt agttttttat
    29401 ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc ttgatttttt
    29461 gtcttttttc aaaatttttc gctacgaaaa agaaagaata tttatatgaa atttctatga
    29521 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
    29581 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
    29641 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga
    29701 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
    29761 ttcaattggt cttacggctg ggcagttatt gcaatgactg tgatcgttcg tatcatcatc
    29821 ttgccactag gtatcagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
    29881 ttaaaaccac aagttgaagc agcacaacaa aagctaaaag ctgcaacaac acgtgaagaa
    29941 caaatggcag cacaagcaga aatgcagcaa gtatatcgcg aaaacggatt gagtatgacc
    30001 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt
    30061 acggctcgct acacagaagg tatccgtgaa tcatcttttt acggcatcga tctaggctca
    30121 ccaagtatgg tgcttgtggc cattgcaggg gtcgcttatc tgcttcaagg atatatttca
    30181 acaatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct
    30241 gctatgatcg tcttcatgtc gatcagtgcg ccagccggcg ttacacttta ctgggtagtc
    30301 ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgcttg
    30361 aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt cactccaaga
    30421 aaagacgtga cagaatcagc aaaaccagct gacaacaaac aagcaacaaa acaaatcaaa
    30481 caaccaaaaa acagtacaaa taacaaaggc cgcaacgcag gaaagcagca gaagcggaag
    30541 tagatagtaa ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg
    30601 accaagattt ttgaaaaaga aatctagtcg atatttttat ttaagtgaat gtataatttg
    30661 aatcatcgat tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt
    30721 cttatctatc aacgtagaca ggtgttgctg tttcttttaa tagtgtactt aattccttaa
    30781 tattttgttt tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa
    30841 aaggtgccac tcccttcgcc catgttgctc gcccacataa gacaccattg aatgtcgaac
    30901 ctgattgttt tgcaaaacgt aatgtatctt gaaataacgt agcattgact cccgcactta
    30961 aaaaaataaa cggttgttca gtcactttcg attgttcata gaaatacgcg gatgcctctg
    31021 cttgactata acaagtctct tgcccatatc cttccacaaa tctcatattc actggcactt
    31081 ctactttcaa tacatccact tgataacgtt cttttgaaaa ttctcgcatg gcttcaataa
    31141 ctttatgggg ttttactttt gcataggcaa gagaggacgg atcgccaata gttgcatcat
    31201 aagaaaggat ctctaaaaag aaaggtatgc cttctgcatc gcattctgaa ccaatccgtt
    31261 ctataactat ttgtttttgc acattaatct ctgctggttc atccacgtca tagtacaata
    31321 aaaatttaca agcatccgca ccagcttctt ttaacctttt caccgaccaa atattcaata
    31381 aatcaggtaa tcttcccggc attttcgtat catagccggt tttttcataa gctagcagca
    31441 agccacattc tttctctcta agcgctgctg caggcaggcc gcattctgga tcaagtaaaa
    31501 tagctgaggc atagggagtc aggtattgag aaaccatctg tttaaattgg atgatgtctt
    31561 ctgatgtagc ttctttatat tggttgatca ttttcttcaa agcaccacgc tggtcgattg
    31621 ctaacgcgct aataattcca gcttcatccg tcaatcgctt caaacgttcc tgcttttcta
    31681 tactcatctt cttcatagat cttcacctat ttctttagtt aatattgtgg ctatttctgt
    31741 ttttgatttt gcttttctca actgttctgt aacctctttt cgcatcaatg atcgtgcaac
    31801 gagagacaat aatttcaaat gagtcgttcc tgcttctgaa tctggaataa ataacgcaaa
    31861 aataaaatca actgactgtc tatctaaact gttccattca attcctttgt tcagtgtgac
    31921 gataatcata ctaggttcgt tgattgtttg agacttcgca tgagggatag caaaacctga
    31981 cattattcct gtagtacctt ctttttctct ttctagaagt tttcgatata cttctttttc
    32041 gtcatgagcg attcctaaac agaatgtttg ctttgctaaa aacttaaatg cttcttcttg
    32101 actatctaat ggctgttgta aaaaaatgtt ctcttcatga atgattgtca tgataagcct
    32161 cctgctcttc cttcttcgat aactgtacga tttgctgctt tattatccat cttcttgtct
    32221 ttatcagtta ctcttttctt ttggatcaaa cctagtatca ctccaccaac gatcgaacca
    32281 ataagaatcg ctgtaaccca ttggattttt cccgtcacta caggtaacac taaaaaaccg
    32341 ccatgtgggg ctggtacttg gacaccaaac agataggtca agacagcagc aattgatgag
    32401 cctaacatca tcgttggtaa tactcggatc gggtctttcg cagcaaacgg aatagctccc
    32461 tccgtaatat gagttgatcc taaaataaaa ttaaccaaac ctgcattacg atcattagta
    32521 tcaaaatatt taccaaacaa taaagttgca aaaccagtaa tcaacggtgg tgcaatgcaa
    32581 gctactgaca cgccagccat aaaagtcgtg tttccttgtg ccaaaagagc tgtacctgtt
    32641 acatatgccg ccttattaat cggtcctccc atatcaaacg cacacatgca accaacaatc
    32701 acgccaagaa caagtggatt cgaatgttca aatccagcta aaaagctcat cattccttca
    32761 tttatagcct tcattggacc cgccagcaaa gacatcacga tgccgataaa cactacacca
    32821 ataactggat ataagaagat tgccttcaag ccatctagtt gtttaggtaa aattgtcagt
    32881 agcttttgca aaaataagat aaaataacct gctaagaacc cagctaaaat acctactaaa
    32941 aatccagtac cgccactcat ggccacgatt ccaccaacaa atccaacgac taaccctgga
    33001 cgtttcgcga tcccttctgc aatataagct gctaaaactg gaaccatcat ccccattgct
    33061 gcatcgccaa ccgtttttaa catcgccgct gtagcattgt attgtgcatg gtctggatca
    33121 aaagaataga ttccccaaac ggcaaatgaa atagcaatca atactccgcc agctaccacg
    33181 aaaggcaaca tatgagagac gccattcatt aaatttttat agatgctgcg accaaaatta
    33241 gcaccactta tcgtttctga ttctaaagta tcaacagctt cattatctgc taagatggtc
    33301 ccttttcctg ccaatgcttc gtcaatcagc cgttgtgcat ccttgattcc tgtactgaca
    33361 gaaacatcaa tgactcgttt tcccgcaaat ctttctgaat ggacatcttt gtctgcggca
    33421 ataatgacag catctgcctg tttgatttct tcagccgtta attcattctc gatcccaatt
    33481 tgtccatggg tctccacttt gatcgtgatg cctttttttc tagctgcttg ctctaatgcc
    33541 tcttgcgcca tgtaggtgtg tgcaattcct gtcggacaac cggttgctgc aattagttga
    33601 tattttccca tctttcgaac ctctcttcct tgattttgat ttgtttactt aattcctcca
    33661 catccaaaaa atcagtcagt cctttacgaa aagcagtaga gctgcctgct gctatgcttt
    33721 tacgtaacgt ttcatcaagc ggtcgtcgat tcatgtatcc tactaaaaaa gtgcctaaca
    33781 tggcatcacc tgcacatgct gtattgacaa cttttccttt tggcgagttt cctcgaatca
    33841 ccctgtcttt cgtaaaaaga atcgctcctt tctcaccaag ggataacaaa acattttctg
    33901 cacctgactc taccagtcgt tgtccataaa caaagtaatc ttcatctgta accatttttc
    33961 gtccgaacca agaagaaagt tcttcctcat tcggtttcag caaaaatggt cgatagggca
    34021 aacaatccat gacctcttga tcactgctat caataatcaa atgaatctcc ttttcctgac
    34081 aaatccgact gatttccact aaaaccttcg gagacattcc tcttggtaaa ctcccagaaa
    34141 cacaaagata atcctctgct tgtaatgctc ttatttgatt taaaaaatta tggacagctg
    34201 cctgcggtat ttcaggtcct tgattcacta atttgtattc ttctctcgtt tgattgactt
    34261 gtgtaaatac attgatacgc gtcataccag gcacttcaat aaactctgtt tggatttgtt
    34321 tcttctgtaa atagtcttct atatattttc ctgtgaaacc agcagaaaaa cctaatgccg
    34381 tacttgggaa atctagcatc tttaatacta gtgacacatt tactcctttt ccgtttgcct
    34441 gaatgtcatc ctcctttgta cggtttacca taaaaggatg catctcctct gtttcaataa
    34501 atagatcaat tgccaaattc atcgtgcatg tgtagataga cataaccaat gacctccttg
    34561 aatctattat gaaatacgcg aaagagaaaa tgttttctta atcgttccaa cacaaagaaa
    34621 acctttacat ttataaacac atcgtgctat tttttaacta acttttagtg aaagaaggaa
    34681 agaatatgga tcacaaatta agtgaaacag aacagtacct ctggaacttt atcgaacacc
    34741 acatcctaga gatccctaac tactccatcg tgaaactaag tgaacaagca aatgtctcaa
    34801 cagccaccat tgtgcggaca atgaagaaaa aaggctatga aggatttact tcgtttaagc
    34861 accatctaaa agaaggagaa aacaagaaca tcaatttttc tttttagata aagtagacaa
    34921 aggtatccgt cgagccattc taaaaaatga acaagaagtg gtacgtacga tcaatatgtt
    34981 ggaaatcggg aacattgaag atgcaataca aagaatcaaa tttactgacc gggtttttgt
    35041 ctttgcccga ggattttcag aaatgattgg tcaagagatg ctcgtaaaac ttcaattaac
    35101 aggaaaaaat tgtcaaatgc atactgatcc agagattatc aaaagcatca gtcggaaact
    35161 aactaagaaa gacagcgtta tttttgtttc tttgaacggc gagacaaaag aactcgtcac
    35221 cgccgcaaag aactgctatg atgcagagat tggttcgatt ctagttacag ccaatcattc
    35281 ctctacactc aaagattatt gcgagataaa tctcgtaggt ttcaaatcag aaggttctta
    35341 tttccctgat tatgaagtac gttcacgctt gcctctccag atcatcgcga gaatcctgct
    35401 tgacgcctat gtattgcgta tgagggagaa ttgagaagca gtttattatg gttggttaca
    35461 gtttaaaaca tcacattttt tattctttat attgtatgga ctcacgtaaa ctttttatgt
    35521 ataaagttat tcacaatcta taaagttatt ttaagtaaca aaaaataatt gaaaatactt
    35581 aaaatcaaaa aatatctttt taatcaataa aaaaattgac agttttatta ataataagct
    35641 attctgttat agaagaaaaa attgtttgtt attattagca acaaaaaaag gagtgcatca
    35701 tgaaaaagtt atctcaaaaa cgtttagctc aaatcattat tgattctaga aaatctctga
    35761 aaatgacaca acaacaactt gcagtagcta ctggtatcaa ccgtgcctta attagtcgag
    35821 tagaatctct cgattttatc ccgtcaatac aacaattaga atcattagca gaagttctaa
    35881 attttgaacc aacagatcta tttataaatg aatcaaataa atcaaacaga acaaaacatc
    35941 agtcgaagaa tattgctgta gccggtactg gttacgtagg tctttccatt gctactcttc
    36001 ttgcccagca caatcaggta acagcagtag acattcttcc tgaaaaagta aaacaaatta
    36061 acaataagaa atctccaata caagacgatt acattgaaaa atatctagct gaaaaaaatc
    36121 ttaatttaat tgcaacgact gatggtgcag ccgcctatag agaagccgat tttgttgtca
    36181 ttgctgctcc aacaaattat gatagtaaac aaaattattt tgatacttct gctgtagagt
    36241 cagtaatcaa attggtgtta gaagtcaatc caaaggcaat aatggtcatc aagtcgacgg
    36301 tacccgtagg ctataccgaa agtattcgta aaaaatacaa tactaaaaat attctattca
    36361 gcccagaatt tctcagagaa tctaaggcat tatatgacaa tctatatcct tcgcgaatta
    36421 ttgtcggcac ggaccaaact gacaaaactt tacgagaagc ctctcacact tttgcagatt
    36481 tactccaaga aggagcaatc aaaaaagata tcgaaactct tttcatggga ttcacagaag
    36541 ctgaagcagt taaattattc gctaatactt accttgcact tcgagtatct tacttcaatg
    36601 agctggatac ttatgcagaa atgaaaggac taaatacaca agaaatcatt cacggggttt
    36661 gcttagatcc aagaattggc acacattata ataatccatc ttttggatat ggaggctatt
    36721 gtttaccgaa agatacaaaa caacttctca ctaactataa cgatgttcct cagaatatga
    36781 taagtgcaat tgtcgatagt aatcgtacaa gaaaagactt tatagcagat cgagtgttag
    36841 aacttgctgg cacgtatgag gctaacagtt cgtggaatgc agaaaaagaa aaagaggtag
    36901 tcataggtgt ataccgtctt acaatgaaga gtaattcaga caacttccgt cagtcatcta
    36961 ttcaaggagt tatgaagcga attaaagcaa agggggcaac tattgtaatt tatgaaccaa
    37021 cacttgaaaa taacactaca ttttttggtt caaaagtaat aaataattta gatgatttta
    37081 aaaatatcag tcacagtatt attgctaatc gttacgatag taatcttgat gatgtaaaag
    37141 aaaaagtata taccagagat ttatttcaac gagattaaaa aataacacct cagatttgta
    37201 gtgtctgagg tgtttctata aacttttata agtaaaaaaa tacaattata cttcataaaa
    37261 agacttatac cactgcgcaa atttacgaag cccgtcacgc aaacttgtac ttggtttaaa
    37321 ttcaaaatct cgttctaacg cactagtatc tgcataagta actggcacat ctcctggttg
    37381 cattggaact agttcggtat gagattcaaa gtcgtaatct tctggtagca cccctgctgc
    37441 aattagctct tgttgtagaa tatctacaaa atctagtagg ttttccggcg tattgttacc
    37501 aatattataa atatgatatg gtgggattgg tagcccatct tccccatcaa ttttctctgg
    37561 agcgcactgc ataaccctct ttactccttc aacaatatca tctatatagg taaagtcacg
    37621 cttgcaattc ccataattga atatttggat attatcacca gcacgaagtt tatcagtaaa
    37681 accaaaataa gccatatctg gacgaccagc aggaccatat actgtaaaga aacgtagccc
    37741 tgtagacgga atattgtaaa gtttagaata tgcatgtgcc attgattcat tacttttctt
    37801 agttgctgca tacaacgata cttgattgtc tactttatcc tctgtagaat aaggaatttt
    37861 cttattagta ccataaacgg aagacgatga ggcatacact aaatgttcca caccagattc
    37921 tccattatca tatgaatgac gacaagcttc aagaatatta tagaatccta ttatgttaga
    37981 ttcgatatac atatctggat tagtaattga ataacggact cctgcctgag ctgctaaatt
    38041 caccacgata cttggtttat aattatcaaa gaggaaatca attgttgctt tatcagcaat
    38101 agaacctttg ataaaattcc aagtagatcc tggatggttc tttgcctccg tttctatctc
    38161 tgataatcta tagttcttaa tagagacatc atagtaatca ttcatattgt caatgccaat
    38221 gatttttatt gatgactgag tacgtaataa ctcaagaacc aaatttgatc caataaaacc
    38281 ggctgcgcca gttattaata ttgttttatt ctgtaaagat gtattt
//
LOCUS       DACDVT010000021        37719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDVT010000021 DACDVT010000000
VERSION     DACDVT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.021"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            215..483
                     /locus_tag="IWL52_001081"
                     /pseudo
     CDS             215..483
                     /locus_tag="IWL52_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1155..4556
                     /locus_tag="IWL52_001082"
     CDS             1155..4556
                     /locus_tag="IWL52_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9777658.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(4980..5150)
                     /locus_tag="IWL52_001083"
     CDS             complement(4980..5150)
                     /locus_tag="IWL52_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9777659.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5344..5523)
                     /locus_tag="IWL52_001084"
     CDS             complement(5344..5523)
                     /locus_tag="IWL52_001084"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777660.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5910..7022
                     /locus_tag="IWL52_001085"
     CDS             5910..7022
                     /locus_tag="IWL52_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9777661.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(7100..7393)
                     /locus_tag="IWL52_001086"
     CDS             complement(7100..7393)
                     /locus_tag="IWL52_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9777662.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7561..7875
                     /locus_tag="IWL52_001087"
     CDS             7561..7875
                     /locus_tag="IWL52_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9777663.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7896..9875
                     /locus_tag="IWL52_001088"
     CDS             7896..9875
                     /locus_tag="IWL52_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9777664.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9888..10214
                     /locus_tag="IWL52_001089"
     CDS             9888..10214
                     /locus_tag="IWL52_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9777665.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10230..10748
                     /locus_tag="IWL52_001090"
     CDS             10230..10748
                     /locus_tag="IWL52_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777666.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10748..12100
                     /gene="celB"
                     /locus_tag="IWL52_001091"
     CDS             10748..12100
                     /gene="celB"
                     /locus_tag="IWL52_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9777667.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12184..13689)
                     /locus_tag="IWL52_001092"
     CDS             complement(12184..13689)
                     /locus_tag="IWL52_001092"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9777668.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13781..14371)
                     /locus_tag="IWL52_001093"
     CDS             complement(13781..14371)
                     /locus_tag="IWL52_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9777669.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14550..16196
                     /locus_tag="IWL52_001094"
     CDS             14550..16196
                     /locus_tag="IWL52_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9777670.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16245..16964
                     /locus_tag="IWL52_001095"
     CDS             16245..16964
                     /locus_tag="IWL52_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9777671.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17563..18510
                     /locus_tag="IWL52_001096"
     CDS             17563..18510
                     /locus_tag="IWL52_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9777672.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18522..19442
                     /locus_tag="IWL52_001097"
     CDS             18522..19442
                     /locus_tag="IWL52_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9777673.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19483..20940
                     /locus_tag="IWL52_001098"
     CDS             19483..20940
                     /locus_tag="IWL52_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9777674.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21285..21899
                     /locus_tag="IWL52_001099"
     CDS             21285..21899
                     /locus_tag="IWL52_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9777675.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21892..23616
                     /locus_tag="IWL52_001100"
     CDS             21892..23616
                     /locus_tag="IWL52_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9777676.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23613..25064
                     /locus_tag="IWL52_001101"
     CDS             23613..25064
                     /locus_tag="IWL52_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9777677.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25537..27690)
                     /locus_tag="IWL52_001102"
     CDS             complement(25537..27690)
                     /locus_tag="IWL52_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9777678.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27692..28738)
                     /locus_tag="IWL52_001103"
     CDS             complement(27692..28738)
                     /locus_tag="IWL52_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9777679.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28888..30174
                     /locus_tag="IWL52_001104"
     CDS             28888..30174
                     /locus_tag="IWL52_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9777680.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            30190..32892
                     /locus_tag="IWL52_001105"
     CDS             30190..32892
                     /locus_tag="IWL52_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9777681.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32877..33734
                     /locus_tag="IWL52_001106"
     CDS             32877..33734
                     /locus_tag="IWL52_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9777682.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33805..34662)
                     /locus_tag="IWL52_001107"
     CDS             complement(33805..34662)
                     /locus_tag="IWL52_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9777683.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            34873..37044
                     /locus_tag="IWL52_001108"
     CDS             34873..37044
                     /locus_tag="IWL52_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9777684.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            37112..37453
                     /locus_tag="IWL52_001109"
     CDS             37112..37453
                     /locus_tag="IWL52_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9777685.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT        12333 a         6550 c         7822 g        11014 t
ORIGIN      
        1 ttctggaatt tgctgtgtta aaaagcagta tgactaatat aaatactatt gtcatttatg
       61 caatttttta tggatagtaa ttttagaggg gaatcaatga aggaagaata gaagggaaaa
      121 atagttgcaa atacgccagt tagcaaaaat agcaatacat cttcaagctt taaaattcga
      181 gagaatcatc ttggtagtgg aatcaactac aacaaaagga ttaaaccaat taatagatag
      241 aattaccaat ggaaaaatag aaaaggtagt tattctatac aaaagttgtt taattcgctt
      301 cggttatgaa cttagtgaaa ccctttgcaa tatatggtac agaatataga aattattgac
      361 attacagaaa atgatgaaga tttaatacag attgtcacta tttgtagata ccttttacaa
      421 ggaaaccgag ccaataaagc taagaagatg attaaggagt tgatggaaga cgatacaagt
      481 taagaaagtg aaactacgcc caaataaaga gcaaaaagag taattggacg tggaaaatgc
      541 aagaaatgaa ccatcgattc ggtggacagt ttattagtaa tgacggtttg agaacatcca
      601 tgacgagaat gaaggaacat cctaaatgcg tttagttgaa ccaagtgcca aataatatgg
      661 ccaaataagc gatcagttat cgatgaatcc caagtattct aaactatata ttcattttga
      721 tgctaagcat tgatatattt ttttgggatt gatgaaggtt aaccaataca gggactcact
      781 gatgaaattc tagagtctga ccttggtatc aacgatttag tgattgtttc taatggaaaa
      841 gtataaaagt atataagtac attaacaaaa taactattag ggttacgctc cagcttgtgc
      901 aaggcactgg caggaggaat cgtgaaatca taattatggt taagccacac ggacaactaa
      961 aagtcataca aagctatcga atcaatcaac agaagacaat cggcaatcat tagctttgtt
     1021 tacagcttac attgcaacgc ttgagttgaa ccgaaaatac gagggaggta acgcagccat
     1081 tttgagttgg ggaatctttt tagaaaatta tcgggtacct tgttgtttag tcaaaattat
     1141 aagggggagt aatagtgagg aacaaatttc ataaactgtt aagtatgttt ctagttatgc
     1201 ttattgtgat caatcaattt gggaacataa cggttctagc tgagtcttta gcagaacagc
     1261 cagagcgttc gctgacgatc gaagttgatc aagaggaggc tgctttgggt gagcagttcc
     1321 agttgcgagt cagaggaaca ccagacctgc taaatgcatt ggtcattgat aagagtgagg
     1381 atttgattga aggggaagtg aagcaactca atgattacga acgtctgatt acttattctt
     1441 cccagcattc aggtaaatac gaattgaccg caagaagcgg tgatattcaa tcaaatacgg
     1501 tctcaataga aatcagtgta ccagaagaaa gcacaagtga gtcttttaat gtaccagaag
     1561 aaagcacaag tgaatctttt aatataccag aagaaagctt aagtgaatct tttaatgaag
     1621 ccagatcatc tgagccatct ataacgcagg aatctcagat tgaaagacaa gaggagctct
     1681 tgaattatga catcggcatc tctgctgagc gccccacttt gccgagcagg catacacaaa
     1741 cgttaaaggg atacattcaa ggaggcgctg gcgatattct tgaagaatat ggggagatgg
     1801 tcgtacgagt tcctagagca tcgattggct taattgagtt cattagagtg gaggtcgctg
     1861 atgattcacc tttaaagcca ctggcatttt accaagaagg cacagacgtt gtttttccat
     1921 ttacctttga ttatgaagga ttaaatgaag ccgctcagtc gtatacattc agtatcacgt
     1981 ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa taatgacttt caagtgagtc
     2041 ttacttatgc tgagcgagca gcaaacacgg aattttcaat tacgcctatt ccttcatcaa
     2101 gacccatttt ttcgaaatta tactatggct ctagagaaat cgacagccaa ggaaatcttg
     2161 ggaatctgct gattgacggg caagacgtga ccaacagtaa taaatttatt ttgcaggtaa
     2221 attatgaagg atcacgaaaa gacggtgtag tggttaccga tgtactaccg caaggaacaa
     2281 ctttggcacc gaatccgact acatggagtt ccactggaaa tagagaacct atttcaggtg
     2341 gggttcggat cgcagaagta acgaatccag gtgaagcaaa cgttagaact cattatgttt
     2401 ccgaggaatt tgtggacaaa gtatctttta ataaggaaac gaatacactt gttgtggact
     2461 ttggtaatct agcagagggt caatcgtatt ttattgaata tgctcttgaa gtgaccgatg
     2521 tcgacttgtt caatgaagga agacaagagg atgggttttc tcggaatcga gcgagtatga
     2581 cctacaataa tcaggtaatg agccgagact accgcttgag aatccctgaa agtaatcttc
     2641 cggcaatctc tttaagaaaa agtgtggaca aaaatgttat caatctcaat gaaaatgaac
     2701 taacgtatac tctgcatgtc tcgatgtttt ctggaacaag aaatacgctt gttattgaag
     2761 atgttttgga agaaggcatg gaatttgtgt catttctatc agatattccc tctttcattg
     2821 atgccgaagt caataatgga atagttcgtt tcagtgcgaa taatacgatt ggtggtccga
     2881 caagttttac cctctctttt aaggtcaatg tagaggcgta tgcggtcggc gatcaaattt
     2941 taaactttgc aacgcttgtg aatagtgaca atcgattgaa tacgagtact gtaagaacaa
     3001 ggaaaatcga tgggcgtatt aaagtaatta aaactgatga gaatggccaa aggctggcag
     3061 gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat acaagaaggt ataacggcag
     3121 aaaacggaga atttttaagt gaaccgcttc ctgtaggtag ctatcaagtc cgagaaacag
     3181 aagcaccgga agggtatctt ttagatgcga cggtacatga tgtttttata aatgaatact
     3241 ccatagaaga agtgacgtta accattgaaa acatccttga tacgggtggt gttgaactaa
     3301 ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc tgtttttgag ttacagaacc
     3361 gagaaggaga aacgctacaa accggtttaa ctacaggaga agatggaaaa ctggcaattg
     3421 acggtttagc accaggagca tatcagctgg tagaaactca agcgccaata gggtatgaat
     3481 tagatgcgac accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga
     3541 cgaaagaaaa ccgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg
     3601 gggaaatcct gcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa
     3661 ccggtctaac tacaggagaa gatggaaaac tggcaattga cggtttagca ccaggagcat
     3721 atcagctggt agaaactcaa gcgccaacgg ggtatgaatt agatgcgaca ccgatcgagt
     3781 ttgagatcga aagaagtcag acagcggtgg tagaattgac gaaagcaaac cgacttacac
     3841 caggcggtgt cgtgttgaca aagatcgatg accaaagcgg ggaagtcctg caaggagctg
     3901 tttttgagtt acaagatgcc aatggaaccg ttctgcagtc aggtctaacc acaggagcag
     3961 atggaaaact ggcgattgac ggcttagcac caggagtgta tcagctggta gaaacccaag
     4021 cgccaacggg gtatgaattg gatgcgacac caatcgagtt tgagatcgaa agaagtcaga
     4081 cagcggtggt agaattgacg aaagaaaatc gacttacacc aggcggtgtc gtgttgacaa
     4141 agatcgatga ccaaagcgga gaagtcctgc aaggagctgt ttttgagtta caagatgcca
     4201 atggaaccgt tctgcagtca ggtctaacca caggagcaga tggaaaactg gcgatagatg
     4261 gcttagcacc aggagcatat cagctggtag aaactcaagc gccaacgggg tatgaattgg
     4321 atgcgacacc agtaactttc aagatcgaaa aagaacaaga agtggcgatc tttttaacaa
     4381 aagagaatag aaaagttgct gatagctcag atataagagg aaacaatcca acaagcagtg
     4441 gaaataagca tcttccaaaa actggagaaa ccatcattag ccagtttata ttgtcaatat
     4501 tgggtgtgct acttgttttc atcagtataa aatttagaaa gaaacgaaat atatgagcta
     4561 aaataaaaaa ttgaataagc atagttttta aaatcaacta gtacccatca attgaatgga
     4621 aactaccgat tttagaaggg gtatacaaac aaatattagt atcggagtag agaaggttga
     4681 acccaaccag taaataagta aagacaattt tgtgctgcgt tgagaagtga aaaataaacc
     4741 tttgtagtaa tactagttaa taaaaaatat acaaaaacac tgcgaaaatc attcttgagt
     4801 ttcgcagtgt tttttgccta atgactcaag tttattgggt aatactttga ttttctttct
     4861 acaaataatc agcgatcagt tgtaattttt tctctagttt taatggctct ttgtcaataa
     4921 ggagaaaaac aagtcgggtc ttttgtctcg gaaagcttca accaactcgt gaataaaggt
     4981 tacgcctcct tgaaaggcgt ggaaaaacca agtaatcgat caatcaccaa tttcgcattt
     5041 ggtagaacat gtttggtgag ctgaaagtag gtggcgttca tgtctgtaac taagaattct
     5101 atttctttag gattagtgca gctaaggaaa tagtttgtta atcgaggaat tttttgtgta
     5161 ggtaaatttt ctcgattaat ttttctgtct cgccatccgc ggaaataaaa ctcagtttat
     5221 cctcaacgga aacgtgagaa cgaaattcac aaccctcaac actcaagaca gaattctttt
     5281 ggaagacttt ggtaaatagc ttctcaactc tttcaatgtg cgaataacgg ttgtcaaaga
     5341 tacctaacaa cttttcgaaa taaaaaataa cgacactttt tcagtcagta aagaagtgat
     5401 tttatgtctg acatgatttg aaatcgagtg ccttggacgg ataaaataac tttgagcgtt
     5461 ccaatggctt cgacagtttt tacaggtata gcgctgcttt tttaggcgta taaccattgg
     5521 catatgatta tattattcaa agtggacaat ggtttccttc ttcttgtttt tcaccaccat
     5581 taatttccta tttccatcta ccacagcaga accacaattt ctacagacac atggagcagg
     5641 gaagagaact gcagcaacga tcaatgtttt tttcttctgt aaggtttcgt aagagacctc
     5701 tgtaatcatc aaatttttgt ctattgatct cagaattttt ttgatagaac cattcataaa
     5761 gcatatcgtt ctctcagttg tttattttgt ggtgatttaa tcatacaaga aaacgatatg
     5821 tttttttata aaaacatgaa taggaaaaat agttgacaag aaaatgacaa cgccatatga
     5881 tgttccatag tatttacaac ctgtcataga tgactgtaaa aaataaaagt aaaaagaaag
     5941 gacgacaaca ggtggatttt tatcagtctc tacaactaga tccgtttatt ttaaaacaaa
     6001 aaatacgaga agcagcttct aaaaaagaaa agtgcatgta tatttgttca ttgtttttac
     6061 gatcattatt catcgtattg tttgccattt gttttatcat tataattaca acattatttg
     6121 aatcgaagca taaaccatac gctgtggttt tattctgtat gttgatgagt attcgttttg
     6181 ttgactttgg ttataagatt tcccattcga tcataggttt agcaatcgtc atgttctcac
     6241 tattagtagc accgtatgtc cagctgatca aatggtcagt tatgggcatg ttgattcatt
     6301 ttattttact aagcagcata ttaatggcaa ccgctagtga tcccaaaatg gggaatgctt
     6361 ctttgtacgg cttttcatat ctctttattg tttattcgtt acctaaagat tcgttgaaca
     6421 agaatttctt tacacaaaca agtagcttat tatttttgtt tttttgttgg ttttcagttc
     6481 tcctttacag aaaacatcga gaaaagaaca aagacaaaag cttgtttaaa gagatttttt
     6541 tgaaaggcat gtattcgcag gaaaaaattt ggatgctcat ctatgcattt ggcattagtc
     6601 ttttgatcgt tgcaggagag tatgtccctt ttcagcgatt gatgtgggca gggtttgcat
     6661 tttcttctat tgtatctagc tatggattga tgtcgattgg tttcaaagaa cgagcagtag
     6721 accgaatcat tggctcattg attggatgtg ttttatttat tggtatcagc caatttattc
     6781 cttttaactg ggtcggtata ttaggtggtc tagctttagg tgtttgttct acctatagat
     6841 ataaaacagt ttttaattct tttggtgctt tggcaatcac tgccagttta ttcggggttc
     6901 caggagcagt aacgatccgc atttttgaga atatccttgg ggtatgttta ggaatcatgt
     6961 atattggggt gactgaaata cttatccgaa aaataagaga aaaacacggt ttgaatcatt
     7021 agatgagatc gaataaaaaa taaggaaaaa gtgaagatac acatggatga taaattgcta
     7081 tcattccatg tgtatctttt tacggtaaat cagtgaaacg ttcgactttc atttttaatt
     7141 tatacttttt acggagttcg tttatttgag ccatcatttc ggatttgtga tgcttttcga
     7201 ttgcttcttc atctttccaa cggtcgatca gtaaaagaga ttctggatct tctagtggaa
     7261 agtaatagtc atatttctcg tttccttctt ctgcacgaat cgctttgaca atgtttcttt
     7321 cgaccatttc ttctgcaaat tttctggctt gtccatttgt acttgtatac aaaatattca
     7381 ctgtgatact cactaaatcc cctcctgaaa aaattctatc actagtatat cacggtgcca
     7441 gaaattatta ttcaagatat gatacacatt ttttccgcaa tgctacggaa atatatattt
     7501 tgaatagaaa acgcttacaa gatatgcttg gtttataaaa ataaaaggag tgattgattt
     7561 atgaaaaaag cattgattat ttgtgcggca ggaatgtcat cctcgatgat ggcctcaaag
     7621 acaacggatt tctttaagca aaaaagagaa gaggtactcg tagatgcagt ttcagctaca
     7681 gaaggcgata atatgattaa gaatagtgat tttgatttat ttctgatcag cccgcaaacg
     7741 acgatgtttt tggataaatt caaaaaatta ggagaatctg ttggaaagcc tgttgttagt
     7801 atccctttcc aagcgtatgt cccaatccct acaggcatcc agaagttagc agaggtgatc
     7861 gaagagaaca tatgatggaa taggagtgga tgataatgaa acagaaggaa aaattgctgt
     7921 tgagatattt gattgatcac aagaaggagt atgtcaccag tcaacgttta gcttctgaat
     7981 tgttattatc tgatcggaca atccgtaatt acttgcagcg gatcaaagag ttagtagaaa
     8041 aaaacggagg taagatcatt gctaaaccag gttatggcta ccaactgcat attttacaaa
     8101 gactgacatt tgacttgttt ttgagtcggc aagaaatagt acctggatat gctagagaac
     8161 cgcaaggatt ttatgagtcc gaagatcgac aaaagtatat attgaaccaa ttgcttttgg
     8221 aagatgccat tctttttatg gatgatttgg cagaagagct ttatatcagt cgttccagtt
     8281 tgacaaagga catgcaagaa atcaaggagc gattgattcc atattcacta aaaatcgttt
     8341 ctaagcatgg tcaagggaca tggatcgaag gggaagaaag aaatcgacgg cattttatca
     8401 tggatacttt ttttggaaac cattatggaa attcattgaa agaatattta gggcaaagcc
     8461 gttttttccc agacatcagt tttgaagagc tagtcattat cattctcgat gaaacaagag
     8521 aagctaagtt aaaagtttcg gattttatca tacagaatct gactttacac ttggctttgg
     8581 ggatcaaacg gctccgagaa gggtttgaaa tcaaggaatt aggcatcgaa caagaagttt
     8641 ctaagcgagt agagtatcaa gtggcgcagc ggatcgttca acgtattgag gtaatcgcag
     8701 gtgtatcgtt tcccgaagaa gaaattgctt atttgacatt acatctcatg gctaagtcga
     8761 atcacaaaga aaatcaaaat gatcaggaac tatcaaagga attagcgaaa gttctgaaaa
     8821 gattgtctca ggaatttcat ttttcattag tagaggatct ccagcttttc aatggactac
     8881 tcgatcattt gtcaccgatg atgattcgat tgagccgagg tattcgcatt gagaacccat
     8941 tgactgagga gataaagaaa gaaaatccca aagtctttga cgcagtaaaa aggcatttca
     9001 gcaacatgcc ggctttgaaa aactatacca tcaatgaaga tgaatgggcc tatttggctt
     9061 tgcatctcat ggcggcattg gaaaaagaaa gagctgcgca taaactacat gcattgatta
     9121 tctgtgcaac gggttacggc agtgcccaac tattaaagaa tcgcgtagtc agtgaattcg
     9181 gtaaaaatat tacagtggta agtgtaaaag gctattatga gatcaatgaa acaactttaa
     9241 aaggaatcga cctgattatt tcttctattg atctttcgac aatgtttttc aaaattccag
     9301 ttttacatgt cagtattttt ttgaatgaag atgatgttcg taaaatacga aaggtagttg
     9361 gagaatctat accctatcat tcctcagata gaccgatacc agcattttct ctacagcaaa
     9421 aaaagcagat ttacaccgaa cagttgtctg aaaaattttt caagacttat acgtacgaac
     9481 cgaaaaagga agaaattctt catgatttgc tgcaaaagct gagcataaat gaagaagaag
     9541 gatatataag agaaatgcga aagcagatcc agcaacgtga aaatatggga caaatcatat
     9601 tcagtgatac agtggcagtc ccacatcccg ctgttccagt tggaatcgaa acaaaggtag
     9661 ccgttgcatt gattccttct gggatgcagt gggatcaata tcaagaaatt cattttgttt
     9721 ttttagtttc gccttcttat gtcgaaaatg aaggaattac ggtcgtgact aaagcaatcg
     9781 ttaggttagt tgaccagcta aaagtacagc aggaaatctt agctgaacct atttttgaga
     9841 atttcagcag taaatttatc aaactgatcg aataaggagg aaaacagatg gctgaaaaaa
     9901 taaacagtga aacactacaa gtgacttctt ttgatatcat tttccatagc ggaaatgcac
     9961 ggacactgat acatgaagca tttcgctcga tgcgtaaaga gatgtttgaa gaagtagaag
    10021 agaagttgcg acaagcaaat gaagagattc ttcatgcgca tcagtcacaa actcaacttc
    10081 tgaaagaata tgccagcggg acaaaaatag aaatggaaat tatcatggtc catgcacaag
    10141 atcatctgat gacgacgatg acattattgg aagttgcaca ggaaatgaat tatttgtatc
    10201 aaaaaaataa atagaaagga aggatcattg tgcaggatac agtaaaaagg aaaatcgaat
    10261 tagaaaaaaa gcaacttagt ttgaaaaaca tgtatttcaa tcgttatcta atcgtacgtt
    10321 acctgactgc ctttttcttt ttcatgaaca tgcaatggat gattctgctt ctatcagcta
    10381 aaagtttagg aagtagttta ccgatgattc tgattttagc tattttgcct gcagtaggag
    10441 aacaagtaaa gctttatcga aaacaccaaa cgaacgttcc atggacaaag tggtattttt
    10501 tgttccagag tgtctttaat ttattagtga ttccagtgtt gtttacctca ggatttacat
    10561 tcttgtatcc ttttatggca aataacaatg gaggacagtt atttgttttc attcttgttg
    10621 taagcggtat ttttgccagt gcatttatac agtatcgttt aaagaaaatt tcactcaacc
    10681 aagaccaaca gtatatccgt attaaacaat atgaaaaagc gttatattta ggaaaggaga
    10741 ataactaatg tttgattttt tgcagaaata cctgatggga cctatgggga aaattgcgca
    10801 acttaaaatc gttcgggctg tcatggcagc ggggatggct tctattcctt tcacgattgt
    10861 aggctcgatg tttttggttt taaatatttt gccattaccg ttcccatttt tagaaggatt
    10921 ttttaatgca acttttttca aagttagtga cttgtacatg atcgtcaata caatgacgat
    10981 ggggatttta tctatttact ttgccattgt gtttgcttat gaattaacgt caattgaacg
    11041 agatgaacaa aaattaaatg ttaatccgct cacgggggca ttactatctg tttttgcatt
    11101 ctttatgtgt atccctgaat tgatcatgac tgatggtaaa atcagcttga ttgcttctat
    11161 caatgagaca gaaactgtag taaatggtat taggatgggc tcttttgtcg aacgtttagg
    11221 aacttcagga atctttacag ctattattat gtcgattatt gcggtcgaat tgtattgtat
    11281 gtgtgtcaag agaaattggg tcataaaaat gccagatgtt gttccgccag gagtttctcg
    11341 ttctttcact gctctgatcc caacatttgt cattgcgttt gtggtgatga tcatcaatgg
    11401 attattagtt gcactaggaa cggatatctt caaaatgatt gctatcccat ttgggtttgt
    11461 tacgcagttg acgaatacat ggcttggtgt catggtgatt tatttcttga tccatgcatt
    11521 gtggatcgta gggatccatg gagcgaatat catcacttct ttcttgaccc cgatcgtatt
    11581 agctaatatg gcagctaacg cacaaggcgc aaattatccg ttggcaggag aattcaacaa
    11641 ttcttatgta accgttggtg gatcaggtgc gactttagga ttgatcatct ttattgcatt
    11701 cctcgcgaaa tctgatcagc tgaaagtgtt aggaaaagcc tcacttgtac caggattatt
    11761 caatatcaat gaaccaatca ttttcgggat gccaatcgtc tacaatccct atttagcggt
    11821 tccgtttttc ttagctccga tggcttctgc tacactcgct tattttgcca ttcggctgga
    11881 aatcgtcaaa ccgatgcttg cacagatgcc gtggccttca ccagtcggga tcggagcatt
    11941 tgttggtagt ggtggtgatt ggaaagctgc tgtgttagca gtcatttgtg caatcatcgc
    12001 attcattatc tggttcccat ttatcaaatt ctatgataat aaattattaa atgaagaaag
    12061 agaaaaggca gcagaattag ctagtgaaaa gttaaaatga tttctaagaa aaaaggaact
    12121 gtgacaaaaa atcacagtcc ctttttttct tagaaatggg aaaccactag tatataaata
    12181 ccattattct tcaatcaacc gcttcacgat tgcataatcc ccttcataag gttcatctac
    12241 accattgatt ttttgatagg gatccacgcc ttttcccgca ataacgactg aatcttgcgg
    12301 agcgctttct gctaaagcgc gtcgaatcgc ttcaggacga tccatttcat aatgaacgat
    12361 caaatctgga ttagtaattg cttcattgat ttcttccgcg atttttttcg gatcttcaaa
    12421 tgctgggtca tctgccgtca aaaaggcgat gtcagctgtt tctgatagca cttttccaaa
    12481 atcatgtcga cgggagattg ctttgtttcc tgggctgcct aatacaacaa ttacacggcc
    12541 atctggatgt tcatttttgg caaatttcag caatgttttc aagcttagat aattatgtgc
    12601 atagtccaca tagacatgcg ctccattgga ttggatcaat tgatccattc ggcctggtac
    12661 acgagcttct tttaatccgt cttgtgcatc ttgactggag gcacctgtta gtgctgcggc
    12721 aatgatcgca ctggctgcat tttcttggtt gaatcctcca gccagcaaaa tatcataagc
    12781 agtatcgcag atagctaagc gatcttcatg agaagataaa gtaaaaccgt gagtaccttt
    12841 atctgaacgg atcacttgat aattgctttc ttccgaacga ccataagtga tcgttggaat
    12901 ctggaataga tcggctgttt ccctaagtaa atcaaaatag tcactttcat gatttaaaac
    12961 aactgttttt gaattcaaga tcaattgtcg cttgcaatag aaatagtcat caaatgtcgg
    13021 atgttcgatc ggactgatat ggtcaggcga gatattcaag aagattccga catctaaagt
    13081 caagccgtat acgcgttgtg ttttgtaagc ttgtgaagaa acttccatca ctagatgagt
    13141 cataccattt tcaactgctt ctgacatcat acgatataaa tctaatgact caggcgttgt
    13201 caaatgggat ttgaaatatg ttttgccatc taacgttgta ttcatagtag aaagcaatgc
    13261 tgttttttta tttgttgtgt gatccaagat tgcttttgta aagtacgcag cagttgtttt
    13321 accttttgtt ccagtgaatc cgatcacttt caatttattt tgcggatagt cgtaaaaagc
    13381 catagcaaga agcgccattg ctttacggat atccgttacg atgattccta ctgctgaatt
    13441 ttcgtatggt tgttctgata cataatattg aataccctgt tcgattgctg aatccaaata
    13501 ttcttctttg aaattcattc ctttacagaa aaacaaagta gaagcatcag cttttcttga
    13561 atcataactt aactgaagaa attcagcttt ttctggtaca tcaagatgcc agcctcttgt
    13621 tgaaacaaat tcttttaata gattttcttt taatagtaaa tgacggatgt cttctaaaga
    13681 gaagctcatt gattgatccc tcgctttatt caatttcttg gtttattata gcacactctt
    13741 tttaatatat cgtttgtttc cgctatgaca aaatccagct ttattcaaat tttcttgaca
    13801 caggggacaa gcggatgagt attttcataa acaatgaact gactgtaata gataggagaa
    13861 cgaatagtgt ctccaacaat ccaaaaacat aataattcaa aaagacgata agactattgg
    13921 taagtaaaag tccttgcggc attggaagcc gaaaaaattt gtggaaaata atcggcggta
    13981 tcgttatacc gccatttgag gccccgactg tgtacaatgt gtaaacccca agactgaaca
    14041 aaagacttcc tgaaatcagt gaaaagaaat gtgaggagat catttccact tttggaacaa
    14101 tctctaaaac aacaggaaaa agcagacttc ccgctacagt cttaaaaaat actgattttc
    14161 ctaataggac agcggctaga agcagcatca taagattgat gctccaaaca gtaactgagc
    14221 gttggataga aaaggccgtt tcaactaaat gtccgatggc gcccactccg gcggaagcta
    14281 tatcgtgagg gagcaagaac caattcaatc caactgtgat aaagaaaaga cctaaaaata
    14341 taacgggtag tttttttatt tcttgtgtca ttgatatctc ctataaatgt tagccataaa
    14401 aggctgtata tttctttact ttaccaaaaa aacgaaaccg aattcaaaaa aacaattgtt
    14461 cttataaaag ttatataaaa tcgattgcca gtttctgttt taccaattaa aatgatagta
    14521 atgttttgta gaaagttgga atctgaccga tggataatca aagaaaacca gcattagatc
    14581 aattaacacc gcaagaaaaa atggcacgag gctctgcttg gatgaccgcc agtaatatca
    14641 tttcaagatt gctaggcgct gtctatatca ttccctggta tgcttggatg ggcgaaaatg
    14701 ctaaggctgc caatggatta ttcaatatgg gatacaatat atatgcattg tttctactga
    14761 tctctacagc aggaatacct gctgcgattg caaaacaaac tgcacgctat aattcattga
    14821 atgaatatgg cacttcacga cgattattta tccgtgcatt acagatgatg ggcgggttag
    14881 gcctgttgtt tgctttgttc atgtacatcg cctctccttg gctagctcat gcttctggtg
    14941 gcggagaaga actgattcca acgatgcgtt ctttgagtat cgctgtattg gtttttcctt
    15001 gtatgagtgt gatacgaggg tattttcaag gcaatcaaga aatgatgcct tatgcgctgt
    15061 cgcagattgt agaacaggtg gcacgtattt tttatatgct attggctaca ttcatcatca
    15121 tgaaagtcat ggatggtgac tatgtgacgg cggtcacgca atcaactttc gcggcattta
    15181 tcggtatgct agcaagtatt cttgtccttt tgtatttttt gaagaaacat caagcgtata
    15241 catcggcgtt tattcactac agcgaaaaca aaataaatat tgcaacgaag gaattgttgt
    15301 tagataccgt taaagaagcg attccgttta ttattgtggg ttcaggggta acgatcttca
    15361 aattagtcga tcagttcact tttatgaaga tcatgtctgc atccaccgag tattccaatg
    15421 cacagttact ggatcttttt tcgattttta gtgccaatcc agataagttg acgatggtag
    15481 ttattgcatt ggcaacatcg atcgcgtcta ctgggttacc gttgattaca gaagctgtta
    15541 cgttgaaaga tcgagtaggt ctggcaaaat tgacaagcgg aaatttacag ttgttttcat
    15601 tttttatgtt tccggcgaca tttggtgtta tgttacttgc ctatccgcta aatacattat
    15661 tttatacacc ggattctcta ggaagtaatg tactgatcca agcaagtttt gtaggactct
    15721 tcctaggact atatatgcta gtttccaaca tgctgcaagg aatgtttgaa aacaaggcgg
    15781 caattaatta tttggttatt gggtttctag tgaaactcct tcttcaatac ccagccattc
    15841 gtttatttga agtatacggc cctttattgg cgacaatgat cggatttgct gtgtcttgta
    15901 ccttgatctt aaagagaatc catgaagtag cccgctttaa accaagtttt gtctggcgca
    15961 ggacattgct gatctttatt cttaccttga ttatgctttt ggcttcgtgg atcacaaaga
    16021 tgatttttgg tgctttttta aacgaagata gcaaattcca gtctctattg ttgattcttg
    16081 tagtagcagg tataggggct ttagtgtatg gttatttagc tttgaaaatc cgcttggcag
    16141 aaagactact aggaccagga atagcgcgtt tacgacataa attaaaaata aaataagtga
    16201 atcagaagag ctagattgat gctcttttga aatgggggaa acgcatgcgt ttagataagt
    16261 ttttagcaga tatgggactt ggcagccgca aagaagtcaa aggattaatc aaaaaaggaa
    16321 cgatcgaagt caatggacaa gtggtgaaaa gcgataaagc gcaagtaaat gaattcgaag
    16381 atgcggttac ttatttagga gaaatcatta cgtatcaaaa agatttttac tacattttgc
    16441 ataaacctgc aggagtcatt tctgctactc aggataatca tgatcaaacc gtaatcgatt
    16501 tattggaaga ccaggattat cgggaagatc tttttccagt cggccgatta gataaagaca
    16561 cagagggcct cttgatttta acgaatgacg gggtgtttgc ccatcagtta ctatcaccta
    16621 aaaaacatgt ggagaaagaa tatctagctg aggttagcgg tataatgaca gaagaagata
    16681 tccgtttatt tgcagaagga ctgattattg atggcgatga gcagacattg ccggcaaaac
    16741 tccaaattga tcaagtagat caagagacag aaacttcaat tatccgtctg attttgcatg
    16801 aaggaaagtt tcaccaagtt aagcgaatgg tcaaagcagt aggaaaagaa gtcttatatt
    16861 taaaaagaat ccgtatggga aaattttggc tgcctaaaga tttgaataaa gggcaatatc
    16921 gacccatgac caaagaagag ctagaacaag tgagagaaaa ataattgaat ggatagctaa
    16981 aagaataagc agagtagttg tagcgccatc tgaaaagcag acttgtccgt ctgactttcg
    17041 gatggctttt ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg aacaccgttt
    17101 gttcggaata agggactgtg acaagacttt tgttacagtc cccattgttc cttgtgactc
    17161 agagttagat agtaaggaca cgatctccct aatactcagg ccaaaacgct gaattcacaa
    17221 cctctgataa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
    17281 atgaggaact attttcccgt ttttttctct aatactcagg ccaaaacgct gatttcacaa
    17341 cctctgataa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
    17401 atgaggaact attttcccgt ttttttctct aatactcagg ccaaaacgct gatttcacaa
    17461 cctctcctat actttgtttc attttcccta tactttgttt atttattctc attattgaaa
    17521 gcgctataat tacgttgcga atgaatcgaa aggggttttt agatgaaaaa acgaaagggg
    17581 ttcaatcatt tttttatgaa tctatggcgt tataaagcac ttgtcctgat ggctgtaccg
    17641 ggtatgcttt ggatgatttt tttcttttac atacctgtaa tggcgaatgt cgtagcattc
    17701 aaaaattttc atatttcagc agatgggttc ttggctagct tgagggaaag tccttgggtg
    17761 ggttgggaaa atttcaaatt tttattttct tcggatcaag catttctcat cactaaaaat
    17821 acaattttat ataatgtagc atttatcctt ttgaatctgc tgatctcagt cgtatttgca
    17881 atcattatga gcgagttgag aaataaacga ctcgttaaag tttatcaaac gatgtcctta
    17941 ctaccttatt ttctatcttg ggtcattatc agttactttg tttacgcttt tttaagtcct
    18001 gacaaaggaa tattcaatca gtggatcact ggacatggag gagaagctgt caattggtat
    18061 aatgaaccga aatattggcc gtttatcttg gtgttcattg gtacatggaa agggattggt
    18121 tataacagca ttatttactt tgcatctgtt atgggaatcg atccgactta ttatgaagct
    18181 gctatggttg atggagctag caaatggcag cagatcaaac atgtgacgat accacaactt
    18241 attccactaa tgacgatttt aacgatttta gccgtaggaa acattttccg agctgatttt
    18301 ggcctgtttt ataacattcc taggaattcc ggtgcattat atgatgtgac tcaagttttg
    18361 gatacgtata tctacaatgg cctgacatcc acaggagatt ttggtatgac ggctgctgca
    18421 ggactttacc aatcagttgt cggctttgtc ttgttgatga tcacaaatac gatcgcccga
    18481 cgttttgata gcgattctgc attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg
    18541 agagagtcca agtccgctct ttcaataaga cggctaattt tttctctaat ataatgattg
    18601 cactctttgc tatttcctgt attttgccat ttatatttgt tatcgccatc tcttttaccg
    18661 atgagtccat attgtcgact cacgggtaca gtttttggcc agaaaagttc agtatatttg
    18721 gttatacgta cttgttcgac cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg
    18781 tacttgttac tgttttaggt acactgatca atagcacggc tacttcttta tatgcttatg
    18841 cgatttcacg gtcaaacttt ccattccgtc gtttttttac cgttttttgt ttgattacga
    18901 tgttgttctc accagggatg gtggccaatt atcttgttat gacaaatctt cttcagttaa
    18961 aagatacgat ttgggcattg atcttgccaa tggctgtcag tcccttcaat attattgtga
    19021 tgcgcacatt ctttaaacgt tcggtctctg attcgattat tgaatcggca cggatcgatg
    19081 gagctagcga actgcgaata tttgttcaga tcgttttacc gttagctgtt ccaggtattg
    19141 cgacaatcag tttatttgcc gcattgggtt actggaatga ctggttcaat gctttactat
    19201 acatccaaga tgataagcta gttccactac agtatctttt gatgaaaatc caaagtaata
    19261 ttcaatatct gacacaaaat tcaggagcag gaagccaatt gacaggcgga ttagcctcag
    19321 ttccaggaga atctgccaga atggcgatcg tagtcatctc gacgttgcct attgcgatca
    19381 gctatccatt tttccagaag cattttgtca aaggtctaac aatcggcgga gtaaaagaat
    19441 aaagaaacaa aaatataaat cataaaatta ggaggaagaa aaatgaagtt atggaagaaa
    19501 ctagccttta caggtgtttc agcgttgttg ttgggaacat tagcagcatg cggaggttct
    19561 ggttctaaag accaggcaga agctagcaat tcggacactt tgcagatgta tcaaatcgga
    19621 gataaaccag ataatttcga tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag
    19681 atcggtgtaa aagtcaatat caattacatt ggctggggcg attacgaaaa gaaaatgaac
    19741 gtcatcattt cttccgggga aaattatgat atcgcctttg cgaacaacta tgtttccaat
    19801 gcccaaaaag gagcctttgc tgatttgact gaattagcgc ctaagtacgc tgaaaaagct
    19861 tataatgatt tagatgaagc atatattaaa ggaaatctgg tcaatggaaa attatatgct
    19921 ttcccagtca atggaaatgt ctttgctcaa caagtactta cttttaacaa agctttacta
    19981 gataaatacg atctttcaat tgatggaatc caatcttacg ctgatgcaga aaaagtcttg
    20041 aaggaattcc atgaaaaaga accaaacacg gcagcttttg ccattggaca aggcttcaaa
    20101 gtccaaggga actttgatta tcctttaggg aatacgttac catttgctat tgatctggat
    20161 ggagatgatt cgaaaatcat caaccaatac gataatgaaa cgtatatcgg actgttacgt
    20221 acaatgcata gctggtacaa acaaggactc attccaagtg acgctgcaac gagcaataag
    20281 gattatccac tagaaggaaa tacttggcta atgcgtggag aaacacaagg accgtatgat
    20341 tatggcgata caattttgac aaatgcagca aaacaagaac ttgtctcaaa agcaattact
    20401 gttccactaa aatctacagc tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca
    20461 aaaaataaag aaaaatcagt agaacttcta gggctgctta acagtgatcc agaattattg
    20521 aatgggcttg tttgggggat cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat
    20581 aaaatcaaat tgctagatgg ttatcagcca aatacacata tgtctgcttg gaatacagga
    20641 aacaacaaga ttctatatac acaagagtct attacggatg acatgattgc caaacgtgat
    20701 caatcgattg ctgatgcaga aacttcaccg attttaggat tcagcttcaa cactgacagt
    20761 gtcaaaacag aattaagtaa tatttctaat gtcatgaatc aatatttaga tggattgaat
    20821 acagggactg ttgatccaga tgaaacatta ccgaaattga aagacgcttt ggacaaggct
    20881 ggctatgata aggtattgaa agaaatgcaa aaacaatatg atgagttccg acaagaataa
    20941 taatgaataa agacaaaggg gttgtgacaa aagacgtgtc acaacctctt gttccgaata
    21001 aacggtgttc aaaagccagt ttctcggtat taagctaaat atatgtaaaa atgtggaaag
    21061 ccatattcac aaaatttttc ttactactcg aaactaagcg gctatttcac aaactcttgg
    21121 taaacggtgt tcaatcagct aaccctcggt attcgctcac aaaacgacaa aatatgagga
    21181 actatttttc cgtttgtttc tctaataatc aggtcaaggt gctgattgcc caaccttttt
    21241 ttgtataatg agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt
    21301 tggaagagtt atttatccgg atctgggtca ttataaaact aaccttgtat ttctggatat
    21361 atacatttgc cggcgggatc atctttggat taggtgcggc atggaaaaca gtcaatgaac
    21421 tattttattt gtacggattc gagtataaag aaatcacaat aaaaagaggc tggaatatat
    21481 ataaaagaaa ttttttaaga ggcaatctgc tatttagttt atttttatct ggtactgctt
    21541 tattgagtta caatttatat ttatctgttc aaatcaaagg gctcattttt ttagctattg
    21601 attttatttt actttttgcc ttgctctgcc tatttgctac ttatcaatac agtatgattt
    21661 tagatagtga atatacgatc tctgttccta atctgctaaa gctaagtttt atttcggttt
    21721 tttccagctt ttcgtctttt ctaaaaataa ttattggtag cggtataatt ttaggcgtga
    21781 cttggcaatt caaaggattg atcctgtttg gtgtcattgg tcttttgacg gtttggaatg
    21841 gtacgatgac aacacattgg cgtgaagaat tagataaaca gttggaaagt tatgaataag
    21901 tttcggattc tattttcaaa aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt
    21961 atcatgatcg gtgtaacgat gactgctacc gttattttta gtatccatac ttgggaacaa
    22021 aatcaaaaac aggcagaaaa catgacaagt gatgccgttc aaagcacaag cagaatgctg
    22081 aatgataaaa caaccttgag taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag
    22141 atcgaaaatg tgacgaccta cttaacaaaa cccatagatc agtatctgat gtatgtatac
    22201 gaacagcaaa acagtaccga tgaattagtt tctttcccaa atcaaatcaa agatctctac
    22261 gcaaattatg aagaactaag tgcgatatat atcgtgttga atcaactacc ggagtattat
    22321 gaatcttcta gagaaaacaa aggtggggaa ataaaaaagg ggacaccaaa attactagac
    22381 gccttttata tcaagttgcc tatcacacaa ggaggagctg agtcaggaag tatttttatt
    22441 ggttttcaaa agaaagaact agatatgatt ttagaaaatc taacgtcatt caatggactc
    22501 tctttataca tgatttctgg aacgacaaat cgactataca cattccatga taataaaata
    22561 gctaaagaaa cgtttgaaag acaagaaatg atcatcacag agagtctgaa gaaaaattca
    22621 caattgccga ttgacgatct ggaaaaaaac aattttgttc aacaccaaga actgtctgat
    22681 gattatcgta tattagctgt actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat
    22741 cttgccccgc tgatcattgg tatcattatt ttagacaata tactgcttat ctttctttat
    22801 aaaacattca aacgatattc acagcaagta tctatcgtca tgcaggccat ggatcaaaca
    22861 gcagcaggga atttagagac gcggatcgac ataacaaaca cggaatatga actgaaggaa
    22921 ctttcaatcg gtatcaacga aatgttggat agcatcaatc agtttgtaga agacatctac
    22981 aaattagaaa tcaaacagca agacgcacat atgcgggcgc ttcaagcaca gatcaatcca
    23041 cactttttat ataatacatt agaatatatc cgaatgtatg caatcagcga aggaagtgaa
    23101 gaattagcag atgttgtgta tgcgttttcc gctttgcttc gaaacaatac gaatcaagag
    23161 aaaacgatca ctttaaaaga agaactggac ttttgcgaaa aatatgtcta tttgtatcaa
    23221 atgcgttatc ctaatcgagt agcctatcat tttatgatcg atcctgattt ggaaaagatc
    23281 gaagtaccta aatttgtgat ccagccatta gttgaaaatt attttaaaca cggtattgat
    23341 tttactcgat tcgataatgc gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg
    23401 atcatcatca aagataatgg aaaagggatg actgaaaaac ggctaaaaca gattgaagaa
    23461 aaactgtctc atcctaaagt ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa
    23521 cgtttacgtg ccagcttcgg cagcagttat tatatgtcgc ttgagaataa tgagacaggt
    23581 ggactgaccg tttctattac attcaaggag ggatgagcgt gtatcaagtg ttattggtag
    23641 atgatgaata catgattttg aatgggttaa aaaagataat tgattggcaa agtttaggct
    23701 ttcaaatagt agcaacagca gagaatgcca aagaaggact agctgtactt gaacagcgac
    23761 agattgattt agtagtaacg gatgtgacaa tgccagaaat aaatggactg gaatttattg
    23821 aagcggctca aaaagaacgt cataattttg aatttatgat tttatcagga taccaagaat
    23881 tcgattattt gaaaggaggt atgcaattag gcgccgtgaa ttatttgatg aaacccgtga
    23941 ataagttcga attggttgaa agcctcaaga aaatcaaaac acggttagat cagcaaaatg
    24001 aacaaaaaaa tcaacaagaa atctatcaag aactgttatt cagtcaatgg ttgaatgaag
    24061 aattggatga aataagtgaa gaagaaataa taggacaaat aagtgagaag cagcatagga
    24121 tagtattgat tcaactggca agaactcatg atactcttat caatagatgg ttgcaagaac
    24181 ggcaacaaat cttttactat caacgaaatt acggagaccg tgtattgatc acgttgttgt
    24241 tggaggcaga tgaagttgat ggcttctgtt gttttgttca agaaaacttt cctgatcagg
    24301 aatggctgat cagtatcggg gaagagacag attctttgga caaaatccct gaaacattcc
    24361 aacaagcgaa agacaatcta cagttgcatc aattttatgg agataaagac gagcatgttt
    24421 tttacgcagt acaagcagaa acaaaagatc aaaccatcga tttttcaagt ttcagacgcg
    24481 tgctgcaaaa taaacaattg gaagtcgctc aaaaaatgat cactgatttt tttgaacagt
    24541 ttcagttagc agccatgatg ccggaagata tacgatattc cagtttttta ctgtttatgg
    24601 agattcaacg ggaattgatt gatctggaag atgaggaata tttacaagga atcgaaaaaa
    24661 tccatcaggc aaaaacggtg caagaacttc agcagctact tctgtcattc atcaaaaagc
    24721 atcagagaca gaaaaagtac agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc
    24781 attatcaaga accacttaca ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt
    24841 atctaggaca gctgttcaaa aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt
    24901 tacgtatgga aaaagcgaaa caactgcttt tgcacagtaa tcaaaacatc aatgagatag
    24961 caagtgagat tggctacaac aacaccacgt atttctctaa attattcaag aaaatcgtcg
    25021 gcagatcccc taaagagtac cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt
    25081 aatgaaataa cttataaaaa attgtctaga aatctaattt tgggctgttc ttattacttg
    25141 aggcaaggca gctttttcat gacctctcga taaacggagt tcaaaagcca gtttctcggt
    25201 attaagccaa atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttaatact
    25261 cgaaactaaa cagctttttc acgacctctc cactaaacgg tgttcaatca gctgaccctc
    25321 ggtattagct caaaaaatgg caaaatatga ggaactattt tgccatttgt ctctctaata
    25381 ctcaggtcag aacgctgatt tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct
    25441 gcggtaataa gaccaaccaa acgaaaaata tgaggatcta tttttgcttg gctgttctta
    25501 ttacttgatg caggacagct ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa
    25561 aggcaaatct tttgcttgat atttcttagg gtgagggatg atccctaatt ggtaagagat
    25621 cgtgccgcct ttcagcaaat cttgatgggt aaaatataat ttcgtatatt ctttgtccat
    25681 ccgtttgatt ttatggataa attgttgctg cggccgatta ggcgttgctt caatcgtcaa
    25741 ttcctttcca tttgacaaaa gcaaaacagc cttgtcaatt aatggcatac cgataacata
    25801 ttcaccggtt ccaggcgtga caggataaaa tcccagacta ttgaagatat accaagcggc
    25861 catacttcca ttgtcctcat ctccagggta acccattgga gaatcgtcga aggtctgagt
    25921 catcaactgt ttaagcagtg gctgtgccat ttcaggttgt cctatataac tgaacaagta
    25981 aggatagtga aaacttggct ggtttgaaat agctaattgt ccaaattcta tcgcggccat
    26041 ctcactcatt tcgtggatct caaaaccata tcctcctggg tcaaacgttg ggggctgatt
    26101 gcacagctca atgagttttt ctttatatct ctcactgcca ccatattttc cgatcagtcc
    26161 tgaaaaatcg tgataaacag caaagctgct ttgccaagca cttccttctg cataatcttg
    26221 tccccaacga ttagctgaaa atggttctct gaacttgcca tctttatctt ttgaacgcat
    26281 aaatcctgac tcatagtcaa agatattttg atagttcaat gattgctgct gatatttctt
    26341 cgtcatttcc tcttcttcta gtaccttggc aacttggcta atacaaaaat cgctataagc
    26401 atagtctaat gtgtgattga ctgattcgtg gtagtgatta ggaacatagc catatgtcaa
    26461 gtaatcttct gttccttggc gtccataatt tggatgtttg ctttgaaccg tagcaccttt
    26521 ttccattgcc gttagaaatt caggcatcaa gtctcgtcgg atatctttta cagcagcatc
    26581 tgcaatgacc gcatcgatca atgtcccagg cataagtcca cgttcatcag gagacagcca
    26641 ttttggcaaa tagccacttt ctctatagct gttcaaaaaa ccttccagca tttcttcgta
    26701 tttttctaca gcaatcaaag aataaagagg ataaaccgtt ttatacgtat cccaaaaccc
    26761 attatttgta tagagaacac caggttttat agatcgacta gtcgtatcat agtggatttt
    26821 ttgcccacta ggatcaattt cataaaatgt ttgtggaaat aaaaacaccc gatataaatt
    26881 atgataaaac gtccgcaatt gttgttgatc atggtgtgtg agctgtatac gatcaaaata
    26941 ggactgccat tgttcttgac tgtgagcaag gtaagtctct ttgctttggt tttgttctag
    27001 ttccaggtta taagctgctt gttccgagct gataaaagat gtcccaaatc ggatagtttg
    27061 tttttttgtc tgttcaaaat acaacgggat cgagtcgcta tttttgttgg aaacagctaa
    27121 ctcatcaatt gttaaaggat attcaaatct caatataaaa taaaaagaaa aatcaggatc
    27181 ttctgaacca gcaaaatgtg tgatttttcc ttccactgtg taaggatcta taacaaagag
    27241 ctgatggcgt cccggaaagg ataaaagtaa accgctgtca tttttgctgt aatcaataga
    27301 taagattccg ccatacatgc taggtatcaa tgtagaacag atgccataac gtaattgaca
    27361 gatcgacaga tgagtcgggc aaaatatact ttcttctggg cggtaagaac tttgagcatg
    27421 gaaaagggtg ttctcctgta gttttccatt gatcggcgta aacagcatat gactgaaatc
    27481 acccatccaa ggacttggtt gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg
    27541 atgaaaccac cagctgccgt tttgatccgt agtttgcggt gcaaaataat tcatcccaaa
    27601 aggaacgcca gtataaggct ggcagttccc attagagaaa tttggctgat tcgctgttgc
    27661 atgtctagta tctatttgac cgatattcat tttatttctc ctttgttgat tgtcttttga
    27721 tgaatgaaac tggaaattta ctatcggatg gtcgatcttt tgtctctaac caagtgacca
    27781 aagcatctcc cgcaagctta cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg
    27841 ggtcaacaag agctgctgct tggatatcat caaatccgat aattgaaaaa tcttcaggga
    27901 tatgataacc atgctgtctc aagagattca tggtctggat agcgacaaca tcgttttcac
    27961 aaacaaaagc agttacttca tacttagtga tataagaaag caattcatct aatgtcgcgc
    28021 ttttgtcatc cagtgaagtg tggaaagtca aaccagcttt gtccaatgct tcaaggtaac
    28081 caaggtaacg ctgtcgaacg gattgtggat gagtctgcgt accaaacaaa taagcgattt
    28141 ttgaatgtcc ctgttcgatc aaataagagg cagcaagcgc accgccttgg aaattatcgg
    28201 aaagaacggt aggaaaaggt aactcataga tttttttatc taatatgacg acagggaatt
    28261 ctttcaatga aagctcaaaa agtaagtcaa gatgttgttc tgtatcaaga gcataataaa
    28321 tcaatccgtc aaattcattg attagctctt ttgctgtttt attttgtaga aagtcaagcg
    28381 tcgtcatcat gacatcaatc tgtttatctt gcatcacagg gttcaatccc ttgctaaaat
    28441 caccaacaga caaatcagcg acatatggaa gaagaaacag aatccgtcgt gtttttgtag
    28501 gagaagcaag aatctgttcc ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc
    28561 gataaataag tcctaattgc tctaattcag tcaatgcacg tttggaagtg attctgctta
    28621 ctttataggt acgtgaaagt tctttttcag ttggtagttg gctgtctaca ggtaattcac
    28681 cagttaaaat agccgtttgc aaatcattaa atattgtctg gtacaatggt tgattcatat
    28741 aaacacctct taatgatata tcatattcaa ataataccat aatggatgaa aaagtaaata
    28801 aaaagatatt ttttgaaagc gtagacataa ttggctgtat gaagtataat ctttaaagat
    28861 atatcaaata gaaagaaggt tttattagtg gcttataaag atataccaaa atcggtgcag
    28921 aaatttatgg aagatatcac acaaaaatgt ggggatatcc atgcggactg ggcaaagaat
    28981 tttacagcag catttgccaa tactctgttg acaacagtca aaaagcaaaa tgacggaacc
    29041 acctttttat tgacgggaga cattccagcg atgtggttaa gagactctac tgcacaagtt
    29101 cgtccttatt tagtgattgc aaaagaagat gaggaattag ctgacatgat cagcgggtta
    29161 gtcaaaaagc aattttttta tatcaatatc gatccatatg cgaatgcctt caacgaagaa
    29221 gctaacggtg ctggccacca aacagatcat acaaaaatga acgattggat ctgggaaaga
    29281 aaatatgaaa ttgattcttt atgctaccca atacagttgg cctatcttct ttacaaaaat
    29341 acaggaaaaa ctgatcaatt caatcaatct tttattgaag gtatcaaaaa aatcttggac
    29401 gtgttcaaaa cagagcaaga tcatgaacac tctccttata catttgtacg agacacgaca
    29461 agaaaagaag atacgctgat caatgacggg cgaggtgcaa aaacagcttt tacaggaatg
    29521 acatggtctg gtttccgtcc tagcgatgat acttgccgtt atggatatct ggtaccatcc
    29581 aatatgtttg cggtggtcgt gttaggttat gtggaagaaa tttttcaaac gatactcgat
    29641 gataaagaaa ttgtacagca aacacagaaa ttgaagaaag agatccaaga ggggattcag
    29701 aatcacggac gaacggttaa tcaaaaaggg caaacgattt atgcatatga agtagatgga
    29761 atgggaaacg ccacagtcat ggatgacagt aatgtgccca atctgatcgc cgcaccttac
    29821 ttgggatatt gttctgaaca agaggaagat tatctgcaaa caagaagaac attgttgagt
    29881 aaagaaaatc catatttcta tgaaggaaag tacgcaaaag gaattggatc ctcccatacg
    29941 ccagaaaaat acgtttggcc gattgcatta gctatggaag gaatgacaac gtcagacaaa
    30001 tctgaaaaag agcggatatt gaatttactt gtagcaactg acgcaggaac acaccttatg
    30061 cacgaaggat tcgatgtgaa caatcctgaa aattatacga gagaatggtt ttcttgggca
    30121 aatatgatgt tttgcgaatt agtcatggat tactttgata ttagagtaga atagtaggag
    30181 gtatcgtaaa tgaaaaagaa agtatatatc atttctcata gccactggga tcgtgaatgg
    30241 tacatggctt acgaacagca tcacatgcga ctgattgaat tgatagacga tctactagag
    30301 ctatttgaag ttgatccgtc cttcaacagt tttcatttgg atgggcaaac gattatttta
    30361 gatgactatc ttcaagtaag gccagaaaaa agacaggcaa tccaacaagc aatcaatgaa
    30421 ggaaaattga gaattggacc attttatatc ttgcaagatg attttcttat cagctcggaa
    30481 tcgaacgtga gaaatatgct gattggcatg gaagaaagcc gtaaatgggg aacccctgtc
    30541 atgttaggct attttcccga tacatttgga aatatgggac agacgcctca attaatgaaa
    30601 caagctggta tttcagcagc agcgtttggc cgtggtgtaa aaccaattgg ctttgataat
    30661 caagttttgg aagcagaaaa ctattcttcc caatactcag agatgtggtg gaaaggacct
    30721 gatcaaacag caatctttgg tctgctattt gccaattggt atagcaacgg gaatgagatt
    30781 ccagtagaaa aagaagcagc tcttgccttt tggaagcaaa aattagcgga tgcagaacaa
    30841 tatgcctcta cgaaccactt attgatgatg aatggtgttg atcatcaacc ggtacaaaaa
    30901 gatatatcaa aagcaatcca tctcgcaaat gaactgtttc cagattacga gtttattcat
    30961 tctaatttta ctgactatct ggaagcagtt caaaaagatg tgccagaaga cttaggctca
    31021 gtagaaggag aattgacaag tcaagaaacg gatggctggt atactttagc caatacagca
    31081 tctgctcggg tttacttaaa acaatggaat accaaggtcc agcgacagct agaaaatatt
    31141 acagaaccac ttgcgacgat ggcttatgaa gttagtggaa actatcccca tgatcaatta
    31201 gattatgctt ggaagacatt aatgcaaaat catcctcacg acagtatttg cggttgttca
    31261 gtcgattctg tccatcggga aatgatacct cgttttgaaa aagcagatga agttggaaag
    31321 tatcttgcac aagattcctt agaacagctg accgcagcaa tcgatacgac cggattccca
    31381 aaagacagct ttccatttgt cattgtaaat actgcaggaa tggataaaac tggtgaagca
    31441 gaaataacca tcgaattaga aagaaaaaga ttcgctgaag gaatacctga acaactatat
    31501 caagagttag aaaatttgcc taaacgtaag tatcatgtag aaacaaaaag tggggccact
    31561 ataccagcta ttttatctga agaaactgtc caatttggtt atgatttgcc gaaagaccgt
    31621 ttccgtgtgc catatatggc aagaatgatc aaagtgactt taccattaga aaacatgcca
    31681 gcattttctt gggaaacatt cgcgcttgta gaaggggaag cgaaagcaga agaaaaggaa
    31741 acaatgattc atcagagtgg acgaatcatc gaaaacggcc ctctacattt gacgattgaa
    31801 aaaaatggta ccattacaat ggaagatcgc aaaaacaaac gaaagttgaa taatctgcat
    31861 atttttgaag atatcggcga tataggaaat gagtatatct ttaaacaacc tttctgtgat
    31921 cagccaatcc tatccagtaa caaagaaaac tctgaagtaa aagtattagt agatacaccg
    31981 gaaatagcaa aaataagtat tctacaggaa atggaaatcc ctgtttctgc agacgagcgt
    32041 ttagaaaaag aacagcagat ggttgtggaa tttcgttatc gaaaagcaga acgatccaaa
    32101 gaaaaacgaa tccttcaaat aaaaacaatc atgacaatca gaaaagatag taaaaaaatc
    32161 gactttgaaa cgactctcga caatcagatg aaagatcatc gcttacgtgt cttgtttcct
    32221 acaaagttac atgtggaaca tcacgaagca gatagtattt ttgaagtggt gaagcgccca
    32281 aaccatgtgt ctaaaagttg ggaaaacccc acgaatccgc aacatcagca agcatttgtc
    32341 aatattcatg atgaagagta cggtgtaaca gtcggaaact ttggtttgaa tgaatatgaa
    32401 gtaacagaag acggacaaat cgcagtgacg ctacttcgca gtgtgggaga actaggcgat
    32461 tggggatatt ttccaacacc agaagcacag tgtctaggtg agcatcggtt caattacagc
    32521 attgaactac atggaccaga agaaaaattc tctacttatt tgcatgcata cgcagcacag
    32581 attccttttt ccactcaaca aatcaaacat catgaaggaa cattgatttc aaaacaacaa
    32641 tatttaacga taaaaagtga aacgtttgca attacagctt tgaaacgcag caagtttagt
    32701 gataaagtag tagtaagagg tttcaatatg tctagtcatt tagaaaaatt ggaaataact
    32761 aaagacaatg gaaaaactgt catattgaat ctgcttgaag aaccaacgaa gcaagcggtt
    32821 gtcccaataa tccaacctta tgagattcgg acaatcggat tcgaggagga aaacgaatgt
    32881 atggcggaat agaagcaggc ggaacaaaat ttgtgtgtgc ggtttcagat ggtggagaaa
    32941 ttttagaaaa attaagtatc cctaccacaa caccagaaga aacattggat caggtatttg
    33001 aattttttga tcgttatgaa ttgaagggaa tcgggattgg ctcattcggc ccgattggta
    33061 tcgacaaaac aaaagataat tacggatacg ttttggcaac tcccaaaaaa gggtgggcac
    33121 aattcgattt tcttggcaca atcaaggaac gctacaatat tcctgctgct tggacaacgg
    33181 atgtcaatgc tgctgcatat ggggaattga agcaaggagc agcacaagga aaaaatagtt
    33241 gtatttacct taccgtggga acaggaatcg gtgcaggtgt cgtgatcaat gaagaaattt
    33301 tttcagggat cgcacatcct gaaatgggac atatttgggt gaaacgccat ccagatgacc
    33361 attatgaagg tacttgtcct tatcataaag attgtttaga aggattagca gcaggaccgt
    33421 ctattgaggc acgcacaggg atcaaagggc aagatctgcc gcaagatcat cctgtttggg
    33481 ctattcaggc ttattatatt gctcaagcac tgatcaacta tacgttgacg ttagcacctg
    33541 aaaagattat tttaggcggt ggggtaatga atcaggatca tctccttcaa aaaatccgtc
    33601 agcaatttgt tgaattgatg ggtggttata tggaaacacc tcaagtaagt gaatatatcg
    33661 ttcgttgggg actgccgaat gaaagtggga ttatcggtag tttgctgtta gcagaacacg
    33721 aacatcaaaa gtaagcagac aaattacaca aaaagaacag agtcttatca ttatctgctg
    33781 ataagactct gttctttttt atttctattc ccataatcca ggtaaagctt taccgactat
    33841 tttttcttga gtaaatggat ggatcatgtg cagttctaat gcatggagca tcagtcgttc
    33901 agctgttcta ttttggtaga gagggtctcc aacgatcgga tgtcccttat gagctaaatg
    33961 aactctgatc tggtgtgtac gtccggtttc caaaacacaa tagacttgtg tttgttcaga
    34021 aagtactttg ttgacagtga catgtgtcaa ggcgctctga ccatttttat catctacgac
    34081 cctttttctc cgatcatggc gatctcgtcc aattttattg gtgatggtca tatctttggc
    34141 aatttctccc catacgatgg cttgatatct ccgatagatc attttttgtt ccaacattct
    34201 tccaagtatc ggcaagacaa atggattttt tgcaaataaa acggcaccgc ttgtttcttt
    34261 gtccaagcgg tggacaacgt aaggtcgttg ctgtttagtg tcgagataag cagctaaatg
    34321 attgagtaag gtactgtttt ctgtcggttc atttggatga gtcttcattc ctgcaggctt
    34381 atccactata atcaaatgct catcttcata taaaacacgg atgtgcttcg catctcctaa
    34441 ttgaacttct tgataattat aatctgtttc ttcgaataac aatgtgatct ggtcattgcc
    34501 ctttacttca tgatggaaca aagcaggttg ttgattgatc caaacgtttt tacgtatacg
    34561 caaaaagtgt cgaacctttc taggaactaa ccattccttt tcaagcaagt cgcgaattgt
    34621 tgtttcagga tggttcatag gtaaagtgat cgtgattttc acaagtgtac tcctttcagt
    34681 gcaatcgcag actaattgta ccatttttaa aacatattta agaaaaaact tacatttttc
    34741 tgttcattct tatgttactc tacttgcact aacgtaaaaa aacatattga actacaactt
    34801 aagcaggaag aaaacactct ttcactatgg taaaataaga cgattgaatc aaatagacag
    34861 gagaagaagc aaatggattt tcaaagcatt ttagaaaaga taaaagctgc tttgatcagt
    34921 ttttggcgtt ggatcagacc atatctcgga caattccatc gatggcgaaa gcgcatctgg
    34981 aaaaaatatc atgtgaacaa attgattttg ctgattggtt tgatctgtgt cttagtgaca
    35041 agtatctacc tctttatttt agcaaaacaa gcaaatgtcg aaacgttaaa atcaggatta
    35101 agccaatcga cagtcattta tgataagaaa aacgaagaag ctggcacact gtacgcccaa
    35161 aaaggaacat atgtagaatt aaacgcgatt tctccctata tccaagatgc agtaatctcg
    35221 acagaagata ggaattttta cgagcatcac gggtttgata tcaaagggat tgcccgtgca
    35281 gcagtccgaa tggtcttgtc tggcggaaca tcagggggcg gaggaagtac gatcactcag
    35341 cagcttgcca aaaatgcgta tctgacatta gaccaaacgt ttgatcgaaa agcgaaggaa
    35401 ttatttttgg caatcgagat cgaaaagaag tacagcaaag aagaaatctt gaccatgtat
    35461 cttaacaatg cctattttgg caacggggta tggggtgtcc aagacgcttc cagaaagtat
    35521 tttggagtag atgcaacgaa tgttacgctg tctgaggctg ctactttggc aggaatgctg
    35581 aaaggtcctg gtatttataa tccgatcgat cacccaaaaa atgcgaatga tcggaaaaac
    35641 acagtactaa gtgtaatgga agaaaacgga aaaatcacaa aagaacaaga acaagcagaa
    35701 agccagacag atatcagcgc gtatcttaat gacacatacg ataattcaga ttctggttat
    35761 cgttatcctt attattttga tgcagtgatc gatgaagcag ttaacgagta tggtcttgat
    35821 gaagaagatg tcatgaataa aggctataaa atctatacag ctttagatca ggattatcaa
    35881 aagcaattag aagctactta tcaaaacagt gcattattcc cagcaaatgc ttcagatggt
    35941 gccatggtcc aatctgcttc agtagcttta gatccgaata caggtggtgt acaagcacta
    36001 gtaggacgaa gaggagatca tgtctttaga ggattcagct ttgctacaca gatgcgtcgt
    36061 tcaccaggat caacgatcaa accattaagc gtatatacac cagcactgga agctggatac
    36121 aagccttcca gtatcttgaa agacgaaccg caaagttatt acgatgcgca taattttgac
    36181 gggacatacc aaggagaagt accgatgtat caagctgttg cacaaagttt gaatttgcct
    36241 actgtatggc ttttgcatga gatcggttta caaaaaggat atgataaagc agaagaattc
    36301 ggcctgcctt tagcaaaatc tgataagtat tatggcttag cccttggtgg actggaaaaa
    36361 ggagtatctc cattgatcat ggcgggtgct tacagtgcat ttgccaatga tggtcagatg
    36421 tatacgccgc atcttatcac gaaaatcgtt gattcgactg gagcggtgat cgtcgataac
    36481 acaaacaaaa aaccaaaaca ggtcatatca aaggaaacag ccgatgaaat gaccagcatg
    36541 cttcttggta cattttcaaa tggaacagcg gctgctgcga atcctgcagg ttatacgata
    36601 gcagggaaaa caggaaccac agaaactaat ttcgatgcca caaaagcaaa tgaccaatgg
    36661 atgatcggtt acacgccaga cgtagtgatt tcgacatgga tgggttacga agaatccagc
    36721 aagctgcact atttggaagg gacaagtgga aatgttgtcg ggaaagtctt caaatcagcg
    36781 gcagaaggca tccttccgta tacttcaaaa acaggattta gtgtagcaga tgcctatgcc
    36841 acaggaggac aagtggtagc tgcagatcaa gttggaaaca caggaaattc taatggagag
    36901 tctggaaatt ggcaagataa cttgaatcaa tatggtgaac aagcaaaaga agggctgaaa
    36961 aacttcggcg atatggtgaa agaaggcgtt cagggaattg gtgaagcggc taaagacctc
    37021 tggcggaaat atcaaggaga gtagcccctt tgaaaacgaa taaccccatg ttacaataga
    37081 gagtaactac taaaagaaaa gagggcataa catgtcaaac atctacgata gcgcgaatca
    37141 aatcgaacgt gagatccgtg aattaccaga attcaaagcg ttgaaagaag cgtacgagaa
    37201 agtacaagca aacgaagaag cacacaaact gttcaaagac ttccaagcga tgcaaatcga
    37261 attacaagaa aaacaaatga gtggacaaga gttcagcgac gaagatgcaa tgaaagcaca
    37321 agaaatggct gtgaaaatcc aatcagaaga agtaatcaat gacttgatgc aaaaagagca
    37381 aggcttcagt acaatcatca atgacttgaa ccgtatcatc atgacaccag ttcgcgacct
    37441 ttacagcgaa taaaactatt ttatgaaaca acagagggtg tgaaagagac gttcggcctt
    37501 gagcgatgac aaaattgctt cttattgccg ataggctgtc tcttgaacac catttaccaa
    37561 gaggtcgtga gcctaccgtt taactctgag ttacaaggaa caatttcaaa aaacagcttc
    37621 tcatgtttga tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt
    37681 cagaaatagg agctacgaca aaagtcttgt catagctcc
//
LOCUS       DACDVT010000022        37644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDVT010000022 DACDVT010000000
VERSION     DACDVT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.022"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IWL52_001110"
     CDS             complement(148..2301)
                     /locus_tag="IWL52_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9777686.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="IWL52_001111"
     CDS             complement(2595..3443)
                     /locus_tag="IWL52_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9777687.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWL52_001112"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWL52_001112"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9777688.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="IWL52_001113"
     CDS             complement(5944..6123)
                     /locus_tag="IWL52_001113"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9777689.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWL52_001114"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWL52_001114"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9777690.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="IWL52_001115"
     CDS             8271..10550
                     /locus_tag="IWL52_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9777691.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="IWL52_001116"
     CDS             complement(10643..11290)
                     /locus_tag="IWL52_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777692.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="IWL52_001117"
     CDS             complement(11469..12188)
                     /locus_tag="IWL52_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9777693.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="IWL52_001118"
     CDS             complement(12319..13470)
                     /locus_tag="IWL52_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9777694.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="IWL52_001119"
     CDS             complement(13687..14427)
                     /locus_tag="IWL52_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9777695.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWL52_001120"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWL52_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9777696.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /locus_tag="IWL52_001121"
     CDS             complement(14811..15404)
                     /locus_tag="IWL52_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9777697.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16035)
                     /locus_tag="IWL52_001122"
     CDS             complement(15388..16035)
                     /locus_tag="IWL52_001122"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9777698.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWL52_001123"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWL52_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9777699.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWL52_001124"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWL52_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9777700.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="IWL52_001125"
     CDS             complement(17481..18008)
                     /locus_tag="IWL52_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9777701.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="IWL52_001126"
     CDS             complement(18188..18973)
                     /locus_tag="IWL52_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9777702.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="IWL52_001127"
     CDS             complement(18966..19835)
                     /locus_tag="IWL52_001127"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9777703.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="IWL52_001128"
     CDS             complement(19836..21215)
                     /locus_tag="IWL52_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9777704.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWL52_001129"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWL52_001129"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9777705.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWL52_001130"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWL52_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9777706.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWL52_001131"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWL52_001131"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9777707.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGTKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWL52_001132"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWL52_001132"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9777708.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWL52_001133"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWL52_001133"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9777709.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="IWL52_001134"
     CDS             complement(25173..25397)
                     /locus_tag="IWL52_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9777710.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="IWL52_001135"
     CDS             complement(25450..26412)
                     /locus_tag="IWL52_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9777711.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="IWL52_001136"
     CDS             complement(26422..26862)
                     /locus_tag="IWL52_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9777712.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="IWL52_001137"
     CDS             27168..28133
                     /locus_tag="IWL52_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9777713.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="IWL52_001138"
     CDS             complement(28190..28420)
                     /locus_tag="IWL52_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9777714.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="IWL52_001139"
     CDS             complement(28506..29393)
                     /locus_tag="IWL52_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9777715.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="IWL52_001140"
     CDS             complement(29482..30705)
                     /locus_tag="IWL52_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9777716.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31801)
                     /locus_tag="IWL52_001141"
     CDS             complement(30722..31801)
                     /locus_tag="IWL52_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9777717.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWL52_001142"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWL52_001142"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9777718.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="IWL52_001143"
     CDS             complement(33659..33925)
                     /locus_tag="IWL52_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9777719.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="IWL52_001144"
     CDS             34404..36638
                     /locus_tag="IWL52_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9777720.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="IWL52_001145"
     CDS             36786..37106
                     /locus_tag="IWL52_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9777721.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="IWL52_001146"
     CDS             complement(37311..37535)
                     /locus_tag="IWL52_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9777722.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT        10564 a         8133 c         6540 g        12407 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgta cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattcctttt cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaat
//
LOCUS       DACDVT010000023        36791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDVT010000023 DACDVT010000000
VERSION     DACDVT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.023"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            54..500
                     /locus_tag="IWL52_001147"
     CDS             54..500
                     /locus_tag="IWL52_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9777723.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(559..1020)
                     /gene="mscL"
                     /locus_tag="IWL52_001148"
     CDS             complement(559..1020)
                     /gene="mscL"
                     /locus_tag="IWL52_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9777724.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            1272..2540
                     /locus_tag="IWL52_001149"
     CDS             1272..2540
                     /locus_tag="IWL52_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9777725.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2533..3819
                     /locus_tag="IWL52_001150"
     CDS             2533..3819
                     /locus_tag="IWL52_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9777726.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4597..5481
                     /locus_tag="IWL52_001151"
     CDS             4597..5481
                     /locus_tag="IWL52_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9777727.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            5690..6268
                     /gene="pgsA"
                     /locus_tag="IWL52_001152"
     CDS             5690..6268
                     /gene="pgsA"
                     /locus_tag="IWL52_001152"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9777728.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            6508..7749
                     /locus_tag="IWL52_001153"
     CDS             6508..7749
                     /locus_tag="IWL52_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9777729.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            7858..8907
                     /gene="recA"
                     /locus_tag="IWL52_001154"
     CDS             7858..8907
                     /gene="recA"
                     /locus_tag="IWL52_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9777730.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            9318..10874
                     /gene="rny"
                     /locus_tag="IWL52_001155"
     CDS             9318..10874
                     /gene="rny"
                     /locus_tag="IWL52_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9777731.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10953..11759)
                     /gene="proC"
                     /locus_tag="IWL52_001156"
     CDS             complement(10953..11759)
                     /gene="proC"
                     /locus_tag="IWL52_001156"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9777732.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            11900..14473
                     /gene="mprF"
                     /locus_tag="IWL52_001157"
     CDS             11900..14473
                     /gene="mprF"
                     /locus_tag="IWL52_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9777733.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            14627..15478
                     /locus_tag="IWL52_001158"
     CDS             14627..15478
                     /locus_tag="IWL52_001158"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9777734.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            15723..16661
                     /locus_tag="IWL52_001159"
     CDS             15723..16661
                     /locus_tag="IWL52_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9777735.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            16684..17364
                     /locus_tag="IWL52_001160"
     CDS             16684..17364
                     /locus_tag="IWL52_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9777736.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            17337..18161
                     /locus_tag="IWL52_001161"
     CDS             17337..18161
                     /locus_tag="IWL52_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9777737.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            18328..19146
                     /gene="purR"
                     /locus_tag="IWL52_001162"
     CDS             18328..19146
                     /gene="purR"
                     /locus_tag="IWL52_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9777738.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            19649..21022
                     /gene="glmU"
                     /locus_tag="IWL52_001163"
     CDS             19649..21022
                     /gene="glmU"
                     /locus_tag="IWL52_001163"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9777739.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            21116..22087
                     /locus_tag="IWL52_001164"
     CDS             21116..22087
                     /locus_tag="IWL52_001164"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9777740.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      22093..22195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22261..22830
                     /gene="thiT"
                     /locus_tag="IWL52_001165"
     CDS             22261..22830
                     /gene="thiT"
                     /locus_tag="IWL52_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9777741.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(22911..23684)
                     /locus_tag="IWL52_001166"
     CDS             complement(22911..23684)
                     /locus_tag="IWL52_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777742.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            23950..25650
                     /locus_tag="IWL52_001167"
     CDS             23950..25650
                     /locus_tag="IWL52_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9777743.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            25620..25838
                     /locus_tag="IWL52_001168"
     CDS             25620..25838
                     /locus_tag="IWL52_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777744.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            25858..26748
                     /locus_tag="IWL52_001169"
     CDS             25858..26748
                     /locus_tag="IWL52_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9777745.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            26766..27050
                     /locus_tag="IWL52_001170"
     CDS             26766..27050
                     /locus_tag="IWL52_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777746.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            27537..28151
                     /gene="gmk"
                     /locus_tag="IWL52_001171"
     CDS             27537..28151
                     /gene="gmk"
                     /locus_tag="IWL52_001171"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9777747.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            28156..28461
                     /locus_tag="IWL52_001172"
     CDS             28156..28461
                     /locus_tag="IWL52_001172"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9777748.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            28714..31122
                     /gene="priA"
                     /locus_tag="IWL52_001173"
     CDS             28714..31122
                     /gene="priA"
                     /locus_tag="IWL52_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9777749.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            31137..31628
                     /gene="def"
                     /locus_tag="IWL52_001174"
     CDS             31137..31628
                     /gene="def"
                     /locus_tag="IWL52_001174"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9777750.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            31621..32559
                     /locus_tag="IWL52_001175"
     CDS             31621..32559
                     /locus_tag="IWL52_001175"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9777751.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            32559..33917
                     /gene="rsmB"
                     /locus_tag="IWL52_001176"
     CDS             32559..33917
                     /gene="rsmB"
                     /locus_tag="IWL52_001176"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9777752.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            33930..34670
                     /locus_tag="IWL52_001177"
     CDS             33930..34670
                     /locus_tag="IWL52_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9777753.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            34667..36736
                     /gene="pknB"
                     /locus_tag="IWL52_001178"
     CDS             34667..36736
                     /gene="pknB"
                     /locus_tag="IWL52_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9777754.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
BASE COUNT        12096 a         6501 c         8002 g        10192 t
ORIGIN      
        1 ttttgtgtgc gaatcggtat gctaagaata aaaaagcagg gggcgtttgc ttagtggaat
       61 atcaggtaaa gaaatttgaa gaattgtcaa cagcagagtt ttacgcgatc gtcaaagaac
      121 gaatcgcagt ttttgttgtg gaacaacagt gtccttatca agaagttgat gacattgatg
      181 gacaagcttg gcatacgttt tttcaagata ctgatgggaa gatccttgcc tacacgcgag
      241 tatatgaaga agcggagagt atccattttg gacgtgtcct tgttcatcag gacaaccgta
      301 aagatggttt aggaagaaag attgttcaag aaacgattaa catgatccag aaaaattttc
      361 cagaaaaacc aatcgtgatc ggcgcacaag aatacctaca agctttttat gagtcgtttg
      421 gattcaaggc aatctcagat gtgtatttgg aagatgatat tccacatatc gacatgaaaa
      481 aggaacaagt agcaagttaa gaatctaaaa aaagaaagcc gacactgaaa atcggctttc
      541 ttttttttag attctttatt atttagaagt atcggaaaga tttgtttgct ctactggatg
      601 tgtattgtct tctgccactt gtttggcaag aagatcgcgt atgtcactaa gataatcttc
      661 tgcagtagga gtttcttcca cttccacttg ctcttctttg gagcgtaaat tcttcgcacg
      721 gtttgccgct ttgacaacta agaataagac aaaaccagtg atcagaaatg taatgatagc
      781 actaataacg ttaccaaacg caaatgtgac gccttttgtc ggcgaccagt caagaattga
      841 aagtgctcct tctaaatcag aagtacctgt gatcattgcg acaaaccaac ctattagtgg
      901 tgtgatcaag ccttgcacca cttggtttac gatgcttgta aacgcactac caataacaac
      961 gccgacagca agatccaaga catctccacg catgatgaac tctttgaatt ctttgatcat
     1021 gaatgaccct cccttctttt atttgtcttt agtatatcga aaaataaaaa ataaaaaaac
     1081 aatatacgct tttagccgtc tgtttctttt gataaaaaac atctatgcta tactataaag
     1141 tgtaacatca tttagaaaaa acggcgaatt gcctgaactt ataaatttca agcagaggaa
     1201 taagtaaaaa aggcaggttg acccattaaa agagtaagcg ggcaatcaac tgtgaaaagg
     1261 agcatataaa tatggtgtat caattggcag aaggcgtgaa tcttcatgtc ttaccaacaa
     1321 aacaatacaa aacgatccgg atttttatcc gttttactgc gcgtctgcaa caagaagtca
     1381 tcacgaaacg ttcacttctc agcagcatgt tggaaacaaa tagtttgaat tatccggatc
     1441 agacaaagtt aagtgcgcaa cttgcagagc tttatggcgc aagctttgga ttatcagtta
     1501 gaaaaaaagg aaatttacat tggctaaacg cagggatcag ttttgtgaat ggtgaatatg
     1561 tcaatgatcc aaaccttttt tctcaagcgg tagatttttt aaaagaggtt ttattttatc
     1621 caaatattaa aaatcatcag tttgatcagt tgacgtttga cttggaaaag aacaatctgc
     1681 gtctatattt ggaaagtttg aaggaagata aacaaacttt tgcttcctat gccttgcaag
     1741 aactttattt tgaaaacagc ccggaacaaa agatcccagg gttgggcgta gtcgaagatt
     1801 tggacaagat cactgctagt tcgctagcgt cctactatca agaaatgatg gcaaatgatc
     1861 aaatcgacat ttttgttgtc ggcgatgttg atccggacaa agcagctgaa gcagttgggc
     1921 aattaccatt tgagtcgcgg gaaacagttt atccagatct gttttatacc caacctcaag
     1981 tgaatatcgt gaaggaacga caagtacgtg agccaatcgt tcaagcaaag ctgaacctag
     2041 cttatcagac aaatgtgtat tacgatgaac cagaacgttt tgcgctaatg gtctttaacg
     2101 gtttatttgg cggttttcct cactctaaac ttttcatgaa tgtcagagag aaagaaagtc
     2161 tagcttatta tgcctctagt agcgtggata cgttccgagg attcatgagt gtccaaaccg
     2221 gtatcgatga aaagaaccga aatcaagtac ttcatctgat tcacgagcaa ttagaaagtt
     2281 tgcgaaatgg tgaaataaca gagttggaac tgtctcagac aaaagcaatg ctgagaaacc
     2341 aatatctgct ttctctagac tctccgcaag cggcaatcga agcttctttt ttggacagct
     2401 ggctgccgga aacaaaatta tctgatgaag aatggttgaa acgtatggag agcgtaacga
     2461 tcaaagagat acaacaagta gctgagcaaa tcgaattgca ggcaatcttc tttttagcag
     2521 gaggaaatgc aaatgaataa aacagaatat gagcaaatca atgaaacact ttatcatgaa
     2581 gtactgccaa acggtttgac cgtgtatttg ttgcctaaaa atgactatca caagacatat
     2641 ggtttgttca gtacgaatta tggttcgatc gataacgaat ttattcccta tggtgaaaaa
     2701 gaaaaggtga aagttccaga tggaattgcc cattttttag aacataagtt gtttgaaaaa
     2761 gaagatggtg atgtctttca attatttggg aaacaaggag cttccgccaa tgctttcaca
     2821 agttttacga aaaccagcta tctgttttct acgacggatc aggtagaaaa gaatctgacg
     2881 actctgatcg attttgtaca agcaccttac ttcacagaag aaactgtaaa caaggaaaaa
     2941 ggaatcatcg gtcaggaaat ccaaatgtat gaagacgacc ctaactggcg gatgtttttt
     3001 ggcatactaa ataacttgta tcctacacac ccattgcata tcgatattgc gggcacggtt
     3061 gaaagtatcg acaaaatcac agctcaagac ttgtatacgt gctatcggac gttttatcag
     3121 ccaagcaata tggttttatt tgtagttggt aagatggagc ctgagaaatt gatgaaactt
     3181 attcgggaaa accaagaagc aaaaaatttc cctccaaagc aagaaattgt acgttatttc
     3241 cctgaaaaca caaaagaaat catcaaacaa agtgcattgg aagcagccat tacacgagat
     3301 aaatttgtac ttggtattaa aggattagac acattgccgc aagaaggaac agaactgtta
     3361 cgctataaaa cagcaatcaa tctgttgttc caaatgattt taggcaatac ttctcgaaac
     3421 tatctagcga tgtacaatca aggaattatc gatgacagct ttggttttga attcagcttg
     3481 gatcgtgaat ttcactttgc ggacttcagc ggagatacgg acgaaccaga gaaagcagca
     3541 gaaaaagtca aagaaatcat tcttggattc gctgatgatc cggaagtgtc agaaacaaat
     3601 ctcgatttgc tgaagaagaa aatgcttgga caatatttcc aatcattaaa ttcgatcgaa
     3661 tatattgcca atcaatttac gcaaagttta tttggcgatc gaactttatt tgatcttcct
     3721 gaaatcatcg attcgatcca aatgaaagac gtactggcag ctggggaagc atttataagt
     3781 gaagaggcct tcagcgagtt ttacatgaga ccaaaataaa ccagtatttt atgaggaaaa
     3841 gtcttaaatt tcgttttaga cttgtagtag gaacctcctc caaaatcagc ttttatggta
     3901 ttcttagttg tgttggaaaa attagtgaga aaataaaaca aatcatgctc tcttttaatc
     3961 aacttatatt aaaataaaat caaacgaagt aatcaaagag gttgtgacag aagtggtcag
     4021 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg atatttcggc
     4081 ttattgccga agaagctact tctggaacac cgtgtaccaa gaggttgtga aaaagctgcc
     4141 ctgcatcaag taataagaac agccaagtaa aaacagcttc ttaatgtttt taacttggtt
     4201 ggacttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaatca
     4261 gcgttttgac ctgagtatta gagaaacata cggaaaaata tctccttata ttttgccggt
     4321 ttttgagcta ataccgagga acagctgatt gaacaccgtt taagaaagag gtcgtgagcc
     4381 tgccgtttaa ctccgagtca caaggaacaa gttcaaaaaa cagcttctta tatttattgg
     4441 atattgtgac ttgtgaccga ggagttggca ggagaacaat gtttattcgg aaaaaaggag
     4501 ctgtgacatg acttttgtca cagcacccaa acataacgtc tttcgttgca acagcagatg
     4561 cttcggagaa tcaaacagac tggagaagat gaacgagtgg cttttgaaac catcggagaa
     4621 aaattacggc aagcacgcct gaataaaaaa atatctttag atgaattaca gcagattaca
     4681 aaaattcaaa aacgttattt ggaagcaatc gacaatgatg attttgacca attgcctggt
     4741 aaattttacg tgcgggcatt tattcggcaa tacgctgaag ctgtaggaga agatggagac
     4801 catttagtcg atgtttttga tgggaaaagc aaattgacag gatcggtccc tgaacgtccg
     4861 cagcctgaaa cagtaagtac ttctagaaaa gcaatgcatc aggaagaatc gcatccaagt
     4921 cgcttttgga caagtcttcc tgtcatttta ttaggattgg ttgctttggc gattgtcgtt
     4981 gtcgtgggct atatgacttg gcaagatcgt caagctgatc caataatcgg agacacagct
     5041 tcttcgctta aagtagaagg atcagtcgag gagtcctctt cctcagagca aagcacgcag
     5101 accaaagagt cttctacagt agaatcaaca acaaaatcaa gtgaagaaaa gaaaatggca
     5161 atagctacag gagaagattc tggcagtgcc atcgcagtaa atatcacaga cgctaccaag
     5221 cctgtaaccc ttgaatttac tgctgtcaat cgtgtatggt taggtgtgct agtagacaat
     5281 gcctatgttt atcaagggac actggcagca aatgaaacac agagtaccgt gttaccggaa
     5341 acagcaacaa atgcgaccat cacgattggt gcagcaagca atgcaacgat caaagcgaat
     5401 ggagaatccg taccagttaa tccaggggaa aacaatcaga gtccaaaaaa tgtgaacctt
     5461 actcttcaat acgcagaatg atcgtataaa ttagtttacg catctgttta cttgtaggta
     5521 taaaaaagaa ggatcgtgac aaatctgtgt cgcggtcctt cttcgttaat tcaaaaagat
     5581 caatttcaat aaaaatactc ctttgaataa attcatttgg aaaagtaaat aatatcggtg
     5641 gaaacttcgc tgatagtcac gtagaatagg gaagaaagga gtcgatagtg tgaatttacc
     5701 aaataaatta actgtactta gaatttgtat gatcccgatt ttcattttgg ttgtaggttt
     5761 acctttagat tggggacaat ttaacgtagg agacaccaca ctggctgtca cacagctagt
     5821 aggtgcagtt atttttgctt tagctagtat cacagactgg ttagatggaa aaattgcccg
     5881 tgcgcgaggc cttgtcacta attttggcaa gtttgcggac ccgttggcag ataaaatgct
     5941 agttatgact gcttttatca tgttggttga ccaaggaaag gcccctggtt gggtagtagc
     6001 gatcattgtc tgtcgtgaat tagccgtcac aggcttgcgt ttactacttg tcgaacatgg
     6061 agaagtcatg gctgcagctt ggccgggaaa agtcaagacg gctacacaaa tggttgctat
     6121 tatcttgctg tttatcaata atatcccatt cagcctgatc ggtattccag tagatcaaat
     6181 catgctttat gcttgtctga tctttactat ctattcaggt gtggattact tcatcaaaaa
     6241 cagcggtgtg ttcaaaggat caatgtaagt atcgtttagt ggagaggttg tgagcctgcc
     6301 gtctaactct gagtcacaag ggataatttc aaaaaacagc ttttcatgtt ttatgtatat
     6361 tgtgacttat gaccgaggtt ttggcaggag aacactgttt atttggaaca aggagctgtg
     6421 acaagatttt tgtcacagct ccttgccctc tttttaaata aaaaaatata aaaatacagc
     6481 gtatactata taagggagcg ggaagtaatg aaagcagaaa taatagctgt cggaacagaa
     6541 cttttactag gacaagtcgt gaatacgaat gccactttct tgtcagaaca gctagctgat
     6601 ctaggtatcg aagtttatta tcaaacagtt gtaggagata atcagcagcg tttagaagag
     6661 ttgattgctc tagctgaaac aagaagtgaa ctcatactct tatgtggcgg attaggaccg
     6721 acagaagatg acctgacaaa agaagcaact gcggctcacc tcggtaagtc tctcatacaa
     6781 aatacggaag gatacaaaaa attattggca tattttgaaa caacccatcg aaaaatgacc
     6841 aaaaacaatc tgcaacagtc ccagattatc gaaggcgggg ttcctctacc aaatcgtaca
     6901 gggcttgctt tagggacttt ttatcaaaca gatacacatg cgtatatcct tcttcccggt
     6961 ccgcctaatg agttgaaacc aatgtttgtg gaacaagtac gtcctttact agaggaacgt
     7021 tttccttctg aagaaaaact aatttctaaa gttttgcgct tttatgggat tggtgaatcg
     7081 cgactggtca ctgagctaaa agatctgatt gagacacaga tcaatccaac gatcgcacca
     7141 tatgccaaac caaatgaagt gacattacga ctgacagtga aaacaaatga tgttcaggca
     7201 gggaatcaag ctcttttagc attagaagaa aaaatccaag aacgagtagg ggaatatttt
     7261 tatggatacg gcgatgataa ttcgctagca aaagtcgtag tggaactact gaaagaaaac
     7321 aagcagactg taacagcagc ggaaagtttg acagccggcg ccttccaagc cgcattaggc
     7381 gacattgctg gtgtatctga agtatttcca ggcggattcg ttacctactc gctccaaaca
     7441 aaagctggtt ttctggagat tgatccggag ctattagcag aatatggaac agtcagcaaa
     7501 gaatgtgtgg agcagatggc cattcaagca cgtgagatag caagaacaga ttacagtgta
     7561 gctttcaccg gagtggctgg accggacacg ttagaaggtc aatctgcagg aacagtatgg
     7621 atcgctattt catctaaaga cggcgtggtt tcggaaaaat atcactttgc cagagatcgt
     7681 gctgctatca gatataatgc ggttatgcaa ggatttgatc ttttgagaaa acagctttta
     7741 agaaaatgat aaatgcgaat atttgttcgg ttttttcttg ctttttttca ctaaaaaaga
     7801 tatgataagg ataatgaaat gttgctctga tttgtaaata taaggaggat tatcgtattg
     7861 gcagatgatc gtaaagcagc cttagatgct gcactgaaaa aaattgaaaa gagctatggt
     7921 aaaggctcta ttatgaaatt gggcgaaaaa atcgaccaac aaatctctac aattccaagt
     7981 ggctctcttg cgttagatgt cgctttaggt gtcggcggtt acccccgtgg acgtatcatc
     8041 gaagtatatg gacctgaaag ttcaggtaaa acaacagttg cactacacgc tattgcagaa
     8101 gtacaaaaaa atggcggaac ggccgctttc attgatgctg agcatgcgtt agatccgcaa
     8161 tatgcacaaa aattaggtgt gaatatcgat gaactacttc tttcacagcc tgacacagga
     8221 gaacaaggtc tagagatcgc tgatgcttta gtatcaagtg gggctgtaga tatcgtagta
     8281 gtcgattcag ttgctgcttt agttccacga gcagaaatcg acggcgaaat gggtgactca
     8341 catgtcgggt tacaagcacg tttgatgtct caagcattgc gtaaactctc tggttcgatc
     8401 aacaaaacaa aaacaatcgc tattttcatc aaccaaatcc gtgaaaaagt aggcgtgatg
     8461 tttgggaatc cagaaatcac tcctggagga cgcgcattga aattctacgc aacgatccgt
     8521 ttggaagtac gtcgtgcaga acaattgaaa caaggtacag atattgttgg taaccgtaca
     8581 aagatcaaag tcgtaaaaaa caaagtagca cctcctttca aaatcgctga agtagatgtc
     8641 atgtatggtt tagggatctc acaagaagga gaacttctag acatggcagt agaaaaagac
     8701 attgttgata aaagcggtgc ttggtattct tataaagaag atcggattgg tcaaggacgg
     8761 gaaaacgcta aaatctatat ggctaatcat cctgaaatga tggcagaagt ttctgctcta
     8821 gttagagcag cttatggtat tggtgaagaa gtcgctgtcc cagaagatga aaaaggacaa
     8881 gaagaattgc ctttagtaga agaataaaaa ttatgataaa caaaaacggc tggtctgaag
     8941 agtaggtact ctccttgacc tgccgttttt ttattgaata aagaaatcgt gagttattta
     9001 attatacgag tgggtacata aaataaaaat atctgttcat ctacaaagaa taatttaata
     9061 gaaaagtttc tcaatttaaa ggtttaccta tatgaaacta gtgtcattta ttttaaaaac
     9121 catcgtttaa tgaataagta gaaagccgtt tctttgttta tttaaagttg acactacggg
     9181 gtacaaacat tagaataaag ttgtgcatgt tttttgtaca catatatgcg aagatgcata
     9241 ttgacgagtt tagaataatt tatcatagaa gctgtataga tgataaaaat gaaaaagata
     9301 gtatggaggt ggattttatg gtattcaaca ttctcctcgc tatcatcggt ttaattgtcg
     9361 gtcttggttt agggtttatg gtcgcaaagt cacgtcacga gaaagaaata gctggtgcac
     9421 aatcatcagc tgctggaatc atcgatagtg caaaaaaaga agcagaaact ctaaaaaaag
     9481 aagcattgtt agaagctaag gaagaaaatc agaaataccg ttctgaagtt gaaagtgagc
     9541 tgagagaaag taagttagaa ttaaaatctc aagagaatcg tttgattcaa cgcgaacaaa
     9601 cgctgaatcg taaagatgac tcgttagaaa aacgcgagaa ctcacttgaa agtaaagaag
     9661 aagcacttag ttcaaaacag caattaattg aagagcgaga aaaagacgtt gaaaaaatga
     9721 ttgaagatca acaaaaagaa ctagaaaaaa ttgctggttt atcaaaagag gacgcacgag
     9781 atgtcatcat gaaatcgact gaagaagagc tgaatcatga gttaactcta atggtaaaag
     9841 aatctgaaca gcgtgccaaa gaagaagcag atcgcaaagc gaaaaactta ttatcattag
     9901 cgattcaacg ctgcgcagca gatcaagttt cagaaacgac tgtttcagtc gttacccttc
     9961 cgaatgatga aatgaaaggt cgtatcattg gacgagaagg tcgtaatatt cgaacgcttg
    10021 agacgctgac tggtatcgat ttaatcatcg atgatacacc ggaagcagtt gttctttccg
    10081 gattcgatcc aatccgaaga gaaatcgcac ggatgacctt ggaaaaactg atacaagatg
    10141 gacgaatcca tccagcgcgt atcgaagaaa tggtcgagaa atctcgtaaa gaaatggatg
    10201 aacgtattcg cgaatacggg gaagaggcag cgtttgaagt cggtgctcat acacttcatc
    10261 cagatctaat caagatcatc ggacgtctgc gattcagaac tagttatgga caaaacgtct
    10321 tgaagcattc ggtcgaggtt gcaaaattag ctggtatctt agcagcagag cttggcgaag
    10381 atatccaatt agcaaaacgt gccgggttgc tccatgatat tgggaaagca cttgaccatg
    10441 agattgaagg atctcacgtt gagatcggtg cggaattagc tgccaagtac aaagaaaacc
    10501 cagtagtaat caacgcgatc gcttctcatc atggagatgt ggaagctact tctgttatct
    10561 ctgtccttgt agctgcagca gatgcgctct cagcagcaag acctggtgcg cgaagtgaat
    10621 cattagaaaa ctacatccgt cgtttaagaa gtctggaaaa tatttcgaac agcttcgccg
    10681 gtgtagaatc aagttacgca gttcaagctg gacgcgaagt tcgggtaatg gtcaagccag
    10741 aagaaatctc ggatttagac gctgttcgtt tagtacgtga tattcgtaaa cgaatcgaag
    10801 aagaactgga ttatcctgga cacatcaaag taatcgttat tcgggaaact cgtgcaactg
    10861 attatgctaa ataataaaaa atacaaaaga agagccgcgg caaaagccac agctcttctt
    10921 tttttgtaag gcacgaaaaa acattgctat cattattttt tattcaacat ctcaagatct
    10981 ttctcgatcg tcgtattgat tcctttgatg attgctggga tgaatcctgc ttcttctaag
    11041 gcaactacac ctgcaacagt tgtaccgcca ggagaagaaa cttggtctac aagttcccat
    11101 ggatttttat cacttgaaag gaatttttga caagaaccca taattgcttt tgcagcaatc
    11161 tcagtggctg tctgtttgga caaaccattc agtacaccag tccgtcccat cgcatcgatc
    11221 agcatgtaaa cgaatgcggg tgagctgcca gcaaggccaa tgaatgtact gaaatctgat
    11281 tctgctaaag gatagacgct gccgattgct tcaaaaatca tcgtagcttg ctgaagctgt
    11341 tcctccgtgg cagcttcatt tttgcaaatc gcagaaacgc ctgctcctac gctgacattg
    11401 acattcggca tcacacgtac gatggaggcc tgaggatctt caagcgctgt ttctaaatct
    11461 gttaatgatt ttcctgaagc aatagaaatg aataagactt tctggctata ttctcttagt
    11521 tcttccaata ctttttcaat gatttttggt ttcacggcta aaagaacgat atctgattgt
    11581 gatgcaactt cctgattact tttgcatgca tggacgccag tttcagcagc aaatttttgt
    11641 gcattttcaa aatgtccact ggaaacgaac acctcttctt tcgagactgt tccttttttt
    11701 aataaaccat caataatagc ttgtgccata ttccctgtgc cgataaatcc tactttcatg
    11761 ttatcctctc ctttagcatt aggatatctt tctttttcta tcatcgcaag caaagaacag
    11821 gtctaaagtg gaaaaattaa aataaataag ataacattgg ggtatgacac aaaagtatac
    11881 aagttaggaa tgggaaaatg tgaaaaataa acgatacttg atgctgcaat ggataaagga
    11941 acatagtctg attttaaaac tgattttttt cggctccgtg ttagtctttg tggcaaatca
    12001 agtcgcaaat attgctaatg gcatgagttg gcaagatatt tggcaaagga tgggacaaca
    12061 aagccgcatg acgatcctgc tcatgattct ggctggactg ctgggtgtca ctccgatgtt
    12121 gttgtatgat tgggtgacga tcagagtctt agaaaaacaa ggaaaaccaa aaatggaaag
    12181 aaaagagttt cttcttgctg cgtgggtaac aaacacgatc aacaatctcg ccggttttgg
    12241 tggtgtggta ggagcaagtc tgcgggctag tttttatgga aagcagacaa atagaaaaat
    12301 ggttttagcc actgtatcaa aagtcgcact atttatgctg actggtttgt ctgtatggtc
    12361 gtttcttaca tttattgatg tcttttttat tcagtcagaa agtatttttc gaagctattg
    12421 gatctggctt ttcggcggta gttttttagc accagtcatt ttcttttttg cttatctaaa
    12481 gagaaagaca ttgttcttcg agttttatcc aaaaggcttg cttggtctgc tactggcatc
    12541 ctttggacaa tggacgggag cccttgcggt atttttgatc atcggcaagt tgatgagaat
    12601 agatatttcg atgctttcga tctatcctat gttcatgatt gctacattga taggaatgct
    12661 gacgatggtc ccaggtggaa tggggacttt tgacgttttg atgatcttag gaatgagtca
    12721 attgtctgtt cggcaagatg tagcagtagt ttggctatta tattatcggt tgttttatta
    12781 tgttctacct tttatgacgg gacttctatt gttcatcaca catacaggag tcaaaatcaa
    12841 tcgattttta gataacattc cgcgactgtt tttccaaaag gttgcgcata tcgtgctcgt
    12901 aagtgctgtc tattttgcgg ggatcatgat ggtacttcta tcaacaatca ctaatctgtc
    12961 tgcaatcagt cggctatttc aagtattatt gccgttctca ttcaattttt tagatcagac
    13021 agtgaatcta ttggttggct ttttattact cggccttgct agagcacttt cagtaaaagt
    13081 caaaaaggca tttctgccta cgattgcttt actatctttt ggtattttaa atacaatcac
    13141 acgtacgtta tcttggcagt tgatattggt atatgtcttg attctgttag ctgtgtggtt
    13201 atccagaaaa gaattttatc gaacaaaatt cgtttattct tggggagcta taatctttga
    13261 tgctagcttg ttcggctttt tgtttatcgt ttacgcaatt gcaggctatc acagcggacg
    13321 ctttttagtc ggtcgaataa caggaaatca ttttattttg tttccttcag atgatgtgtg
    13381 gttctcaggg cttattggtt tgggcatctc attgctagct ttagtggcgc tttatcaata
    13441 tttagctgaa gatactacga agcttggcag tgagtacgaa aaagaccgtt ttgaccgttt
    13501 attggaaaca tacggaggca cagctacaag ccatcagctt cgcttgtcag gttacagtta
    13561 tttttattac caagaagacg accaagatca agtcgttttt ggttatcaga tcaaaggaaa
    13621 caaatgcttt gttttaggag atccagttgg caatcctaaa atgatgcggg cagcaacgaa
    13681 tcagtttatg aaacaagcag attgtttagg ctatcagctt gcgttttata agatatcaga
    13741 aagctacgta gtgatgcttc atgaattggg ttttcacttc acgaaagtag gtgaagcagg
    13801 gattgttgat ctagcaacag aagacttgtc tcggctgagt cagcaacttg aatatcaacg
    13861 tttgatgaaa gaaggatacc attttacttg gtacgatcaa ctgccggaag aattacatca
    13921 agctgtaaat gaagtttcac aaaaatggct tcaaggtgca cgagaaaaat acttctctgt
    13981 gggacgattt gataaatcat atgtattatc tagcggtgtc ggtattgtcc gtaaagatca
    14041 aaaagtcgtt ggcttcatca ccgctcagcc gatgacgaag aagcaggcgg gatatgattt
    14101 gttgcgggtc ttacctaaag aatctgagga attagctgac tatctgttag ccaatctgtt
    14161 tattgtatac gagaaaatgg gctactcgga agccaatcta ggcttagctc cattagctaa
    14221 tgttggagaa acagattttt ctttctttca agaaaaagtc atgcatatcg tatataatta
    14281 tggtaatttt ttctattcct tccagtctgc ttatgaaaag aaattaaaat atgtcgatca
    14341 ttgggaagga cgttattttg cttataggaa aggaagtaat ttcggatttg ctaccatgca
    14401 gctactcagt ctgataggaa aagaaaaagg aaatgttccg agtttggcag aagaagtgct
    14461 cacagaatta taagaatcac tagaacttgt ttggattatg aagagaaaat tctgtgaaag
    14521 ctgttgaaac gatgacaaaa tcaaccacta ggcagacata agctatactt ttatttttcc
    14581 ctatgataga atacttgcga cttagacttt tcgaatgggt gagaggatgg aattgattga
    14641 aaaagcgcca gccaagataa atttaggact ggatgtatta tatagacggc aagatgggta
    14701 tcatgagttg gaaatggtga tgtcgagcgt agacttggca gatcatctat tttttgaaga
    14761 aataacagaa gacaaaatca ttgtagaaac aaataaagca tttttaccgg tagatgaacg
    14821 gaacaatgtt tatcaagctg ctttattgct aaaaaataaa tacaatatca aaaccggtgt
    14881 caagatcttt gttaagaaga atattccagt agcagctgga ttaggcggag gaagtacaga
    14941 ctgtgcagct gctctaagag ggatcaacca attatggaat ctgggctgtt cgttagaaga
    15001 actggcagaa atcggtttgg aagtcggtac agatgttcct tactgtgtct acggaagtac
    15061 ggcatttgtc ggtggcaaag gggaagaaat ccaagtatta ccttctatgc cacagtgctg
    15121 ggttgtgttg gtcaaaccaa gaatgagtgt ctctacacgt acagtgttta gagaagtaga
    15181 tacgcaagcc atccaacatc aagacatcca cggacttgct gaagccatcc gagagcaaga
    15241 ttatcagcag atgatcaaaa aaatgggaaa cagtttagaa gatataacgg gaaagcggca
    15301 tccagtcatc catcaaatca aagagcgtat gatcaagtac ggagcagatg ctgcattgat
    15361 gagtggaagc ggaccaaccg tttttgcctt gtgtcaacat tattcaagag cgcagagagt
    15421 atataatggg ttaaaaggtt tttgtgagga agtttattta gtacgaaccc tgcaataaaa
    15481 tataagaagt aaaataggtg atgaaaaact gaggaatcct aaaatggtat tcttcagttt
    15541 tttattgtag tgaaatggct agatatgagc agcaactcat ccattttacg atttaatcaa
    15601 gaatatacat gcttaaatca gaaattgatg gatttctatt gactgtgttt ttttattttg
    15661 atacattagt tcacgttaat aaatcgtaaa attccgattt gttcattgaa aggagcatat
    15721 aaatgaaaaa atatcttttg acagctacgg taatgatagg tgctttactg tttgcagcat
    15781 gtggaaatac gaacaaggaa gcagacaaaa aagaggattt gacgatcgtt acgacttttt
    15841 atccgatgta tgattttaca aaagagatcg taggggatga aggaaacgtg aaacttttga
    15901 tccctgctgg aacggagccg catgatttcg aaccaagtgc aaaagaaaga gcagaaattt
    15961 ccgatgcaga tgttttcgtg tataatagtt cagacatgga attttttgtg gattcgttaa
    16021 aggattcagt ggatagcaaa cagaccttga tgatcgaagc agctaaagga atcgaccgtt
    16081 tagaatcgca agaagcagac gaacatgaag aatcagaaga agggcatggt cattcacatg
    16141 aatacgatcc acatgtttgg ctcgatccgg tattagctat aaaagaagtc agaacgattg
    16201 ctgaagaatt aggcgaaaaa tatccagata aaaaggaaac ttttacgaag aatgccgatg
    16261 cttatatcaa aaagcttgaa gcactggatc aaaaatactc cgatgagcta aaagatgcaa
    16321 caaaccgcac atttgtcact cagcatgctg cttttgctta tttagcgaat caatacgact
    16381 taaaacaagt agcgatttcg ggcgtttcac cagatcagga acccacacca agtcgcttag
    16441 ctgagctaaa agaatttgtg aagaaaaaca acatcaaagt gatttatttt gaagaaaatg
    16501 cctcttcgaa ggtagctgag acattgtcga atgaaacagg agtgaagcta gaagtgttga
    16561 atcctttaga aagcttgact aatgaacaaa tcaaagcagg cgaaaattat atttctgtca
    16621 tggaaaaaaa tctgaaagct ttaaaagaaa gtatcaatta agttttagaa tggaggttgt
    16681 aaggtgcgtt atatcgaaat atctgatttg accttttact atgatgatga acctgtacta
    16741 gaagatgtct cttatcatgt agatgcgggt gaatttgtca ttttaactgg agaaaatgga
    16801 gcagccaagt caaccttggt gaaagcatcg ttaggattac tgaaaccttc aaaaggaatg
    16861 ataaagattg ctgagaaaaa tcaagaagga aaaaaattaa gtatcggtta tattcctcaa
    16921 caagttgcat ctttcaatgc aggttttcct agtacggtga tcgaattagt ccgttcagga
    16981 agatatccaa gaggccgctg gttcaagcgt ctctctgaaa aagatcatca acatgtacag
    17041 cgtgctttag aagctgtagg gatgtgggat atgcgaaatc ggcgaatagg tgaattgtct
    17101 ggcggacaaa agcagcgaat cagtttagcg cgtatttttg ctactgaccc tgacttattt
    17161 gttcttgatg aaccaacaac aggtatggat gaagaatcca gaaatgaatt ttatcgtctt
    17221 ctccgacata gtgcgcatgt ccatgggaaa tcagttctaa tgatcaccca tgatcatgaa
    17281 gatatcaaac agtatgcgga cagacagatc cgactggtga gaaaggagga ttctcaatgg
    17341 cgttgctttc atatgaattc atgagaagag cgtttcttgc cgctatcttc attgccggaa
    17401 tcgccccgat gctaggtgtg tttttagtga tcagaagaca atcgctgatg gctgatacgc
    17461 tttctcatgt ctctttagca ggggtagcgt taggattctt tttgaacctt aatcctacac
    17521 ttacgaccat gctagtcgtt gtattagcag ctgtgatatt ggagtatctg cgaacgatct
    17581 accgaaccta ttcagaaatc tccattgcca ttttaatggc tggtggtttg gcgctagcgt
    17641 tagtattgat gaatttgagc ggagggaatt cagcaacaag tatccaatcc tatctgtttg
    17701 gttcgatcgt gaccattaca tgggagcaag tcatcttttt aggaatctta ttcgttgcca
    17761 tcggtatatt gttcttttta ttcaaacggc cgatgtatgt tctgactttt gatgaagata
    17821 ccgcccacgt ggatgggcta cccgttcatt tgatgtcgat gatgttcaat gtgattactg
    17881 gtgtggctat tgctgtgatg atcccgattg ccggtgcact tttgatatcc gcaatcatgg
    17941 ttcttcccgc agcaatcggt atgcgattag gcaaaagctt caatgtcgtg attgctatca
    18001 gtgtttgtgt aggatttcta gggatgctgt caggactaac tagctcgttt tatctagata
    18061 cacctccagg agcaacaatc acattagtct ttattgcttt gttcctgatt gtaaatatca
    18121 tcaaacgaat attggttgcc ttcagaagaa aaaataacta aattataaag aaaagaagcc
    18181 ttataaaaac gaaaaatttt ttataggctt ctttttattt taataaatac cgaactattt
    18241 tcttattttt tcataaaatg ctcgaatttt tgaagaaaaa cttatataat gtaaaacgat
    18301 aaaatttctt aaaaggagtt ggaataggtg aaagtacgtc gcagtgaacg tctagttgac
    18361 atgacacagt atttattgaa tcacccgcat gaattgattt ccttaacaag ctttgccgaa
    18421 cgttatgaat ctgctaaatc ttctatcagt gaagatttgg gaatcatcaa gaaaacattt
    18481 aaagaacgag ggtatggaac cttggaaaca atcccgggtg ctgcaggtgg tgtattgttc
    18541 atccctgaaa tttcatatga agaggcgaaa aaatatattg aatctctagc agaacgtcta
    18601 tcagaacaag accgtctgct tccaggagga tatgtttatc tctctgattt gttaggagaa
    18661 cctgaactgc tgcgtcaagt tggaagaatc attgcttcta aataccttgg tgaaaaaatc
    18721 gatgctgtaa tgactgtagc cacaaaaggc gtacctattg cccaagctgt gtcctattat
    18781 ctgaatgcgc cattcgttat tgtacgccgg gactcaaaaa taacagaagg atcaacagta
    18841 agtgtcaact atgtgtctgg atcttctgaa agaattgaaa agatggaact gtctaagaga
    18901 agtttgaaac gtggttcacg tgtccttgta gtggatgatt tcatgaaagg cggcggaaca
    18961 gtcaacggaa tgaagagctt gattgaagaa ttcgaatcag aacttgttgg catcactgta
    19021 tttgccgaat caaaattcaa cggtcatcgc gccatcgatg attatacgtc actgctttat
    19081 gtcaaagatg tagatactca aacgaaaaat attacggttg tgccaggaaa ttatttttca
    19141 gaataagagg ttgtgaaaaa gccgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat
    19201 ggctttccac attttttcat aaatttggct taataccgag aaactggctt ttgaacaccg
    19261 tttatctaga ggttgtgaaa tcagcgtcct gtcctgagta ttagctcaat aaacggaaaa
    19321 attgctcctc atattttgcc gtttattgag ctaataccga aggtcagctg attgaaaaac
    19381 gtttaatgaa gaggttgtaa gcctaccgtt taactctaag tcacaagaaa cgatttcaaa
    19441 aaacagcttt tcatgtttta tgagtagtgt gacttgtgac cgaggagttg gcaggagagc
    19501 accggttacc aaatagatag gcccgttatg gtctatctat ttttttatcc agaaaacgct
    19561 gggataaaag aaaatcactt gttttgttga aaaaatgggt atactgggat aaactgaaat
    19621 cagcaaacta tatttaggag tgacgcagtt ggacgcacgt tatgcaatta ttttggcggc
    19681 agggaaaggt acccgcatga agtcaaaatt atataaagtt ttacatcccg tttctggtaa
    19741 acctatggta gaacatatca ttaatcgggt cagtgaaacg aaaccagatg aagtgattac
    19801 gatcgttgga cacggagcag aacaagttaa agcacaacta ggagaacgca gtaaatatgc
    19861 gttacaagca gaacaattgg gaacaggaca tgctgttttg caagctgctt ctttcttgga
    19921 aggaaaaaaa ggaacaacgt tagttatcag tggcgataca ccattattga ctactgaaac
    19981 gctgaacaat ctttttgaat atcaccaagg aaaaaatgca agtgcaacga tcttgacggc
    20041 acaagcagaa aacccaacag gatatggacg aatcatccgt gaccatattg ggatcgtaga
    20101 aaaaatcgtg gaacaaaaag atgcaacacc agaagaagct cttgtacaag aaatcaatac
    20161 aggaacttac tgttttgaca acgaagcttt gttcgatgcg ttaagcaaag ttggaacaaa
    20221 caatgctcaa ggtgaatact atctaacaga tatcatcgaa atcttaaaag aagaaggcca
    20281 tacagttgct gcttatcaaa cggatgattt tgaagaatca atgggagtca atgatcggat
    20341 cgcgttagct aaagcaaatg agatcatgag aaagcgaatt aaccagatgc atatggtcaa
    20401 tggcgtaagc tttgtcgatt cagcaacgac atacatcgat gcaggcgtag aaatcggtcc
    20461 agacacattg atcgaagcag gtgtacaaat ccaaggaaat acagtcattg gttctgattg
    20521 tgtcatcggg tcacattcaa aaatcgtaga cagtaggatt gaggatcacg tcgtcattga
    20581 aaactcagtc attgaaagca gccatgtcaa gaaacatgca gatgtgggac cttatgcaca
    20641 tttacgacca aaagctgaaa ttggcgaaaa cgtccatatc ggaaactttg tggaagtgaa
    20701 aaatgctcaa atcggcaaag gaactaaagt cggtcacttg acttatgttg gtgatgctac
    20761 gctgggagaa gaaatcaatg tcggatgtgg tgttgtcttc gtcaactatg atggaaagaa
    20821 caaacatcat acgactgtcg gcgaccacag ctttattggt tctagcacga atatcatcgg
    20881 acctgtcgag gtagctaaaa acagttcgat tgctgccggg tcaacaatta cagacaatat
    20941 tccagaatat gcgctggcaa ttgcgagagc aagacaagtc aataaagaag gatatgcaaa
    21001 aaaactccct tatctaaact aagacttttg caaaatcact tgatttattt aaaaaatatg
    21061 actactattt attaggtaga attctagagt tgtgaaaaag aaagtggagg tccccatgtc
    21121 aaaacattac tttgacccaa gattaaaaat ttttgcgtta aattccaatc gcccattagc
    21181 agagaaaatt gcagcagcag tcggcgtcga gttaggaaaa tcatctgtta ctcaatttag
    21241 tgatggagaa attcaagtaa acattgaaga aagtattcgt ggatcgcatg tctatgttat
    21301 tcaatcaacg agcagcccag tcaatgataa cttgatggag ttgctgatca tgattgacgc
    21361 actgaaacga gcaagtgcaa aaacaatcaa cgtagtcatg ccttattatg gctatgcgcg
    21421 tcaagaccgt aaagcacgtg cacgggaacc aatcacagcg aaattagtag caaacatgat
    21481 tcaaaaagct ggtgcaacaa gaatgctgac tttggacctt catgcagtcc aaatccaagg
    21541 tttctttgat attccagtcg atcatttgat gggtgcgcca cttattgcca attatttctt
    21601 agaaaaagga atcaaaggcg atgatgttgt cgttgtttca ccagaccatg gtggtgtcac
    21661 acgggcacgt aaattagcgg aatttttgaa atctccgatt gcgatcatag acaaacgccg
    21721 tccaaaagca aatgttgcag aagtaatgaa tatcattgga caagtcaaag gcaaaacttg
    21781 tgtactgatc gatgatatga tcgatacagc aggtacgatc acattggctg ccaatgcttt
    21841 gaaagaagct ggtgcaacta gtgtgtatgc ttcatgtaca catccagtat tatccggccc
    21901 tgctttacaa agaatagaag attcagctat cgagcgtcta gttgttacag actcgatcta
    21961 tcttccagaa gatcgtaaaa tcgacaaaat cgatgaagta agtgttggcg gactaatggg
    22021 agatgcaatc aaacgtatcc atgaaaacaa accagttagt ccattgtttg aaacaaaaaa
    22081 taaataataa ataaactcac aaaggggagt ccaattgggc tgagattgaa tctatttcta
    22141 aacccttcgt acctgtatcg gttatgcgag cgtaggaatt gtgaataaac agctgtttga
    22201 ctattttcga aagctgcttc ctcctttgtg acttttttat tcaaaggagg aagttttttt
    22261 atgggaaaaa atagagtttg ggtagaagga acgattgttg cagcgttagc gatggtgttg
    22321 tcgttgattc cgatccagat cggcagcagt ttttctattt cattagggca gattccattg
    22381 actctttttg cgttaagaag agggacaaag gcagggatgc tggcaggttt catttggggc
    22441 atcctacatt ttccgttggg acaagtttat tatttaagtg tcgttcaagt gttgattgag
    22501 tacccgattg cctatacttt tgcaggcgca gcagggctca tggcttcaaa tgtccaaaaa
    22561 ggctatgtag acgataatac gaaacaaatt agaaaaagca tcgtgtttgg tgcattgatt
    22621 ggcgcacttt ccagatattt ttggcatttt attgcaggag tggttttctg gggagcgtat
    22681 gcactatggg ggatgaattc ttggctcttt tctttcgtaa tgaatggtgc cagcgggttg
    22741 agtacagcta ttgtaacgat catcgtttta ttagggatcc aacaaacgac tccttcgatt
    22801 tttgtgccag ataaacgaca ggagctgtaa ataaaaaaga agcaagaaac ttgagacaga
    22861 atcatgtcct agtttcttgt ttccttttta ttttttgata agaaatagat ctacttcaat
    22921 ggcagataag aaagatcggc atcttctacc aattgccagt gtcgatcggt gacagagtag
    22981 attaaagtag cgatgatttc ctcttctcga taccgggtgc cttttaattt ccaaatataa
    23041 tcttcaatca catagattgg gatacgatat ttaccatcga tcaaaccaac ttgatcaaaa
    23101 gaaaaaatga tcccttcctg catcaaatta cgcatttctt tcaataattc cacactgatt
    23161 ttttcaacag gtgtttcttt tttgaaaaaa gattttttta cattgatctt atctgacaag
    23221 atcgaactta atttactagc aaaaatccta taaaattcat ctaaatagcg ataataaatc
    23281 cgattcagct gatcattaag atcgaagtat acaaaattat tctgtagttt ataaaaaaac
    23341 ggcgaatgaa gatgtgtttt catatgacca aaatataata attctgaaat ttctaaaggt
    23401 gtcaattctt tcagcatcgt taaatcagta aagtcgatcc acttcaattc atcgaatcca
    23461 cgcctgttac gagaagagga aaagaaacgc tgaatatttt ctgtaccacg gattactttc
    23521 agccctgtat gggattcata ttctccagca gaagcagatg gatcaagcaa gagcaagttt
    23581 tgaggaaaat gcacgatact gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca
    23641 gcattacttg tggtatcaat atgtacataa actgactcag tcatcatgtt cctcctctta
    23701 tttcctctta atctctttta ttttacaaca tttgcattaa aaaagcttgt ttcttttatt
    23761 ttaaaattcc attacaaaat cacgttttca ttgaaaacac tctcattttc aattaatttt
    23821 tgtgattttt gtatttttta tttaaaggat gttttatgaa ttgttttctt atggaatcta
    23881 caaagtcgaa tacgagactc atgacctttt tatgacgtac tggtatactt gaagaaaaag
    23941 gagagcgcga tggagataaa aaaagtttac ttttcacaga aaatgtcgct gtattcgcct
    24001 atccagcaag caaaacaacc ccttccattt tttcaaagtc atcaagaaag tagagacgct
    24061 ttttggcgtt ctgcaaaaaa gcaatgtgct tttaaagcag tatcgaaaaa atataccgtc
    24121 tattttacta ttgctaaaag tagaggagat gttattttta ataatctgtt gattgaaggt
    24181 gaaacttttg aatggaaaaa attttttcgg tatatggaag cttgcgctcg tttttttatt
    24241 aaagaaaact gttgtttttt atttcaatct cctctatcag aagaatggtt gcgtattttt
    24301 cataaaaacg gctatcaagg aaccacacaa ttaaataaaa agctggtcta tcatacagca
    24361 cttgttttag gtggaggagg agcacacggt gcttatcaga ttggtgtgtg gcaggcgctg
    24421 aaggaacatg atatcaactt tgagattatc acaggtacct cggtaggtgc gttgaacggc
    24481 gcattgattt tacaaggaga tatgaaaaaa gctgtgaatt tatggaaaaa gctgtcgaca
    24541 agacaagtgc ttgcgttacc agaaatggct gcagttgaag accttcgtga acgcttctat
    24601 cgagaaagac gccaaatgac gaaaacagca ctaattgaag gcggggtttc ttctgctcca
    24661 ttggaaaaga tggtcaaaga caatttggat ttgtccttac tcaaacataa tcccaaaata
    24721 cgtttatata cggtgtctac taaactgcca gaactagcgg aagtagtgac tgatatccaa
    24781 cagacaaaaa cagaagaaat accagattgg atactcgcct ctgcttcatt ttatcccgct
    24841 atggcttatc gaaagatcgg aaaaaataaa tatgcagacg gtgggtatcg caacaaaata
    24901 cctattgata ttgcgattaa caaaggagca acagaggcat ttgtcgtaga tgtgcaaggg
    24961 ccaggcccag cgaaaaaaat tcgtatgcca gacattttta tccattggaa atgtcaaaca
    25021 ttgtggacac taggcagctt tctgcttttt gatagccaaa ggaaccaatt aaatttacaa
    25081 ttaggctatc tggaaatgaa aaaacgatta ggctattact atgggaactg gtatacattt
    25141 cattctgtta aacctgcaag aacatactgg cgaggatttt tgtcttactt aacaaaagaa
    25201 attcagttag aattatcttt tttcaaaagc ataaaatttt ggcaaaaact gcgtaattta
    25261 tataagaaca gggtcgtacc agagacatgc ggattggcga tgctagaact tttagcaaaa
    25321 aaacattttt tactgcccaa tgaaatctat caagtagaca tgatgattca aatgatctgc
    25381 caacaagata taaaaagtat gccggatgat ttattggcac aaattggtca attaagtact
    25441 gaagaatggc gaaggtatcg aaaataccag caaaaaatac aaaatgagcg cacgaaaact
    25501 gtttattttg attatctgct acaaggaaaa cgcaaggatc gtttgcagca tgaattgctt
    25561 aagcaaccag ttgacacatt actgatccta tatttatatt atctcaagga ggaacaacca
    25621 tggcacaaga attttcatat gaaatcgtag aagaaatcgc tatattgtca gaaaacaaca
    25681 aggggtggcg taaagaactg aacctagtca gctggaatgg tcgcccgcca aaattcgatc
    25741 ttcgagattg ggcgccagac catgaaaaaa tggggaaagg cctgacattg acgaacgaag
    25801 agtttgaaca actgcaaaaa gcaatagaaa atatgtaatg aggaaggacc ctgtcgaatg
    25861 aaaagtatga ctggttttgg caaagccatc cgcgaaacaa aagcgtatca gttagatatt
    25921 gaaataaaaa gtgtcaacca gcgttttcta gatgttcaaa tacgaagtcc taaaatcctg
    25981 aactttgttg aaaacgattt tcgtcagctt attaaacagc agttaagccg cggtcgaatc
    26041 gaagtatttg tgaatctagc ttatattggc aaaaacgaaa aacagatcgt catcaactgg
    26101 gaattgattg attcacttat cactcaattg acagacggaa ttcaacaacg atacggtagt
    26161 caaaatcaag tgtcaattag tcggatggcg gaacgcttcg cgctggacga aaacttcgta
    26221 acggtagaag aaaaaccgga agagagcatg gaagaattgg cacagttagc tctagataca
    26281 ttgaaaagcg cacttgattc tattgaggcg agtcgagaaa aagaaggtca agctttagct
    26341 ttagtgttga aaaagaatac tgaagaattc aaagaagtac taaaaaaact cgatagtttt
    26401 gtagaaatct atgaaaagga atttcaaata aggtatcaaa aaaagctgga ggaatttctt
    26461 ggagcgaaag tcgatgagca acggttgttg acggagctag ccatcttgtt ggaacgcgga
    26521 gatattcacg aggaactgga tcgtctggcg atccatgtac aaaaactaga tgaacttatc
    26581 gaagcacaat gtcctgtagg tcgggaatta gatttcctga tccaagaaat gaatcgggaa
    26641 atcaacacga tcggttccaa atcaaatgca atcgaaatta aaaatcaagt cgtccagtct
    26701 aagacgatct tagaaaaaat cagagaacaa gtacaaaata ttgaatgacg aaagaggtag
    26761 agataatgaa atattatgac gtaacgttcc acgaattaag tggaaaaact gtggttaaac
    26821 gggatatccc ttctgaaaaa aacggctttg acgtatggaa agatgcctgt gcagactata
    26881 atgagaacga actgtttatt ttgatcaacg acggtgctta cgtgacgatg aatcggaaat
    26941 ttattgttcg aattgacaca gaagaggtag aagatccgac agaaaaagca cgttctcgaa
    27001 aagatgagat catgggtgta gtgaatactt tgtccaatat gggcttttag caaggatgct
    27061 gaacaaagaa atacaataaa taatcagaat tggatttctt cgcttagctt ctttgctcta
    27121 ggaatacatt taaaggttgt gacaaaattc gtgtcacaat ctttttttat tttgaataag
    27181 tgatgttcaa aaaccatttt ctcggtatta agccaaattt atgcaaaaat gtgagaaact
    27241 attttcacaa aatttttctt catactcgaa actaatcggc ttcttcacga cctcttatac
    27301 acggcgttca aaagcttgca tctgcgataa taaccccaac caaacgaaaa acatgagaag
    27361 ctgtttatgt ttggctgttc ttattacttg atgcaggaca gcttttttac gacctcttgg
    27421 tacagggtag cttttttacc aatttctagc tataattaaa gataaccttg aaagttgcag
    27481 aaaaaaagtg caaaataaac gataagtaca aaaagactag ttgaaaggac gtcgtcatgt
    27541 cagagcgtgg attattaatc gtgttgtctg gtccatccgg tgtaggaaaa ggcacagtac
    27601 gaaaagcaat ttttgagagt gaggataatg attttcagta ttcgatctca atgactaccc
    27661 gtaaaatgcg tgaaggggag gtcgaaggcg ttgattattt cttccgaagc aaagaagagt
    27721 ttgaatcaat gatcgaagct ggagagatgc tggaatacgc agaatatgtc gggaattatt
    27781 atggaactcc tttgtcatat gtcaacaaga cattggatga aggaaaagat gttttcttgg
    27841 agattgaagt ccaaggagca aaacaagtca aagaaaaagt gcccgatggt gtattcatct
    27901 ttttgactcc gccagattta gcggaattaa aatcacggat cgtaggccga ggaacagatg
    27961 cagatgaagt aatcgaagaa cgaatgaaag ttgcaaaaga agaaatcgaa atgatggctc
    28021 tttacgacta tgctgttgta aatgatgaag tgccgctagc agttgaacga atcaaaaaca
    28081 ttattgccag tgaacatttt cgagtagatc gcgtaatcgg taaatatatt aaaatgttaa
    28141 aggagatgta gtcttatgat gttaaaacct tcaattgact cactattaga tcgtgtaaat
    28201 tcaaaatatt ctctagtgat tttagcaagt aaacgcgcgc atgaattgga tgcaagtgca
    28261 cagcctactc tagattcttt tgattcagta aaaagcgtcg gtcaagcttt agaagaaatc
    28321 gatgcaggaa atgtagtcaa tgatccacat cctgaattaa aacgtgaacg tttgaagatg
    28381 gaagaagagg aacgtcaagc acaaaaagag cgtgaacaac acgaattgga atcacgtatc
    28441 cgtgaagagc aaaaaatgta ataaagaaaa atagagcgaa catctaaaaa gaaaagcaaa
    28501 cccagctgtt tccttttcaa acattcgcca cttattgtct ggtagaaaag gctgtgtctg
    28561 aactttttga cacagttctt ttttttctta tctgctgtta attttttgtt aggatttggc
    28621 agaagataag acaaatccat cgatctctag taaaataggc aaggaacttt ttgtcgcttg
    28681 ataagaaaag acaaaaaaag gaggaaacga accatgacat taacagctga agtcattgtg
    28741 gacgtaccta ctatgcagac cgatcagcca ttcacttatt tggttccctc tgaaatggaa
    28801 acagctttgc aagtcgggat gcgagtagaa gtcccttttg gtaatggcaa tcgtcatgtc
    28861 caaggatttg ttatggctat ccaaaaatca gaagaatcag ctaatccatc tttgaaggca
    28921 gttattcgtt tgctagacct tgctccggtg gtcaatgaag aattactttc attagctgat
    28981 tatatgaaaa aaattactta cgcattcaaa atcacttgtc tacaaacgat gcttccgagt
    29041 gtgatgaaag cagaatatga taaattgatc tatcctttgg cagacacacc agaagttcag
    29101 gtattgtttg atcagcgaga agtgatttcc tggaaggaag cagaagaaga aggcagcctt
    29161 tctcaattga ttcgctggcg ccaagagcaa ttagtagata tcaaatatga agtccataca
    29221 cgaaataaag taaaaacgat tcgtttggtc cgttctttgt taactgaaaa gcaaatcgaa
    29281 gaagaatggg caaaattacg gcaaaatgcc aagaaacaaa aagaacttct tttgtgttta
    29341 tctgaactga gccaagaaga accaattgct tatttcaaaa ataaaggaat cagtactgct
    29401 gtcttgaatc aaggaaaaga aaagggctgg ctggagttta tagaatcaga acgttatcgc
    29461 gacccttata aagaccgagt atttgaccaa acgacggcat tggaactgaa tgcagaacaa
    29521 aaaaatgctg tcgaacaaat catcacagca ggtcagcagc aaaaagatca ggtctttttg
    29581 ctggagggaa tcactggcag cgggaaaaca gaagtatatc ttcaggcaat cgctgatgtt
    29641 ttgtcagaaa ataaaacggc tattatgtta gtgcctgaaa tcgcattgac tccgcaaatg
    29701 gtcgagcgat tcaaaggacg cttcggagaa tcagttgcgg tcttacatag cggactttcc
    29761 caaggtgaaa aatatgatga atggcgcaag attgagcggg aagaagcgca agtagtagta
    29821 ggcgcacgat ctgctatttt tgccccgctg aaaaatatcg ggctgatcat tatcgatgaa
    29881 gaacatgaat caagttacaa acaagatgag acaccgcgtt atcatgcgag agatctagca
    29941 atttggcgca gtaaatatca tcattgccca atagttctag gaagcgcgac gccctcttta
    30001 gaatcaagag cacgagctca aaaaggcgtt tatcagttat tgcagttaaa ccaccgagcc
    30061 aaagcagctg cacagcttcc agctattgag atcgtagata tgcgtgaaga gtttcagaat
    30121 catcggacct ctactttttc tgctaatttg caggaaaaaa tacaaaatcg attagacaaa
    30181 aaagaacaaa ctgtcctact gctcaatcgt cgaggatatt cgtcttttgt gatgtgtagg
    30241 gactgtggat ttgttcttcc atgtcctaat tgcgatatct ctcttacact gcatatggat
    30301 acacgttcaa tgcgctgcca ttattgcgga catgaagaac ctattcccaa tcgctgtcct
    30361 aattgtggcg gcaataaaat ccgctactat ggaacaggta ctcaaaaagt agaagaggaa
    30421 ctccgagaac tttatccgca agctaggatt ttgcgtatgg atgtagatac cactcgaaga
    30481 aaaggggcac acgaacagat tttacaaaaa ttcggtgcaa aagaagcaga tatattatta
    30541 gggacacaaa tgattgctaa aggtttggac tttccagaag tgacattagt tggggtgttg
    30601 aatgctgata cctccctgaa tttaccggat tttcgttcta gtgagcatac attccaattg
    30661 cttacccaag tttcaggacg agcagggcga gcggaaaaag caggcgaagt cgtgatccaa
    30721 acattcaatc ctcaacatta cgccatagaa ttggctaaaa aacaaaatta tgagcagttt
    30781 tatcagcagg aaatgcatgt acggcatcgt ggaggctatc ctccttatta ctttactgtg
    30841 aaaataaccg ccagccatcc tgaagaacaa gtagcggcaa agaaaatctt tcagattgcc
    30901 aatcagctaa aagaagtgtt aagccctcaa agcttgctat tagggccaac gcccagcatg
    30961 attttgagag tgaaaaaccg atattattat caactaatca tcaaatataa acatgaaccg
    31021 aatcttcctc atgttttgga tgaaattttg aatggaagcc agaaagaaca gcgacaaggt
    31081 ctttttgtgg caattgacaa cgaaccatta aactttattt aagaaagggc caaattatgc
    31141 gctatcctat attgatccac cctaatgata aattaaaaag aactgctcag ccaatcgatg
    31201 taatcacgga cgagacaatc gctttattag acaatttata tgagacaatg attgcgaatg
    31261 acggaatagg gatcgctgct cctcaagtag gacaaaacaa gagaattgct gtgatcgaag
    31321 tagatgaagg cgagaaattt gaactgatta acccagaaat catcgaagca aaaggtgaaa
    31381 gccttgacgt agaaggttgt ctcagtattc cgcatgtcta tggaacggtc aagcgagcag
    31441 atgaagtgac cgtgcgttat tatgatcgag acggagaaga gatagaagtg acggcttttg
    31501 gctatttagc ccgtgccttc cagcatgaaa tcgatcactt agacggtatc ttgttcattg
    31561 agaaaatgat acagcagatc ccggaagaag aacttgaaga atatatggag gaacatcttg
    31621 atgactaagc tagtatttat gggcacacct gctttttctg tgccgattct tgaagggctg
    31681 atagaatcag gttacgagat ccaggcagtg gtaacacagc ccgaccgtcc agttgggcgg
    31741 aaacgagtaa ttacgccgac accggtaaaa gaagcggctc tgaagcacgg tatccgtgtg
    31801 ctgcagccag agaaaatctc cggttcgcca gaaatggaag aaatcattga attggtgcct
    31861 gatgtgatcg tcactgcggc atttggccaa ttcttgcctg aaaagttatt gcaagtacca
    31921 aaattagggg caatcaatgt ccatgcttct ttacttccta aatatcgggg aggtgcgcct
    31981 gttcattatg ccatcatgaa tggggagaaa gaaactggtg taacgatcat ggaaatgatc
    32041 aaaaaaatgg atgcaggcgg aatctatgcg caagaaagca taccaatcac taaacaagat
    32101 gatgtaggga cgatgtttga aaagcttagc cttttaggaa gaaagctatt gctagaaaca
    32161 ttgcccaata ttttggatgg acaaaaacca gtacctcaag acgagtcaga agcaactttt
    32221 tcgccaaaca tcacacggga acaagaagca atcgattggc ataaaactgc agaagaaatt
    32281 gacaaccaag tacgcggaat gcgtccttgg ccaattgcat tcacgaccta tgagggcata
    32341 cgctggaaat tattatcggt tgaagtattg gcagaaacta cagacaaagc acctggtacg
    32401 atcatccgga aagacaaaaa aaatctatgg attgcttgcg gtaaacagac cgtcttagcc
    32461 atcaatgaat tacagccagc tggtaaagga aaacaggccg tacatgattt ccttaacgga
    32521 agcggtcagc aagttcagat tggtcagcag gtgaaataat gaaaaaaatc cctaaaaaaa
    32581 tgaaaaaatc agtacgatat atggcattac tgacattaga gcgagtgaac aagggtggcg
    32641 cctactcgaa tcttttgttg aatgagatga tcaaacaaag cgagctaaat caaaaagatg
    32701 tgggtttatt tacagaattg atctatggta cgattagcca tcagcgacta ttggaatttt
    32761 acgttgcacc attgatcgct aaagcaaaaa aagtggatga ctgggtcaag acgcttcttt
    32821 atctttctgt ataccaatta gaatttttag ataaagtccc tgcacatgct attttaaacg
    32881 aggcggtcga aatcgcaaaa gtaaaaggaa atcctgggac agggaaattt gtaaatggtg
    32941 tcttgaggaa ttatcagcgt caaggagcac cagatctgca aggcattgct gatcctatcg
    33001 aaagactttc tgtcaaaatc agcctgcctc tatggatgac taaacgactg gtggatcaaa
    33061 tcggatacga agaaactgaa aagctcggtt tatcgctata tcagcctagc catgctagtg
    33121 cccgagtaga tacacgccga ctttccagag atcaggccat cactgttttg caagaagaag
    33181 agatcacagc tgaaaaaagc caagtcagtc catacggtat agtagcgaaa aaagggcatt
    33241 tagcttctag ctcattgttc cacgatggcg tcatgacgat ccaagatgaa agttcgatgc
    33301 ttgtagcgcc gtctatgcaa atcgagcctg aacatcatgt cttagatgcc tgtgcagcac
    33361 ccggaggtaa gacgacgcat attgcttctt ttttagatcc gtcatgcggt ggagaagtgg
    33421 tcgctttaga tgtacacgaa cacaaagtca aactcattga agataatgca gcacgtctag
    33481 gtgtctcttc tgctgtctac gctcaaaaga tggatgctag agaagtcagt aaacagttcg
    33541 aagcagagca gtttgatcgg attttagtag acgcgccttg ttcaggtctt gggctgcttc
    33601 ggcgaaaacc agatattcgt tataaaaaac agccggatga attagcgaat ttaccaaaaa
    33661 tccaattgga aattttagaa agctgcgcac caactttaaa gtcttccggt attctgactt
    33721 atagcacttg tacgatctta aaagaagaaa accaagacgt tgtttctgct ttcttggaaa
    33781 gacaccctga ttttgagagg attgatgttt tgaccaatcc tatgctacaa tcatcaatcg
    33841 aagagaaaat gttgactttg tatccgcatc agttttacac agatggattt ttcatttgtt
    33901 gtttgcgcaa aaagtaatga ggtgaaaaaa tgcagattga atatcaatca gatgtaggaa
    33961 gacgacgtaa cacaaatcaa gactatgcca gtgtatttac cagccgttca ggtctcaagc
    34021 ttgctgtatt agcagacggg atgggtggtc atcgtgccgg agacattgcc agtcagatgg
    34081 cggtaacgaa tctaggcgaa gcttgggaaa aacaagaagc tgttgacgat gaaaaaatcg
    34141 cccaatggtt cattcaatcg attcaagaag aaaatatgct gatttatcag cgtggacaag
    34201 aacagccgga atataacggt atgggaacaa caatcgtagc ggccgctctg tcagatgact
    34261 ggttcacgat tgccaatgtt ggggatagca gagcttattt gattcgtgaa gataaaatca
    34321 tgcaattgac ggaggatcat tctttagtca atgaactagt caaatcagga gaaatcagcg
    34381 aagaaatggc agtgaaccat ccaaggaaaa atattttaac acgttctgtc ggcatgcctg
    34441 gaacagtcga agtagatgtt tctactcatc tttggcaact aaaagatcga ctgctgttat
    34501 gttcagatgg cttgacgaac atgctgtctg aagaagaaat aggaacgatc atcaatcaag
    34561 aaggcccatt gtctcataaa gtaactgagc tgattgatcg tgccaatgaa gcaggcggag
    34621 cggataatat caccgttctt cttatcgaat ataaggagga cagtgtatga tcgagttggg
    34681 caaaaaatta aatggccgtt atcaaatcac tggaaatatc ggtagcggtg gtatggcaaa
    34741 tgtatttttg gcgcacgatt taatcttgga tcgtgatgta gcagttaaag tcttacgatt
    34801 tgactttcaa aatgaccaga ctgcgattcg acgatttcag agagaagctt tagcagcaac
    34861 agaactcgtt catccaaaca ttgttagtgt ttacgatgtt ggtgaagaag acaacatgca
    34921 gtatctggtg atggaatacg tcaaaggaat ggacttgaaa cgatatatcc aaacacatta
    34981 tcctgttcct tatgaaacag cagtaaatat tatgcagcag atcctttctg ctatttcttt
    35041 agctcataac catcaaatca tccaccgaga tttgaaaccc caaaacgtac tgatcgataa
    35101 tgaaggtgtt gtaaaaatca cggatttcgg gattgctatc gctttatctg aaacatcgat
    35161 cacgcagacg aatacgatgc taggatctgt tcattatctg tcccctgagc aggccagagg
    35221 aagtatggca acgaagcagt cagatattta tgctttaggg atcatccttt atgagatgtt
    35281 gacgggtagc gtaccgtttg atggtgaatc tgctgtaaca attgctttaa aacattttca
    35341 agatgatctt ccatctataa aagcacttga tccaaatgtg ccacaagcac tggaaaacgt
    35401 gattttacgg gcaacggcta aagaacctgc agaccgttat aagtcagccg aggaaatgtc
    35461 tgatgatctt tctacagtat tatcaccagc tcgtgcaaac gaagaaaaat ggcagcctca
    35521 tgtgatggat aatgaaacaa aagttatcac accccttacg gaagatgcac caatgcctga
    35581 ttcattcaaa tctatgcctc tgccaaaaga caagccagaa aacgaaacct taccagagga
    35641 ggaagaagtt ctgcctgatc ctaaaaagaa aggacagaaa aaatggtggc tgatcctatt
    35701 agctattctt gctgttttag gaatcggaac agctgtattc tttgcttctg gcgggcgagg
    35761 agaagttgtt gttcctgatg tttctgatct ttctgaagct tctgctagag aaacactcgc
    35821 aaaagcgaaa ttagaagtag caacaaaaac agaagaaatt gcagatgaca aaatcgaaga
    35881 aggtaatgta gtgaagacag acccggcagc gggaacgaca gtcaagcaaa aacgcgaagt
    35941 cactctgtat attagtagcg gcacgaaaaa aatcaaatta gatgattaca gtggtaaaag
    36001 ttatgaagat gccgaagcaa gtttgaaaga attagggttt tctgctgact tgattcaaaa
    36061 agaagaggaa ttcagttcag aggtagaaag cggaatgatc atttcgcaat ctccatcaga
    36121 agatacagaa gtcgatccta aaacagacac gattactttc acagtcagca aaggaccaga
    36181 aacagtgact ttgagtagtc atgtaggaga aaactacgaa actgttcgta attggcttgt
    36241 tacacaagga ttcaatatca ataatattag tgctacttat gactatagcg acactgtacc
    36301 atcaggttcg atcatcagtc aggatccagc ctcgggtgaa gttgtagcag aagaaactta
    36361 catcaatttt gtcgtaagcc aaggaacaga gcctaaattg aatgatatct ccggctatac
    36421 aaaaagcgaa gcacaaagtt atttagctag tgtcggtgca gaatatatcg ggcatgagac
    36481 ttccgaatat tcaaacacgg tggataaaga taaagtgatt cgtacagtac caggggcagg
    36541 aacgacaatt accaaaggaa tggttgtgaa cgtaatttac tctaaaggac cagatccagc
    36601 gtcttctagt tctgaaaaag actcttcaga cagttctagc agctcaagta gctccagtag
    36661 cacaagcaac tcttcaaaag aagaatcttc cagctccaca agttcatcag ctagcacctc
    36721 agaaagttca aaataagtgc tgaataaaat atatcagaag ggaatgcgac attgatttgt
    36781 tgcattctct t
//
LOCUS       DACDVT010000024        36205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDVT010000024 DACDVT010000000
VERSION     DACDVT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.024"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(69..197)
                     /locus_tag="IWL52_001179"
     CDS             complement(69..197)
                     /locus_tag="IWL52_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9777755.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(200..1573)
                     /gene="rlmD"
                     /locus_tag="IWL52_001180"
     CDS             complement(200..1573)
                     /gene="rlmD"
                     /locus_tag="IWL52_001180"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9777756.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1684..2739)
                     /locus_tag="IWL52_001181"
     CDS             complement(1684..2739)
                     /locus_tag="IWL52_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9777757.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2749..4179)
                     /gene="gatB"
                     /locus_tag="IWL52_001182"
     CDS             complement(2749..4179)
                     /gene="gatB"
                     /locus_tag="IWL52_001182"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9777758.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4176..5648)
                     /gene="gatA"
                     /locus_tag="IWL52_001183"
     CDS             complement(4176..5648)
                     /gene="gatA"
                     /locus_tag="IWL52_001183"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9777759.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5648..5953)
                     /gene="gatC"
                     /locus_tag="IWL52_001184"
     CDS             complement(5648..5953)
                     /gene="gatC"
                     /locus_tag="IWL52_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9777760.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6059..8095)
                     /gene="ligA"
                     /locus_tag="IWL52_001185"
     CDS             complement(6059..8095)
                     /gene="ligA"
                     /locus_tag="IWL52_001185"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9777761.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8112..10346)
                     /gene="pcrA"
                     /locus_tag="IWL52_001186"
     CDS             complement(8112..10346)
                     /gene="pcrA"
                     /locus_tag="IWL52_001186"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9777762.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10501..12060
                     /locus_tag="IWL52_001187"
     CDS             10501..12060
                     /locus_tag="IWL52_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9777763.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12171..14102)
                     /locus_tag="IWL52_001188"
     CDS             complement(12171..14102)
                     /locus_tag="IWL52_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9777764.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14120..15034)
                     /gene="pfkB"
                     /locus_tag="IWL52_001189"
     CDS             complement(14120..15034)
                     /gene="pfkB"
                     /locus_tag="IWL52_001189"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9777765.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15031..15783)
                     /locus_tag="IWL52_001190"
     CDS             complement(15031..15783)
                     /locus_tag="IWL52_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9777766.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15942..16151)
                     /locus_tag="IWL52_001191"
     CDS             complement(15942..16151)
                     /locus_tag="IWL52_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777767.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16162..17490)
                     /locus_tag="IWL52_001192"
     CDS             complement(16162..17490)
                     /locus_tag="IWL52_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777768.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17855..18442)
                     /locus_tag="IWL52_001193"
     CDS             complement(17855..18442)
                     /locus_tag="IWL52_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9777769.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18525..19775)
                     /gene="clpX"
                     /locus_tag="IWL52_001194"
     CDS             complement(18525..19775)
                     /gene="clpX"
                     /locus_tag="IWL52_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9777770.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20022..21311)
                     /locus_tag="IWL52_001195"
     CDS             complement(20022..21311)
                     /locus_tag="IWL52_001195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9777771.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21465..22397)
                     /locus_tag="IWL52_001196"
     CDS             complement(21465..22397)
                     /locus_tag="IWL52_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777772.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22554..23213)
                     /locus_tag="IWL52_001197"
     CDS             complement(22554..23213)
                     /locus_tag="IWL52_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9777773.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23216..24241)
                     /gene="sppA"
                     /locus_tag="IWL52_001198"
     CDS             complement(23216..24241)
                     /gene="sppA"
                     /locus_tag="IWL52_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9777774.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELALNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKFDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24254..24544)
                     /locus_tag="IWL52_001199"
     CDS             complement(24254..24544)
                     /locus_tag="IWL52_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777775.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24954..26759
                     /gene="glmS"
                     /locus_tag="IWL52_001200"
     CDS             24954..26759
                     /gene="glmS"
                     /locus_tag="IWL52_001200"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9777776.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26831..28186)
                     /locus_tag="IWL52_001201"
     CDS             complement(26831..28186)
                     /locus_tag="IWL52_001201"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9777777.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28210..29379)
                     /locus_tag="IWL52_001202"
     CDS             complement(28210..29379)
                     /locus_tag="IWL52_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777778.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29376..30263)
                     /locus_tag="IWL52_001203"
     CDS             complement(29376..30263)
                     /locus_tag="IWL52_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9777779.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30452..34132)
                     /gene="nifJ"
                     /locus_tag="IWL52_001204"
     CDS             complement(30452..34132)
                     /gene="nifJ"
                     /locus_tag="IWL52_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9777780.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34330..34851
                     /locus_tag="IWL52_001205"
     CDS             34330..34851
                     /locus_tag="IWL52_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9777781.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35550..36115)
                     /locus_tag="IWL52_001206"
                     /pseudo
     CDS             complement(35550..36115)
                     /locus_tag="IWL52_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9821 a         7875 c         6433 g        12076 t
ORIGIN      
        1 aaagagccac taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt
       61 tagcacgtct atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta
      121 ggtgtttcca tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt
      181 ttttcttttt ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat
      241 gggtttgcgg gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa
      301 ataacttagc atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg
      361 gtgacatttc gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa
      421 ctacgatcac agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt
      481 ttccgacttc ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt
      541 caattgcttc tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca
      601 agccaatcgt acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg
      661 caaactcgat ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg
      721 actttgctga gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat
      781 ataaaacttt ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga
      841 tgaccgaaac cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa
      901 agaatttttc tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac
      961 ggataatgat gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga
     1021 accgttgcaa gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat
     1081 aaaaatcttc gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact
     1141 gtccatcgat acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca
     1201 ttccgaccgt tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct
     1261 ctacttgttt ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga
     1321 tacctatgcg aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa
     1381 atgtttcgac ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt
     1441 cttctccagg gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca
     1501 ttccttcata actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt
     1561 gctgttctgt catattttgc tcctttaagc ctatttttca atcctactga actctactat
     1621 aaatcagcta attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt
     1681 tgattaattc ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc
     1741 atccgttaaa cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc
     1801 atctgtacct gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg
     1861 ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat
     1921 cattactggt cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata
     1981 gattattctt ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa
     2041 gatctcaaag atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt
     2101 ggcatctggc gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat
     2161 cgaagcattt ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac
     2221 tcgagggagc atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag
     2281 ttctgatgga acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa
     2341 gtaactgtta tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc
     2401 tgctgctgct ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt
     2461 accagctggg atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac
     2521 ttcgttgatt gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac
     2581 tctacatgct tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc
     2641 aaatcctgct tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt
     2701 tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc
     2761 taattcatct ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt
     2821 catgatttga ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac
     2881 agattgttgg ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga
     2941 taattgcacc caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa
     3001 ttcacggaag acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat
     3061 cattcctgct aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt
     3121 caagtaagca gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt
     3181 ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc
     3241 atactctgga agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg
     3301 caaactagct tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg
     3361 ctctgggaag taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt
     3421 cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt
     3481 tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt
     3541 tttcaattct gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc
     3601 atcacagcgc atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat
     3661 tgatcgcaat gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga
     3721 aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat
     3781 accatgcatg ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt
     3841 ctttttacct tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg
     3901 ggaaatttga tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt
     3961 atctttagag atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc
     4021 tttattcatt actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt
     4081 tggttctgca ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc
     4141 gacgtggact tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata
     4201 gctggttgtt gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc
     4261 gttttttcat caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa
     4321 cctgccggta cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca
     4381 ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt
     4441 gaagatggcc cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc
     4501 aatgtacgga cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag
     4561 aatgtaccta acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg
     4621 acatatacat catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg
     4681 aatcgttgca agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg
     4741 tattttgaat gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct
     4801 gctgctttca atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta
     4861 ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact
     4921 gaaacgcctg aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat
     4981 gcattatctt tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca
     5041 atcaaaccaa aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa
     5101 gctgctggtt ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca
     5161 gctgcaactg ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa
     5221 gcattttttg ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc
     5281 atgtttagct taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca
     5341 tcataaattg gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt
     5401 gttacgatat tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc
     5461 cctttttcat ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat
     5521 gcttcgacct caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt
     5581 acagtgattt ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat
     5641 tctgtcatta tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt
     5701 cttctgtttc cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat
     5761 cttgtcgcat cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat
     5821 ctacttcttc cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg
     5881 caagttcttc atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt
     5941 cactaatcgc catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta
     6001 acacaagagt gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc
     6061 attcttctac tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt
     6121 cgattcctaa ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga
     6181 tatccgtttt tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt
     6241 ttgcttcttc acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa
     6301 aaggagactc gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc
     6361 ctgcttgttt taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta
     6421 cactgtcggc aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt
     6481 tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga
     6541 catgtcggat tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga
     6601 ttgctgtcaa aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt
     6661 cttctgtcaa tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt
     6721 ctagtacgcg cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca
     6781 aaccttcttt catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat
     6841 ccaaatggac aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc
     6901 tgtctttagg tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt
     6961 tataaatcag aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat
     7021 gcaggctggc acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg
     7081 gcgttactac ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt
     7141 cttctggcgg aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt
     7201 gatcttgtag cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat
     7261 ccacacgttt gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata
     7321 gacgtttttc ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact
     7381 gggtttttgc ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc
     7441 ttttcgctgt gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat
     7501 tcgcaaagat atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt
     7561 ttggcatgaa acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga
     7621 ccgaccggac agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag
     7681 tcgcacctct cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt
     7741 ttagttcaca gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag
     7801 ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga
     7861 ctttttcaaa accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga
     7921 tcaaatcagg atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat
     7981 agtcttccac tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg
     8041 tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt
     8101 accctccact actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct
     8161 tgacttggga aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact
     8221 gttcctagac cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc
     8281 cctccagtag cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct
     8341 gtttttttag cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt
     8401 aactcctgat cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat
     8461 aatgttctgg agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca
     8521 tatgcgagac ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga
     8581 aaaaccccct cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca
     8641 tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca
     8701 agtgccagat catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca
     8761 ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt
     8821 tcgatgcgtg cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta
     8881 cgatccaaaa cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact
     8941 tcttggatca tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc
     9001 agatccacat tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt
     9061 agtttttcga tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa
     9121 ctgattgaat cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct
     9181 ttacgatcgt agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga
     9241 gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa
     9301 ttccgatgat tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct
     9361 cgttcattat ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag
     9421 ttttttggtt ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt
     9481 ttcgtggagc gatagttttg ttctagcaaa attactgcag catctggata atctttttca
     9541 aaatccaaga tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct
     9601 cccacgacgc acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg
     9661 tgatttgtat cttggtactc atctacatga atataatgga atttgttttg ataatatgtg
     9721 agtgaatcag gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc
     9781 atacattgat tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct
     9841 tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct
     9901 tgagagattg ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct
     9961 tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg
    10021 ttatacccga taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc
    10081 gatacccaga catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct
    10141 tttgcagcct tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc
    10201 tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc
    10261 attagcagta atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg
    10321 tttaataatt cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat
    10381 tatagcattt tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat
    10441 ggatttttcc ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga
    10501 atggaaaaaa aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa
    10561 ggaatatttg ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa
    10621 aagctaagcg atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta
    10681 attggcctag ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat
    10741 gaaccggata tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta
    10801 tcaatgaact ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt
    10861 gcgggccttt ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa
    10921 ggagctggac aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt
    10981 gcaagtagcg gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta
    11041 gaagaaatcc atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc
    11101 actgctaact tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac
    11161 cttccttctt tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc
    11221 atcttaggtt ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg
    11281 cctttaccag cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat
    11341 ttatggccta gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc
    11401 ttgccaatca ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct
    11461 tacggtggtg gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc
    11521 ggtagctttt atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat
    11581 tttgtcgtta ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca
    11641 attattttag tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct
    11701 gctcttgttg cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta
    11761 ttggaacgtc tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt
    11821 gattttccag taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg
    11881 cctaaaaata tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt
    11941 gatacgatca tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca
    12001 agaatcaagc aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa
    12061 cgaacatgga aaaactattt tacaacaact attcatctac actacttgat tctatatgat
    12121 tctataaaaa aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct
    12181 gctgctgcag gagcttttct caataaacca taaatcacac cagatacgac tgctccgata
    12241 gcaataaaca ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca
    12301 tgaggagcca gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca
    12361 ataataaaac ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc
    12421 gtgacaaacg atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg
    12481 aatttattct taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca
    12541 gccataacag aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct
    12601 gtaccaaaca cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca
    12661 gctaataatg caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc
    12721 aatgcatcat tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc
    12781 agtaagccaa acactggata aaagaggatc gttttaattc catccaatga ttttggtaag
    12841 ttcttgaata atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt
    12901 gcacctaaga atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc
    12961 aaaccagggc ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa
    13021 ccaaatgcag cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga
    13081 tttgataatt gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata
    13141 gcgattcctc caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg
    13201 taaattcttt gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca
    13261 tcaccatgga atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta
    13321 cggataccat cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct
    13381 acttttttgt ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat
    13441 cggtttttga ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc
    13501 gcttttttct tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct
    13561 gtaacggcaa caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct
    13621 tgttctgctt tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact
    13681 tcttctggtg tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga
    13741 gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg
    13801 aaaaacagat aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt
    13861 gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca
    13921 tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg
    13981 atatcggaaa tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt
    14041 ttatcagtcg cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc
    14101 atgattatcc tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat
    14161 caatcaattc gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc
    14221 cccatttaaa ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa
    14281 tcattgaatc ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat
    14341 aagttccttg ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct
    14401 gtgccccttc agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg
    14461 ttgtattgta aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa
    14521 caagtgcatt ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc
    14581 gaatgatatc aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg
    14641 acaatacgac gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt
    14701 cttcatctga aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt
    14761 tgatattgat tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt
    14821 caagccactt cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt
    14881 gagaatcgat ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga
    14941 agtcttcctt cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat
    15001 caattgatgg attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt
    15061 ttcacggaat tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc
    15121 caaaggtgca acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa
    15181 tgtctcttcg gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt
    15241 cgtgaatcct tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa
    15301 attttttagt tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt
    15361 tccacctaaa ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga
    15421 atttgtcact actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga
    15481 gccagcgtct aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat
    15541 ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa
    15601 taattgctct ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc
    15661 ttgtaaatca cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga
    15721 tttcacgatt ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag
    15781 cattttcacg atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa
    15841 tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa
    15901 aaagactggt tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg
    15961 cttcttgttt ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa
    16021 taaagtccca tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta
    16081 ggaacaaaaa agagacagac ggataataat gatgccggaa ccattattcg ataatagagt
    16141 tgtttatcca ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg
    16201 gtaatctaaa aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact
    16261 tcgccgcttt ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt
    16321 taacggtaat tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc
    16381 ttgatatttt tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc
    16441 ttcgattcca ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga
    16501 gcgattctcg tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct
    16561 ttctttataa tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt
    16621 tttaggaaca ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt
    16681 gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg
    16741 atcaaaccca agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca
    16801 gttaaagggg tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt
    16861 ttctacttgt tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact
    16921 ataatacatt tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg
    16981 aaattggttg caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc
    17041 gatttcatcc tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat
    17101 aaactcgcta taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat
    17161 gaagtctcga atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat
    17221 caataattga ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa
    17281 ccaactttcg tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata
    17341 cggatccgct tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa
    17401 aatagcccaa tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat
    17461 agtcatcaat aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat
    17521 ttaattataa ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac
    17581 gattacatga tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt
    17641 gaacgtacag ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca
    17701 gttgcaccaa aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa
    17761 aaagacaaga aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg
    17821 aaagagtttg actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg
    17881 ctgcccacgc ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat
    17941 tgcgatcaaa atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag
    18001 ggatcttatc tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt
    18061 gatacatctg gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg
    18121 cacgtagctg gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct
    18181 cagttttgga tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct
    18241 cgatcaaata aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat
    18301 tctttcgatc aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca
    18361 gagctatttc tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa
    18421 tttcagcttg atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg
    18481 gctgtcgcaa aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc
    18541 tctgaatctt tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata
    18601 atcacttttt cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct
    18661 tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg
    18721 gctctcaacg cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga
    18781 tattgtttga ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc
    18841 aatttctcta aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat
    18901 tttagtaaat cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg
    18961 ttttgtgtac caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg
    19021 ccatcaaatg caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct
    19081 tgatgtggat gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt
    19141 ttcagcaagg cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg
    19201 cttttacgcg caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc
    19261 acattgtaat cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca
    19321 taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt
    19381 gctaatgttt gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg
    19441 cttttttgca gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag
    19501 tgattgtata cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta
    19561 ttcaatactt caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct
    19621 tcataaaatt cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag
    19681 acccctggac cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag
    19741 cgtacggtct cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa
    19801 agtatgcaag aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa
    19861 aaagaaaaag tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa
    19921 caaaaaataa ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt
    19981 tctagaagta gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc
    20041 gctgtacctg tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct
    20101 tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg
    20161 atttctgcat tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg
    20221 atcacaagat ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct
    20281 tcttctgtag aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg
    20341 ttgttcaaga attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat
    20401 tcaacgattt ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc
    20461 gctgcttttt cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat
    20521 gttgatactt cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt
    20581 gcttttactt catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct
    20641 tgataatctt ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact
    20701 aattgatctt caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg
    20761 ccttttccgc catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta
    20821 tcgccatttt cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca
    20881 cgtttgatac gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact
    20941 agatctttat attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc
    21001 cagtcttgac ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg
    21061 ccagcttctt tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca
    21121 tataaagctt cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg
    21181 aatccaggta cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg
    21241 atttcgtctt gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat
    21301 gttgcagtca tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc
    21361 atgcatacct ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga
    21421 atccttgttt ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc
    21481 atttgctgca aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct
    21541 tcatacatcg tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct
    21601 tcgatgtatg tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt
    21661 aacacggaac taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc
    21721 tggtaacttg cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt
    21781 tcttcaatcg tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt
    21841 aattttgccc ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg
    21901 ataaactcat gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt
    21961 tccaacgaag gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt
    22021 tgtccggata attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca
    22081 taaaaatctg tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac
    22141 aagtcaaaat aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt
    22201 gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta
    22261 ttagcctcaa agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga
    22321 tattgctggt ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga
    22381 aaatcaatcg gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt
    22441 agcaaaacaa aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt
    22501 ttagatctga cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg
    22561 ttgacttcct ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa
    22621 aaatcgccta gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc
    22681 aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta
    22741 agttcttctt catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga
    22801 ccatgattga atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat
    22861 ccgtagatac tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca
    22921 agtgttaaac gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg
    22981 ataaaaaaac ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt
    23041 gtgtccgtat actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc
    23101 tctctttcaa aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc
    23161 tgctgttgct tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact
    23221 cacctccgta ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg
    23281 ataagatctg actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta
    23341 accatgtact agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa
    23401 gctgttgttc ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta
    23461 aacctacttc ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt
    23521 cttcttcaga tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt
    23581 cgatatatgc ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca
    23641 tatctttcaa cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc
    23701 ctgaataatt caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg
    23761 caaagatttt atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca
    23821 tgctgacata cattgggata tcatgttctt tacgaatcgt atcaaatttc ttagcgattt
    23881 ccgcactttc gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg
    23941 ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag
    24001 aagcaaataa acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga
    24061 ttttttgcgt actgtctccc tcttccaaca catttaaagc tagttcttca tccccatata
    24121 accaactgtt caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa
    24181 ctaacgaaac cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca
    24241 ttctttctcc tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag
    24301 tgtcgaaccg ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt
    24361 catcttctac cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt
    24421 ggatgctatc ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg
    24481 cttcgaattt ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat
    24541 ccatttttac tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg
    24601 atagataaaa acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata
    24661 taccaattta ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt
    24721 taaatgtttc cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca
    24781 ggtctgtgta aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag
    24841 ggactgcact ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt
    24901 cctgcagctg gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg
    24961 gaattgttgg aatgattgga ttaaaaaatg taacaccagg attgattgac ggcctagaaa
    25021 aattagaata ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt
    25081 atctggtcaa agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta
    25141 caggaaatat aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa
    25201 acgcccatcc acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg
    25261 aaaactttga agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg
    25321 atactgagat tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg
    25381 cgtttttaaa agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga
    25441 cagcacctga tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg
    25501 atggattcaa tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg
    25561 ttgagattga agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt
    25621 ttgcaggtga catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg
    25681 agaaaggtac ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc
    25741 gtcgtatcgc tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg
    25801 tcgatacatt gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg
    25861 cggggttagc aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt
    25921 tagccagtga atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct
    25981 tgagtcaaag tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg
    26041 gctacccttc acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct
    26101 ttacactgct attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg
    26161 cacaaatagc tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa
    26221 cttctcttgt attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg
    26281 tcatcgccca aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa
    26341 atgcctttta cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat
    26401 tgaaagaaat cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa
    26461 cgattgcttt gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg
    26521 gtgcgcatac tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta
    26581 ttgtctcaga gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt
    26641 atctaactac tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat
    26701 tacggggatt cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat
    26761 aaaagaacaa ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg
    26821 aaaatatctc ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat
    26881 agtaattaac tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac
    26941 cggaaggacg aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca
    27001 cttttttgat tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta
    27061 atttttgcgg atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt
    27121 tcatgatatt cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta
    27181 agaaaatcat gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt
    27241 ctacaacgta acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg
    27301 ctttgtggaa tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat
    27361 ttttttctgc caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc
    27421 caagttcatc caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg
    27481 ctcctttttc aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat
    27541 tgatatttaa cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa
    27601 acaaacgatt aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac
    27661 cggaaaggtc accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg
    27721 gaaattcttc tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag
    27781 catctaataa tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac
    27841 cgaaaaattt gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg
    27901 cgcttgcttt ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct
    27961 ggaacacttc gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac
    28021 ctgagattct tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct
    28081 ggcttaggac ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct
    28141 tgttggcttc accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac
    28201 tcctttaact taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc
    28261 tgatgtttct tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat
    28321 ggttgttgtc gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt
    28381 attttcttgt gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg
    28441 tgtaatctcc acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc
    28501 aattttacga gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc
    28561 accgatcgat gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact
    28621 gaaatcatag ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac
    28681 cgacttgctt ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat
    28741 acttaatact tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc
    28801 atctgtgacc atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt
    28861 gtcacctgta gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc
    28921 agatggtgtg aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt
    28981 ctcaaccgtt actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt
    29041 ttgaattttt agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac
    29101 gcggaacatc cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga
    29161 aagtttgaca gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga
    29221 aatcttgatt ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt
    29281 tggaacggac tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa
    29341 actgtaaaga atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc
    29401 ataccgtcta ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc
    29461 agttctctac gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta
    29521 tctaatgtaa tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg
    29581 acttcgctta taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata
    29641 ctttctgata gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact
    29701 gcaccgtcat gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata
    29761 tctgcatcta atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata
    29821 gtaattagcg caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag
    29881 atcatcttct catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca
    29941 agatgttcca gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg
    30001 tacgtaatga cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg
    30061 attcggataa caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc
    30121 agcagttgga tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc
    30181 aaaaaattca gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa
    30241 agttccccga tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag
    30301 tatatcatat gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca
    30361 taattttcag gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa
    30421 cttcagagaa ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt
    30481 gttccatttt tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg
    30541 tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt
    30601 tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt
    30661 cttggatagt cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg
    30721 aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt
    30781 ccaaagtcat tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc
    30841 aatagccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca
    30901 aaccatgtgt gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg
    30961 cttcttcaaa agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat
    31021 aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac
    31081 tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat
    31141 tggaatagac ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat
    31201 gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc
    31261 tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc
    31321 gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa
    31381 acatgtgtac caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata
    31441 cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat
    31501 aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt
    31561 cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg
    31621 cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt
    31681 cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca
    31741 agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc
    31801 aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg
    31861 cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac
    31921 ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt
    31981 ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac
    32041 attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc
    32101 gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa
    32161 gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta
    32221 tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg
    32281 ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta
    32341 cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca
    32401 cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga
    32461 tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa
    32521 tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt
    32581 cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat
    32641 agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg
    32701 ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt
    32761 tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga
    32821 tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga
    32881 atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat
    32941 catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat
    33001 caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac
    33061 caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag
    33121 gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat
    33181 gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa
    33241 aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa
    33301 ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt
    33361 cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta
    33421 atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt
    33481 tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt
    33541 tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct
    33601 cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag
    33661 caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag
    33721 caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat
    33781 ttgtcgtcac tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct
    33841 tgtacatatt cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg
    33901 caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta
    33961 ctacttttac tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta
    34021 attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat
    34081 aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc
    34141 atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt
    34201 tcttttattt ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga
    34261 caaacagtct aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg
    34321 tgttttaaca tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc
    34381 ctttatggtt catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg
    34441 tattgccgac aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg
    34501 caattgcagc gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac
    34561 gtgatgcacc ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa
    34621 atattcgata ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca
    34681 gtagataaac taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac
    34741 gaagctgagc aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt
    34801 gatgaatcta tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag
    34861 gagcgtagga caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg
    34921 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta
    34981 ttccgaataa atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa
    35041 gctgagcaaa ttatctccac caaataattt attcatattt ttgacttgcg catactaact
    35101 aattcttaaa ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg
    35161 gtgattcaca ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa
    35221 aaatagttgc ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg
    35281 tttattttaa acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg
    35341 gcaaacacaa atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact
    35401 cctaactcct ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca
    35461 cagcctagta tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact
    35521 ggctgatgaa atggattaat agatttcgat tattcaaagc gattttctat tggtggattg
    35581 tgttcccaac taacaagacg gattccaaag tcattatata actcttcaag agttaaccct
    35641 ttcctgttcc caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg
    35701 ccaagacctg gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa
    35761 atccagctcc tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata
    35821 attgttgtgc cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc
    35881 tacctgcatc tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc
    35941 tttccccatc tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc
    36001 ttggtggctt aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca
    36061 cagctataac aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc
    36121 gcaaatcacc tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc
    36181 tactgatcgt aataatattc gctat
//
LOCUS       DACDVT010000025        35427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDVT010000025 DACDVT010000000
VERSION     DACDVT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.025"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IWL52_001207"
     CDS             11..733
                     /locus_tag="IWL52_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9777782.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IWL52_001208"
     CDS             791..2830
                     /locus_tag="IWL52_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9777783.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IWL52_001209"
     CDS             complement(2939..4090)
                     /locus_tag="IWL52_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9777784.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IWL52_001210"
     CDS             complement(4244..4774)
                     /locus_tag="IWL52_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777785.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IWL52_001211"
     CDS             complement(4873..5223)
                     /locus_tag="IWL52_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9777786.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWL52_001212"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWL52_001212"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9777787.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWL52_001213"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWL52_001213"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9777788.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IWL52_001214"
     CDS             complement(7626..8288)
                     /locus_tag="IWL52_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9777789.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IWL52_001215"
     CDS             8472..10118
                     /locus_tag="IWL52_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9777790.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..11191
                     /gene="uxuA"
                     /locus_tag="IWL52_001216"
     CDS             10109..11191
                     /gene="uxuA"
                     /locus_tag="IWL52_001216"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9777791.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11299..12852)
                     /locus_tag="IWL52_001217"
     CDS             complement(11299..12852)
                     /locus_tag="IWL52_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9777792.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13000..14288
                     /locus_tag="IWL52_001218"
                     /pseudo
     CDS             13000..14288
                     /locus_tag="IWL52_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14414..15493)
                     /locus_tag="IWL52_001219"
     CDS             complement(14414..15493)
                     /locus_tag="IWL52_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9777793.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15596..16036)
                     /locus_tag="IWL52_001220"
     CDS             complement(15596..16036)
                     /locus_tag="IWL52_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9777794.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16093..16503)
                     /locus_tag="IWL52_001221"
     CDS             complement(16093..16503)
                     /locus_tag="IWL52_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9777795.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16683..17117)
                     /locus_tag="IWL52_001222"
     CDS             complement(16683..17117)
                     /locus_tag="IWL52_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9777796.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17217..18065)
                     /locus_tag="IWL52_001223"
     CDS             complement(17217..18065)
                     /locus_tag="IWL52_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9777797.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18454..20220
                     /locus_tag="IWL52_001224"
     CDS             18454..20220
                     /locus_tag="IWL52_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9777798.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20322..21581
                     /locus_tag="IWL52_001225"
     CDS             20322..21581
                     /locus_tag="IWL52_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9777799.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21728..23029
                     /locus_tag="IWL52_001226"
     CDS             21728..23029
                     /locus_tag="IWL52_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9777800.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23032..23883
                     /locus_tag="IWL52_001227"
     CDS             23032..23883
                     /locus_tag="IWL52_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9777801.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23896..24711
                     /locus_tag="IWL52_001228"
     CDS             23896..24711
                     /locus_tag="IWL52_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9777802.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24862..26028
                     /locus_tag="IWL52_001229"
     CDS             24862..26028
                     /locus_tag="IWL52_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9777803.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26258..26905
                     /locus_tag="IWL52_001230"
     CDS             26258..26905
                     /locus_tag="IWL52_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9777804.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26989..27933
                     /locus_tag="IWL52_001231"
     CDS             26989..27933
                     /locus_tag="IWL52_001231"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9777805.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28068..29519
                     /locus_tag="IWL52_001232"
     CDS             28068..29519
                     /locus_tag="IWL52_001232"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9777806.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29540..30376
                     /locus_tag="IWL52_001233"
     CDS             29540..30376
                     /locus_tag="IWL52_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9777807.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30376..31035
                     /locus_tag="IWL52_001234"
     CDS             30376..31035
                     /locus_tag="IWL52_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9777808.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31161..31850
                     /locus_tag="IWL52_001235"
     CDS             31161..31850
                     /locus_tag="IWL52_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777809.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31856..32812
                     /locus_tag="IWL52_001236"
     CDS             31856..32812
                     /locus_tag="IWL52_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9777810.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32856..33368
                     /locus_tag="IWL52_001237"
     CDS             32856..33368
                     /locus_tag="IWL52_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9777811.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33624..34172)
                     /locus_tag="IWL52_001238"
     CDS             complement(33624..34172)
                     /locus_tag="IWL52_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9777812.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34261..35424)
                     /locus_tag="IWL52_001239"
     CDS             complement(34261..35424)
                     /locus_tag="IWL52_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9777813.1"
                     /translation="MIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGFVLLRTT
                     FGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISAIIGILR
                     YIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFLSEKRLF
                     TMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEEKEDELI
                     IEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGITFQEILG
                     YIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTAIISVFL
                     VSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLLV"
BASE COUNT        11930 a         6452 c         6730 g        10315 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgcttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatataccag taggagaggt atggcccagg gagaggatca aagaactaaa
    10261 agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga
    10321 tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat
    10381 ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga
    10441 ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat
    10501 atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa
    10561 tgaatttgaa ttgccgggat gggaacctga gcgcttagct catatcaaat ccttgcttga
    10621 agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc
    10681 aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc
    10741 atactcgatt tttggattac ctcgaatcgt aaagaatcga gaagatttag attggttgtg
    10801 ccgcatagta gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga
    10861 tccagaaaat gatgtatatg ggattttagc agaattttgt caaagagatc gaatcccatt
    10921 tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg caccgcataa
    10981 atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt
    11041 taacgaatac atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg
    11101 atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat
    11161 cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa
    11221 taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat
    11281 ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag
    11341 tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc
    11401 ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt
    11461 caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat
    11521 cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc
    11581 acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg
    11641 tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg
    11701 ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa
    11761 cacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt
    11821 aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt
    11881 ttcaggtata aacaaatcaa atacagtaca aaaggactcc accatttctc ttgaataaag
    11941 aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga
    12001 ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca
    12061 aattggcaga ttaggtcgca aaatatctac agagggatca ataaaaaagt caatatattc
    12121 atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat tctctttaca
    12181 tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt caagtacttc
    12241 ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat cataaaattc
    12301 tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt gaatttgtac
    12361 ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca aaccgtacaa
    12421 tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac tatagtttgt
    12481 ttgctgactt aaactgataa tcgaatttgt ctgacagctg aacaaagaag tcaagatctg
    12541 ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt aagccggatc
    12601 aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca gttctgttag
    12661 attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat ttgattcaga
    12721 catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt cagataggaa
    12781 aagaaaaata tctaattttg tattgctttt tttcttcaag agaaacgttt taatcgcttc
    12841 ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga aaatattact
    12901 gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc tctactatct
    12961 tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc aaccatctta
    13021 atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt ccacacagag
    13081 gagatagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg aattaccttt
    13141 caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg acaaacagcg
    13201 accatcaaag caaaaattga ctattcagga gacttagcac ctatactaca atcagctgtt
    13261 acatttgaga atgttgatcc cgctttgtcg attggctatg acgagaacac tttgactatc
    13321 caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt gttaaaacca
    13381 gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcatca tttaacgccg
    13441 tatagtcagc ggcagacaat tgagcaagat tcagctatcg actcaggagg agatcttgtt
    13501 gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc tacagaaaat
    13561 ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac agaaactcaa
    13621 ccagagccag acaatgaatc aaaacctgaa ataacccctg gagaaaaacc agatgtagac
    13681 ccagaagaaa aaccagatgt gactccagaa ccagatacgg attctggaaa ccaaacagta
    13741 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagcc
    13801 ccagaagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta
    13861 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat
    13921 ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata
    13981 ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca
    14041 aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat
    14101 agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa
    14161 cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg
    14221 gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat
    14281 ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga
    14341 aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt
    14401 cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg atctttttca
    14461 ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc
    14521 caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt
    14581 tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg
    14641 taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag
    14701 gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg
    14761 ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat
    14821 ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat
    14881 cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg
    14941 agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag
    15001 caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat
    15061 tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag
    15121 gatctgcatc ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg
    15181 tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca
    15241 gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt
    15301 gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac
    15361 ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt
    15421 cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa
    15481 acacgttatt cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct
    15541 aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc
    15601 cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat
    15661 ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt
    15721 gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg
    15781 tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg
    15841 taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc
    15901 ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag
    15961 tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta
    16021 aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt
    16081 acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc
    16141 ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga
    16201 aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt cactttctga
    16261 ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat
    16321 caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt
    16381 ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg
    16441 attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt
    16501 cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg
    16561 gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc
    16621 tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa
    16681 cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc
    16741 tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag
    16801 tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt
    16861 gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta
    16921 ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag
    16981 gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca
    17041 tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt
    17101 tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt
    17161 ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat
    17221 tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca
    17281 aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc
    17341 acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg
    17401 gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca
    17461 ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt
    17521 gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga
    17581 ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa
    17641 tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat
    17701 aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct
    17761 gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa
    17821 agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata
    17881 ttcaacacta aaataactat agctcggtca aagcctaaag cagcttccag taatattcct
    17941 agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact
    18001 cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct
    18061 ttcatttatt cacttcctta attcattaat aatactctta tcttattata acttactttt
    18121 caccttaagt taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat
    18181 tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg
    18241 agaaattgat cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac
    18301 ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa
    18361 aaactgttac cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg
    18421 atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga
    18481 ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact
    18541 aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact
    18601 gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat
    18661 tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt
    18721 ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat
    18781 gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc
    18841 actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca
    18901 aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac
    18961 cgagaagact ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg
    19021 gaccttggca tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa
    19081 tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa
    19141 aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat
    19201 catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc
    19261 tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta
    19321 ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa
    19381 ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata
    19441 aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt
    19501 tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag
    19561 atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat
    19621 gcatttacag attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg
    19681 gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc
    19741 aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa
    19801 ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac
    19861 gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg
    19921 gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg
    19981 aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt
    20041 gaagataagc tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat
    20101 cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt
    20161 cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga
    20221 aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat
    20281 aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga
    20341 aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca
    20401 atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg
    20461 attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg
    20521 tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg
    20581 ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag
    20641 gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag
    20701 acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc
    20761 ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg
    20821 agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat
    20881 ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct
    20941 atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg
    21001 tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag
    21061 acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa
    21121 tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag
    21181 ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat
    21241 gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa
    21301 cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac
    21361 aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat
    21421 atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag
    21481 aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag
    21541 cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca
    21601 tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt
    21661 atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg
    21721 cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc
    21781 acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt
    21841 cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg
    21901 attggttact ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg
    21961 cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga
    22021 cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga
    22081 agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct
    22141 tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa
    22201 ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt
    22261 ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac
    22321 cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt
    22381 tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc
    22441 atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt
    22501 aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag
    22561 tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg
    22621 ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga
    22681 agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat
    22741 cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa
    22801 tttcaataat ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg
    22861 aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc
    22921 tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt
    22981 gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa
    23041 tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt
    23101 ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag
    23161 tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt
    23221 actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct
    23281 gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc
    23341 tatcgtttta tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg
    23401 actatggcag cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag
    23461 tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg
    23521 aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca
    23581 gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt
    23641 caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt
    23701 cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca
    23761 aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta
    23821 ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga
    23881 taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca
    23941 actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta
    24001 tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg
    24061 attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa
    24121 ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg
    24181 acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact
    24241 tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc
    24301 cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat
    24361 ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct
    24421 tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct
    24481 tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag
    24541 agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg
    24601 tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg
    24661 cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa
    24721 gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt
    24781 ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg taatagggta
    24841 gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt
    24901 tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat
    24961 acggacactc ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt
    25021 acaagtaacg cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag
    25081 ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga
    25141 aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa
    25201 gatatccatg cgcattatga cttaggtaac gacttttata aactttggct agatccaact
    25261 ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc
    25321 aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat
    25381 atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca
    25441 actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa
    25501 catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg
    25561 tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt
    25621 tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc
    25681 agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt
    25741 tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt
    25801 gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt
    25861 catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat
    25921 ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat
    25981 ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag
    26041 tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt
    26101 tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa
    26161 taaaagtttg gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt
    26221 aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac
    26281 tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc
    26341 tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa
    26401 agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga
    26461 tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc
    26521 aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt
    26581 cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga
    26641 gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct
    26701 ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg
    26761 aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt
    26821 caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt
    26881 actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt
    26941 tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa
    27001 acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc
    27061 atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag
    27121 agctgtacga tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta
    27181 gggagtatca attctatggg acttccaaac ttaggttttt cttattatct agagtatgct
    27241 cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg
    27301 atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg
    27361 atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat
    27421 tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga
    27481 atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat
    27541 cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat
    27601 acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag
    27661 tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa
    27721 atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta
    27781 tgtggcgcaa gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt
    27841 tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac
    27901 gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct
    27961 tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat
    28021 gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa
    28081 cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata
    28141 tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat
    28201 tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta
    28261 tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat
    28321 ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag
    28381 cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta
    28441 cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg
    28501 gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta
    28561 gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac
    28621 cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta
    28681 ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa
    28741 gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa
    28801 aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag
    28861 aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga
    28921 cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg
    28981 gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa
    29041 aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg
    29101 ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac
    29161 atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg
    29221 aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag
    29281 tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata
    29341 cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag
    29401 aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc
    29461 aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc
    29521 agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt
    29581 ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa
    29641 tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta
    29701 ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa
    29761 acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt
    29821 ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa
    29881 aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa
    29941 agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc
    30001 caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa
    30061 tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa
    30121 tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact
    30181 ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc
    30241 tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa
    30301 aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga
    30361 gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt
    30421 cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta
    30481 ggaaatctgg tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat
    30541 gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt
    30601 ctgttatatt ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt
    30661 acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct
    30721 tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat
    30781 atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat
    30841 ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca
    30901 aagatcatag gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt
    30961 tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg
    31021 catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt
    31081 gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa
    31141 tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat
    31201 accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc
    31261 aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat
    31321 gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg
    31381 gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc
    31441 ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct
    31501 ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag
    31561 ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg
    31621 agcgaggcgt aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag
    31681 aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta
    31741 gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt
    31801 tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta
    31861 ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg
    31921 aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct
    31981 taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc
    32041 ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa
    32101 tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc
    32161 acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa
    32221 acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg
    32281 gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag
    32341 ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg
    32401 atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc
    32461 tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa
    32521 acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc
    32581 aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg
    32641 aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa
    32701 aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga
    32761 ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa
    32821 tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag
    32881 aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag
    32941 gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg
    33001 aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag
    33061 tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc
    33121 cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa
    33181 aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta
    33241 tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt
    33301 atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca
    33361 tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca
    33421 atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat
    33481 ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt
    33541 tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat
    33601 cctacgagta ttattcgata catttatatc aataatcctt tttttatttt atctgcagct
    33661 tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc
    33721 aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct
    33781 ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg
    33841 actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa
    33901 caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga
    33961 gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca
    34021 gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa
    34081 gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca
    34141 gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa
    34201 gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata
    34261 ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa
    34321 aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc
    34381 accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat
    34441 tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa
    34501 ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg
    34561 gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat
    34621 gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag
    34681 catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg
    34741 gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac
    34801 atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt
    34861 gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt
    34921 agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt
    34981 ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga
    35041 ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa
    35101 tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat
    35161 caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc
    35221 aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac
    35281 cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat
    35341 cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc
    35401 taatgtgaca aataaaccaa taatacc
//
LOCUS       DACDVT010000026        32095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDVT010000026 DACDVT010000000
VERSION     DACDVT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.026"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..67
                     /locus_tag="IWL52_001240"
     CDS             <1..67
                     /locus_tag="IWL52_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9777814.1"
                     /translation="SLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(181..810)
                     /locus_tag="IWL52_001241"
     CDS             complement(181..810)
                     /locus_tag="IWL52_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9777815.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(944..1016)
                     /locus_tag="IWL52_001242"
     tRNA            complement(944..1016)
                     /locus_tag="IWL52_001242"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(981..983),aa:Thr,seq:ggt)
     gene            1230..1303
                     /locus_tag="IWL52_001243"
     tRNA            1230..1303
                     /locus_tag="IWL52_001243"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1264..1266,aa:Asn,seq:gtt)
     gene            1258..1407
                     /locus_tag="IWL52_001244"
     CDS             1258..1407
                     /locus_tag="IWL52_001244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777816.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            1900..3228
                     /locus_tag="IWL52_001245"
     CDS             1900..3228
                     /locus_tag="IWL52_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9777817.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3430..4263
                     /locus_tag="IWL52_001246"
     CDS             3430..4263
                     /locus_tag="IWL52_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9777818.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4279..5016
                     /locus_tag="IWL52_001247"
     CDS             4279..5016
                     /locus_tag="IWL52_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9777819.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5171..6139)
                     /locus_tag="IWL52_001248"
     CDS             complement(5171..6139)
                     /locus_tag="IWL52_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9777820.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6371..7204
                     /locus_tag="IWL52_001249"
     CDS             6371..7204
                     /locus_tag="IWL52_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9777821.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7238..8077
                     /locus_tag="IWL52_001250"
     CDS             7238..8077
                     /locus_tag="IWL52_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9777822.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8103..8663
                     /locus_tag="IWL52_001251"
     CDS             8103..8663
                     /locus_tag="IWL52_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9777823.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            8862..10583
                     /locus_tag="IWL52_001252"
     CDS             8862..10583
                     /locus_tag="IWL52_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9777824.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            10748..11890
                     /locus_tag="IWL52_001253"
     CDS             10748..11890
                     /locus_tag="IWL52_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9777825.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            11906..13117
                     /gene="thiI"
                     /locus_tag="IWL52_001254"
     CDS             11906..13117
                     /gene="thiI"
                     /locus_tag="IWL52_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9777826.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            13690..14337
                     /locus_tag="IWL52_001255"
     CDS             13690..14337
                     /locus_tag="IWL52_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9777827.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            14730..17375
                     /locus_tag="IWL52_001256"
     CDS             14730..17375
                     /locus_tag="IWL52_001256"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9777828.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            17685..18998
                     /locus_tag="IWL52_001257"
     CDS             17685..18998
                     /locus_tag="IWL52_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9777829.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            18988..19641
                     /locus_tag="IWL52_001258"
     CDS             18988..19641
                     /locus_tag="IWL52_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9777830.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            19696..20388
                     /locus_tag="IWL52_001259"
     CDS             19696..20388
                     /locus_tag="IWL52_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9777831.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20538..20738
                     /locus_tag="IWL52_001260"
     CDS             20538..20738
                     /locus_tag="IWL52_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9777832.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(20992..22671)
                     /locus_tag="IWL52_001261"
     CDS             complement(20992..22671)
                     /locus_tag="IWL52_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9777833.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(22673..22885)
                     /locus_tag="IWL52_001262"
     CDS             complement(22673..22885)
                     /locus_tag="IWL52_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9777834.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23278..25008
                     /gene="efrA"
                     /locus_tag="IWL52_001263"
     CDS             23278..25008
                     /gene="efrA"
                     /locus_tag="IWL52_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9777835.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25005..26765
                     /gene="efrB"
                     /locus_tag="IWL52_001264"
     CDS             25005..26765
                     /gene="efrB"
                     /locus_tag="IWL52_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9777836.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            26860..27057
                     /locus_tag="IWL52_001265"
     CDS             26860..27057
                     /locus_tag="IWL52_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777837.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27210..27695
                     /locus_tag="IWL52_001266"
     CDS             27210..27695
                     /locus_tag="IWL52_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9777838.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(27799..28392)
                     /gene="clpP"
                     /locus_tag="IWL52_001267"
     CDS             complement(27799..28392)
                     /gene="clpP"
                     /locus_tag="IWL52_001267"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9777839.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            28572..29099
                     /locus_tag="IWL52_001268"
     CDS             28572..29099
                     /locus_tag="IWL52_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9777840.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29190..29927
                     /locus_tag="IWL52_001269"
     CDS             29190..29927
                     /locus_tag="IWL52_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9777841.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            29931..30848
                     /gene="miaA"
                     /locus_tag="IWL52_001270"
     CDS             29931..30848
                     /gene="miaA"
                     /locus_tag="IWL52_001270"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9777842.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            30850..32079
                     /gene="hflX"
                     /locus_tag="IWL52_001271"
     CDS             30850..32079
                     /gene="hflX"
                     /locus_tag="IWL52_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9777843.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10619 a         5470 c         6791 g         9215 t
ORIGIN      
        1 ttctttagac agtcatgttc aattagtaga tctacgacca gaaaaattct ctggaataaa
       61 acaataactt tctctctatt gtccataaaa ctagtctcaa tcaagctgta ttgaaaaaac
      121 aagacaacat tcttaccatg gtaaataaaa agacgacaca ttttgtcgtc tttttttaaa
      181 ttaactattt tcgataaaag atcgcatagc ttgtgccatt atttctggct tttctctatt
      241 caattcatgt tttccgtttt taataaagag tagccggcca ttcgttgaaa gtttatataa
      301 acgttttgct gctggtttat tcgcatgatc tttggtaccg cataaaatca atttaggaag
      361 tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca agtgaacgaa gtagggtaag
      421 ggtttgtttt ttagatagac ctaatttttc aaatatctta gaaggtacgc agtaaaaata
      481 aatattctgg aaagtaagca ataagcgatt aggagactca aattgtggag cgactaaaac
      541 aacctttttt aaatatggag aaggagttgc aagatagttt aaagcaataa cagcgcctaa
      601 agataatcca cataaaataa aaggttcctt tatctgattc aattgtactt cgatgtattt
      661 ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa tgattgaaca atgaaagact
      721 tttgcaagaa taactattgg gtagataaga gattacatct ttccatgatt gttcgtcttg
      781 gcctagacca tgcaaaaaaa caattttcat attgctctcc tttttatagt tatcatatag
      841 actttctttg tactattaaa atataaacat tcatttctta cgtcataaaa taaaaaaaca
      901 gcgcagacac attgttaaac caacatgttg cactgtttaa ggaagccact tgccgggctt
      961 gaaccggcga cctcttcctt accatggaag cgctctacca gctgagctaa agcggcatta
     1021 tcattaatac aaataatagg ataacgaata ttaatgtatt tgtcaacaga attttttcga
     1081 aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa aataaaaata acaaaaaatg
     1141 cgaacggttt ctttgataat ataataaaaa aattcaaaaa aagtattgcg ttatttagaa
     1201 gaaattgata ttatattcat gtacttgatt ccggcatagc tcagttggta gtagcgcatg
     1261 actgttaatc atgatgtcgt aggttcgagt cctactgccg gagtaaataa agcttatgca
     1321 aagaaaaaga aatccaatca cttgggtttc tttttctttt attattatga tgaatcaagc
     1381 tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga gtagagttgg ctactcataa
     1441 tcaaccatac ttcccatcat cgctaaatac gaaataaatc agcattccta caccaatcaa
     1501 agcacaagct gcaccaagaa aagggatgac attcccaaag ttatctgtat tcataagtat
     1561 gccaccaccc acaaacaaaa agccgccagc aatcaggttt ttcattttat tcatctcctt
     1621 tcttacgtta tagtatttcc ttctttcaat gagtattttg aaagctatct tattgtatga
     1681 tgttcttcta ggcctcttct ttattataca ggccattttc gttatctcat agaaatattc
     1741 ctttttatct gaaaactatt gtataccgta tgacattttt cttcatgaag ttgtaagtat
     1801 attctcattc aaatgaaaat ttcatcatag aagagattgt atttctaaat tcttaagagt
     1861 aaaatacgaa gtataacttg gatttggaag gatgattgga tgaaagctat tacgcctgat
     1921 atgacaacaa aatttaacaa acagtttcaa gcaaatatca aacagcaggc attgcagcgg
     1981 gcagttgtga agaacggaat tactgcttct gctgaaaatg tttcagcaca tgtggagaat
     2041 acaccggtat tttctataga tttggctacg gggaaagtgg ctaatcaaat gcaatcgggg
     2101 cgttgctgga tgtttgctgc actgaacaca ttccgtcata agatgctgaa tcaattccat
     2161 ttaaaagagt ttgaattatc acaaaattac acttttttct gggataaata tgaaaaggca
     2221 aattatttct atgaaaacat cattgcaaca ggaaaagagg caatcgatag ccggaaagta
     2281 gctttcttac tcgctactcc tcaacaagat ggcggccaat gggatatgat cgtttcatta
     2341 ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg aaagtgcgaa tagttcaaat
     2401 tctcgagatt tgaataacta cttgaacaaa ttactgcgta aagatgcagt agtgttacgg
     2461 aaaatggttg ctcaaggaga aacattagac gctattgaag aaaagaaaga agaaatgcta
     2521 gaaagcattt ataacttctt agctatctca ttaggaacac ctccaaaaga attcgatttt
     2581 gagtaccgcg atgaagaaaa gaactatcat cttgaccgag gactgacacc acaaattttt
     2641 tatgataaat acatcggtgt aaaattagat gattatgtta gcgtgatcaa tgcaccaaca
     2701 aaagacaaac cattcaatcg ttcttataca gtagagatgc taggaaatgt cgttggcggg
     2761 aaagaagtca aatatctgaa tgttgatatg gaaactttca aaaaattagc gattgctcag
     2821 cttgaagaag gagaatctgt ttggtttggt tgtgatgtag gtcaatcttc tactagaact
     2881 agtggcatca tggccttaga tgcttattca atggatgatc tatttgatac ggattttaca
     2941 atgactaaag ctgaacgttt agattttgga gaaagtctga tgacccatgc gatggtattg
     3001 accggtgttg acttagtaga cgggcaatca acaaaatgga aagtcgaaaa tagctggggc
     3061 gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg cctggatgga cgaatatacg
     3121 taccaaatcg ttgtaagaaa agatttattg acgaaagagc aattaaatgc gttcaatgaa
     3181 gaaccaattg ttctatctcc gtgggatcca atgggtgctt tagcatagac gagtatttca
     3241 atgaagagta tgtagtaaag ataaaaagag ctggcttaag ccagctcttt ttatctttaa
     3301 agtccattcc ctgtttttta tggtccctgt gctataattc taaataaagg ataaggattg
     3361 gaaataatcc ttttatcatt aaaaatatga tgacgaagta gaataaaatt gggagtaggg
     3421 gggaaagtca tggatatcca aactgtttta tctgatctga aattaaatgg taaagtgatt
     3481 ccagttgttg gtggcgatgt taatcagacc tatcgtataa aaaccgaaca tcgtgcttat
     3541 tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg aagcagaagt agacggatta
     3601 aaagaactgt cagcattcgt acgtgtaccg gatacgtata tgctaggtga aacaagtgaa
     3661 ggcgcttatt tgttaatgga atggatcgag ccgggaaaag gggatcaacg agatcttgct
     3721 gcagctttag ctaatctcca tcagcaaaca gcgccacaat ttggttttcg taaagataac
     3781 tatttgggta cacttgttca gaaaaattct tttgaagaag attggtggac ttttttcttt
     3841 aaagatcggc ttgaatccca gatttctttg gcagaagaaa cgaatcgatg gaatgtacag
     3901 cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga aatcggttga accaaaaaag
     3961 atcactccac gcctcttgca cggagacctg tggagtggta atgtgttctt tgaccagcaa
     4021 gggcatccag tttttgtcga cccagctgtt tcttatggga atcgggaaca agatattgca
     4081 atgagccagt tattcggtgg tttccgaccg gaatttttag atgcttatca aactatattt
     4141 ccattagaaa agggatggaa agaccgactg ccgatttacc agttatatta tcttttggca
     4201 catttgaata tgtttggaga aagttacggt tcccaagtag atcaattact agaaaatttt
     4261 taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa agcatgaagt ttcatattat
     4321 gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt taggagtagt tatcaaaacg
     4381 tctgaagatc aaagcgacca gttgggaaga ggcagtgaat tcgtcaatag ctttggactt
     4441 acttgggtca tcacgaatta ttccatggag attaatcgtc tccctagagt cggtgaagta
     4501 atctcagtaa cgactcaagc catggagtac aataagtttt tctgttatcg gaacttctgg
     4561 atccatgatg aagatgggaa agaattagtc aaaatagaat ctgttttcgt attgatggat
     4621 tttgtgaatc gaaaaatgag cagtgttaat gaagagatca ttgctccttt tgagagtgag
     4681 aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga ttgattcggg cttcatgttg
     4741 ccatatcgtg ttcgttttta tgatatcgat agtaatcagc atgttaataa tgcgatgtat
     4801 tttaattgga tcattgacgt attaggatat gactttttga ctacgcatgt tccagaaaaa
     4861 gtaattattc gatttgataa agaagtagag tacgggaatg agatcgaaag ccattttgaa
     4921 caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa tccgaatgca agaacagctg
     4981 tattgtgaag caaatattat atggaaaaat aaataaaaaa gaggttgtga cagaaggggg
     5041 caacttcaag aaataagaag aaatttacga aaattgtttc tcaaattttt gtaaattttc
     5101 gacttagttt tcgaagaagc tatttttgtc acagcctctc tttttaagtt tagctattag
     5161 ataatgatgt ttagttttgg ataaacgaaa tcaattcttc acccgacaaa ccatagttta
     5221 cagcaatcaa tagttttaaa cgtgcttttt gactattaat actgttacag aaaataatcc
     5281 ccattttttc tagttctttt cccccgcctt cataatcata aacaggctct gcgattccat
     5341 tgaaacaacg agaaacaagt actacaggga ttttttggac aagaagcttt tccaaagcag
     5401 ctaaggcttg tggagggaga ttccccgcac ctaaagcttc gattaccaaa ccgtctactt
     5461 tcgtatgggc gatggcttcc aataagtccc cttgcatccc agcatatgct ttaacgatcg
     5521 ggatagtccc atctacagcg gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga
     5581 ataaaattcg attcttagtt actaagccga tgggacccaa tgtaggtgtt cggaaagtag
     5641 caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga atggatctca tcgttcatga
     5701 ccaccaaaac ccccttatct agcgcttctt cacaacttgc gacacgaatc gcactttcaa
     5761 aattataaag gccgtcactt ccaagttcat tacttgaacg cattgcccca gtcaaaacaa
     5821 ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg
     5881 taccgtgtgt gatcaccact ccggatatac ctgatgcata agcggacaag atacgttctt
     5941 ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg aagattgaag atatcttcaa
     6001 ctaataattc tacatgcttg gggattgtga tttctgcatc taataatggg tttgccacat
     6061 caggactaac accgcccgtg agcatgtttt ctttcatggc gatcgttccg ccggtgtgta
     6121 gtacaagtat ttttttcatt ttacagactt ccttacgaat aattttgcat agccgttaaa
     6181 tcttattgta tactatataa aagagttttg ctatcagtga agaataacga atagcctaac
     6241 ttatcataaa aaagctgacc ttattaaaat ggaaaagact caaccttgac ctcctaatac
     6301 aatggagtat ctggttgagt ttcttttaca agaaagcagc caaggaaatc aatcacgaag
     6361 gtggccggaa atgatcagaa tcggattaac atcgtttaaa gaacatgaca aattaactgg
     6421 taaaaagaat aacactcttt atgaatatgc agggtttcta cccttagtgg aattagatac
     6481 tgcctattat ggaattcccc gaaagtcctc agttgagaat tggataagtg aagtacctga
     6541 atcatttcgc tttgtagtca aagtctatca aggaatcagc ggtcaaggag actggaagcc
     6601 ttattatcag accgaagaag agatgattgc gcatttttta gatgttatgt cgccaatggt
     6661 agatgctgga aagatgtttt gctttttagt ccaattcccc tcatcattca aatgtacgaa
     6721 agaaaatgtc cagtacttgc gcaaacttag aaagtggttt gagggttatc ctgtttctat
     6781 agaattgcgg gattattctt ggtacagcga tgaatttctt gaacgtacaa gaaactttat
     6841 gtctagagcc gctttttctt tagccatcat cgatgagcca cagttgctga atacaacgat
     6901 tccttttgat ccatttgtca ccaatccgaa ctttgctctt tttcgattcc atggtaggaa
     6961 tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta
     7021 ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa
     7081 agaggtaggt gtgattttta ataataattc tggtggtgat gcggctgaaa atgcattagc
     7141 actgcaaaaa atattaaata tcaagccaga tggactcaat ccaacccaaa tggatctgtt
     7201 ttaagggctt attttattca agaaaggggc gaagtctatg atcaaagcta ttttttttga
     7261 tattgacgga acattggtca ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat
     7321 tgaatatgct cggaaaaacg gcattttatg cggtgtagca acggggcgca gcccagtaaa
     7381 gatcaaagaa atcattgatg aattggaatt agatatgtat gtcgtgtata atggtcaact
     7441 ggtttttact gcagatcgca cgattatcga ccaccctttt gaacaaaaag tattggaaca
     7501 tattgttgaa tttgcagatg agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt
     7561 agatggatca acgacgatgc tattaggaca atcgattttt atcaagcgac tagtcagctt
     7621 tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt ttagccctca
     7681 ccgacaaaag gatcgttatg aaacgttaga gatattaaaa gaaccaattt atcaatgtat
     7741 attgctcagt ccggagtcag aacagaaaaa gttagaacat cgctttcccg agtgtacgtt
     7801 tcagcgttca aatacgtata ctgtagatat catcccaaaa ggtggttcaa aattactagg
     7861 catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga
     7921 tcattacaat gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc
     7981 tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag aagatgggat
     8041 atacgaagga ctgaaacact tcggtctgat ttattagtca atgaaagaag gaaaaaatca
     8101 gtatggaagc agagacacct tttcaaaaag tagaaacatt ccacaaaact tttgatccaa
     8161 gaagaccaga gatacccacg gcttttacag aaaatgaagc tcatacacgt gctgcgttca
     8221 aaatagaaga acttgttgaa tttgttgcag ctacttcacc taataaagaa caactgcatg
     8281 aagctgttat gctgctgcac gaagcattag ataaagcaga agaaaaaata cttggtaaaa
     8341 cacagtgggg aagtgatccg ctggttgaac aagtagatgc actgacagat atcctttact
     8401 ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc
     8461 atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac cctgtaacaa
     8521 ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct aaaatcgcgg
     8581 cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct gatataggca
     8641 tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt tatacgatgg
     8701 aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc tataatctag
     8761 aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca gaacgccatc
     8821 tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat aatgttatca
     8881 tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt agttacttga
     8941 tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa gaattatttg
     9001 acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc
     9061 aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc tcgttcgctg
     9121 aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg ttcttaaaag
     9181 gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa caagtgacgg
     9241 aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca ctagctgttc
     9301 agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac gagcatgaat
     9361 acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc
     9421 agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt ttggataaag
     9481 cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg
     9541 agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac gagaaattga
     9601 tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca aaagtaagtc
     9661 gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg
     9721 aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa cgggaaatgg
     9781 aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc aaacatacaa
     9841 caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc tatacattga
     9901 accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa atggaacgtc
     9961 aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca gaagtgcgca
    10021 cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa gtagagatcg
    10081 acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat
    10141 tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc
    10201 cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa ctaagtgttg
    10261 tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct ctatgtgaag
    10321 aagatctaga attattagat aagaagacac atgatttagt agatgcagca gcattaacgg
    10381 agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg agagaagcaa
    10441 ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca ttagacgaaa
    10501 tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa gatttttatt
    10561 ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga gcaagacgga
    10621 ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt gaaaatttca
    10681 cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca aaaaagaggg
    10741 gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc aaagcttaga
    10801 tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc atgatttagg
    10861 caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt tgatccatgt
    10921 agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact gggtattaaa
    10981 aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag gagtcgaaca
    11041 tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag taagtatcgc
    11101 cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga
    11161 gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc agcccatcca
    11221 ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag atgctgttca
    11281 ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt ttgcaacatt
    11341 ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga aaaagggtcg
    11401 tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa gtgggacaga
    11461 aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag agataaagga
    11521 acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt tgaagacgta
    11581 tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt tatgttttgc
    11641 tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac aaatatttgt
    11701 ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat tgcacgcaat
    11761 gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg aaaacaattc
    11821 gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga aattttctgc
    11881 aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc gttatgggga
    11941 actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac aaaatgtcag
    12001 acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc ggatgcatct
    12061 tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa tatttgggat
    12121 ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt
    12181 tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta tcgctaaaag
    12241 aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag gtaatgctgt
    12301 ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc cattacgtgt
    12361 agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag ctggaggatt
    12421 gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg attctcctgt
    12481 agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt ttgctagtcc
    12541 gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca aactagcacc
    12601 ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag aagaaatcaa
    12661 aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt tgcgtttgac
    12721 cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat cattaggtca
    12781 agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga atacgccaat
    12841 cattcggcct gtagcgacaa tggataaact tgaaatcatt gaagtagcag aacagatcga
    12901 tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg ctcctccaca
    12961 gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt tagcaataga
    13021 tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag cagaatcgtc
    13081 taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa tagtgttttg
    13141 accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc gtttttgaac
    13201 taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa aagccggttc
    13261 ttttttacta actgaaaaat aaaattttat ttagagatca agctacagtt gataaatgat
    13321 ataaaaaaga gaatttccca tgaaatagcg aggttgtgac agaagtgggc agcttcaaga
    13381 aataagaagg aatttacgaa aattgctttt caaatttttg tgaaatttcg gcttattttt
    13441 cgaagaagtt acttctggaa caccgtgtat cagaggttgc gaaaaagctg ccctgcatca
    13501 agtaataaga acagccaaac aaaaatagtt cctcatattt tacgtttggt tgagcttatt
    13561 accgcagatg caagcttttg aacaccgttt tatttgtctc acaccctttt ccttcctact
    13621 tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat atgtgaaatt attaacaagg
    13681 agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag caactgcgaa aagattacct
    13741 ttatatttac gctacttgaa gatgctggat gactcaggta taagtagaat caaatcaaat
    13801 gagttcagcg aaatcaccca aattccttct gcaacgatcc gcagagactt ctcgcaatta
    13861 ggcgagctag gcagaagcgg atatggctat gatgtccctt ttcttatcga tgtattcaat
    13921 aatatcctta atactaaaga agaaaaacga atcgcattag taggatatgg aaatcttggc
    13981 aaagcattga aacacaacaa ttttagaaga aatgaaaatc taaatatcgt gtgcgtcttt
    14041 gataatgatc cagcactgat caatcgagta attgatggag aaatgatcta tccgattgat
    14101 cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag caatctctac ggtaccaagt
    14161 aaatattcac aatctgctat tgatgaaatt gtcaaaggaa atgtaaccgc tatacttaat
    14221 tttgcgcctg acagagtaac tgtcccagct tatgtcaatg ttcaatacat tgatttgaca
    14281 accgagttgc agacgcttat ttactttgat gagaattact cagaagtttt ttcttaggaa
    14341 aatttgagta ggcttattga tcttgcgaga actcgtcgta attctttcaa aaatttttta
    14401 gtactaaatg aagtggtttt ttcacgacca cttgttgact aaaaaaaaaa accattgtag
    14461 aatgaagcat aacgctgaac aagaggagta actggcacat tcattcttag agagagaact
    14521 atctgctgaa aggttctcaa tgacaaccag tgaaggtagc ttgggagttt ctgtcttgaa
    14581 tggagtagag atagacgagt cgtgatcgtt aactcacttg agaggcaata atgattgatt
    14641 attgcaattt aggtggtacc gcgtaaatca tacgtcctag aatacgaaag tattttagga
    14701 cgttttttag tttaaaggag gaaaacagca tgacggaaga aaataaccta tcaacaaaat
    14761 ataatccgca agaagttgaa gctggacgtt atcagcattg gttagaacaa gatttgttta
    14821 aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt aatcccgccc ccaaatgtta
    14881 ctggaaaact tcatttaggg catgcttggg atacgacctt gcaagatatg ctgattcgtc
    14941 aaaagagaat gcaaggatat gatactttat ggctgcctgg tatggaccat gcaggaatcg
    15001 cgacacaagc aaaagtagaa gcgaagctta gagagcaagg gatttctcgt tatgatctag
    15061 gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga agagtatgcg agccacatcc
    15121 gcgaacagtg ggctaaaatg ggcttatctt tagattacag tagggaacgt tttacattag
    15181 atgatggatt atctgaagca gtacgtaaag tttttgttac cttatacgaa aaaggtttga
    15241 tttatcgtgg tgaatatatc atcaactggg atcctcaagc acgaacagct ctatctgata
    15301 tcgaagttat tcataaagat atcgaaggcg ccttttatca tatgagctat gaactagcag
    15361 atggatcagg cgtagtagaa attgcgacaa cccgtccaga aacgatgttg ggagatacgg
    15421 cgattgctgt tcatccagaa gacgagcgtt atcaagcgct catcggcaaa aaagtgattc
    15481 tacctttagt caataaacaa atcccaatca ttgcagatga atatgtagat atggaattcg
    15541 ggacaggtgt agtaaaaatc actccagcac atgatccaaa cgactttgag gtaggtaatc
    15601 gtcatgatct tccacgcgta aatgttatgg atgatgatgg tacgatgaat gaactagcag
    15661 gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc aatcgtgcaa gatctgaaag
    15721 aaatcggtcg attgattaaa atcgatacga tgatccacag tgtgggtcat tccgagcgga
    15781 caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt tgtaaaaatg gcgccgttag
    15841 cgaaaaaagc cattgataac caagaaacag atcaagcagt ggaattcttc cctccaagat
    15901 tcaatcaaac gtttttacgt tggatggaaa atgtccatga ttgggtaatc tcacgacaat
    15961 tatggtgggg acatcaaatt cctgcatggt atcacaaaga aacaggtgaa atgtatgtag
    16021 ggatggaagc accagcggat agtgaaaact gggtacaaga tcctgatgtt ttagacactt
    16081 ggtttagttc agctttgtgg cctttctcaa caatgggctg gccagatacg gaaagcgaag
    16141 attataagcg atacttccct actagcactt tagtaaccgg ttatgacatc attttcttct
    16201 gggtaagcag aatgattttc caaagcttgg aatttactgg cgaacgtcca tttgagaatg
    16261 tcttgattca tggattgatt cgtgacgaac aagggcggaa gatgagtaaa tcattaggta
    16321 atggaatcga tccgatggaa gtaatcgaaa agtatggagc agatgctctc cgctggttct
    16381 tatcgaatgg ttctgcacca ggacaagatg tccgcttcag ctatgaaaaa atggatgctt
    16441 cttggaactt tatcaacaaa atctggaatg cttcacgttt cgttatcatg aatatcgaag
    16501 gaatgaccgt agaagagatc gatttatctg gagaaaaatc agtggctgac cggtggatcc
    16561 ttactcgttt gaatgaaacg atcgaacgag taactgagct atttgatcgt tttgaatttg
    16621 gtgaagcagg tcgacaactg tataacttta tctgggatga tttttgtgac tggtacatcg
    16681 aaatgagtaa agaagtactt tatggagatg atgctgtttc aaaacaaacc acccgtagca
    16741 tcttagtcca tacacttgat cagatcctac gtttattgca tccaatcatg ccattcgtta
    16801 cagaagaaat atgggaacat attcctcatc agggaaattc tctagtagtg gcagaatatc
    16861 ctgttgtacg tccagagttt gatgatgaaa cagcttctaa agggatggaa gtattgaaag
    16921 aattgattcg agccgttcga aatattcgtt cagaagtgaa tacaccattg tctaaaccaa
    16981 ttacgttgct aattaaaaca aacgatgcta aaatcgatca atttttagtt gaaaatacaa
    17041 gctatatcga acgcttctgt aatcccgaag agttgacgat atcaagtgag atcactgcgc
    17101 cagatctagc gatgtcagct gttctgacag gtgcagaaat cttcttgccg ttagcaggat
    17161 tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga actggaaaag tggacaagcg
    17221 aagtaaaacg tgtccaagga aaactttcta atgaacgttt tgtttctaac gcgccggaag
    17281 aagtagtaga ggcagagcgt gcaaaagaaa aagattattt agagaagcaa acggctgtca
    17341 aagaacgaat cgaaagtttg cgtactattc aataagaata gaaaaagaga gtgtgaaatc
    17401 agctagctat tactaaaagc tagctgattg aacatcgtta caggaaagag tttgagcctc
    17461 actcttagct tcgattcata aggagatgtg acataaattt tgtcgcatct cttttttagt
    17521 ctactcttat tatgtaggag aaggagggag gtggatcatg tatcaagagt caataaagta
    17581 gttgataaaa tcgttcctct ctctgtttct tttctctttg tcatactttt atttttctta
    17641 tgtgttctat ttgttataat aaaagagaaa tgggggaatt gttgatgacg attgaagaag
    17701 caatagaatg gatccatagt cgattaccat ttggttcacg tcctggcctt gaccgagtcg
    17761 aagctttatt agaagtggta gggcatccag agagaaaagt aaaaacgatc catatcgctg
    17821 gaacaaacgg aaaaggttct actgtagctt atttgagaag cctattagaa gaggctgggc
    17881 tgacagtagg tacatttact tcaccttata tcgaaacgtt caatgaacgg atcgcaatca
    17941 atggcaaaag cattccagac aatgatttga tccagcttgt aaaaaaatat cagccactga
    18001 tcaaacaact tgatcaattt gaagcagtag ctggtattac tgaattcgag acattaacag
    18061 caatggcatt tgattatttt ttgatgaaga aagtggacgt agcgattatc gaagttggct
    18121 taggcggctt attggattct accaatgtag ctgtaccaat gctgacggga ataacaacga
    18181 ttggacttga tcataccgaa atacttggag atacaattga agaaatcgct gcacaaaaag
    18241 ctggaatcat caaacaaggc gttccagtag taacaggaaa tattgcccca gaagctttta
    18301 ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct ttatgtatac ggggaaaatt
    18361 ataaaataaa ctatctgcat cctgatgaga tgtggggaga agtatttgaa ttttttaatg
    18421 aaataggaaa gatccctcaa ttaaccactc cgcttttagg gcaacatcaa gctgaaaatg
    18481 caggagtagc catcgaattg tattttgttt actgtcaatt ggaaaagctg cctttccgag
    18541 aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc aggaagaatg gagcgtttga
    18601 gtaacgagcc attagttgtt ttagatggtg cccataatac gcatgcaatg aaccggcttg
    18661 ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa cattttgttt tccgccttac
    18721 gaactaaaaa tgttagagaa atgctagaac gattgcagca agtaccaaat gtccatattt
    18781 atttgacgac ctttgaacat ccaaaagcca tcgaattaag tgaagtatca gatttagcaa
    18841 gtgagagagt atcaatcgtt tcattgtggc aattcggatt agcagatata ttggaaaaga
    18901 tgacaaatga agacgtatta gttgtgaccg gttcacttta tttcatttca gaagttcgcc
    18961 atttattaat acaattagga ggagatcatg aagtataatg cagtgatttt tgacatggat
    19021 ggtttgctgt tcgacacaga gttagtgtat tacgaagcca cacaagtagt agcagacaag
    19081 atgggttttc cttatgataa agacctgtat ttaaaattct taggtgtttc agatgaagaa
    19141 gtatgggcga attatcatat gattttctcg gaatttggca aaacaagtgt acaaagattc
    19201 atagatgatt cataccagga aacgcttgaa cgtttttcgc aaggagcggt acaaatgaag
    19261 cccggtgtgt tggaactcct ctctttttta gaagaacatc agattcccaa agttgtcgct
    19321 tccagtaacc aacgagcagt cattgaactt ttattgaaga agaacaatct gtttgaacga
    19381 tttgagacga ttgtttctgc agaaaatgtt caacgagcaa aaccagaccc agaaattttc
    19441 ttgttagcta atcagaagct aggtacagcg aagaaagaaa cattgatttt agaagattca
    19501 caaaatggta ttttagcagc ttctgcggct gagatcccgg tagtcatggt accagatctg
    19561 cttccaccat cccaagaatt gatcaaaaaa acagctgggg ttgtttcttc tttacatgaa
    19621 atccctgcat tgttaaaata aaaatccatc tcttttcgta tcttatttta gataagatag
    19681 aaaggagatg tttttttggg aaaacgattg atcaaagaag tacctactag ctctttacca
    19741 agggaacgta tggaaattta tggcgcggaa gctttatcgg atcaggagct tttggcaatc
    19801 ttactgcgta ccggtcaaca cccttatagt gtgatgtcga ttgctggaaa tctgttaaaa
    19861 acgtttggag gactggcctc tttacgacag gctactttgc atgagttaga agagattcga
    19921 gggataggac gcgtgaaagc aatcgaaatc aaagccttga tcgaattagg tcgacgaatt
    19981 cagacggatc atgaaagagc ggctccaccg gttcgtacca gctacgaatt ggcccagcaa
    20041 ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg tttgtattta tttggacacg
    20101 aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt cgctgaatca gtcagtcgct
    20161 catcctagag aaattttcca ttatgctgta cgttattgtg cagcaaggat cgttttagcg
    20221 cataatcatc cttctggtaa tgttattcca tcgcagcagg atatgaattt taccaaaagg
    20281 atacaaaagt gtggagaaat gatggggatc actgtactgg accacttgat catcggacgc
    20341 aaacgatatt ttagtttgag agaagaagga atgatggaag agaagtaagt tgctttcttg
    20401 caaaaaaaat gaagcaagtg tacaattata ttgtaatttt gtttgggaat agaagttgaa
    20461 acatctgttg cacctctatc gtacacgagc aatgttacaa tttaaattag tgcttttggc
    20521 acgaggagga tattattatg gaaaacggaa cagtaaaatg gttcaacgct gaaaaaggat
    20581 ttggatttat ctcacgtgaa gatggaagcg acgtattcgt tcacttctca gctatccaag
    20641 gtgacggatt caaaacatta gaagaaggtc aagcagtgac tttcgacgtt gaagaatcag
    20701 accgcggccc acaagctgca aacgttgtaa aagcttaatt ttatctgtaa atcaaattga
    20761 tggtagacat caatttttac gagagaacaa agcgagtaac aaaaatacct gccacaagag
    20821 acaatttctc ttgtggcagg tatttttttg tatgaaaaag taaataaatt taagaggcta
    20881 tgaaagagcc accttgtatc aaggaataag aacaaacaaa aatggcttct catgtttttc
    20941 atttgttttc tcttatattc aggacaaggt gctgatttca taacctctta tttattcttc
    21001 atctggtgtc attaccattg gcaaaatcat tggatgtctt tctgtacgtt cgaataagaa
    21061 aggctgaagc gcgccagtca tcgcttcttt caatttatat tctgaacagt caggtccatt
    21121 caaacattct cttaaagcgt ggaataagat tcgctgagct tcattgatca tttctcctga
    21181 ttctcgcatg tagacaaatc cacgagagag aatatctgga ccagccaaga tttctttttt
    21241 cttgatatcc actgtcgcga cagctaatac aagaccttct tctgaaagca ggtgacggtc
    21301 acgtaaaacg acattaccaa tatcaccgat cccgctacca tcgatataca catctccagc
    21361 cacaaaatgc ccagcaggac gcgcagaatc gctagttaac gctaaaacat cgccattttc
    21421 catgatgaag cagttttctg caggaacacc tacatcttgt gctagagaag catgtacttt
    21481 caacatgcgg aattctccgt ggacaggaat aaaatatttt ggcttcatca aacgaagcat
    21541 taatttttgt tcttcttgtc caccgtgacc ggaagtatgg atgttattga tttttccgtg
    21601 aatcacttct gcaccagctt cagacagcaa attgatcaaa tgattgacac ttgttgtatt
    21661 accaggaata ggtgaacttg aaaagatcac tgtatcgtct ggctggattt ggatttgacg
    21721 gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct tctccttgag aacctgtaca
    21781 taggatcatc gtttcattgg caggcaagcg attgatttcg tgtgcatcta caaatgtgcc
    21841 gtctggaact ttgatatatc ctaaacgttg accgttgacg atggcatttt ccatgcttcg
    21901 tccgaaaaca gcgattttac gaccagttgc cacagcggca tctgctgctt gctgcaaacg
    21961 gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct tcgatttttg aaaagatctt
    22021 caaaatagaa tgcccaatcg ttttttctga tttagtaaat gttggaatct cagcatttgt
    22081 actatcagat aaaaggcaca agactccttc ttccccgata cgagccattt tatgcaagtt
    22141 tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct ccagtcgcta cgatatttcc
    22201 agaaggtgtt ttcaccacga tccctaatgc atctgggata ctatgggtcg ttctgaaaaa
    22261 gctgacagaa gtctttctaa aacggatgat cgtatcttca ttgatctcat ggagctctgc
    22321 atctcgaagc aagccatgct catctaattt gtttgtgatc aacgctaatg cgagaggacc
    22381 agcatagata ggaatatttg cttgtttcag caagtagggt attccgccga tgtggtcttc
    22441 gtgcccgtga gtaatcacca aggcttttac tttgtgtaag ttttgtatga tgtagctgta
    22501 gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc gggaatttga tcccggcatc
    22561 aatcaaaatg atttcatctt ggaattgtac cccatacgta tttttcccga tttcacctaa
    22621 tccaccaata gcaaagacgc ctgtctcgtt atttttgatg gaaagtttca tattaaaact
    22681 ccactaatga gaaatcagca ttttcttttt cgtactctag atgacttcct tctagaagtt
    22741 gaacgaattc aatattgtat ggggtatttt cttccacacg ttggcgagcg tctacatcgc
    22801 tttttgcctc aatgtaaaga gattttgttt cttcccgttt agggttgcgt acttttgttt
    22861 cttgataata aactttatag atcattgttt tgtctccttt atctgcgtaa agcagtgatt
    22921 tagctagttg atttttttag acaacacaaa acgaggggac aagccacctg gtttttcata
    22981 tgctttgact agaatactgg attcgagtca aagagacaag cctcgttttt gcttatctgt
    23041 tcggatattt tttcaaccgt accttctgta taaaaaatat acaacaatta attgttattc
    23101 tagcataaaa cagcaaataa gtatagaaat caactagatt gaaagaaaaa atcaatgttt
    23161 atcatcccgc tataatcata atgacttttt tatgagagaa gtgttctatt aataggctcg
    23221 ttgtgctatc atttttagta atgaaaatgt aacaaaagat taaaaggaga tgagaaagtg
    23281 aaactaatgt ggcattatac catgcgttat aagaaattct tgtttttcaa tttcatatgc
    23341 gtatttggtt ttattttgat tgaattagga cttcctacga ttttagcacg tatgatcgat
    23401 gtaggaatcc gcaatgatga ttttgattat gtgaagcaac aagggatatt tatgatcgtt
    23461 attactgtaa tcggtattat tttgaatatc ttgttaggtt attttggttc gaggattacg
    23521 actaatatcg tagcagatat tcgagatgat ttgttcgaaa agatccaaag ttattcgcat
    23581 caggaatacg aaaatctagg cgtatcttca ttgatcactc gtacaacaaa cgatgcttat
    23641 cagatcatgt tgttcctgca aaatatttta cgtatcggtt ttatggcacc aatgatgttc
    23701 attgtcagtt tgtacatggt catgcgtaca agtgtgacac tcagtttgta tgtagtaggt
    23761 gcattacctt tactattatt ggcagtcgta gcgattgcta aggtgtctga accgttatct
    23821 aaaaagcaac aaaaaaactt agataaaatc aacagtatct tgcgggaaaa tctttcagga
    23881 ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag aaaaacgttt tgctgtagtc
    23941 aatgagaatt atgcttctag ttcaaaaagc ttattccgtt tgatggctgt tgcacaaccg
    24001 ggtttttatt tcttatttaa tatcgtaatg gtattgatca tctggagcgg ggcagtgcaa
    24061 atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt ttatcgagta tatattccat
    24121 gccttgttct cattcatgtt atttgctagt gtgttcatga tgtatccaag agcagctgtt
    24181 tcagctggac gtatccaaga agccctagat gccaaacctt cgattgtaga agatccaaat
    24241 ggtatatctg atacaaaaac taaagggtat atcgaatttc aaaatgtaac atttgcttat
    24301 ccaggccatt cgcaagagcc agtgatacga aatgtcagtt ttacagcctc tccaggagag
    24361 accgttgctt ttattggaag taccggtagc ggaaaatcta cgttgatcca attgatccct
    24421 cgcttttatg atgtgagtga aggaaagatc ttgatcgatg gagtagacgt gagagattat
    24481 cggctaagca agttgcgaga taagattggg tttattccgc aaaaagcgct gctatttact
    24541 ggaacgattg cagataattt gcgatacggg aaagaagatg ctacgcaaga agaaatggaa
    24601 cgtgcagctg agattgctca agcttcagaa ttcatttctc gaaaaccaga tggatttaat
    24661 gaacatttat cagaaggtgg ttccaatttt tcaggtggac aaaaacaacg attggcaata
    24721 gccagagcca tcatacgccg tccggaaatc tatatttttg atgacagctt ttctgcatta
    24781 gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa aagaaacgac agaatctact
    24841 gtattgatcg tagcacaacg tgtgggaacg atcatgcatg cagataagat catcgtatta
    24901 aatgaaggag aagtcgtcgg tatggggacg catcgggagt tgttggaaaa atgcccgatt
    24961 tattatgata ttgccgcttc tcaattatca aaggaggaat tagcatgaaa aaaacctttt
    25021 cttccttaaa acgtttagct cgatatatca gaccgtatcg tgagactttt cttttagtga
    25081 tcctatttac agttctgaca gtggctttca acgtggcttt gccatatgtt acaggacttc
    25141 ctactacaga aatcagccgt aatattgcca atggcgaatc aatcaatttt tcttatatat
    25201 tcaaatgtct catttggatc acagttgttg gaatagggta ttgtatttcg caattacttt
    25261 ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca tgatttgaga agagacatag
    25321 aagaaaaaat caatcgtctt ccggtttcat atttcgataa aaaccagcaa ggaaatattt
    25381 tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc tttacaacaa gcttttattg
    25441 gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc aatgatgttt tatatccagc
    25501 cgatgatggc gctgatctct atgatcatga ttccaacatc gatctggatc tcaaaacgag
    25561 tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa ttctcttggt gagctaaacg
    25621 gctacgtcca agaaaatatg acaggtttta gtgtattgaa agtatatgga cgcgaaaaag
    25681 aaacattgga aggattcaaa aaagttaatc acagcttgaa atactttggg tttcgtgcag
    25741 cattcatttc tggcttgatg atgcctctag ttcaattaac tgcctatgct acttatattg
    25801 gtatggctgt tctaggaagc ttttatgtta ttactggggc gatcgttgtc ggtcaactac
    25861 aagcattcat ccaatatatt tggcaaatca gtcagccaat ggggaacgtg acacaattgt
    25921 cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt attcgagatt ttagatgagc
    25981 cagaagaaaa agaaaacaaa gtagatgctt cactgccaga aacgatttta ggagatgtga
    26041 cattcgatca tgtcgatttt gcatatgatc cgaagaaacc attaatccaa gatttgaatt
    26101 ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc gaccggtgcc ggcaagacga
    26161 cattgatcaa tctattgatg cgtttttatg acgtagacaa aggagcaata aggattgatg
    26221 gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc gctatttggt atggtcttgc
    26281 aagatgcatg gctttatcaa ggaacgattg cagataatat ccgcttcgga aagcttgatg
    26341 cgacagatta tgaagtagtt gatgctgcga aaacagcaaa cgtagatcat tttatccgaa
    26401 cgatgccaga tggatatgat atggccatca attctgaggg agacaatgtg tctttaggac
    26461 aaaaacagtt gctgacgatt gcccgagcag tggtctccga tcctaaaatc ttgattttag
    26521 atgaagcaac aagttcagta gacacccgtt tagaagcgtt gattcaaaaa gcaatggatc
    26581 gcgtgatgga gggacggaca agtttcgtca tcgctcaccg tttatcgaca atccgtgatg
    26641 cagatctgat tctagtgatg gatcaaggtc agatcattga aaaaggaacg catgagagtt
    26701 tattggcaca aggtggattt tatgaaaaac tgtataacag ccaatttgct gaggaagcag
    26761 aataagtcat ataagaataa aacagtttaa aacttatttt ttgttctcta ttcttgataa
    26821 tggtaaagta aagttatcaa gaaaagtgag gacacggaga tggaacagtt tttaaatgaa
    26881 aaaattactg aagccaaaag agaagttgat cgtttaacgc acgtctgtca gacgaagcct
    26941 tttgattcag ctgatttttt tgaaaatgag tttcaaatcg aacgcttggc agcacaaata
    27001 gaagtgtacg aattaatttt aaaaaaagta atagaagaaa atatagatgt acaataaaaa
    27061 caggtaaaac gtcagttacc agatactgta aatagacatc tggcctgagc aggttttacc
    27121 tgtttttttt cttatgaaat aaatatatgc tatgatacct ttattcaata ggattagttt
    27181 ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt tatagcatca aatgccacag
    27241 tgattggtga tgtgacattg tcagaagatg tgacgatctg gtatcaagct gttttacgcg
    27301 gggacagtaa ttggataaaa ataggacaaa gaacgaatat ccaagatggt acaatcatcc
    27361 atgtcgatca tgatgcacct gtagatattg cggagaatgt gacggtagga catcagtgca
    27421 tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg gatggggacc acgatattga
    27481 atcatgcggt tattggtgag aatagtttga ttggtgctgg atcattagta acagaaggaa
    27541 aagttattcc tcctaatgtc ttggcttttg gtcgcccagc taaagtgatc cgtcctttga
    27601 caaaagaaga aatacaaaaa aacaaagaaa acatccagca ttatgtcgag ataggtcgga
    27661 tgcatgcgca aggaaaattc aaggagcaaa gataggcagt agcatccata aaataaaaaa
    27721 ggatggtcag tttgagttga ccatcctttt ttatttggtt tagataagag agcttaaaaa
    27781 agcttccttt gaaacagatt aatttaatga agaactgttt tccatgactt catcgatcaa
    27841 tccatatgct tttgcttgtt cagctgtcat atagttgtca cgatctgtat ctttttcaat
    27901 cacttcaagc ggttgtccag tgcgttcagc taaaattttg tttaagcgtt cgcgtgtttg
    27961 caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc ccttgagcgc cgccaagagg
    28021 ttgatggatc atgatctcag catttggtaa agcaaatcgt ttaccttttg taccagctgt
    28081 cagcaagaag cttcccattg aagcagccat acccataaca attgtttgga catctgcttt
    28141 gacaaagttc attgtgtcgt aaatagccat acctgctgta actgaaccac caggagaatt
    28201 gatataaaga tagatatctt tttcagaatc ttgagcgtct aagaaaagca attgggcgat
    28261 gatcgaatta gcaagatcat ctgttacttg gccgcttaac atgatgatac ggtctttcaa
    28321 taaacgtgag tagatatcgt aagctctttc acctcgtgaa gattgttcaa tgactgtagg
    28381 aattaaattc ataatagagt tcctccttga tttcgtttag aaatagtgta ccataattta
    28441 gtttctaata cctaaaatgt agtatacatt tttggtcaaa aaaggtcaaa taaaaaactt
    28501 aggcaaatta gtcgcataaa cccgctttat ggtatgataa gcagagaaac aaaacaagga
    28561 ggaacaacag attgatagtt ggtattactg gccttatcgg agtgctgaca ggtgctgtag
    28621 ttgtctatat cggcatgata ctgcaaatgc gtcacttgga aaataaagaa cagcgtagac
    28681 gtgaattgga attgaaagtc aaagaaatcg agacgctgaa tcaattaaat aaaaagatca
    28741 acgagatctt gcaaaaacgg acaacgctga tggaagaata tgtcagcttt gatgcatttg
    28801 atgattgtta tatcacgatt gacgattttg cgtatctcca atcattttca gcgcaaaata
    28861 gtttttatct gccaaattat attttagacg agctatttaa gaatatctcc catcgaaaag
    28921 tgattttatc tccagatgaa acaatgaaga ttggtggata tgcttataaa ggcggacgcg
    28981 tgattttaga gaattttacg gatagtctga ctgaaatgat caatgaaaaa aaagctcagt
    29041 tgaagactat gacgcgccag ccgattggtt tcttctcgaa agatgatgtt tggagctgat
    29101 attttgaagc attgccgttg cacatgagag cgtggtaatg ctttcttttt ttcttaatga
    29161 tacaatagaa ctgaacggag tgaagaaaga tgttacaaat ctatgcccat cgcggaagca
    29221 gcggcaccca cccagaaaat acgcttcctg catttgcaga agctgtacga gtaggtgcag
    29281 atggtatcga actagatgtc catctaagca aagatggtta tttgatcgtt atgcacgacg
    29341 aagaagtgga ccggacgact aacggtaaag ggctgattcg agaaaaaaca ttggaagaac
    29401 tcaaaaaatt gaatgcaggg ggcaaattca gcaaagaatt tccagcagct aaagttcctg
    29461 aattgagaga agtgttagga ctgctcctcc aaaaaaatta ccgagggatg ctaatgattg
    29521 aaattaaaac tgatcagtat gaatatcccg gtatcgaaga aaaattagcc gaaacattaa
    29581 aaagtagaga ttggcctttt caacatgcat attgcagctt tcatttggat tctttggaaa
    29641 aaatcgcatt actggaaccg gagatacagc tggacttatt gatgagtact tctgataaaa
    29701 agatccaact agcagcagaa accccttata tagaaggagt acatccaaaa aatacttggt
    29761 tgttcgaaca tccagtccag gcagctgatt ttccaaaagc aattcgtgca tggacgatta
    29821 acgatgaaga agaaatggcc aaatgtcaag aactcaaaat agatggaata ataactgatt
    29881 ttccggagaa agctcttcgg tggaaacaat caacgaaaag taggtaaaat atggaaaaag
    29941 tattagtgat cgtcggtccg acagcagtcg gtaaaacagc gctaagtgtg gaactcgcac
    30001 aactatttgg aggagagatc atcagtggtg attcattgca aatctataaa aatctggata
    30061 tcggtacagc aaaaatcaaa gaagaagaga tgaataatgt tccgcatcat ttgattgatg
    30121 tgatcgaacc tactcaacgt tactcagccg ctgattttca gcattcaggt cgacaattga
    30181 ttaccgagat tgcgaaccga gggcacctac ctatcatagc tggtggtacg ggtctctata
    30241 tccaatccct tctatatgat tatcagttag gagcgacaga agaagcgaat cctgagatta
    30301 gaaaaaagta tgaaacgtta gctgaaacaa ttggttccca agcattatgg aatcagttga
    30361 aagaaaaaga cccggcagct gctgagaaaa tccactggaa caatcaacgc aaagtgataa
    30421 gagctctgga agttttagag acgacaggac atagtatcac ggcacctaaa gaagagccaa
    30481 agcaattgta tgattacctc atgattggat tagatacgaa gcgtagtatt ctttatgaac
    30541 gaatcaatca aagagtcgat ttgatgctag cagaaggttt agtagcagaa gcgcgtaagg
    30601 tgtatgagct tggagaagtc caggccagtc aaggtattgg atataaagaa tttttccctt
    30661 attttgaagg gaaagaaaca ttggaagaag caacagaaca agtcaaacta cattcgagaa
    30721 gatacgcgaa acgtcagttg acgtggtttc gtaaccgttt ggatgcacag tggtttgatc
    30781 ttataagtga gccaaatcaa aaaagtcaga ttgagcaaac aatcaaaaaa tggctggagg
    30841 cggaataaaa tggaagaaaa agtgatcatc gtaggggtag aaaccgaagc aaaccagcgt
    30901 tacttttcag agtcaatgga agagctagtt cagctgacga ataccgcatc aggagaagtc
    30961 gtattcacaa ttacacaaaa gagaccacaa gtggatcggc aaacgattat cggtaaaggg
    31021 aaattacaag aattagttca acaagcggat gcgcatgaag cggatttgat catctttaat
    31081 catgagctaa ctccgagaca gagccaactg attaccgatg ctgttggtct tccggtaatt
    31141 gatcgcgtgc agcttatctt agatattttt gcgatgcgtg ctcgttcgaa agaagggaag
    31201 ctgcaggttg aacttgctca gttagaatac ctgctgcctc gtttagtagg gcaagggaaa
    31261 acattatctc gtcttggcgg tgggatcgga acaagagggc caggtgaaac aaagttagaa
    31321 acagaccgcc gacatatacg gaataaaatt tttgctgtta agaaagaatt gaaagaagta
    31381 gaagctcatc gagagcgcaa tcgccaaaaa agaaaaaata gcgagatttt tcagattgga
    31441 cttattggct acacgaatgc cgggaagtca actatcttga atctattgac acaagcggat
    31501 acttattcga aagatcaatt gtttgcaacg ttagatccat tgactaagag atggcgtttt
    31561 gctgaaggat ttgagattac tgtaacggat accgtcgggt tcatccaaga cctgcctacg
    31621 caactgatcg atgctttcca ttctacatta gaagaaagtc aaaatatgga tcttttgcta
    31681 catgtagtcg atgctagttc tccagatcgt attttacagg agcagactgt cttaaaattg
    31741 atggatgaac tgaatatgaa agaaatgccg atactgactg tttacaataa ggcagaccag
    31801 atcgacccag cgatgttcac gcctacgcta ttcccaaatg tattgatttc tactcagacg
    31861 aaagaaggta agatggcgtt gatcgaagca atcaaacgtc agctgatgga actaatgacc
    31921 ccttatacac tttctgtgcc aagcaacgaa ggacagaaat taagcgagtt gagaagacaa
    31981 acaatggttt tggacgaagc gtacatggaa gaaacaaacg aatatgaagt taaagggttt
    32041 gcaaaaacca attcaaaatg gttaagaaaa aaccaataaa attttatttt tttaa
//
LOCUS       DACDVT010000027        31944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDVT010000027 DACDVT010000000
VERSION     DACDVT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.027"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            51..1322
                     /gene="serS"
                     /locus_tag="IWL52_001272"
     CDS             51..1322
                     /gene="serS"
                     /locus_tag="IWL52_001272"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9777844.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(1871..3355)
                     /gene="guaB"
                     /locus_tag="IWL52_001273"
     CDS             complement(1871..3355)
                     /gene="guaB"
                     /locus_tag="IWL52_001273"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9777845.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(3860..4555)
                     /locus_tag="IWL52_001274"
     CDS             complement(3860..4555)
                     /locus_tag="IWL52_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9777846.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(4645..5745)
                     /gene="ychF"
                     /locus_tag="IWL52_001275"
     CDS             complement(4645..5745)
                     /gene="ychF"
                     /locus_tag="IWL52_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9777847.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(5754..5945)
                     /locus_tag="IWL52_001276"
     CDS             complement(5754..5945)
                     /locus_tag="IWL52_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9777848.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(5966..6856)
                     /locus_tag="IWL52_001277"
     CDS             complement(5966..6856)
                     /locus_tag="IWL52_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9777849.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(6843..7610)
                     /locus_tag="IWL52_001278"
     CDS             complement(6843..7610)
                     /locus_tag="IWL52_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9777850.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(7622..8335)
                     /gene="rsmG"
                     /locus_tag="IWL52_001279"
     CDS             complement(7622..8335)
                     /gene="rsmG"
                     /locus_tag="IWL52_001279"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9777851.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8567..10468)
                     /gene="mnmG"
                     /locus_tag="IWL52_001280"
     CDS             complement(8567..10468)
                     /gene="mnmG"
                     /locus_tag="IWL52_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9777852.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10485..11882)
                     /gene="mnmE"
                     /locus_tag="IWL52_001281"
     CDS             complement(10485..11882)
                     /gene="mnmE"
                     /locus_tag="IWL52_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9777853.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12226..12564
                     /locus_tag="IWL52_001282"
     CDS             12226..12564
                     /locus_tag="IWL52_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777854.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(12732..13490)
                     /locus_tag="IWL52_001283"
     CDS             complement(12732..13490)
                     /locus_tag="IWL52_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9777855.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13508..14326)
                     /locus_tag="IWL52_001284"
     CDS             complement(13508..14326)
                     /locus_tag="IWL52_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9777856.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14323..14679)
                     /gene="rnpA"
                     /locus_tag="IWL52_001285"
     CDS             complement(14323..14679)
                     /gene="rnpA"
                     /locus_tag="IWL52_001285"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9777857.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(14797..14931)
                     /gene="rpmH"
                     /locus_tag="IWL52_001286"
     CDS             complement(14797..14931)
                     /gene="rpmH"
                     /locus_tag="IWL52_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9777858.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15454..16788
                     /gene="dnaA"
                     /locus_tag="IWL52_001287"
     CDS             15454..16788
                     /gene="dnaA"
                     /locus_tag="IWL52_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9777859.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            16986..18116
                     /gene="dnaN"
                     /locus_tag="IWL52_001288"
     CDS             16986..18116
                     /gene="dnaN"
                     /locus_tag="IWL52_001288"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9777860.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18345..18587
                     /gene="yaaA"
                     /locus_tag="IWL52_001289"
     CDS             18345..18587
                     /gene="yaaA"
                     /locus_tag="IWL52_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9777861.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            18574..19698
                     /gene="recF"
                     /locus_tag="IWL52_001290"
     CDS             18574..19698
                     /gene="recF"
                     /locus_tag="IWL52_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9777862.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            19695..21641
                     /gene="gyrB"
                     /locus_tag="IWL52_001291"
     CDS             19695..21641
                     /gene="gyrB"
                     /locus_tag="IWL52_001291"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9777863.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            21661..24132
                     /gene="gyrA"
                     /locus_tag="IWL52_001292"
     CDS             21661..24132
                     /gene="gyrA"
                     /locus_tag="IWL52_001292"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9777864.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24368..24667
                     /locus_tag="IWL52_001293"
     CDS             24368..24667
                     /locus_tag="IWL52_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9777865.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            24717..25229
                     /gene="ssb"
                     /locus_tag="IWL52_001294"
     CDS             24717..25229
                     /gene="ssb"
                     /locus_tag="IWL52_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9777866.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25255..25491
                     /gene="rpsR"
                     /locus_tag="IWL52_001295"
     CDS             25255..25491
                     /gene="rpsR"
                     /locus_tag="IWL52_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9777867.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            25634..27607
                     /locus_tag="IWL52_001296"
     CDS             25634..27607
                     /locus_tag="IWL52_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9777868.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGESAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            27613..28065
                     /gene="rplI"
                     /locus_tag="IWL52_001297"
     CDS             27613..28065
                     /gene="rplI"
                     /locus_tag="IWL52_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9777869.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            28339..29706
                     /gene="dnaB"
                     /locus_tag="IWL52_001298"
     CDS             28339..29706
                     /gene="dnaB"
                     /locus_tag="IWL52_001298"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9777870.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            30486..31778
                     /locus_tag="IWL52_001299"
     CDS             30486..31778
                     /locus_tag="IWL52_001299"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9777871.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9700 a         6262 c         6329 g         9653 t
ORIGIN      
        1 aataatggga tggatcagtc tttttttatt ttcaaaaagg aggaaatcat atgttagatg
       61 taaaaatgat gcgacaaaat attgaagaag tacaagaaaa attgatgact cggggcgtaa
      121 aaaaagaaac attagctgaa ttcatggaac tagatgaaaa tcgtcgtaag ctactagtca
      181 aaagtgaaga attaaaaaaa tatcgaaatg atgtttctgc tgagatcgcc caattgaagc
      241 gtgcaaaaga agatgcttct gctaaaatcg ctgaaatgaa agaagtcggt ggcaacatca
      301 aagcattgga tactgaaatt gctgaagtgg atgaaaaatt gaaacaaatt gcaactactt
      361 tgccaaacct tccccatgat tctgtgccag ttggaacaga tgaagaggac aatgtagaaa
      421 tccgtcgttg gagcgaacca cgtacatttg cttttgaacc aaaaccacac tgggaaattg
      481 cagaaaatct tgatatttta gattttgaac gtggcgcaaa agtagccggt agccgttttg
      541 tctattacaa aggattaggt gctcgattag aacgtgctgt ctacaacttc atgcttgatc
      601 agcatattta cgaacacggc tatactgaaa tgatcactcc ttatatcgtc aacagtaaag
      661 cgatgtttgg tacgggacaa tttccaaaat tcaaagaaga tgttttccaa ttggagaata
      721 ctgatctaac attaatccca acagctgaag taccattaac taactattat aatgatgaaa
      781 ttttagatgg tgcagattta cctatttatt tcactgcact aagccctgct tttcgttctg
      841 aagccggaag tgctggtcgg gatacacgag gactgattcg tcttcaccaa ttcaataaag
      901 tagaaatggt caaattttcg gatgtagagc attcttacga agaattggaa aaaatgacag
      961 ctgatgcaga agatatcttg aaaaaattgc atttgcctta tcgtgtgatg gccttatcaa
     1021 ctggagacat gggcttttct gcagctaaaa cttatgactt agaagtatgg atacctgcac
     1081 aagatactta tcgtgaaatc agttcttgtt ctaactgtga agatttccaa gcacgccgcg
     1141 caatgatccg ttatcgcgat aatgatggaa aaatccacta tactcatact ctgaatggtt
     1201 caggacttgc agttggccgt acagtagctg ccattttgga aaactaccaa aatgaagatg
     1261 gttctgtcac agttccagaa gcgctcgttc cttatatggg gaatctgaaa gttattaaat
     1321 aaagaggctg tgaaaaagcc gcttagtttc gagtattaag aaaaattttg tgaaaatggc
     1381 tttccacatt tttgcataaa ttggacttaa taccgagaaa ctggcttttg aacaccattt
     1441 atcgagaggt tgtgaaatca gcgaattaac ctgagtatta gagaaaaaaa tggaaaaata
     1501 gtccctcata ttttaccgtt tgttgaacta ataccgaggg tcagctgatt gaacaccgtt
     1561 tagtggagag gtcgtaagcc taccccccag ctcctagtta caaggacaaa ttcaaaaaac
     1621 agctcttcat attttatgaa tgttgtactt gtgattcgga ggtggcaagg gaacactgtt
     1681 tattcaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag aaattcacga
     1741 aaattgcttt tcaaattttt gtgaatttct tcttatttcc gaaggagttg cttctacttc
     1801 cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc
     1861 tttaatgttt ttaagattca actgaatagt ttggtgcttc ttttgtgatt tgtacatcat
     1921 gagggtgtga ttcttttaat ccactgccgc tcatttggat aaattgcgct tcatcacgta
     1981 attgctgtag attggctgcc ccaacataac ccattcctga acgcaaaccg ccgatcattt
     2041 ggaaaataat atctgcaaca cttcctttat aagcaacccg accttcgatt ccttctggta
     2101 ctaatttatt tgcttcattg acactacctt ggaagtaacg gtcttttgaa cctttttcca
     2161 tcgcgcctaa tgagcccata cctcggtatg ttttgaatcg acgtccttgg aagatttcga
     2221 attcacctgg tgattcatct gtccctgcca acatacttcc tagcatcact gcatgtccgc
     2281 cggcagctaa tgctttgacg atgtcaccag aatatttgat ccctccgtct gcaataatcg
     2341 ctttgccata ttgacgagca actgatgctg catcataaat agctgtgagc tgaggtacac
     2401 ctacaccggc taccacacgt gtcgtacaaa tcgatcctgg tccgatacca acttttacga
     2461 catctacacc tgcatcatat aaagcttttg tcgcttcagc cgtcgcaacg tttcctgcaa
     2521 tcaatgttgc atctgcaaaa gttgaacgga tttcttgaat ttttcggatc acacctgcac
     2581 tatgtccatg agctgtatcg atgatgattg catctgcccc tgcttctaat aaagcattcg
     2641 cacgctcaaa tgtatcgctt gttacaccaa cggctgctgc tacaagcaat cgaccatgtt
     2701 catcttttgc tgcatttgga aattcgatca ctttctcgat gtctttgatc gtgatcaatc
     2761 cgcttaaacg accttcgtta tctacgattg gtaatttttc gattttatgt ttttgcaaaa
     2821 tcttttctgc atcttttaga gaggtaccta ctggggcagt cactaagttg tcttttgtca
     2881 tcacatcagc aatcggcatg tgataatctg ttacaaaacg catgtcgcgg tttgtaatta
     2941 tccctaccaa ttttctgttt tcaagtgttt caacgattgg cacaccactg atacgatatt
     3001 tgctcatcaa ttcttctgcg tctgctacaa gatgctgtgg cgttaagaag aacggatcaa
     3061 tgattacgcc actttcagaa cgtttgactt ttcttacttc atccgcttgc tggctgatcg
     3121 tcatgttttt atggatcaca cctagtccgc cttgtcttgc catagcaatg gccatcttgc
     3181 tgtcggtaac agtatccata cttgcactga tgattggaat attcaactta atatttttag
     3241 ctaattgtac gctcatatcc acttcgtttg gtaacacgtg actttctgct ggtatcaata
     3301 agacatcatc aaaagtaaat ccttttttcg tgaactttgt ttcccagttg gacattttta
     3361 gtaccactcc tctaatttag tttatctatt aaaataattg attttttcat ttaagtcaat
     3421 gatctacttg gaatcaagtt agctttctca tagaatacca atataaagcg tttacatctg
     3481 tgtatattaa aagagattgt aaaaaagctg tcctgcatca agtaataaga acagccaatc
     3541 aaaaacagct tctcatgttt ttgaattggt tgggtttatt accgcagatg caaacttttg
     3601 aacaccgttt agtggagagg ctgtgaaaaa gctgcttagt ttcgagtagt aagaaaaatt
     3661 ttgtgaaaat ggctttccac atttttgcat aaattggact taataccgag aaactggctt
     3721 ttgaacaccg tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta
     3781 ttatttaact atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat
     3841 agctgatctt tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt
     3901 ttttcttcaa gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg
     3961 gatctaatac tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc
     4021 aaagaagtgt cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc
     4081 gatctgctcc gggatattca tattgataga tgtatttgta catcatataa aagacccagc
     4141 caccgattgc agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg
     4201 aaaacaatcc ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat
     4261 ctaaccacat caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg
     4321 cttccgttct ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt
     4381 ctgcaactaa agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat
     4441 tcgctgcttc taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt
     4501 ttgtttctaa actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct
     4561 aatagtcctc cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat
     4621 tctacagcta gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc
     4681 ttgtacgaca taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat
     4741 gtttccgtaa tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc
     4801 gaaatctgaa tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca
     4861 agcacgtact tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc
     4921 agcacggatc aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc
     4981 tttgtcttct tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac
     5041 ttctgcattt tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc
     5101 atctgctacg tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa
     5161 aaatagtgat ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt
     5221 tccttcttcc agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt
     5281 atctttggtt ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc
     5341 tgctaacact agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc
     5401 tacgtgagta atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg
     5461 gcggatatgg cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat
     5521 tccagcaata tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc
     5581 tgttaagcgt tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt
     5641 cgcaaaggga tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga
     5701 ttttcctacg ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct
     5761 ttctctgcat ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc
     5821 atgacgatat gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact
     5881 tcccagcgat tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg
     5941 tacataaaaa tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa
     6001 gaattcgagt caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt
     6061 tttcttggat agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga
     6121 tataatatgg tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt
     6181 gatttaactc agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac
     6241 gattggctaa tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc
     6301 ccatagataa acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc
     6361 gcaaataatt ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg
     6421 ttagtttcag gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat
     6481 cttctcgctg caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc
     6541 taacgatagc agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc
     6601 ctgcaataat ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac
     6661 ctgattgttc aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg
     6721 gctgatacgg atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta
     6781 catccaccgt ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc
     6841 ctttactttt cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct
     6901 cgagaacgtg gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga
     6961 atatttcttg gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct
     7021 acgacttcat ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc
     7081 tctaattctg gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat
     7141 ccttccaatg cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat
     7201 gaattgattg tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga
     7261 ctgcttactt ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat
     7321 tcgatctctg ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca
     7381 gtgatcaaag ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt
     7441 gtcacatcag gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc
     7501 actagcaata cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt
     7561 gttttgccga cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct
     7621 cctatttgat tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg
     7681 gtttcttttt ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg
     7741 cctcttcttt gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt
     7801 cctcttcact ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca
     7861 aacacaattc cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg
     7921 atgcacgaaa gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat
     7981 cgattcctaa taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa
     8041 cgatcgttat ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc
     8101 ccgaaccaac atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa
     8161 gtgaatcgta aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca
     8221 tcttttcgtt ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat
     8281 cggtaagctc tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata
     8341 ttcctttcat tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat
     8401 ctctagacat gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga
     8461 tagacttttt ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga
     8521 gcaatttctg caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac
     8581 tgactttcgc aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt
     8641 ttacgccact gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt
     8701 gttttgcttc tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt
     8761 tcgcttccat gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat
     8821 atttgatttg gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa
     8881 taaaccgtag aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta
     8941 caccatcttt taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg
     9001 gtttgattcg tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca
     9061 aataattctt gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc
     9121 gcaaatctgc attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac
     9181 gataaggttc gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt
     9241 cacttcgttt gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg
     9301 caatcagccc ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg
     9361 tataaagatt ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta
     9421 cgtcatattc gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag
     9481 aatgaagcat gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga
     9541 cttcttccgt atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa
     9601 aacgcacgat cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc
     9661 ctgtaaacat tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg
     9721 tataggtcag ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc
     9781 taaaatgatt cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac
     9841 ttgattttac tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt
     9901 ttagatgatt cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca
     9961 attcaccgat aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg
    10021 cccggtagct cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca
    10081 gttcttcgac gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca
    10141 tttctgttga ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt
    10201 ttcctgtgtt caacatacgc atttggatat aggttttatc gatattctta cccatttctc
    10261 cgcctaacgc atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat
    10321 tacatggcat gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca
    10381 tacgtgcagc agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt
    10441 catacgcatc tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa
    10501 aattgactga ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct
    10561 agatagtccc agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca
    10621 atacctgcaa tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg
    10681 gtattagaaa cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa
    10741 atagctgatt ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt
    10801 tcattttcga tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta
    10861 tttagaagaa tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct
    10921 gtcagtggtt cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt
    10981 cggcttcttt cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta
    11041 tcgattagct tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc
    11101 gttcctgcaa tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt
    11161 gatttgccga catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc
    11221 ccttgcttgg atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca
    11281 agcaataact tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact
    11341 tctacttgtg ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga
    11401 ttgccgtcta actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa
    11461 tccatcacag cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg
    11521 aattcgcctg gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg
    11581 actacaacga tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt
    11641 ggtgctctca tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg
    11701 atatgtccat aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga
    11761 taaactttat ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca
    11821 ccttctccag gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc
    11881 attttttcac ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc
    11941 acaataaacg gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga
    12001 ttatcacact tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt
    12061 ctgcttccat taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat
    12121 tcgaagagaa gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa
    12181 atggtagcgc tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga
    12241 tctgctgcat tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg
    12301 aatatcaacc agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct
    12361 gatttgtttg acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta
    12421 aagacgaagg tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag
    12481 caaaaaaaac gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa
    12541 tttatccaaa caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga
    12601 cagtttcgat ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa
    12661 gaatgatttc tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa
    12721 aagtaatgga atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc
    12781 tcaccttccg aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta
    12841 cgttcaaatg caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca
    12901 gccgtgcgat cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca
    12961 ttgactacta cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat
    13021 tgcaatgcat tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct
    13081 aaatggaaaa caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca
    13141 agctgttttg aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct
    13201 agctcactta cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca
    13261 gctgttggtt cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt
    13321 tcgctgatac ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct
    13381 ttttttcctt cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc
    13441 agacctagtt ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc
    13501 tcccctatta tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt
    13561 cgcggattct ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca
    13621 gcagcgtttg tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg
    13681 ctaaattgac acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg
    13741 atgcattcga ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg
    13801 ctgctaaaat cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat
    13861 gcccttgttt caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca
    13921 tttgtactaa taaaggaaga caaccagcat atggattgac gccattttct gcatataaac
    13981 gctgctgctc ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct
    14041 gcagtgcttt taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat
    14101 gcatcaacgg caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg
    14161 cattaccaaa tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc
    14221 aaattcctgt actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca
    14281 aactaacgat accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc
    14341 tctgtcgatt atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata
    14401 agtcaacccc ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc
    14461 ttcttttata gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc
    14521 gttgccgacc ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc
    14581 caaacggtaa acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg
    14641 aaactctcgt tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat
    14701 ctctcactta cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa
    14761 aaagagtata aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc
    14821 tttgcgacga cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa
    14881 tccgtgaact ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac
    14941 ctccaaaatg ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa
    15001 gtagtgattt tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat
    15061 ttttttctat gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata
    15121 aaaggctggt tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt
    15181 tcctgtgtat tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag
    15241 aggaaatcta ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt
    15301 tttgacaact gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa
    15361 aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag
    15421 ttattcactt tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat
    15481 gaattaaaag caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa
    15541 acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt
    15601 cataaagaat attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta
    15661 tcaggaaatg agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt
    15721 atagaagaaa aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac
    15781 cctaaatata cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca
    15841 gcgctcgttg tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc
    15901 gtgggacttg gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag
    15961 cctaatgcca aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg
    16021 atccaaaaca aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta
    16081 gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact
    16141 ttcaatgcct tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac
    16201 gagattccga aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat
    16261 attacaccgc cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa
    16321 cgtctagaga tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt
    16381 cgtgaattag aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat
    16441 atttcgacta gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct
    16501 caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa
    16561 gatctcaaag gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat
    16621 ttggcacgtg agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag
    16681 gatcatacaa cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc
    16741 agtatgcaaa atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct
    16801 atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt
    16861 tccacagctg ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg
    16921 ccctattact tttattattc tttttaacta ataataaata aacaaacatt caaaggagat
    16981 acatcatgaa agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac
    17041 gagctatttc aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac
    17101 aagaaggttt gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg
    17161 ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac
    17221 gtttctttag cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag
    17281 aaaataaaca agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg
    17341 cagataacta tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac
    17401 acgtattgac taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc
    17461 caatcttgac aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg
    17521 attctcaccg tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata
    17581 ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa
    17641 tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct
    17701 attcccgttt gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta
    17761 atacagaggt tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac
    17821 tttctcatga aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt
    17881 tatacggaaa ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca
    17941 gtggcgatcc attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat
    18001 ttggagatat gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac
    18061 caacggaaga tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat
    18121 gattttcaat gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca
    18181 gaaggcccta aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta
    18241 gattaaatcg tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag
    18301 gtataataga gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca
    18361 ttctaaatac ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta
    18421 gtggcggaca agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag
    18481 aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa
    18541 ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa
    18601 aattatcgaa attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta
    18661 ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg
    18721 cgaagtcatc ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg
    18781 attcaaggag agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa
    18841 aaaggccgaa aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga
    18901 caaatgaatg tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag
    18961 accaggcgga agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat
    19021 cttgtccaat accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa
    19081 aaaaaacaaa ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc
    19141 agcaaagtat tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct
    19201 ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc
    19261 gattctttag aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac
    19321 caaaataaaa agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat
    19381 ctttcctttt ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga
    19441 acaactgcac taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa
    19501 tatccaatcc ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat
    19561 ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat
    19621 gtaaaaaata aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa
    19681 ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg
    19741 ccagtcagat tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata
    19801 tcggttcgac tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga
    19861 tcgatgaagt attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca
    19921 gtatcaccgt tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac
    19981 gtcctgcagt tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg
    20041 ggtacaaagt ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta
    20101 cacagcttga tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg
    20161 gagctgtagt ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc
    20221 actttacgcc agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg
    20281 ctacccggat ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca
    20341 aacgagaaga agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg
    20401 tcgaatactt gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg
    20461 aacagcaaga tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata
    20521 tcatgagttt tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca
    20581 agactgcttt aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa
    20641 atgacgacaa cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca
    20701 agcatcctga ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac
    20761 ggacagtcac tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa
    20821 gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga
    20881 aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa
    20941 aattagcgga ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag
    21001 attcggccgg cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta
    21061 ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag
    21121 agatccggtc tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga
    21181 aagctcgcta ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc
    21241 gtacattgct gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg
    21301 tctatattgc tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac
    21361 aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac
    21421 caaaaccaag cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg
    21481 aaacaactat ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg
    21541 cagcagatca gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca
    21601 tcgaggaaaa tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga
    21661 atgagtgaag aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac
    21721 tccttcatcg attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat
    21781 ggtctgaaac ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg
    21841 gataaagccc ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct
    21901 catggagact atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt
    21961 tatatgctag tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct
    22021 atgcgttata ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat
    22081 aagaacactg ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta
    22141 cctgctcgct tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc
    22201 acgaatatcc cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat
    22261 aatcctgatg tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca
    22321 ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct
    22381 attaccgtac gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc
    22441 gtgacagaat tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg
    22501 cacagagaca agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt
    22561 atgcggatcg tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta
    22621 tataaattga catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt
    22681 gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa
    22741 gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta
    22801 gaaggtttgc gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct
    22861 aaatcagatg acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag
    22921 tctcaagcta ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc
    22981 gaaaatgaat accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga
    23041 ccagaacgtg ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt
    23101 gataaacgac gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta
    23161 atcgaagaag aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca
    23221 aataatgaat tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac
    23281 aatgacgact tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt
    23341 acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca
    23401 gccaaaggaa taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca
    23461 atcattgcgg tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa
    23521 ggtacagtaa aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc
    23581 gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct
    23641 aatatcatta ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc
    23701 gatatgggac gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc
    23761 gttggggcag ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac
    23821 ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa
    23881 acggcgaaca tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat
    23941 gaagacatca tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt
    24001 tctcaaactg gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa
    24061 gttgtcacga tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga
    24121 tcagaagaat agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat
    24181 ctggttattc ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt
    24241 tcattctgta ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa
    24301 ttaattagat aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg
    24361 aatatcaatg gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga
    24421 agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga
    24481 agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg
    24541 tgaaggtatc tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga
    24601 atttgaccgt cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga
    24661 agcttaattg ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga
    24721 ttaataatgt tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa
    24781 gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg
    24841 gcaatcgcga ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag
    24901 caaactatgc tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt
    24961 atgataatca gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat
    25021 tgctggaaag tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg
    25081 gtcaatcagc tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca
    25141 acggtatgcc tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg
    25201 acatttcaga cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca
    25261 caacaaagaa gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt
    25321 gaatatatcg attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa
    25381 atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca
    25441 atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta
    25501 ggaattgaat gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa
    25561 cctctattcg tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata
    25621 tgggaggaaa attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat
    25681 cctgtttata ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc
    25741 ttcggtcatt ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg
    25801 gcttatttta aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct
    25861 agcttatgtc tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa
    25921 aatcgaatgg ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga
    25981 agaattgcta gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc
    26041 agataaaaca tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat
    26101 tacccaaaac cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt
    26161 atctattgat aactatgcag atgccattga taaattggat gacaaagaga tttcttattt
    26221 gaactctttc gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa
    26281 aagaatcaac gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat
    26341 ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc
    26401 tatcaccctt agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga
    26461 agctcagaat aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga
    26521 ggtaaaagaa ggagcaaaac ctgtttatta tgggggggaa tctgctaaga atgtcaaacg
    26581 cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg
    26641 cgatgtgttt atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg
    26701 tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga
    26761 gatcattcca gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat
    26821 gcaactaatc tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat
    26881 ggtggattac cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa
    26941 gattgtgatc attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac
    27001 ttatatcgaa tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca
    27061 aagcagcaaa acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt
    27121 tgtagataca aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta
    27181 tttaaggtct tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc
    27241 gacatacttg gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt
    27301 tgtggcggca gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga
    27361 tacattgctt tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg
    27421 cttgattggg atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc
    27481 cttaggtggc ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga
    27541 ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa
    27601 ggagtaatcc aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg
    27661 gacatcaaag aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca
    27721 aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa
    27781 cacgaagctg aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat
    27841 actgtagtcg aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct
    27901 tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa
    27961 atcgaattag aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat
    28021 catgaggtga cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg
    28081 atgttcagct ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa
    28141 acagctgcag caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt
    28201 tttgggaaca gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta
    28261 ttacactttc ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga
    28321 agacaaggag cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt
    28381 gaagctgaac aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc
    28441 atggagtaca tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca
    28501 atgatcgagt taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg
    28561 gaacaagcca atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta
    28621 gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg
    28681 ctgcgtacat tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa
    28741 gaagttcaaa caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga
    28801 aatcgaagcg gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat
    28861 cgactagctc aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat
    28921 aaaatgacag caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta
    28981 ggtaaaacag cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct
    29041 gttgcgatat ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca
    29101 gaaggttcga ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg
    29161 aatctgatcg ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca
    29221 gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat
    29281 ctaggcttga tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt
    29341 caacaagaag tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta
    29401 cctgtcattg ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca
    29461 gtgctaagtg atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc
    29521 ttgtatcgag atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca
    29581 aaaaatgaca acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca
    29641 gttgaactac tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa
    29701 ttttaaagaa gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag
    29761 gagtaaccgc aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac
    29821 aaaagtcttg tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt
    29881 attagctcag caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc
    29941 aggtcaaaac gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct
    30001 cggtcacaag tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg
    30061 actcagagtt aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat
    30121 ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct
    30181 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc caaaagtagc
    30241 ttcttcgaaa aataagtcga aatttcacaa aaatttgaga agcaattttc gtacattcct
    30301 tcttatttct tgaagctgac cacttctgtt acaacctctt ttttaataac cattctaaaa
    30361 agataaagtt cgttttttaa gcgtttttat aaaataaaaa taaaaatcat tcgattttat
    30421 ttgaaatatg tatctattat tgatacaatg gttcaggatt aaataatata gaacgaggtg
    30481 ttcacatgtc atctgtagta gtagtaggta cgcaatgggg cgatgaaggg aaaggaaaaa
    30541 tcacagactt tctcagtgaa aatgctgaag tgattgcacg gtaccaaggt ggcgacaatg
    30601 cagggcacac aattaagttt aacggtgtga cttataaact gcatttgatt ccttctggga
    30661 ttttttataa agacaagatc agtgtgatcg gaaacggcgt tgtagtcaat cctaaatcat
    30721 tggttaaaga attagcttat ttacatgaaa aaggtgtaga aacaagtaat ttgcgtattt
    30781 ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt ggaccaatta caagaagatg
    30841 ctaagggcga aaataagatt ggtacaacga ttaaaggaat cggacctgcc tatatggata
    30901 aagcagctcg tgtaggtatc cgtattgcag acttattaga taaagaaatt tttgaagaac
    30961 gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt aaaaatgttt gattcagaac
    31021 cacttgattt tgacgatatt tttgaagaat attatgaata cggacaacaa atcaaacaat
    31081 acgtcacaga tacttccgtt atcttgaatg atgctttgga tgcaggcaaa cgcgtgttgt
    31141 ttgaaggcgc acaaggtgta atgctggata tcgaccaggg aacttatcct tttgttactt
    31201 cttctaaccc agtagccggt ggggtaacaa tcggaagcgg tgtagggcct tcaaaaatca
    31261 ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt cggtgatgga ccatttccaa
    31321 ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt aggacgagaa tacggtacga
    31381 caactggtcg tccgcgtcgt gtaggctggt ttgattctgt agttatgcgc cattctaaac
    31441 gagtttcagg tattaccaac ctatctttaa actcgatcga tgtattaagt ggattggaaa
    31501 cagtaaaaat ttgtacggct tatgaattag atggcgaact aatctaccac tatccagcaa
    31561 gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga attgccaggc tggtcagaag
    31621 acattacagg atgcaaaaca cttgcagaac tgccagaaaa cgcacgtaac tatgtacgtc
    31681 gtatctcaga gcttgttggt gtacgtatct caacattttc tgtcggacct gatagaacac
    31741 aaacaaacat cttagaaagc gtctgggcgc aaatctaagg ataaaaacga cgactgaatc
    31801 aggacgttct taccagagcg agtcgaagtg tttatctata cgataataat aacttgaaga
    31861 ggtagtgaaa gagacgttta gccttattgc cgataggctg tctcttgaac actgtttatt
    31921 cagaataagg gactgtgaca aaaa
//
LOCUS       DACDVT010000028        29103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDVT010000028 DACDVT010000000
VERSION     DACDVT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.028"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IWL52_001300"
     CDS             complement(<1..1030)
                     /locus_tag="IWL52_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9777872.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IWL52_001301"
     CDS             complement(1112..1801)
                     /locus_tag="IWL52_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9777873.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWL52_001302"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWL52_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9777874.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IWL52_001303"
     CDS             complement(2328..2831)
                     /locus_tag="IWL52_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9777875.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWL52_001304"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWL52_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9777876.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWL52_001305"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWL52_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9777877.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWL52_001306"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWL52_001306"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9777878.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWL52_001307"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWL52_001307"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9777879.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IWL52_001308"
     CDS             complement(6443..7462)
                     /locus_tag="IWL52_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9777880.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IWL52_001309"
     CDS             complement(7635..8522)
                     /locus_tag="IWL52_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9777881.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWL52_001310"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWL52_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9777882.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWL52_001311"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWL52_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9777883.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWL52_001312"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWL52_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9777884.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IWL52_001313"
     CDS             9753..10397
                     /locus_tag="IWL52_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9777885.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IWL52_001314"
     CDS             complement(10453..10956)
                     /locus_tag="IWL52_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9777886.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IWL52_001315"
     CDS             11553..12518
                     /locus_tag="IWL52_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9777887.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IWL52_001316"
     CDS             complement(12617..13789)
                     /locus_tag="IWL52_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777888.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IWL52_001317"
     CDS             complement(13776..14921)
                     /locus_tag="IWL52_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777889.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IWL52_001318"
     CDS             complement(14935..15867)
                     /locus_tag="IWL52_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777890.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IWL52_001319"
     CDS             15984..17063
                     /locus_tag="IWL52_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9777891.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IWL52_001320"
     CDS             17060..17689
                     /locus_tag="IWL52_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9777892.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IWL52_001321"
     CDS             complement(17799..18026)
                     /locus_tag="IWL52_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9777893.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IWL52_001322"
     CDS             complement(18129..20717)
                     /locus_tag="IWL52_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9777894.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IWL52_001323"
     CDS             complement(20737..21396)
                     /locus_tag="IWL52_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9777895.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IWL52_001324"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IWL52_001324"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9777896.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IWL52_001325"
     CDS             complement(22169..23035)
                     /locus_tag="IWL52_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9777897.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IWL52_001326"
     CDS             23144..23785
                     /locus_tag="IWL52_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9777898.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IWL52_001327"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IWL52_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9777899.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IWL52_001328"
     CDS             complement(25226..26125)
                     /locus_tag="IWL52_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9777900.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IWL52_001329"
     CDS             complement(26127..26924)
                     /locus_tag="IWL52_001329"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9777901.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IWL52_001330"
     CDS             complement(26902..27606)
                     /locus_tag="IWL52_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9777902.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IWL52_001331"
     CDS             27737..28312
                     /locus_tag="IWL52_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9777903.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="IWL52_001332"
     CDS             28430..>29103
                     /locus_tag="IWL52_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9777904.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8561 a         6167 c         5233 g         9142 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtccct taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta gtggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DACDVT010000029        27902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDVT010000029 DACDVT010000000
VERSION     DACDVT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.029"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..1103
                     /locus_tag="IWL52_001333"
     CDS             18..1103
                     /locus_tag="IWL52_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9777905.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAKIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKYKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            1914..2246
                     /locus_tag="IWL52_001334"
     CDS             1914..2246
                     /locus_tag="IWL52_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9777906.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKAVVQCKKYI
                     IQKDKVSEEMIYYTGKRKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            2837..3166
                     /locus_tag="IWL52_001335"
     CDS             2837..3166
                     /locus_tag="IWL52_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777907.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            3153..3494
                     /locus_tag="IWL52_001336"
     CDS             3153..3494
                     /locus_tag="IWL52_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9777908.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3608..3847
                     /locus_tag="IWL52_001337"
     CDS             3608..3847
                     /locus_tag="IWL52_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777909.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            3914..4153
                     /locus_tag="IWL52_001338"
     CDS             3914..4153
                     /locus_tag="IWL52_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777910.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            4239..4910
                     /locus_tag="IWL52_001339"
     CDS             4239..4910
                     /locus_tag="IWL52_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9777911.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4963..6939
                     /locus_tag="IWL52_001340"
     CDS             4963..6939
                     /locus_tag="IWL52_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9777912.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            6952..7707
                     /locus_tag="IWL52_001341"
     CDS             6952..7707
                     /locus_tag="IWL52_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9777913.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            7704..8528
                     /locus_tag="IWL52_001342"
     CDS             7704..8528
                     /locus_tag="IWL52_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777914.1"
                     /translation="MNKRKWLFVCVAALFSTAAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            8818..10929
                     /locus_tag="IWL52_001343"
     CDS             8818..10929
                     /locus_tag="IWL52_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9777915.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDNKNFDMVSDGEKYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(10967..13024)
                     /locus_tag="IWL52_001344"
     CDS             complement(10967..13024)
                     /locus_tag="IWL52_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9777916.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(13138..13350)
                     /locus_tag="IWL52_001345"
     CDS             complement(13138..13350)
                     /locus_tag="IWL52_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777917.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            13537..14004
                     /locus_tag="IWL52_001346"
     CDS             13537..14004
                     /locus_tag="IWL52_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777918.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            14001..14243
                     /locus_tag="IWL52_001347"
     CDS             14001..14243
                     /locus_tag="IWL52_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777919.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14261..14563
                     /locus_tag="IWL52_001348"
     CDS             14261..14563
                     /locus_tag="IWL52_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777920.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            14635..16689
                     /locus_tag="IWL52_001349"
     CDS             14635..16689
                     /locus_tag="IWL52_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777921.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            16689..19301
                     /locus_tag="IWL52_001350"
     CDS             16689..19301
                     /locus_tag="IWL52_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9777922.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            19298..21829
                     /locus_tag="IWL52_001351"
     CDS             19298..21829
                     /locus_tag="IWL52_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777923.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKIFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            21879..22211
                     /locus_tag="IWL52_001352"
     CDS             21879..22211
                     /locus_tag="IWL52_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777924.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22212..22805
                     /locus_tag="IWL52_001353"
     CDS             22212..22805
                     /locus_tag="IWL52_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777925.1"
                     /translation="MRVKGQNLVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22968..24992
                     /locus_tag="IWL52_001354"
     CDS             22968..24992
                     /locus_tag="IWL52_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9777926.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            24992..26197
                     /locus_tag="IWL52_001355"
     CDS             24992..26197
                     /locus_tag="IWL52_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9777927.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            26213..26857
                     /locus_tag="IWL52_001356"
     CDS             26213..26857
                     /locus_tag="IWL52_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777928.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26868..27674
                     /locus_tag="IWL52_001357"
     CDS             26868..27674
                     /locus_tag="IWL52_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777929.1"
                     /translation="MSKKKGERQRFKKRKSYLQCLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYKQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTKNKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27696..>27902
                     /locus_tag="IWL52_001358"
     CDS             27696..>27902
                     /locus_tag="IWL52_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777930.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYIAAKK"
BASE COUNT        10081 a         4304 c         5629 g         7888 t
ORIGIN      
        1 gaagaaggag aaatttaatg tctacagaaa atcaatctca aaaaatgatt attaaaaatt
       61 ggggatataa agcaacgaat gtttatattg aaaaatatta taaatttcct agttttttaa
      121 ttcagaattc taaatactct aaaatgaaag acagctcaaa aatagcctat atgcttttta
      181 aaaatgaatt tcgtagagcc ttacatgaaa aatggatcga cgatgatggt ctattatatt
      241 tggaatttac acaagcagac ctaatggaca aattaaattg ctaccaaggc aaagtaaaaa
      301 ccattttaaa cgagttagaa acatataatt tgatttcagt cgtaaaagga gaatttaacc
      361 caaaaacgaa aaaaaatgaa aaaaataaat actatttact tcaacctgaa ataagtgccg
      421 atgatctttt tattcaaaat tctttcgatg acgatttaag cgaaactatt gataaatcaa
      481 cctcttctcg gaatgcggaa atcgcacgtc gagaggaaac tgctaaaacc cttgataata
      541 ctggaaatgc ggaaatcgca cgtcgagaaa aaacagctga aacccttgat aacactggaa
      601 atgcgaaaat cgcacaagat tattatatta ataaatcttt agacactaag agacacttaa
      661 aagacactga aacagaccaa ttacaaaatc aagttttgct agataacttc gttgaaatca
      721 tgaaagaaga cagcataaac acatttgtac cagaaaaggt attaagcttg ataaaaacat
      781 tttctaacac ttacgaagaa gcccaacaaa ccgtcaccac aatacacaac gcaaaatata
      841 aagccgagga acttgagaag acggtagttg tgtatgaaga acttgaatct tacggaattg
      901 atgcggataa aggattatat atgacgttgc tgaaagctta tcaaaaacaa aagactgaga
      961 aagtaaataa tttgcagaat ttgatatttg tgtatgtaaa aaactggttt attgaaaaag
     1021 ctattgcggc aaagcttgca aacgaacaaa gagaaacatt taacgaatta ccaacagtta
     1081 gtacggaaaa ttggctagag tagttgatca attttaaatt agaacttttt tataatttta
     1141 attttattac aaatgttaca aataaatagt tattattcaa aggttttaag attaaaagtt
     1201 tatgaaaaca atcacaaacg taggttttaa attcataatc gttagatatt ttagttattt
     1261 ttcattcaaa aaaataggga actagtgttt gcatatgtgc ttttttgagt tataataatt
     1321 catgaagaga ggtaattcac ttatttcccc aagctgaaaa gcctgatatt acttcttttc
     1381 aggcagtgct gctaatattc aattacactt ttttgtatat ttttagttgt tttaaattta
     1441 attgtctgct atgcttgtga tgaattgatt ttcgttctgc acttctggac gataaattgt
     1501 gataattgaa aatacaaaaa aaagaaaggc ccctgtaagg ctcgggagac tggtaatctc
     1561 cgtcagtgtc ctacaggaaa gcattaagat gacgccttaa tgcgtgccct tcagtgttac
     1621 ggtattaccc gcaacggact cagtataaca gatattcaaa agtgatacca ccttttttgg
     1681 ctctattaag gttaaaaata cgtgatattg taatgaatct atgcctaagc ataatgttat
     1741 aaaaatgtcc gtgtggctct ttgccgtatg acgaatgctg aaattagagg gaacgcatgt
     1801 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     1861 aataacaaga aaaacttatt aaaaatattt aaagaaagtg agaggattat tttatgtcat
     1921 atacaggaat tttgagtctc gaagatattt gtcattacgg taaacggtgt accgctacag
     1981 agaaaatcac gaagaaacta tcaactgggc aaagcaaagc tgttgtgcag tgtaagaaat
     2041 atataatcca gaaagacaaa gtttcagaag aaatgattta ttacactggg aaacgcaagc
     2101 aaataatttt aaaagatcct atccctttaa aagagctata tccaacaatc aaacatgttt
     2161 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     2221 aagggatggg gcgtttaatc agctgaatta atcactctca taacttgtat tttatcgttt
     2281 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagaatcat tagaaaaata
     2341 aggagtagac ttgatctatc aacaattcct ttcggggggt gacggggtag gtttttacga
     2401 ttggtaacgt aaaaaattgg tcaatcgagt aaatgccctt gtttggatag tggtgaaatg
     2461 aatttgtgta aataatatgc acagagtaaa caagagggaa tttagaagtt agacaagtta
     2521 gaatagcgcc tagattaatc atgagcagct actaagggat ctaggctttg acgcaaactc
     2581 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     2641 ctgcagtaca tgtagatact tgcgaagacg ttgatctttg gatcaatcga gcatttaccg
     2701 ttttctacta gggtttctcg tttcggacgc cataaacgag tcgatttatt ttatatattt
     2761 ttgagtgaac gaaaaacgat aaagatattc gttatgaagt aaaactaatt tagtagatag
     2821 caaaggtggt agaggaatga ttcaaaaatt tgcgaaaatg agtaaaactg catggattga
     2881 aaagcttgaa aatgacttga gtgtcatgcg attagctcca aagaaattca aacgagagat
     2941 tgcagaaaaa gaagcaattt tgtcggtatt gaatgcggta gacgataaat gtccagtaaa
     3001 aattagtttt tcagatatag caacaattat cgaaggtatt tattatggtg aagttgatga
     3061 agagatatct tctacaaaag atttattaga ttttttggat aaaaacaaag atacctttat
     3121 agtactaact gattatgagg aggttacaac aaatgaaaaa ttatgatccg aatatacgat
     3181 tgggcacaca tactattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt
     3241 ttagaagagg cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat
     3301 acgatcaaac attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag
     3361 gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag
     3421 atacttggtc atatttgagt gattatattg tcagcgttga aatcattgaa tttgtagcgg
     3481 ataaagaaga ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac
     3541 gacgattaat aaaaaggtag tattacctta ggcattgagt aaggaattta tttaggagga
     3601 aaattagatg gcgcgaccag ttttagaggc gatggatatg gatactttga caaaaggaag
     3661 gtatgtacag ctgttagctc gtggttattc acctaaagat gtattcaaat ccctgtctaa
     3721 aggaacagat atggaaaaag aaagattacg aaaagaattc gattattgga gaacccataa
     3781 tggaatcaag gatttgaaac cagcacggcc agtaattaaa cgaaagaaaa agataaccca
     3841 agactgaagt gtagagcgta tgtgatcata ggctttttta tttttgcaaa aaacaaggaa
     3901 ggtaggaaaa ctcatgaatt atgaagaaat cgaaaacaga aaaaaagtgt cgaaagaaat
     3961 ggaagaaaag ttgctaaaaa cgatgaaaca aaaacacctt aaaagattat ctgtcatgca
     4021 atatatcaat gatatgcaga taacaggaaa agagaaagct tgtttgttag gttcaatgaa
     4081 aaactttgaa cagttgagac gaacttatgt gaaaaaaagt tctaattgtc aactattgtt
     4141 agaagtctca tgacattcat taatagaatt catttttaaa actttttcta tggaaatgag
     4201 aaaaagattt tttgttttgt ctgaaagggg gtagctggat gagaaaatgg ttatttggtt
     4261 ttttaggagt cgctttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
     4321 atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
     4381 cacacgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
     4441 aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac
     4501 aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
     4561 ctttgagtct tggagcggct acttattttt atgaagatgc tgaaatgggc aaaggaaact
     4621 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
     4681 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
     4741 cgcgtaaatt tatcgtacct gggtacttta aattaatcga tggcgttcct gaagagaact
     4801 ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
     4861 atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta
     4921 atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat
     4981 gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca ttgcaggctg ctttcagggg
     5041 tatcaagtac atgcagaaga tgtcacgcaa aaaacaccac cagagaaggt caatatcacg
     5101 gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa
     5161 aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat
     5221 tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc gagagaagat
     5281 tctgatctaa ccaacgcgaa agtggacgag attgtacaag atgtagaaga caagggatct
     5341 aacagtgagt acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt
     5401 gagatcactt ttactgatca accagcctat gtcaattcca aagggaatgt atatgtggtt
     5461 tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt
     5521 gcgccaatga cagataaaac aggatcagga tttttaaaaa atattcattt gtatcctaag
     5581 aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cgcagaccaa
     5641 agcaaacaaa cccttttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa
     5701 ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa agctgactgc gactgatttg
     5761 acgttaggaa aatactattt tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa
     5821 gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc gcacaataaa
     5881 ttaacctttg aaattacgaa tgatggcgtg acaagtgact taaaagcttc atacgtaaac
     5941 tataaagcac cagtgatcga taaaacagta acaaatggaa caggacaaga acattctttc
     6001 caaattggtg acgcagtaaa ttatcaagga acgattcata ttccaacaga cattgcaggt
     6061 ggcgcagacg gtatcacagt aaacggtgtg aaaagcgaaa cttctcctta cagtgtattt
     6121 aaatggggag atacagcagg acaaggactt tcttatgtcg ctgctaaagc aaatatcaaa
     6181 gtaacaaata aagatggatc tgtggtatta aaagaaaata cagattataa aatccaaaat
     6241 tcagaaaatg gctttgtgat cgattttatc gtgaataatg gccaagtaag tgatactgta
     6301 gcaagtttac acggacaaga tcttcaaatg acttataaca tgtatgtaaa tgacagcgca
     6361 gctgttgcca atccattaac aaatagcgtg gatttcgttt atagcaacaa tccattcaat
     6421 caggaagaac accatgaaaa aacgaaagcc gacgctgtga cttatggcgc aaaattctta
     6481 aaagctgatg gcggactatt tggaacagga atcaaagcaa caccgcttga aggcgcagaa
     6541 tttgctgtta aaaacgcaga aggaaaatat tatggtggat tagttgatac ggataaagac
     6601 ggtgtcaaag aagcagtatg ggtagacgat gtagcaaatg cagcgatcct aaaatcagat
     6661 aaagaaggtc actttgaaat cactggttta actgaaggtg aatattcact ggaagaaaca
     6721 aaagcaccaa ctggttacca aaaattaaca gaagatattg catttaaagt aaacaaaaac
     6781 tcatttaaag aggaaaatcg aatcactgtt aaaaataatc aaaaagcagc tattccatta
     6841 acaggtagta atggtttcca aacgtatgta ttgatctctt gcttgttgct aggagcaggg
     6901 gcactttctg cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt
     6961 cgtaagcccc caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg
     7021 gcagtagcag tctatttaat tccatctgtg gaaacaaatg tagcgaaatt tcaacaacag
     7081 caaagaattt tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa
     7141 atagagttag gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca
     7201 atttatgacg gaacttccga caaaatttta gagaatggtg tagggattac ggaaggaact
     7261 ggcgatatta caggaggaaa cggtaaaaat cctttaatcg cggggcacag tggactttat
     7321 aaagataatc ttttcgatga tttgccttca gtaaaaaaag ggcaaaaatt ctttattaag
     7381 gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg aagaagttaa agcagaagaa
     7441 ttacaaaaaa attatttaga ttatctcatg cctgaaaaag accaagatag gatcacgtta
     7501 atgacatgca caccaaaagg tgtcaactca caccgctttt tagtgtatgg tgaacgagtt
     7561 tccttcagta aagaagattt aaaactacag aaaaaagcaa aaaataggaa atcaatttca
     7621 ttggattggc gattagcact gctattcgct tttttttgcg ttcctattat ttttatttat
     7681 aaaaaaataa ggaggagatc ggaatgaata agagaaaatg gctgtttgtt tgtgtagcag
     7741 ctctcttttc tactgcagct tattcgcaac tgtctttcgc tgacagcagt gatagtagta
     7801 ccactcaaac aatagataat tcaattactg caccaagcga aacggacagt tcggagaata
     7861 ctacagaaga aacggcagat tcaaatataa gctttactga atcaacgaat ccaacgtcag
     7921 aaagttcttc agagacttca gaaaagccgc aagacgtaga agatacgcaa gtgatcaagc
     7981 aagccattca tattcaaaaa attatttctt cgaatcaaat caacaatcaa ggcagtcaag
     8041 tagcccagca agaaggtatc aatggcgcaa agtttattgt ctacgatatc acgaatatct
     8101 tagaagaaat gtcacaagat gataatgaga aaaatcaacc tgtagaaata attgagtcaa
     8161 aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg gctgaaggag
     8221 aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa gtacctgcaa
     8281 acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt tcaaacagtg
     8341 aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg acggatgttt
     8401 ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa gttgtgtcaa
     8461 ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa ttattttttg
     8521 ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta tcctttttag
     8581 ccgcaacatt attgtgttgc ggtttaattt tttctcaatt atcaatttat ccaagatatc
     8641 aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta gaggtgatta
     8701 aaaagctttt gatcagtcca atttttatgg ttagtttggt tcttcttaca ggattggtaa
     8761 cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg tttaaaaaaa atattagatg
     8821 gagggcatta tggaaaataa aattcattgc actacgggaa gagtaattac tttagtgttg
     8881 tttttgttcc ttcttctttt tgcgagggga actgcagtcc aggccttaga aaaagttgat
     8941 ccgagtcaaa tcaaagaaaa taataatctg gtgctttatc atgatagtga ttcattgaga
     9001 aatatcgaag taactgggat caatggattt aagatactag atgagtattc aactaaaaaa
     9061 caaaaagtac ttgcttggcc aaaagtcaaa gatttgaaaa gtacgtcaaa aatcgttgcc
     9121 cattatcaaa aagtaggtac ttatctagga aaagaaattg attgtgacgt gactttttct
     9181 aattttgtag cgcacgctgg ggttaaccaa gacggattag ctaaaacaat taatggcaaa
     9241 aagtattata attatttgct ctttgataaa aatatttatc gaggattttt catgcagtca
     9301 atcaatgtca tgaatgcaaa actagaattt aagtatgcag gaacaaaaac aaaagtagct
     9361 ttgagtgaac cacagccaga aattcaatca aaaatgagat cgatgagagc agctggtatt
     9421 gatttaaacg atacatttct atccatttat agtttaaatg gacagtatca gcaacgtgtg
     9481 tcttggtata atgacaataa tgaatctgtc ggtgaacgta atattgatat gagagaattt
     9541 gttgggtatg aaaaaatggg tagcttacct tattttgtca caacagatac agcatgtgca
     9601 gaatacaaag ctcctgcgtt atcaacaaac aatttaactt caaaagtagt gggaggacgc
     9661 gcagaaaagg cttatagctc gaatgatcat ttcaccgatg ttgtaggagc tgatacttat
     9721 cacagaagtg gtgtaacgta tacgcttcaa ggcgcttccc caacattcat gattggcgca
     9781 aatacgaata gtatgatgtt tagctttgat actgcattgc tatggacacc acaaccatcg
     9841 aagcctacaa aagaagtgtt taacaaagct aatactgaag aggcagcaca caatattgac
     9901 aaaaaagtga ttccacaagg atcagatgtt tactatcata ttcaccaaaa gtttgatgca
     9961 ttaacagtca acacaatgaa caaatacaaa tcatttaaaa tcactgatac ctttgacaac
    10021 aaaaattttg atatggtatc ggatggggaa aaatatgatg gcgcattgtg gtatgttgaa
    10081 gcgaatggta cttggaaaaa agctgataca ggaggaaaat taactgtatc aggtaataca
    10141 gtcacttata ctgcttctga cagcttttta gctaacgctg caaattacgg tagggattat
    10201 attttgcagc tccatatgag agcaaaaaat gatgcaaaag gaattgcacc aaatgtggct
    10261 actgtaacgt ttaacaatga tccgaacaaa ggaacacaaa aaacaaatac agtagaaaac
    10321 tatttctctc ttaaaccaaa taaaaaagtg gaacaagatg ggaaagatgt tgatggtcga
    10381 aatgatggta aaaaaggaac gcctacagct cctttaaatg caggatctga agtacaatat
    10441 ctagtcacac aaaaatggca tacaaaaggt gtagacattg cctcagatca ttacaagcaa
    10501 tttagtattc aagatccagt ggaagatcgt ttgacgtata aagaaggttc tgcgcgagtg
    10561 atagatatga gtacaggaaa agatattact tcagaaggta cactaaccta tgatagcaat
    10621 tcaagaacgc tgaagtggga agcttccgct gatttcttaa gtaaaaatct tttagatgga
    10681 cgagaaattc aactgatatt tacagctaaa actcctttga atgaagaatt ggatatcgac
    10741 aaccaagcaa aagtttcagt agaaagtatt gagaaagcaa ctaatgtagt aacgattggg
    10801 gtaaaaccta acttacctca gatcattgtg ccaaaaactg gatcgaatca tttagtgcta
    10861 actgttattg gaagtatatt atttcttact ttagcatcat tgggctatgt agttttaaaa
    10921 ctcaaataaa tataaaaaca aggctgagaa tcagccttgt tttcttttat ttgaagattg
    10981 gattcgcact aataaataca ttatctacgc caattctacc agcagagtca actacaaaat
    11041 tatgacctaa tccgagaata ctttggttat tataaagtaa atttgcatta tgggcagggc
    11101 tatttttcca tcctaccatg gcatcgatag caccatcgtc tgataaaagg tcatcaatat
    11161 acagcccagt ttgacttatg ttttctccct tagcaccgta gcagcctaag ctttccaata
    11221 aatcttggaa agtaccacca ccagggcgat agtgactgaa gctgacttgt aattctttag
    11281 ctcgaatatc tgcagcctgt tgaacaatat cattgaattg cattggggca acaccatttt
    11341 gttggcgata atcatttaac caagcaagtt ctttttgacg cataaagtta cgtaattcgt
    11401 ctaatgaaat ctttttatat ttgaatgtga ttgttttatt ttgatcgacg ttattaatgg
    11461 atcgttggcc gtttttatcc acggtaacgg taggatcgtc atttctgcac aagaaataat
    11521 tttgatcagt catgtttttc caaggagtag caaagctatc cccttcgtat actgtttttg
    11581 attcactaga gagaacagcg ttggtatcag catttacatg attaacagtc acattaaatt
    11641 tacgagtgta gttgaaaata taattttgac tttcagtcac attgtttaag gttactgaat
    11701 ttgttggttc tacccgatcg tctaaaacat atccttgaat agaaggagca gttaaagtga
    11761 cactgtcacc gtgagtgaca ttgactatct gttctttcaa aatattaact cgtccttcgt
    11821 ttttgtagtt gttattgcac aacaaattga tttgatgcac ttgattataa ttgaatgtga
    11881 ttgttgtatt acctgtgatt gttttgcttt gagtatcatt accaatcact ttgtagctaa
    11941 aattatcttt agcgccttcg ctatatttaa atgcttttgc cttagcagtg taagtagaac
    12001 cagaagttac ttttgcttga tctgtagcaa tcacttgacc tgtttgattg tcaatatggt
    12061 taatagtaac cgtatattct tttggtgttt caggtttgtg ttgcaaccct ttaaatgcag
    12121 catcaagact atttttagca ttatttactt gggcttgaga agccttagaa tcgtctagta
    12181 cttttttagc attggataaa gcagtctgaa aattattcca agattcttct gtaaaatctc
    12241 ctttagcagt tgattttact cggttgtata aagcttgtaa ttcagcttta ttcacagtag
    12301 gaactggttt tttctttaat cctttgtaag cagaatctaa tttatttttg gcactatcca
    12361 cttgtttttg agtcgctttt gagtcattta acactttctt agcattgttt aaagcggttt
    12421 ggaatgcgtt ccaggtattg tcggtatagt cacctttttt agttccctta acgctcgtat
    12481 aaagagcttc taaagctgat ttattcactt tgggtttaga ggtgtcagcg ccataccaac
    12541 cgataccttc gtatttccag ccatagtctt tggttaacca ttgtacttct ttccaacttg
    12601 cagtgtagtg atgtgagcca gattttacac caggaaggaa aaggcggtag atcggtttat
    12661 tatttttttc ggcagaacgc caggcaggcc cttcataagt ccagccgtaa tttttagtca
    12721 accatttcac ttcatcccaa ctagctgtgt aatggtgatc acgaagacct ttattgtaca
    12781 aacgatagac aagatggcca ccgctagtcg gtgcttccca ggcggggcct tcataatttc
    12841 cccattttat ctttactaga tggtcacgtt cttttttgct agaggtgtaa aaatgttcat
    12901 gattatttgg attgtaaagg cgatacatga cgttcgttga agcagcgtca acagatccag
    12961 caagactaag tgaggcagca aatgttccaa tagctaaaac agtttttgga ttaaataatt
    13021 tcattttttc aactcctaaa tttaattaaa aatgtttaca tgattattat atatcggcta
    13081 accgatatag tcaataaaaa ataagaaaag ccttatataa ttttggcttt tcttatttta
    13141 tttgttagtt gaagtattaa tatcaacgat ttcttcagcc aatttattta atttcatagc
    13201 atttatgaat ctcatgttgt ctatactaga gcgtttgttg gcaaggtcac ttactgtagt
    13261 taagggtacg ccagattcta tggatataga acgaactgat ttaccactat caaacagcaa
    13321 ccattcgatt ttctttttat cagcgtacat ttcaatgctc cttcttgttt tttctttatt
    13381 atagctcttt tttgtaataa atcacaatat caagagaaaa attttctata gaaaggtgga
    13441 tttttatgag tattcgttct ccgtgtatcc aaatgaggtt tgttgctact ttcgttattt
    13501 gatatactaa aaataaacaa cgaaagaagg aataaaatga agaaaaagca actatggttt
    13561 gtctctatgt ttgcaatatt ttttattatt ttaagtggat gttcaagtaa taagaaatcg
    13621 gaagctaaag aagataatag tttattgcaa ccaggaactg tttggaaaga agaagtaggt
    13681 ggtcttattc ataatttaaa aattatagat gatactacct gggaatattc tgaaagtgta
    13741 tggcatccag atcctgtaca aataacagtt aagagacaaa aagactatga agggttagaa
    13801 agatacaaaa tagtagattc tgcaggtatc agaaaattta taaacaaacc cgattcttta
    13861 tttatcgttg ttccttacga gaaagatggc gtaaaaaaaa ttgtttttct tggatctagt
    13921 aaggatgaaa agcgaacgaa agaaaaattg attcatgatg gatcacaatc aaataaatac
    13981 aaattacaaa agactagcga gtgattgcta gtctttttat ttaccctaaa ggaggaatca
    14041 tagtggcaaa agcgaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgtcgcg
    14101 gtgtatctga aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac
    14161 ggggaaaaga caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt
    14221 tcgatgaaat tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat
    14281 gaatttttta acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac
    14341 tgccgcaact agtttgaaaa ataatctgat gatttttgct gctgttgcgg cagtggcttc
    14401 cattgctctt ggaggtattt tcttcttctt tgggcgaaga ggagctgaaa acgggaagac
    14461 acacgtaggt tatgtattgt tgggggtagg agttatttta gctgccgctt ctattgtagt
    14521 ttcaatgttc tcaatgtttg gacaaacccc acaaggacta taaaaaaaac aagtagaagg
    14581 agtaaaaagt acgtggctac tttttactcc tttttttatg aaaggagaat attcatgttt
    14641 tttacgattt caggaaatgc aaaattggat aaagagttaa taagaaaagc aacgaagaaa
    14701 gtgggaaagc gtacttcctt tttaccgaaa acaattaaat attctgtcat tgaaacggaa
    14761 gaacagctac atgtgttagg acaagtgtta gaacaagaga atcaatcttc tgttaaattg
    14821 actctcacgt tacatgataa agaaggaaaa atagaaaagg ctggcacaga atacgttttt
    14881 ggcccactgg attatcaaat ttcagaaggt ttttatccta cattgatgga tttaatccgc
    14941 cttgatgtgt ttaaagatac ggatgaaact atgagtgtgg aagagtctta tcgaaacgaa
    15001 aaacttttac aagttttaga agaagagtta gagagcgacc aaagaaaagt gaatcaagtg
    15061 agtgaagtcg aagtaaatga agaagtacca tgggaacgac caatatttcc aaagaaggag
    15121 tcaaaagaaa aagagacagt ctatgttgac gaacaagctg atattccgac tcagaaatcg
    15181 atatttgagg aagagaacga tgtgatgggt caattggagg tagatccctt tgaagaaaca
    15241 atggaggatg aagagacgga cgaagtagaa gttgatccag ttgaagctaa tgaagagtca
    15301 acatggactg aaggggattc tcaggaatat attgaaccac ttatcagtga agaagtagaa
    15361 aaaaacgaaa cagagataca aaaggcaact gtatttgatt atattttgga tcaatatccg
    15421 ccagagcatg aatggatgaa agaaaaaaac aaattatttt taaatgagct ttattcgaaa
    15481 tatcaattac cccaaaccta tcaagcattt ttagagagta aagaagaatt aatctcacag
    15541 ggtaaaggca cacttttaga acagttagag tctgtccaat caacaaattg gcatgagctt
    15601 gcacaagaag atttattagc cacctttgaa gaacgtcaac aagaaacaga tcaagaaatt
    15661 aatgagtatg cgacggaaca gtcgtctaat tgggagaaag taaaagagga actaaataaa
    15721 gaagaacagc gagtgattga agaagaagta caaaaaatca aagagcggta cgaaaagaaa
    15781 cgcgacaatg cttattcctt gtgtgtagaa aagatcaaca attttactaa gacgaatcaa
    15841 gaagcgttat cgaaagaaaa agaaaacttg ttaaaagaac gtattaaaga acgaaaagaa
    15901 gagttctacc gcggtttacg tacagataag ctaaagatta gcaaagaatt aaatgaacaa
    15961 ctgaataagc tatatcaaac aacgactgaa gttcttatgg aaaaacacga acagatccag
    16021 caagaattac aagtacaaat tccaaaatgg aaaaaagagt atgaaattca acaacaagaa
    16081 ttgaaagaac aagaagaaaa acaacggcaa aaagaaaaag agcgtgaaca attagaaata
    16141 agaaaacggg aacaggatct tgcggaacaa aaaatggcga tagaaaaaca acggttagag
    16201 caagaaaagg aaaaagaagc agctgctcgt aaggaacgtc aagaacaaat ggatacattg
    16261 cgtcaagcac agttgaatta tctagcacaa cctcaaccag tagctgctgt tcaaacgcca
    16321 gtgaacaacc aagcaaattt aactccttca ccccagcgtt cctttaacgg atggatggtg
    16381 ggatgtattg caagtttaag cttgcttcta ggtggaggaa cagtatttgc ttttaatcat
    16441 atcaatcaat cttcacaggc tgattctgta gccacaacgg cgtcaatcaa ggaagaagcc
    16501 caggaacaag caagaaaaga gtatgaacaa caattagaag aattattgaa tcgtaccaat
    16561 cagtcaacaa cagagtcaag catggaaaat agcaaagaga gtgattctag taaggctaaa
    16621 gaaggatcaa gcgattcaac tgtacaatca aacacttccg aaagttcaga atcaaatgag
    16681 gtgaaataat gcgtgttaga gaaacaacga agcgaaaaga caaacgattc aatttagaaa
    16741 agaataggta tctttctcat ttctatcgct atactccctc aggagaagag ctaccagaaa
    16801 aacatgacaa acgcatttcg aaaaagattt tttatagtgg acttgtatac ctccctttag
    16861 agtatttagt aacaagagta ccagaagttt tcaaccgatt ggaaattgtt aagggaagct
    16921 tacaaggaat aaatctgttt caattacagg aaatgccaca ggtctttcat tttgattcag
    16981 ctggtttttg gaatccattt actcctacaa ctagtttggg tacacgttta gcaatttcga
    17041 cagtttgtgt ggttccagct gtacttatgg gattaaaaat ggacatgtgg tttcgtcctc
    17101 tagcagatgg acaaaaaggg gataatcgat taatgacctt aaaagaaatc caacagattt
    17161 atccgcaaat acctgaaaca aaaactactt ttccaggtta tggaggtatt ccaatcacac
    17221 attacaaaaa atattggtac attcaaacag atactgttaa tacgtgtatt gttggtacct
    17281 ctcgatcagg gaaaggtcag attgaagtgt tagccaccat tgacaattta tctcgtgcag
    17341 aaagacaaag ttcaatggtt gttaatgatc caaaaacaga attatatgtt gctagtaaag
    17401 atgaactaga agctagaggg tatgatgtgt atgcctttaa tatcaccgat ccactgcaat
    17461 caatgagtga taatccattg gcactcattg tgaaatactg gaagcgagga gacatagaca
    17521 ccgcaacaca gttaaccaat acttttacga atacgattta ttatgatgca aatgcttcag
    17581 acaataaata ctttaacgaa aatgcgcaga aagctgtcaa tgcgcttatt tttgcgttat
    17641 tggaacaagc ggatcaaaca ggggattact ccaaattaac accaaataat ctcgttgaac
    17701 tactttcaga aattggcggt tttaattatt atccagatcc tgagaatgaa ttcaagcagc
    17761 tcaatgcctt agatgagttc atgaatcagt taccccctgg aaatatagca aaaaaacaat
    17821 atggggcaac aaaaattgga tctgataaag cgaaagggaa tatcttgtct acggcaatta
    17881 caggattgaa tccatttacc ctaacgaaaa ttgcaaagat gacttctcaa tcaacgattg
    17941 attataaatc ggttgggttt ccaaaatact tagatttgaa attacatgaa agtcttttaa
    18001 atcaacgagt cgtagtagaa ttttatcata agggaaagaa atttcatgaa gaacgtgtca
    18061 aagtaggcta taaaggattt tgtgaaatca attttgattg tcatttagtt gcaggtgatc
    18121 aagtttgttt aaaatacgat catcaaggaa aagaatatgt ggcttggtat gaattttctc
    18181 aaagagtatt gaaagacgaa gagggtaata tcgtttatcg aaaaaaacga ggagaaaccc
    18241 atttgccaga attagaaaaa gaagccgttc taacattaga tgaggataaa agtaacttat
    18301 tagtagagtc tattcggatg cactataccg ataaaccaat ggctattttt atgcttacac
    18361 ctgattatga tccatctaac catatcattg ttagtatttt tcttagtcag ttatataaag
    18421 aattatctac gcaatgtgtt aagactaaag gagataaatg tcaccgtaga attcatttta
    18481 ttttagatga atttgggaat atgccaccaa tggataatat ggaaggaatc atgacggtca
    18541 cagctggccg aaacatgttg tgggatctgt ttattcaatc ttatcagcaa ctttatgcga
    18601 agtatggaga acaaaacgca gcgattatca aagaaaattg tcagacacat atttatatta
    18661 tgtcgacaga tgataatacc atcgaagaat tttctaaaaa ggtggggaat aagacggttg
    18721 agcaagaaaa cagtaccatc aattcattag gtatgaatac tcatatcaat cgcaatgttg
    18781 attcagatcg tatcttaact cctgaaagaa tttctacttt tttagaaggt gaaacgattg
    18841 tactcgctcc tttaaaacga caagatttga aaggtcataa aatacggccg tatcctattt
    18901 tcaatcatgg ggaaacgaat cttccttatg cttatcaata tattgaagat ttcaatccat
    18961 cgtgggatat caatgatata gacatacatt gtacacatgc ggacttggat cttaaggctt
    19021 tacaaattga ttatgattat ttcctaccag atgaatatgc tagaaatctc tatcatggac
    19081 tgcatgatca attagaagaa aaaaacgaag aaaatatgtt ttataaaatc gttcaatctc
    19141 ttgagaatga gacggctaaa aaattgttaa ttcaatttta cgaagaacaa gcagcagatc
    19201 gacttcaaga atatgttgaa aagttgatca aaaataaaga aatttctgaa atgattggga
    19261 aacggttgtt attggaatct gaaaaggtgg tgaaagaatg atcattggag gaattttttc
    19321 tggcatagcc gactggtggt caggcttatt cggagaaggg gatgcaaacc ccaaaaccgt
    19381 aacgaaagtc ttacaagatt atggtaatta tctgcagtat caagatgtat ttagttggat
    19441 ttggaacact tttctgtggg gattgattaa aggattatat aatctcaata ctttactaga
    19501 aaaaaccatt tatcaaagct ttgatttaaa agacttattg aacgccgctg gggtaaatgc
    19561 actatttcaa agtttgatta gtaaggtatt ggctcttttt tgtgtcgtca cattaattta
    19621 tttaggcttg aaatttttta tgagtaaaca cccaccaaaa gtcaaaaata tcttggttaa
    19681 tttatttatg gccatgatct tgatttttgg tggtagttct ttaatcgatg aagggttaaa
    19741 tatcagtaaa atcttttacg gagacgtcac tgcagcaaat aaagcagata aaacgggatc
    19801 acctgctttt caaattattc aaaataatgt gatcgatgtc agtacgttac tagcaactga
    19861 cccgaataaa gtagaatccc taccaacaga taaaagaaat gctttaacag cgaaaaattt
    19921 taaaacagcg aatatcaatg ggattattac tcctgatcaa gccgctgaga tggcggataa
    19981 cgataaaagt ggattgtcag atttacaaaa ggatcggtta aaggcattac aataccgatt
    20041 ggaactagat gataatggga aagaaattcc tgttcaaatt agtgacgaag gcttagcaaa
    20101 atatgtctat acttcagggt atcgtcgata cagtagtaaa ccaggtatta tcttagctgg
    20161 tgaatcttct ttggcggtgg cctatttatt tatcctattt acgatcgtta cttgcattat
    20221 tgagctggta tttaaaaaat tctatttagt gattgccgcg tcaacggact ttgagacagg
    20281 ccaacggatg aaaagtgcga tagaagatat ttctcaatcc ttcttgttat tggcgtttac
    20341 gattttagaa ttacgaatct atacattaat gctttcaggt atcggtgatt tgcatgcggc
    20401 aggaaagatc aatggattct tgtatgttgt tgctttaatt gtgctaacta ttgcgctatt
    20461 caaaggctct caatcggtta cgaagatttt cggtgttgat acgagtctga aaaacggcag
    20521 taattctctt cttagcatgt ttgccttagg aaatattgcg aaaaatgtgg gtagcggagc
    20581 taaaaacctt gcaagtgctg gtaagagtgg tttaagccat ttaaatgata ttcgtaaaaa
    20641 tggatttggc catcgaaaag atgttacccc tgataacggg gaagaacaat cagacgataa
    20701 agctacaact gtacctaata gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa
    20761 tggtgtttct aaagccgcag aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac
    20821 agctgaagat cttacagaaa aagccaaaga caccttagaa gagaaattag acggcacgag
    20881 agctaaagaa ttggctgaag ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga
    20941 aaaagcttca gaagtcaaag aaggactaaa agatgcgtat ggtgaaggac aagttaatgc
    21001 agcagtaaaa gctaatacaa caaccgaaaa agaaaatcaa gaaggtatag tgattccttc
    21061 agtcaaagat caccaagaag cgttgcactc taatgaaaat tcgattgaag agaaaaattc
    21121 catagaagaa agaaattcag aagaaggaaa aaatggacag ttaccaaaaa ctgatccaga
    21181 acaatatcgc tataaaccta acagtgacct tgaaaatgag aagcgaattt cagatttatc
    21241 aaaagtaaat caagaagaca aagcagaaaa tgagggacag ttaccaaaaa ctgatccaga
    21301 acaatatcgg tatagatctg ttaactccga aaatgaaaaa cgcatttcag atttatcaaa
    21361 acgaaatcaa gaagtacaaa cggatgagga ttccatcgtt ctgccaaatg ataatggaat
    21421 cgaagttgga actgaaaaat caacgaaaat tggtttacct aaggcacaaa atttaccaat
    21481 gaaagatagt cgtttgaatc aattcaagga taatgatcaa agttttgttc atcaatcacc
    21541 tgcaggtact gttcaaatga aaccaagtcg tttgcctgaa aatggaaatc tgacaactgt
    21601 ttcttacaat ttatctgaaa gtggtgagac agggaatgcg tccgttcaaa atgttgtgac
    21661 aagagaagct gcaccaaaac ctattcaaaa cgttcataca ggttacaagc cagcaaatgt
    21721 ggcatttact gcaccaacaa aaataaatgt gacaagagaa actaaaaaca ggaacaatgc
    21781 attgaaagaa caactattga gggatatcaa acaggatatc aaaccataaa aggcctacaa
    21841 gttaggcctt ttctttatat gaaaaggaga aatcaattat ggataaatat ataaacatga
    21901 aaaaccctcc aagatttagt ggggaatgga agtttttcgc ctttttcttt ttggatctat
    21961 taacgcttgg tggttgcttt ttttgggcga aaatagtcaa taaccttgtc catttaccac
    22021 tcttttttgc actcttgaat tatggcttat ggattgcgat agctctagtg attgtttggc
    22081 gaccctataa taacccaggt caacgacaaa tttttgcgat tatcgagtct atttttttta
    22141 cggacaagaa ccattatcat gcgttagatc ctaaccgaaa ttatcatggt acgtataagg
    22201 aggatgaata aatgcgagtc aaaggacaaa atttagttga taagaaatcc atttttcagt
    22261 ttggaaaacc agaagaacga tctatcttgg atttatttcc ttataaatcc tgtacagaag
    22321 agggagtctt agttactaaa gaagatcatt ttcaacgtta ttatcgtgta gtttctacag
    22381 acgtggaagg attaaatgaa caagagaaac tagaacgaat gaatcagttg accaccgtca
    22441 tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg gatttaacgg
    22501 aacaaatcgt gcaaaagcgg caattattag ataaaaatcg tatggaacag tccttaggaa
    22561 gaaatctaca acgattaaaa aaatatgaaa agaaattagt cgaagaaatc caggaattga
    22621 aacgagcaga acaagaacgt cctgacttaa gcttcttttt cgtcattgaa ggaaagacaa
    22681 tcaaggattt acaaaccaga aaccgtcagt taatgcgatc tagtggcatt ttagggttaa
    22741 aaccactcta caaaaaagaa ttgatcaaga ttctgtatcg aatgaacaat atgaatgatg
    22801 agtaggaatg gagaagagag aatgaaatat ccgttgatat ggttagaaaa agatgttccg
    22861 aaggaagaga ccactagaaa atttcctcac actttaagcc tgggtaagtt gggaaaaggg
    22921 caatctttta accaagtaaa gaatcaaaca agaagaggtg actgtacatg ttagaagcaa
    22981 ttaaaacagc tttttcacca caaaatgatc aaaaagtact tgatccatta aagcaactag
    23041 gaaatcggga aatagaaatt ttaaaatcca aagggtatga tttagatttt ttaaaacagg
    23101 tgatgccaca aggtgggtta aattttgatg aagaatatgt gacgtatggt aatggttatt
    23161 cacgatgtgt gacagtttat cactacacaa aaaatcctac gcttttttgg ttggccaatc
    23221 tcatgaacaa tcctcataca gtggccacac tcgatgttag cacagatgaa aagcaaaaag
    23281 tgattgatca aatcaaccgc gcattagatg aattaaatga tcgatcagat aatgaacgaa
    23341 aagcgactga ccgaaataaa agtgcggaag aatacctgga attagcggaa tatgcccgaa
    23401 aattgacttc taatggggaa atttctaaac aagtcaaagt gagagtacat gtttacggaa
    23461 acacgattca agaagtagat gaacgagcgc aggaactgat cgaaaatctc aaagggatgg
    23521 attatcgtgc ggtgacttat ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca
    23581 tgttcgatga acaagctgaa tggttcggaa atatcaaagg gcaatcacta ccatcgttaa
    23641 acattggcgg aggtttcccg ttccaccacc aaacgctaaa agatccagga gggacacaat
    23701 atggggaaac catgacaggc ggcccatttg tctggaatgc gacgtataaa aatagtgtac
    23761 ggttgtctac taataccttg cttctaggaa tgatgggagc tggtaaatcg accgttctta
    23821 aaatgatcgc ggaagcccat ttagctgcag gagactttat ttggggcttt gaaaagggaa
    23881 aagactttat tcctttctta aaggaatata acggtattat ggtgcgttta gacggatctg
    23941 acggaatgat caatccttta gagatatttg ccacacggac gtatgatgaa gcatccagtt
    24001 tatatgacga tggatcagtc aaagatttga agatcaatga agcagcgtcc tatcaaacac
    24061 acttagacaa agtggtctat caggtacagt tagtgagtcc tcagctaaaa ggaacgatga
    24121 aagcagaatt taagacctac ttaaatcgtt tttatgaaga gtatggaaca gtaccacgcg
    24181 gctttacttc ctcaaattcg cgttcaaata ctgatacgca agtcacaggt aaagatccag
    24241 aagcttatcc gacgttcaaa gaatttttag actttttagg acaactggaa ttaccaggtg
    24301 cttcccaaga gaaaaagaat cgaaaagaag aaatggaatc catcgtagaa tccttatgtg
    24361 agacgtatgg catgattttt gatggtcata gtacgattcg acaccttgac caacaacagt
    24421 tggtcttttt tgacattgac tctattacag gcttagatcg aggggtacaa caatgtcaaa
    24481 tgtacatggc tatgacgttg atttggaatc aggcattgat tcaaggaaga agacaacgac
    24541 gtttaattaa agaaaaacaa atggaagaac aagacttcaa acgcttcttg gcgatgattg
    24601 atgagtgcca taatgcgatt aatccagatt ttattgaaac ggtaaattat gtcacaaact
    24661 ttcaacgaga aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag
    24721 aaatggtgcc agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat
    24781 tttcttctac gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat
    24841 tagtgggtaa atcaatgacg aactcagaat tagaatccat tcctcaacaa aatcaaggag
    24901 acgctgtgat caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac
    24961 gtcaattaaa attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa
    25021 gcagtatgtt aagcgtcgtg caaagcggcg cttatttttg tggttgtttg gtacttctgc
    25081 aggtttgatt actattttga tcactgtgtt cgtgacccta tttctaatac ttgcggcggg
    25141 ctcgattgat aattcggatt cagattcaag cagtggtggc gaagctttta caggagaata
    25201 ttcggaaggc ttaccgattt ataaagaaat caaaggaaga ggcccttttt ctgatgagat
    25261 tgcgcaatat gccgtaggtg cagcagtgaa atataaatta ttaccaagtg tgatcctttc
    25321 gcaatacggt tacgaatcag cgtttggtac atcggcgtcc gcaagaaatg atctcaacta
    25381 ttttgggatt acttggtttg atggttgtct gtttcctaaa ggaactgcac gaggaattgg
    25441 tggcattgaa ggtggctggt acatgaagtt tcccaactca aaagctgcat tttcctatta
    25501 cggctttatg gtagccacac aatccaattt taatgcttgt gtcggaaata aaagccctgg
    25561 cgcaagctta ttgatcttag gacgaggcgg ttatgccgct gcaggaatta cggaagatag
    25621 tccgtattat acaggttgta tgtcaattat cacttctaat aaattaaccg aatatgatga
    25681 attcgccatc aaacattggg gtgaaggtgg caataacaac ggcacgatta ctggtgaatg
    25741 gacgaatcca ttccctggca gcagtttaga taaaagttcc ttcagcggtg gtcaattatt
    25801 tggaacgaat ccaggcggtg aattccgtcc gaacggcttc catgatggat tagattttgg
    25861 ttcagttgat catccaggaa gtgagattca tgcggttcat ggcggaaaag tcgtgtacgt
    25921 tggcaatcct ggtatttcag gtttgggcgc gtgtgtgatc gtgattaatt acgatggctt
    25981 gaatatggtg tatcaagaat ttgcaaatag tacaggaaat tctcgagtga aagtaggcga
    26041 tcaagtgaaa gttggtcaag taattgggat acgagataca gcacacttgc atttaggatt
    26101 tactcgaatg gattggcgtc aagcacaagg acatgcattc attgatgatg gtacatggat
    26161 cgatccgtta ccatttttga atagcagtaa aaaatagatc aggagaaaaa atatggaaaa
    26221 taaaacgtta aaaatcgtct taacagctgc aattagcgtt agtttaatcg gcaatattgc
    26281 gttgttttgg cagctgaaag aggaacaagc agcaaatacc tataaaattg aagaagtggc
    26341 taaaaacgcg tctgagaagc tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa
    26401 gttaaatcaa accatcaatg agaaaaacca agaaatcgat cagctgaagc aacaagatgg
    26461 cacaaaagca aaagaggaag taactgaagc acaaagaaag acggcagaag agttcggtca
    26521 attggccatt gataaatcat taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc
    26581 aacccaagaa gtgatcgaca aattaagtcc tacagacgat gatcaccaac atgatgatta
    26641 cggaagttac tcctttacat taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa
    26701 agaggaacaa aattttgtcg tatttgttga ttatacaatc ggcaatccgc aattcaaaga
    26761 tgtcaaacca caaaaaataa aaggtggatt aactgtcact gaaaaacaag tagatggtca
    26821 atggaaagtc atagacttta gttatttcac tcgttaggag gaagaaaatg agtaagaaaa
    26881 aaggagaaag acaacgtttc aaaaaacgaa aaagttatct tcaatgtctg ggaatcttta
    26941 tgattctcgg ctttttgatt cttgctagtc cgatttatac gaatcagaag gtagatatac
    27001 cagatgaggt aagcacgaac gcctttttaa acacgaaaga gctgacaatg gtttcaaaag
    27061 aaaaaggagc gaaccagcaa ttagtttttg aatttgcgat cgatgatgaa gatcaaagct
    27121 tgttaaaaga actggctaat cttaattatc gcgtagaagt aaagacagct aaaggggatt
    27181 atcaagcaat acgagccaaa gtgattaagg tgtctgatga ttattttgca gtgaagctaa
    27241 cgcatgtacc agaagaatat atcgccatgc gtttaaccat tattcctgaa aaaattgatc
    27301 caaaagtgga tatgcaagaa ccacaagatc tgatttatta cgttcacgaa gataaggtta
    27361 aagatcaagt gaaagatgaa gattataaac aacatgcgat caattacaaa gtgaaaggat
    27421 acaaaaaaga acaaaaagca gcacaaaaac aaatggaatc cttccaagca accattgatt
    27481 tgaatgaaga attagtgaag aagctaaaag atcaattgcc ttatcaagtc gcagatgatc
    27541 aggaaaatac taaaaacaag attaatagct atcagcaaga aattgaaaca tcgaaaacgc
    27601 aactgaaaga tcaagaagag atcattcaaa aacttcaaga aaaaattgat cgtatgacaa
    27661 aagaaaagat ttagaaggac aggtggagtg aaaaaatgaa gcgaacaaac ttggctgaca
    27721 aagatacttt cgctgagcag aaagtattga ttaaagccta tctcaaagtt gtaaaagcta
    27781 aagaaatatg tcaagaagtg aatttatcta acacaacaat atctgagttg aagaatggta
    27841 ggaagagttt agatacctta aaattcagat catttgaagc tctatatata gctgcaaaaa
    27901 aa
//
LOCUS       DACDVT010000030        27640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDVT010000030 DACDVT010000000
VERSION     DACDVT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.030"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="IWL52_001359"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="IWL52_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9777931.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="IWL52_001360"
     CDS             complement(1195..3036)
                     /locus_tag="IWL52_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9777932.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5611)
                     /locus_tag="IWL52_001361"
     CDS             complement(3218..5611)
                     /locus_tag="IWL52_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9777933.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="IWL52_001362"
     CDS             complement(5718..6578)
                     /locus_tag="IWL52_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9777934.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="IWL52_001363"
     CDS             complement(6637..7269)
                     /locus_tag="IWL52_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9777935.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7478..8197
                     /locus_tag="IWL52_001364"
     CDS             7478..8197
                     /locus_tag="IWL52_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9777936.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="IWL52_001365"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="IWL52_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9777937.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="IWL52_001366"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="IWL52_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9777938.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="IWL52_001367"
     CDS             12557..12913
                     /locus_tag="IWL52_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9777939.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13154..14158)
                     /locus_tag="IWL52_001368"
     CDS             complement(13154..14158)
                     /locus_tag="IWL52_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9777940.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14324..15175
                     /locus_tag="IWL52_001369"
     CDS             14324..15175
                     /locus_tag="IWL52_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9777941.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15192..15437
                     /locus_tag="IWL52_001370"
     CDS             15192..15437
                     /locus_tag="IWL52_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777942.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            15624..16328
                     /locus_tag="IWL52_001371"
     CDS             15624..16328
                     /locus_tag="IWL52_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777943.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(16658..17863)
                     /locus_tag="IWL52_001372"
     CDS             complement(16658..17863)
                     /locus_tag="IWL52_001372"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9777944.1"
                     /translation="MEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIPMGIAQN
                     FQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKSEYQAVI
                     NAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDVKDAMGA
                     NMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNKEIGEQI
                     AKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAARNGLYQ
                     GLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQVIAAVG
                     LAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKMNQQTAI
                     QILEKIREK"
     gene            complement(17863..19098)
                     /locus_tag="IWL52_001373"
     CDS             complement(17863..19098)
                     /locus_tag="IWL52_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HAP9777945.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKSFISLRHLNGAVN"
     gene            19283..20437
                     /locus_tag="IWL52_001374"
     CDS             19283..20437
                     /locus_tag="IWL52_001374"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9777946.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(20459..21211)
                     /locus_tag="IWL52_001375"
     CDS             complement(20459..21211)
                     /locus_tag="IWL52_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9777947.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(21575..24241)
                     /locus_tag="IWL52_001376"
     CDS             complement(21575..24241)
                     /locus_tag="IWL52_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9777948.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            24583..26034
                     /gene="cls"
                     /locus_tag="IWL52_001377"
     CDS             24583..26034
                     /gene="cls"
                     /locus_tag="IWL52_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9777949.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(26239..27054)
                     /gene="thiD"
                     /locus_tag="IWL52_001378"
     CDS             complement(26239..27054)
                     /gene="thiD"
                     /locus_tag="IWL52_001378"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9777950.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            27348..27623
                     /locus_tag="IWL52_001379"
     CDS             27348..27623
                     /locus_tag="IWL52_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9777951.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         8050 a         5763 c         4886 g         8941 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaacactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctccgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctccta aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgtc atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggacttatgc
     8401 tgttgattgg tcttgtctct gtgttaccta tgattggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttact tgtttgagac aagctggctg atcaatacaa
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattccg ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatc
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacccactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaattgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag cacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 cttgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaaagaggct gggacagaag
    12961 cgtttaactc caagaaataa gaaggagttg cttctgcttc cgccgtttat acgtgtttag
    13021 agcgtgagac aaaagtgttc tttacttttg tctcacgctc tttttttgtt atattcgtct
    13081 tttccgatcc ttctcttgac acaattcatt taactgatca ctggtacttg aacacctaat
    13141 gttctagtca tttttataaa cgattcaata cttcatccac atttttgatc atcacggata
    13201 tcgtaccatc cggattattc acaaattcaa tcaaatctgg gttgcgatat acatctactg
    13261 gtacgatcag ttcgatcccg tttgatagct tgaatttttg ttttccaaat tttttctcag
    13321 aaatttcttg tacttctttt acaggaggtg cttctgggac aaatccttgc tcttgtactt
    13381 cttctttaaa agccattttt gccgtgatgt tttccttgaa gactttttcc gcaatcatct
    13441 catgatccaa ccgacctttt tcttctatcg tatcatagac agcttctttc aaatcagcca
    13501 tgatatcaaa ttcttctgtc tcaaattttt ttccaagatg cttggctact ttttggatca
    13561 cttttacatt ttcttctaaa gaaggtgcag gaacactttc gatcactctt ccagaaaaat
    13621 aaagctcttt tttgccagaa aactcatatt ttttttcaat taattcgtag gtccaatcag
    13681 tcaaattcac agcgaatgct tcatctgctt tttgtgtttt cgcccctaga attgctcgat
    13741 tcaaaatcaa gcggttgtcg atccctgctt cgtctgcttc aacaaagtga gtgtaagcct
    13801 cacgataatt gactttcaat aaagataagt acatcactgt atctaattca tacaaaacaa
    13861 caaatacatc tgcactaggt gcttcttcac tttcttggta gatatcgaac caccgctgaa
    13921 cgattgcttc acttgcttcc acaaaatttt cttgagcgat tttaagtgat tcaacgatca
    13981 tgctttcttc tgctagttgt ccagtttttg tttgagcaga agagattttt tggatcttct
    14041 tttgaagaaa atcacgaata tattcttttg tcagatccag ctctttttgt gaaaagactg
    14101 gagaaccagt ttctctgtca ataatatgca gaattgcttt ttttaaatag atatccattt
    14161 tctcattccc ttctgtctta gtttacggaa aaaaaatcaa aaataccaga atcaaaatca
    14221 ttttctttta aacaaaaatt tcaagagtag aaacaaaatg gattcttttt ttcgaaaaat
    14281 gcgctacact tatagttgaa cgaataaaaa aggagctgaa agtatgaatt cattaacaag
    14341 cacttatcgt ctattaaatg gttacgaaat cccggtagta ggattcggca catggcaaac
    14401 acctgatggg gatgtcgctg tttcctctgt taaagaagcg ctggctgcag gttatcgcca
    14461 tatcgatact gcccaaggat acaagaatga agaaagtgtc ggccaagcga ttaaggaaag
    14521 tggaattcct agagaagaga tattcttgac aacaaaatta tggaatgcaa accacagcta
    14581 tgaacttgtg atgtcttctt ttgaagaatc attgaagaag ctccaaacag attatttaga
    14641 tctgtttttg atccactggc caaaccctgt tgcttttcgt gataattggg aacaagcaaa
    14701 tgcagataca tggcgagcat ttgaagaact gtatgaagca ggtaaaatca aagcaatcgg
    14761 tgtgagcaat ttcttacccc accatctaga tacactagca aaaacagcaa aaattatgcc
    14821 gatggtcaat caagttttcc ttgcaccagg cgaattacag ccaactgtag ttgagtatgc
    14881 aaaaaaacat gatatgatcc tcgaagcata tagtccatta ggcactggta aaatcttcga
    14941 tgtgcctgaa atgaaacaaa tcgctgaagc acatgacaag acgattgcac aagttgcttt
    15001 gcgttggtcg ctgcaacacg aattccttcc tcttcctaag tcagtgacac caagcagaat
    15061 caaagaaaat actgaattat ttgattttga attaacagaa gaagaaatga aacaaatcga
    15121 tcagttagat ggtgtagtcg gcaaagcaaa agacccagat actacccaat tttaagtcga
    15181 aaggtgattt tatggatcca atcgaacgct tgaattctct ctcggaagaa gttacccaga
    15241 cttttcattc cgattttgtc tttttgatcg atgcagagaa aatccaacat tttcctgcaa
    15301 gaaactggac acatgatcaa atcatcgaag aactgaaaaa acggttcgat cattcgctaa
    15361 tggtcaaacc ctggcacgaa catgaagtca tttattcacc tgaactacct gtttttgcct
    15421 tgataccgaa aaaatagaaa gaatggaacg gattatgtat atatacataa tccgttccat
    15481 tttttatttt tctttcaaaa aacgggaaaa tacgcaaatt tttctgttgt aatttgcgta
    15541 tacgcaagtt tttttgcatt actccttttc atttggctgt ttttttctta taatcgaaag
    15601 ggtatgagcg aaggagagaa catatgaaga aaaaacagaa acaaaaatta ttaaatcaat
    15661 tccgtccttc attggatggt gtaagaggac aattattccg ttctctagaa gaagagagct
    15721 tgcgacgcta tggtttgccg ttacaagtca tgcttgatcc caaaaaccgt caaatcttaa
    15781 cgatcggatt agctgggaaa acaaatgaag atacacggat caacgtgcca atcgatgaca
    15841 acttcaatac tgtattaaag agaattcgtt caggagaaaa cgggattttc gaacgattcc
    15901 gtgacaatct tcttattgaa atcgtgtctt actggaatga tcaacgattg aaaaacgatg
    15961 aaccaactgc gcaagctgtt gcgacaccag ttacagaaga aacagtcagt gtgccagaag
    16021 aaaaaacgga aaaaccaaaa gcggacacaa ctgaaaatac acgctcaact gatacgaatg
    16081 ccgctttaag ttttgctgat ttttcaaaag ccgttgctga gtatcctaaa ttttacacag
    16141 aacaaaatga acatgaagta gttattaatg aaaaaaatgc tgatgaacct cgtaaattag
    16201 caactatttc tatgactact gaaaatgagt tcgttatcga aaaagcactc gaacggaaat
    16261 acaaagtcaa attgactttg attcctttga tcgaacaatt tgcagcgaca gcgattactg
    16321 atcgataaaa attctatttg tcataaaagg ctgggattta aaatcccagc ctttttagtc
    16381 atataataat ttctacagca agtaaaaaga aattccttct aacatcaggc tcaaatatca
    16441 gcttgacctg tccaacaaat cacctatgat cctacaatgt taggcgactt ctcttctacc
    16501 tgaagatatc ttggagttgc ctgatttctt aagatgccta accttaacta taaaaaattc
    16561 taataataaa catattttct gatagagaaa aaagcaagat ttgcagtaaa taaaataact
    16621 gtaaatcttg ctttattctt ctaaagatat tgctttttta tttttcccgg attttttcca
    16681 atatttgtat agctgtctgc tggttcatct tttctgcttc acgtaatttt ttcgcaactt
    16741 gctcgatttc ctcaccaata gccccaattg agatcgctaa tgaacgagct tgaagtccca
    16801 tgtggccttt ttggattcct tctgtaacta atgcacgcaa agcagccagg ttttgagcta
    16861 accctactgc agcaattact tgtgctagtt ccttggctga atcaatatct aacatagcta
    16921 aagatgcttt tgcttttggc aggatattgg aagctccgcc tactgtggca acagccaaag
    16981 gcaccgttag ttttccaact agtttatccc ctttgatctg ccagtctgtt aatccttggt
    17041 ataatccatt tcttgccgcg taggcgtgaa tcgaagcgct aaccgcacgt gtatcatttc
    17101 ctgtagctgc cactacagct tctataccat tcatgatccc tttgttgtgc gttgctgcac
    17161 ggtaagggtc aagcttcgca tattcccctg cctgttggat ttttttggca atttgttcac
    17221 cgatttcttt gtttctccct aagcgttcga atgggatttc acaacaggca gaagccaatg
    17281 attctgtagc aaaattactt aaaatactga atagaatctc ttcttcaggg aaccactccc
    17341 gtaacttgtt tgccacactc tcaagaatcg agttgatcat gttagcgccc attgcatctt
    17401 ttacatccac taaaaaatca atagataagt atgcatggaa actccccatg aattcccgag
    17461 tagagatatc ttgaacaccg cccccgcgtt tcacgattga aggataactt tcatttgcac
    17521 aaagaatcag ctcttctttt cgatgattaa ctgcattgat cactgcttga tactccgatt
    17581 ttcctgatag aacgatttgc cctcgcatca gccgctgagg tgtttctgcg cagatatttc
    17641 cacagatttt tgcaccatta cttgcagctg caatgacaga tggttcctct gttgccatcg
    17701 gaatccattt tttcttacca ttgatttgaa aattctgcgc gatgcccatt gggatttcca
    17761 cttcactcac ttggttttcg atcatgtgat cggagagctc ttcagaaagt gtttgttcct
    17821 gaaaaatcaa cgcagtttct tgtgtcaata catttttttc tattaattga ctgcgccgtt
    17881 cagatggcgt aagctgataa aacttttttt ttgcacatcc ttatgtgttt gctccatatt
    17941 cgcttctaac aagactgcca agcctaaacc tccaccaata cataatgaag caataccgta
    18001 tctcttctgt tcacgaagca atccgtaagc taatgtagtc aagatacgtg caccgcttgc
    18061 accaattgga tggcctaatg cgattgctcc tccatatata ttgacctttt cttcatctaa
    18121 ctgcagttct tggctaacta cgatagaaga agctgcaaat gcttcattga tctcaaaaag
    18181 atcgattgtt gataaattca ttcctgatct atctgtcaat ttttgaatgg ctttgattgg
    18241 agcgattccc atgatacttg ggtcaatacc gacctccgct acttctttga tggttgctaa
    18301 ataaggcaga ttattatttt ctgcgtattc tttggatgca aggatcacca cagaggcacc
    18361 gtcattcaat ggcgaagcat ttcctgctgt tacagttcct tcttctgaga atactgtacg
    18421 taacgtgccc agtttttcca atgtactatt tccacgaact gcttcatcct cagataacac
    18481 gtcttcatca gaaatcttga ctgggatgat ctcctcagaa aatacaccgg cttcgacagc
    18541 atgtgctgct ttcaactggg acgacaaggc atagcgatcc tgttcttctc tgctcacaga
    18601 aaattgtgtt gcaaccttct ctgcggttaa tcccatatgt gcatttgaaa atgcgtcagt
    18661 caatccgtcg ttgaccatac tggaaattgg ttcaccatat tcatttgttt ctgactgata
    18721 cggtttcagc ataggtgctt gagacatact ctctgttccg ccggctatca caagttctgc
    18781 ttcacctaat tggatcagct ggcgtgctaa gatcactgct ttcataccag atccgcaaac
    18841 ttcattgatc gtcattgctg ggacatctac agggagaccg ctgttcaaag caatctgtct
    18901 ggcaacattt tgaccattac cagcttgcag gacatttccg aatattactt gatcgatttt
    18961 atcttttttc agttttgttt tatctaataa tcctttagtc acaagtgtgc ctaattcaac
    19021 tgcagtaaat ggacttaaac ttccgcgata ctttcctatc ggcgttcttg cagcatctat
    19081 cataacgact tctttcaagt aagccacctc ctacaaaaat ataccattct ttgctgtaaa
    19141 aacaaatagc tcttgttcca gttgtttttt tctaagatta aattttaaca atccttaaat
    19201 attctctttt ttatatttct tttaatcttt ttatgatacc ttagtaaagt aagtttttta
    19261 tcataagtaa agaggtgttg aaatgaaaat agggattgat cgtctttcct tttttattcc
    19321 taatttatat ttagatatga cagagttggc agaaagccgt ggggatgatc ctgcaaaata
    19381 ccatataggg attggtcaag accaaatggc agtcaatcgt gcaaatgaag acatcattac
    19441 actaggagca aatgctgcca gcaaaatcgt aacagaaaaa gaccgtgagc taatcgacat
    19501 ggtcatcgtc ggaacagaat ccgggattga tcattcaaaa gcgagtgcgg taattatcca
    19561 ccatttactg aaaatccaat cttttgctcg ttcttttgaa gtaaaagagg cttgctacgg
    19621 tggcaccgca gctttgcaca tggcgaaaga atatgtcaaa aatcatccag aacgaaaagt
    19681 actagtcata gcaagtgata ttgctcgtta cggcttggca agcggtggtg aagtgacgca
    19741 aggtgtcggt gctgttgcga tgatgatcac tcaaaacccg cgtattttat cgattgaaga
    19801 cgacagcgta tttctgacag aagatatcta tgatttctgg cgtccagatt atagcgaatt
    19861 tcctgttgtt gatggtcctt tatctaattc tacgtatatc gaatcattcc aaaaagtttg
    19921 gaatcgacat aaagaattgt cgggtcgagg actcgaagat tatcaggcga ttgctttcca
    19981 tattccgtat actaagatgg gaaaaaaggc attgcaaagc gtattagacc aaacagacga
    20041 agaaaatcag gaacgtctta tggctcgcta tgaagaaagc atccgttaca gccgacgaat
    20101 cggtaatctt tacactggtt cattatacct ggggctaact tctctactgg aaaattcgaa
    20161 atcactacag ccaggagatc gcatcggtct attcagctac ggttctggtg cagtaagtga
    20221 attttttaca ggttatctgg aagaaaacta tcaagaatat ctcttcgcac aatctcatca
    20281 agagatgcta gattctcgaa cacggatcac tgttgacgaa tatgaaacga ttttcagtga
    20341 gacgcttcct gaacacggag aatgtgcaga atatacttca gatgtacctt tttcgattac
    20401 aaagatcgaa aatgatattc gttactacaa aatataataa aatcataaaa agaacccttc
    20461 acgagtcgtg aaaggttctt tttttatctg cggaccaaag gagcagtcta taggtcagct
    20521 tagtcgtact aaggatttct cgtttttcac tgaatagttt gcgaatgtac ctggtcacaa
    20581 caggatcttt tgcgtacgcg tcatagtata gcttcatcaa ttcttcagta attgattcct
    20641 gtgtaaaata agtaaaatct tttttcttca ctttgaatcc ttcatcaatt aagtatgttt
    20701 caaattgatc cattacaact ggcgatgtat taggatcttc ttctatcatt cctagttgct
    20761 tttcgatagg ataaaaaaca tcatcgattg attccactac cctgattatc acgcaccatt
    20821 tgcttgactt tttcatcaat ttttctaatt gaataagatg taccaatgct ggatttgctg
    20881 ctgtaaaaac catttcatat gtctcatttg attggataaa ttcttctaac gactgtttga
    20941 taaaggaaac atttggcaac tcattttttt ctgcttcttt ttttgcattt tctagcattc
    21001 ctgaagaaaa atcgatcaaa tcatagcgat taatttcttt tgctaaggaa agaaatctgc
    21061 cagatcctcc agcaacgtct gctgctgttt cagcaggcag gactttctcc gttttcagat
    21121 aattttttac atcttcagca atagttactt gcgattcttg ctggatttca taatattctt
    21181 cagcaaattc atcccattct ttttcattca tttgatttcc tctaattttt tgttatatgt
    21241 tttcactttt cggaataaat tattctcctc ttttatctat tctatctctt ttgatgaata
    21301 ataaaatgat tggctttccg tctatcataa ataaaaaaag ggactgtgac caaagtcgtg
    21361 tcaaagtccc tctttccgaa taaacagtgt ccaatcagat gaccctcagt attagctcaa
    21421 aatgctgatt ttacaacttc ttccttaaac ggtgttctcc tgccaactct tcggtcacaa
    21481 gtcacaatat tcaacaaaca tgagaagctg tttgttgaaa ttgcaccttg tgactcagaa
    21541 ttaaacggca ggctcacaac ctcgttgatt cgttttattt atttccttgt actcgtacaa
    21601 tgatcaattt agtgatttcc atcaaaacaa ttgtcacaag tgcgattccc atacatgtaa
    21661 agacttctgt caatccaaat gctgatggga tagagaagac ttctttcagt cctgggatca
    21721 ttgttaagca gtaaagcgcg ctacatacaa ctacagctag taagacactt ctgttagaga
    21781 agaaccctgc ttggatagct gtttgtgtgt ttgaacgtgc tgggaatgtt tgcaatgtcc
    21841 gacaccaaat caatgttgaa aatgacatgg ctacaccgaa ttcaggtgaa tgctgcatgc
    21901 caagccattg agcaaagata acagcaatcc caatcagcac accacgatac gttacagaaa
    21961 tcaatgtttt gtccgtaaag attcctgaat ttggatctct tggcgggcgg ttcatcgtag
    22021 caggttcagc tttttctgta cctaatgcaa ttgctggcag tgaatcattg actaaattga
    22081 tgaacaacag ttgtaatgct gtaaatggat tggaccagtc agcaactaag gcaaagatga
    22141 tcgcaatgat cgcacctaaa tttcctgcaa atagataaga aatagctttc ttgatattat
    22201 caaacacatt acgtcctact tctactgcgc tgacaataga agcaaagtta tcatccgtca
    22261 ataccatcgc tgctgcatct tttgccacat ctgtacccgt tcccatagca attccgatgt
    22321 ctgcttgttt caatgctggt gcgtcattta caccatcacc tgtcatagct gagatatgac
    22381 ctttattttg ccaagcacga acgatacgaa ttttattctc aggtgaaaca cgtgcataaa
    22441 cagtgacttt ttctaaaact tcgtttagtt gatcttctgt caattcatct agttccgtcc
    22501 cggtcaaagc caggtttcct tcttcaaaaa tcccgatttg ttttgcgata gcaacagctg
    22561 ttgttttgtg gtcaccagta atcatgattg ttttgattcc tgcacttctt gcttctgtta
    22621 ccgcctgagt cacttcagca cgtggaggat cgatcatagc aagcaaacca acaaggatca
    22681 aatcgtcttc atcttcaaat gataaatcaa aattatcaag tggacggtaa gcaaaagcta
    22741 gtacacgtaa tgcgcgtgtt gaaaaagctt cattttgctc ttggaatttt ttcatcaatt
    22801 catccgtcat cggaacgact tcaccattta gcaatacttt tttactgcgt tggaagacga
    22861 tatctggtcc gcctttgaca aacaagtaat tttcgttatc gatcgtgtgt ccagtagaca
    22921 ttaattttcg atcagaatcg aaaggaagtt ctgcttgtct tgggtagttg tttcggattt
    22981 cctgataagg ctggtttaat ttttcactaa agtcgataaa cgctacttcc gtagggtcgc
    23041 ccaatttagt accgtcttca ctgatcgatg catcattcgc taaaacactg atttcgatca
    23101 agcgacgttc gtcagctgtc cattttgaag ggtcatttgt gaaatcccca gtatcgccat
    23161 tacctaaaaa gtagtcaaca actgtcattt tgttttgtgt caatgtacca gtcttatctg
    23221 tacagatgat acttgtcgaa ccaagtgttt caactgctgg aagcttccga ataatggcat
    23281 gacgttttgc cattttattc gttcccaaag ataacacgat tgttacgata gactgtaatg
    23341 cttctggaat agcagctacg gctacggcta cggcaaacat cactgcgctg acgatatctg
    23401 cagtcatatc tcctgtacct tctccaagat aaatacgaaa cagttggata cctaaaatca
    23461 ataaagacaa tgccaaaata gccagactta gttttttact gaatttatcc agacctcgtt
    23521 gtaacggagt gacttgctca gttgttgatt ccaaaaggcc agcaacttgt ccgatctccg
    23581 tattattccc agttgaagtc actaagaatt taccgcgtcc gtaagttacg atcgttccac
    23641 tgaataccat atttgtacga tctccgattg gtaccgtact tgtgatatcc gttacttctt
    23701 tttctgcaga agtcgactct cctgtcagca taccttcatc tacttttaat gatcccgcat
    23761 ctagtaaacg tccatcagca ggtacataat cgcctgcgtc taacaacacg atatctccta
    23821 ccacaagttc gtttgccgga atcgtttttt ccttaccatc acgaatcact cgagcatcgg
    23881 gcgctgaaag gtttttcaac gcttctaaag aaccttctgc tttttttgtt tggacaacac
    23941 tgattataga attcaacatc aatactgcaa aaataactaa gaattcgatg tagtcaccca
    24001 tgaccatctg aatcagtgcg acaatcagca atacgataac cattgcatct ttgaaagttt
    24061 ctaagaacaa tttccaagtt ggcttcttcg gcttttcttt tagctggttc aagccatctc
    24121 tttctaattt ttccttcgct tgcgtggtgc tcaatccatc ggttgagctc tgcgtttgct
    24181 gcatcaacga gtcgatcgtt tctttataga ccgcttgttg tgcttgttcc ttgttttcca
    24241 aatttcatcc cccatttttt gatcttggcc ctagtcctgt ctgaaaaaaa aagagaccta
    24301 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
    24361 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
    24421 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
    24481 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
    24541 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
    24601 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
    24661 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
    24721 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
    24781 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
    24841 gatcctgaca tgggcgataa agacaaacag caatttgtcg acttcttctc ttcattaaat
    24901 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
    24961 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
    25021 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
    25081 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggtacaaa agcaaaagat
    25141 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaaaggca
    25201 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
    25261 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
    25321 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
    25381 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
    25441 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
    25501 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
    25561 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
    25621 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
    25681 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
    25741 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
    25801 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
    25861 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
    25921 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
    25981 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
    26041 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
    26101 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
    26161 caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag
    26221 gctcacgacc tctttctttt actctaattc accagctgcc caatgattga tgggtccata
    26281 tttgtgccct acatttattt cttcacgaat tgcacggttt acatagtctt ttgccaatcg
    26341 gatcgcttgt tcaactgttt ttcctttcgc taactcagct gcaatacatg cagatagcga
    26401 atcgccagtt ccgttgatcc ggtcggtagg ataaaatggt gcatttaacc aaaatgattt
    26461 tcctgattcg agcaatacgt agtcattgac tgtttcttgc tgcgcatctt tagcatgatg
    26521 tcctttgatc ataacgtttt ttgcacccat atcttgcagt tcttttgctg cttgcacaaa
    26581 atcttcttca gctgctaatg tccgtccagt cagcttttct gcttcataaa aattaggtgt
    26641 cagtacttcg gccaaaggga caagcttttc tttcaagctt tgcaaagcag attcttctag
    26701 taaaagattt ccatgtttcg ttacgattac gggatccacg actaatggtc caaaatcata
    26761 tttttggtaa ttttttacaa ctgtctcaat caactttgaa tctgctagca tacctgtttt
    26821 agctgcacgt atttgaaagt ccttcgcaat cagttcaaat tctttgtcga taaaatctgt
    26881 tggcaatgtc acacttgctc cgattccgta agaattacct gcaacacatg cggtcatgac
    26941 agaaacaccg taaacatttc ttgtaaagaa tgtatgcata tctgcttgca tccctgcgct
    27001 tccatcgcta tctgatcctg cgatcgtcat tgcttgtaca tactgatctt ccatcaaatc
    27061 gattcctcca tttttataaa aagatttttc ataatgctgt tactgtttct atcatatgtt
    27121 tttcaaagta tccctcagta aatataccac taccttttct tggtaatcaa atttatttga
    27181 aaaaacattt gtaataaatc agaaaatagt tgtcatttgt ttgtattcgc tattctaata
    27241 ataaataacc actatttgat ttatatatat ttcagtatcc ttcttgtttt catataacca
    27301 aaggcgctat gatttccata tgaaaaaaat aaagtcaggt gaaaaaaatg aaacttgagg
    27361 ataaaaaagt cattttgaag aaaatcaaac gactaccaga ccaaatagat ggctatacga
    27421 tctgttcttt caaaaaagac aggagtataa aagcttatac gcaaaacaca gaaaaaggaa
    27481 taatctattt cctacatgaa gaaggttacg atacccaaac ctttatgttt actgaaaaaa
    27541 aagaatttca taaacagatg aaaaaactga ttgaaaaaga atttccaaga agtcataaac
    27601 tctatgtgaa tcagcaattt taacttctaa ctgttcttta
//
LOCUS       DACDVT010000031        26150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDVT010000031 DACDVT010000000
VERSION     DACDVT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.031"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            227..1231
                     /locus_tag="IWL52_001380"
     CDS             227..1231
                     /locus_tag="IWL52_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777952.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1232..2176
                     /locus_tag="IWL52_001381"
     CDS             1232..2176
                     /locus_tag="IWL52_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9777953.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2176..3138
                     /locus_tag="IWL52_001382"
     CDS             2176..3138
                     /locus_tag="IWL52_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777954.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3165..4079
                     /locus_tag="IWL52_001383"
     CDS             3165..4079
                     /locus_tag="IWL52_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9777955.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4105..5886
                     /locus_tag="IWL52_001384"
     CDS             4105..5886
                     /locus_tag="IWL52_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9777956.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNDAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6234..6920
                     /locus_tag="IWL52_001385"
     CDS             6234..6920
                     /locus_tag="IWL52_001385"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9777957.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6940..10521
                     /gene="smc"
                     /locus_tag="IWL52_001386"
     CDS             6940..10521
                     /gene="smc"
                     /locus_tag="IWL52_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9777958.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEVLAKEQPKMYQLMNQVQQLRARQKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIHYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCSVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10530..11339
                     /locus_tag="IWL52_001387"
     CDS             10530..11339
                     /locus_tag="IWL52_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9777959.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11351..12349
                     /gene="ftsY"
                     /locus_tag="IWL52_001388"
     CDS             11351..12349
                     /gene="ftsY"
                     /locus_tag="IWL52_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9777960.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12536..14803
                     /gene="gshAB"
                     /locus_tag="IWL52_001389"
     CDS             12536..14803
                     /gene="gshAB"
                     /locus_tag="IWL52_001389"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9777961.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTGRNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFADQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14892..15344)
                     /locus_tag="IWL52_001390"
     CDS             complement(14892..15344)
                     /locus_tag="IWL52_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9777962.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15494..16444
                     /locus_tag="IWL52_001391"
     CDS             15494..16444
                     /locus_tag="IWL52_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9777963.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLAT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16437..17396
                     /locus_tag="IWL52_001392"
     CDS             16437..17396
                     /locus_tag="IWL52_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9777964.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     GFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17393..18148
                     /locus_tag="IWL52_001393"
     CDS             17393..18148
                     /locus_tag="IWL52_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9777965.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18171..19124
                     /locus_tag="IWL52_001394"
     CDS             18171..19124
                     /locus_tag="IWL52_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9777966.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPNLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19647..19943)
                     /locus_tag="IWL52_001395"
     CDS             complement(19647..19943)
                     /locus_tag="IWL52_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9777967.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20298..20654
                     /locus_tag="IWL52_001396"
     CDS             20298..20654
                     /locus_tag="IWL52_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9777968.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20664..21563
                     /locus_tag="IWL52_001397"
     CDS             20664..21563
                     /locus_tag="IWL52_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9777969.1"
                     /translation="MGNLAIISDLHVDINKFGEPELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFANKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22080..22811
                     /locus_tag="IWL52_001398"
     CDS             22080..22811
                     /locus_tag="IWL52_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9777970.1"
                     /translation="MMKKEIVLTAATMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGPGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYMPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22956..24983
                     /locus_tag="IWL52_001399"
     CDS             22956..24983
                     /locus_tag="IWL52_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9777971.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISVVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDVEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25091..26150
                     /locus_tag="IWL52_001400"
                     /pseudo
     CDS             25091..26150
                     /locus_tag="IWL52_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
BASE COUNT         8708 a         4560 c         5557 g         7325 t
ORIGIN      
        1 tttttatcct tcttttttta atctttttga cacgtaaaag tcatttttct gataattctg
       61 gcgataaaca gaaattatgt tgcaaagtaa agcggttttc tatataatta ttaataattc
      121 attacacaaa gataagaata acctttgtta taaaacgatt tattctgtgt aatgtccttt
      181 tttattaaga aaaacttcca tttaggaagc agagaggggt attcgattgt ccgataacca
      241 attattagaa gttcagaacc tacatacagg attccgactc aaagatgact attacgatgc
      301 agtagatgac gtttcattta ctttagacaa aaacgagatt ttagcaatcg ttggggaatc
      361 tgggtgcggg aaaagtacct tggctactac gattatggga ttacttgatc ctaataacac
      421 aaaaattaca ggtgagattt tatataacga tctgaattta atcgacctga acgaaacgct
      481 ctataataaa atccgcggaa atgatatcgg aatgattttc caagatccgt tgtctgcatt
      541 aaatcctctc atgagaatcg aagaccagat caaagaagga ctttcctatc acacaaagat
      601 gaatgcagaa cagcgtcaag ctcgtgcact agaattgcta gaacaagtag ggattcctaa
      661 tcctgcgaga gttggacgcc aatatcccca tgaactatca ggcggtatga gacaacgtgt
      721 catcatcgca atcgcaatcg cctgcaaacc tccaatcgtc attgcagatg aaccgacgac
      781 agccctagat gtaaccatcc aagcacagat tcttgattta ctgaaagacc ttcaggaaga
      841 aacaaaaaca gggatcatct tgatcactca tgacttagga gtcgtagcag aaatggctga
      901 tcgagtggca gtgatgtatg gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt
      961 tgatcatcct aagcatcctt atacacgctc tttactgaac tctataccac aagaaggaaa
     1021 tcatgatgca gaactccatg tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg
     1081 taaaggttgt cggtttgcac cacgtattcc atggatacca gcaagcgatc atgaagaaga
     1141 gcctatcctt catgaagtcg caccgaacca cttggtacgt tgctcctgct ataagcattt
     1201 ccactttaga gacgaaaaag gagaggtgta gatgacagaa ttaatcacaa tcaaagacct
     1261 gaaagttcat taccctattc gcagtggatt cttcaatcga gtaacggatc atgtatatgc
     1321 tgtagatggt gtggatttta ttatcgaaaa aggaaagaca tatggtttag tgggagagtc
     1381 tggctctgga aaatctacta cgggaaaagc cgttgtggga ttagaaaagg taacagccgg
     1441 acaaatcatg tatgaaggaa aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta
     1501 taacaaggat gtgcagatga tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg
     1561 agtgttggat atcattgctg aacccatccg aaattttgaa cggttaagcg atcaagaaga
     1621 aaagaaaaag gtaaaaggac tgctagatat cgttgggatg ccggaagatg cgctatataa
     1681 atatcctcat gaattttcag gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc
     1741 taccaatcca aaactgatcg tagccgatga acctgtatct gctctggatc tatctgttca
     1801 agcgcaagta ttgaatttca tgaaacgaat ccagcaagaa tacggcctca gctatttatt
     1861 catttcacat gatttaggtg tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa
     1921 aggtcgattt gtagaaatcg gtacaagaga agacatttat aatgacccgc gacatatcta
     1981 tacgaaacgt ctgctttcag ctattccgca aatcgatgta gaaaataggg aaagccacaa
     2041 agaaaatcga cgaagagtag aacaagagta tatccaaaac caaaaagaat actatgatgc
     2101 gactggtcga gtctacgatt tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga
     2161 tggaggtgca agctaatgtg gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa
     2221 gttattattt taagtgttct gatcttctta ttagcccaag cgatgccggg ggatccattt
     2281 accggtttga tcaatccaaa tcaggatccg gcagtgatcg aacagatgcg acaagcagct
     2341 ggcttgaatg acccatggta tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc
     2401 gattttggtc aaagtttctt atacaaacgc tctgttgctg acttgatcgg cgaacggatc
     2461 atcaacactt tatatctatc gatcttgaca gttgtcctta cttatttgat tgctgtgcct
     2521 ctggggatgc ttgctggccg gtatcaaaat tcaatcttgg ataagattgt cgtaatctac
     2581 aacttcttta gttttgctgt tcctttattt atcttcggct tgatcatgct atttgttttt
     2641 ggttatcgtt taggctggtt cccaacaagt ggatcacaaa cactaggttt gcaaggtgga
     2701 tttatcactc aatggataga ccgtttgaaa tatattctgt taccatcaat tacccaagca
     2761 tttttggcga ctgccgtcac aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg
     2821 ctcgattttg tcagaacagc ccgttccaaa ggggtaccga cgaataaagt attcagtcga
     2881 catattttcc gaaatgcagc gttgccgatg gcttctcaaa tggggtatga aatcacttcg
     2941 ttgattgctg gttctgtagt tatcgaaaag atttttggtt atccgggagt cggaaaacta
     3001 tttattgatt ccatcggcca acgggactat accgtgatca catccttagt attgatttta
     3061 ggaatcgcga cgctagtggg gacattactt tcagatatca tcatgagtat cgttgacccg
     3121 cgtattcgga ttcaataaaa ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca
     3181 atacagcagt tcaagaaagt attcctccta tggggatacg aatgattgct cgagaattta
     3241 aaaaagataa aattgctatt ttttcattag gtttgctagt ggttttatta ttagtgatct
     3301 ttatcggtgc gatgctgacc gatcaagaca aagtgatgta tgttagtatc ttcgacaaat
     3361 acgctgcacc aggaacagtt tcgacacaag gacaaaagtt cattctggga gcagatgaag
     3421 gcggacgtga tgtattagga caattgatca taggtgctag aaactctgtt acgatcggat
     3481 ttgcgatcac gatccttact tcgatcatcg gggttggatt agggatcatc tctggctact
     3541 acggagggat cattgacaac atcttgatgc ggatcgttga ttttatcatg attctaccga
     3601 tcatgctgat cattatcgtc ttcgtatcga ttatttccag atacaatgtc tggtccttta
     3661 tattcatcat gagtgcattt tattgggtag ctaaagcccg attattccga agcaagacat
     3721 tatctgaagc aagaagagat tatgtcagtg cctcaaaaac aatgggaacc aatgatttta
     3781 aaatcatgtt ccgcgaaatc atgccgaatc taagttcatt aatcattact aatttgacga
     3841 tgaactttgc ggcaaacatc ggtatcgaga caactttgac tttcttaggt ttcggcttgc
     3901 cggcaaatgt acctagttta ggtacattga tcggttatgc aagtaacgga gaagtcttag
     3961 tgaatcggca atggatctgg ttacccgcat caatccttat tttagttttg atgttgagta
     4021 tcaattatgt tggacaagca ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt
     4081 atagaagcga agggggaaat aggaatgaag aaaacgattt ttggatttgc agcgctgatg
     4141 tctgctgtcg ttcttgcagc atgcgggggc ggaggtacag atacctctgg aaacaactct
     4201 ggaggcaacg gcaataacaa agaggaatta acttttccaa ttgcaacaaa aaataatgat
     4261 gatgcaatcg aagacggcga attagaagct gcggtcgcaa cggacagtca gtttaaagga
     4321 ttgttccagt gggagtttta ccaagattca tatgatgcag cttttatggc tccttctcat
     4381 gaaccgctat ttgcaaacga tgacgatttc agaatcacag acgacggagc tgcaacgctt
     4441 gatctagatg aagatacgaa aaaagcgaca atcaaagtaa aagacaacgt caaatggtca
     4501 gatggtgaag atgtaacggc ggacgatgtg attttccctt acgaaatcat tgggaataaa
     4561 gactacactg gtatccgtta tgacgacact ttccgtaaca tcgtaggtat ggaagaatat
     4621 catgatggaa gtgccgacac aatctccgga atcaaaaaaa tagacgacaa aacagttgaa
     4681 atcgaatata aagaagtaaa cccaagcatg cttcaatcgg gtggtggtat ctggtcgtat
     4741 gcagcaccaa agcatacgtt agaaggtgta gcaatcaaag atatggaatc aagcgatcaa
     4801 gtacgtaaaa atccagtgac ttttggccct tattacatga gtaatatcgt tgcaggagaa
     4861 tcagtagaat acttgccaaa cgagcactac tggaatggta cgcctaaatt gaaaaagatc
     4921 acgatgaaat ctgtaccgat ggcaagtgca actgaagcat tgaactctaa attatatgat
     4981 atgattttcc aaatgccaac agatacttac gatacctata aagaaattgc tggctataca
     5041 aacttaggcc gtgaacaaac ttcttatact tatctaggat tcaagttagg taaatggaat
     5101 gcagatacaa agagtgtgga atacaatcca gattcaaaaa tggctgataa atcattacgt
     5161 caagcaatgg gctatgcact agataatgat gcagtaggtg aacgtttcta tgcaggtttg
     5221 agaagcaatg cgacttcatt gattcctcca gtattccagt cataccatga tgatgagatg
     5281 aaaggattca cacaagatct agacaaagcg aaaaaactat tagatgatgc tggctacaaa
     5341 gatgtggatg acgatggttt aagagaagac aaagatggaa ataaactaac aatcaacttt
     5401 gcttcgatgt ccggtgggga aacagcgcaa ccattggcag attactatat ccaacaatgg
     5461 aaagaaattg gtttggatgt ccaatacaca acaggtcgtc tgatcgaatt caattctttc
     5521 tatgataaat tagaaaacga tgatcctgaa atcgatgttt accaagctgc ttggaataca
     5581 ggaactgatc caaatccaag tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt
     5641 tatgaatcag aagaaaatac aaaattgatc aacgatatca gttcaagcaa agcatttgat
     5701 gaagagtacc aaaaagaagc atatcaaaaa tggcaacaat atgcgtttga agaagcattt
     5761 gcgattccaa cgttgttccg taatgaagta ttgccagtca acgaccgtgt caaagactgg
     5821 gattggtctt atgatgtacc aaacccatgg gcaaatgttt ctgttacagc agattcacgt
     5881 tcataatcac taaaaatgac cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt
     5941 tctgtttatt tctctaatat tcaggtcaag gcgctgtttc catgacttct ccaataaacg
     6001 gtgttcaaag tccagtttct cggtattaag tcaaatttat gcaaaaacat ggaaagtcat
     6061 ttttacaaaa ttttttcttc atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa
     6121 tcactaggat tattaaagct ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa
     6181 aaccagtata ataaggaagt atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca
     6241 accagctgac aaaagaacta aaagaaaact ataacatcgt ttttcacgat ctaaatttac
     6301 ttgaacaagc ttttacccat tcatcctatg tgaatgagca tcgcaatctg caattatcgg
     6361 ataatgagcg acttgaattt ttaggagatg ccgttttaga actgatggtt tctcagtatt
     6421 tattccgttt atatcctcat ttgccagaag gaaaattgac aaaaacacga gctgcgatcg
     6481 ttcgcgaaga cagcttgtcg aaatttgcca aagaatgtca ctttgatcaa tatgttctgt
     6541 tgggcaaggg agaagagaat tcaggaggac gtacgcgacc agcattgctt tgcgacctat
     6601 tcgaagcttt tttaggcgcg ttatatcttg accagggatt cgacacgacc catgcattca
     6661 tcgaaaaagt gatttttcct aaagtcaaag caggtgcttt ttcacatgag atggatcata
     6721 aaacaaaact acaagaagtc ttgcaaaaat caggtgatgt atcaatcgaa taccgattga
     6781 tcaatgaaga aggtccagct catgaacgag tattttggat cgaagtttat gtagatgatc
     6841 aattgattgg cactggacaa ggaaaatcga aaaaattagc agaacaagct gcggcggaaa
     6901 atgcattggc agcactgtaa aagtaagtga gggaaagctt tgtatttgaa acgaattgaa
     6961 atagccggtt ttaaatcatt tgcagacaag acagtcattg attttgaaaa tagcgtcaca
     7021 gcagtggttg gaccaaatgg cagtggaaaa agcaatatta cagaagctat ccgctgggta
     7081 ttaggagaac aatctgctaa aagtctgcgg ggcggaaaaa tgccggatat catctttgcc
     7141 ggttccgata cccgtaagca gctgaatatt gcagaagtca cagtgatttt agacaatagt
     7201 gatcattatt tgccattgga gtacaatgag atcagtgtaa cccgccgtta tcgacggact
     7261 ggggaaagcg agtttttcat caataaacaa agctgcagat taaaagatat ccaagaattg
     7321 ttcttagatt ctggattagg aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa
     7381 gcgattttca gtagtaaacc ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc
     7441 ttgaaataca agcagcggaa aaagaaagca gaacagaagc tttttgaaac agaagacaac
     7501 ctcagccgcg tccaagacat catccacgaa ttggaagaac agttaactcc tttggccgcc
     7561 cagagtgagg cagcaaagga gtttttacgg ctaaaagaaa cactgactca aaccgatgtc
     7621 tctttgatgg ttgccgaaat caagacagca aaaaaagatt gggacaacaa acaagcacag
     7681 cttgcgaaat tcaatctaga attaggcaaa ctatctgagt cgatccaaga acaagaatcc
     7741 attttggcga agcagcgtaa agaaaatgcg caagccgacc gtctgatcga aaaaaatcag
     7801 caagtattat tagatctttc ggaaaaactg aagcagacag aaggacaaaa agatgttttg
     7861 caggagcgga caaagcatac acaaaaaagc tcgcaagaat atcaaacttc attagcagaa
     7921 gcacaaaaaa aggttaaaca ttttgaaaag cttcaagaaa gcttgttgaa agaagcagca
     7981 gaaaaagaaa cagagatcca aaaagctgaa gcaaatctca taaaaacaca gcaagaatta
     8041 gaaaaatacc aaaaatcaac gaaagaatta ttggcagaat taagagatca atatgttgat
     8101 ttgatgcaag aacaagcagc tgtaggaaac gaactgaagt atctagaacg tcaatatata
     8161 caagaaacag caaaaagcaa gcagacgtta gccaagcaat cggaagtaga agcatcagta
     8221 gaccgattga tcttacaaaa agaagaattg acacaaaagc aggcgcagct aaaatcatct
     8281 ttaactgaaa caaaagagaa gttagaaatg atccaacaaa acgggaaaaa gttccaagaa
     8341 gtattagcaa aagaacagcc taaaatgtat caattgatga atcaagtcca gcagctgcgt
     8401 gctcgtcaga aaagccttca agaaatccaa gaaaactatt ttggttttta ccaaggggta
     8461 cgattggtct tgcagcataa acagcagctt tcgggtattg tcggagcagt ggctgaactg
     8521 atcgatgttc ctgcagattt cacgttagct atcgaaacag ctttaggtgg ggcagcccag
     8581 catgtcatcg ttgaaaatga aaaagatgct cggcaagcca tcacgtattt gaaacagcaa
     8641 agaggaggaa gagcaacatt ccttccactg acgacaatca aaccaagaca attaccggct
     8701 catatattga cacaagcagc agctgtagaa gggtttatag ggattgccag tgaacaagtc
     8761 agttatcccg atcaaatcca aactgtggtt caaaacctgc taggaacgat ccttttagcc
     8821 aaagacttaa ctagtgccaa tgcaatcgcg caaacgatcc actatcaata tcgagtggtt
     8881 tccttagaag gtgatgtgat gaatgccgga ggatccatga caggtggagc gaataaacga
     8941 ggaaaccaag gcagtttgtt tgtacaaaat caagaattga aacagttgac tagtgaattt
     9001 gaagaagcag ataaacaact ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa
     9061 acagctcgtt tagccgaaga acaagaagtt ttgcgtacac gaggcgaaca attacgtttt
     9121 gaagaacaag aagcgacaaa tcagctgcaa aacatcataa atgaacttga acgttttgaa
     9181 aaagaaaagc aaatctcaac ttttgagaca cgtgaactgc agcagtttat cgaagattac
     9241 gaaaagcaaa cgaacgaatt gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa
     9301 atcgacgaag aaatcaaatc gttaagtcaa gaaagcgacc agatggaagc cagaagagca
     9361 caagtacaaa gtcaaaaagc acaagaacaa gcagatcttg ctgtcttgaa agagcagttt
     9421 aatcatctgc agatccagct ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt
     9481 caagaagcga tagaaaagca gttggcgaca ttgacagcag acttttctga tcatgaagtc
     9541 acagaagaaa gtttggaaaa acaaattaac gaattatctg ctcagcgaga aacattaaaa
     9601 gccgagttgg caaaagccaa agagcagcga gatagaaggc aaaaggagat cgataagctt
     9661 gaagcagtct tggcagagcg taatcgtgag caaaaagcac gactgtcgga acaatccaaa
     9721 ttagaagtcc aaaaagaccg agctgaaatg attttagata atcatttgac ctatctgcag
     9781 tcagaatatc agctgacatt tgagaaagct tcccaagact atcaagaaac aaccgatatc
     9841 gaagacagta gaacaaaggt ttcatcctta aaagaacaga ttgaaaaact gggaccagta
     9901 aacttaaatt caatcgaaca atacgaacaa gtcagtgaaa gacatacatt cttagcaaca
     9961 cagcgagatg atctgttagc agcgaaaaac caattgtttg aaacgatgga tgagatggat
    10021 gatgaagtgc ggacaagatt caaagaagta tttgaagcta tccgtcaaga attcaaaatt
    10081 gtctttccaa atatgtttgg cggcggtaaa gctgaattag tactgacaga accaactgat
    10141 ttgctgaaaa caggtattga gattgaagta caacctcctg gcaaaaaact gcaaagtcta
    10201 agtttgcttt ctggaggaga acgagcactt acagccatcg cgctgctctt ttctattatc
    10261 cgtgtctgtt ctgttccgtt ttgcatctta gatgaggtag aagcagcgtt agatgaagca
    10321 aatgtgaaac gttttggtcg ttacttgagt gatttccaag atgacacaca atttatcgta
    10381 gtcacacacc gcaaaggaac gatggtcgca gcagatgtac tgtacggagt gacgatgcag
    10441 gaatccggtg tatcaaaaat cgtttctgta cggatggaag atatcaatga agaggcgaaa
    10501 atcaacgaaa cgaaagtgta ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg
    10561 gtacattatt aaatgaacaa aagctgattt cagatgagaa taaacaagca ttagcacaag
    10621 caaaacagca aggggtcaag atcgttctat gtacaggacg ccccttagct gcgatggcgc
    10681 attatctgca agaattagga ctggtagatg aaggtgacta cagcatcact ttcaacggcg
    10741 gtttggtaca aaaaaatgat actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag
    10801 atatccaacg cttatacaaa ctggctcagc aattggatct gccactagat gttttgtctg
    10861 acaatgtggt tttacagctg cctagtgccc caaggaagca atccttgtac aatgtgttga
    10921 ataagctgct gcaattccaa ccagcggata taagcgatat tacagatgaa tttgtgctta
    10981 ataaggcagt aatcgcttat gaccaagaag aattagatcc taaaatcaaa gaaataccag
    11041 cagaattcca tgaaatgtat gagatcatca aaacgagaag tatgcttttg gaatttatgc
    11101 caaaaggtgt tacaaaagct tatggtattt ctttattggc aaaagatctt ggtttgaaac
    11161 aagaagaaat catggcaatc ggtgatgaag aaaatgattt accgatgatc caatacgctg
    11221 gactaggcgt agcgatggag aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga
    11281 cagccagcaa tgaagaaaat ggcgtagcca aagtgatcca aaaatttgta ttagagtagg
    11341 ggggagtcaa atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac
    11401 agaagaagtt gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt
    11461 tggtgaacgg atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga
    11521 agaattagaa gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaatcac
    11581 cgaagcatta cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa
    11641 tgcgatcatc gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga
    11701 actaaatatc caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg
    11761 gaaaacaaca agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt
    11821 gatggcagca gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga
    11881 acgcgctggt gttgaagttg ttcgtggcaa tgcaggcggc gatccagcag ctgtcgtatt
    11941 cgatgctttg gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg
    12001 ccgactgcaa aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca
    12061 gcgggaatta ccagaagcac cccaagaaac acttcttgta ctagatgcaa caactggtca
    12121 aaacgcgatg gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt
    12181 gacaaaatta gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca
    12241 tttaccagtg aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc
    12301 aaatgatttt gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa
    12361 aaagcaggag ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc
    12421 tgtgtttttg atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt
    12481 ctttttctta gactttttga ttataattag attcatatga attggaggtc acttaatgat
    12541 gaattttaag caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt
    12601 tggtatagaa cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca
    12661 tccagcaatc tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac
    12721 acaaacagag atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc
    12781 tgctgtttat gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag
    12841 tatgccacct gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt
    12901 cgaagatgtc ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt
    12961 aagcggtatc catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca
    13021 tcaggaagaa tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagggag
    13081 aaactttatg cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga
    13141 gaagaactac tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag
    13201 tgcgctgggc tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta
    13261 tttagcagat atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta
    13321 tacaccgctt cgtttccgag gtggaaagaa agtcgcagat ttagcaacaa caggtgttcg
    13381 ttatattgaa ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga
    13441 gcaagttcgg ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga
    13501 ctgtgaccaa tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc
    13561 atcagatcaa acagaatttc atcaagaagg tagagaaatt cttgaaggga tgaagcaaat
    13621 gctggttgaa ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat
    13681 ggatcatcct gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca
    13741 gcaagaagtt gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta
    13801 tcagttggcc ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat
    13861 ccaaaaaggt gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt
    13921 ccaagaccat gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt
    13981 cccacttatc atggaaaaca aaacagtaac aaaaaaagtc ttaaaagaag ctgggttccg
    14041 ggtaccagga ggcgcagaat tttcatcaat ggaagaagcc gtaaaagctt atccgagatt
    14101 tgccgatcag gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat
    14161 tttcaaagaa ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga
    14221 agacagttcg gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat
    14281 cgacggagaa gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacggtat
    14341 ccgaacagtg aaagaactag ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca
    14401 tcgtgcacct ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca
    14461 agggttaacg atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc
    14521 aaatatcagt acgggcggtg attcgatcga tatgactgat gaattttctg aagcttacaa
    14581 aaaaatcgcc gtttctgcgg tagaagcact aggagctaag atcagtggta tcgatttgat
    14641 tataccagat aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc
    14701 aaatttcaat ccagcgatgc atatgcatgt ttatcccttt gcaggaaaag gcagaagatt
    14761 gacgatgaat gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac
    14821 agattgtcct atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt
    14881 gttttttcgt tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt
    14941 tcagctggcg aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt
    15001 actgcttctt ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg
    15061 ttgactacag taaatcggct attgtttttc gttgctagct ggatatattg gttttggaca
    15121 gaaaggggta gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt
    15181 aaaaagtttt ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag
    15241 cgttccctga atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca
    15301 taaagtccct tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt
    15361 cttctctttg tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa
    15421 aagcggttgc ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag
    15481 aaaggtggat tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat
    15541 ttcattgttc atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca
    15601 aagcttacaa atcttgctag cgactcgttt gccacgaacg atttgtttga tccttgtggg
    15661 agcgaccagt agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc
    15721 accaacgact gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt
    15781 cctacctggt gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg
    15841 aacattgctt tttttatccc ttacgcgatt gttcccccag aaaaatcaat tgatgcttcc
    15901 gttgattgga gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca
    15961 attccagttg gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg
    16021 aggaaattat gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc
    16081 ttaccgattt acgatcattg gtctgggcga atcgtttgca gtaaatttgg gaatagatta
    16141 ccagaagatg cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat
    16201 catggtagga agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata
    16261 tggagatcaa gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt
    16321 attttgcgat cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat
    16381 cgttggaatc atcggtgggg ctatcttcct ttatcttctg ataagggggc gacgatatgc
    16441 ttaaaaaaaa gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa
    16501 tgtacttgac gatccataca tatggaaact ggcattttgc atgggcattg aggagtaaga
    16561 aaatttttgc atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga
    16621 caattacgca taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc
    16681 ttgtccaaac ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta
    16741 caggtttttt cttattgaac gttgggctga tggtcgggtt gagtctgttg ttatttttct
    16801 ttcttttgaa aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag
    16861 gtacgctttt tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg
    16921 atttgcttca aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc
    16981 ctgcttcttt gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag
    17041 acgttttcca tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg
    17101 aatttttgat tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc
    17161 cagttacttt tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc
    17221 gtcatggtca gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac
    17281 agctcgcagt agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg
    17341 tcggaggaat ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga
    17401 tttacaaaag gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac
    17461 gattcctgaa aagaagttga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt
    17521 gttgtcgatg atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa
    17581 cgaagtaaag gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc
    17641 gaatgaaata aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta
    17701 tacgaaagga aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct
    17761 tgggttagaa gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg
    17821 tgcgtatatt gcaatggtac tagcgcaaga cacggattat attttactgg atgaaccttt
    17881 gaacaatcta gatatgaatt ttgcggttca aatcatgcag atcttaaaaa aattagtaca
    17941 agaaatcggg aaaacaattg ttatcgttct tcatgatatc aactttgctg caagttatgc
    18001 agacgaaatt gttgcgatga aagatggaca gttattcacg caaggaacga cccaagaaat
    18061 gataaaaaaa gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga
    18121 aggccgcaga ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat
    18181 tagtagtagg tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca
    18241 acaccagtca ggcagataca aagaaaagca gtacgcaagc tgaaacggca ttaacgataa
    18301 aagacagtaa tggcgatcaa atcgaggtgc caaagaatcc tgaaaaagtc gtcgtattcg
    18361 acaatggttc tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag
    18421 ctacgagcag ccttcctgag tatctttcct cctataaaaa agtggaatca gcaggtggga
    18481 tcaaagaacc agacttagaa aaaatcaatc agcttcagcc aaacctgatc atcatttctg
    18541 gccggcaaag agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt
    18601 tagatgcgaa aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct
    18661 ttggtaagca agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga
    18721 cgaaaaagaa agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc
    18781 aattatcagc ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg
    18841 aacaagcaga tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg
    18901 tgctggaaaa aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag
    18961 acgacagcaa agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa
    19021 atcagcagat catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat
    19081 caatgaaact gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga
    19141 ttaaactctc atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat
    19201 cctcaaagaa gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt
    19261 ttatttattt gggaaatctt ttttcaacgt gaattcattc tctacatcat acgggttgat
    19321 tctagaatgg ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc
    19381 ctttgatttg aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt
    19441 tattttgtac cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc
    19501 cactagacta gaaaatatga gaagctattt tctgtccagc gttcttatta cttaaagcaa
    19561 atcgtagttg tcaaaatctc ttctaaacga tgttccatca gctgaccttc ggtattagct
    19621 caaagcactg atttcacaac ctctatttag gcatgtgtct cagcaagcgg ttgatagttg
    19681 atgatctgca tttttcgata aaaatcggcg ggaagcttac tccaccgttt taataattga
    19741 tagaaataac tttcttctgc tttgatttta tactcgctta atatcaatcc aaaactattc
    19801 gttttttttg aagctaattt tttataaata tccaatgctt tggttttatc ttcattgaca
    19861 aagagtgttt ttacttcaat ataagaaccc tcgatatttt gtgtttcttc gataaaatag
    19921 atgtttttta tatattcctt catagtgatt cctccaaaga agtcttttat ttgataagtt
    19981 tactctaccg aagagttgtt aataatccgt gaacaaaaag taattctttt tccaatagtt
    20041 gttaaaggtt ttttattttt tatagtattt tttgttattt aagaaaggaa taaggttaaa
    20101 caaataacag gttgcttttt gggtcttttt ccgttcaaga aactgaacga agaaaaggga
    20161 aagttgtctc ttttgtgata agagaaatca taataaacga atgacttact ttccaataaa
    20221 ttgagaacag tcagaaccag acaaattagt tgcatctgat gaataaagtg attattctat
    20281 gacatgaggt gatacagatg aaattagaag tgacaaaaga agcacaagaa gtattgaaga
    20341 ataaattaga agcagacgat caacttttat tagatatcga aaacggtgac ggtccatttg
    20401 cagaaagcca agtcagttgt caattagaca cggcatttcg attgatcatt gttaaaaaag
    20461 atacacaaac agatctaagt ctttattctg tagtggctga tacaccagtt ggaccaatca
    20521 aaatcaaaga cagtgcaata ctttacatgg atgatccatc cacacttgct ttagaaccaa
    20581 cttataatag tttacaatta aaaggaccaa gtggcttgag aaaaggcaac ttgcaagttg
    20641 ttcgaaaaga ctagagggat aaaatgggaa atttggcaat catcagcgac ctacatgtgg
    20701 acatcaataa attcggcgaa ccagagctaa tgcagctttg gcaagttttg caaaagcaca
    20761 aaatcactcg gcttcatcta gccggagata cggctaataa agtagatcgc tgcctagcgg
    20821 ttgtcgaatt tttcgccaat aaaggattat ctacgacatt tcatttcggc aatcacgagt
    20881 tggcagatgt gcccaatgaa cagatgatgg accattttcc agacccacat tttttaaatg
    20941 aacggtatat tgctttaaat gagcagaccg ttcttttagg gatgaatggc tggtacgact
    21001 atcagttttc cgaattgaaa gattacgagg caatcgtacg aatgaagcgt ttgtattggt
    21061 atgatcggtt catccagcgt gggaaaacag atctcgaggt caatgcagaa atgcttgcag
    21121 ctgcaaaaaa tttgctagac cagctggaag caaaaaaact tgaggtgatt ttatccactc
    21181 atttcgttcc taaacgggca tttatcatgt atcaatctgc tccttatgag cgttggaaca
    21241 aattaaatgc atttttagga tcagaatcat tcggagaatt acttgaccgt tattcaaata
    21301 tcaaacaagt agtttttggc catacgcatc gtcgctttga agataaatcc atcggtcaca
    21361 cgatttacag ctgcagacct tttggttatt attatgaatg gcagctgacc cgagattttg
    21421 tcttgaaaga acagttgcta gacagctatg atcctatgaa attacgtgtc ctcttgcgtg
    21481 cccatcaccc agcttttcag gcctatcaaa cacaacattt gcaagaggaa tttgaacaag
    21541 cgatgacaat cgttccgtat tgagacagca ttagtaatgt gtaaaagaaa ataccttgta
    21601 aaacataagg ataagtaaga gcagtagatc catttatcgt ggatatgctg ctttttttgt
    21661 ggaaaataat cagcgaatgt ttatttgatc ggatgatttg taactcgcag agtactagat
    21721 gcaagcgttt attctttttt tgaacgataa tcacaaaaaa aaatataata tacatttttt
    21781 atttaattat aattaatatt gattgaagtc taggcagtca agtcacattt tgatagatag
    21841 gagggggact tacgaattat tttaaaggct atgacagctg tatttctaga ttgtgtttgc
    21901 atcaagttgc aataaaaact agaaatcaaa tcgggctgat aatcatttat aactaatcga
    21961 tacataggac caaatatagg tctatgttct tttatttcat aagtatcgta aaacagaatt
    22021 ttgctatgga ttaatccaat cgactgatat gtttagtaaa taaagaaaga ggaatgtcaa
    22081 tgatgaaaaa agaaatcgta ttaacagcag caacgatgtt attcagcatg gtagcaagta
    22141 cgacttttgt ttctgcaact gaagtatacc caaaggaata taacacagaa ggaacgatta
    22201 catttgaagc aggagatgag ggtgtaactc ctcctgttga tccagagaat cctgatccca
    22261 ataaacctgt cgatccatca gatcctccaa gccctggacc aggaggggca ttatcgattg
    22321 attatggatc aaaattcaaa tttgggactc aaaaaatttc tactgcggat aaaacgtatt
    22381 atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc gacatatgtc caagtgacag
    22441 ataggagagg taccttatct gggtggaaat taagtgtctc tcaaccagaa cagtttaaaa
    22501 ctgccagtgg ggatgaacta gttggagcac agctgaaatt tacgaaaggg caggctgttt
    22561 ctttggttga tccaacatat atgccgcaaa cggtgaattc tgaattaacg ttgacaccgg
    22621 gaggaaacaa tacgttagca attaatgcta aatcaggaac aggtgtgggg acttgggtct
    22681 atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt acagctttcg gtacccggta
    22741 aatctgtcaa gttagcgcaa caatattcca ctaaattagt ttggacatta gaagatacac
    22801 ctaacaacta gtttactgaa aagaactgtt aaaaataaat acgtagtcat attgtcttgc
    22861 gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt tgtttggata cgaatagttc
    22921 atctaggaat ttaacgatca ggaggaaaag atatattgaa aaagaggagg atcgtgctca
    22981 ttttaagcag tctcttagtg actaatatct ttccgatcat agggtactct ttggaattga
    23041 atcagttgga gcatggaaat agaagagacg aagaaagcaa actcacttcg acattcgaca
    23101 gtttaataga atccaaccag acacaagaaa gcagtgagga tttaagtatt tcgagtagtg
    23161 gagagaccac tgacaacgta gaagctgagg gttcggacaa acaaggactg gaagatgcac
    23221 caatttctgt ggttgagctg cctgacgatc ccaatattat ttctatcgat aaaatttttc
    23281 aaacacctat cggtactagc acgtccatat tggaaaatgg gaagttactg cagttgaacc
    23341 cagcagaaag gtctcaaaga ggagctattt ggtcaaataa gaagattagt ttactctcgg
    23401 attttacatt taaatcttat ttatacttag gaaatcaacg agatagagcg ggagatggca
    23461 tgacctttac attgactggc gatcctagaa tggctagtaa tcccagtcaa gttattggat
    23521 cgccaggtat gggaattggc tcatattcta ctcgttctgg ccaaccttac attgcgaatg
    23581 cgctctcgat tgagtttgat acgtataaaa atagtggttc ttctgatcga atggatcgag
    23641 agataactgc tgataataga gggcatggac atgtcgcttt tgtcacgcct aaaccaaata
    23701 ataataatta ttctggagag catactggcg tgacggtagc tcctacattt ctttctaatg
    23761 gcacatggcg cttgttaata attaaatggg acgcggagca aagaagtctt acttatgatt
    23821 tggaaggtgt cggtcgctct tctcggatta tacctgatct cattaggcag tttggttcta
    23881 cagaagtaaa ttgggggttt acttcttcaa caggtacttt ttttcaagaa aatgctgtcg
    23941 ctgtcacgcg acttccatcg aatgtcacat caacagctga tgtaaaagtc aacgatagtg
    24001 attatggggc catcgctgaa gctggaggaa acgataagat aactatcaga aatacattga
    24061 aggtcgaggg ggattttctt gaatataaga aaatgcagac tagtatcgac ctcccacaag
    24121 aattaactat tcccgagcat atcttgatca atggaaatca aattgatgcc aaagatatct
    24181 tgataaccgg aaatcgagtc acgctaaatc tgacagatta ttttattgct ggagatacgt
    24241 taataatcga attggagaca actttgcagg aagtaattcc tgaaaaagta ctcactatgc
    24301 attttgaata ccttgaaaac gagaatttga ttgagcaatc gaatgaaata aacctaaaga
    24361 tcgcaaagct gaaggagata gatatccatg tttattataa agataaagac agtcagattg
    24421 atattgctga aatgaaaaca atcactggga aaataggaga gaagtataaa gaaactccta
    24481 ttgatgttga agggtatgtc tttgactcgg attcagcgaa tacagaaggc atcttctccg
    24541 aaaatacaga ggatatctat ttttattaca gaaaaggcaa actttatctc tcaactgctc
    24601 ctcaatatgt caattttgga aaacataaaa tatccagtag accattaagt gaattcggac
    24661 agttttcagg tgggttgaaa attatagatg aacgggcttt tggaagttgg aaattacaat
    24721 tgaaacaaga gaaagttctt acaaatggag attttgagct tccagaagct ctttcctttg
    24781 ttacctctga aaatacaacg gttataggga catccgctgt tacgattttc gaaagtgatc
    24841 aaaagggtga aagcgatttg tcctctcttt tagattctac agctcatcgt ggaatcagaa
    24901 tagatattcc tgtagaaaac caaagaatcg gaacctttga ggggaaacta tcttggatac
    24961 ttgcagatgt cccaggaaat taacaggaag agaagaatat aacaaaatta ttgagtttca
    25021 agaagggcac aggtaccata ggctcttgtg tcccacagag acaagatttt gttatagtct
    25081 tctcttacat atgattaaag gagcgatttt cttgagacca aaacaacaat tatttataac
    25141 actcctagtt attttagggg cactttttaa caatatatca gtgtcagcta gtgcatttaa
    25201 tttttcagtg acgccgataa cttcagagaa tcaaatcgac aagagaaaaa cttacttcga
    25261 tcttcaactt gatccggatc aggaagtaga agtaaaagcg gaattgagaa atgatacaga
    25321 aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc accaattcaa atgtgatggt
    25381 tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg atctttgatc tggtagatta
    25441 tgtgagctat ccacagatag tgacgctaaa acctaaaagt gtccaaaccg ttgcttttca
    25501 tgtcaggatg ccgaataagc gatttgatgg tgtattggct ggtgggatca cattccaaga
    25561 aatccagacg gaaaaggacc aaacagaaac taaggatcaa agcttatcga ttgagaatga
    25621 atacacctat attattgctt tgttgatgcg acagacactg aacgaagttg ctccgaacct
    25681 tactcttcat taagttaaac cagatcaaat caatgcaaga aatgtcatct tagcaaacgt
    25741 tcaaaatgat caaaaaacct atatcaatca agtagttatt gaaacaaaaa ttacaaaaaa
    25801 aggacattct gaagttttat atcaagagga aaaagaagcg ttgcagattg cgccgaatac
    25861 aaatttttct tttccaactg ctttaaatgg tcaaccgcta accccaggtg aatatcacct
    25921 tacaatgact gttttaggaa atgaaaatga aaatggaaaa ttttcgagaa aaaaaggaaa
    25981 tactacgatc aactatacca atcaatgggt atttgaaaaa gattttacaa tagacgaaaa
    26041 agtagccaag gaattgaaca cgacggatgt tacgattaaa aaagacaata attggattta
    26101 tatcttagta gcttcacttt tcttattgtt tattttatta tgcatacttt
//
LOCUS       DACDVT010000032        25501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDVT010000032 DACDVT010000000
VERSION     DACDVT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.032"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWL52_001401"
     CDS             133..3660
                     /locus_tag="IWL52_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9777972.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWL52_001402"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWL52_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9777973.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWL52_001403"
     CDS             7448..7789
                     /locus_tag="IWL52_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9777974.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWL52_001404"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWL52_001404"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9777975.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWL52_001405"
     CDS             9146..11566
                     /locus_tag="IWL52_001405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9777976.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWL52_001406"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWL52_001406"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9777977.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWL52_001407"
     CDS             13000..13737
                     /locus_tag="IWL52_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9777978.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWL52_001408"
     CDS             13751..14566
                     /locus_tag="IWL52_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9777979.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWL52_001409"
     CDS             14579..15226
                     /locus_tag="IWL52_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9777980.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWL52_001410"
     CDS             15242..15898
                     /locus_tag="IWL52_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9777981.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWL52_001411"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWL52_001411"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9777982.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWL52_001412"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWL52_001412"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9777983.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWL52_001413"
     CDS             18240..18758
                     /locus_tag="IWL52_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9777984.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWL52_001414"
     CDS             18865..19365
                     /locus_tag="IWL52_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9777985.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IWL52_001415"
     CDS             complement(19645..20565)
                     /locus_tag="IWL52_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9777986.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IWL52_001416"
     CDS             20730..21743
                     /locus_tag="IWL52_001416"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9777987.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWL52_001417"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWL52_001417"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9777988.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IWL52_001418"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IWL52_001418"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9777989.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IWL52_001419"
     CDS             complement(23383..24144)
                     /locus_tag="IWL52_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9777990.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IWL52_001420"
     CDS             24333..25427
                     /locus_tag="IWL52_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9777991.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8449 a         4424 c         5382 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattacttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 g
//
LOCUS       DACDVT010000033        25471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDVT010000033 DACDVT010000000
VERSION     DACDVT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.033"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            152..1621
                     /locus_tag="IWL52_001421"
     CDS             152..1621
                     /locus_tag="IWL52_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9777992.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTGNPKNIRLFL
                     YNLFNSTEISFVELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAEQAD
                     SLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETI
                     EWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFREN
                     YPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSY
                     GAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRA
                     VDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1714..2448)
                     /locus_tag="IWL52_001422"
     CDS             complement(1714..2448)
                     /locus_tag="IWL52_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9777993.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            2774..3184
                     /locus_tag="IWL52_001423"
     CDS             2774..3184
                     /locus_tag="IWL52_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9777994.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            3177..3878
                     /locus_tag="IWL52_001424"
     CDS             3177..3878
                     /locus_tag="IWL52_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9777995.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            3878..5491
                     /locus_tag="IWL52_001425"
     CDS             3878..5491
                     /locus_tag="IWL52_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9777996.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            5481..5702
                     /locus_tag="IWL52_001426"
     CDS             5481..5702
                     /locus_tag="IWL52_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9777997.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            5699..6460
                     /locus_tag="IWL52_001427"
     CDS             5699..6460
                     /locus_tag="IWL52_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9777998.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            6841..7350
                     /locus_tag="IWL52_001428"
     CDS             6841..7350
                     /locus_tag="IWL52_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9777999.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(7420..7959)
                     /locus_tag="IWL52_001429"
     CDS             complement(7420..7959)
                     /locus_tag="IWL52_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9778000.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(8099..8710)
                     /gene="rpsD"
                     /locus_tag="IWL52_001430"
     CDS             complement(8099..8710)
                     /gene="rpsD"
                     /locus_tag="IWL52_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9778001.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            9026..9823
                     /locus_tag="IWL52_001431"
     CDS             9026..9823
                     /locus_tag="IWL52_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9778002.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            9851..10486
                     /locus_tag="IWL52_001432"
     CDS             9851..10486
                     /locus_tag="IWL52_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9778003.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            10506..11279
                     /locus_tag="IWL52_001433"
     CDS             10506..11279
                     /locus_tag="IWL52_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9778004.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEKQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            11687..12484
                     /locus_tag="IWL52_001434"
     CDS             11687..12484
                     /locus_tag="IWL52_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9778005.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            12462..12875
                     /locus_tag="IWL52_001435"
     CDS             12462..12875
                     /locus_tag="IWL52_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778006.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            12859..15504
                     /gene="mutS"
                     /locus_tag="IWL52_001436"
     CDS             12859..15504
                     /gene="mutS"
                     /locus_tag="IWL52_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9778007.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            15522..17630
                     /gene="mutL"
                     /locus_tag="IWL52_001437"
     CDS             15522..17630
                     /gene="mutL"
                     /locus_tag="IWL52_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9778008.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     LQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            17652..18212
                     /locus_tag="IWL52_001438"
     CDS             17652..18212
                     /locus_tag="IWL52_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9778009.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(18297..20285)
                     /gene="treP"
                     /locus_tag="IWL52_001439"
     CDS             complement(18297..20285)
                     /gene="treP"
                     /locus_tag="IWL52_001439"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9778010.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            20508..21221
                     /gene="treR"
                     /locus_tag="IWL52_001440"
     CDS             20508..21221
                     /gene="treR"
                     /locus_tag="IWL52_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9778011.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            21298..21744
                     /gene="msrB"
                     /locus_tag="IWL52_001441"
     CDS             21298..21744
                     /gene="msrB"
                     /locus_tag="IWL52_001441"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9778012.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            21914..22516
                     /gene="ruvA"
                     /locus_tag="IWL52_001442"
     CDS             21914..22516
                     /gene="ruvA"
                     /locus_tag="IWL52_001442"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9778013.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            22529..23530
                     /gene="ruvB"
                     /locus_tag="IWL52_001443"
     CDS             22529..23530
                     /gene="ruvB"
                     /locus_tag="IWL52_001443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9778014.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            23559..24137
                     /locus_tag="IWL52_001444"
     CDS             23559..24137
                     /locus_tag="IWL52_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9778015.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            24189..24671
                     /locus_tag="IWL52_001445"
     CDS             24189..24671
                     /locus_tag="IWL52_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9778016.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            24788..25162
                     /locus_tag="IWL52_001446"
     CDS             24788..25162
                     /locus_tag="IWL52_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778017.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         8376 a         4523 c         5403 g         7169 t
ORIGIN      
        1 ctctccaaaa tgttaaaaaa aagacaggac gatgtatatt atacaaacaa gaatcgctga
       61 caacagtgac tcggatccag gaaatcagtt ttatccccag ctagttggtg aatgcatgga
      121 aagtaatata cgtagaaaga gctgatgact aatgaattat gagttttttt taagcaaaga
      181 agatcacaga agttattcat tacttaaata tttagaagca agctcaaatt tatcagagtc
      241 aatatcgaat gttcaagaag agttgtcgct atcgacattt ctcttgaaaa aaacgatcga
      301 taagctgaaa catgatttac aaaagttcga tctagagaaa aatttgcgag taaccgtcag
      361 tgaattggat atatgtctgg aaattaatgg aaaatgttct agcaaagctc tgttatctcg
      421 ttatataacg gattctttat ctatgaaaat ggtattggct ttctttcagg aaaaatatat
      481 ctcagtagaa aattttgcta aacagcatca cgtgagttat tctgtggctt ataaagtatt
      541 acaaagacta aaaagaaatc ttaaaaaata tcaaattttt tttgaaaaga gaaagttgac
      601 agggaatcct aaaaacattc ggcttttttt atataatctt tttaactcaa cagagataag
      661 ttttgtggaa ttgtatacgc tacctataat tataaaaaca gaaaaaatgt taaaagattt
      721 aaaacagtat tatcctctgt ctgcttatga aaaaaagaaa ttattccact ttttagcaat
      781 caacctgttg gctgaacaag ctgattcttt agatattgct agcaatcagt cgctattttt
      841 ttcggatata ggattttacc acttaaaagg agtattagga gaaaaatccg ttatttttat
      901 caataagaca ctctcttggc tttatcttca tgataaattg gatcaaaaat acttgagaaa
      961 aaatgaagat gaaacaatcg aatggttgaa tgaacgattc attgaaagtt ttgaaaatca
     1021 gtttaatctg ttgcgagaag agacgagcca attgatccga agagagttaa ccaaaataca
     1081 ttttaaactt ttgcattaca ctattaataa agctgatgaa tttccacttg atgtttcttt
     1141 ttttagagaa aactatccag cattttattg ctatttgata gagtatataa atgatctaac
     1201 gtcaaaacgc aaagagttgc ttcaagataa attttttctt tttttcagtt atttattatt
     1261 attgatcaat cacataccca ttcaactgat ttctgaacca gtaaagatca tcatcgattt
     1321 ttcttatggt gctgcctata atcaatttat caagaaaaat ttaagcttgt acattaatct
     1381 gaacacggaa gtgatagagg caggaaagcc tgagttcccg gatgttataa tcacgaatag
     1441 gaataatcta tatgaagaaa gtgcatctca agtagtggtt tggctcgatc cgccaagagc
     1501 agtagactgg gggaatctta caaagatttt gttgactatc caagaagaga aatacaaaga
     1561 cagcaaaatt gacaagcaaa tagacgattt tcctgaagaa atcatcgaca ggatcgaata
     1621 gtgaactttt tttagaaagt ccgactgctt tttctaaaaa taaaaaaact tggtacgcaa
     1681 tcgctgtacc aagttttttt atttagcttt tgattaagcc ataaataata atttatataa
     1741 gaatactgct acgacaccag cacagatagg tgctacaact ggtacccatg catatcccca
     1801 ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt acaagacgtg gagcaaagtc
     1861 gcgggctggg tttagtccag gacctgtagg accaccaagt gaagcaacta atgtccatac
     1921 aaggaaaccg agtgctaagt gagctgtacc aagattatct tggaagaatg gtgcttttgt
     1981 aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag aattcattga cgaaaccatt
     2041 gaatttagaa ccaacgctgt tgattgttga gaatgtacct aacacatggt tcacgtcttc
     2101 tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc aattgtccaa tcgctgcacc
     2161 aatcaattgt gcaatgatat aaggaacaac ttcagcccaa gggaatagac cattagatgc
     2221 taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg attccaccaa aaatcattgc
     2281 tggcatcata accccgaatc catacccgac agcaatcagc atccagtcac tgccatatcc
     2341 ttttgtacct tttaaatcta cgtttgctac agcaccgtta cctagaacga ttagtaatgc
     2401 tgtaccaatc atttccgccg caagacgggt cattagtgag taatccataa atacctccaa
     2461 aagttataaa aatgcaaaaa aaaagaaaca tcaacacgaa aagcatcggt gcagtgtttc
     2521 gtgtcggaag gatttctgca gtcttaacaa atgtgaataa aaaaatgaat tccattactt
     2581 ataagaaacc agaccaacag taaaactcca actcttttta aaagttctcc acaaatcacc
     2641 aagcttaact ataaatatac caaaaaaaaa acgttttttt tctcaaaaat tgtatcttag
     2701 aaacagcatg ttttgaaact ttcagaaaag gtttgttagt aaaatgaaag ctatacgtaa
     2761 ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt attattttct tttctgtatt
     2821 aggagaaggc atacggattt ttgctcattt acctattcct ggaagtatct taggtattct
     2881 ttttttattt ttagcatttg aaatgcatct tgttgatcct gaaaaaattg gagcaactgg
     2941 tgatttttta ttgaataatc tagctatttt atttgttcct gctggtgtag gtttattgga
     3001 atattttgat gatattgctt cgatatggcc tgttttactg ggagcagtcg tcgtgtgttc
     3061 catcgtcacg atggttgcag cgggaaaaac ggcagaaggt gtagaggctt tgctgggcta
     3121 tgtccgtaag aaaaaagcag gaagggtatc tgtaaacgaa atggaggaaa atcaaattga
     3181 ctgagttttt tgtgaatcca ctatttggct taagtttgac tttaggagtg tatgtattat
     3241 ctgataaatt tttgcggaaa tataaactgc ctgttttgaa tcctctcgta ttatcgattg
     3301 ttgttattat cttatttttg catttttctg ggatcagcta taaagactat aatatcgggg
     3361 gcaatttttt gactatgctg attactcctg cgacagtggc acttgccatc cctctttata
     3421 aaaacttccg tttactgaaa gagaattttt atcctgtaat ggctgcgatt ttagcaggaa
     3481 tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt cttctctctt cataaagaga
     3541 tggtgatctc attactacct aaatctgtaa cgacggctat ttctgtcgat ttatctcata
     3601 cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt ctctacaggt atttttggtt
     3661 cgctgatcgc cactcatatt ttccgcctat tcaagatcga aagtccagtt gctcgcggtg
     3721 tggcgttagg aagtacttct catgctatcg gtacagcaaa agcaatcgag attggcgaaa
     3781 tagaaggaat catttccgga cttgcgatat gcgtaaatgg gatattgacc gttttgttgc
     3841 tgccgttatt ctttcagcct tttgctggct tgttttgatg cgaaaaccac tattttatgc
     3901 tacaataacc aaaacgttcg agcataaaaa aggagggaaa gacgtgtatt tgtcttccga
     3961 gtatcttaaa caatttgata aagatctata ttataagatt ctcctcctag aaagttttga
     4021 ggatcaagct tggcataccg ctgcacaatt agcgcaagtt gttcagctgg atgcccgcag
     4081 cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag cagtttagcg gtaagacaca
     4141 ccctttgttt acaaaaaatc accgttctgg ttataacttt tatgatacgc tcgattcaat
     4201 tgaacatgag cgttttttga tctatctcgt gcaaagtacg ctgaaattcc agttgctgca
     4261 cgacattttt tttgaagaat tccatacaat gtaccaattt gcgcaaaagc attacattag
     4321 tgaatctact gctcatcgaa aaatcaatga atggaaacag caactgcaaa cttacggaat
     4381 tcggctgcaa cgaggtacat atatcgctca aggcgaagaa gaaatcattc gactttactt
     4441 gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg ccatttgaaa cgatttctca
     4501 aatggacgta aagaatatgg cagaacatat catggctttt tttaatgttc gtctgaacga
     4561 gatcaaaaaa agacgattgg aatacatgct aggtgccttt tttttaagaa aatcacagaa
     4621 gcattatgtt gtattgaatg agaaaaaaag acggctgatt tctgacaatc tgttgttcca
     4681 gcgattttgc caagtcatgg agccggtatt tcctaattat tttcaagtag aagacgagtt
     4741 aggcgcatta tttcttgtac tgatgactcg tgaagaatat tatagtgatc ctaagatacg
     4801 gaagaaaatt tttgattttc atcaacaagc aaaaacaccg ccctttacag ccctttcaga
     4861 agcaaaagcc gctctttctc tttatcagga agagcagggg ctgccagcag aaaatctaac
     4921 cttcgaggca gaaaactatt tattcagcag tcattttttc gcctatcttt ttcctaatgc
     4981 gaaagaaacg attgatggca atagtagtga ttttattaat catttagtta ttgaaaacaa
     5041 ggaattgaaa caatggctcg tacatttttt tgaaagtaga cacaaacatc ccaatcatct
     5101 ggcatttaaa aatcatgcgt ttttgatggg tcggtatctg actgtgttta aaacattagg
     5161 agcctttact ccacagcttc caaagatcac gatcctttta atgacagatt tcccattatt
     5221 tgaagagcaa ctgttagagg agggattacg gacatttttc cgtaatgagt atcagctgat
     5281 ttttctgcca acagattatc gggggagaga agttgatctt ctgatttcga caagtaaagt
     5341 ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg accgaagaac taaaattaat
     5401 tgattacatc caactatctc aaaaatttga aatgattcaa aaaaaaacta ttaaaacaat
     5461 aacaaagaag gagaaaaaag atgacatttg aagaaatttt accagaattg aaaaaaggtg
     5521 caaagattat tcgtaaagga tggagtggat ttgaacttta tgttgttctc gtatccgatg
     5581 atgaatttga tggctgtcca gtgacacctt acttattgat caaaacaagt gacgaaggtt
     5641 tttccagctt cgctcctaca gtttgtgata ttttagcaga tgattgggcg atcgtagaat
     5701 gaaaaaccag tatccagatc tagctaaaca ggttgtattt atcacaggag ctgcttctgg
     5761 aattggtgct gcgcaagccc gagcgtttct tgaacaagat gcttttgtat ttggattaga
     5821 tcaaaagttc ggtaaaatgg aagaactctc tttgcgattt cctgaaactt ttgcctacgc
     5881 actaggtgat gttcgacaaa tgaaagaact ccaacaagcg gtatcgaaat gccaggatcg
     5941 ttttggtgaa gttacgattt tactgaatac atcggggata ttggatgcct acaaaccatt
     6001 gatggaaact gacgaatcgc tgtgggatct tatttatgag acgaatgtga aaagcatgta
     6061 tcagctgaca aaaatcatcc ttcctgatat gatccgccga aaaagcggaa caatcatcaa
     6121 catggcttcg attgcgggtt tggttgctgg cggaggtggg atcgcttata caagtgcgaa
     6181 acatgcaatc gtcggattta ccaaacaact cgctctggat tacgcaggac agggtatccg
     6241 agtaaaagga attgctccag gagccatcca aacaccaatg aatgcggctg attttgaagg
     6301 gaaaggtgag atggctgagt gggtcgcaaa tgaaacacca gtcaagcgat gggcacagcc
     6361 ggaagaagta gctgagctga cgttatttct tgcctctcct caagcaagct acatacaagg
     6421 aacgatcatc ccaatcgatg gcggatggtt gttaaaataa ccttgtgttt ttttcaagct
     6481 tctgtatgat aaaactaaag agagaaacaa cggttgatga taaaagaaag tcgatgactg
     6541 ttcccgacat tcgatgccga acagccagtt caaaaaattc tttttaataa tcgttaatat
     6601 tcgaaactaa gcggcttttc acgacctttt gatataaacg gtgttcaaaa gcttgcaact
     6661 gcggtaacaa gtccagccaa atgaaaaata taaggagcta tttttatttg actattctta
     6721 ttacttgttg cagggcagct ttttcactac cttctgcggc acttcagttg gaattttctt
     6781 cccatccttt ggtcgcagtc attaccaaga taaaattatc ttgcaaagga gaaatagaac
     6841 atggaaaaaa ataatacccg ttcagccaaa cgctggacaa cgacagatgt gactcggatg
     6901 gcgctagtca ctggtttata tgtggcagtg acgctggttt tgtctgtctt tagctttgga
     6961 gtgattcaga ttcgtctctc ggagatgttc aactatctgt ccttgtataa caaaagatac
     7021 atcgtcgcgg taacagcggg agtagctttg gctaatatgg cttctcctct tggactgata
     7081 gatgttgtag taggaagcgt cagcacgctg cttgtattgc ttatcaacta taaaatcact
     7141 agtcgcatca aaaacatgaa aatcaaaatg gctgtgacag catgcgtatt tgctttttcc
     7201 atgtttacag tagctggtca gctaacgatt ttatatcaac tacctttttt cttgaattgg
     7261 tgggtgattg ctttaggaga attggcatca atgattgtcg gtgggataat tatttattgg
     7321 atcggtaaaa aagtggattt gtcaaaataa agatctgttt aatttaaaat atggcagcgt
     7381 ggaagagact ttgagtcttc ttccatgctg cttttttgat taatttgtat gaagaaatcg
     7441 atttagccgt tttttcatca agataccgat ataagctgct ccagttgcct tgattgcttc
     7501 cggcagtaaa aacggcagta gtccactggc aaatgcagtt gtaaaagtaa gatcaccact
     7561 tattttcagc cagatcgttc cgcagatcaa tgcagcaaga gagcccatta gattggcaaa
     7621 gattgcatat aagtaatgag catccgttct ttcgatcatc caaccagtta tccacgtttg
     7681 taagagaaat ccaatcaaat atccgccagt tggcccaaat aaaacagcta tacctccagt
     7741 catccctgaa aatacaggaa gaccaatcaa gccaagtaaa agataaactg caacagatat
     7801 caatcctagt tttcttccga gaaaagttgc tgttaatcca atagcaaatg tttgccccgt
     7861 caaaggaacc aagccaaaag gaatggtcat ctgagaaagg acagaaataa tcgcggcaaa
     7921 ttccgcagca agtattaatt ctttcaatgt tcggttcata atgccctctt ttcgttaact
     7981 taatattttt atttggttaa cgaaattgta aaacaaagtg attactttgt aaatagaaaa
     8041 tataaaaaaa gaccagctat ccaatagata gcggtctttt tcaagaaagc ttttgaaatt
     8101 ataagctttg gttgtagtat tcaacgacaa gagcttcgtc gatttctgga tatagttcgt
     8161 cacgttctgg taaacgagtc aatgaacctt ccaatttttc agcgtcaaag cttacgaatg
     8221 ctgggcgtcc aacagttgct tctacagctt cttttactgt tacgatgttt tgtgattttt
     8281 cacgaactga gatcacttga cccacttcta cgtggtatga agggatatca acgcgtttgc
     8341 catcaactaa aatgtgacca tggttaacta attgacgtgc ttgacgacga gtcgttgcaa
     8401 gtcctaaacg gtaaacaaca ttgtctaaac gttgttctag taagatcatg aagttaacac
     8461 catgtttacc ttctttgatt ttgctagctt ttacgaacaa gttacggaat tgacgttcgt
     8521 tcattccgta catgtgacga agtttttgtt tttcagttaa ttgcatgccg tattctgatt
     8581 gtttgccacg gctgttaggt ccatgttgac ctggtgcgta agggcgacgt gctaattctt
     8641 ttcctgtgcc tgacaatgag atgcctaagc gacgagaaat tttccatgat ggtcctgtat
     8701 aacgagacat taaaatttcc tccaatgaat ataagttttt ggagtaaaat aatccgttga
     8761 aaaattcata atcatatagt ttgttcttca atcttcacct ttgcagccgc ggttacacaa
     8821 tcgaacccga gacggggcaa caaactaggg atgagttatt atttttctgc tgcattattt
     8881 tacacaaact acagtatatg atagatcatc cacgaatgtc aagataatta gtacataaaa
     8941 acgctttaca aacctaggct gtctttataa aatgagaggg tatgtgcaaa tggaaaaaag
     9001 cgaaggaagt ttaaggagaa aatcaatgaa tccggtatca ccattatttg agaaaatcga
     9061 tgcttcaatt gaaaagaagg cgaatctttt tgaaaacagc ttttcacgtt acgctgttcg
     9121 agcggttctc gcctgtttat ttttgacttt aggaacggct gtggcatttg caatcgcgat
     9181 gaagggggaa gatatcgcgc atggtctagg aaagatttta tacgcattta tgttcagctg
     9241 gggtctggtc atgattctat acatgaacgc tgaattagga acatcgaata tgctttatat
     9301 gaccgtgggt gtttttcggc agagacttgc tttaagtaag gcagccaaga ttctatttgc
     9361 ctgtattttt ttcaatatga ttggcggtat attatgtgga tttcttattt ctatgactac
     9421 gccatttcaa gatttaacgt tggacaactt tatgttagaa tcaatagcag taaaactagc
     9481 aaaacctgca gcaacgattt tggtggaagg gatgtttgcg aatatcgttg tcaataccgc
     9541 tgtactgatc agcatgcgga tgaaagagga tgccgggaaa gtgatcacag tcgtattcat
     9601 cattttcatt tttgcttttt taggatatga acacgtgata gcgaattttc ctgcctttac
     9661 gcttgcttac tttgcttctc aaggacagac ggatggaatg acagtcagca atgtgttaca
     9721 taatttgttt tttgctttgg caggaaatta tattggtggc ggtttagtaa tgggactggg
     9781 atatgcttgg ctgaatcagt caaaatcatc ctatgtggac taaaaagtcg ttcattaaag
     9841 gggaagtgcc atggaagttt tagtttttcc aaaaaatgag gaaaaagcgt tacaaatgga
     9901 aaagtacatg aaaaatcttt tttcctttgc tggagtacct aagccggaac gcgctctttt
     9961 acaacaaccg ttactggatg agagcaaaaa tttatctatt tctgaagttt tagcttttgt
    10021 ttattattat tatgaacaaa tcgaaagaga gtatcagtat gtagcgatcg atttagtcca
    10081 gaaaaacatc aaacggctta catttgaaga cttagtcgta ttgttgccac tagttcagaa
    10141 aaaagaatgg tgggacagca tcgactcttg gagaaaagta tacagcacat ggtgtaaagc
    10201 ctacccagag cagcgtgaag aagtctttca attgtttgaa aaaaaggaag atttttggct
    10261 acgtcgaata gcaatcacgt tgcaattagg ttataaacag caaacagaca caggactatt
    10321 gatccgagca atagacgaag atcgtcaaac aaaggaattc tttatccaaa aagcaattgg
    10381 ttgggcatta cgcgattaca gtaagaccga tgccgattgg gtaaaaagac agctgacaac
    10441 agatttgagt aagttagctg ttcgagaggc aagtaagtat ttataagaaa gcgagacgac
    10501 aagagatgag acgcaaatta tttgcttttg atattgatgg aacgttgtta gattcaaaca
    10561 aacaagcttt agatagcacg agagaagcat tggaaaaatt acgcaaagcc ggtcatctgg
    10621 tcacgattgc tacagggcgg agccgatttc atgcgcaaga gatcattcgc gatcttgatt
    10681 ttacgaacta cattttatgt aatggagcgg ccggtttttt agaccatgaa caagtatata
    10741 agaatttact agatcgcgac cagttggatg cgttcgttca agaagcgcat catgagcaga
    10801 tcgatacagc ttttgtcagc ttagacaata tcaagcgttc ttcttccaat gatgtaacgg
    10861 tgatggaaga agcgatgcat tctttcggtg ctgtcttgcc tgaattagat cagttttttg
    10921 cagagaaaca agaaatatac caagcgttag catttttcga tcaaacatat gatgggaagt
    10981 tctcttcata tgataaaatt cgatttgtcc gctggcatga gaacagtgta gatgtcgtac
    11041 ctaaaaatgg ttctaaagca gcgacaattc tgcacttagc agaacaagta ggcatccctc
    11101 atgaagacat catcagcttt ggcgatggac aaaacgaccg tgaaatgttg cgcatgtcag
    11161 gaattgggat agctatgggg aatgctgtgc cagaagtaca agcagaagct aagatggtca
    11221 ctgatacaaa cgatcaggat ggaatttgga aggcattgaa agagttaagc gtgatttaaa
    11281 agaggttgtg aaaaagctgt tctgcatcaa gtaataagcc cagccaaaca aaaatagctc
    11341 ctcctatttt tcgtttttct gggcttatta ccgcagatgc aagcttttga acaccgttta
    11401 aggaagaggt cgtgacagaa gtggtcagtt tcaagaaata agaagggatt tacgaaaatt
    11461 gcttctcaaa tttttgtaaa attacggctt atttctgaag aagtagatga aacaacgttt
    11521 attcgggaaa tgggactgtg acaagatgtt tgtcatagtc ccattttttt attttatttt
    11581 tcaaaactaa gaaataccag gaaagagttc cttttgtttg accttttctt tcttattgaa
    11641 aacaactata atatagagtg atgttaaaag agaagaggaa tttagtgtgc gggtattgtt
    11701 tattggtgat gtagtaggtt caatgggacg agagatgatt actgaatatc ttcctagatt
    11761 gaagaagaag taccgtccgc aagtaacgat tgtaaatggt gagaatgccg cttctggacg
    11821 tggaatcaca gaaaaaatct ataaaaaatt tttgcaagac ggcgtagatg tcgtgacgat
    11881 ggggaatcat acgtgggaca accgcggaat ctttgagttt gtaaatgaag ctaaaaagat
    11941 ggttcgtccg gcaaattttc cggaagggac gcctggacaa gggatggtct ttgtcaaagt
    12001 gaaccaagtc gaactggcag tgatcaacat gcaggctcgt tcttttatgg tggacttgga
    12061 tgatcctttt cgtaaaatga atgagctggt agaagaagcc agaaaacgta cgccgatcat
    12121 ttttgtagat tttcatggag aaacaacaag tgaaaaacaa gcaatgggct ggtttttaga
    12181 tgggaaagtt tcagcagtgg tcggtacaca tacacacgtt cagacaaatg atgcgcgaat
    12241 tttgccaaga ggaacagcgt atctgacaga cgttgggatg acaggtccct atgatggtat
    12301 tttgggtatg cgcagagaac ctgtaatcga aaagtttctg accgcattac caaaacgttt
    12361 tgaagttgtg gaacaaggcc gatcgatttt aagcggctgt atcttggaat tggatgatac
    12421 gacaggtcat gcaaaagaaa tccagctgat tcagatcaac gatgatcgac cgttcatgga
    12481 gtagaaagag aggcagtttt ttgcagcagg agccggaaat tcaaaaagaa gtaaggaaac
    12541 tgacaaaact tttaagagag aatgaaacga tcattcgcta caaagaactt gaagaaaaga
    12601 tccagcaaaa ccagtattta gctgagttga gagaaaaaat caaacaagcg caaaaagatg
    12661 ctgttcattt tgcccactat gacaaaccag cagcagaaca agaagcaatc aaacaagcgg
    12721 atcaattcat gcaagaattt gatcagcatc cgcttgttgt agcttataga aaacagctgt
    12781 tggaagcaga tgatctgctt catcatttaa caactatgat acaagaagaa atcaatggac
    12841 aaatagagga ggaaaaacat gcctcaaaaa actaaaaata ctcctatgat ggaacaatat
    12901 ctaagcatca aggcccaata ccaagatgct tttttgttct atcgtttagg agacttttat
    12961 gaactatttt acgaagatgc gatcaaagcg tcacagcttt tggaattaac gttgacgagc
    13021 agaaatcgaa atgcagaaga acctattcct atgtgcggtg tacctcacca tgcagcacaa
    13081 ggatatatcg atgccttgat tgaaaaaggc tataaagtag cgatttgcga gcaggtagaa
    13141 gatccaaaga cgactaaagg gatggtcaag cgcgaagtga tccagctagt cactcctggt
    13201 acagtcatgg acagcaaagg attgtcggca aaagacaaca attttttaac agctgtagtt
    13261 agtcaaggaa atgaatttgg ctttgcttat gccgatttaa gtacgggaga attgaagacg
    13321 gctcggttgc atgatgaaga agctgtttta aatgaagcct ctgcattgca gacaaaagaa
    13381 ctcgtattag gcagtgagat cactgatacc cttcgtgaac agctaagcgg gcgtttaggg
    13441 ctagtctttt ctcaacaaaa tgagatggaa gaaaacgcgg aatttagctt tttgacaagc
    13501 gaactagtcg atccgctgga aaaagaagca accggcaaac tattgactta tttatcggtg
    13561 acacaaaagc ggagtttagc tcatatccaa aaagcagtag aataccaacc agatcatttt
    13621 ttgaaaatgg attattattc aaaattcaac ctagaattaa gccaatctat tcggactggg
    13681 aaaaagcacg gcactcttct atggttgtta gatgaaacaa aaacagcaat gggtggccgt
    13741 ttactaaaac aatggctaga ccgtccgctt atccaggcaa aacaaattaa agcaagacaa
    13801 gaaatggtcg cttctttgct agacagctat tttgaacgta tcgatttgca gtcagcatta
    13861 acgaaagtct atgatctgga acgcctagca ggtcgagtag ctttcggtaa tgtcaatggg
    13921 cgagacttga tccagctgaa aacttcttta gaacaagtgc caatgatcag agggttgatt
    13981 gaaggaatcg atcaaggtca atggcaaagt ctgctttcag aaatgcagcc gatggaccat
    14041 ctcgtccaat tgatcgacca agctatccaa gatgatccgc cgttacaaat aacggaagga
    14101 aacatcatca aagatggatt caatgagcag ctcgatatgt accgttctgc tatgagaaac
    14161 ggaaagaaat ggttggcaga acttgaagca aaagagcgtc aagaaacagg aatcaaaaac
    14221 ttaaaaatcg gatttaaccg cgttttcggt tattatatag aaatcacaaa agcaaatctt
    14281 ggaaatgcag aactggagaa gtatgaacga aaacaaactt tagcaaatgc agagcgtttc
    14341 atcacaccag aactaaaaga attggagaca caaatcctcg aagcagaaga aaaatctgtt
    14401 gatttagaat atcagttgtt tttagctgtg agagaagaag tcaaaaaggc tatccagccg
    14461 ctacaagtac ttgcaaaagc aatcagtgca gtagatgtgc tacaaagttt tgcaacgatc
    14521 agcgaacgct atcagtatgt tcggccagaa cttgtcagtg acaagcatca gcttctgatc
    14581 aaagacggac gacatcctgt agtagaaaaa gtattaggac atcaggaata tatccctaac
    14641 agcgtagaaa tggcagaaga cgaaatgatc ttgctgatta caggaccgaa tatgtctggt
    14701 aaaagtacgt atatgcgaca gttggcatta actgttctga tggcgcaaat gggctgtttc
    14761 gttccggcaa aacaagcagt tttacctatt tttgatcgga tttttacccg aattggtgca
    14821 agtgacgact taattgctgg acaaagtact tttatggtag agatgatgga agccaatcaa
    14881 gccctgcgtt atgctacgcc aaacagtttg attttgtttg acgaactagg gcgaggaaca
    14941 gcaacctatg acggaatggc gttggcccaa gcaatcatcg aatatatcca tcgtcatgta
    15001 caagcaaaaa cactcttttc tactcattat catgaactga cggtacttga gaaagaactt
    15061 ccgcagctta aaaatgtcca tgttggagcc gttgaaaaag acggagaagt tgtcttcttg
    15121 cataaaatga tggatggacc tgcggataaa agctacggca tacatgtggc aaagatcgca
    15181 ggattgcctt ccaatctact agaacgagca gcagatatcc tgcatacgct agaaaatggc
    15241 gataacggac accatactgt tccagctgaa gtagaagaga aaactaaaac agccaaacag
    15301 gagcaaataa aaccacctgc agaaaaagta aatcaagacc atatacaaga ccgtgtaaaa
    15361 gaagaaaacc aacagctttc tttattcagt gaattatccg aaaatgaaac agaagtcatt
    15421 gaatcactga agcaaataaa tttactagaa atgacgccaa tggatgcact aaacacatta
    15481 tatgaattac aaaaacaact gtaggaagaa ggcggatatc gatggccaaa atccaagaat
    15541 tatctgaacg tttagctaat cagatcgctg cgggagaagt agtggagcga ccagcttccg
    15601 ttgtgaaaga attagtggaa aatgcaattg acgccggcag tactcagatt gatatcttag
    15661 ttgaagaagc gggactgcgg acgatccaag tgattgataa cggagaaggc attttggcag
    15721 acgacgtaga gaatgccttc aagcggcatg cgacaagtaa gatccataat cgcgacgatc
    15781 tttttcgcat acgaacgctg ggctttcgag gggaagcttt gccaagtatt gcctctgttt
    15841 ctgaaatgac tctggaaacg gcgaatcagg aagaaaaaca aggcacctat ctgtcgctga
    15901 aaggcggaga agttattgag catcgaccag caccactgcg tcaaggaacg aagattaccg
    15961 tcagcaatct gtttttcaat acgccagccc gcttgaaata cgtgaaaaca ctgcacacag
    16021 aattagcgaa catcggggac atcgtcaatc gactggcatt gagtcatcca tcgattgctt
    16081 ttcgtttagt acatgatgga aacaaaatgc tggcaactgc tggaaatggc gatctgaaac
    16141 aaacaattgc gggaatctac ggattatcta ccgcaaaaaa aatgctgaaa atcgaaggaa
    16201 aagatctaga ttttgagtta tttggctatg tttcattgcc tgaggtgact cgtgccagta
    16261 ggaattactt atcgacgatc atcaatggac gttttatcaa gaacttttcc ttgaataaag
    16321 caatcgttgc aggttatggg tcaaaattaa tggtcggtcg tttccctatt gctgtattgg
    16381 aaatcaaaat ggacccgctt ttagtagatg taaatgttca tccgacgaag caggaagtcc
    16441 ggctgtcgaa ggaaaaagaa ctaacagagc taattagtaa cgccattcaa aaagcattgt
    16501 ctcaagaaaa tctgattcca agtgcagcgg ataatctacg gtttaagaaa aaagtacctg
    16561 atcagcctaa aacagaacaa atcgaaatcg atttgtccta cgcttttgaa gaagaaaaac
    16621 ctaaaaagcc aagtagtttg aattttgatc gagagacagg tacctttttc gtagaagaac
    16681 caaagaacac atttgttgac agaattgttg ataagtctgt ggaaaatgtt gaaaactatt
    16741 ctggtttttc tgaagaaaac accacttatc cagaaaaaac agcgaaaacg gtggataagt
    16801 ttggtgaaaa tgttgataac tttgtagaaa aaacagtgga aaaatctgaa aagaaagaat
    16861 ttcccattgt ggataacatg gagaaaacaa atgaagaaca agcagataaa tccgtagaag
    16921 aggatgaaaa gatttacgag gaagaattga cactccatcc tgaatttgat ttcactcaag
    16981 gaaacgacaa aacattagaa caagccatca agaaattaga aacggaaaaa ccagtacaac
    17041 gttttcttca gttggaatat tttggacaga tgcacggaac gtatcttttt gcccaaagta
    17101 gcgatgggtt gtacatcatt gatcagcatg ctgcccaaga gcgcattaag tatgaatatt
    17161 tccgggaaaa aattggggaa gtatctgatg atctgcaaga attgcttgtt ccatttgtgt
    17221 tagactatcc aaacagtgat gctttgaaat taaaagagca aaaacacttg ctagaagatg
    17281 cagggattta tttagaagaa tttggtcaaa acagctttat tgtacgggcc catcctactt
    17341 ggtatcccaa tggagaagaa gaatcgatta ttcgggagat gatcgatatg ctgctgacaa
    17401 ctggaagtgt gagtgtaaaa aaattccgtg aagcaacagc aatcatgatg agttgcaaac
    17461 gatcgattaa agccaaccat tatttgaatg aagcacaagc acgtgtgctg ctgaaggatc
    17521 tggcacgatg cgaaaatcct ttcaattgtc cccatggccg tccagtattg attcacttta
    17581 ccaattcgga tatggaaagg atgttcaaac gaatccaaga cccgcattaa gtcaaagaga
    17641 agagggaaaa catgaagatt gtattagcat cccaatcacc aagaagaaaa gagttattgg
    17701 ctcatatcgt ttcagaattc gagattgaac ctgctgatat cgatgaaacg ccatttccag
    17761 acgaacagcc cgtcgattat gttcgtagaa tggctgaaga aaaagcacga actgtctggg
    17821 aacaagatga aaaagaagac catttagtga ttgccagtga cacgactgtt gttctcaatc
    17881 aagagatcat gggtaaacca gaagatctgg ctgaggctga attcatgctg aaaaaactaa
    17941 gcggggaaac acatactgta tatacagctg ttgttttaaa aacaaaggaa cgagaagaac
    18001 ggatactagc agaagcacat gtcacttttt atcctttgac agacgaagag atcaaacgct
    18061 acttggagac gggggattat gcggataagg caggggcata cggcatccaa aatgctgcga
    18121 gtgtattcgt caaagaaatt tctggagatt attacagtat cgtaggattt ccgattggtg
    18181 ctgtcaatca ggcactgaaa acattcattt aaacaaaaaa gcggactatc tgattttcga
    18241 ttgaaaatcc atgatagccc gtttttttta ttgaaaagat tacttatcta ctctgactaa
    18301 gattttactt cagctttacc gactgcttcg cccgggcgaa caagtcctga ttggtcaaga
    18361 gaaagtcctg caactttttc attattggtc aaagcaacaa taatgtcagt tttcttgcca
    18421 gcttgttcta tttctggcaa atccatttca gcaatcaggg tctctggtgt gacaaggtat
    18481 ccttctttca cgaatacatt gaaaggaaca ccctttagtt caacagtatc taatcccatg
    18541 tgaacaagta cttccagccc ctcattagaa agaataccaa tggcatgttt tgtttcaaag
    18601 acactcgtca ctttaccgtt tactggtgca tagactttgc cgtttgacgg ttcgacagca
    18661 taaccgtcac ccatcatttt ttgtgagaaa actgggtcat ttacttcagt tatttccttg
    18721 atggtccctg cagcgactgc gaataatgtt tcttttgttg caactgccgt accagttgct
    18781 gaaacaggtt cgaaagactg tggcgttgct gttgcgccat ttgctgtttg aagttgtaca
    18841 tcagcagcat tttcaggaac agcaggatca atcttattca aaattccctg acgtctaaag
    18901 atgatcgtca agccaaatgg aatgatgaca gcaatgatca ttgcgatgat gaaaggtacc
    18961 catgtttctg cttggatagc tagtattcct ggcaaaccgc ctactccgat cgcgttagct
    19021 cgaacaccca tcaagttagc aaacatgcca gctaatccag agccgaccat tgcagcgacg
    19081 aaaggataga cgtatttcaa attgattccg aacatagcag gttcagttac gcctaagtaa
    19141 caagaaatca ttgctggaat cgagatttgt tcttcttttt tattcccacg atgtaagaag
    19201 atgatggcta agactgcaga gccttgggcg atatttgata aagcaatcat cggccataaa
    19261 ttcgttgatc cgaaatctgc aatcagctgc atatcgatgg cattggtcat atggtggaga
    19321 ccagtaatca ctaaaggagc atacaggaat ccgaatacgg cactaaacaa ccagctgata
    19381 gaagacgtca atccagtatt tacgatattt gagatccagc ttccgactgt ccagccgata
    19441 ggacctaaaa taacgtgtgc agctaaaaca gttggtaaca atgaaaacaa tggaacaaaa
    19501 atcatcgaga tcgactgagg gatatatttg cggaagaaga tttctagata cgctaacata
    19561 aaccctgcca gcatggctgg aataacttgc gcttgataac caatcatctg gacttgagca
    19621 aaaccaaaat cccaaaatgg aatatctgct gcggctgtgg aagctacact ataagcattt
    19681 aataattgcg gagaaactaa cgtaattcct aaaacgattc ctaatatttg tgtagtaccc
    19741 atcttttttg caatactcca agtgatacct actggaagaa aatggaaaat tgcttcaccg
    19801 ggtaaccaca aaaaggcatt gacaccattc caaaacttcg agtgttcaac gattgttcca
    19861 ccaagacttt caaactggat accttcaagt acatttctga atccaaggat caatccaccg
    19921 acaatgattg caggaatcag tggtgtaaag atttctgcta aaacggcaat agcacgttga
    19981 acgggatgaa ggttttgttt tgcagctgct tttccttgtt ctttggaaac cccttcaact
    20041 ccagatactg caacgaagtc gttataaaaa gtagaaacat cgtttcctat gatgacttgg
    20101 aactgtcctg cattcgtgaa catccctttt acgctaggta tatcttcgat cgctttttcg
    20161 tccgcttttc ctggatcatt caggacaaaa cgcatacgag tcgcacaatg actaactgca
    20221 gcaatatttt cttttccgcc gatatcatgt aacagcttct cggcatctgc ttgatatttt
    20281 gccatatcaa taaacccctt tctcttaact tgtctatgca agttgtttct tcatttacta
    20341 tacttttttt cggagagaat tgcaagcgtt ttattaaaaa gtacgagaga aaatgatatc
    20401 gttttaaaaa gcttaaaata agcttttctt aaaaatagga acattgaaat acaaaaagag
    20461 ctgtagtata gtgaagaaat gatggaagaa cgagtgaggg aaaaaacatg aataagttca
    20521 atgaaatttt tttagatttg gaacagaaaa ttttagcgaa agaatatcct ccgcattcgc
    20581 ttttacccag tgagaaccaa ttgatcaaaa tttatggtgt ttcacgagaa acaattagaa
    20641 aagcgctgaa tcttttgact acggaaggtt atattcaaaa aaaacaagga aaaggttcga
    20701 tcgtactgga tcggaatcgc ttcgactttc ctatttctgg attgactagc tataaagagc
    20761 tacaggagac ccaacgtatt ccaagtgaaa cgatcatcca tacgttagaa gaaacagaag
    20821 tgacaaaagc attgaacgag atcactggct gggagatcgg tgcgcctgtt tggcatttga
    20881 tacgcgaaag aaaaatcaat ggcgaagtag ttatattaga caccgattat ctattaaagg
    20941 aaatcgttcc tattttaact ccagcacaag cacaaggatc gatctatgaa tattttgaaa
    21001 atgagctttc tttgacgatt gcttacgcgc aaaaggagat cacagtagaa gaagtaacgg
    21061 aaatcatgaa aacagcgatg gatcttcacg atgacactta tgcagtagta gtcagaagcc
    21121 tcgttcatct ggaagatacc cgctgtttcg agtattctga gtcggttcac cgattagata
    21181 aattccgttt cgttgagttt gcccgacgcc gaaaagtgta aatagttcaa aagaaagcaa
    21241 attgttggca tctgtccact ttggcgctat gataggatca caaagggggc atttttaatg
    21301 gataaaccaa taagaccaga aaaagaagaa ctgaaagaaa agttatcacc catggcttat
    21361 gcagtcacac aagaaaatgc aacagaacga ccatttagcg ggaaatatga tgatttttac
    21421 gaaaagggga tctatgtaga tatcgtcagc ggtgaaccgt tattcagctc tgcagaaaaa
    21481 tatgatgcag gttgtggctg gccatcattc agcaaaccaa taacgaaaag acaagtgcgc
    21541 gaaaaagccg atttttctca tggcatgcat cgaatagaag ttcgaagcaa agaagcagat
    21601 tctcatctcg gacatgtgtt taccgatgga ccagtagatc aaggtggtct acgttactgc
    21661 atcaattctg cctcactgaa attcattcca tatgaaaaga tggatgaatt aggttatggg
    21721 gaattcaaat cacttgtaga ataatggaaa tacacaaaga gcggtacaat acggggattt
    21781 actcgatcgt ccgctctttt tttattcaag cgattgctat ttgaaagaaa cgctccctag
    21841 actccttttt tcacaaggtg aaatgtgcta caataaaggg aacgtatgtg tgcaaaagaa
    21901 agaggactag aatatgtatg aatatctgac aggtgtggtt acctttatca acccctatta
    21961 catagtagta gaaaccaatg gtatcggcta ccagatcgca ctaggaaatc cttaccgcta
    22021 cagtagcaaa ctaaatcaaa aaataaaaat ttatgtccat caggtaatac gtgaagatgc
    22081 gcatcttctg tatggatttg attcgctaga agaaaagcaa ctgtttttgc gattagtaag
    22141 tgtttcagga atcggtccga aaagtgccct tgcaatcatg gcaagcgaag accactccgg
    22201 tttgattcaa gcagtagaat ccagtgatgt cacttatttg acgaaatttc caggagtagg
    22261 gaaaaagaca gcacaacaaa tgatcttgga tctaaaagga aaattcggtg agctgagtat
    22321 cgatacacct tttagtctat ttgatgaagc aaacacaaaa gacgctacag ctctatctga
    22381 agcaatggaa gctctctcgg ctcttggtta cagtgatcga gaaatcaaac gtgtagaaaa
    22441 acaattaaaa gaagtagata atcagacaac ggatgagtat ctacgccaag cactcaagct
    22501 aatgatgaaa aaataaggag gagaccaaat gacagatgaa tacttgttat caccagaatc
    22561 tggcgaaaat gaactgagtt tggaaaaatc tcttcgacca cagctgcttt ctcaatatat
    22621 cggacagaga aaagtcaaac aagaacttca gatttacatc gaagctgcca gaaatcggga
    22681 agaaccttta gatcatgttc tgttgtacgg gcctcctgga ttaggaaaaa cgacgatggc
    22741 catggtcatt gctaatgaga tgaccgtaaa tattcgtacg acgagtggcc ctgctatcga
    22801 acgtccgggt gacttagtag caatcttgaa tgaactggaa gcaggggatg tcctgtttat
    22861 cgatgagatc catcgattac ctagagtggt agaagagatg ctttattcag ctatggaaga
    22921 tttctatgta gatatcatgg taggacaagg gccaaccgca catccggttc atttccctct
    22981 tcctcccttt actttgattg gcgcgacgac acgggcaggt atgctttctg cacctttacg
    23041 agatcgattt ggcatcattt ctcatatgga atattatgac gaacaggatc tgcaggagat
    23101 cgttttacgt tcttcggata tttttcaaac agagatcatt gaagaagggg cattggaaat
    23161 tgctcgccga tcacgaggaa cgcctagaat cgctaatcgc ttgctcaaac gggttcgtga
    23221 ttttgcgcaa gtccaaggag acgggaaaat cgatcgtcct atagcggatc aggcacttac
    23281 tttgctacag gtcgatcagg ctggattgga ttatgttgac cagaaattgt tgcgaacaat
    23341 gatcgagtta tatggcggcg gaccagtagg gttaagtacg ctttcagtaa acataggaga
    23401 agagcgtgag acggtagaag atatgtatga accgtatctg atccaaaaag gatttttaaa
    23461 gcgtacacct agagggcgaa ttgctactgc ttatgcctat gaacattttg gttacgacta
    23521 tcatatatga tttttgcttt tagattggag gaaaatgaat gagtgataca acacgtacga
    23581 aacaattgat caaaaacgta ttgattgatt tgtgccaaac gcaatctttt cgaaaaatca
    23641 gcgtccaaaa aatcgcaaac gaagcaaaaa tcaatcgaca aacattttat tatcatttta
    23701 cagataaata cgatttgctt cgttgggtct attgtgagga tgcattgcat tatttagatc
    23761 cacaatgtct ttcacttgat aattgggaag agcaagcatt gaaaatgctg aaagcaattg
    23821 ctgagaagaa agaattttat gcaaatactg tgctttctga cccagaaatt ttacaaaaag
    23881 agttttctca actgatccaa aaatcattta tcaagatttt tgaacaaatg gatgaagaaa
    23941 gacaactttc tgcaagagac aaactatttt atgctcgttt tttttcttac ggttgcagtg
    24001 gtgtattagt caattggatc acgaatgaat atgaagaaac accgatggaa atcgctatgc
    24061 agcttttccg tttagccaag gatacggaat tatattctta tcgtctttat gcaaaagaag
    24121 agttggaaga acagtaatag aaaaattttt tgttggaaaa gcaataatcg ctaaaaggag
    24181 atggggaaat gacaaaaatt ttatttgttt gtttgggaaa tatttgccgt tcaccgatgg
    24241 cagaaggctt gctgagaaaa cgaatagcat tagaaggaag ggaagcagaa ttttttgtgg
    24301 attctgctgc tacaagcacg tatgaagttg gaaagacacc gcaccctgga acgaaaaaaa
    24361 tattaaacca agaaaaagta gatatgacga atatggttgc tcggcaaatc acaccccacg
    24421 attttgaaac ttttgattgg atcattggca tggatcaaga aaatgtagaa gaactgaaac
    24481 gaagagcacc acgctcagcg caggggaaaa tccatttgtt tttaagtagc attcctggaa
    24541 aagaaaaaga aaatgttccc gacccatact ataccaataa ctttgatgag acatatcact
    24601 tgatcacaga aggcctgaat cattggtgga atatttggat tgatgaaaat aaaataacaa
    24661 acaatttgta aagttaacgc tttcttttta aggcgatgtt agagcatttc cttgtttttt
    24721 tgtaaaatgt taggtaaaat aaaattaatt aagtaacact ttcttagcta aaggaggagt
    24781 gcattcgatg aacgaagaga cagtatcgcc aatgaagaag cttacgaagt tagttactca
    24841 tacttcgtat caactttttg attcttttgt aaatgacact gaagaacatg ctaagaatgt
    24901 acgattgcaa ggaaagttta ttcttgagct gcaaaaagct gctactgaac atagtctagt
    24961 tatcttacaa gtaagagcaa atcgtttgag tagcaagtat gaaacgatct caggctgggt
    25021 ggcgacaaaa acggtcgaaa aagaccgagt gatgatccgc ctacaaaacg aagagcagca
    25081 gatccggatg gttcctgtag aacaaattga aaaaataaca atcctttcac cagcagggga
    25141 acaggaacgg ttatcaaaat aatttatacc agatagaaaa gcacgtatga gcttgtacca
    25201 tcaatcatta ctaatcaaaa ataaccgaat gctttaagct gatatcagta aaacggaacg
    25261 ttttaggatg agtatcatag catgacagca ttcggttgat tagttgaatc gattggatgg
    25321 tacaagtttt tttgataaaa aaaggtcctg ctagccgggg agctagcagg ataaggagtt
    25381 aaaaatgaaa aagtgttgtt agggttgttt gttggtatgc ttatataata gtcattagtt
    25441 gtgaatattt tgtgacgaaa gttttcgaaa g
//
LOCUS       DACDVT010000034        24941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDVT010000034 DACDVT010000000
VERSION     DACDVT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.034"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(9..2153)
                     /locus_tag="IWL52_001447"
     CDS             complement(9..2153)
                     /locus_tag="IWL52_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9778018.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFNHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2510..3883)
                     /locus_tag="IWL52_001448"
     CDS             complement(2510..3883)
                     /locus_tag="IWL52_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9778019.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3883..4278)
                     /gene="rbsD"
                     /locus_tag="IWL52_001449"
     CDS             complement(3883..4278)
                     /gene="rbsD"
                     /locus_tag="IWL52_001449"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9778020.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4306..5223)
                     /gene="rbsK"
                     /locus_tag="IWL52_001450"
     CDS             complement(4306..5223)
                     /gene="rbsK"
                     /locus_tag="IWL52_001450"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9778021.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5331..6344)
                     /locus_tag="IWL52_001451"
     CDS             complement(5331..6344)
                     /locus_tag="IWL52_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9778022.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6425..7024)
                     /locus_tag="IWL52_001452"
     CDS             complement(6425..7024)
                     /locus_tag="IWL52_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9778023.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7210..7911
                     /locus_tag="IWL52_001453"
     CDS             7210..7911
                     /locus_tag="IWL52_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778024.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7921..10632
                     /locus_tag="IWL52_001454"
     CDS             7921..10632
                     /locus_tag="IWL52_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9778025.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11083..12711
                     /gene="treC"
                     /locus_tag="IWL52_001455"
     CDS             11083..12711
                     /gene="treC"
                     /locus_tag="IWL52_001455"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9778026.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12771..13952)
                     /locus_tag="IWL52_001456"
     CDS             complement(12771..13952)
                     /locus_tag="IWL52_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9778027.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDYTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14204..15727
                     /locus_tag="IWL52_001457"
     CDS             14204..15727
                     /locus_tag="IWL52_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9778028.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15796..16524)
                     /locus_tag="IWL52_001458"
     CDS             complement(15796..16524)
                     /locus_tag="IWL52_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9778029.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16537..17817)
                     /locus_tag="IWL52_001459"
     CDS             complement(16537..17817)
                     /locus_tag="IWL52_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9778030.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17958..18560)
                     /locus_tag="IWL52_001460"
     CDS             complement(17958..18560)
                     /locus_tag="IWL52_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9778031.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18805..19011
                     /locus_tag="IWL52_001461"
     CDS             18805..19011
                     /locus_tag="IWL52_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778032.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19323..20042
                     /locus_tag="IWL52_001462"
     CDS             19323..20042
                     /locus_tag="IWL52_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9778033.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20123..20806)
                     /locus_tag="IWL52_001463"
     CDS             complement(20123..20806)
                     /locus_tag="IWL52_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9778034.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21024..23210
                     /locus_tag="IWL52_001464"
     CDS             21024..23210
                     /locus_tag="IWL52_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9778035.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23556..23966
                     /locus_tag="IWL52_001465"
     CDS             23556..23966
                     /locus_tag="IWL52_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9778036.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            23980..24336
                     /locus_tag="IWL52_001466"
     CDS             23980..24336
                     /locus_tag="IWL52_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9778037.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8362 a         4429 c         4860 g         7290 t
ORIGIN      
        1 aatttattct atttctttaa aggcagatca agccagaatg atttgtctaa acttaacaag
       61 gttagtacag gaacagttgc agctttaact gtacgagtag ttgtttctgg tatctgtctt
      121 gccgtaaaat tgttccacaa atcccagatt gaagcgacac ggaagatgga agaagcgttg
      181 ttgacgcgtt ttatcgttga aaattctaat cctgtgattg gcacgccatc tgttgtccat
      241 ttgattggca catcgaatcg cttagacata ttgaggattt cagcgtttgc ttcttcagtt
      301 gttgctgctt cttttttcgg ttcatcatat tttgtcaggt cgtacgcttc gatgatcgcg
      361 ttcaattttt gtgaatattg gcgatctgta gcataacgtc cttccaaata tttggttgct
      421 tctttgtagt cttttgtttc cgacttccaa gttggtttgt agaaatcagg attgccagtc
      481 agtccatttt tgatcaagtc ggcatagtct tccaaacttt ccttgtaaga aggatattta
      541 cggaattgtg cgcggatttg gtagctttgt ccggaactat cttgttcaag tgtattgaag
      601 gtcacgctgc ttcctttata tgatcctttg ataccaaaaa ggttatgatt tggttcgctt
      661 gataataagc tgtttccaga tccactttca aggatagctt gagccatcat gactgatgcg
      721 tagattcctt ctttttgccc gatttcctgt gcatcatctg caatcttttt caagaactga
      781 tcagttgttt ggttttgact gaaatggaac gagccatcta atccatctaa aatagatgag
      841 ccgcctgtca gcgaacctga agcagcaggt aaacctgcta agtttactcg gacagcaaaa
      901 tcaggcgtga aatctgaaat agaagttact cgaactttat ctcctggttg aggcgcatgg
      961 ataaattgtc cgttcccaat atagatacct acatggtgag taacaccgga agaaggtccc
     1021 cagaagacga ggtcgccagg ttgggcctga cttactggaa tctgcgtacc tgcatattgc
     1081 tgttcgcctg tccagccacc gatataatga ccggttgctt tcatatatac gtaataagtc
     1141 aaaccagaac agtcaaaagt atttggacct tttgctcccc atacatatgg ttttccaaga
     1201 tgattgaatg cttctcgaac gattgcttgt tggacattcg ttgtatttgt aggcaataca
     1261 gcatcaattg gtgattttac aacaaaatca gaagaagcag aagatggatt ctctataccg
     1321 ctttgatttg ctgtatttgg acgagatgaa gcattgttcg ttccgccatt cgatgaacca
     1381 ttgtttggat ggtttggttc tctctgagta tttccattgt tattcgatcc agatgggttt
     1441 gtttgattcg aagaattccc gctgttcgaa ttattgttat tattgtttga attatttgaa
     1501 ccattattat tgttgttatt acccacatct ggtttgtttg gcgtttccgg atctggtgta
     1561 ctagggactt cttcactagg atcagttggt ttctctgttg aagtatccgg tccagcttta
     1621 aattctgcag taatagataa attgttcgta cctactgtaa aggtctttgc atcataattt
     1681 acttctttgg caccattaac agcgactgaa gcaatttgtt caccttcagc tggcgtaata
     1741 gtgtaagtta ccttcgtttc cgctaataaa ccttctaccg ttccactttc cgtaatatcc
     1801 aaaggttgct gttcagggga atccgctgtc gaagcagtca gttggatctt accaatcaaa
     1861 gaaggagcaa ttgtaaggga atatgtctcg gctggctttt cattttctga tggatcagcc
     1921 ggatcaattt cagttggctc ggtcgatcct gttgttgagt tagttgttcc agttgggtct
     1981 tctgtaaaag aatccgttgt ttcactagta gctgagctgg atgtagtcgt atctgttgtg
     2041 actgtttgat cagctatttg attatctacg gcaaatacgg tattggtcga caaaacagac
     2101 ggagcaatca gccaagtagc agctagcaaa cgtatcgttt ttttattttt cataataaac
     2161 tagtcctttt cctttcattg attttctgtc ttattcagca ttcattagac attttttgga
     2221 acagataatc gtgtcgaaaa gctttttctt ttcataaatc tgtctgtgta ttctaaataa
     2281 aacaatttgt aaataagcaa cgagcattca ttcgtttttc tttgacgttc attttattgc
     2341 atagaccacc tttgtttggg gataaacaag tggggataaa aatggttatg catatgtaaa
     2401 ttataccaaa gtttggaaag tatcacaaaa atttataaaa ataatgatcc taaaaagaaa
     2461 aagactgaaa tatgtgataa acatctcata cttcagtctt tttcattaac tattcataag
     2521 acattaggtc ttcatcaaac ccgctaacga gagtagtgat cattgctacg actagactaa
     2581 cagccagtcc tgctaagtac cagaaaaaag taccggaaat gaatgcaggc aatgtggtca
     2641 gactaccaaa tacaaaagcg atcgctttca cttgggcaaa tcccattaca gctccaccag
     2701 cagctgcacc aatgcaagca gcaacaaatg gttttttcaa tgggatcgca attccgtaaa
     2761 cagttggttc ggtcactccc gcaagtaaaa tcgaagcaaa agcagaaccg gagatcgaac
     2821 gggtttttga acgtttactt cgcagaaata cacctaaagc tgcaccagcc attccaaaat
     2881 tggctgctcc tgtcaacgga acgattcggt cgaacccata agcagagata ttactgatca
     2941 tgacagggtt tacagcccat tggataccaa atattaccaa aatattccaa ccaccagcga
     3001 tgattgcgcc actgatcggt ccgttaatat ccatcatcca agtaattgcg gcagctaaac
     3061 cttcgccaag gtaaacaccc gcaggcccga ataccattaa cgtcaaagga accgtgataa
     3121 ctaatgaaag taaaggtaca aatactaatt gcatattttc gcgaatatat tttttgagga
     3181 atttttcaac aaaagaataa aagaaaatac ctaaaatcgc cggcatgaca gtagacgtat
     3241 actgcatcag tactacagga atccccagaa aatcagtagc tgctttgcca ctgtctgaaa
     3301 gtaaagaggt cagattcggt tcaatcagtg ctgcagcaat gacagccgca atatatccat
     3361 tcactttaaa cgttttggca gcagaaaaag caagaaagat tggcagaaaa taaaagacgg
     3421 cattcgacgc agcagacaaa atatgataag ttccgcttgt ttcactaagc cagccaatcg
     3481 tagtcgctaa aacaaccaga ccttttaata taccagaacc ggcaaacagc ggaatgatcg
     3541 gcgtaaagat ttccgatatc aaaccaaata gtttttcgat cggatttagc ttttctttcg
     3601 gagctgaatg atcgatttga cgcgtcaatt ccttgtaagc aggagccaat ccctgatcca
     3661 tgattatttc tgcatatcta tcgagtaaaa tgaaatactt aatggaaggg atttgctgtt
     3721 ttaatttttc tgcattggct actggctgtt ttttcaattt gatacgaagc cgagtcgcac
     3781 atggaaggac agaatcaata ttgtcttttc ctcccaactg ttcaatcagc atatgaatct
     3841 gctcggacag taccatctgt tcaggattca ttgtcgtttc cattagaaga ccactccact
     3901 cactaatata atattggcgt aaggtgtcat ttcgcctgta cgaataaatg catgacagtt
     3961 attcaactct tttttcattt cttcatgtgg gataaagcta atcggtgtat ctggtaacaa
     4021 taatttgatt ttttccaata tttccgggtt cattgtcttt atttcttccg ccaattcaat
     4081 cgattgtacc tcaagttctt ctaaaacgtt ttctaaaaca gataaaaaac ttggaagacc
     4141 gtttgagaca gccaaatcta ttttttcagt accaaagggg acaggcatgc ctgcatcccc
     4201 gatacttaat ttatcaaagt gccccatttg tgcgattact cgtgaaatat ctgaattgat
     4261 tacttttcct tttttcatgt ttgtttcttc ctctcattgt taattttaga ctgctgattg
     4321 ttctaaagct tcgcggtaag ggatagaagg ttgcgcacca taagactgca cagcaacaga
     4381 agaggctaga tttccataaa ggatcgcttc tttcaagtta ctaaggtctg gctgaagtgt
     4441 gctgcttaat gcgccaataa atgtatcacc tgcggctgtt gtgtcaacgg catccacttt
     4501 aaaagcaggg acgataccgt gttctttttc tgtatgatag aaagcgccgg cactgcctag
     4561 tgtaatgatt actgttccaa tacctaattc atggagtttt tcggctgctg cgaccaaact
     4621 gttatgatct gtcactctga ttcctgtaat gatttctgtt tctgtttcat ttggtataat
     4681 gatatctgtc ttttcaagta gttcagtagg aatcgtttca cgagctggtg caggattcaa
     4741 gatcgttgtt tttcctgcgt cttttgcaat agaaaaagct aggatcgtcg catctaagct
     4801 actttcaaac tgagcaataa taaagtcact gtttgtaatc agttgcttgt tttttaggac
     4861 ttcttgttca tggaaagcat tatttgcccc tgcatggatc aagatactat tttctcctgc
     4921 tgcgtctacc ataatcattg cttgtccggt tgtggcgcct tggatttcag ctatcccaga
     4981 tgtatcgatg ttttcttttg tcagtagatc taataactgt tgtccttcgc tgtcagcccc
     5041 aacgcctcca ataaaagatg tccgtgcgcc tgaacgtttg gctgccactg cttggttggc
     5101 acctttacct ccgccagcgt gaaaaatctc atgcgcatgc atcgtttcgc ctggcttggg
     5161 catgttggtt aaacgaaggg tagtgtccat attaatactt ccaattactg taaccgtgtt
     5221 cattttacaa acctccaact atctcattta ttttgatggg aaaattattt tttaaaacgt
     5281 tttagtaaaa cgttttaata gttcgataaa aatatagcac accttgaaag ctatttcaag
     5341 ggtgcagttg aaaatctttt ttctaatcgt acaggtaatc ttttttcttc ccattctttt
     5401 tctgggaatt ggatccgatc taacaataac ttggcagatg tttgtcccaa ttcgaagatt
     5461 ggttgtgcga tcgttgtcag tttcggcttg atatactcgc acatatctat attatcatag
     5521 ccaataatgc tgtagtcttc agggatgctt tttccagctt cctctaatcc gcgatataat
     5581 ccaaaagcta attcatcatt taaagcaaac acaccagttg cagcagaatc aagaagttct
     5641 gctgtgatct ggtagccacc ttgttttgag aactgtgtag gtaagaggat cagttggtcg
     5701 tgagggattt ggtaaacatc aagagcagat ttaaacccct caatccgagc gtggacattt
     5761 tccggtggat tttcaggata gacaagggcg atcgtttgat gtcctagact aagcaagtgc
     5821 atgcccgcta ggtaaccacc gcgaaaatcg tctgttcgta cggcatcact aaatccttca
     5881 gattttttct gatccaaaac aatgaagggc cggccttgtt ttttcaaatt ttcgttgatt
     5941 gcatctgtag agacagcaga agtcgcgata atgaatccat ccacacctcg acgtgtgagc
     6001 tcggctagat attcgatttc tttttgatga tcagaatcag cattacataa tatcgtgacg
     6061 aaattctgct tatacaagat atcctcaatt cctcgcatca gtgtactgaa gaatggattc
     6121 gtgatatcag gaacgagtac accaattgtt ttcgtttcct tggtaatcat ttgtctagca
     6181 aaatagtcag gctgatagcc taattcttct ttggcagcta aaactttctg aacagttttc
     6241 ggactgaatt tctccgtatt tccatttaat atcaaggaga cagttgtgat tgaaacgccg
     6301 gaaagtttag cgacttcttt gattgtgatc ttttttttca ccatgttcat actcctatct
     6361 aaatcctgtg gttattttcc catgaaatca aaaagaagta aatgaaaatc acgtgtgcca
     6421 gtacttattt gctttgttgg atagcttcga ttatttgttt cgttgagcgt actcgtgcag
     6481 ttttcggaaa tatctttttt atgaacaatt gatgggattc tttgtcacga tcactgcaag
     6541 catcttcagc aagcacgata tggtacccgt gttgaaaggc atctaatgca gtagcatata
     6601 caccgtttgt tgtcgctaca ccgcttaaaa tgatagtttc gattccacgc cggcgaagtt
     6661 gaagatcaag agaagtacca aaaaaagcac ttggattgta ttttgtgata gtcaacatat
     6721 tatctgtatc tttcaaagaa tccttcaaaa gtaaatcagt gaattctgga ggtacagaag
     6781 cagtcattcc acccatatca gtttgttgac tcaaataagg gaaagtggag atgtctacgt
     6841 tcactaaaac tttcaaggca gcggtacgtt tcaatgttct tactaacaaa tcattgtttg
     6901 tcagaataga tgctgcagtg tgtggagaga gtgtaccgtt atttacgatc tctttttgca
     6961 tgtcgatcgc gacaaaagca gtttttgata aatcaaaaat catattttcc tttaaatcat
     7021 tcatgatgtt ccttctttca aataacaatg ttttttatgt aagtaaatca taaatcgttt
     7081 atttgtcaac taaattaaag gattcctttc tttagtctag agaaattcag tggtaaaata
     7141 aataagtgtg agaaaacgca ttcgatgcat agatatagcg agaaatatcg tgaaaatgga
     7201 ggaatcagag tgaagtatgt cgaagtaaaa aatgaataca aacgatacaa aatgggagaa
     7261 acgaccatta cagcaaatga cgatatttct ttttcaattg aaaaaggcga attagtgatt
     7321 atacttggtc caagtggagc aggaaagtca acgattctta atattttagg tgggatggat
     7381 acacctgatg aaggtcagat cattattgat gacacagata ttgcgcaatt ttcagataaa
     7441 cagcttactg cttaccgacg aacagatgta gggttcgttt tccaattcta caatctcgtt
     7501 cctaatctaa cagcaaaaga aaatgtggaa ttggcaacgg aagtttctcc tgatgcgctt
     7561 gatcctgtag aggtattgag gcaagtgggc ctagctcatc gattgaataa ctttccgtcc
     7621 caattatcag gaggcgaaca gcagagggtt tcaattgctc gtgcactagc taaaaatccc
     7681 aaacttcttt tatgtgatga accgacaggc gcgctggatt ttgaaacggg caagcaagta
     7741 ttgaaattat tgcaaaacgc tagcagacaa cacggcaata ccgttttgat cattacacat
     7801 aattctgctt tagcgcccat cgctgataga gtcattcaca ttaatgatgc taaagtaagg
     7861 tctgttgaat taaatgatca tccttcatcc attgatgaga ttgtatggta gggggatttt
     7921 atgaaaacaa gaacatatct taaagcaggt tttcgcgaaa ttcggcaatc taaaggacga
     7981 ttcattgcaa tcatccttat tgtcatgcta ggaacacttt tatttgtagg tgtcaaaact
     8041 gctggacctg tcatgcaaaa aacgatggat cactatgttg gtacagcagg actttctgat
     8101 ttgcaaatcg tttctactgg tggcttgaca caaaaagata ttgctcaagc agaaaagatc
     8161 ccagatgcaa aagtagaaac aggaaaacag atctattatt ccaatactgg caaaaacgaa
     8221 gtgattcaaa tattttctta taacaaaaat aacaagcaga acaagctaca attaactgat
     8281 ggaaaactcc cggaaaaacc aaaccagctt gtattggatg aaaaagcgga agatgaaggg
     8341 tataaaatcg gtgatattta tcagattgat tcagatgaat tgaaagaaaa agagtatacc
     8401 atcacgggct ttgtccgttc tccattgttc atcaacaatt tggagagagg atacgcgaac
     8461 gtcggaaatg gtagcgtcga ttattttgtc tatttacctg aatccgtttt taaaacagat
     8521 atttattctg tcttatatct tgattttaca aacgtaaaga atttgaatac gtacagcaaa
     8581 gcttacaaag ataaaatgga gcaaaatcaa gaaaaggcag aaaagtattt acaagatcga
     8641 cccgaagaac gcttagaaga gctgaaaaaa tcggccaacg aagaattaga accggcacag
     8701 caaaaaatag cggatggcaa agcacaaaca acgcaagcaa gaacacggtt agaagatgct
     8761 aaaaaacagt tagcgcaaca gtcagcaact atccagcagc ttcctgaagc gcaacgaaca
     8821 atcgctgttc aaacattatc tcaacaagaa gctcaacttg ctgaacaaga aaggcaatta
     8881 gttgagaaag aacgtgaact ggcctctgca gaggaagaat tgaatcgaaa tgcagaaaaa
     8941 attgaaaatt taactaaacc atcataccta tacaatgaac gttcagaaaa tgtcggtttt
     9001 caagaatttg gtgatctgtc tgatcgtatc gctgcgattg ccaatgtttt tcctgttttc
     9061 tttttcttta ttgccgcatt gatcaccttt acgacaatga cacgtatggt agaagaaaac
     9121 cgcagggaaa tcgggacatt aaaggcgtta ggttatacga aagttgaaat cgcaaaaaaa
     9181 tatgcaatct atgcaagtct agcctcaggg ataggtattg ttttaggaac gatattagga
     9241 acgaatcttt tgccaaggat catttttgag ctgtctaacg agcggtatga tattgggagt
     9301 gcgcttattt tttatgactg gccgccgatt ttacaagcag ctgccgcatt cttcattgcc
     9361 gcatttggcg ctgcaatgat cgttttattc aaagatttaa gagaacggcc tgctgcattg
     9421 cttcaaccga aagcaccgaa gccagggaaa cgcatttttc ttgaatacat cactccgctg
     9481 tggtcacgcc ttagctttaa tcaaaaaatc agttaccgaa atctcttccg atataagtca
     9541 agaatgctta tggcgattat cggaattgct ggatgtgccg ggctgatggt tgcaggcgtg
     9601 ggattgaaag attctctaag ttctgtttca gccaaacaat ttggaacgat cattgactat
     9661 caagcaatcg ttacattgga ttcatccacg gaagatagtc gtcaaaaagc agcggatatg
     9721 ctgcagaagc aagaaaaagt aaatgagatc ctagctattc atactcaaac cgtagaattg
     9781 agaaaaaaag gacatgcttc gcaaagtctg acagtgatcg taccaagtaa aacagctgat
     9841 ttggatcgtt ttatccattt aactgatctt gaagagaacc cacgctcctt gcctaaaaca
     9901 ggaatcgcta ttacgcaaaa agtagcagaa ctatttgact tatctacagg agatcatatt
     9961 tcgctttatg atgaagatca aaaagaatgg aaagtcgaag taaaagaaat cgtgaaaaac
    10021 tatctaggta gctttattta tatgtcccct gaatattatg cttcagttac tgatcaatcg
    10081 atgatggata atgcattttt gctgcgtaca gataagatga caacaaaaga agaagaaatg
    10141 ctttccgaga aactgcttgc cacagacgct gtgacaaata cgtctttcat gtccaaacaa
    10201 atcgaaacgc aagaagaatc aatggcgaac ttagactcgg ttgtcttaat ctttgtcgta
    10261 ttatcaggac tgctggcttt tatcgtcttg tataatctaa ctaatatcaa tatttccgag
    10321 cgtgtacgcg aattatctac aataaaagtc cttggatttt ttgataaaga agtcacgatg
    10381 tacattgttc gagaaaatat catctttact cttttaggaa tcatcggagg atttggtgta
    10441 ggatatatat tgactgactt catcttgcaa caagcgtcta tggagaatgt catctttcca
    10501 ttagtgatca catggtgggc ttatgtctta tcagcaggat taactatcgt atttacggtt
    10561 attgtgatga ttgttaccca tttcaaatta aagcatatcg atatgattga tgcgttgaaa
    10621 tcgaatgaat aaaaacagag gttgtgaaat cagcggtttg acctgagtat tagagaaaca
    10681 aacggcaaaa tagtttttca tattttgccg ttttttgagc taataccgaa ggacagctga
    10741 ttgaacacca tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact
    10801 caaataaaat tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc
    10861 ttttgaacac catttataga agaaacaaga atattttgct attttaaaca agaggataga
    10921 caaatacatg tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag
    10981 ataaatttta caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa
    11041 gttgtggtag gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa
    11101 gtcatttacc aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat
    11161 ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg
    11221 ctaaacccta tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca
    11281 gccatcgatc ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag
    11341 aaataccgga tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa
    11401 tggttcaaaa aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa
    11461 gagccaacgg attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg
    11521 gggaaatatt atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa
    11581 gcagtaagag aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga
    11641 ttccgtttcg atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa
    11701 aatgatggca aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg
    11761 aaccaagcaa cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg
    11821 acgatcgaaa attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt
    11881 aatttccatc atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt
    11941 gattttgcag aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac
    12001 ggctggaatg ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta
    12061 gctatcgatc agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac
    12121 cgtgggacgc cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca
    12181 tcgatttccg actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa
    12241 ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga
    12301 acacccatgc aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta
    12361 aaagttggag gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct
    12421 attttgtcct attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa
    12481 ggagattatc atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt
    12541 gaaggacagc agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc
    12601 ccagaaggat tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat
    12661 caaagtctgg agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac
    12721 acaaaacgtc gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa
    12781 taatcttctt cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg
    12841 tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata
    12901 ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt
    12961 aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca
    13021 ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtatag
    13081 tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg
    13141 atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag
    13201 aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat
    13261 gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt
    13321 gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg
    13381 acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg
    13441 atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg
    13501 tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat
    13561 tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt
    13621 gcctgttctt caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata
    13681 atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg
    13741 actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg
    13801 ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc
    13861 gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc
    13921 aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca
    13981 ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca
    14041 atcattcata taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac
    14101 catatgataa attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat
    14161 cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg
    14221 tatattggcc atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc
    14281 cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac
    14341 aaaagcaatc gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc
    14401 ccacgattat gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt
    14461 ttataatgat gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg
    14521 caatgtccat acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg
    14581 acatgatcat gaccatgaac acggggaaga aggccataca catgagctag acccacatgt
    14641 atggctagct ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga
    14701 agcttaccca gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact
    14761 tcaagcactc catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt
    14821 cacgcaacat gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat
    14881 tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt
    14941 tgttaaagaa catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc
    15001 cagaacgtta gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt
    15061 gacgaatgaa caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga
    15121 agcgttgaaa aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa
    15181 agaaaagagc gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc
    15241 agattgggca ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc
    15301 tgtttttgaa tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata
    15361 ttatacgaag ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc
    15421 atttacgttt aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat
    15481 cttgacctac gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga
    15541 aagtggcgct tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga
    15601 acattttcat ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa
    15661 ttggccaaca ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt
    15721 gcactgatag gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa
    15781 actattcggt tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt
    15841 ctcgccgttt gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt
    15901 attgataaca taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt
    15961 gatcttctca tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc
    16021 tcctagcgaa gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt
    16081 aaactctatt ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt
    16141 gctcaggata tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc
    16201 agtcggttcg atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata
    16261 gattattttt cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat
    16321 ttcactgtct tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt
    16381 ggctgcattg agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt
    16441 ttcaatcatg attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc
    16501 ttcacaaata taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt
    16561 ttgagaaaaa cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt
    16621 caattcatcc aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa
    16681 ccctttttgt ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat
    16741 catcacggaa tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact
    16801 agctccttca atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg
    16861 tttcgtatcc agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg
    16921 tgccatcatg agttttaatg acagtaaact acggatctca ggaactttca tattatccaa
    16981 catacttaga ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc
    17041 ataataagac cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag
    17101 cagattttgg taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt
    17161 gatataatcc tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt
    17221 agagattttc caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa
    17281 actcgtattc aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat
    17341 gatatagtat gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt
    17401 gtgaaaccaa gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc
    17461 ggataagaca atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa
    17521 aacaaaaaaa tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa
    17581 aggaagcgcg taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag
    17641 cattcctata gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa
    17701 aatacaagaa atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat
    17761 caataagata caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa
    17821 gtacagctcc cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt
    17881 tttcagggat tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag
    17941 ataagttagt taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc
    18001 aagtagtttg taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag
    18061 gtagaaaagt aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg
    18121 atcgccttct ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct
    18181 cttgatccat agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg
    18241 aaggagatag tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt
    18301 gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg
    18361 cagatataag aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag
    18421 actagaacga ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact
    18481 tggacataca ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg
    18541 ttcacaactt tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa
    18601 taaaaaacgt cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct
    18661 aaaaaaatct tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag
    18721 tactttttat gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt
    18781 ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca
    18841 tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat
    18901 gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat
    18961 aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata
    19021 aaatgttgag agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata
    19081 atgttaaaaa acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag
    19141 gagaatactg tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt
    19201 catgaatagg agttactttt ggctactttt agagaagtag cttttaagga gcagataaag
    19261 tcaaggcttt tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa
    19321 ggatgccgat aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa
    19381 ctgaaatgct tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg
    19441 aagtattgcc gctattttat tctaatccag aaaagtatac ttttttatta cagattttct
    19501 ttttgaacaa acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag
    19561 accgctccat ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt
    19621 ctgaagtaga agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag
    19681 aaatttcgcc acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta
    19741 tgttagcgag aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac
    19801 tctattccta ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata
    19861 tatgtcccaa aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg
    19921 gagaacaaat cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg
    19981 cccagtcgat ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat
    20041 aatataaaaa ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct
    20101 tagttttttt gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata
    20161 aatgaaatgt ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat
    20221 gggcgccaaa cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa
    20281 cgacacttag atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat
    20341 catataagtt cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac
    20401 gcccgttgat tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg
    20461 ttactaatgc gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt
    20521 cataatatag aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta
    20581 gatactttgt tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa
    20641 cacttagcca ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag
    20701 cagaggcaaa agcaccatat tggatataag tataactgtc catggctcct ccgataaatg
    20761 tcagcaaaag tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct
    20821 tttatgaata tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt
    20881 tcagaaaact aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa
    20941 agaaaaaata gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga
    21001 aagatgggaa ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat
    21061 tgaaacgctg ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa
    21121 tcattgctac ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac
    21181 aagtaaaaga aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata
    21241 atcgcttcaa tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa
    21301 tcatagcagg agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga
    21361 tgacgcaaaa acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg
    21421 caaatgaact aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg
    21481 gaacattcca tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag
    21541 tagcaatgca aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg
    21601 ttttgcaaca gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa
    21661 cgattgcttc tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc
    21721 agttagttaa agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac
    21781 gactgtatgg attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt
    21841 tcaatgatgt actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata
    21901 tcggccaaaa atatgattat ctattcattg atgaatttca agatactaat ccacttcaat
    21961 gggaaatcgt gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg
    22021 atgatcagag tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa
    22081 aagaatatcc taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg
    22141 tccaaggagc aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga
    22201 ttccagcaca aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag
    22261 aaagtcagtg gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg
    22321 aatcaatcat attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga
    22381 aaacgaatat tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca
    22441 agtctttcca acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa
    22501 agaaaaatca ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa
    22561 aaagtgaatg cgatcaatac ttttctccta aatatcaaaa tatatccgtt gcagattata
    22621 ttttgtcgat aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag
    22681 cgttgcgtaa aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc
    22741 tgccacgtat agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa
    22801 agcagcatgc tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta
    22861 aagaattaaa aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc
    22921 agagcattca caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg
    22981 aaggaagttt gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa
    23041 aagcagaacc agtaacgacg atagaagaag aacgaagact attttatgtt gcgatgacta
    23101 gagcaagaaa tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag
    23161 tatcgagatt tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag
    23221 ggtgtgacag aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca
    23281 aatttttgtg aaatttcagc ttattttcga agaagctatt tctggaacac agtttattcg
    23341 gaaaagggag gtgtgacaag aattttgtca cacctccctt atttattttg aaaatgagaa
    23401 tactattatc aaaatagaga atatatagaa aaattctcat tattcaaacg taaataatca
    23461 gttattttga ttttttttat attttattag ctaataattg gtattttaaa agaaattact
    23521 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
    23581 gctgtacttc ctgtagaaaa gcaaaagcat ggttgcaagc gaatcaactt gaatttgaag
    23641 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga
    23701 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
    23761 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
    23821 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
    23881 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
    23941 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
    24001 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
    24061 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
    24121 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
    24181 attgcgattg acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat
    24241 accaatctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
    24301 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
    24361 ctgagcattt catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
    24421 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
    24481 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
    24541 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
    24601 ttgttgacat gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg
    24661 tgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa caaatgataa
    24721 gttatgataa atgagttgct gtaagatttc gaaaaacgaa atcaaaaaaa cttttaaaaa
    24781 agagttgaca attacttgtc tctctgatat gatataaaag ttgctacggc agccaaaaac
    24841 gaatcgggga acactcctga tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc
    24901 aaatgtgtag ggcgtcttga ttcaattcaa gacaacaaac a
//
LOCUS       DACDVT010000035        24756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDVT010000035 DACDVT010000000
VERSION     DACDVT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.035"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IWL52_001467"
     CDS             <1..572
                     /locus_tag="IWL52_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9778038.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IWL52_001468"
     CDS             576..1217
                     /locus_tag="IWL52_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778039.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDFIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IWL52_001469"
     CDS             1214..2131
                     /locus_tag="IWL52_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9778040.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IWL52_001470"
     CDS             2128..2799
                     /locus_tag="IWL52_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778041.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IWL52_001471"
     CDS             complement(3340..3828)
                     /locus_tag="IWL52_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9778042.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IWL52_001472"
     CDS             complement(4029..4295)
                     /locus_tag="IWL52_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9778043.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IWL52_001473"
     CDS             complement(4295..4558)
                     /locus_tag="IWL52_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9778044.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IWL52_001474"
     CDS             complement(4551..4823)
                     /locus_tag="IWL52_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778045.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IWL52_001475"
     CDS             5034..5219
                     /locus_tag="IWL52_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9778046.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IWL52_001476"
     CDS             complement(5361..7184)
                     /locus_tag="IWL52_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9778047.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IWL52_001477"
     CDS             complement(7782..10487)
                     /locus_tag="IWL52_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9778048.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IWL52_001478"
     CDS             complement(10793..11740)
                     /locus_tag="IWL52_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9778049.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IWL52_001479"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IWL52_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9778050.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IWL52_001480"
     CDS             complement(12375..12737)
                     /locus_tag="IWL52_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9778051.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IWL52_001481"
     CDS             complement(12748..13869)
                     /locus_tag="IWL52_001481"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9778052.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IWL52_001482"
     CDS             complement(13885..14235)
                     /locus_tag="IWL52_001482"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9778053.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IWL52_001483"
     CDS             complement(14365..15876)
                     /locus_tag="IWL52_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9778054.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IWL52_001484"
     CDS             complement(16198..17550)
                     /locus_tag="IWL52_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9778055.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IWL52_001485"
     CDS             complement(17703..18779)
                     /locus_tag="IWL52_001485"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9778056.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IWL52_001486"
     CDS             18975..20720
                     /locus_tag="IWL52_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9778057.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IWL52_001487"
     CDS             complement(21000..21437)
                     /locus_tag="IWL52_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9778058.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IWL52_001488"
     CDS             complement(21421..22794)
                     /locus_tag="IWL52_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778059.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IWL52_001489"
     CDS             complement(23116..23604)
                     /locus_tag="IWL52_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9778060.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IWL52_001490"
     CDS             complement(23626..24228)
                     /locus_tag="IWL52_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9778061.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            24327..>24756
                     /locus_tag="IWL52_001491"
     CDS             24327..>24756
                     /locus_tag="IWL52_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9778062.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         7145 a         5230 c         4582 g         7799 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgattttatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttccaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc ttatgatgtg caccccaaaa gttagactag
    24301 aaatctaatt aaaaggggtg ctttttttgc ggaaatatac atttgttttt aagaaaaaag
    24361 tagtttcaga ctatttaaat aacgaaggcg gctacaaata tcttgcacat aaatatcaaa
    24421 taaatcgtac actggttaga cattgggtaa ggatttataa ctatcatggt tgggaaggct
    24481 tggttggagg tggcaaaagc tacactacaa aatttaaact tgatgttata gaatatatgg
    24541 aaacaaatgg tctttctatc caagaaactg ctaaaaaatt taatatcggt tcaaatagaa
    24601 ctctaagtaa atggatagag caatatgaag aaggcggtgc ttcttcactt gagagccaaa
    24661 aaaggggcag aaaaattagt atgaattcca agctaaacat tcctaaaaaa cttaaagatg
    24721 agtctcttga agaagaagtt attcgtttaa gagcag
//
LOCUS       DACDVT010000036        24577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDVT010000036 DACDVT010000000
VERSION     DACDVT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.036"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(153..551)
                     /locus_tag="IWL52_001492"
     CDS             complement(153..551)
                     /locus_tag="IWL52_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9778063.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IWL52_001493"
     CDS             complement(548..1321)
                     /locus_tag="IWL52_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9778064.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IWL52_001494"
     CDS             complement(1318..2001)
                     /locus_tag="IWL52_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9778065.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IWL52_001495"
     CDS             complement(2168..3055)
                     /locus_tag="IWL52_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9778066.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IWL52_001496"
     CDS             complement(3052..3921)
                     /locus_tag="IWL52_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9778067.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IWL52_001497"
     CDS             complement(4058..5344)
                     /locus_tag="IWL52_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9778068.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IWL52_001498"
     CDS             5617..6195
                     /locus_tag="IWL52_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9778069.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IWL52_001499"
     CDS             6195..7124
                     /locus_tag="IWL52_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778070.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IWL52_001500"
     CDS             7121..8857
                     /locus_tag="IWL52_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9778071.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IWL52_001501"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IWL52_001501"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9778072.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IWL52_001502"
     CDS             complement(9753..10262)
                     /locus_tag="IWL52_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9778073.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IWL52_001503"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IWL52_001503"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9778074.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IWL52_001504"
     CDS             complement(11563..12654)
                     /locus_tag="IWL52_001504"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9778075.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IWL52_001505"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IWL52_001505"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9778076.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IWL52_001506"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IWL52_001506"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9778077.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IWL52_001507"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IWL52_001507"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9778078.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IWL52_001508"
     CDS             complement(16617..17591)
                     /locus_tag="IWL52_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9778079.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IWL52_001509"
     CDS             complement(17572..19455)
                     /locus_tag="IWL52_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9778080.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IWL52_001510"
     CDS             complement(19473..21698)
                     /locus_tag="IWL52_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9778081.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IWL52_001511"
     CDS             21813..22847
                     /locus_tag="IWL52_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9778082.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="IWL52_001512"
     CDS             complement(22914..23927)
                     /locus_tag="IWL52_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9778083.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="IWL52_001513"
     CDS             complement(24005..24193)
                     /locus_tag="IWL52_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9778084.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7063 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaa
//
LOCUS       DACDVT010000037        24413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDVT010000037 DACDVT010000000
VERSION     DACDVT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.037"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWL52_001514"
     tRNA            8..81
                     /locus_tag="IWL52_001514"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWL52_001515"
     tRNA            101..173
                     /locus_tag="IWL52_001515"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWL52_001516"
     tRNA            201..274
                     /locus_tag="IWL52_001516"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWL52_001517"
     tRNA            288..361
                     /locus_tag="IWL52_001517"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWL52_001518"
     tRNA            385..474
                     /locus_tag="IWL52_001518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWL52_001519"
     tRNA            487..560
                     /locus_tag="IWL52_001519"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWL52_001520"
     tRNA            579..654
                     /locus_tag="IWL52_001520"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWL52_001521"
     tRNA            656..726
                     /locus_tag="IWL52_001521"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWL52_001522"
     tRNA            740..813
                     /locus_tag="IWL52_001522"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWL52_001523"
     tRNA            838..925
                     /locus_tag="IWL52_001523"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWL52_001524"
     tRNA            947..1018
                     /locus_tag="IWL52_001524"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWL52_001525"
     tRNA            1041..1130
                     /locus_tag="IWL52_001525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWL52_001526"
     tRNA            1143..1216
                     /locus_tag="IWL52_001526"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWL52_001527"
     tRNA            1235..1307
                     /locus_tag="IWL52_001527"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWL52_001528"
     tRNA            1311..1383
                     /locus_tag="IWL52_001528"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWL52_001529"
     tRNA            1388..1458
                     /locus_tag="IWL52_001529"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWL52_001530"
     tRNA            1471..1544
                     /locus_tag="IWL52_001530"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWL52_001531"
     tRNA            1556..1627
                     /locus_tag="IWL52_001531"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWL52_001532"
     CDS             1893..3086
                     /locus_tag="IWL52_001532"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9778085.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWL52_001533"
     CDS             3216..4688
                     /locus_tag="IWL52_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9778086.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWL52_001534"
     CDS             4771..5559
                     /locus_tag="IWL52_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9778087.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWL52_001535"
     CDS             complement(5626..6294)
                     /locus_tag="IWL52_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9778088.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWL52_001536"
     CDS             6409..8121
                     /locus_tag="IWL52_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778089.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IWL52_001537"
     CDS             8124..9893
                     /locus_tag="IWL52_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778090.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IWL52_001538"
     CDS             complement(10098..10664)
                     /locus_tag="IWL52_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9778091.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IWL52_001539"
     CDS             complement(10664..11620)
                     /locus_tag="IWL52_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9778092.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IWL52_001540"
     CDS             complement(11620..12276)
                     /locus_tag="IWL52_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9778093.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IWL52_001541"
     CDS             12646..14664
                     /locus_tag="IWL52_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9778094.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IWL52_001542"
     CDS             15091..17505
                     /locus_tag="IWL52_001542"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9778095.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IWL52_001543"
     CDS             17853..19406
                     /locus_tag="IWL52_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9778096.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IWL52_001544"
     CDS             complement(19787..21571)
                     /locus_tag="IWL52_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778097.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IWL52_001545"
     CDS             complement(21586..23307)
                     /locus_tag="IWL52_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778098.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IWL52_001546"
     CDS             23407..24168
                     /locus_tag="IWL52_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9778099.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7584 a         4468 c         5161 g         7200 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaattccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagtgaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcagcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggt
//
LOCUS       DACDVT010000038        24199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDVT010000038 DACDVT010000000
VERSION     DACDVT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.038"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(126..578)
                     /locus_tag="IWL52_001547"
     CDS             complement(126..578)
                     /locus_tag="IWL52_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778100.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(585..1133)
                     /locus_tag="IWL52_001548"
     CDS             complement(585..1133)
                     /locus_tag="IWL52_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9778101.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1178..1510)
                     /locus_tag="IWL52_001549"
     CDS             complement(1178..1510)
                     /locus_tag="IWL52_001549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778102.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1524..1754)
                     /locus_tag="IWL52_001550"
     CDS             complement(1524..1754)
                     /locus_tag="IWL52_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778103.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1976..2518)
                     /locus_tag="IWL52_001551"
     CDS             complement(1976..2518)
                     /locus_tag="IWL52_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778104.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2539..3630)
                     /locus_tag="IWL52_001552"
     CDS             complement(2539..3630)
                     /locus_tag="IWL52_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9778105.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3638..5848)
                     /locus_tag="IWL52_001553"
     CDS             complement(3638..5848)
                     /locus_tag="IWL52_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778106.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5864..8392)
                     /locus_tag="IWL52_001554"
     CDS             complement(5864..8392)
                     /locus_tag="IWL52_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9778107.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8444..8854)
                     /locus_tag="IWL52_001555"
     CDS             complement(8444..8854)
                     /locus_tag="IWL52_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778108.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8867..9085)
                     /locus_tag="IWL52_001556"
     CDS             complement(8867..9085)
                     /locus_tag="IWL52_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778109.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(9082..10074)
                     /locus_tag="IWL52_001557"
     CDS             complement(9082..10074)
                     /locus_tag="IWL52_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9778110.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(10153..11064)
                     /locus_tag="IWL52_001558"
     CDS             complement(10153..11064)
                     /locus_tag="IWL52_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9778111.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(11083..11427)
                     /locus_tag="IWL52_001559"
     CDS             complement(11083..11427)
                     /locus_tag="IWL52_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778112.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11447..12003)
                     /locus_tag="IWL52_001560"
                     /pseudo
     CDS             complement(11447..12003)
                     /locus_tag="IWL52_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12084..12584)
                     /locus_tag="IWL52_001561"
     CDS             complement(12084..12584)
                     /locus_tag="IWL52_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778113.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12585..13130)
                     /locus_tag="IWL52_001562"
     CDS             complement(12585..13130)
                     /locus_tag="IWL52_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778114.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(13127..14383)
                     /locus_tag="IWL52_001563"
     CDS             complement(13127..14383)
                     /locus_tag="IWL52_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9778115.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14744..15055)
                     /locus_tag="IWL52_001564"
     CDS             complement(14744..15055)
                     /locus_tag="IWL52_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778116.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(15078..16388)
                     /locus_tag="IWL52_001565"
     CDS             complement(15078..16388)
                     /locus_tag="IWL52_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9778117.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(17002..17454)
                     /locus_tag="IWL52_001566"
     CDS             complement(17002..17454)
                     /locus_tag="IWL52_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778118.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17456..17821)
                     /locus_tag="IWL52_001567"
     CDS             complement(17456..17821)
                     /locus_tag="IWL52_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778119.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17920..22203)
                     /locus_tag="IWL52_001568"
     CDS             complement(17920..22203)
                     /locus_tag="IWL52_001568"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP9778120.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22350..22589)
                     /locus_tag="IWL52_001569"
     CDS             complement(22350..22589)
                     /locus_tag="IWL52_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778121.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22679..22951)
                     /locus_tag="IWL52_001570"
     CDS             complement(22679..22951)
                     /locus_tag="IWL52_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778122.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(23034..23285)
                     /locus_tag="IWL52_001571"
     CDS             complement(23034..23285)
                     /locus_tag="IWL52_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778123.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         7164 a         5228 c         3517 g         8290 t
ORIGIN      
        1 tgttcttcta aacaatcatc tatactcata taatcactcc actaattaat ctccccaaaa
       61 agacacagca ttaagagata taccaacaca acagtgcaaa taactctaca tcttccttcg
      121 caaccttatt tcaataaacc ataaaaatta ttttttaata gaaactgaaa attaaaacta
      181 gaaaaagtag ttttaccgaa tgtaagagca tcatcaccat caatatagaa atacattctt
      241 ttccagttac atttaatttt taaacaaaat tctccccaaa aaggaacttt ttctagtgaa
      301 aacttggtaa tctctttgta attaattctt gttaaagaat aattaaacac ttcattttta
      361 cttgttattt tttcattgac tataactagt tcatctggta aaaaaattaa ccaacaaaca
      421 tcgacaaaca gtaaatgata taatccattt ccacctccaa gcatattcaa tactggtgaa
      481 catgccttag aaacacgaat atagttatgg tttaaataga tattttcttt aaacattgct
      541 tgtttaaaag ccatatgctc ttgttctaaa cttttcataa acttttactc taatgcacag
      601 ctagcagcag ctgctgcaat tcctgctaca atatttacct tagctaactc tttagctgca
      661 gcttttccta attgtttttc tgcaattttt aataataaat ccgcagcttt ttcaaaatta
      721 aaagctttta atgcttcaat aaaatctccc tgcgtaccta tagcccaagc cactcctcct
      781 ccaggaagag aattaatggc tacacatcca gcatatcgtt tccaatcaac tttctccatt
      841 ctaggaacaa taatatgttc ataaaagact cttgctgctc ttccttgttc gttatcttga
      901 ctgattcgtt cacgaatagc accaaaatcc tttatatggt atacaccatg ttcgtctaaa
      961 aaaccaactg tatcaaaata aaaagttaat tcatctacta atttattctt tatttctaaa
     1021 tcaactgtat tcactttttc tgtggcatac acacttgttc cagtactaac aaaagtactt
     1081 aaacctaata agaaaacacc taaaacacca aatattttaa ttattctttt cataatttct
     1141 cctccataaa aaataagtta ctttttaaat atcttgttta tgaattcatt ccccaaaggc
     1201 cttttaagat tgatacagca gtccgttcac ttcttttaga cttgtcatac aagtctttta
     1261 aatgatccca tatcagacta gtttgtctat ctagcttcaa tccattaaac gctaatgaac
     1321 ggttcatttt ttctattact ttagataaag ctacagaaat ttttatacct tcacaacgcg
     1381 catcttggat ttcaataata gtatcatcaa ttagtttttg aatttcttgc ctatcaacat
     1441 attcaatctt agaaagagaa tcttttaaag ctactaatga ttcaacgatt tcttttttct
     1501 cttcagacat tttcttcctc cttctagtga cccgtaaagc catgtagagc agagtccatt
     1561 gaaccagtaa ttacactatc tacaacgcca ttccaattta ctctacattt tccatttttc
     1621 agatttgtcg gtccatacgg atagcctttt tgacattcaa tgttcggatt ataccagata
     1681 ggattatacc attcagccgc agaagcagat tcagtcgata caaacaatcc cacaccaaca
     1741 aatccaatac acatcacact atagccaatt agttttttca tctcgtatcc atctcctaaa
     1801 aaatagaata gttcttatag ttattcataa gacatatttt ttccgttttt gttttatcct
     1861 ttctgtccta taacaacttt tttcaagtaa agaatgacat agtttttctt aaaaaaacag
     1921 tctgtttttt tctatttctt cggtgtataa taaatatgag tgttcatttt ttattttaat
     1981 acgcactccg tacataataa atcttgatcg catcaatctt ttgagcttct tgatctactt
     2041 tcaattgaag taatacaaat tgagtaacct tattcattgt ttcttctcca aataacaaat
     2101 attgatccgg attctctata tcttgtgtca cctggtaaag tttcccagtt gttttcattt
     2161 gtacatgcgg gtctacatca atactattgt ctttaataca acgatctgtc atatagtccc
     2221 tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt
     2281 ttaagaaatt ttttgtcatt tcatctattt gcatttgttg cgctgtctcg tcttctaatg
     2341 aaccaatttg actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt
     2401 cgattgtttc ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac
     2461 aaccagtaag acctatcaaa cataaccaac taaatactaa aattatccct tttttcacgt
     2521 ttctagtcac tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt
     2581 gtacgtgcgt tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga
     2641 gaaatgttgt ccgccatatg aaacatctgt tataattccg gtgtgaccat agattccagg
     2701 agacaatgca ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt
     2761 tgggttcatg attacttgcc aaccataggt tgtccagtta taatcttccc ctattaacat
     2821 cgcaaacatg tgtccagaac ccattaactg tgggccacct aattgagata cataccatgc
     2881 cgtaccacca taacattccc cattatttac ggatcgtcca atgatccgat ccaaaacctt
     2941 cgcattgcct ttactttttc ctgcaataag cgaagttttt tttgttggta ggctattcca
     3001 atgcttgatc gcaaaagtat catattccgt taagttatag gacgttatga tactcattgc
     3061 tcctcgataa tacggactcc cttcggtaat acctgctgcc gcatagcctc cacgacctag
     3121 aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt tgctttggct
     3181 cgccaccata aagccataat agtcataaca agcttttaag ttcggaaact tcatatagaa
     3241 accaccttca gaaccaccaa ttccacgtgc agaaccttgt gggtagggac aaccaggaaa
     3301 ccaggtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc cataagctga
     3361 ttcatagcct aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc
     3421 aactgctgct tgcgcatgtt catctgtgat tggaccacgt cctttaatct ctgaaaaaat
     3481 cggtaatcct tcggtatatt ccccattaaa agcgacagct agacctgtag cttctcctcc
     3541 atgctcagaa agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa
     3601 aagaagacaa cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt
     3661 tttggactgt tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg
     3721 gcagtgtttt aggatccttt tctatttttt ccgattgctc tgttggctta tctaaagaag
     3781 aaaatgcagg ttgtaacgat tttggtgcgg aaatcgacgg tttactttca gacgaacgtt
     3841 gccccggttg ttgcacagaa cgatttcctg atagagtggc taccgacgaa ttaggaacag
     3901 agtattggtt caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca
     3961 cttggcgaat ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc
     4021 cagaaggcaa cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc
     4081 tttgattatt cccttgttgc ttactcaatt gtgccaatga ttgtacctgt tgttttggtc
     4141 ttactgaatt catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg
     4201 ttttcgattt ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac
     4261 tataaggcaa ggcttgattt gaacgcccac ctagttcaaa gggattaacc acttgcctcc
     4321 cttttaccga ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg
     4381 cagaagcgag tggattcgtt gctcgataca cattccctgc taatcctgca cctttgattg
     4441 ccaaattttg tccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat
     4501 gacttgctat gtttttcgtg ttgtttatag tttgatctgc agctcctaac cctagtttct
     4561 ttaataaatc tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat
     4621 ttcctaaaaa accaaaaaca gtattcattg gaattaaatc gtctactatg ttatagatca
     4681 aaaatgccac caaaataatc acactgtaaa aagatttttg aaacatcaag cccaaaaagg
     4741 actttaaggt attcatgagc atataatcaa atactggaac aaaagaagcg agtaatgaaa
     4801 aaggaatcac aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa
     4861 tcactacaag tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg
     4921 ctttataaac agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat
     4981 cttctcctgc agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt
     5041 ccttggctag aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg
     5101 tcccataatt taacaatata tacggatcaa caacagccgc tttataataa agttggcgca
     5161 ctttttgaat ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg
     5221 ccaattcatc tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat
     5281 catttaaccc tttggtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc
     5341 aaaccattga aaaaatgaca acgaagagaa atcgaataaa caccattttt gcgtaatgtt
     5401 tccctctaaa tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac
     5461 caataggatc gtatattttt gaataaatag tatccgaaaa agtgaaaaac tggtgaatta
     5521 actcgttcat tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa
     5581 tcaatttact taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg
     5641 ccccaaataa cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat
     5701 caatatgaaa tttcccaatc tcatagatcg attgatccac ctgcttttct gccgcataca
     5761 ccaatccacc aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt
     5821 tcttttcctt atgctttata taagccattg tttcacctca tttctattgc atgattaacg
     5881 tatcctgttc ttctacttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca
     5941 tttcttcaaa caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc
     6001 aacactgtcc tttaatcatg tggctcattt cagctaagtt cgcttcattt acttccaaac
     6061 gtaaatgatt caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa
     6121 aattcccttt ttctttatct gaacctaagt caccaataaa ttgtgtatct aacactaatt
     6181 gattcatctg ggaacgcccc acacgtgcca ttgctgcaat aatactacgt cctgcgttcg
     6241 aattattaaa tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt
     6301 ttccaaacat ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg
     6361 catctgtata tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta
     6421 aaatcgttgt tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac
     6481 gtaaaatcga atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt
     6541 gttccatttg ttctaaatag tcgaccacat gcatcaagcc aacttgttgt ccggcttctt
     6601 tttgaactaa aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac
     6661 ctaacggact taactcatca atcatatcct gggctacttg tttcgcatct actcgggata
     6721 aacaaacaat tggatctaat acaccataat tacggggatt tgtagcatct aacgtgacat
     6781 aactcatttt ttgcaaatgt tctacaaaca aagggtaatg tttctgataa tacgggtctt
     6841 gaatgagttt cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta
     6901 cattcataaa cgaactatac aaaaagagca agccaactaa aaaggtcttc cctttaccgg
     6961 tttttccagt tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa
     7021 tggctagaag attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg
     7081 ttttttctcc tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg
     7141 attccatacc taattctgat actgctgaaa acaaccattc gccaactgct tctacagttg
     7201 ttcggtgctt ccaatgtaac tgccgtccca atggttgccc taataaacat ttatagaaca
     7261 attctcgttg atcatttttt gcacggtgga tatggataat cgtatgaagt tgttgaataa
     7321 ccgttgcact attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga
     7381 tccaatcgac gacaggttgc ccattatcta ctcgttgctt taaatgtttt actaatcctc
     7441 gaacagttaa taagcgttca ctaggttctt catcattttg aaccaattcc ttatgctctg
     7501 ttttgaaccg ttccgatagc caattcagtt ttaaaccaat acctccctgt cctttcaagc
     7561 gtggatagtg aattttcatt cgcaattctg ctgggaaaga aagatcctga gcaaattgaa
     7621 atagatcaca atttctcata ttttcaggtg tttccccaat aggcaacaat gctaaataac
     7681 tactccctaa ctcatcttct aattgaagaa ggcctttatg ttccttcggt atacgtacat
     7741 acgttccttg attgtctttc agtacttccc aattattttg ttcttctact ttgtgagcca
     7801 ttcctcgaat atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa
     7861 tagaaaagct ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca
     7921 aagtttccaa atcatctact tctaatccaa ataagcgtgc taaacttcct gctaaatcta
     7981 accagattag atttattaca tcatgaaaac tctccaccgt tgctttttct ttgactttga
     8041 ttcctaaaat aaatgaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt
     8101 cttttgcata atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt
     8161 caaacttcgt ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat
     8221 ctgctaaaag caacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg
     8281 tgttttttga aattgttcgt ggaaaaacat gatagtaggc ccagatacaa tcctctccat
     8341 ttaacaaaca atttttcttg aatgctttta ctggttcttt tacaagcacc aatgaatttc
     8401 acctcccaat aaaaaaggaa caaccagttg attgtccctt tttctaatcc tctaaataaa
     8461 catacttatt cacaaaaagt aattcttcca atgcattgtt tgataccttg tcccagttat
     8521 aatacacaca atcaccattc atcatacgaa tcatataaca aaaccaatcc cataaataaa
     8581 aatgcacttt ttttccatca ggttttagtt ttgccagtgc taacacacat aaatagggga
     8641 gtcctatata aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg
     8701 aacgtaaaaa taaatattca aagattacga ccaacaaaaa agttgctgca ttgactaatg
     8761 caactggaaa tggaagacgc caattgcttg taatctgttg tacgcgataa ggttccgcat
     8821 aaacaatcga ataatcataa ggttcccaat ccacttcact atcctcctaa tccataaata
     8881 atttttcaat gattttacca aacgcatcaa atacacgtgt tggatcattt aagaagaaat
     8941 aaactgcggc acctgctgcg atagatatta atgcatttcc tacttttgat ttcataaaat
     9001 ttcgcaatcc cagataaaca accaccatta ttaaaaaata gccaccttgc gttaatataa
     9061 aatctacaat tcccttcagt gtcatttccg ttcttctcct ttcctatcca accaattatg
     9121 tgttaatttt tcaacataat actgatttgt ttttttcgta atgacaatcg tcatttgttc
     9181 tttttgtaaa agtgacgtat caccatctcg aaagacgacc gttgctttta caataaaagg
     9241 ttttttcgtt tctttataac gcaatacttt catctctact tgatcaactt tagctgtttg
     9301 attgagtcct tcaggttctt tcatcatgta tgacatatct tttgcattgt ttttcgcata
     9361 cttatctaag aaatcttcca caaatccttc aacatctttc gcttccgacg catcaaccgt
     9421 ttcgtataat gtgtcatcat aagtaatcat tcgttgtttt tctgtattct ttggtagtgc
     9481 cgtaaaataa ggataagcaa taatacgaca gcccttgttt gaaatagaaa agggaagatt
     9541 gagtacttgt gtgattgtca cacttgtttc tttcttactc tctggattca tcgagcgaat
     9601 atcataagtt actttataac tagcagttgg aatggtttca atttctttta atccaataaa
     9661 cgaagcagca gttaattttc gaacacctga aacacttcct ggttcttgaa taaagttttg
     9721 tgcataaaac tcttttttta atgtttcttg acgttgttgt tcttgttcat gattatcgaa
     9781 tgtattcata tacaaagcaa caaaacgatt cataaaggat tcaatttcag gtgtgtacgt
     9841 taattctgac cgactttgta cttctttctt ttgtaattgt tggatttcat ctttcaaaaa
     9901 cgacacttga accttcgttt tcatggattg aaaaaatgcc aagccccctg aaaaacaaag
     9961 aaataaaacc aaacaccaaa tccatttcac tgcttttttt gaatggattt tcttttgctt
    10021 cggtactttc tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt tcataagcta
    10081 ctccttctag tatgttttta ctagtacgta cgattgacaa cgataagctt gtttttctga
    10141 aaaatagtcc attcaattac cctcattttc tattcctttt ctctttcttt tatatccaac
    10201 aaatccagca atacttgtca acactcctcc tagaacagaa agtgcaacat gtatttcttc
    10261 accagtcttt ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa tattgccact
    10321 tggaagtttc tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac tatcttggat
    10381 taatggaata ccatctttta caccaacaat tgtttgatca cctgctactt ttaatggggt
    10441 ggttggtgtt ggaatatggg tcaattcctc tacattacta gtgacaatcg gtacactcat
    10501 tccttgagaa gtattcgtcg taatttcacc gcttggggtt accgatactg tgacttcttt
    10561 tgctggaaca ataggtttat tttcttttgg aattgcttct gatttttcaa caggttgttt
    10621 cggttcttct ttttgttctt cactggtaga cgacatcgtt ggctgttccg tacttgacga
    10681 atcagtattc ttttcagaag aactggttgt tcctgattgt tccggagttg tgactatact
    10741 tgaatccgtt gttgttgaac tatgcacacc ttcttcttcg atcgaatcag tcggtaaagt
    10801 tgattcagga aactctggtt ctggtgtcac tggctctttt tcaggtttct ctgactgcaa
    10861 ttgttcaggt tctgctggtt tttccgttgt tcctccattt agctctgtct caacatctgg
    10921 taatccttcc cctggcaatg tttctacaat ttctgactta ggtgtttcta cagtttcatc
    10981 tgcataaacc aatgaactcc ctaattgaaa cgaaaataat gaaattccta gtgttgttac
    11041 gactgctaat gttgtttttt tcatgattat tcctccaatt ttttatgcaa ttcgtgtttt
    11101 gttcatagac gccgcactaa tattatctga acgataaaag aactcccgtc cgttttcttc
    11161 taacccaaca tatggattat ttttgaataa ttgaatcaat agttcactgg tgttgtattt
    11221 tggatagttt gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt caacaagtaa
    11281 ttcataacga taccgcttca atccttttcg ttgttttaaa ggatctctat accaacttgt
    11341 ccatcgttcc attcgtggat agagatagac acgatacaca tagaacaatg ccataccaca
    11401 taccaccaaa aataataatc gccacataaa aattccccct tttcttctac ctttcttgaa
    11461 tcatcgaaca ttcataccct ctatagagat ttgaaaaatc catcatcaat tgttcatata
    11521 aacgttgttg caaatagcga ttgtttcgaa tacataattc ttcatagcca tcaatcactt
    11581 gatcttcttt ttcatagcat cttgtagctc tatgacgctc agcttgcatg acataaccaa
    11641 actctgcacc atatcgttct actgttaata actgttgact acgcaataat gcaatataac
    11701 aatcacaaaa atgttcaacc gacatttgat ttttctcacg tttgtgaaat aggtattctt
    11761 tagaaagcac attattcgtt aaacaatctg ataaatagcg ataatctggc ggtcggttat
    11821 tttcatctgt gaatttttcc cataataaac tgatttttat tcgttcagcg gctagatagt
    11881 cttgttcgcg tggataaaat tcatcaatcg cacaaagttt tttatcacct gaataaacaa
    11941 atccatataa tgcgaaacga actcttcctt ttcaattggc tctcctaaaa attccagtga
    12001 cataccatct tctccttcct aaaaaaaata aaaaagcaga aaccgatttg gcttctactt
    12061 ttctttcctt ttatagctct tttttacgga agaatccgca aatgacattt tttcaaaaaa
    12121 tcaatcggca caagttcacg aaaacttgga gcaattttgg atagaatcac ttccccatct
    12181 tgtaaaatac aatcataaaa atcatcagtt acttgatact tacgcttctt acaataattg
    12241 ataaaatagt agcgatcatt ttctatttcc attcgttgtt ctactaagcg catacgaaca
    12301 tcatcataga aataagccac aaatcgccct tcaagttcat gtttttcaat gggatactct
    12361 tctggtaaca atctattttt tcgtcgttta tacattgctg gataccttag aagaagttca
    12421 tctaaacgtc tgtcagtata tggtaactga cgaatcaacg attctaatcg ttctttatac
    12481 agatagaata attggcgcaa gcaatagtgt accgcctctg gattttctaa tactgttgga
    12541 tcacgtgacc aatacgcacc aacataaatc ataatttttt tcatttagac aatcacacca
    12601 gcaatactaa tgaccgatcc acaataggta gaacaagtgg catagcgacc aatttcctct
    12661 aattctatct caatctcttc taaagaaaga tatgtctgtt cttctgttgg ctggcgactc
    12721 atttcccaag tgacagtcac caatgaaatt tcttccataa gaggtttttc gctgctaagt
    12781 gctgccacac agttttcata tatcttcatt ttatcgcagc cttttttaac cgctaaaatc
    12841 gcaggtattg gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc tttccttcgg
    12901 ctaaattcca actcttcgag ttcttgttcg tatcccttac gaacttctaa taagcgtgca
    12961 atttcttgcc agttctcttt ttcctgataa cgtccagctt ttaccgcttg ttcaatataa
    13021 ggaactcgaa aacgaaaata agacaaatca accatctttt catttaatgc ttcgattgca
    13081 gcctttaaat ccaacaggcg cagccattct gttgtcgtat atgcattcat gcaattttcc
    13141 ctcctgacgt tcccatccat tctagatagt tttgttgctc gtcatttagt tcggcattat
    13201 caatcccttc ttgtacaaag tctcttccta aaatacgatc tgcttcttgt aacaacttta
    13261 atgtcggagc tacttgtttc aaaaaccagc gttctgaacg atctaatgag aatttttctg
    13321 gctttactgt aaaatccaat tcttccatcg aacgaaataa ttttgcccat cgtttatcaa
    13381 caatccgtgc atcactttcc agtctatccc aaacaataag ttttgcatta attaaaccaa
    13441 cgccaatctc tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct actgcttgtg
    13501 ttgctttctc attggctaac cgtaactcat atcgattata aataccatat ttcgttaatg
    13561 cttctaattc tgaaattttt tctcggttcg caatttcaaa acgtttctca taaaaattaa
    13621 aaaagagagg actctttcgc gatccaaaat acaaagataa tccttcactt cgaaaatagc
    13681 catcatctaa aaaacaaccg ccaatattct ggaatctctt aaagtccatt tctaataaac
    13741 ctgctttttg tttttctagt aattcaaaga gatcgaaatg ttcttccgtt tccaatcccc
    13801 aaagttcatc taacgcaata tcgaatcggc tacattgcac acgtgtataa ccagcttcca
    13861 actttccttg atttttccaa atggaatata gccatgttcg ccaatctgtt tgttgttcac
    13921 gcatgacttc ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt acattttctg
    13981 ttacagttcg ttttgaatgc cagaacaccc agatattccc acgaacagaa agacagtcaa
    14041 aaagatacaa gcccgttgga cgtgtttcaa attctgtcag atccattcgt aatacacgtt
    14101 gaatcactgt tttgtagtct gtcccaaaaa aggaaagacg caaataatca atcattgctc
    14161 gaagatctat tccgcttttc atatacaatc gaaagcgttc cttgattcgt tgttcaaaat
    14221 cctctgggat ttttcttttc ccttgttcca tctttctaat gtaatctttt gagcagccca
    14281 tcaattgcgc aaatcttgct tgtgatagcg gctttttatc tccacttata ttatgaattg
    14341 cacttaactt ctttctgtag ctttttagct caatcggtgt cattttctcg ctccttttat
    14401 aaaataacaa aatcgatttt ttttaaccct ttagaaacac cgatatatca actagtcccc
    14461 ctcgtttttt gggaaaagta gtacaactgc tgaccttaga taatcacttg atacaaaagg
    14521 gtttataaaa cttttttcta tgtgttatgt tatttctgcc ccccatatta tatatcgggg
    14581 ggctttacac aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc
    14641 tagcgagtgc gcattatagg cccatgcttc gcagccttaa tgcgcaccgc caggcagagt
    14701 gcggctacca gactacgtct agagccctcc cgatagataa taactacaaa attaaatgat
    14761 ctaattttgt tatttcacta tatttttcag gagaaaaaac gcgataaatt gacaaagtta
    14821 aatcctcttc taaatctact cgataatgat acaatcgagt caatcgttct atatcttttt
    14881 tcgttagcaa tacttgatta ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc
    14941 ggtaatcagt tacttgttca atacgattca accaatcata aaacgttctg gaagaaagag
    15001 aaggattaat cattcgtatt gctgtagcta ctctataata acaactctct gccatttaac
    15061 ttcacctcct tagttgatta caaaatatgc gcatcagaaa aacttgttcc ttttggatct
    15121 tttgctaaat acatttggcg ctctagttgt tcttgtaaca actgattatc atctcccatc
    15181 gtttgatctt gactatcctc ttttactgta aaatcataga caatcatttc agtaaatttc
    15241 tcaaattctt tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt
    15301 acatcaaatc ctggaaaagc atatccacga ccaggttgat ctttgttgta aaatggctta
    15361 tttttaaaat cttctccaaa agtcattaaa taaccaatgg gagacaatcg accaacagaa
    15421 atacggaatg ttaaattatc ccgaacacca cttggaaaat attctgcatc tggtcgttgc
    15481 attgctaaaa taatgtacat ccctgcttga cgacccttta atactaatgg ctgaatcaat
    15541 ttatcgactc tatttctcgc ttccatatcc aacgaaccat aaaaagctgc ccattcatca
    15601 aaaaagataa aatgcggtgg taacccataa taagcataat ttttccctgg ttcatattta
    15661 ggatctttca tggttttcat ataggcatac cgttgattca tttcttttaa tgcatttcgt
    15721 aaacaaataa tcatttcttc atttattttg taaaacacat gtcctttgaa tacatcgacg
    15781 tcacctaatg ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt cccaacactt
    15841 aaacaagctt gaattaaaga atacaataaa taggttttcc caccaccgat tgcaccagca
    15901 atcaacatat ttggtgattg atcatatacc caatccaaag ttttcattaa atgaatacgt
    15961 ccattttctg ctatacattg atcaatagga atccgaccag aatctgaaag gaatacataa
    16021 ttgattcggg ctttttctcg ttcttcacga attaaatcac gcaaaaatac ttcagataac
    16081 aaatgaccca tttttaaaaa tcgttcgtga aaacgcccac catctaaagg aaaagaaacc
    16141 actaattctt gtttcttttg ataaaaataa acttttggta atctcatttt ttcccttgtt
    16201 tgtttctcac taacttgttt ctttgtataa acaaaccgat tgtcaattaa cattcgaaca
    16261 agtaactgac gccaataaac agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc
    16321 aaccctaaaa ttaacccact aaccagcaac aaatacaaca caatacttat tccaggaata
    16381 agatacattt gccaattcga aaacgtactt aaacgtggat aaaatacttt ccaactaaac
    16441 caacctaaca gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa ccataaataa
    16501 cgattgaaat agcgaattcg ccgaccacga tacttgaaaa aatgagcttt caattgtcac
    16561 attcctcctt tctgttttta cttttcctgt gctaaaaact tttgacaaaa agcaactaat
    16621 acaggaatat cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg atttttataa
    16681 gggtgtgcac acatattaca tattcgtact atacgaatcc aaatcacttc ctgatgatct
    16741 tgtttccact ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat acactgccca
    16801 ataaccgcac aaatatatgc atttgtttgt acaaaaatcg ttggattgtc tccaaaatac
    16861 tcaatcaatt gttggatatt ttgataacaa gcacaatctc tcaaagtatt tgattggttt
    16921 tatctgaatt caaaatctcc ccttccttct atcttagata aaaagaatag gaagactcct
    16981 tcctattctt tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa cacttggttg
    17041 tttttgatct aaaccttttt cttgtgattg tttcatatta ggattatttg ttggagcttc
    17101 tactttctct aatcctttcg cataaatcac taaatctgaa tattcacgat tgccatcttg
    17161 tgctggaata tataccacat gatattctgg atcaattaat ttcacaggat cacgtcgttt
    17221 aaaaccagtc aaatctacat ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc
    17281 tggatttcca aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt
    17341 ttcattatca aatacacgat cagactcttc taatgcgcca aagcgtacgg ctcctaaaca
    17401 agtttcatct aatgcttctg taatgtcata attatttgga aattttaatc ccatcttaca
    17461 aaacctcccc aatttttaca ccagttaaaa agacaccaac atcttgatag ccagttgttc
    17521 gacgctctgt ttgtcccata tcatttgttt cacgactatt cactcgttct ttacgaaaaa
    17581 caaaataagc ttcttcaaat gaaatacgca cctctttttc tggtaatcgt aatcttttat
    17641 cctcttcagc tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa cactctgctt
    17701 gtaattgaac atatcgccca gacacaccat taatttcctt tggtggatta ggttcccgtg
    17761 aacccaataa aaatacttga cctaaaaact ctttcttcat acgcaatgct ttttctgcca
    17821 taaagttttt cctcctctta aaaaccaaaa taaattctaa atatttctac attacaatca
    17881 aacaagcggt taaaaatcct aaccgcttgt tcctcatgac taatctttgg ctttacgtac
    17941 tttataaagt gttataccta aagcaatgac taatactact aatccaagaa ccataagtat
    18001 actacttgtt tgttcacctg tttttggcaa cattggtaca acagtagctg gtttaacccg
    18061 ttgttctggt ttcactggtt gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc
    18121 tttgtttgtt agatctacat tgtgtttcgc cacttcttca aacgtttctt tctcatcttc
    18181 cgttttcgct tggagccatt ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc
    18241 cactaagaat tcaaataaga attccatcat ttctttatcg gccgcaaaga ctttttccgc
    18301 atgagtgcct tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata
    18361 gatttgacca atcgcaagac cgtctacttt gacccattca agcacagttt gatttggcaa
    18421 ggccaagatc tcttttccgc catttttatt cgtcgctttc gtcgtaatgg ttggattact
    18481 gattttgatg gtttggtcat tgtcggtaat atctgcgtga gaagccaata atacgccttc
    18541 tctaaacaga tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat
    18601 aaagtctaga ttgactgtac catccgttgt ttctgccaca aacgttaagg tacttgtgac
    18661 ttcttttcca tctactagca ctggtttttc cgttactttg tccattagaa caccgttgac
    18721 tgtgtatgtt tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt
    18781 cgttaatgga ttaacctctt tcaagccagt gggtttatgt gtagccgttg tacgtacact
    18841 tgggttagtt actcgtaccg tttgcccttt gtcgttgatg tctttatggt ttgctagttc
    18901 ttcatcattt ctaaaaagac tttcaaacac gacgatttca cgaccattta tacctgtcaa
    18961 atcaaaggta aatggtacga tcaccgtttc attttctgct ttggcaataa agccaacact
    19021 cgtttctacc gtatttccat gcacaagcaa gggttcaccg gttgctttat ccattaagat
    19081 ccctttgacc aagtatgctt ttcctggaag aatctcggta atttcaacgg tatcgtttaa
    19141 ggtaaacaat cctgttgggt ctgcgactgt caatccgtct tcaaagacag ccgtggtatg
    19201 aacggttgga ttattcactt tcacactttg ggcttcatcg ttgatatccg catgtgctgc
    19261 aacttcaatg ccatcacgga aaagtttttc aaaaacaacg atcgtttgac ctttcagaac
    19321 actagcatta accacaaatt caaggtcttt tgtgccattg actttgtctg caataaaggc
    19381 caaggtactt gtgatttctt tgccatcttg taaaattggc gcattcgttg ccttgttcat
    19441 caatactcct gaaatggtgt attcttttcc gacaaacaag ttggtataag aaacactgtc
    19501 tttaatgacg atcgtttcta atggatcaac cactttttca ccatcggctt tatttgacgc
    19561 ttgtgtttct agttttggtg gaatcaagta attacgtaca gaaaccacta acgtttcccc
    19621 atcatctggc gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg tatttggttg
    19681 gaaactttct aatggggcat tttccaagaa gtaatagtca tcggcaacta gacctttcac
    19741 taaaatttcc ccgttttgat cagtggtata ttcaccaatt gcttgatttt taccgttata
    19801 caaggtaaag ttcacacctg cgagcggcgt tttcttcttg ttccattctt catacttgaa
    19861 taattttaca cttttccgtg cttgacggtt cgcaaaatgg atcactttga tcggttctgt
    19921 atcattggta atcgagaaca ctaatgggtt ttgattcaaa acgtaattta cgggggcttt
    19981 gatttcttcc aaacgatagc gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa
    20041 gcctttttca ttggttttaa agatatccgt tgtttctgta tcatttggaa tctccattgt
    20101 gacataacga ttggctaaag tatcaaaaat cttgaattct gtattagcaa gtggaatcac
    20161 ttttcctgtt tcttgatcca ctttcaccac tttaagacgt tgttcaatta ctttatcttc
    20221 taaaacataa tggaaagttt gcccattttc agaaattgtg acttcaagtg gttcaaatag
    20281 tttataccct tcttttcctt tggtttcaga aagaatgtac gtatcaaaga caacggtatt
    20341 tttaccaaaa gtagcataac catcattgtc tgtcacaacg gttttcacca ctttgccagt
    20401 tgtttttgac gtcaaagtga tttcaatgcc agctaatttt ggtttgttat ctggattcgt
    20461 tgaatcgact aaatctttat tagcaacttt cactaaatca atggttcctt tgatcacttg
    20521 atcttttgcg gtcgcattga cagtttttcc aggttcaaca acaatttcaa ttgcttcatc
    20581 agaaagcaca taaccttttg gcgctttgat ttcttttaaa acatatttgc caggatcaag
    20641 atctttaatt tctgcctgta ctttgccgtt aatttttttc aaggtaattt ctttaatctt
    20701 tttcccatct gcttgataca aaccatagac cgcattttct aaagaagctg caccttgtgc
    20761 tttcacacca gtttccgcat cttctttaaa cacacgaatg tttccttgtt ttagaatttg
    20821 taaattaata acagacgcat taggttctgt tggatgtaac acaccaattg tttgagctcc
    20881 tggacgacga taggctaatg gcgcaccttg aactttaatt ttattcaatt gaatttttgc
    20941 tttatccggt gcctcttttg tgactgtaat ccatacttca ttgccgtgaa tagatacatt
    21001 gacacctttt ggtttttgaa tgtctttcac atatttcaaa aaatcatttt tattctttac
    21061 tttcaacgtt tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc
    21121 aaagttatta atcaaacgtt gaatttctgc ttttcgacga tcataatcaa cacggatatt
    21181 tttgattggg atttttgtgc ccaaagcttc ccaaatcata aattgtgccg caatgtattg
    21241 ttcatcggat ttgtctttat ttgtgacata tcctaaataa gagatcaagc ttgttttttt
    21301 ccgtacattt gaatttttca aatagttttt caaagcagta gctgtataaa cttgaccttc
    21361 tgcggctgag acaccaggct caatacaaaa cgccacttgt ccattaatct caaaacgaac
    21421 catataattt aaagtgaagt ttagattgtt cggactagaa gtaacaccaa tattttcgcc
    21481 ccaatcaatt ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga
    21541 acgattggtt ggtactgtta ctaacagtgt aggtgtacct gtttttggaa tgttctgctc
    21601 ttttttcttt tgattttcaa tactagccgt agattctttg tttggtttcg ttggttgatt
    21661 ttgactagct gcggttgaag agattgtttt ttcaacgttt tcctcatttt ttccttcttt
    21721 gtcagcagta gccgctggtt gttccactgt ttcttctatt cgtttctttt cttccgcttg
    21781 tttctcagaa gactctggct gcacagaagg cgttgcttcg gcagcttttt ctccttctag
    21841 agtggacgtt tctgaagcag gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt
    21901 accaggacta ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga
    21961 attgaccgtt ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac cagctgggac
    22021 cccggcttct gctggttgga cttctgtcac ttcagaagcg acttgggcaa tggcttccat
    22081 cgtaggttgt atagccgtcg tttcttctgc atgtgcttga gcagttccca ccaagaaaca
    22141 gaattgagaa aaagccacca atgcgagtgc tttcaaccaa ttcattttcg tttttgcata
    22201 cacaagaatt cctccttttc ctgagaattt tatataataa aacaaaacgc cgttaccaaa
    22261 agtgataacg acgtgaactg tctcttctat ttttatctgg cgctcttatc tgaacacatt
    22321 tcatcaatga ttttaccctc ccactataat tacagaacaa aaaaagaacc tgcttcgtgg
    22381 aaatcaactg tccatttccc aattggaata ccagcttttt tggcaacttc accatcacca
    22441 aatctcatgc cataccaatt actaccttca tccttaattt ctttccaacc atatgacata
    22501 gcttcatcta atgaatcaaa aatcatgaca ctattgccaa ttgttacagg atctggctta
    22561 agatcttctg gaagagaaat atcaatcata ccataatgat ccgattcctc agtattattg
    22621 tcattactag ctactggttt ggttgttcta ctggcttatt ttgatacaac aagtactctt
    22681 attcttcttt tacttctccg ttttcaattt cccattcaaa ccactccgca aagtcttgct
    22741 taaaaccatc tactcgatac ctattttgta aataggcatc ttctgacgtc gtaggatcta
    22801 aataggtagg tttattctct ggacgagcat agacacctac ccatgtttcg taatctcctt
    22861 caaaataact atctaccaaa tagccttttt ttctaagaat ctcccgaact tgatttttaa
    22921 ttattgatac ttgttcaaaa ccatcactca ttctatcacc tcaaataact ctagattctc
    22981 ataaatattc ccgacaattt caatcctatc aatgatctct aacaaatcgc tacttatgct
    23041 tccccattcg tataagaatt tcccttccgc aaaataaatt ttccataaac ttcttcatga
    23101 tcgtcccaac caatatcact ctcgaaaaat tccacgttgt tcttatcttt tagctcagta
    23161 aatgccataa gttcaacatg ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt
    23221 acacgtgtgc cttcaatacc taagaaactt tgtcctcgtg tattaaacac taaaactttc
    23281 cgcatttttt gctcattaat taatcacgcg cgaaatctag gaatcacaac ttattcgctt
    23341 cttctttata aataaaaaaa ctccaataat cggaaattac tatcttttcc tcattccttt
    23401 tttaaatctt catacttctt cttatctttg tcctaaccta ataacacaaa ttttgaagaa
    23461 aaactcagta aaacctgatt ctttttttat cttcacaaca accaactttt tcttcagatc
    23521 cttttactag aacttgtata caaaaattgt tgaatgctga tataatttgc ggctacttga
    23581 aaaaacgata gatgtaaaaa gataaataga atcatttttt attctatatc agtctatgat
    23641 tcattgctta tcaatcaccc aacccattcg atgccgtttt ttttagagaa atttaaaaaa
    23701 gaccgtacga atctcgcatt atccgattac ttttccagaa aagatcccca attctgtata
    23761 aataacccta tcttcttgtt ggtttcattt tctcatataa gaattcagtc aatctaccca
    23821 ctatgaccat tactacgtcc aacacaactg acatattaac cacgtaagta ttccctcaca
    23881 gatctccgaa tctccatggt atggattccc atcctgacgc tgcagaggac aacatatttc
    23941 atgtggctct gtaccctttt gatttggaaa cactacaaat caatactcat ctataactta
    24001 tcaagcgaac tttcgctagg cttctagtga ttactaaatt gttggataac atacatagcc
    24061 cattttatct agctgttatt cataacagtt agttcttgta ttacttcttc cgcttgtgtt
    24121 gccattgagc ttgtcaaaaa gttacttaga aaaaagctgc cagtaaagca actttcaaca
    24181 aacccttttt gtttcttat
//
LOCUS       DACDVT010000039        23576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDVT010000039 DACDVT010000000
VERSION     DACDVT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.039"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWL52_001572"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWL52_001572"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9778124.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IWL52_001573"
     CDS             complement(1174..1869)
                     /locus_tag="IWL52_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9778125.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IWL52_001574"
     CDS             complement(1882..2598)
                     /locus_tag="IWL52_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9778126.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IWL52_001575"
     CDS             2928..3533
                     /locus_tag="IWL52_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9778127.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IWL52_001576"
     CDS             3858..4181
                     /locus_tag="IWL52_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9778128.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IWL52_001577"
     CDS             4363..5733
                     /locus_tag="IWL52_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9778129.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRIFQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IWL52_001578"
     CDS             complement(5863..7164)
                     /locus_tag="IWL52_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9778130.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IWL52_001579"
     CDS             complement(7394..9385)
                     /locus_tag="IWL52_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9778131.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IWL52_001580"
     CDS             complement(9529..9795)
                     /locus_tag="IWL52_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9778132.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IWL52_001581"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IWL52_001581"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9778133.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IWL52_001582"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IWL52_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9778134.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IWL52_001583"
     CDS             13139..13702
                     /locus_tag="IWL52_001583"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9778135.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IWL52_001584"
     CDS             complement(13955..14476)
                     /locus_tag="IWL52_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9778136.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IWL52_001585"
     CDS             complement(14479..15321)
                     /locus_tag="IWL52_001585"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9778137.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IWL52_001586"
     CDS             complement(15314..16039)
                     /locus_tag="IWL52_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9778138.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IWL52_001587"
     CDS             16215..16511
                     /locus_tag="IWL52_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778139.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IWL52_001588"
     CDS             17079..18671
                     /locus_tag="IWL52_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9778140.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IWL52_001589"
     CDS             18684..19424
                     /locus_tag="IWL52_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9778141.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IWL52_001590"
     CDS             19592..20941
                     /locus_tag="IWL52_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9778142.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IWL52_001591"
     CDS             complement(21069..21659)
                     /locus_tag="IWL52_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9778143.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IWL52_001592"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IWL52_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9778144.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6754 a         4746 c         4490 g         7586 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatctttc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaaaaa
//
LOCUS       DACDVT010000040        22902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDVT010000040 DACDVT010000000
VERSION     DACDVT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.040"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            337..2502
                     /gene="acm"
                     /locus_tag="IWL52_001593"
     CDS             337..2502
                     /gene="acm"
                     /locus_tag="IWL52_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9778145.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2774..3259)
                     /locus_tag="IWL52_001594"
     CDS             complement(2774..3259)
                     /locus_tag="IWL52_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9778146.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3739..4380
                     /locus_tag="IWL52_001595"
     CDS             3739..4380
                     /locus_tag="IWL52_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9778147.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4399..5034
                     /locus_tag="IWL52_001596"
     CDS             4399..5034
                     /locus_tag="IWL52_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9778148.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5052..5867
                     /locus_tag="IWL52_001597"
     CDS             5052..5867
                     /locus_tag="IWL52_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9778149.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6186..7622
                     /locus_tag="IWL52_001598"
     CDS             6186..7622
                     /locus_tag="IWL52_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9778150.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7811..10258
                     /locus_tag="IWL52_001599"
     CDS             7811..10258
                     /locus_tag="IWL52_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9778151.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10488..11555
                     /gene="rlmN"
                     /locus_tag="IWL52_001600"
     CDS             10488..11555
                     /gene="rlmN"
                     /locus_tag="IWL52_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9778152.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11748..12851)
                     /locus_tag="IWL52_001601"
     CDS             complement(11748..12851)
                     /locus_tag="IWL52_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9778153.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13404..13952
                     /gene="aac(6')"
                     /locus_tag="IWL52_001602"
     CDS             13404..13952
                     /gene="aac(6')"
                     /locus_tag="IWL52_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9778154.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14498..16159
                     /locus_tag="IWL52_001603"
     CDS             14498..16159
                     /locus_tag="IWL52_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9778155.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16229..16576)
                     /locus_tag="IWL52_001604"
     CDS             complement(16229..16576)
                     /locus_tag="IWL52_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9778156.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16660..17601
                     /locus_tag="IWL52_001605"
     CDS             16660..17601
                     /locus_tag="IWL52_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778157.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17602..18645
                     /locus_tag="IWL52_001606"
     CDS             17602..18645
                     /locus_tag="IWL52_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778158.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18651..19721
                     /locus_tag="IWL52_001607"
     CDS             18651..19721
                     /locus_tag="IWL52_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778159.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19711..20664
                     /locus_tag="IWL52_001608"
     CDS             19711..20664
                     /locus_tag="IWL52_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9778160.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20676..21686
                     /locus_tag="IWL52_001609"
     CDS             20676..21686
                     /locus_tag="IWL52_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9778161.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21971..22792)
                     /locus_tag="IWL52_001610"
     CDS             complement(21971..22792)
                     /locus_tag="IWL52_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9778162.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
BASE COUNT         7724 a         3929 c         4753 g         6496 t
ORIGIN      
        1 tagtataaac taataatgtt aattaaaagt gtattttatg aaactatcgt tatttattaa
       61 tgttgcattt ttgtaagtta aaaattggaa atatagattg aaaaaatgaa agcaataagt
      121 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
      181 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      241 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      301 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      361 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      421 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      481 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      541 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      601 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      661 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      721 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      781 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      841 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      901 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      961 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
     1021 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
     1081 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1141 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1201 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1261 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1321 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1381 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1441 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1501 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1561 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1621 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1681 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1741 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1801 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1861 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1921 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1981 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     2041 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     2101 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2161 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2221 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2281 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2341 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2401 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2461 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2521 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2581 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2641 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2701 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2761 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2821 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2881 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2941 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     3001 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     3061 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     3121 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3181 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3241 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3301 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3361 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3421 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3481 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3541 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3601 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3661 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3721 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3781 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3841 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3901 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3961 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     4021 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     4081 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     4141 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4201 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4261 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4321 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4381 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4441 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4501 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4561 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4621 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4681 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4741 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4801 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4861 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4921 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4981 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     5041 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     5101 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     5161 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5221 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5281 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5341 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5401 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5461 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5521 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5581 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5641 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5701 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5761 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5821 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5881 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5941 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     6001 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     6061 tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt
     6121 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     6181 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     6241 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     6301 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     6361 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     6421 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     6481 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     6541 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     6601 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     6661 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     6721 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     6781 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     6841 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     6901 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     6961 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     7021 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     7081 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     7141 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     7201 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     7261 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     7321 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     7381 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     7441 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     7501 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     7561 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     7621 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     7681 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     7741 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     7801 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     7861 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     7921 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     7981 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     8041 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     8101 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     8161 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     8221 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     8281 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     8341 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     8401 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     8461 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     8521 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     8581 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     8641 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     8701 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     8761 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     8821 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     8881 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     8941 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     9001 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     9061 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     9121 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     9181 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     9241 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     9301 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     9361 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     9421 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     9481 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     9541 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     9601 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     9661 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     9721 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     9781 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     9841 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     9901 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     9961 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
    10021 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
    10081 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
    10141 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
    10201 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
    10261 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
    10321 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
    10381 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
    10441 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
    10501 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
    10561 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
    10621 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
    10681 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
    10741 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
    10801 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
    10861 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
    10921 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
    10981 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
    11041 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
    11101 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
    11161 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
    11221 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
    11281 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
    11341 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
    11401 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
    11461 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
    11521 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
    11581 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
    11641 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
    11701 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
    11761 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
    11821 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
    11881 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
    11941 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
    12001 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
    12061 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
    12121 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
    12181 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
    12241 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
    12301 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
    12361 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
    12421 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
    12481 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
    12541 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
    12601 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    12661 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    12721 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    12781 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    12841 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    12901 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    12961 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    13021 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    13081 tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt
    13141 tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac
    13201 aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg
    13261 tttctgtata atttttttat tttataagta agataaataa tgaaagaata gagatactga
    13321 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
    13381 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
    13441 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
    13501 cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
    13561 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
    13621 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
    13681 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
    13741 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
    13801 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
    13861 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
    13921 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
    13981 gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa
    14041 cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct
    14101 tagtttcgag tagtaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt
    14161 ggcttaatac cgagaaactg gcttttgaac accgtttatt cagataaggg actgtgacaa
    14221 gacttttgtc acagtttctt tttgtttatt cttttttatc tttgtatggc aataatagac
    14281 aatataacga aaattattta atggcttttg gaagaccatg gaaaaccttg tacaaagcta
    14341 ttttttctga aaaattcctt gaaacctcac gaaaaaatca ttattttatg aaaaagacaa
    14401 aaaaaagtgt tgatttttta ataattattc attaaactct taaacaactt cagaaaatat
    14461 taattattct gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg
    14521 tttggttgcg gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt cagccacaag
    14581 cagtagttca gcaaaaagcg gttcagcaga tggaggaggc gtatttaact tagtcgtgcc
    14641 tcaagaaatg cctacagctg acttatcagt tgcaacagat acaatcagtt ttacagcatt
    14701 gaataacgtt tatgaaggaa tttaccgtct tgatgaagac agcaaaccac agccggcagg
    14761 agctagcgag ttggcagaag tcagtgaaga cggattaact tacaaaatca aattacgaga
    14821 agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg gattacgttt atggctggca
    14881 acgaacagta gacccagcta cagcttctga atatgcttac ctgtatgcgc cagttgaaaa
    14941 tgcagaagac attacagctg gaaagaaaga taaatcagaa ctagggatca aagcagttgg
    15001 tgactatgaa ttagaaatca aattgacgaa acaaacacca tatttccaat atctgcttgc
    15061 tttcccatca tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc
    15121 atctgcaagt gataaagctg tttataatgg accattcaca ttagaagatt ttgatggacc
    15181 aggaacagat acagaatgga cttataagaa aaatgatcat tactgggaca aagatacagt
    15241 aaaactttct gaaatcaaag tcagtgtagt aaaagaatct tctactgcat tgaacttatt
    15301 caaagatggc caagcagatg acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa
    15361 cgatccagct tatacttctg ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg
    15421 tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa gctatttctt attctatcga
    15481 tcgtgaagct ttagtaaaac aagtactagg tgacggatca gttgtctcta caggattgat
    15541 cccatcagat atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag aagctggaaa
    15601 attagtttct tatgacaaag ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt
    15661 gggcgttgat tctttagaat ttgatttgat ggcttctgat gatgattcat cgaaaaaagt
    15721 aatcgaatat gttcaaaact caatccaaga aaacttggat ggcgtaacag tcaaaccaac
    15781 acctgttcca ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg acgcagtact
    15841 aggcggatgg gctgctgact atgcagaccc aagcagcttt actgatttgt ttgtcacagg
    15901 taattcatac aaccgtggtc gctggagcaa tgcagaatat gacgaagcag ttaaagcagc
    15961 ttcatcaaca gacgctggcg atgaacaagc ccgttgggat gatttccaaa aagcaatcaa
    16021 gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt
    16081 gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc gcttcatggg attacaaatg
    16141 gacatacgtt gaagaataat cgttaatacc gtgagcatga gaaaaaacga ggctgcggca
    16201 aatgtcgcaa cctcgttctc atattcactt atcctaacaa agaaaaaagc atgaaaatag
    16261 cagaaagagc aattagtatt ccggcaatga tcagccaaaa gctgtacata ccgtagctgg
    16321 cagacgagag cgaggaaaat tctggttttt tctttcggtt ccttgttcgt gcaagatgga
    16381 ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa ccgccgataa
    16441 tcagaaacgg caatgaccat aagaatagat cattagataa ttgaatgagt gtaaaggaat
    16501 ggttgatgag attttttatt aaaataacca gaatcagtag aaaagctact gcgtagggga
    16561 tagatttttt tttcatttga ttgctccttt atttttggtt cattatctaa taatgtagcg
    16621 aaacatctgt cagaagtcaa aagctagggg gaaacattag tgaatagtta tatcaaatat
    16681 gtattaaaac gtgtcttttt catgatcatc acgctttggt taattgcaac aatcaccttt
    16741 ttcttgatgc aattgctgcc gggcacgcca tatacgaatc aggaacgttt gagtcctgaa
    16801 acaatcgaga tgctaaataa acaagtcggt ttggataaac cagttatcgt acaatatggg
    16861 atttatcttt ctaatttgct tcaaggagat tttggaattt ccttccaatt taaaaaccag
    16921 ccagtcgcac atttgttagc tggaagaatc ggaccatcct tgcagttagg gttacaggca
    16981 attatctttg gaacagtcct agggacgatt ttaggaacga tctctgccat gaagcaaaat
    17041 acatgggcag atacatcttc tacactcgta gcgatattag gtcgttccat tccaaacttt
    17101 gtatttgctg ttttattaca atatatcttt gcaatcaaat tacaagtttt gccaatcgca
    17161 aaatgggatg gctttgtgta cacaatctta ccaacaattg cgttggccat gtcgccgctt
    17221 gctgactctg ctcggtttat ccgtacggaa atggtggaag tactgcatag tgattatgtg
    17281 gaattagcaa aagcaaaagg attgagccgt tgggaaatcg cctttaaaca tggtcttcga
    17341 aatagtttga ttccattgat gactttatta ggaccgttag ctgtagcatt gatgaccgga
    17401 tcactggtgg tagaaaatat ctttgctatt cctggtatcg gggaacaatt cgttaagtca
    17461 atcacaacaa atgattatcc tacgatcatg gctgtaacga tcctttactc atttatgctg
    17521 atttttgtta ttttgatcgt ggatctattg tacggtctag ttgatccaag aattcgtgtt
    17581 tcagaaggga gccgaggata aatggaaatg ataccaaagc gttatgaaac agttgcagat
    17641 atccctgcgg atgaatttct tccattacaa aaaaataccg aagaagaacg tgaacagatt
    17701 actgcacctt ctttgaattt tatccaagat tcatggcgcc gtttaaagaa aaacaaagct
    17761 gccgttattt caatggtttt actattggtc atcattttga tctctgtgat cacaatcttt
    17821 gtttctcctc atgatccaac tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt
    17881 ccgggaatca atatcgatgg attgaatgga aaagcaatgg ttggtggtga acttgttgat
    17941 aaatatgctc aagcaaacgt cccttcaaac gtaaatttct ttttaggaac agacggtttg
    18001 ggacgtgatg tattaagccg cttatttatg ggaatgagaa tctcgttgct gatcgcattt
    18061 attgctgcat tactagatgt aacgattggt gtagcttacg ggttggtttc tggtttgtta
    18121 ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa tcttatcagg aattccaaac
    18181 ctcgttgtca tgattttgat gttggtcgtt tttgaaccag gaattttttc aatcgtggct
    18241 gctatggcca tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa
    18301 ttaaaagatc aagaatatgt tttagctgga ttgacattag gtgaatcaag aatgaaaatt
    18361 gcattcaaac atattttgcc gaatatctct agtgtcatta ttattcaaat gatgtttagt
    18421 attccatctg ctattttctt cgaagccttt ttaagcttta tcggtttagg tcttacgcct
    18481 ccgtcagctt cattaggaac gatgttgagc gaagggtaca agacattcct gtatctgcct
    18541 tatctattat ggattccggc agtaacttta tcggttatca tgattggctt taacttactt
    18601 gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa
    18661 ttttagaagt gaaagactta gaaatctctt ttgatacgta tgctggaaag gtacgagcga
    18721 ttcgcggtgt caattttcat ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg
    18781 gaagtggaaa atcagtgacg accagaagca tcatgcgctt gctgtcaagc aacgccaata
    18841 tagatgctgg tcaaatcctc tttaaaggta acgatatcgt tggaaaatca gaaagagaga
    18901 tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca agatccaatg acctctttag
    18961 atccgacgat gcccattggg aagcaagtcg ctgagtccct tagaaagcat aataaagttt
    19021 caaaaaaaga gggacaaaaa gctgcattag aattgcttaa attagttgga atacccgaag
    19081 ctgagaaacg tatcaacagt tatccgcatc agttctcagg aggtcagcgt caacggatcg
    19141 tgatcgcgat tgcactggta tgttatccgg aaatcctgat tgctgatgaa cctacaacag
    19201 cgcttgatgt gactattcaa gcacagattt tggagctgct gaaagaaatt cagacaaaaa
    19261 tcgatacatc aattatcttt atcactcatg accttggtgt agtagcgaat gtcgctgatc
    19321 gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg aacatcggaa gaaattttct
    19381 acaacccgca acatccctat acttggggac tgcttggttc aatgccgaca ttagatagtg
    19441 cgaacgatcg attgtatgct attcctggtt ctccaccgga tttattgaac ccgccaaaag
    19501 gagacgcatt ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa caagaaccac
    19561 cattttttga actatcaaaa acacacaagg cagcaacttg gctacttgct ccgcaggcac
    19621 ccaaagtgac accgccagaa gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa
    19681 aacatactta cggtcaagga ggaattgctg atgccaaata aactacttga agttaaagga
    19741 ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc
    19801 tttcacattt atgaaggaga aacattcggt ttagttggag aatctggaag cggaaaatcc
    19861 actactggcc gtacgatcat tcgcttgaat gaacctactg atggtgagat attattcgac
    19921 ggtcaagata taacgaagat caaagataaa aaaggtttga cgaaattccg gcatgatgtt
    19981 cagatgatct ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt cagagatatc
    20041 attgccgaag gaattgatgt taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa
    20101 gtcgatgatc tattgagaac tgttggattg aatccaagcc acggtactcg ttatcctcat
    20161 gaattttcag gtgggcaacg ccagcgtatt ggtattgcac gagcattggc agtaaaaccg
    20221 cgttttatca tttgtgatga accaatttct gcactagatg tgtcgatcca agcccaagtg
    20281 gtcaatttgt tgcaagatct ccaaaaagaa catcaattga cctatctatt tattgcacat
    20341 gatttgtcga tggttaaaca tattagtgac cgaatcggtg taatgcataa cggcttgtta
    20401 ttagagatgg gaacaagtga tgaaatctat aatcacggtg ttcatcctta tacagaaagt
    20461 ttgttatctg ctattcctct accagatcca gaccatgaga gaaaacgcag aagaatcaaa
    20521 tatcaacctg aaccagatga cggacaagta cgtcaacttc gtgaaatcgc accagaacat
    20581 tttgtctatg caactgagca ggaagtccca tattacgcaa aaaaactaaa acgacaaaag
    20641 gaatctcttc tggttgctaa ctaggaggga catagatgct aaaagctttt aaattccgta
    20701 tgtatccgac agaagaacaa aaacagcaat tgattcgtac ttttggatgt gctcgattta
    20761 cctataacca tttgctgaaa aagcggcaga aaagttggca acaaacagga gtagccaatt
    20821 tttcgctcac acccgctaca ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc
    20881 ttgctttagc aaatgcacag cttaatttgg atcgggcttt tcgcaattat ttcaagggac
    20941 gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca
    21001 accaaacccg tacgatctat ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac
    21061 taatcaagat aaatcagcat cggccagttg aaggatcgat tcgctccgct acgatttctg
    21121 ctcgatataa tgaagaattt tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag
    21181 aatctttagc taaatggatc gggatcgcct atcatccgaa gacattgatc caaacatctc
    21241 gacctctaga agtaacttta ccaaaatttc accagacaga agaaaagctg caacatgcac
    21301 agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg ttagatcagg
    21361 caagcaatta tcagaaacaa aagagaaaag taatggatct ttatctgaaa caaaagaacc
    21421 agcgagaaga ttatcttgaa caattatctg ggaaattggt caaacaatat gactacttat
    21481 ttgtagaatc ttttccaaaa gaagaggcac atgctgattt ttctattcac gactggcata
    21541 aattaataac aaaattgcgt tataagtccc aatggtataa taaaaaattt cttttgatca
    21601 ataccgacgg agcagaagaa agcaattccg taagaaagag ccaagtagta gaaaaaatgg
    21661 gcagacattc ggttatcaag ggataaaaaa tgaatgaaac tgataggagc gttagggtta
    21721 gccttgttaa taaaaagtct taatgacttt tgtttctagg aatttttctt ttgaagaaaa
    21781 gctcactaat tatgacaaac caataaaaca atagcagaaa aagttcgtac gagctttttc
    21841 tgctattgtt aattttttat tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa
    21901 acgtaactca caaagagttg tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt
    21961 gctcacaacc ttattcatgg tgccccggct tgagctgata gatttttgaa ggacggccga
    22021 ttcctacagg acgttcaccg atctcatcga aatattggag catagctttc ttaaaattgg
    22081 aatgatcgat cgttttataa tcgatcccta agaatttagc aaatactttt cgagcttccg
    22141 taatcgtgaa atctttacct aaaacttgta gaacttgcgg ctcgtgttcc attttattga
    22201 ctacacggtt gaatgctttc aagatgattt gactatggtc aaaagccaaa gtatctttgc
    22261 cagaagactc tccagtgact aaatcaagcg agatagccac ttctccgcta gataaatgga
    22321 tcttgttact atgacgttct agtgtgaacc agcgtacttc cttggcatca tctccggcga
    22381 ttagcggttc ttctccaata aaggctaagt agctgactgt gacgacccag cctcgcggat
    22441 ctcgattagg cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa ataaccacac
    22501 cagtttcttc ctttgtttct cttaagacgc tttctccagt tgactcatcc ttttggacaa
    22561 agccaccagg caatgcccat gaatttcgat aaggatgccc ttttctttgg atcaacaaaa
    22621 ctttcagctg atcttcttct ttgttatagc acatcagtac gatatcgaca gtcaaagagg
    22681 gcttgtcata ttctggacgt tcctgttttt gataccagtt aagaaattct tcttcacttg
    22741 ctttatgttc ataatataat ttttcttctt ctttagaagc aaaacggttc atctgaccac
    22801 ctccattgac agtaaaagta ctttttacct tattgtagca gactctgaaa aaaaaccaag
    22861 gatttacttg aaaaatctag gttatcattt aaatatgaaa aa
//
LOCUS       DACDVT010000041        22876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDVT010000041 DACDVT010000000
VERSION     DACDVT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.041"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            240..2471
                     /gene="pflB"
                     /locus_tag="IWL52_001611"
     CDS             240..2471
                     /gene="pflB"
                     /locus_tag="IWL52_001611"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9778163.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2715..3476
                     /gene="pflA"
                     /locus_tag="IWL52_001612"
     CDS             2715..3476
                     /gene="pflA"
                     /locus_tag="IWL52_001612"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9778164.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3561..4487
                     /locus_tag="IWL52_001613"
     CDS             3561..4487
                     /locus_tag="IWL52_001613"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9778165.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4669..5562
                     /locus_tag="IWL52_001614"
     CDS             4669..5562
                     /locus_tag="IWL52_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9778166.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5696..5821
                     /locus_tag="IWL52_001615"
     CDS             5696..5821
                     /locus_tag="IWL52_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9778167.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5955..6587)
                     /locus_tag="IWL52_001616"
     CDS             complement(5955..6587)
                     /locus_tag="IWL52_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778168.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6735..7277)
                     /locus_tag="IWL52_001617"
     CDS             complement(6735..7277)
                     /locus_tag="IWL52_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9778169.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7412..7801
                     /locus_tag="IWL52_001618"
     CDS             7412..7801
                     /locus_tag="IWL52_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778170.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7805..8569
                     /locus_tag="IWL52_001619"
     CDS             7805..8569
                     /locus_tag="IWL52_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9778171.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8698..9645
                     /locus_tag="IWL52_001620"
     CDS             8698..9645
                     /locus_tag="IWL52_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9778172.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9775..10515
                     /locus_tag="IWL52_001621"
     CDS             9775..10515
                     /locus_tag="IWL52_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778173.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10806..11474)
                     /locus_tag="IWL52_001622"
     CDS             complement(10806..11474)
                     /locus_tag="IWL52_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9778174.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            11608..11895
                     /locus_tag="IWL52_001623"
     CDS             11608..11895
                     /locus_tag="IWL52_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778175.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            12311..13849
                     /locus_tag="IWL52_001624"
     CDS             12311..13849
                     /locus_tag="IWL52_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9778176.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            13846..14811
                     /locus_tag="IWL52_001625"
     CDS             13846..14811
                     /locus_tag="IWL52_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778177.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            14798..16120
                     /locus_tag="IWL52_001626"
     CDS             14798..16120
                     /locus_tag="IWL52_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9778178.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      16183..16287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16361..17104
                     /locus_tag="IWL52_001627"
     CDS             16361..17104
                     /locus_tag="IWL52_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778179.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            17119..18111
                     /locus_tag="IWL52_001628"
     CDS             17119..18111
                     /locus_tag="IWL52_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9778180.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            18123..18860
                     /locus_tag="IWL52_001629"
     CDS             18123..18860
                     /locus_tag="IWL52_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778181.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            18850..19572
                     /locus_tag="IWL52_001630"
     CDS             18850..19572
                     /locus_tag="IWL52_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9778182.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(19656..20279)
                     /locus_tag="IWL52_001631"
     CDS             complement(19656..20279)
                     /locus_tag="IWL52_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9778183.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            20674..21366
                     /locus_tag="IWL52_001632"
     CDS             20674..21366
                     /locus_tag="IWL52_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9778184.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            21573..21863
                     /locus_tag="IWL52_001633"
     CDS             21573..21863
                     /locus_tag="IWL52_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778185.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         7890 a         3800 c         4616 g         6570 t
ORIGIN      
        1 taatacaggt ggcgtctctt tttcattcat ttttaataaa tccttatacg tttgcaaaaa
       61 aagatgaaag tgttcacaat gcttaaatgt tggtatgtca acgttgattt aaatatatgt
      121 aaataaaaca taaaaattgt taaataaaaa acaagaaaac ccttgcaaag atagggtgct
      181 gtgttatact aatcacgtaa aaactgttac ataaaacaga aatttaggag gtccatacaa
      241 tggagcaatg gaatggattt aaaggtacca aatggaaaga aggcgtagac actagagact
      301 ttattcaaaa taactacact gaatatcgcg gtgatgacag ctttttggaa ggcccaacag
      361 aagcaacaga aaaattatgg gatgaattgc aaaagttaaa tgatatccaa ttcgagcgta
      421 acggtgtcta tgatatggac acagaaatcg tatcaactat tacttcacac gatgcaggct
      481 atttggataa agatttggaa aaaatcgtcg gtctacagac agacaaacct ttgaaacaag
      541 cattcatgcc gttcggcgga atcaatatgg ctaacaatgc gctaaaaagc aatggctata
      601 caccaaacga tgaattgaca catattttca ctgactggcg caaaacacat aatcaaggtg
      661 tatttgacgc ttatactgct gaaatgcgtg ctgctcgtaa aaacaaaatc attacagggc
      721 ttccagacgc ttacggtcgc ggacgtatca tcggtgacta tcgtcgtgtg gcattgtatg
      781 gaatcgacta tttgatggaa caaaagaaaa aagatcatga tatgactggt tatcatacaa
      841 tgagtgaaga agtgattcgt ttacgtgaag aaatcactga acaatatcgt gcattagcgc
      901 aaatgaaaga aatggctgcg aaatatggct atgatatttc taaaccagct gcaaatgcaa
      961 aagaagctgt ccaatggcta tacttcggat atttagcagc aatcaaatca caaaatggtg
     1021 ctgcgatgtc aatcggacgt atctcagcat tccttgatat ctatatccaa cgtgatttgg
     1081 ataatggagt aatcacagaa aaagaagcac aagaattgat cgaccactta gtaatgaaat
     1141 tacgtatggt caaattcgcg cgtacacctg aatataacca attattctca ggattcccta
     1201 tctgggctac tttatccatt gcaggtatgg gattagatgg tcgttcatta gttacaaaga
     1261 atgatttccg tctgcttcat acattaacaa atatgggacc ttcaccagaa ccaaacttga
     1321 ctgttttgta ttctgaacac cttccagaag gattcagaac atatgctagc agaatcgcta
     1381 tcgaaagttc ttcgattcaa ttcgaaaatg ataatcagct tcgtgaacaa tggggttctg
     1441 atgattgtgc gattgcttgc tgtgtatcag gaacagttgt cggaaaagac atgcaattct
     1501 ttggcgctcg tgctaacttg gctaaaacac tattatatgc catcaatggc ggagtcgatg
     1561 aagtgactgg cgcccaagtt ggaccagaat atcgtccaat cacatcagaa atactagact
     1621 ttgaagaagt gaaacataac ttcatgaata tgatggattg gttagcagaa ctatatgtca
     1681 atacgttgaa catcattcat tatatgcatg ataaatattc ttatgaagca gctcagctgg
     1741 ctttgatgga ttctgacttg caacgcacat ttgctacagg tattgctggg atctctcatg
     1801 cggcagacag cttgtcagcg atcaaatatg caaaagtaaa agcaatccgt aatgaaaaag
     1861 gaatagcagt tgattttgaa gtggatggcg acttccctaa atacggaaat gatgatgacc
     1921 gtgcagatga attagtttca tggatcttgg aatacttcat gggacaaatc aaacgtcata
     1981 aaacttaccg caactcaaaa ccaacaactt cattattgac gattacttca aatgttgttt
     2041 acggtaaaaa tacaggaaat actcctgatg gccgtgaagc tggaaaacca cttgcgccag
     2101 gagcaaatcc aagttacaat gcggaacaaa gtgggttatt agctagcctg aactcaacag
     2161 ctaaaatgaa ttacggctgt gcattggatg gaatttcaaa tacccaaaca atcaatcctt
     2221 caggtcttgg aaaagaacaa gatacaagaa tcgaaaactt gcgtaatgtc ttagatggat
     2281 acttctcaaa aggagcttat catttgaatg tcaatgtatt cagtaatgat ttattgcgtg
     2341 atgcaatgga aaatccaatg aaatatccga acttgacgat ccgggtatct ggttatgcag
     2401 ttaaattccg tgacttgaca agagaacaac aattagatgt cttgatgaga acttcacacg
     2461 acagaatgta aaaagttcgt gaaaaaagtt tttcgactta gcgtcgaaag gacgttgaat
     2521 agccatcgca tcaaaatcga ataatcagta tgcttatttg aaagaaacga tttacagggg
     2581 caggacttgt atgttccgtc cctgtaaatt ttacacataa cgaagcggca attgcgaaaa
     2641 attttgttca gaaagcttcg cttcgacagc taacttgtac ttaaaatgaa atcaaagaaa
     2701 agatgtgaac ttaaatggaa gaaaaaacaa tcggctatgt tcattctatc gaaactttcg
     2761 gttcagtaga cggacctgga ctgcgctttg tcgtgtttat gcaaggatgc cgcatgcgtt
     2821 gccaattttg tcacaatcca gatacttgga atattggcgg agggaaagaa tacacagcag
     2881 atgaactttt agataaagca gaacgcttca gaccttactg gggagataaa ggtggtatta
     2941 ctgttagtgg tggagaacct ctattgcaga tcgatttttt gatcgaacta tttaaaaaag
     3001 caaaagagcg gaagatgcat actacattgg atacatgcgg aaaacctttt acgtatgaag
     3061 aacctttctt ttcacgtttt caagaattga tgaagtatac agatttactg ttatttgaca
     3121 tcaaacacat cgataatgaa gagcataaaa aactaacgca ttgggacaat gaaaatattt
     3181 tagaaatggc tcaatatttg tcaaaaatca acaaaccggt ttggattcgc catgtactag
     3241 ttcctgagcg aagtgattat gatgaatatt tgatccgact agacaacttt attcaaacct
     3301 tgtcaaatgt agacagagta gagattcttc cttatcatac gatgggaaaa tacaagtggg
     3361 aaacacttgg tttgaaatat ccattagaag ggatcgaacc acctacaaaa gaacgtgtcg
     3421 aaaatgctaa acgattgctt catacagaag agtatacaaa atatttaacg cgataggtcg
     3481 catcggaaag aagataagta ttgcttcttt ccgttttttt gctattatta aataggagaa
     3541 atcacaaagg agcgaatgac atgtcaaaaa ttttagtttt tggtcaccaa aatccagata
     3601 cagatgcaat cggggcagcc attgcgtttt cacacttgca aaatgaatta ggtaaagaaa
     3661 cagaagcagt tgctctaggt acacctaatg aagaaacaca atacgcatta gaccactttc
     3721 agctatctgc accacgagta gtagagtcag ctaaagcaga aaatgcagat gtcatgcttg
     3781 tggatcataa tgaattccaa caaagtattt cagatatcag cgaagtaaat attttagctg
     3841 tagtagacca ccatcgtatt gcaaactttg aaacagctaa tccattgtac tatcgtgcag
     3901 aaccagttgg atgtacaagt acgattattt taaaactata taaagaaaac caagtaacaa
     3961 ttcctaaaga aatcgcagga atcatgctct cagctatcat ttcagataca ttgttgttca
     4021 aatcaccaac atgtacgcct gaagatgaag cagctgcaaa agaacttgca gcaatcgctg
     4081 aagtcgatct aaacacttat ggacttgaaa tgttgaaagc aggaacaaat ctaagtgata
     4141 aatcagctgc ggttcttctt gatttggatg caaaaagttt cccaatgggc agttccaacg
     4201 ttcgtatcgc tcaagtcaat acagtggacc ttaatgaagt gatggatcga caagcagagc
     4261 tagaagcagc aatggaagat gaaatgaaag caaatggcta cgatctgttc gtattagttg
     4321 tcacaaacat cttagacagt gattcagaat tacttgttgc tggtaaagga aaagaaaaag
     4381 tagaagaagc tttccaaaca actttgacca acaaccgtgc tttgcttaaa ggtgtcgttt
     4441 cacgtaaaaa acaagtcgta cctcaattga cggctgtatt tagctaatca aatggtagta
     4501 tgaagcattc aaaatgaatg gataagcgat tgatataaaa agaaacatag aacatactaa
     4561 aggccgaaga aatggaggag aataacagtc aattatgact cttgttcttc ctccttttta
     4621 cgtaaaagcc ttttaagtaa aataaaacgg atagaaaaga gaacatatat gaactattat
     4681 tcaaaaaata aggaaaactt ttatcaaaaa ctgaccgatg atccgctttt ttcttcattg
     4741 accgattatc tatatgaaca tcgagaacaa gaaacgattc taagagaatt gaaaaaagaa
     4801 ttttcgcaaa ataaattttc ccattttctt gatttattga tcgatgcagg attaatcaag
     4861 agggaagaac gaagatatca tttgaacttt ccaatatttg atcctaaaga ttacttgcaa
     4921 caagccactt cggctgctga aaccatagct gaacaattga agagactctc tgttgatgaa
     4981 caaaaattag cgatgggaga agttatttgg gcgtattgtt tcgaagatga acggaaagaa
     5041 gcgtattttt atggagtaag aaacagccga gagacagaat tgctgaggac aactgcagga
     5101 aatgagaagt accgttttat cacactttcc tctatagaac acttcccgct tactttggct
     5161 aattactttt ttgtacagaa gaatcagctt cctgtaacga aggcattcaa agaattagcc
     5221 gaacttattg gagacgtaaa tgaatcgtac ttttttgatc aaatagaagt tatcgttgac
     5281 aggatacgta agaataaata taaaaatcgc cggcctagta ttttccatca gtctctactt
     5341 gtgacgaata ctataaagga agaagaatca ttcacattag aattacctat tgtcgaaaaa
     5401 aacaattttg agattgaatt accaacattg gatccttcat tgacaatgga ggaaatggcc
     5461 tttcttaaac gacaaatctt ttctgagctg agcaagaaat ttataccgca tgcattttct
     5521 tatatcaaag aatatggaac agttttagtg agtaaaacct aagagagcag taagcaaaaa
     5581 ttatactgac tcttgcagga ggagcctttc tcttccttta tggttaaaag aattatttga
     5641 gtattttgcc tatctaccac ctattagatt cactacatgg tctggaaaca aaggaatgca
     5701 aaaaaatggt gagaaatgtg gaatgacaaa ggaagtagtg ataagaaaag ttagattttt
     5761 aaataatcaa tattatgatt cagtcaagta tggcatttta tgggaagaac tagctgatta
     5821 gattaggaag tccatttgat aatccagaat cactaagaaa cgaatggcga gtcattaata
     5881 atttatccaa tcttgggtta ttcttttaga ttgaagtgtt taaaactaaa aaagcattta
     5941 tgaagtcgta atagtcaggc ttcaaaatgc tttttatttt gtttgttttt tctttgccac
     6001 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
     6061 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
     6121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
     6181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
     6241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
     6301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
     6361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
     6421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
     6481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
     6541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
     6601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
     6661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
     6721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
     6781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
     6841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
     6901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
     6961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     7021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     7081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     7141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     7201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     7261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     7321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     7381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     7441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     7501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     7561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     7621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     7681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     7741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     7801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     7861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     7921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     7981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     8041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     8101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     8161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     8221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     8281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     8341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     8401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     8461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     8521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     8581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     8641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     8701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     8761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     8821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     8881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     8941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     9001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     9061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     9121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     9181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     9241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     9301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     9361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     9421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     9481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     9541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     9601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     9661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     9721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     9781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     9841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     9901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     9961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
    10021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
    10081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
    10141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
    10201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
    10261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
    10321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
    10381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
    10441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
    10501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
    10561 gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt
    10621 gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa
    10681 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
    10741 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
    10801 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
    10861 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
    10921 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc
    10981 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
    11041 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
    11101 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
    11161 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
    11221 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
    11281 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
    11341 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
    11401 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
    11461 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
    11521 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
    11581 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
    11641 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
    11701 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
    11761 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
    11821 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
    11881 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
    11941 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
    12001 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
    12061 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
    12121 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
    12181 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
    12241 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
    12301 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
    12361 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
    12421 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
    12481 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
    12541 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
    12601 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
    12661 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
    12721 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
    12781 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
    12841 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
    12901 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
    12961 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
    13021 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
    13081 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
    13141 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
    13201 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
    13261 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
    13321 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
    13381 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
    13441 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
    13501 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
    13561 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
    13621 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
    13681 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
    13741 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
    13801 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
    13861 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
    13921 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
    13981 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
    14041 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
    14101 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
    14161 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
    14221 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
    14281 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
    14341 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
    14401 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
    14461 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
    14521 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
    14581 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
    14641 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
    14701 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
    14761 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
    14821 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
    14881 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
    14941 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
    15001 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
    15061 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
    15121 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
    15181 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
    15241 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
    15301 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
    15361 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
    15421 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
    15481 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
    15541 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
    15601 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
    15661 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
    15721 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
    15781 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
    15841 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
    15901 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
    15961 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
    16021 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
    16081 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
    16141 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
    16201 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
    16261 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
    16321 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
    16381 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
    16441 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
    16501 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
    16561 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
    16621 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
    16681 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
    16741 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
    16801 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
    16861 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
    16921 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
    16981 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
    17041 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
    17101 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
    17161 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
    17221 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
    17281 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
    17341 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
    17401 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
    17461 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
    17521 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
    17581 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
    17641 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
    17701 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
    17761 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
    17821 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
    17881 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
    17941 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
    18001 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
    18061 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
    18121 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
    18181 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
    18241 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
    18301 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
    18361 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
    18421 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
    18481 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
    18541 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
    18601 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
    18661 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
    18721 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
    18781 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
    18841 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
    18901 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
    18961 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
    19021 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
    19081 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
    19141 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
    19201 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
    19261 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
    19321 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
    19381 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
    19441 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
    19501 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
    19561 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
    19621 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
    19681 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    19741 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
    19801 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    19861 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    19921 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
    19981 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
    20041 ccttctgttg ggattgttaa ttgttgtcct gagtaaatca ggttaatatc agaaattgaa
    20101 tttgattctg caatggcatt gatcaaggag ttatcaccaa cgtatttttg agaaatcgtt
    20161 gatagagtat cacctgattg aacggtataa gcttcgtccg catgagcagt cgttcccatg
    20221 aagaatgcag cgccggcagc caaagttgtt ccaaaaagta aagttttaag tgatgtcatg
    20281 aaaaatctcc tttattcgtt aaaaagtttt atttgttgtg ttcaacgagg aatactttaa
    20341 cacgtgaaaa tatttcatag gtaaaagaag catgacaatt tatgacaaat ccgcgttttt
    20401 tgtaataaat acagtaatct atgaaatgaa aagggttacg tttaataaaa cttgcttttt
    20461 tgtttttgtt caccataaat atttatttaa gaaagtatta acctgcattt ttctctataa
    20521 aaaagatttt ttaaatagat aaaaaaggag gattaaatga gtatttgata gtatttatat
    20581 taaaattaca tatgtaattt aaaaacgctt gacattgtgt ttgagatcac ttacttttag
    20641 catgtaaaag tattggatta aggagctatg caaatgaaaa agaaatttat tactggtggg
    20701 attgtcgttg catttctatg tctaattggc tacacaggca cgcggtacgc aaatgaaatt
    20761 attttgtggg gtggagagac agatattgat acaatcaatg agaatctgac acagcttgat
    20821 catacattga cagataaaga gcaagcgatt acagatctta ccgctcgact agcacaaaaa
    20881 gaacagacat tgcaagattc aaaggcagat gtccaagaat accaaacaaa agtaaatgaa
    20941 ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa acttagaaag tcaattagtc
    21001 agcaaggatg cacaactaca aagcaaacaa aatgaaatca atgtcaaact agatgagatc
    21061 aaccaaaaaa atgatgaaat caatcatctg aatgcagatt ggacagcaag actacaagaa
    21121 gcacaagcaa aactttcaga attacaaaat actgtaaatg gtttgaatca acaaatcgca
    21181 acactgacac aagaaaaaac aaatgtagaa actcagttaa cagatacaca aaagaaatta
    21241 gacgaagcaa ctgctgaaaa ctcaagcttg aaacaatata ttcaaaagtt agaaaaagca
    21301 aaagatgaag tggaagatac agctgataaa tctcagcaaa ttgttgaaga acatttgcgt
    21361 aaataatctt tcttaacaag taaacctaaa ttagtaaaga agcaacagta atctatactg
    21421 ctgcctcttt ttttagtaca tgtccttcat cttcgcagga agtgccaata tgattttaag
    21481 aaaggtaata gacaaaaagt caaaaagata aaacagagtt tcttctttac aaagggaatg
    21541 tatgttcgta taatggtatt gaggtgaagg ttatggagaa aaaatatgga acagtagata
    21601 agatccgtac tttgcaagtt agtgccagac cgttgatccg tttcagttta aatggggtca
    21661 attgtttgat tgcttcccat agtttgaact tcctagctga agtagacgaa gggatgaagt
    21721 tagtcgttac aggttattat aataatagaa agcaatttgt tgtgagatcc tatcatgtgt
    21781 tagggaaacc caaaattgtg gtagaatatg agaaaagtct atacccaaga aaaaaggtcc
    21841 gacaagatga aagaataaac tagaaaacaa gcggctgaga caaaaatgtc ctagccgctt
    21901 gttttggaaa gatggatgca gacgatggga tttgaaccca cacgagcata cgctcacaag
    21961 aacctcaatc tatatataac tacatgtatt taagtatttg tgatgaatca ctaaactttc
    22021 caaactcatt tgagggatat tgcttaaaga ttgaacagat caattcgaag ccctattagt
    22081 attcgctcaa tggggggatc agaggccata aaaagagact agtcattact agtctcaggt
    22141 agtggaattg taggatttta aacgttcctg gagagtaccg gtatgtaact cgaagaggtg
    22201 attatcatag tcgtaaaagt aaattgaaga agcctcgcct ttcacacgag aacgtcctgg
    22261 ttcgacagtt aaacctaaca tttggatttt tgagacaaac gaatctatat cttgttcgtc
    22321 aattttaaaa gcaatatgat tgtatgtctt tggcagttta ttcgacgaat tctgcattac
    22381 tgcaatccat aggtccttaa ctaagaaaaa tttttctgga tataatgaaa atttttttgt
    22441 tgactatcgt agatttcttt tgcatcaaat aaggtattta acaattaagt atttaaaaga
    22501 taacttttaa gttatcttta agtaatgctt aaagctatgt tatactctat taggaaatat
    22561 aagaaggagt ataaaaatga acaaacataa tagaaaaata acgaataaca aagaactgat
    22621 cttgttacta atagggattt ggggtatctt tttactcttt aatatgtata tgccgactat
    22681 gagagcggat gacgtagttt atgcaagtag attagatgaa cttggctatt tgggtgcttc
    22741 gatagaacat tataagacat ggagttcaag aattattata gaactattct taatgttctt
    22801 ttctaaacac tttatgctat ggaaattatt aaattctacg attatgttag gaattgtagt
    22861 attactttgc aagtat
//
LOCUS       DACDVT010000042        22823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDVT010000042 DACDVT010000000
VERSION     DACDVT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.042"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..637)
                     /locus_tag="IWL52_001634"
     CDS             complement(<1..637)
                     /locus_tag="IWL52_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9778186.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQ"
     gene            complement(639..1403)
                     /locus_tag="IWL52_001635"
     CDS             complement(639..1403)
                     /locus_tag="IWL52_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9778187.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1411..2088)
                     /locus_tag="IWL52_001636"
     CDS             complement(1411..2088)
                     /locus_tag="IWL52_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9778188.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2125..3510)
                     /locus_tag="IWL52_001637"
     CDS             complement(2125..3510)
                     /locus_tag="IWL52_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9778189.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3720..3804)
                     /gene="ffs"
                     /locus_tag="IWL52_001638"
     ncRNA           complement(3720..3804)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWL52_001638"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3827..4342)
                     /gene="tadA"
                     /locus_tag="IWL52_001639"
     CDS             complement(3827..4342)
                     /gene="tadA"
                     /locus_tag="IWL52_001639"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9778190.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4449..5306
                     /locus_tag="IWL52_001640"
     CDS             4449..5306
                     /locus_tag="IWL52_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9778191.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5387..5722)
                     /locus_tag="IWL52_001641"
     CDS             complement(5387..5722)
                     /locus_tag="IWL52_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778192.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5789..7159)
                     /locus_tag="IWL52_001642"
     CDS             complement(5789..7159)
                     /locus_tag="IWL52_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9778193.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7237..7773)
                     /locus_tag="IWL52_001643"
     CDS             complement(7237..7773)
                     /locus_tag="IWL52_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9778194.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8082..9563
                     /locus_tag="IWL52_001644"
     CDS             8082..9563
                     /locus_tag="IWL52_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9778195.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(9666..10373)
                     /locus_tag="IWL52_001645"
     CDS             complement(9666..10373)
                     /locus_tag="IWL52_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9778196.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(10452..10790)
                     /locus_tag="IWL52_001646"
     CDS             complement(10452..10790)
                     /locus_tag="IWL52_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9778197.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(10901..11863)
                     /locus_tag="IWL52_001647"
     CDS             complement(10901..11863)
                     /locus_tag="IWL52_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9778198.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(11878..13209)
                     /locus_tag="IWL52_001648"
     CDS             complement(11878..13209)
                     /locus_tag="IWL52_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9778199.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(13217..13897)
                     /locus_tag="IWL52_001649"
     CDS             complement(13217..13897)
                     /locus_tag="IWL52_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9778200.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(14116..14595)
                     /locus_tag="IWL52_001650"
     CDS             complement(14116..14595)
                     /locus_tag="IWL52_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9778201.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(14722..16401)
                     /locus_tag="IWL52_001651"
     CDS             complement(14722..16401)
                     /locus_tag="IWL52_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9778202.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            16949..18451
                     /locus_tag="IWL52_001652"
     CDS             16949..18451
                     /locus_tag="IWL52_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778203.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            18464..19126
                     /locus_tag="IWL52_001653"
     CDS             18464..19126
                     /locus_tag="IWL52_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778204.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(19227..19430)
                     /locus_tag="IWL52_001654"
     CDS             complement(19227..19430)
                     /locus_tag="IWL52_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9778205.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(19541..20725)
                     /gene="iadA"
                     /locus_tag="IWL52_001655"
     CDS             complement(19541..20725)
                     /gene="iadA"
                     /locus_tag="IWL52_001655"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9778206.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(20882..22621)
                     /gene="yfcC"
                     /locus_tag="IWL52_001656"
     CDS             complement(20882..22621)
                     /gene="yfcC"
                     /locus_tag="IWL52_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9778207.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         6689 a         4864 c         3827 g         7443 t
ORIGIN      
        1 tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc aaaaaataat tctaataata
       61 caaaaaataa gataaagcta tgcatgaaaa attcttctgg aagtacattg atgattggat
      121 cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa
      181 aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac aactggcagt aacatgccga
      241 tttgaaacgg acgaatcaac cgccaaagcc gatcattctt ttgcaaatat ttgataaaca
      301 aaatactcgg gatcaaagtg atgaaaaaca cagcataatc cactaaaaac aaaattttca
      361 cttcataaaa gtgatgggct ccgctagcag aaactgggaa atctggcaag ctgaggatgg
      421 ttttgaaggg attattgaga taattcatta ggtgatcaaa atttttcagt aacgtctctt
      481 gatctaaaga agtataatcc aaaatttgca aatgtccgat atcaaatacg tatagtggtc
      541 gaaagttgat cgtcacacca actgccagac tgatcagtgt cagaaatagg ctgaacaatc
      601 cagcatattc catccactgc catctcgaat ttttcatatt agaagctcca ttcatccagt
      661 gattgtttca cataggtagc tgggataggc aacccaggta catcttcttc ttttgtaaaa
      721 cctgataaaa cgagcaaagt atctatatta ttgcgtatcc cagcttggat atctgtttca
      781 tagttatctc caaccatgat tacttcttct ttttgcagcc ctaatacttc aacggcttta
      841 tccataatga tggcttctgg ttttccgatg tagatcggcg gcgtttgggt tgctgtctct
      901 acaaaagaga tgactgaccc tgctccagga agaaggccgc gttcagtagg tatattctta
      961 tctgggttcg ttccaatgaa agtagcacct ttttgaatag caagtgttgc tttaactact
     1021 ttttcatagg tcagataatt atctaagcct accactacat agtcaggagc ctcttcttcc
     1081 catgtaaatc cggcagctaa aatcaagtca atcaatcctg cttctccgat aacatagact
     1141 tttttccctt ttccatctgc tttcataaaa tcgattgttg ccaaagtagc tgtgtagact
     1201 gtctctggag ctacatgaat gtcaaactca tctgctagac gattagctac tgtttctggt
     1261 gacttagtcg tgttattggt aacaaacaag aaaggtaatt ttcttttttg caattcttcc
     1321 acaaatcgtt taccggccgg tatgggttgt gtcccacgat aaatcgttcc gtctaaatca
     1381 attaaatatc ctttatagtc cattcatcca ctactcttcc ctttttctga tcgtaaatgc
     1441 tgtttttgtt ttttctactt gtttaggttg ttcttgtttt tttacttttt cagaggtttt
     1501 tgctgactct gtttttcgat ttttaataac cggttttttg cgattagatg cgactggtcc
     1561 ttttttctca tcgatatacg cacgattatg gttttttttc ttttttcggc gctgagttgt
     1621 cttttctttt ttaccgccga ttctctcgat gacaaaatac gcacaaccaa aattgcaata
     1681 ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct ggatgcgccc gtcgattatc
     1741 cgcttggaaa aatcctttta ggcgtagttg ttcataaccc caatctccta cgatataatc
     1801 atatcgggct agtacatcac tgtaacgttc tccaagcttt tctgcattaa atccttcacg
     1861 ataatcggac acaagccgat aatgtctttc acctatcaaa aaattactgt catctaacag
     1921 tgtcactagc tcgcctttga ctttttctgc tttaggctcc tcaacgattt cttccaaagc
     1981 agctgtcagt tcttcggtga ctgttgtttc ctgttcagct gatgattttt tcttagcaat
     2041 ttctgtttta tgctcaacgt tctgctgttc attttgtttt gcagacatgc gttcaccacc
     2101 tatctttttt attatacctt gtttttattt gattgggtag tgagctttca agtaatctcc
     2161 taccacactt cggataaatt cagggaacaa aaattcattt tttccagcaa tctcaatcgt
     2221 tgggaaaaat ggaacaaaca tcaaatgatc tacggtagta aaagtataat ttttattcag
     2281 atcgattggc ttgttcttcc aaaagacttg atggttaata gaatcatatg tgattccatt
     2341 atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag gatagttacg
     2401 taaaaagtta cgattttttt ctatttctaa aactaagcgg atcagatcat ttccacccaa
     2461 tgtgacattt aacaagtgca tcggatgcgg caacgcagtg tgaagctgat cctgattgac
     2521 ccgccctttt ggaatttcag ttaaaaacag accactgttt aatattgctg cttccgtctt
     2581 tcctcgttct ttcaccgcct caaaggctac ttggataaac gaatgctcat tgaagatatc
     2641 tagactcaga gaaaatggca aatctgctac ttctttttct tttaataatt catgcccacg
     2701 ttctagataa ctatttattt cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg
     2761 gatggctttg gctgaatgct taaggatttt tttctcttca tcgatggtta aatggacttc
     2821 accgacatac tgcccgtact ttccagcggc tgctatttgg acatcattga ttacgtgacc
     2881 ttctctgaat aaatgatggg tgtgcgatcc taaaataaca tcaattgctg gtatttcttt
     2941 tgcaatttgg aaatcatcct gaatcccgag atgactcatc aaaactaaaa catccacttg
     3001 tgaacgtaaa tcttcgacta attctggcaa aatatcataa ggattccgta tgtcccagcc
     3061 attcggatta taagtaagcg gaaatggagc agtaaatgca attagtccga ttttcgtctg
     3121 gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc ggtggctgga gcgtattttt
     3181 atcgaataag ttatccagga tgatatcaaa ctcagcatga tcgtataaat gattcaactc
     3241 ttctttggaa tttcctacgc cttcattgtt tcctatcgtc gctgcatcat aaccaacttc
     3301 attcatcagc aagatatttt cctgtccatt cgttgcttct gacaatggat gccaacgatc
     3361 cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg
     3421 tttacgttga tccaaaaacc ttctgatcct tggccaattt tctaaatgag aatgcagatc
     3481 attcgtatgt aaaatcacta ttttttccat gtaccctctc gcctctcttt atctttatca
     3541 taccataggc agctttttct tccattgaaa caagctctat ttcagagttt ttcctgcctt
     3601 gaagcaaatt gtataagtac aatattcgat aaatcagtct ggctaaaaaa tcaggaacat
     3661 tcatttttac agtactataa aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat
     3721 tataatcaga cacatggcgg aacctgctta gtgctgcttc cttccagacc tgacacgctt
     3781 cacaagccca ccattgtcct agcctttcgg caaagaatag tataccttat ttttcattcg
     3841 ttgaccagtc tttttttaat ttcttttttc gctggcggag ctttcggaaa aagtcagtca
     3901 aaatcatccc acattcgtct tctaaaatgc caccttctac atatgcttgg tggttaaacc
     3961 gctcgtcctc caaaagattc atcaaggtac cggctgttcc gccttttgga tcatacgcgc
     4021 cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct
     4081 ctaaggtaac aaaaagtgca gcatcttcca agcgccagct tcccactttt tcacaggctt
     4141 ccttgattgc aagcatttct gcatgtgtag tagcatcttg cgtggtttct ctaaggttat
     4201 atcctcttcc aatcactttt ccatccaata caaccactgc tccaatcgga acttcatgaa
     4261 gcatttctgc tttctttgct tctgccaatg caaggcgcat ccatttttct ttttctactg
     4321 catcaagatt tgtatttacc aattaccctt ccctgccttt gttttttctt gttttacttt
     4381 agcatatgat aaaaaggctg taactaactt ttttttctat ggtaaaataa atacaagagg
     4441 tgaacaagat gaaaatggat aaattacttg ctctttaccc acaagcacag aaaaaaaact
     4501 ctccagcatc agaagcggat atcctttctt ttgcaattga tgatgctttt atctggatca
     4561 aacaagattc attatctcaa caagaaacca gccttttgaa agcgttattc cctgtaatca
     4621 acgatcaaaa aaaacaccca tggtatcact atctttttaa agatgcttct tgtgttcaag
     4681 aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag
     4741 aatggcaaga tactattttg gaattatttc gctattctgc tgatttcttt ttttatacag
     4801 acacagatgc gctcttagtc gaacaaataa accgtgatta tcttgatgct actgaaatca
     4861 acggtatttt tctatcttta gatgctgatt ttgatacgac aacgtctgta ttcgttggtt
     4921 ctttccattc acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt
     4981 taacagagca aaacaatctt tatactcaca gccgtacttt ttctatgcaa gatgtggctt
     5041 tacattacta tacaaaagat actgtaaaag atagtaagtt gatcaaaagt tacatagaac
     5101 tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg
     5161 tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa
     5221 aatttcaaga acagtctggt ttcaatctaa aaaaagccaa cgatttactg ttttgctatc
     5281 ttttattgct tcatgaacat aattaaagat agattaaagg ggttgtgaca ataattttgt
     5341 cacaacccct tatttcgaat gaatagtgtt caaaagctag tttctcttac atcatttttt
     5401 caacaaaatg cattagccgg tcttttacat cttcggttga atctttgatt gaatcaccat
     5461 aaacagagat tttctttttt cctgatttga tttttgctaa cattcccatc aaagaatcca
     5521 agtcactatc tgaaagacca tctacgattt ctagtaattt ttcatttcca tcaaatttgt
     5581 catagacgaa tttttttact ttcgtccgag tagtcatatg atggatcttt tccattactt
     5641 ttcccgatac aacgactgct gtcgctacac tggcagcggc tgctacgctt aatccgattg
     5701 ttacttttgt tgatgttttc ataagtatca gctccttttt atctatctcc atcatagcat
     5761 agacaaaaaa ataaacgaac taatccactc agtgaaatct caatccttta gggaaaaagt
     5821 tctttactgt tccttttact tgtttcccaa atccgacagg tatcttatct aatactagca
     5881 aaacccatcc ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt
     5941 ccttttgagt gatcggcaag caaggtatat tctctatctt ttttgtagca agcgctaatg
     6001 catagctagg ttcaaatcga ttctttttga aatctcctaa atgcaaacct gtacgtacga
     6061 cttttaaacc atcgagagaa ggggctaact caggaacttc ccaaagatgg tcgttgaaca
     6121 ctaaaagtct tccagtagct tcataatgaa aatcatttga aaactccgtc cagagttttt
     6181 cctgctcttt tgtcatttgc actttacttt tttttcgtgt cttcttttct ttatgcatct
     6241 gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga tctttatgag
     6301 gccatatacg tattgttttt tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac
     6361 taacggattg agtcaaagga atctcctcaa tcgttactgg ataattttct acaagccagc
     6421 taatgatctc ttcattttct tctggtgcaa acgtacaagt cgaataaatc agttgtcctt
     6481 tattttttag catttttatt gctgaagaca aaatctcttg ctgacgtttt tgacagtata
     6541 gcggagattc ttctgtccac tcttttattg catttggatc ttttcggaac attccttctc
     6601 ctgaacaagg agcatcaacc acgatccgat caaaaaagcc cgaaaaatga gggacaagtt
     6661 ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc ccatcgttct atattttctg
     6721 ataaaatctt cgcacgtttc ggaaagatct cattcgtcac taataaccct ttccccttca
     6781 tttgagcagc taattgggtt gatttaccac cgggagccgc acacaaatcc aaaacttttt
     6841 caccgggttt tgctgcagca gctgttccaa cgatcattgc acttggttct tggctatatt
     6901 catacccagc ttgatggagg aaagactttc cattgacagt gccaagaaat ccctcattgc
     6961 tgtagggagc gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg
     7021 gttttagtgg attccaccga aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa
     7081 aatcggatgc ttcttcgcct aaaagaagac gatatttttt gataaattgc tgtggtaacg
     7141 ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt ttacaatctt ttttacgaaa
     7201 aataaaggtg gctgatctac tttttcatgt tgctgcctaa ggtgactgtt cctttaagat
     7261 attttccaag tattcatccg tcacctcgtt tttatcccaa acagcgttca tcatttcaag
     7321 aaaatcttga gaagaatcat ctgagcgcaa acttcccaat gcattaagcc ataccaattc
     7381 aagcgacttt tcttctttga tcttcgtgtg acctttgaca tatgagaaaa atggccgctt
     7441 aaatagcgaa tccatgtcta aatcagattt tgcaatgatt tcacgaatac tttcatggat
     7501 agaaaaaaaa tctttcaata agtagacaat gcttaaaaca tcatcggaac tgcattcagc
     7561 taagtagttt tcaaaataaa cagtgatttc ttctaatatc ttctctttta acccgctgat
     7621 tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg cagaactctg ctaactctct
     7681 tgcagtaatt gacttcattc catattcttc cacgtatacc atacttttga acagcaaata
     7741 ttctttacta taatttgtta ctctccccat cattcttacc tccgtctctt ttcatctacc
     7801 cataaataaa taacataaat aattctaaaa atattattga taattatcaa aacatattta
     7861 gttatatcca ttaaaaatca atttttccgc cttaaaaaca agaatatctt gacaaaatac
     7921 cctaggaaat aaacaataga ataaacaagg taacaaaaaa attaaataac gttatataaa
     7981 aaagaaggcg acactctgtg agaataacac agactgaaat gactttccac tcacagtttt
     8041 tgtctaacta tatacaaaaa cagaataaaa aggaggtatt tatgctaatt gaagcaagaa
     8101 aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa taattgttct ttacaagaat
     8161 tatccaatga cttacatatt ccaaaacgga caatcaaaga actgatccga aaaataaatg
     8221 tatcactcaa gcaattttta ttgattgatg aatttattta ttctaatcat aaaggagaga
     8281 tccaaaccaa agaaaccgat caaactaaaa agttagctgt tttttctaag ttaaaattat
     8341 gctacctaaa agaatccaat cgttttaact atttgatgtt actaatcaat tatccacaaa
     8401 ctgctgtacc aaaaaaatat ttacttgaac aactttatat atcgccctcc tatctcgaaa
     8461 aactgacaaa acagctcaat cattccttaa aaaaattcca tttacggatc ctatctgtaa
     8521 aaaattgctg tactcttgaa ggaaatgagt tatctatccg tctctatctt tacttttttc
     8581 tttcagatac atttggaggt aacgattggc cattttcctc acttgaatta aatgcactaa
     8641 aaatgaacag tacagatgag caaatagaaa aaatttcaaa acaagaaaaa aatatctact
     8701 tgctgttaac tttattcata ttacgaaaca aacaacaggc atattttcca aaaacttctt
     8761 ctgaaatcac tgaattgatg gacttattgc agcatacaca tgattattca gaatacttta
     8821 cttctttttc agtgagtcgc ccttgtgaag agacagaaac agagagagcc tactttaatt
     8881 attttttcta ttgttttttt ccaaatcaaa tctcttttcg acaaaaacag gagattggtt
     8941 ttttattttc cacaagcaaa aaccttattt gtcgacaagt cggtcaattg atgacttact
     9001 taactcaaca gtttcctctc aaccatttag aaaataaaat accaatttat ttttattttc
     9061 ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc cagcagtagt ttttctacac
     9121 tttgctttcc agaagccacg tccaatcatt ctagttcttt tatacaggaa aagaaattaa
     9181 gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc
     9241 ttagtcaatt gatccaacac ttactacaaa cagaaacgaa agagcagtta ctcatccatt
     9301 tgcagataac caaacagttt acaggaaatt atcatcttca aagccatttg cagcagttat
     9361 ttcgcaaaga tgctgttaaa attaccgcat attcagaaca tgctgatttg atcattacag
     9421 atcagcatga gaaaatttca gatgaaacgg agactttcta tctggataca acgcacaata
     9481 cagtgttatt tggcaagtta ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg
     9541 agaacaaaac aaagaggatg tgacaaaatt cttgtcacat cctcttattc caaataaatg
     9601 gtattcaatc agctaactct cggtattagc tcagatcaaa gcgctgattt cacaacttct
     9661 ttattttaat ctcttcggtt gccgccgaac aataagatca agcgcaacag ctgcaggaat
     9721 gtcgaaagcg ctgctgcaac ataagtcagt gctgctgcaa ataatacgtg tcttgccatc
     9781 ggtacttctt cttccgtcag tagtccgcct tcactcaaaa tttggattgc tcgccgagac
     9841 gcgttaaatt cgactggtaa tgtcaccaat tggaaaagta atgctaaaga aaaagcgaaa
     9901 atcccgatat tgattaacgt agagttccag ctaagcaata cacctaccat aatcaagggg
     9961 aacgaaatca tcgaaccaaa gttagctgct ggaacaagtg ctgcacgaat cttcaatggg
    10021 acataacccg aagcatcttg taccgcatgt ccgcattcat gagctgctac acctatcgca
    10081 gcaactgaag tagactgtgc agttgcttga gacaaactca acatcttatt acctgaattg
    10141 tagttatctg tgagatctcc ggcaatttgt tgaacaccga cattatttat ctgttgactt
    10201 tgtaatatat attgtgctgc ttgtgtacca gtcacattgt ttttgcttct cacttgatcg
    10261 tattttcgga aagtactatt gacgtacgct gacgcaattc cggaaataac taatccaaga
    10321 atgaccaaaa gaaatgtcgg atctaaaaaa ccatacattc cataaccata cattttcgtt
    10381 tcactccttt tcgtttactt tattttatca ttagtagtag gaaaagaatg tgaaaagacc
    10441 ttgaatattc tttaattctt tttcacaaat tccgatttca gtttcatcgg tccaaatcct
    10501 tctaccttgc aatcgatatt atgatcgcct tctactaaac ggatgttctt gacttttgtt
    10561 ccttgtttaa tggcacctga cgcacctttt actttcaagt ctttaatcac tgtcacgcta
    10621 tcgccctcta acagacggtt tccatttgca tctaatacca ctaatccgtc ttctatgaca
    10681 gtttcttgtt cttctttgtt ccattcgttc ccgcattctg ggcaaatcaa caagcctcga
    10741 tcttcatatg tgtactctga gccacattct gggcaatttg gtaatgtcat cttctttctc
    10801 ctttaaaatc tttagtgagc actattgtaa cggaaaaaga agatcttgaa aagatacatt
    10861 tgtgattttt actacaaaag gcacgttttc gaacgaagta tcattctgtt tttctgctct
    10921 tatacgattg gcatatatct tggatctctt tatagatttc ctctacttct gctaaatctt
    10981 ttgcattcgc accacttgct aatggatggc ctcctccatg atggcgttta gccaattcat
    11041 tgatgatcgg ccccttagaa cgtaaacgta cgcggtaata ccctgaaggc tgctgaacaa
    11101 aaatccccca agccagcact tcgtcaatca ctccaggaag tgaaacgatc gaagctgtct
    11161 ctgaatcatc gattccatat ttatcaagaa tttcttgcgg aaggattatt cttcctgcac
    11221 cattttcatc cacctggata ttttgataaa gataaccgga aagtttagcg actttcatcg
    11281 gcatttgttc aatctctctg ttcaaagcac ttgcatcaaa tggataattt ctcaatatcg
    11341 aagcaatctc taatgtacgg gacgtcgtgg agggatacaa aaagcgccct gtatctccga
    11401 cgattcctgc ataaagtaac cgtgcagcct taacgtttat ctttaaagat tcaggaaaca
    11461 tcaaagcaaa ttctgcaatc atctcactac agctacttgc ttttgtattt acccacacaa
    11521 gatctccata agggtcatca ttgggatgat ggtcgatttt gatcagtttt tctgccaact
    11581 gatatctttg atcactgatt ctgggtgaat tagctgtatc agtcacgatg actagcgctc
    11641 cttggtagaa gacatcttca acttcttgca tttctgctaa aaagcgaagc ccttcaatcg
    11701 tttcacctac ttgacgtatt tctttttctg ggaaactttc ccgtaatagt tcggctaatc
    11761 ctacttgtga tcctaatgca tcaggatcag ggcgcaaatg cctgtggata atgattttat
    11821 cgtatttttt tattgtatct actatttctt gctgtactgt catttcactc ctgctttcta
    11881 agaacgttcc attacttgac aaacaacgat tgctttggaa acgatggtgt tttcaataaa
    11941 cacttcgata tccagtttag cggaacgtct gccgatttcc aatacacgtg gacgaatgtc
    12001 taattcgctt tccaattgga tcagccgcaa ataatgtagg ttcacttgtt caatcaagat
    12061 atttcgtttt tggttcatca ccattgtttt ttgtgcgaca tcagaaatga tttcgctaag
    12121 tacgccaaaa gaaatggttc caacgccatt gaccatttgt ggtgctacta cgaatttgaa
    12181 cttagggttt tccaaacgat ttccttcacg gtcagtatca atcgtatgga tatttccaga
    12241 tatctgatcc gaaatcgtat ctgcaatctg cggttgtcgc tggacaagct gcatcgcttt
    12301 cattacatct tgccgggtaa tcaagccttc caatgtcaag tcatcggaaa cgaccggcat
    12361 gacttctagc ccatcccaga tcatttgatg gctaacagag gcaatactca ttgttttctt
    12421 tacactgatt ggttctttcg tcatcacccg atcgatgatc tggtttggcg atttccctaa
    12481 aacatctttt gccgtaacaa tccccatcaa acgatgatgg cggttgacga ctggaaaacg
    12541 cgaatgctgc gtttcttctg acaaacgctg ataatcagaa atcgtatctg tctgctgcaa
    12601 ataatgagtc ttttcagctg gcatatagat atcactgaca agcatgatat cttttttgat
    12661 caattggtcg cttaacgctc ggttgatcat ggttgcgact gtaaatgtat cataagaggt
    12721 gcgtaagatc ggcatttcaa gatcatcagc tagctttgca atctcagcag tcgtattgaa
    12781 tcctcctgtg atcaatactg cggcgccatc ttccaatgct aatttttgga cttccgcccg
    12841 gttgccgacg atcattagtg atcctggcgt aatatagcgt tccatggcat gtttagtcat
    12901 cgcgccgatg acgaatttat tcaagacctt gtctaatcct gccgccccgc ctaaaacgtc
    12961 cccttcaatg atcctgacga cctcgccaaa tgtcaaacgt tcaatatgtt tttttaattt
    13021 gcgttcgata cggatggtcc ctactcgttg aattgttgaa actagtccga cattttctgc
    13081 atctttgatt gcacggtacg ctgttccttc actgacattc aattcttttg cgatgcttct
    13141 tacagaaatt cgattgccaa caggtaaact ttcaatatat tctaaaatca aatcatgttt
    13201 tgtcgccatt ttctgcctac acttccagcg tttccgagac gctcattatt tttccttcac
    13261 tatcttccag taactctaca aaagattcgg gatcttgttc aatgacaggg aaagtattgt
    13321 aatggattgg aactactttc ttggcacgaa ttgtttgcgc agcctttgct gcatcttctg
    13381 gccccatcgt atagttatcg ccaatcggca agaaagcgac atcaatatcg aattcttctg
    13441 caagcaatcc tagatcactg aaataagcag tatcccctgc atgataaatc gtttttcctt
    13501 ctgcttgaat aataattccg ctaggctcgc ccatatactg aacgataccg tctacttcat
    13561 agcttgaact atgctgtgca tgtacgaatt ttaccacgcc gaatggaaac gcatgttttc
    13621 caccgatatt cataccgaat gaatctactc ctctgctgtt cgcataatca gcaatctcaa
    13681 caatactgat aattgtggct tgattttctt ttgcaatagg aatcatatct cctatgtggt
    13741 cactatgacc atgagtaatc agcaaccaat ctgtctttac ttcttctgca gttaagtctg
    13801 tcaatggatt tcctgtaata aacgggtcaa tcatcacgcg ttggccattt tccatttcaa
    13861 ttgttaaaca ggaatgtcca tgatacgtaa ttttcatatt ttacacctcg cgttttctct
    13921 gtatcttttt tattaaaaac gatttcatca cttgcacatc aatgaaaaaa tatagctgct
    13981 aaatatattt tcattggatt gatagtacag tatgtacaaa gcaattgtaa cagataatac
    14041 aggatgcacc aagtaccaag cagatataat ctcaagtttg aggtctctgc tctgcttagc
    14101 ttctcttctc gtcccttaac taattaaatc agcgtaaata ggatggacga tcttttctaa
    14161 atcggctact tttatccgaa ggcttctccc gatttcaccg cttgacacaa ttacttcttc
    14221 tttttctttt aaagaactgt ctgctaaaaa acaataatca ggatgtgcga gttttattcc
    14281 aataggggtg tttgctccgt ggggatatcc tgtatgcatt aatacaaaat ccattccagg
    14341 aaaaccaact ttacgatctt tcgtcacttg tctcgttttt ttgtaatcta agtgatcgtt
    14401 caaaggaatc aatgcgatga tcaccccagt tttgtttcct ttcactacga gagttttaca
    14461 tatcaaagct ggatcaatcc ctcttttttg tagttcttct gtgaaagatt cttctaccac
    14521 atgactaaaa tcaagtggtt gatagctgat cttttgttct tctaaataac gttcgacttt
    14581 tgtttgactc atcattgatt cactcccttt attctatcgt ttattataaa gcgagggatg
    14641 ctgtattcaa aaattatttg tttttcgcaa aaaaagagca agacgagcac tctatagaga
    14701 ggttcatctt actctttttg tttatttttt atttcgacgt tgagtagctt ccataatgac
    14761 tggcaagatc actgggcggc gttttgtttg ttcaaataag taacggccta attgatctcg
    14821 gatgtcttgc tttaatttgc tccactcaaa atcattgctt tctaaatgtt tctcaacgat
    14881 tgtttcaatc atattgctgc tttctttcat cagatcacgg ctagctttta cgtaaacgaa
    14941 tccccgtgaa gtaatttgag gttttgaaat gattttcttt tctcgccgac tgatagtgac
    15001 aaccgctacg aaaatcccgt cttctgacaa gatttttcga tcacgtaaaa caatgtttcc
    15061 gatatcacct atgcctaatc catcaatcat tacattttct gcacttgtag ttcctgcaac
    15121 actcatacgc ttgttcttat attccagcac gtctccgcgt ccagtaatat agatattttt
    15181 gtatggcatc cctactgcat gagccaaatc tgcatgagca gctaattcac gatactctcc
    15241 ttgaactggc acaaaatatg ttggtttcat caaattgatc attaattgca agtcattagg
    15301 attggcatgg ccagaaacgc gcatattctc agagatcaac ttcactgttc caccagctcg
    15361 ataaatgata tcttccgttt tcgccacgat ggtttccatc gcgatgcttg gcgtagtagt
    15421 gatataaacc aaatctcctt cttcaatctt gacattacgg tgtgtaccgt ttgccatttt
    15481 ttgcagagac ttgatgggtt cacccattcg gccagtttct aaaatgatca gttcttctgg
    15541 atcatagttt ttcatgtctt tcaacgtaac aagcagatct tcacttggta attgaagttt
    15601 ttctaaacgc atcgctgtgc gaataattcg ttcgaaatct tgaccagtca agacaacttt
    15661 tcgttgtgct ttttctgctg catccaatac ttgttgcact cgttgcaaat tacttgctac
    15721 acaagcgacg atgatacgcc cttcccaata tttgatcgta tcaaatactt catctgctat
    15781 ttgggcttca gaagctacag gctgaggatt ttctgcattt gccgattcac ttagtaaggc
    15841 taagactcct tctttgccga tttccgccaa tcgtgcataa tctgtttgat acattgggat
    15901 agccgtttgg tcaaatttaa aatctcccgt ataaacgata ctaccttctt ttgttttcag
    15961 attgatcccc actgaatctg gtatcgtatg agtggtacgg aaaaagctca ctactgtttc
    16021 accaaagtct atttctgtat gctcatctac cacatggaaa tccttaaatc ctttggctgc
    16081 ttcattacga ctgacattca attttgctaa ctcgatcgtc aactctgtcc cgaatactgg
    16141 tacagaaatt tttgacaaca agtaaggaag tgccccgatc gcatctgcat gtccatgtgt
    16201 taaaaagaca ccagctacac gatcaatgtt ttcttctaaa tacgtaaaat cagggattac
    16261 gacgtcgatc cccaacaact cattttcagg atattttagc ccacaatcta aaataaaaat
    16321 atcctcgccc acttctgcga catacatgtt ttttccattt tcacgaacgc cgcctaatgg
    16381 gataatttta atggtactca caaacttcac ctcttcttat gacatccaag tttaaagatg
    16441 tcgattttct tctttatcta aatatatgag ccattccaat gaagcatatg caaaatcact
    16501 atttctcaac ttatggagaa tcagcaggaa aattaccagc acatccgcgt tctgacagtt
    16561 ggatgggcat tttattttgg atatacttca ttaacaaaat ggatcttttg cgaaacgcta
    16621 tagccagaat acttatataa gtattcagct gttccccatt attatacaac aaataaacta
    16681 aatcgctagt atcagcaatt gttagaacaa ctatgcacgt aaaaatccct ctgtagcatc
    16741 taggatttct tttgctattc ctctttcatt tcttcttcgt cggtttgaaa catacttttt
    16801 tcattttacc acattttttg caataactat agtgactttt tatatataca gaacctcatg
    16861 ttgatggtgt gcaaacagca taagatttaa gctagcttta agataggttg tctattatca
    16921 aatgcatcta ctaggaggga tgcaaaatat gaattttatg aaacgtgcat ggaaaagtac
    16981 aaaagtaaaa tggggacgtt cattgttatt gtttgccgtg ttcacagcca ttcttatttt
    17041 tgtgctagct ggcctcacga ttcgaagcgc tgcagaagaa gcagcgaatc aagcacaaaa
    17101 agaagttgga gcaacagtga ctttaagtgc caatcgagaa gcttcattta aaaaacagga
    17161 acaaacttct tcaagcggtt ctaccactga agaacgtcca gatccaggca gtttttctct
    17221 tacaccagtt tccttaacgg acgcggaaaa aatagctgaa ttagataatg taaagagtta
    17281 ttcctttgaa agttccgcct cagctatggc aaaagatgga ataactgcca tcactagttc
    17341 tgattcaagt gaagacagtt cagaaactac tgatgcaggc ggtgaacctg ctggaggaat
    17401 gggcgggaat ccgcaaatga ctcaagcaga ctttcaaatc agtggtgtgt cagcaactgc
    17461 acaaaatagt aatttttctg atggaacagc aaaaatcacg gatggtgaag gaatagatga
    17521 cagcgacaaa ggcacaaaca atgtagtcat tgattctact ctagcagaaa caaatgacct
    17581 ggcagtcgga gatactttca caatcacgag tactgaagat gaagatacca cttatgaaat
    17641 gacgatcaaa ggaatttatg aatccagcga aacaagcggc agtatgggta tgaaattcaa
    17701 cttcatgaat ccgtcgaata cactttacac ttcttacact tttgcaaatg aactaaatgg
    17761 gacatctgac gataacacga tagattctgc tatttacact ctatctgatc cagataaaat
    17821 ggatgagttt ctcaaagaag ctgagaagct gatcgacaca gatacattta gcttacaatc
    17881 aaatgattcc atgtatcagt caatgttgga acctttaaac aacgtggcaa gtttttctaa
    17941 aaatgttgtt ttactagtgg ctgtagccgg tattatcata cttactctga tcattatgat
    18001 taccattcgg gagcgtcggc atgagttagg cgtcttgcta tctttaggag aatccagaag
    18061 taaaatcatt ctccaattgt ttaccgaagt ggctatttgt atgattcttg cattaggagt
    18121 tgcttcgctt agcggaaacg tcgtcgcgaa tgccgtggga cagcagctac ttgaccaaca
    18181 gacagaaacc acgacacaaa accagcaagc tcctggcggt ggacaaatgc caggtaacag
    18241 acaagaacaa ggcggtcaac gcgatggtca acaaggaacc acgaataatc catttgctgt
    18301 ttctgatcaa gtcagcgaat tggaaatcaa tgtcaaacct gcacaattgg gactattagc
    18361 cggagtagca tttggaatca gtctgttttc tgtcttttta gcttctatag ggatattaag
    18421 attaaaccca agaaaaatcc tattaaacta aaggaggaat ttgatgttac ggacagaaca
    18481 attgggattt tggtatcaag atccgcaaga tgcgctttat aaagatgtag aactgaattt
    18541 cgaagctgga aaaatgtacg ctattttagg agcaagtggt tctggaaaaa cgacttttct
    18601 ttcgcttatt tctggactag atgcaccaaa agaaggaaag gtatactatc aagatcgttc
    18661 actgaacaaa atcgggttaa gaagttatcg taaaaatgat gtctcgatta ttttccaagc
    18721 ttacaacttg cttccttatt tatcggctct agacaacgtc atagcagcaa tggaaatcac
    18781 taagaccaag caagagaatt atcgacaagt tgctttagat cgactggctc gtgtaggaat
    18841 tgatgaaata ttagcaaaaa aacaggtgac caaactttcg ggaggtcagc agcagcgagt
    18901 cgctatcgtt cgtgctatct gctgtgaaca tcaactaatc gtagcggatg agcctactgg
    18961 aaatctagat gaaacaactt cacaagatat cgtcaaactt ttccaagaaa tcgcccatga
    19021 acaaaatcgc tgtattatca tcgtgactca tgagattgaa gtagcaaaaa gctgtgatga
    19081 agtctttgaa ctaaaaaata aaaaatttca aaaaatcgaa gtttaaaaaa caggataatg
    19141 atcaaataaa aaatccgaac catatctgtc tcgcagatat ggttcggatt ttttatttgc
    19201 ttgattcaat tagtaataag tggtttttat tttttttcgt cttttcgaga aaacttgtct
    19261 ttgacttcgt cgcctaaatt tcgagctttt tctttgatgt cagcacctag tgattgtgct
    19321 ttcccttctg tttctttgct cttgtcgtct gttgctttgc cgtatgcttc ttttccttca
    19381 ccgactactt tatctttcat atctttagaa cgtcctttta aatctgccat tgtgcttcct
    19441 cctcttagtt tgtttattga tctaccttca gggtaacatc aacaacagat acagacaaac
    19501 agaacgctcg ttttctaaaa aagatagtta gagctgttga ttaaaaactt ttttcgaaag
    19561 tcccccatac tttgacttct ttttcttgga ccattatttc tcctttagcg ataactgtat
    19621 caatatcaag cgtttcttta tccaagatac acagatctgc atcagcacca atttcgattt
    19681 ttcctttttt atccaatttc aatatacgtg ctggattact ggttatagct cgtaaggcga
    19741 tttccaaagg gatactttct ttttgcacag cttctttgat ccctactagc aatgccttgg
    19801 cacttcctac tcctaaccct aggaaatgat tgttttcatc aaaataaggc aagcttcctt
    19861 gtccatctga ggtcatcgtg aaattatcta aagaaacatc ttcctcgatc aatcgtttca
    19921 gtgctttgct gaagcgaact tcaccatctg tcttctccca aaaatcagga tcttcgctag
    19981 cagtgaagtc aatcgtgcca ccacgtttgg caaatgccac gcaagcttca aataattctg
    20041 gtgtgcgatt tgcatgagta ggcaaaaatt gtgtgatcgg aatttctgtt tcttctacga
    20101 ttcgtgtcaa tagctctagc tttctttttc ctccgcctaa gtggacattg atgatcccag
    20161 cttttccgga taacatgcct cctacacgag catctgctac tgcacgagcg aactcttcaa
    20221 aacttggctg agaagaacgg tgatcggata ctgcgatttc tccgattccg ataattttat
    20281 caattgtcaa aaagtctttg atgatagaat ctgttactgt ttttaccggc aatcggtacg
    20341 aaccttcgta tacataagtg gagattcctt ctgcttccag acctttcgct ttgctgatca
    20401 acgcagtcat atctctacct tctccgtctg ttcccaaaca accgacaact gtcgttacac
    20461 cagctgtagt caattggctc aatgtgactt ctggcgttcg attttgataa ccgccttctc
    20521 ctcctccacc taaaatatgg aaatgacagt caatgaaacc cggggttagt acttttccct
    20581 cacctgaaag ttcttctact tccactcctt cgtagccgcc ttctagctgt tcttctacag
    20641 ccaaaatctt cccaccggca atcagtacat ccatttttcc taagtaatct ggtgcaaaca
    20701 cttcaatttg tcgaatgatt ttcatacatt tttcctcttt ctttaagtat aactgatgat
    20761 aggatcttaa aaaataatcc aaacgatagg atctttactc ctttgatttt accgtaaaat
    20821 gacggttctt ggagtcctta tctactgttt ggattacttt tttatttaca ggtctccagt
    20881 actactgcag acctattgca atggcaatca tgatgaaaat agttcctaaa acaaacagga
    20941 atccttgcat cttgatttgg aatcttgccc attttgccca ttcgatccgt gcaacaccta
    21001 gcacaccaat caagctggct gatacgggtg taaaagcgtc cataaaacct gaaccaagct
    21061 gataagcaag aactgctact tgtctggata cacctactaa atctgccagt ggagccatga
    21121 ttggcatagt caaagctgct tgtcctgaat ttgatgtaac aaccaagttg aacaaacttt
    21181 ggaagatgta catcgcccaa gcaccaatga cagctggaac accggtcaac gccgtcccga
    21241 tcccatgcaa aatcgtattc aaagctgaag gaacagatgc atcagatcct cccaagacaa
    21301 gaagaatccc tttggccata cctactacga ttgctgtacc agctaaatca gctgcaccag
    21361 attggaaaga agaagcgatg tcattgactt tcattccatc taatttgaaa agaacagcaa
    21421 tgattccagc aactaatccc atcacaaaga attgagaagc gatttctgga atgtaatagc
    21481 ctttttgagt cacgccccaa acgatccaga caaggacaac taacatctct aataagatca
    21541 atttatgccc aagcatgaat tttttatttt catcagatgt ctttttgata tgagaacgga
    21601 aataatcatc tgatttgtaa gtcaacgaac tttctggttt tttacggatt ttttctgcat
    21661 atatcatcat ataaccagcg gctaatgcag taacaactac ccacataatc aatcggaatg
    21721 tcgcaccaga caacacgggg attccagcaa tcccttgagc caccgctacg ctaaacgggc
    21781 tcatccaaga ggtcgcattt ccgacttgag atgcgacata agtcacggtt actgctacga
    21841 tcgagtcata tcctaacgca atgacaaatg gaaccatcac cattgagaat ggaattacct
    21901 cttctgccat cccgaatgta gcgccaccga atgaaaaagc aaagaataat agcggtaaag
    21961 ctaaacgctc cagtccctta gttttactga taaacgcata gatacctgcg tcgatcgctc
    22021 cggtacgcat aataatacca aaagcacctc caacaacaag gatcaatgca gcgatcccta
    22081 cagcagatcc atacttatct ccagatacta gtccttcaaa aacaaaattt aagaaaccaa
    22141 agccgccaaa atcatctgtt ccccatattt tggctgtttt atgcaatttt ttagaagtat
    22201 catagatatt ttcgccgtac tgatcgtaaa gcacatcatc tgtcaaggag atttcatcta
    22261 gtttttcctg cgtaagattt ttttctccgt cagcaatgac tttttccaat cctttttccg
    22321 gaacatccag tttttcacgt tctgctggat gatcttgcag ttcttctaat tgatcaaaca
    22381 catacgactt atccagctcg taagcataac ggaaggaatc ttgacgtaaa acagtacgag
    22441 tacttgtttc gccatttgca tctttgtatt caatatcttg cgtgctgaac tttcctgctg
    22501 gaacgacaaa agtcagtatc catgcaaaga tgactacacc aaaaataatg acatacgtat
    22561 ggggtgtcga taatttagtg aagtcgcgtt tcggttttcc tgaagaattt gttttcttca
    22621 aatttatttc ttcctttctt ttttcagtaa ttttgatgaa taaaaaatgg ataatcaaat
    22681 gaattataca gtaaaaatga catttccaca tgaataattt attagaaaat cacttttttc
    22741 tattttgttt ttttctccta tgtatgatta tttttttctc tttccattta tattaagtat
    22801 tttatttctc tgtttttcct taa
//
LOCUS       DACDVT010000043        21746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDVT010000043 DACDVT010000000
VERSION     DACDVT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.043"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            348..604
                     /locus_tag="IWL52_001657"
                     /pseudo
     CDS             348..604
                     /locus_tag="IWL52_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            991..1218
                     /locus_tag="IWL52_001658"
     CDS             991..1218
                     /locus_tag="IWL52_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9778208.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1234..2400
                     /locus_tag="IWL52_001659"
     CDS             1234..2400
                     /locus_tag="IWL52_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9778209.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2635..3678
                     /gene="hrcA"
                     /locus_tag="IWL52_001660"
     CDS             2635..3678
                     /gene="hrcA"
                     /locus_tag="IWL52_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9778210.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3772..4335
                     /gene="grpE"
                     /locus_tag="IWL52_001661"
     CDS             3772..4335
                     /gene="grpE"
                     /locus_tag="IWL52_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9778211.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4386..6215
                     /gene="dnaK"
                     /locus_tag="IWL52_001662"
     CDS             4386..6215
                     /gene="dnaK"
                     /locus_tag="IWL52_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9778212.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6366..7532
                     /gene="dnaJ"
                     /locus_tag="IWL52_001663"
     CDS             6366..7532
                     /gene="dnaJ"
                     /locus_tag="IWL52_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9778213.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7900..8637)
                     /locus_tag="IWL52_001664"
     CDS             complement(7900..8637)
                     /locus_tag="IWL52_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9778214.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8639..10141)
                     /locus_tag="IWL52_001665"
     CDS             complement(8639..10141)
                     /locus_tag="IWL52_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9778215.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10400..12394
                     /gene="uvrB"
                     /locus_tag="IWL52_001666"
     CDS             10400..12394
                     /gene="uvrB"
                     /locus_tag="IWL52_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9778216.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12404..15220
                     /gene="uvrA"
                     /locus_tag="IWL52_001667"
     CDS             12404..15220
                     /gene="uvrA"
                     /locus_tag="IWL52_001667"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9778217.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            15483..16748
                     /locus_tag="IWL52_001668"
     CDS             15483..16748
                     /locus_tag="IWL52_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9778218.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            16752..17483
                     /locus_tag="IWL52_001669"
     CDS             16752..17483
                     /locus_tag="IWL52_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9778219.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            17618..18502
                     /gene="rapZ"
                     /locus_tag="IWL52_001670"
     CDS             17618..18502
                     /gene="rapZ"
                     /locus_tag="IWL52_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9778220.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            18499..19497
                     /locus_tag="IWL52_001671"
     CDS             18499..19497
                     /locus_tag="IWL52_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9778221.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            19531..20466
                     /gene="whiA"
                     /locus_tag="IWL52_001672"
     CDS             19531..20466
                     /gene="whiA"
                     /locus_tag="IWL52_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9778222.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            21034..21690
                     /locus_tag="IWL52_001673"
     CDS             21034..21690
                     /locus_tag="IWL52_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778223.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
BASE COUNT         7184 a         3713 c         4727 g         6122 t
ORIGIN      
        1 cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta tataagacta
       61 agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat tttttaaata
      121 acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg aggattcttt
      181 ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat ttctgaactt
      241 ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc ctacattttc
      301 acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg agattcttta
      361 agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc
      421 tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa aactattcga
      481 tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat aaacattgga
      541 taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg gtgggctgga
      601 atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt ggcaaatacg
      661 tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag tagggcaccg
      721 aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa ttggaatcta
      781 gttaaaactc ttgaagtgag agatttgact agattttttt atgaataaaa taaggacaaa
      841 attgtttagg aagaatcatg agtgtaaaaa aatcatccaa gaagtcgatt cttggatttt
      901 ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg taagcaaagc
      961 gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat ttttgcagat
     1021 tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc tgtaaaagca
     1081 ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct tgattctaaa
     1141 tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg
     1201 cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa tcccgcagaa
     1261 aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta ttactgtgac
     1321 tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat gctgctgaat
     1381 gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact ttatgtagga
     1441 ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc aggtgctaga
     1501 gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc tggtgatttg
     1561 acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc catgggagtg
     1621 cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc agaagatgtg
     1681 tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat cgatttgatt
     1741 tatgcattac cgggacaaac actagaagga tatcgggata ctttggaaaa agctttggct
     1801 ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac aatgttcatg
     1861 aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac cagaatgttt
     1921 gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt
     1981 gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga ccactatttt
     2041 gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa caaaggacca
     2101 atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga agaagaaaaa
     2161 ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa aaaaacaggg
     2221 attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat
     2281 gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg tgtttatttg
     2341 acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt tgaaaaatag
     2401 tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa agtttgacaa
     2461 tgaggtattg aagtattgat ataactggat ttacatcaat tatttagaat tagcactctt
     2521 gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct tcttgctata
     2581 tttataagtg aattagcact tagaatatat gagtgctaat gagaggtgag gaagatgtta
     2641 acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag tagtggcgtg
     2701 cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg ccagctcagc aacgattcgc
     2761 aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc ttcttctggg
     2821 cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa acctgctcgc
     2881 gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt tcatgaaatc
     2941 aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta tacgactttt
     3001 tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt acccttgaat
     3061 aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc
     3121 actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac
     3181 cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga gattccgatg
     3241 attcttcata aatatttcca gacgacagaa gggatgtctc agttatttga caccgttctt
     3301 ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa tgaatctctt ggattatgag
     3361 ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga ctcagacgga
     3421 cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg tgctgaatta
     3481 ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat agatggtcat
     3541 ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg gatgttcggt
     3601 ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta ccgctcgctt
     3661 gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt tctctctttc
     3721 ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat cgtgaaagaa
     3781 aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga aatcgatgtc
     3841 gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga aacagcgaaa
     3901 ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc tgaaatcgca
     3961 aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg ttcacaagat
     4021 ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc cacagaagtg
     4081 tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt
     4141 aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc ctttgatcct
     4201 aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc cgatacgatt
     4261 gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc aactatggtc
     4321 attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag aaggagacgt
     4381 taattatgag taaaattatc ggtattgact taggtacgac taactctgca gtagcagtat
     4441 tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca acaccttctg
     4501 ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt caagctgtga
     4561 caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag
     4621 atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt caatatatca
     4681 aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag
     4741 cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc gctggtctag
     4801 aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt ttagataaaa
     4861 cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt gacgtatcta
     4921 tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac aaccatctag
     4981 gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc aaaaaagaaa
     5041 acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa
     5101 aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca tttatcacag
     5161 caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa tttgatgaat
     5221 taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgtta aaagatgcag
     5281 gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact cgtatccctg
     5341 cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca gttaaccctg
     5401 atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt gatgtgaaag
     5461 atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg ggtggagtgt
     5521 tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa gtattctcaa
     5581 ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg
     5641 ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca gctcctcgtg
     5701 gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta aatgtatctg
     5761 cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct tcaggtctaa
     5821 cagacgaaga aatcgaacgt atggtgaaag atgcagaagc aaacgctgaa gcagataaag
     5881 ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc attgctattc tctgtggaca
     5941 aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag
     6001 ctcgtgacga attaaaagcc gctgttgaag caaacaacat cgaggaaatg aaaactaaac
     6061 gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa caagctgcac
     6121 aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg
     6181 ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt tcgagagaag
     6241 gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt
     6301 tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca ataggaggaa
     6361 agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa ggagcatcag
     6421 aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct gacatcaata
     6481 aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag attttaagtg
     6541 acccgcaaaa acgcgctgca tacgaccaat acgggcatgc aggcacagat ccaaattacg
     6601 gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt ggcggcttcg
     6661 gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca gtcgatccaa
     6721 atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt gaagaagcga
     6781 tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat acttgtggtg
     6841 gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat ggtgcaggta
     6901 cgatcaatgt agaacgccaa acgccacttg gacgagtgat gagccgtcaa acttgtgatg
     6961 tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac
     7021 atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa gaaggacaac
     7081 aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt ggtgatttgt
     7141 atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg tgaaggttct gaaatttact
     7201 atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa gtaccaactg
     7261 tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg aacttccgtt
     7321 tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag
     7381 taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta cgtgcattcg
     7441 ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc gataagttta
     7501 aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaatat aaagtagact
     7561 tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
     7621 acaatcatac gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca
     7681 gctttagaat aagataatca tcatagtttc tgaaggtggt atcgactatg tacttgatat
     7741 cgtcaatgaa ccgatggtac tttcttatgc gatggaacag gtagagcgat tgcttgtttg
     7801 tgcaggagaa caagcgatgc ggctggtaca gttaagtgaa aaaacaagaa actaaaaatg
     7861 cccggtcgtc atgaagcgac cgggcatttt tggtttttat taaatattta atactttatt
     7921 caagaaatct tttgtccgtt cattttgcgg attttcaaag atttgttgag gcgttccgtc
     7981 ttctaaaaag tttccgtcgt caatgaacat gacacgattg gccacttctt tagcgaagcc
     8041 catttcgtgc gtaacgataa ccatcgtcat accttgttct gctaactttt tcatgacgtt
     8101 caagacatca ccgaccattt cggggtcgag tgcagatgta ggctcatcaa aaagcatgat
     8161 gtcagggttc atcgctagag cacgagcaat agccacacgc tgcttttgtc caccggaaag
     8221 catttcagga tacatatctt ttttatcttc tagaccaact gtctgcagca atttgattgc
     8281 ttttttctct gcttcttctc tagattcttt cttcaggtcc atcggtgcta aaatgatgtt
     8341 ttccaaaata gaaagatgag ggaataggtt aaagtgctgg aataccattc cgatatgttg
     8401 tctcaccaag ttgatatctg tgcttttatc catcaagtgg gcgccgtcaa taataatttc
     8461 accgtcagaa ggttcttcta aacgattcaa gcaacgcaaa aatgtacttt ttcctgaacc
     8521 tgaagggcca atgatacaga cgacatctcc ttcgttgatg gacacattga tgtcgtttaa
     8581 tactgtgtta tcgccgtatt ttttgactaa atgctcaact aagatttttt ctgccatctt
     8641 acttcacctt cttttctaaa acttttgcta attttgtcaa tgctgtgatc aaaatcaagt
     8701 acatgatcgc aatgatcaag taaacatacg tactttgtgt tgtacgtgca acaatgatct
     8761 taccagtttg cagcaattcg actacaccga ttgcggaaat gatcgttgtg tcttttaatg
     8821 agataacgaa ctgattgacg aatgatggga tcatgatgcg tatcgcttgc ggcaaaatga
     8881 ttttttgcat tgtccgatta taagaaagac ctaaacttct ggaagcttcc atttgtccga
     8941 caggaacggc gttgatcccg cctcgaacaa tctctgcgat ataagcactt gcgttaagag
     9001 tcaaggtgat aacaccagca aggaagtcag gaatcgtaaa tccaacgata ccaggcaacc
     9061 caaagaaaat aaagaacgca agaaccatca ttggaattcc tcggatgata tcgacataaa
     9121 tactagcaat tgtgcgaaga gcgcgtattg gtgctacact gaataatcca aaaatcacac
     9181 cgattactat agccaaagca aaggagatca aagttaggag aattgttctc caaagtcctt
     9241 tcaacaatgc ttgatagttg ttttggataa gtccaagaaa tgttgattca tcagcagtgg
     9301 aagactcact ggaagtttct gaaccatccg ctacatattc tttaataatc ttatcgtatt
     9361 cgcctgttct ttttaattct ttcaatcctt cattgaacat attaagaagc tcaggatttt
     9421 gtcctttttt aacggcaaat gcatattttc cgccagattc ttttttgatc ggtgtttcca
     9481 attcttgtcc ttgtttgatc gcataaccga ttaccggata atcatccatc attgcatcga
     9541 ttgcgccaac ttttaacccg tcgtacaatt gatcagcagc atcaaaatat ttgatagaat
     9601 agccatattc agattcatgg tcgcttaaaa attcagcact ttcggtacca acttttgctc
     9661 cgactgtttt tcctttcaaa tcttcataag acttgatcga agtatctcct tttttgacag
     9721 ctaattggat cccgctatca aagtaagaat cagaaaaatc atagcttttc tttcgttcat
     9781 ctgttacagt cattccggca atcattccgt ctgcttgtcc tgattccaaa gcttgcaaag
     9841 cagcagagaa accacggtag tccattcgga tgttaaatcc ttgcagtttg gcgatgtttt
     9901 ccatcaattt tacatcgatg ccttcgtact cgccctttgc gttttgaaat tcaaatggag
     9961 caaaagcaga gtcactggca attacgtata cgtctttttt gggctctatt ttttgcattg
    10021 tctcgcttgt gcttgactct gccgttgtag tgttgtcagc ttgtgcaaca tgtccaaacg
    10081 caagtgtcac aagtcctgtc attaagaaaa caaatgagtg aaataaatgc tttcttttca
    10141 taatcaacct cctaaatttc caatatttta ttattatagg cgaaaagtgc tgaatcagac
    10201 aagactgtaa gctcgaaaaa atgaaaaaaa tgcgagtttc taacatttta tgttataaaa
    10261 cgtaacatgc ataataagat aaaaaagtgg cgaaagtatg ttcggtttgg tataattttt
    10321 tagtaaggaa tgcctttgct ttccttttct ttttattggt cgtaaaatga aaaggaatga
    10381 atgaaaagga ggacgaagaa tgattgagag agatacctcc cgacactttg agcttgtctc
    10441 gaaataccaa ccggctggtg atcagcctga agcgattaac caattagtgg atggcgtagt
    10501 cggcgggaaa aaagcacaaa tcttattagg tgcgactgga acagggaaaa catatacgat
    10561 ttccaatctt attgagaaag tgaataaacc aacactaatt attgcccata acaaaacatt
    10621 ggctggtcag ctgtacgggg aattcaagga atttttccct aacaatgctg ttgaatattt
    10681 tgtttcttat tatgattatt atcagcctga agcttatgtg ccttcaagcg atacgtatat
    10741 cgaaaaagat tcaagtgtaa atgatgagat cgacaaatta cgacattcgg ctactagttc
    10801 attattagaa agaaatgatg tgatcgtcat tgcatccgtt tcatgtattt ttggattagg
    10861 ttcgcctttt gagtaccaaa aacaagtagt ttcgattcga cagggagcag aactagatcg
    10921 caatcagttg attcgtgact tagtcagtat ccaattcgaa agaaatgata tagattttca
    10981 gcgcggtcgt tttcgagtgc gcggtgatgt cgtggagatt tttccggctt caagagatga
    11041 acgtgcctta cgtgtggagt tttttggaga tgaggtcgaa cgtatccgag aagtcaatgc
    11101 actgacaggt gaagttttag gagaaacgga acacgtggca atcttcccgg cgactcactt
    11161 tgtgacgaat gatgaacata tggagcatgc cgtagcgaat atcaaagcgg aattagaaca
    11221 gcgtttaact gtcttgcgaa atgaaaacaa acttctggaa gctcaacgtt tagaacagcg
    11281 aaccaattac gatattgaaa tgatgttaga aatgggctac acgtcgggaa ttgaaaatta
    11341 ttctcggcat atggacggac gtaaagaagg tgaaccgcct tatacgctat tggatttctt
    11401 tcctgaagat tttttgatcg tggcggacga atcgcacgta accatgcctc aagtcagagg
    11461 aatgtataat ggtgaccgag cgagaaaaca gatgctagta gattacggtt tccgtttgcc
    11521 atctgcttta gacaatcggc cacttcggtt agaagagttt gaaaaacatg tcaatcagat
    11581 catttatgtc tctgcgacgc caggaccata tgaacatgaa caaacagata ccgttatcca
    11641 acaaatcatc cgtccgacag ggctgcttga cccagtgatc gaagttcgtc caatcatggg
    11701 acaaatcgat gatctagtag gcgaaataaa tgaacgagtc gaaaaagacc agcgggtgtt
    11761 tgtaacgaca ttgacgaaaa aaatggcaga agacctcaca gattatttca aagaattagg
    11821 gatcaaagtc aaatatttgc atagtgatat caaaacgctg gaacgaacag aaatcattcg
    11881 agatcttcga ttaggtgaat ttgatgtgtt agttggaatc aatttgcttc gtgaaggatt
    11941 agacgtacct gaagtttccc ttgttgctat cttggatgcg gataaagaag gctttttacg
    12001 tagtgaacgt tccttagtcc aaacgattgg acgagctgct cggaatgaag aaggtaaagt
    12061 aatcatgtac gcagacaaag taacagattc tatgcgtcta gcgatggatg aaacatcaag
    12121 acgccgtacg attcagcaga agtacaatga agaacatggg atcgttccaa aaacgatcat
    12181 taaagaaatc cgagatttga tttctattac gaaagaatcg gaagatgata caaaagaagc
    12241 ggtacaagtc tcgtatgaag aaatgacgaa agaagaaaaa gatacgttat tgatgaaatt
    12301 agaaaaagaa atgaaaaatg cggcgaaagc attggatttt gaaacagccg ctaatgtgcg
    12361 agatatgatt ttagaattaa aagcttcaaa gtaggaggac aacatggcta acgataaaat
    12421 tacgattcac ggtgcacgtg cccataactt gaaaaatata gatgtaacga ttccacgaga
    12481 taaattcgtg gtcgttacag gtttgtccgg ttctggaaaa agttccctag catttgatac
    12541 gctgtacgct gaagggcagc ggagatacgt agagagcctt tctgcttatg ctcgtcagtt
    12601 tttaggacaa atggataagc cggacgtaga tagcatcgac ggattgagtc cggcgatttc
    12661 tattgatcag aaaacaacaa gtaaaaatcc tcggtcgaca gtaggtactg tgacggaaat
    12721 caatgactat ttacgcttgt tgtttgcacg tgtaggacat ccgatttgtc ccaacgatca
    12781 tatcgagatt accagtcaat cagtagatca aatggcagat caagtaatgg ctttgcctga
    12841 gcgcacaaag atccaagtgt tggcaccgat cgtggtaaag aaaaaaggac aacataaaaa
    12901 aatattcgaa cgaatccaaa aagagggata cgtccgcgtt cgagtcgatg gtgagatcta
    12961 tgatttaagt gaagtgcctg agctggagaa aaacaaaaaa catgatatcg cgatcgtcat
    13021 cgaccggatc attgtgaaag aagggattcg ttctcgtcta tttgattctt tagaggcggc
    13081 tctgcgattg gcagacggct atgctctggt tgacgtgatc ggtgaagaag aaatgttgtt
    13141 tagcgaacac tatgcctgtc cttattgcgg atttactgtt ggcgaactag aaccaagact
    13201 gttctcattc aatgcgcctt ttggagcttg tccagactgt gatggtttag gtgtcaaatt
    13261 agaagtcgat caagatttag tgattccaga tagaacgaag acacttagtc aaggtgctat
    13321 cgttccatgg aatccaatca gctctcagta ttatcctcaa atgttagcgc aggcttgtga
    13381 aagtttcggt atcgatttag atacaccatt cgaagaatta ccggaagagc atcaggatat
    13441 catcttgaat gggacagatg atcttttcca tttccattat gaaaatgatt ttggtggcgt
    13501 tcgcgatgta gaaacgactt tcgaaggggt attgaagaat atcgagcgtc gttatcatga
    13561 tacgaacagt gatttcacgc gtgagcaaat gcgtctttat atgacagaat taacttgtca
    13621 gacatgtaaa ggttatcgat tgaatcccca agctctatct gttcaaatca atggaacgaa
    13681 tatcgggcaa acaaatgagt tgtctattca aaaagcagta gatttcttct caaacttaac
    13741 gttatctgaa caagagaaag taattgccaa accgatttta aaagaagtaa atgacagact
    13801 ttcattcttg gaaaatgtgg gactagacta tctaactttg agtcgcgctt ccggaacatt
    13861 atctggggga gaagcgcagc gaatcagatt ggctacacaa attggatcga atctttctgg
    13921 agttttgtat attttagatg aaccatcgat cggattgcat caacgcgaca acgatcgact
    13981 gattggctca ttaaagaaaa tgcgtgatct tggcaacacc ctcgttgtag tcgaacatga
    14041 tgaagatacg atgtgggcag cagattatct gattgacgtc ggacctgggg ccggagaaca
    14101 aggcggacaa atcgtggctg caggcactcc tgaagaagta gctaatgatg aaaattcttt
    14161 gacagggaaa tatctttcag ggaaaaaatc aatccctgta ccaaaagaac gccgtaaagg
    14221 taatgggaaa gcaatcaaga tcactggtgc ttcagaaaac aatctgaaaa atattagtgt
    14281 tgaatttcca ttaggagaat ttgtagcagt cacaggtgtg tctggttctg gaaagagtac
    14341 attggtcaat agtatcttaa agaaatcgtt agcgcaaaaa ttaaataaga attctgctaa
    14401 gccaggtaaa ttcaagacaa tttccggcta cgaaagtatc gaaaagatca tcgatatcga
    14461 tcaaagccca atcggccgga cgccgagaag taatccagcg acttatacaa gtgtatttga
    14521 tgatatccgt gggttatttg ctcaaacgaa cgaggcaaaa atgcggggtt ataagaaagg
    14581 gcgttttagt ttcaacgtaa aaggcggtcg ttgtgaagct tgtcgcgggg atggaattat
    14641 taagatcgaa atgcactttt tgcctgatgt ctatgttcct tgtgaagtat gtcatggcaa
    14701 acgatataac tctgaaacat tagaagtgca ttacaaagga aaaagcattg ctgatatttt
    14761 ggaaatgaca gtagaagatg ctgtagaatt cttcaagcac attccaaaga ttcatcgcaa
    14821 actgcaaacg attgttgatg ttggcttagg ttatgtgacg atggggcaac cagcaacgac
    14881 attgtccggt ggtgaggcac aacggatgaa acttgccagt gaattgcaca aaatctctaa
    14941 tggaaagaat ttctatatac tagatgaacc aacgacagga cttcatagcg atgacatcgc
    15001 ccgcttgttg catgtattac aaagattagt agatgctggt aacacagttt tagtgattga
    15061 acacaatcta gatgtaatca aaacagcaga ttatatcatt gatttaggac cagaaggtgg
    15121 agaaggtgga ggaacgatcc ttgcgactgg aacaccagaa gaaatcatta acgtaaaaga
    15181 aagttataca ggtcactatt tgaaaaaaat aatggtataa agaggataga tagctgagat
    15241 agttaaaaaa acataaaaaa atgaattttt gcttgtttta ttaatatcag tgttctagaa
    15301 tcattacatg gtagttcttt ctacactgat tggtatagac aaaaacttcc tataagttca
    15361 atccgttcaa aaaagaattg agcaatcgga ggagtttcct ttgattgctt ttttctttat
    15421 ggtatgatat tccttgtgta gttggaaaag aaaaacaaga gattatgagt aagtgagaga
    15481 taatgatgaa cagtattgaa aatgaagaat ttatccctat attattagga agcgacatga
    15541 acgtttatgg tatggcacgt tcttttaatg aagcatacgg caaaatctgc caagcatatg
    15601 cttcggatca gttagcccct acccgctaca gtaaaattgt caatgtagaa gttattccag
    15661 gatttgataa agaccctgtg tttatcgaga cgatgcttcg tttggcaaaa gaaagatatt
    15721 ctgataagag taaaaaatat cttttgattg cttgtggtga tggatatgca gaattgatct
    15781 cccaacataa acaagagctg tcagaatatt ttatctgtcc atatattgat tattctttat
    15841 ttgaaagact gatcaataaa gtaagctttt atgaagtttg tgaagaatat gatcttccgt
    15901 atcctaaaac attgatcgtc agagaagaaa tgttagtaaa tggacatttg gaacaggaat
    15961 tgccttttga attcccagtt gctttgaaac ctgcaaacag tgtggagtat ctttctgtcc
    16021 aatttgaagg aagaaaaaaa gcttttattt tagagactag agaagagttc gatttaattt
    16081 taggccggat atacgaagcc ggttataaaa gtgaaatgat tgtacaagat tttatcccgg
    16141 gtgatgatag taatatgcgt gtgctaaatg cgtatgtaga tgaagatcat caagttcgga
    16201 tgatgtgtct tggtcatcca ctgttagaag atcctacacc agcatcgatt ggtaattacg
    16261 ttgtgatcat gccagattat aacgaaaaaa tctatcaaac cattaaggca tttttggaaa
    16321 aaatcgaata tacaggcttt gccaatttcg atatgaagta tgatcctaga gatggagaat
    16381 ataaattatt tgaaatcaat ttgagacaag gtcgtagcag tttctttgtg acattgaacg
    16441 gcttgaatct ggctcgtttc gtcacagaag atcgagtgtt taacaaaccc tttgttgaaa
    16501 cgacctatgg aacgaatcaa tcagataaag caagattatg gatgggtgtg cccaaaaaaa
    16561 tctttttaga atatgctcgg gaaaatgaag acaaaaaact agctgagcaa atgatcaaag
    16621 aaaatcgtta cggtactact gtgttttatg aaaaagaccg gtcaatcaag cgctggttgt
    16681 tgatgaaata tatgttccat aactatattc caagattcaa aaagtatttt catgtgaaag
    16741 aaggctagtt catggagaat tttttcagta ttttaggcgg aatgggcacg atggcgacag
    16801 aaagttttgt tcgcttgatc aaccaccgaa cgaaagctac aaaagatcaa gaatatttga
    16861 attatgtctt attcaatcat gcaacagttc ctgatcgtac agcttatatt ttagatcgat
    16921 cagaagaaaa tccaatgccg tttctgctgg atgatattga gaaacaaaat ttattgcagc
    16981 cgaacttcat tgtgttgact tgtaacaccg cacactactt ttttgaagaa ttacaagcgg
    17041 caacggatat tcctatcctt catatgccaa gagaagcagc aaatgaactt gtccgtcagc
    17101 atacgacagg gagagtagcg attctaggaa cagaaggaag tatgaaagct ggaatctatg
    17161 aacgcgaagt caaaaatctt ggattcgaaa cagtgattcc tgatactgtt cttcaagaaa
    17221 aaatcaatta tctgatctat catgagatca aggaatcgga ttatttgaat caagagctat
    17281 attacgaaat cttagaagaa gcggtagagc gcctaaactg tgaaaaagtt atcttaggtt
    17341 gcacagaatt gtcattgatg catgaatttg ctgaagataa ccattatcca gtgatcgatg
    17401 cccaatctat tttagcagat cggacgattg aacgagcgct agctgaaaga agcaaggcat
    17461 tggatacagc atcggaaaag taaaaaaatt ggatttgggg caaatatcct aaaagatatt
    17521 tggtccaaat ctttttgtaa aagaaaaaga aaactggtaa tgatacgccc ggaatgatat
    17581 aattttacta tgcacaatgt gagaggagtt cgtaattatg acagataatt tgcaattagt
    17641 catcataaca ggtatgagcg gtgcagggaa aacagtagct atccaaagtt ttgaagatat
    17701 gggctatttt tgtatcgata atatgccgcc tcgattgatt ccaaaattct gggaattaat
    17761 caaagaatca ggcaaagtca caaaaatggc tttaggagtg gatttacgtt ctcgttcctt
    17821 ctttgaagaa atccaaaaca tgctgatcga aatcgaaaat acgaacttca tcgacacttc
    17881 tatcttattt ttagatgctt cagacgaaga actagtggct cgctacaaag aaacaagacg
    17941 agcacatcca atggcgatgg acggtttgat cactgaaggg atacgtaaag aacgggcgat
    18001 actagacgac ttgaaagccc aagcttccgt gatcattgat acgacgacat tgacaccacg
    18061 acaattacgt gaaaaaatca atgagtcatt caaatcaaag ggagaccaag gatttcggat
    18121 cgaatttgta tcatttggtt ttaaatacgg gttgccgatc gattcggata ttgtgatgga
    18181 tgtgcgtttt ttgcccaacc ctcattatat cccggaacta agaccattga ccggaaaaga
    18241 tcagccagtc tatgattatg tgatgtcctt tgatgaaacg caatcattct accataaatt
    18301 tttggatctt ctggaaactg tattgcctgg atacattaaa gaaggaaaaa gcagcttgac
    18361 agttgcaatc ggctgtacag gcggtcaaca ccgttctgtg gcattgactg aacgtttggc
    18421 aaatgcttta gggaaaaact ataaagtcaa tgtgacacat cgggacaagg ataaaaggaa
    18481 agagacggtg aaccggtcgt gaaaatgaaa acctaccgga taagaaaacc aaagatcgta
    18541 gtggtcggcg gtggcactgg tctgccagtt atcctgaaaa gtttgcgaaa tcaaggagca
    18601 gatatcacag cagtagtgac tgttgcagat gatgggggaa gcagcggtgc aatcagagaa
    18661 tcgatcgcta tggctcctcc tggtgacttg agaaatgtct tagtggcgtt atcggatatg
    18721 ccacaattct atgaagatat tttccaatac cgtttcaaaa aggaagatca gtttttagcc
    18781 aatcatacga tcggtaatct gatcattgcc gcagtttccg agatgcgggg aagcacttat
    18841 gaggcgattc aattattagc aaaaatgatg catgtagatg gacatgttta tccttcttca
    18901 gaaactcctt tgaccttgca tgctgtcttt aaagacggaa cgactgcagt aggagaatct
    18961 aaaatcgcga tcgaccgtaa aaccattgac cgtgtgtatg tctgcaacac aaatgataaa
    19021 agtgaagcca aagcggctcg aaaagtcgtt tcagcaatta tggacgccga catggtcgtt
    19081 ttgggacccg gaagtttatt caccagtatc cttccaaatt tagtgatacc agaaatcggc
    19141 gaagcaatgc tcaagacaac tgctgaaacc gtctatatct gtaatatcat gacccaaaaa
    19201 ggggaaacgg aacatttcac agatgccgat catatacgtg ttttacacaa gcatttgaat
    19261 cagcctttta tcgatacagt attggtcaat acggaaaaag tgcctgaaga ttatatggac
    19321 ccagagatct atgatgagta tctagtacaa gtcgcacacg acttcaacgg attgagaaat
    19381 gaaggatgtc gagtgatttc caccgacttt ttggaattga aagatggtgg tgtcttccac
    19441 gatggagaca aagtagtaga agaattgttc cgtttagttt tcggttcaaa atattaagca
    19501 atttattgtt ggagaaagga ggagaatagg atgtcatttg ctgcagatgt gaagaaagaa
    19561 ctaactggtt tggaagttca tcgagaacat gcgaaagcgg aattagctgc tttgctgcga
    19621 atgaacggct ctttaggtat tgtgaaccag cagtttattt tgaacgtcca aacagaaaat
    19681 gctgcaattg ccagacgtat ctattctctt ttgaaagatc attatcaggt acgaagtgag
    19741 ctactcgttc gtaaaaaaat gaaattaaaa aagaacaatg tctatatcgt ccggttgaag
    19801 caaggcacaa aagatgtgtt gatggatctt gatatcatgg atggtatgat gttcaatgga
    19861 cacgtttcgg acgagattat gggaaattcg caaaaaatgc gttcttactt acgtggtgct
    19921 tttatggcct caggttcagt caataatccg gaaacgagcc gttatcattt ggaaatattt
    19981 tctatttatg aacagcataa ccaagacatt tgcgatatgt taaattatta cggtctacat
    20041 gctcgaacgc ttgaacggcg caatggctat atttcttatt taaaaggagc agaaaaaatt
    20101 gctgatttct taacattgat tggtgcaacc aactcaatgc tccgatttga agatgttcga
    20161 atcgtacggg atatgagaaa ttcggtcaat cgtctagtca attgtgagac agcaaatctg
    20221 aacaagacga ttgatgcggc ttctaaacaa atcgaaaaca tcgaattgat cgaagcaaag
    20281 gtcggtctac atgcccttcc tgaaaaactt caggaaatcg cagaacttcg gcttcaacat
    20341 ccagaggtca gcttgaaaga attaggggaa atgattccct caggtgcgat atcaaaatcg
    20401 ggaattaatc accgaattcg caaaataaat gaatttgcgg aaaaactgcg tgaagaagca
    20461 gtttaatcca aagaaaaaac tgatacatga agcaggagat gcgaatgtat cagttttttt
    20521 taaaagttat acttgctttt cttccgtcaa actataattc catctttact ggagtaacat
    20581 aatttggaaa ggcttctatt gtttttaata caatcgtata aaaagcgggg agacaactga
    20641 atgaagattt tagttttgta cataaatttt tgcttaatat agaagtcctg atatagataa
    20701 gatcaagtat ttatctataa ctggcctgat taattaaaaa actggaagga actgctcgat
    20761 catccttgag aactgctctt cttacttctg gtcaagattc tcgtggttgg tggataagga
    20821 tggttgataa gttcattgga ttagctagtc gaaaacaacc aggtgtaatt aatgtagaag
    20881 ttttaacaag gggatggagt gatttattca gtgataatca taagttcata aatagtaatt
    20941 gagatgtatg gttggaatac gttatacctg aacttgaact accaagattt ggtttagaat
    21001 ttgagaatcg atgtttataa agggagggca aaaatgcgtt tttttacaac ttttactatc
    21061 attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac agcattttta
    21121 ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt tggatttttt
    21181 ctattcacag ttattgtatt tatttggaga actccagcac caccattgcc agagattaca
    21241 tatggagaat ttccttttag acttgagtat gaattgaatg aagaattaca tgtgatagaa
    21301 gacacgttaa tagttgaatt tgatggattt ggaatgaacg aaggcatagg gagatatcgt
    21361 agatggacaa gtcgtttagc cagcggagaa gatttagtgt tgctgttaga agttagtgat
    21421 aataagcaaa tattttattt tccaggacct gctaattatt acatgggaga taggttgaat
    21481 ggttataatc atacttttcc tagtgcttct ttcattgaaa gggaaagagg cattactaga
    21541 agggatattt tacatgacaa agaattatta gaacaatttg gtccgcttga tcaaaataca
    21601 ataaatgaag aagaattgtt aaatcaatat aatataaggc tagtgaattg ggaaatatct
    21661 gaaccgattg ttaacaattt tggtgattag aaaacaatta cgataaatag aaacaagcca
    21721 aatcatcagc aagtaaaagc tgatga
//
LOCUS       DACDVT010000044        19639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDVT010000044 DACDVT010000000
VERSION     DACDVT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.044"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="IWL52_001674"
     CDS             complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="IWL52_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9778224.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            2146..3117
                     /locus_tag="IWL52_001675"
     CDS             2146..3117
                     /locus_tag="IWL52_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9778225.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3141..5333
                     /locus_tag="IWL52_001676"
     CDS             3141..5333
                     /locus_tag="IWL52_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9778226.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            5350..5826
                     /gene="ybeY"
                     /locus_tag="IWL52_001677"
     CDS             5350..5826
                     /gene="ybeY"
                     /locus_tag="IWL52_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9778227.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5804..6205
                     /locus_tag="IWL52_001678"
     CDS             5804..6205
                     /locus_tag="IWL52_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9778228.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6220..7119
                     /locus_tag="IWL52_001679"
     CDS             6220..7119
                     /locus_tag="IWL52_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9778229.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7262..8074
                     /gene="recO"
                     /locus_tag="IWL52_001680"
     CDS             7262..8074
                     /gene="recO"
                     /locus_tag="IWL52_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9778230.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8458..9375
                     /gene="glyQ"
                     /locus_tag="IWL52_001681"
     CDS             8458..9375
                     /gene="glyQ"
                     /locus_tag="IWL52_001681"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9778231.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            9377..11452
                     /locus_tag="IWL52_001682"
     CDS             9377..11452
                     /locus_tag="IWL52_001682"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9778232.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            11836..12615
                     /locus_tag="IWL52_001683"
     CDS             11836..12615
                     /locus_tag="IWL52_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778233.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            12656..13600
                     /locus_tag="IWL52_001684"
     CDS             12656..13600
                     /locus_tag="IWL52_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9778234.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13616..14395
                     /locus_tag="IWL52_001685"
     CDS             13616..14395
                     /locus_tag="IWL52_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9778235.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            14408..15106
                     /locus_tag="IWL52_001686"
     CDS             14408..15106
                     /locus_tag="IWL52_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9778236.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15134..15898
                     /locus_tag="IWL52_001687"
     CDS             15134..15898
                     /locus_tag="IWL52_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9778237.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            15911..17728
                     /locus_tag="IWL52_001688"
     CDS             15911..17728
                     /locus_tag="IWL52_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9778238.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            17742..18965
                     /locus_tag="IWL52_001689"
     CDS             17742..18965
                     /locus_tag="IWL52_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAP9778239.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            18979..>19639
                     /locus_tag="IWL52_001690"
     CDS             18979..>19639
                     /locus_tag="IWL52_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9778240.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KT"
BASE COUNT         6722 a         3519 c         4089 g         5309 t
ORIGIN      
        1 tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg tttgatttgg
       61 ttattcttct ggtaactcac ttgcttcttg tttatatcct gatacgcgat atacataata
      121 aaagagcaag ctgccagcgt aaccaagcaa tgttgggacg acccagccaa gacctaatgc
      181 agcaaaagga acataatgat gataaaatcc tagtaagctt gctacgacag tttgctgact
      241 cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag aaaagaacat
      301 cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg aaagtaagat
      361 taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt
      421 atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat
      481 ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat
      541 agcagtagtg aagacaccta atgtgaccat tacccctaag aagctagcac cgaagttacc
      601 taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata aagacatctt
      661 attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt ctaacgcaac
      721 gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa aaccaagatc
      781 acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat caaccgtgtt
      841 gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg cagcagattg
      901 gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta ataatgcaag
      961 aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat gtacagtaag
     1021 gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg aatcatattt
     1081 ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc
     1141 aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat accattttcc
     1201 gacaggttta gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc
     1261 taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga tccagtttga
     1321 tccagccatt tgacctaagt gtacaggaaa gatcagatta cctgacccga agaacatccc
     1381 gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg tttcttccat
     1441 aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca gaaactagaa
     1501 ataccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa aagccctttt
     1561 ccacttggga aaagggctct agattctaga acggtaccac cttttttcgc ttaaaaaagc
     1621 ttcttcattc acggacgctt ttcgataaag ttatccgcta gcctgataat ggaggcagcc
     1681 acaatgatct acgttttcga cattgactcg gaaatgatgt tcgtctattc gatttattgt
     1741 catttctcac ttacatgact cactgggaat aaatcactga atagcctact gttttcctcg
     1801 acgctttgct gtatatttat ttgttgacgt tataatacac ctgttttttt tgaaatgcaa
     1861 gaatatttca aatattttgt tttctttttt tgaaacaaga aaaataccca gagaatactt
     1921 gctaagttag acggaatact tgtcctctat cttatgcata gcttacatga tcagcttttt
     1981 ttattcaaac gagaccttta gagaatggaa gcggctagtt ttttaacgtt tcttggtact
     2041 tatggttaag caactctttt agaacctttt cttatttgct atatagtttc ttctgagtat
     2101 ggtaaaatag gaaagaatta atcgaaaaaa aggggatgtc tatttttgac ggaagagtct
     2161 agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt aggtgcacac
     2221 gacaagcata taaaactgat tgaggaaaca acggaaacaa ccatccatac acgtggagaa
     2281 atcattcaga tcattggtga gcaatcccag atttccttgg ctgcctcagt cattcggaca
     2341 ttacaggaat tgatcaaacg aggaatccac gtaagtacgc ccgatgttgt gacggcattg
     2401 aaaatggggc aaaaaggtac attagattat tttgttgaga tgtatgagga agagatcgtc
     2461 aaagaccgta atggaaaacc tatacgggta aaaaacagcg gccaaaagaa atatgtcgaa
     2521 tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg aaaaactttt
     2581 cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg
     2641 acgcggcctg ctgtggaagc aggtgaaaat ctaggctttc ttcccgggga tctaaaagaa
     2701 aaagtggatc cttatctgcg gcctgtttat gatgctttat accaaatttt tggattggat
     2761 catacgaacc gattgatgga acgaggcgta atcgaaatcg cacctttagc ttacatgcgg
     2821 gggcgaacat tggatgatgc attcgttatc ttggacgaag cacaaaacac gacagtcgct
     2881 cagatgaaaa tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt caatggggat
     2941 acaagtcaga tagacttgcc tcgagggacg acaagtggtc ttgttcatgc agaacgaaca
     3001 ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac
     3061 cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca agaataagct
     3121 ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaacttg
     3181 gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc ggcgttacat
     3241 ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagaaagta
     3301 tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga aaactggcag
     3361 cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac cagaacaatc
     3421 ggatcaagca actatttgaa ttgatcgata agacgaatga tgccatcaac aaatcttatg
     3481 acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact acagaagagc
     3541 ggatcgccgc gctgaagaaa aattttgaaa atgtgaatgc agaaaatgtt gtgttttatc
     3601 aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact gagattcata
     3661 ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa gttcgtgaaa
     3721 gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg aatgtcacac
     3781 cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg aatgacgtat
     3841 tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta caaccagtga
     3901 tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct tcagctatga
     3961 aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg ctaattgcca
     4021 tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt caattccaaa
     4081 atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca gtcagcatag
     4141 tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc ccgctgtttt
     4201 atcccgctgc ttttgcacca cttgttttaa gctttttctt gaatcgaaga gcagggatca
     4261 tggctggtat tttccaagca gtatccgcac ttttcatttt ttatgattcg attggcacga
     4321 actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca acgattgtca
     4381 gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt atttttcctg
     4441 tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat gcaacgactt
     4501 ggttaatgct cgtctgcggg ttcactggtg cactcttctc attcttgctg acgatcggtc
     4561 tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta aatgaactca
     4621 gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt acttatcatc
     4681 atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc ggacgttctt
     4741 tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat gctagctttt
     4801 tcgttgaaaa cttgccttca ggagcggaaa acccgcacaa tttcttattg ccagaagaca
     4861 gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa gattataata
     4921 tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta atgcgttatt
     4981 tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc cgttatcctg
     5041 gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg
     5101 cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc cataatctta
     5161 tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg aaagaaatcc
     5221 gaaaagtgga aaaatcatta atcagcggtt taagtagcac cttccattct cggatcaaat
     5281 atcctaaaat gaaaacagaa gttgagaaga taaaggaagt acaagaagaa tagaaagaag
     5341 cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga agagaaaatg
     5401 aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc agaggaaaca
     5461 gaaatgtctg ttactttcat ggacaatgcg gcaattcaag taatcaatcg tgattaccgt
     5521 ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa
     5581 atcccagtga tttttgaaga tggagagaat ccattacctc gtgtactagg agacatcatg
     5641 atctctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata tgatcgggaa
     5701 cttggctttt tagcggtaca tggatttttg catatcaacg ggtatgacca tatgacacca
     5761 gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta tggactcaaa
     5821 agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg ctgtccaagg
     5881 agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct
     5941 tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat ggctgctttt
     6001 agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa atgtagtgga
     6061 catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg
     6121 agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat ttgtccctaa
     6181 aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg
     6241 tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg
     6301 ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa aatccaagga
     6361 gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat ccataaacca
     6421 aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggat
     6481 gcagtcttat ttatgatcag tgctgatcaa aaacgtggcc ggggagacga ttttattatt
     6541 gaacgtctaa aaaacgtgca ttctcctgtt tatctgatta tcaataaaat cgataagatc
     6601 catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc ttttgcacaa
     6661 gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga tgtattagtc
     6721 agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa
     6781 tacttcatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg
     6841 cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat
     6901 atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat cggcaaaggc
     6961 ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga
     7021 gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc
     7081 tatctgcaag acttcggcta ccgaaaagac gagtactgat attctggact tcttataggt
     7141 taggtataga aataaactgg ataaaaatcc gtacgttgag aaaaacagcg gaagatcgaa
     7201 acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa
     7261 tatgagccag cttgtagaat cgaaaggctt gattttatct agccgtaatt acaaagaaaa
     7321 agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg
     7381 aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg aagcagtcta
     7441 tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc
     7501 gtttcggaac atccaagaag atatcttttt gagcggttat ggtacgtatt tgctaaatct
     7561 agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat ttacgcaaca
     7621 agctctgcag cggatggacc aaggagatga tgcagaaatc atcacgaata tctttgaagt
     7681 gcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga
     7741 aacaaaagga aaatttgact attcttccaa atatggcggt gtgatatgcg agaaacactg
     7801 gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt
     7861 ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa cgaagcaggc
     7921 aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc taaaaagcaa
     7981 aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag agaaaaatat
     8041 gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa
     8101 actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa tagatcgttc
     8161 actgagagga tctattatac aaaccagaaa actgcaaaat gaatacgtac attgaccaag
     8221 atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac ccaaacgatt
     8281 tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt cggaagtagg
     8341 gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac tttttgtcgt
     8401 tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg
     8461 agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg gtcatcaaac
     8521 ggctgcatgc tgatgcaagc ttatgataca gaaaaaggag caggtacgat gagcccatat
     8581 acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga gccatcccgc
     8641 cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt
     8701 caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga aagcttgcgt
     8761 cttttaggaa ttgatccatt agaacacgat attcgctttg ttgaagacaa ctgggagaac
     8821 ccatcgatgg gttgtgccgg tgtgggctgg gaagtatggc ttgatgggat ggagatcaca
     8881 caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac ttctgagatc
     8941 acttatggac tagaacgtct tgcttcttat atccaagaag ttgaaagtgt ttatgatctg
     9001 gaatgggctg atggcgtgaa atatggtgag atcttcaaac aaccagaata tgaacattct
     9061 aaatattctt ttgaagtaag cgaccaagac ctgctactaa gcaattttga tcgttttgaa
     9121 aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga ttatattttg
     9181 aaatgtagcc atacattcaa tttattagat gctcgcggtg cagtatccgt aacagagcgt
     9241 gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca
     9301 gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc agaaaacgtg
     9361 aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg aagaaatgcc
     9421 tgctcatgta gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa aatttttaga
     9481 tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc gcttagcaat
     9541 caaagtaaca aacattccag aacgtcaagc agacagcgaa gaagaagtca aaggaccagc
     9601 caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag gatttgtccg
     9661 cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg tggaatatgt
     9721 ctatgtaacg aaatacactc acggcaaaga agcaaaagaa gtattgacag gtttagcaga
     9781 tgtcattaaa agcctcactt tcccagtaac gatgcactgg gcagaatacg attttgaata
     9841 tattcgccct attcactgga tcgtggcatt gttagatgac gaaataatcc cgtttgagat
     9901 cttggatgtc aaaacagaca gagtttcaag aggtcatcgt ttcttaggcg aagatgtgac
     9961 atttacgcat gcaaaagaat acgaagaaaa attgaatgaa caatttgtta ttgcttcacc
    10021 gcaaaaaaga aaagaaatga tcatcacaca agcaaacgaa ttagcagaaa aaaatcactg
    10081 gatcattgat ctagatgaag atttactaga agaagtcaac aatctagtcg aatatccaac
    10141 tgtcttttct ggcgacttcg aagaaaaata tctatctgtt cctgaagaag tactagtcac
    10201 ttctatgaaa gaacatcaac gttatttcga agtacggaac gatcaaggta tgttgcttcc
    10261 tcattttatt tctgctcgta atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa
    10321 tcaaaaagta ttgactgctc gtttagaaga tgcggaattc ttttataatg aagacaagaa
    10381 agcttcaatc gaccaatacg ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg
    10441 tagcatctat gagaaaatgc aacgggtaca agtcatcgct cgcatcatag gcgaacaagt
    10501 aggactatct gaggaagaat tgatggatct gcaacgcgct gctgaaatct ataaatttga
    10561 tctagtaacc aacatggtag gagaattccc tgaactacaa gggatcatgg gagaaaaata
    10621 tgctctgctt aaaggcgaga aaccagccgt agcaacagcg atccgtgaac attacatgcc
    10681 tatatccagt gaaggagatt tacctgagac tacagtaggt tctgttttag cgattgctga
    10741 taaattagat agtatctttt ctttctttgc agttgggatg atcccaagtg gctcaaacga
    10801 tccatatgct ttgcgccgtc aagcatatgg tgtcgttcga atcatcgaat cgaaaaaatg
    10861 ggcctttcct ttgtctgttc tgcaagaaac gattagtgaa gttatcagta aagatacgga
    10921 tcgttttggc attggcttgt cagcgggaca acaacaggtg atcgacttta tcaaaggccg
    10981 tctgcgccaa ttattgacaa cgaagaatat ccgtcatgat gtcatcgaag ctgttttgaa
    11041 tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca aacaacatct
    11101 tgcagatgaa gacttcaaac cttcaatgga agcattgaca cgtgtcgtga acttagcgaa
    11161 aaaagcgcag cttgaaaaac aatcagaagt agaccctgag ctgttcgaaa acgacgcaga
    11221 gaaagaatta cacaaagcag tagaagcaat gaaagcaagt tttgctgacc aaacgatcca
    11281 cgaaaactac gaccagcttg tcgctcttcg tccactgatc gaaaattatt ttgatcaaac
    11341 gatggtcatg gctgatgaac cagcagtacg taagaaccga ctaacacaac ttagtcaaat
    11401 aactgccatg gctttgtcat tagcaagctt ggatcaaatc atcacaaaat agtcttttgt
    11461 ttgaaaaaca gagagcatgc acgaaacgtg ctgtgtctct gtttttttgt tataaatata
    11521 aaagctgtta ttaattctat tttaataatt tttgttattt tattctttgc tagaattttc
    11581 aaaacgctca tgaaaagtga ggaaataaaa atgaaaaaga ttaataataa tcttgttact
    11641 gttttattta cttttaaatt aggaatttcc ctagtcaaga aaactgtttg tggtatgatt
    11701 atatagggaa aacctgttta taaatatttc tatgaatata ttagtttaat tatgctttta
    11761 aaataaaaag cgctcattct gatgtgaatg aatcacatct ttatttatct atcatcaata
    11821 tttaggagtg aatctatgga aaagccgttc gtcaagttat tggctactgt ggcggtaggc
    11881 acaggcgcta ttgtgatttg cttgtttgga taccatctca ataatcagcg gcaacatcat
    11941 caacggatca attacgcaga atctgccatc accaatcaaa aagacaccgt gacctcttta
    12001 tcaaaagaag tagataagct gtacagtaca aaagaaaaaa ttttcctaaa tcctgaaata
    12061 acagaagaaa cagtttcaaa tttgtcacat aaactatctt ctatcaaact ttcagcagat
    12121 gattatgaca tcaaggaatc agaattacct aaagaagctg ctgcgatcca agaagaaaag
    12181 aaagcagtct taacacagtt agaggatgca gaatcaaaac tgaagatcca aacggctgtc
    12241 aataaattat ttactaaaaa cgtatcaaac tggcagcaag ctgtggatga tgtcatcatc
    12301 aaagaaaagt tggcaagtgc agatgtcgcc catgtacgtg aaaatatgag cttttttaag
    12361 gatagtgctt ggaaaacggt cgttatgcaa tatctaggat tcgctgatac ccaaattgca
    12421 caagtcacgc aactagatca gctattcgat acgatgctga aagacggtca agtaacagct
    12481 actgctactt acgatcaata tttgaccgca ctcagtctca tcgaacagat ccggaatgaa
    12541 aaaataagag ccgcatacgc gtcaaaagca gaaacagtcg ctcagcaaat gggctatagc
    12601 aatacgagct attaataagc gagaattcaa gcaaggctag caaacggagg aaactatggc
    12661 aaaacgaaga agaagatcaa gagggaaaaa gatatttcta attattttag caattcttgt
    12721 cgtattagta ttagcaatcg taggagtagg agcaaaatta tatctagatc tctcacattc
    12781 tattgaaaaa acttacgaat cagtggaacg aacccaagga gaacgaacaa aaccagttga
    12841 tctggacaaa caagaatctt tctctgttct attgatgggg attgatacgg gagatttagg
    12901 tcgggtcgat caaggaagat cagatacgat gatggttgcc acagtcagtc ctgaagacaa
    12961 acagacaacg attgtcagca ttcctcgtga tacgtatgtc gacatcgttg gacatgacac
    13021 aaaagacaag atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt
    13081 acaaaaatat gtagacatcc caatcgatca ctatgtttca atcaatatga aaggtctaaa
    13141 agaactcgtt gatgcagttg gtggtatcga agtcaataat gacctcacct tctcacaaga
    13201 tggctatgat tttacgatcg gtaaaatcac attgaacggg gatcaggcat tgtcttattc
    13261 tcgtatgcgt tatgaagatc caaatggtga ctacggcaga caagaacgtc aaagaaagat
    13321 cgtagaaggt gtagcgaaaa aagtgttgag tctgaacggt gtaagcaact atcaatccgt
    13381 tttgaatgct ctagagtcga atatgaaaac agacatgagc tttgatgaaa tgaaaaagat
    13441 tgctttagat tatcgggatg catttggtac gatcaagcaa gatcagctgc aaggagaagg
    13501 cttcatgcaa gacaatatct cataccaaaa agtcagcgat caagaacttg atcgtgtgaa
    13561 aaaagaatta aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga
    13621 agagacaatc agcttgaaag aaatttttga aacattacgc aaacacgtga taacaatcct
    13681 catctcaatg tttgctggct tggccatctc aggcatcgtg acgtttttca tcttgacgcc
    13741 aaaatacagc tcacaagccc aattgatcgt gacactgccg caatcagata ctgcaaatgt
    13801 caatgatgtc aatacgaacc tgcaaatgat caatacgtat aaggatatga tcgtcagtga
    13861 tctagtacta aatgaagtca aagatcgttt agaaacagaa gacaatgtaa aaatgactgc
    13921 tggacaaatc aaagatgcca tctccgtcaa tcagtcacaa aattcacaaa tgttctcgat
    13981 ccaagcaaca agcacaaatg cagtgacggc tcaacaaatc gctaatacaa cgtcacaagt
    14041 attccaagag aacgccaaag acgtgatgaa tgtcgataaa atctcgatca tctcaggtgc
    14101 agttgccaat acgacacctg tttcgccaaa taataaattg aatctagtga ttggcttagt
    14161 gttaggaatg atggtaggcg taggtctagc tttcttattt gaattactcg accggacagt
    14221 aaaagatgaa aaatatgtga cggatacatt gggcttcccg atcttaggga ctgtaccaga
    14281 aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa ggaccagctc cgacacaacc
    14341 aaaagctaca aacgataaaa cagcgccatc acgcagaagc cgctcaagag tctaggagga
    14401 aagaaaaatg gcacgaacac agaaacaaaa agcagcccaa actcatgcag tcagtttggt
    14461 cacactcaca aacccctctt caccaatcgc tgaacaatat cgtacgattc ggacgaatat
    14521 ccagtttgcc tccgcagctg gtcagcaaat taaaacgatt gttgtgactt catcaggacc
    14581 tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt gtttttgcca aatctggtca
    14641 acgggtgctg ctagtcgatg cagacttacg taagcccgtc atctataaaa cgttcaagtt
    14701 gaacaacgca agcggattaa gtaccgttct tagttcatca ggaagtgtag cagatgagat
    14761 ccaacgaaca ccagtcgaga atttgtcgat tctgccaagt ggacctaaac caccaaatcc
    14821 atcagaacta ctaagttcgc caagaatgga tcagatttta gcagaagcaa gacaactatt
    14881 tgatgtagtg atcttcgaca tgccgccagt agttgccgta acagatgcac agatcatgtc
    14941 ttccaaaacg gatggcacct tattggtcgt acgtgaaaat atgtcacgga aagaatcatt
    15001 gactaaagca aaagaattgc tggatatggt acaagcgcga gtccttggtg ttgtatacaa
    15061 tggggcagaa cacagcaaag atgctggcta ttactacggg aattaagaaa taaaaagaga
    15121 ggagacaaga gaaatgatcg atctacattg ccatatcctg ccaggaatcg atgacggagc
    15181 agaaaacata gatgccagta tcgccatggc tgaaaaagcg attcaacaag ggatcacaca
    15241 tattttatgc acaccccatc ataataatgg aaaatacagc aatgaaaaat cacaagtcat
    15301 ttctctagtc gcttcgttac aagcagaatt agagaaaaga caattgcctt tgacgttatt
    15361 ggaaggacaa gaagtacgta ttaccggaac actgatcgaa gacatcgacc gagatgaaat
    15421 tttatttaca gatttagatg atacatatct tctaatcgaa tttccgacac tggaagtgcc
    15481 gttatatgca gaacagctct tttttgagtt gttacaacta ggaaagacac cagtgatcgt
    15541 ccatccagaa cgcaacgcgc attttcgtaa agacccgaat catatgctgc cattcctaga
    15601 tatgggatgc ttggcccaac tcaccgcacc aagttatgtc ggtgtatttg gaaaagacat
    15661 ccagaaaaca gccaaaatga tggtcaaaca taatcttgtg cagatggctg cttctgatgc
    15721 ccatggggtc tctaagcgca ccttttactt aaaagagtgc tataagcaga ttgccaagga
    15781 ctttggtaag gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga
    15841 tgaaatcagt tatccagtct atgaagagat aaaaaagaag aagtttggtt tattttaggg
    15901 ggagtgtaag gtgtttgtat taacaagaag aagaaaaatt gccacattgg ttgttgtgga
    15961 cagtttgtta ttgatcgctg caaatattgc agcagctaaa ttcatgaagc cgtttgtagc
    16021 tataccaatg gaccttattt taatctctac cggtttgtcg attggttttt atttatttta
    16081 tggtagttta ttcaaagttt tcactcggat caatcgctat acaaatttaa gagagatcat
    16141 tgcaattttt ggttcactat ctgcaagtga cgtctcaagt atcctcatac tgctgttcat
    16201 taatcggcaa tacagtttac ggttagttat ctttgcttat ctgctttcgc tcttattgat
    16261 catcggtagc cgattgatct ggcgtatcta catcgaaaca aaaaatatga gatatgtatc
    16321 agcggattct gctaaaaata cattgatcgt tggtgctggc gaaggcgggc gtattttata
    16381 caacagcttt ctgggatcca aaacggctca agatatccaa gttgtcggat ttgtagatga
    16441 tgatccgaat aaaagaaata cgtatctttc aggaaaaaaa gtattaggtg cattaaaaga
    16501 tattccggaa ttgattgaaa aatatgacat tcaaatggta acgatcgcta tcccttcctt
    16561 atcaagaaaa aaattacgaa gaatcttcga gttagtcgaa tccgctcacg tgaaagtcaa
    16621 cacgatgcct tctatagaag aacttgcttc aggaaaaatt agcgtatcaa aattaaaaac
    16681 aattgacgta gtggatttac tgggaagaga tgaggtaaag ctggatattg aatccatcaa
    16741 agatcagatc acagacaaag tcattcttgt caccggcgca ggaggatcga tcggttcaga
    16801 aatctgtcgg caaatcattc aattcaatcc tgcaaagcta ttgcttttag gacatgggga
    16861 gaactcgatc tacttgattg accgtgagtt acgcacgcat catcaaaatt gtcctacaga
    16921 aatcgtaccg atcatcgcag atatccaaga tcgcgagaaa atcaatgaga tcatggaaca
    16981 atatcatcca gatatcgtct accatgcagc tgctcataaa catgtaccac tgatggaata
    17041 taatccaaaa gaagcagtca aaaacaatat tttcggtaca aaaaatgtag cagaagcagc
    17101 taaagcagca aaagtgaaaa acttcgtcat ggtatcgaca gataaagcta acaatcctcc
    17161 aaatgtcatg ggttcaacaa aacgaatcgc tgaaatgatc gtgactggat tgaatgaaga
    17221 aggctgtacg aaattctcag ctgtcagatt tgggaatgtc cttggttctc gcggtagtgt
    17281 gattccagta ttcagagaac aaattgctaa aggtggtcca atcactgtaa cagactttcg
    17341 tatgacccga tacttcatga cgattcctga agctagtcgt ttagttattc aatctggcgc
    17401 attagcaaaa ggtggcgaaa tttttattct agatatgagt gaaccagtga agattgtaga
    17461 cctagccaaa aacatgatcc gattgagcgg gtatagcgaa gacgaaatcg aaatcattga
    17521 aacaggtatc cgtccagggg aaaaactata tgaagaacta ctgttggata aggaaagaaa
    17581 tgatgaagca gtttatgaga aaatctttgt aggaaacatc aaaggttact caatacaaaa
    17641 agtgatggat tttgtcaaga gcctgccgca agacgatgaa caattggcta aagatatcgt
    17701 cacttttgca aatgcttcga ataaataggg gggaaatata aatgaataaa ttagaaaaac
    17761 gtcagatacc attttcaccg ccagatatta ctgatttaga aataaaaaac gttgtagaag
    17821 tattaaaatc tggatggata actacgggac caaagacaaa aaaattagaa aaaaaaatta
    17881 caaagtggtt aggaacttct aaagtagttt gtttaaattc acaaactgct gctgcagaaa
    17941 cagggttacg tttgctggga ataggagaaa atgatgaggt tatcgtccca gcttatactt
    18001 attctgccag tgcatcggtt gtatgccaca taggcgcgaa cctagtatta atagactgtc
    18061 aacaagattc tttggagatg gattacgatg cattagaagc agcaattacg gagaagacaa
    18121 aagtgattat tcctgttgat cttggtggta taccttgtga ctacgaccgt atttttgaga
    18181 ttgtggagaa aaaaagaaat ttatttcaac ctagtaatga gattcagaaa gcttatggtc
    18241 gtataattgt tatggcagat tctgctcatg catttggagc taattggcat gatcagatgg
    18301 ttggtaatgt tgctgacttg acaacatttt catttcatgc tgttaagaat tttacaactg
    18361 cagaaggtgg agctttagta tggaaagagc acgacaaact agataatgag gcaatgtatc
    18421 atcagtgcca gctgctttct ttgcatggac agtcaaaaga tgcacttact aaaacacagt
    18481 tgggtgcatg ggaatatgat attgtgggtc catggtataa atgtaatatg actgatgtta
    18541 ctgcatcaat tgggttggca caatttgaac gctatcctaa tattctgaaa agaagaaaga
    18601 atataattaa gaggtatgat gaagcctttc aacaactagg aattgaagtt cttatgcatt
    18661 atacggatga ttatagttct tcaggccatt tatatattac tagagtacct aacattagtt
    18721 ctgagcaaag aaatgaaata attataaaaa tggcagaaca agggattgct tgcaacgttc
    18781 actataaacc acttccaatg tttacagcat ataaaaatat ggggtttgat attaataact
    18841 atcctaatgc ctatgctcat tttgtaaatg agattacatt accattgcac acgaaactta
    18901 cagatgaaga agtagaatac gtaattaata actatagtaa gattgtaaag gatttggttg
    18961 gatgatagga gaggcaaaat gagaaagtgg gaagatttgc caaaagaaat gcagaatgat
    19021 gaaatacggt attattatga tatcctcagt aaaaaaaaat tcagcttagt ggtaaaaaga
    19081 gtatttgata ttttagtgtc tgcaactttg ttactgacac tttcatggtt attttttatt
    19141 cttgcaatag tgataaaaat tgattctcct ggacctgtat tttatagaca agaacgtatt
    19201 actcgttatg gaaaaaaatt taaaatttat aagtttcgta ctatggtaat aaatgcagat
    19261 aaagatggca ctcttgttac tgtgaaaaat gatgatcgta ttacaagagt aggaaaggta
    19321 atccgtaaat atagattaga tgaaattagt cagctaatta acgttttggt cggagatatg
    19381 tcttttgtag gaaccagacc agaagttgga aaatatgtaa agtcgtattc accattaatg
    19441 ttagccacat tattattacc agccgggatt acatcagaaa ctagtatttt atacaaagat
    19501 gaagataaat tgttgcatga taaaaataac atcgacaaaa cttatataga aaaaattctt
    19561 cccagtaaaa tgtattacaa tttgaaaagt atcagtgaat ttagttgtat gaatgatttg
    19621 aaaataatgt gtaaaacta
//
LOCUS       DACDVT010000045        19427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDVT010000045 DACDVT010000000
VERSION     DACDVT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.045"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWL52_001691"
     CDS             complement(451..1761)
                     /locus_tag="IWL52_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9778241.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWL52_001692"
     CDS             complement(1773..3191)
                     /locus_tag="IWL52_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9778242.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IWL52_001693"
     CDS             complement(3431..4999)
                     /locus_tag="IWL52_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9778243.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IWL52_001694"
     CDS             complement(5003..5413)
                     /locus_tag="IWL52_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9778244.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IWL52_001695"
     CDS             complement(5410..6333)
                     /locus_tag="IWL52_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9778245.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IWL52_001696"
     CDS             complement(6330..7208)
                     /locus_tag="IWL52_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9778246.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IWL52_001697"
     CDS             complement(7195..8532)
                     /locus_tag="IWL52_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9778247.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IWL52_001698"
     CDS             complement(8535..9638)
                     /locus_tag="IWL52_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9778248.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IWL52_001699"
     CDS             complement(9712..11220)
                     /locus_tag="IWL52_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9778249.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IWL52_001700"
     CDS             complement(11186..11878)
                     /locus_tag="IWL52_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9778250.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IWL52_001701"
     CDS             complement(11900..12874)
                     /locus_tag="IWL52_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9778251.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IWL52_001702"
     CDS             13174..14046
                     /locus_tag="IWL52_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9778252.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IWL52_001703"
     CDS             complement(14504..16702)
                     /locus_tag="IWL52_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9778253.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IWL52_001704"
     CDS             16934..17926
                     /locus_tag="IWL52_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9778254.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IWL52_001705"
     CDS             17986..19407
                     /locus_tag="IWL52_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9778255.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
BASE COUNT         5818 a         4012 c         3244 g         6353 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatga
//
LOCUS       DACDVT010000046        18928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDVT010000046 DACDVT010000000
VERSION     DACDVT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.046"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..462)
                     /locus_tag="IWL52_001706"
     CDS             complement(<1..462)
                     /locus_tag="IWL52_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAP9778256.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKT"
     gene            545..694
                     /locus_tag="IWL52_001707"
     CDS             545..694
                     /locus_tag="IWL52_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP9778257.1"
                     /translation="MKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPL
                     ENELR"
     gene            complement(1443..2056)
                     /locus_tag="IWL52_001708"
                     /pseudo
     CDS             complement(1443..2056)
                     /locus_tag="IWL52_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3277..3981)
                     /locus_tag="IWL52_001709"
     CDS             complement(3277..3981)
                     /locus_tag="IWL52_001709"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9778258.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            complement(3996..4865)
                     /locus_tag="IWL52_001710"
     CDS             complement(3996..4865)
                     /locus_tag="IWL52_001710"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778259.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            complement(4973..5989)
                     /locus_tag="IWL52_001711"
     CDS             complement(4973..5989)
                     /locus_tag="IWL52_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9778260.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            6179..8902
                     /locus_tag="IWL52_001712"
     CDS             6179..8902
                     /locus_tag="IWL52_001712"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9778261.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            9030..9452
                     /locus_tag="IWL52_001713"
     CDS             9030..9452
                     /locus_tag="IWL52_001713"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAP9778262.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            9470..9940
                     /locus_tag="IWL52_001714"
     CDS             9470..9940
                     /locus_tag="IWL52_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9778263.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            9964..10746
                     /locus_tag="IWL52_001715"
     CDS             9964..10746
                     /locus_tag="IWL52_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9778264.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            10736..11560
                     /locus_tag="IWL52_001716"
     CDS             10736..11560
                     /locus_tag="IWL52_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9778265.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            11595..12593
                     /locus_tag="IWL52_001717"
     CDS             11595..12593
                     /locus_tag="IWL52_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9778266.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            12610..13464
                     /locus_tag="IWL52_001718"
     CDS             12610..13464
                     /locus_tag="IWL52_001718"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778267.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(14038..15288)
                     /locus_tag="IWL52_001719"
                     /pseudo
     CDS             complement(14038..15288)
                     /locus_tag="IWL52_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            15805..15888
                     /locus_tag="IWL52_001720"
                     /pseudo
     CDS             15805..15888
                     /locus_tag="IWL52_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15977..17388
                     /locus_tag="IWL52_001721"
     CDS             join(15977..16532,16532..17388)
                     /locus_tag="IWL52_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9778268.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            17463..18746
                     /locus_tag="IWL52_001722"
                     /pseudo
     CDS             17463..18746
                     /locus_tag="IWL52_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         6228 a         3035 c         3642 g         6023 t
ORIGIN      
        1 tgtcttagta tataaaccca cactctgtaa ctttctaaag gcggagccaa gagaaggtgc
       61 tttatcggtc acaattgctt tcggctcacc aaactgttta tggagtcgtt ttaagaaagc
      121 ataggctgct tgcgtatccc gtttctttcg taaccagata tctaaggtta agccgtccgc
      181 atcaattgca cgataaagat aatgccaacg tcccttaatt ttgatatagg tttcgtccat
      241 tttccatgaa tagaaggatt gtctattttt cttcttccaa agataataga ggactttgct
      301 gtactcttgc acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatataa
      361 caattcctga acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat
      421 aatgacgtct tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc
      481 ttttttctca attttacact aaaatagatt ttttggaaaa ctttgcaaca gaaccatttc
      541 taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca aacatccaga
      601 agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa tgttagatga
      661 tgagttgatt ccacttgaaa atgaattgag gtaaatgaga agagttaaga aaattgtacg
      721 attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct tttatttgtt
      781 cacattttat aacgaagtat ttaagacagc tattgaataa tgtttcctcc acctttttca
      841 ttcatttacc ccaccccgtt gttatatgac cgtgtttcga gagaaaccaa taaaaagcat
      901 aggctaacta ggggttcctt tttcttttga ttttttggaa tccgcttaag tgaaatgtat
      961 ctgaaatgaa acagagataa agtgattttt tgctgtaatg acaaggtttt agctgtttca
     1021 gataccatgt aacccaaaat agtttattga gaaaatagga gagtatttga tactaactag
     1081 taggaaaaaa tgagataaaa agtaaatgga atcatttcag gtaatctcaa ataaaaaata
     1141 tacaggcctg tggcacttta cctacgggac tgtatatttt ttactctcag cgggcttgtt
     1201 ttgactccgt gtttgactcc gtcgtattga ttttaaatga tattcgatga ccaattaata
     1261 ggtcgttttt gaaatttttg tctctatatg actcagtttg ataattattg atagatcgat
     1321 ttgggtctat atcgaacaag ggaagattgc aaaagtcgaa gcttaaagga ttgatattaa
     1381 aggattaaga ggtaaaaagg tatccccata atatcctctg actccgtttt tgacctcgct
     1441 tatcatccgt tcatgtaaga atcaaataat tcgactgttt cattttgccg attttttgat
     1501 actttaatat aaatgtccat gattgtttta gaagaagaat gtcctagcct atcttgtaca
     1561 tcttttactt gagcacctgc ttcaaagaga agggtagcat gagtctttct gaagccatgg
     1621 actttgatta tcttcatatc tgggaacttc cgtgctactt tatccatgac atggttaggg
     1681 tagcctttgt aaaggaagtc ccttttttta ttccctttga tagtgctgat tacaggtata
     1741 tcatctatat tatctatccg aatccctcta gaaaggttct tttatgttac agaacgtttc
     1801 cattttttag aatacgtaag gttccattat caattgaaat tactcccttt gatttgtctg
     1861 tttttgtagg attaatacat agagagccct cggatagttc tactaacgtt ttgttgaagc
     1921 agatagtttg attatcgaaa tctatgtctc tacatgtcag tccgagtatt tctccttttc
     1981 gttcccctgt ataagaagct attctaaaga atactaaagc acgctcatct tttaacttat
     2041 gcaaacattc aaaaaacaga tatttcacat atatctaaaa ttcggagatt gaaagaatga
     2101 atttatctaa gaattttcac taatgttgcg aatataatat cagaaaattt cgaaataaat
     2161 tttgttaaaa attagaaaaa tccgaatact tagtagtgga taaatttttt tcaggaatag
     2221 tacgttttat taaaatgaaa actaatccga catcaacttt tttatcgaag caaacatcaa
     2281 gcggtaaaaa gacctcttta gtttgaaaag tgttgctttc atattgattt aaattttggt
     2341 taagtaggta aattaagaaa tgatcagtgt aagtatttcc tgatttagcg caccttgttc
     2401 actttgaaaa tacaggtttt tgatggacag attttgcttc aaatgtttaa agaacatgcc
     2461 actgtacttg gtacaggtcg gcgcccccat ttggagttgc gtcagatcga gtcgtgacga
     2521 ttcattttgt acaaattaga atttaaaatt gaatacatga cgcgcacatt ttcccagaga
     2581 gccatattct gtaattaatc acagcaatta gtgattatgc acaatctaaa aaactgattt
     2641 ataataaaaa tattattcaa ataaataata ataactgccc gcatgatgtt gacagttaca
     2701 atagcacata gatagactgg caaagatggt tcttaatctt tcactttggt cagaattctt
     2761 gggttatcct tccagtgaaa gtcagcagaa aatccttaat gcttacaaat aatttttgcg
     2821 aaataggttt tattggtaaa ggactcaatc atgaatatga attactttta tctggtaaat
     2881 ttggaaaaat tttgcaaaga aataaaaatt agacaaattt gctacacata atcaaagatt
     2941 aaaaaagttt ttaaaatata gttgtttgaa tggggatatg aattagagaa aattggttga
     3001 ttcaacgggt gctatcattt ttgatagcgt catatgtgaa gtaagcacac ctaatgaaaa
     3061 aggttcaggc ttgctttgaa gacttattaa caactatgtc gactatcctt gacttagggt
     3121 agaggagtac ctccacacac cttaattaga tgataatgtt tggactgtag gctacttagc
     3181 ggattaagat aaagaggaag tagaaaaaga gccttgcgga aagacacttt atctgttatc
     3241 cgcgaggctc tttatacatt taagcatatt taaaaattat ttggaaacag tactttcaaa
     3301 ctttgtctta taagcagggt acttagatat ttcatattca aacggtttct gatttttagc
     3361 aaatccaata cttgtaaaga caaagactgg ctcatttttt tctaaatcaa gataaaggtt
     3421 ttctaaatct gttggcggtg taacttcaat tgagcgtttg gcataagcta atctaattcc
     3481 cctactttct aatactgaga acagggattc tttggcaaaa tcatgttcaa caatttcagg
     3541 taaaacagat aggggaatgc aactttttgc aatacaagtt ggatagtcat cgatgtatcg
     3601 aattctttcc aagacaagca ctaaagtatt ctgctcaatc tccaaacatt cactaattgt
     3661 tgaatcagca ggtatgatgg tttgttggat aatcttagaa ctgattttct ttccttgctg
     3721 aaccgcatct tggaagaatc cgataaaaga gtcgagttcg tggtcaacat atttttctga
     3781 agctggtttc actagatacc cgcgaccttc atttttagaa ataattcctt cgtcttctaa
     3841 agaagctaaa gctttccgaa ttgttggacg actcaccgaa aattcctggg ccaaatctct
     3901 ttccgaaggc aataattcag atttccgatc agtggtgata tacacttttc gaatttgttc
     3961 ttttactttg tcaaaaatca tagtaatcca tccttttacc aaggtattaa cattatatta
     4021 ccattttttt gacttaatat cccagatttt ccttcaatta attccttcgc cgctaattga
     4081 ccccacaaat agccaatata tcggtcataa aaagttggag atgatccacg ctgaagatac
     4141 gcaagattta agtatttggt ttcttttggt aaatttttgg taataaattc aatgattttt
     4201 tgtttgttag atataccttc tggtaaaagc aatataggcg agtagaactc cgactcaaat
     4261 tttttacata ttaagtgtaa gtattccttg tcgtctctct caacccaata agtattattt
     4321 gcaatggaag cgtactctgg aagagcaaag cagcctcggc ccatgacttc cattataata
     4381 atacgatcat gagcattagc agtagaagtt aaatcatcga ttgatcggat tatagtgttt
     4441 aaggcgctat cgaagccaag tgtataatcg gtattgggga aatcgttatc aatagtccct
     4501 gggatgacag atacttgtaa ttctgattca tttttcaata gttcagctcc tttcaaggaa
     4561 ccgttacctc caataacaat taagttgtcg atctccatct tatttaggtt ttcaataata
     4621 attttccgac tttcgagatc tctcattttc tcaaatctgg ctgttccaat aagtgttcca
     4681 gctttgaata cctcaacttc ttttagagaa actgaggcaa tacattcctt aaatattccg
     4741 gcataaccat tcttaaaaaa gactacttcc gaatctggtt cgctatcaat tgtttttttt
     4801 attgctaata aagctgaatt cattccggga gagtcgccac cactacagat aactgcgatt
     4861 ttcattttag atcctcctaa aaaaggtgac tgcgattcta ttctcccatt agagaaaatg
     4921 aagcaatcaa atgggcaatg gagtcgcggt cacctttttc atatttttat gattagtatt
     4981 ccatttgctt ataataacgg cgagtagtta aagggtggtt acgttcttct tctagaaaag
     5041 cactaattcg ttcaaagaca gtagctaaga cgactgggga aataattttt cgcgtgtctt
     5101 ccgaaatgcc aggtaagtca tagtcctttg tatcaaccgt tacgtgaaca tctgtaaact
     5161 taggaacgaa attcttcacg cgttctgtga gaggtcgaga atcatcttct ccttctgtaa
     5221 tgatgacact tacacccgct tcaattaatt caagagttcc atggaagaag tctgctgcat
     5281 gaacaggacg agtcttaatc cattgcatct cttctaaaat acacatgcca tagtaatatg
     5341 cttccgcaaa gctatcacca gcaccggtta aaatatgata tgaagtatct ttgtgttttt
     5401 ttgcaaaaga ttttgccatt ggttccgctt gctctttagc gtgcactaag gcttcaggta
     5461 ttttttttaa atcctcaata taggtattgt attgattgat atcgatttca tttctttgtt
     5521 tcattaagaa gagagcaatc agcagtcctt gaaggtaata attttctgaa gatgtatcat
     5581 ctaaaacgtc gttatagata gaatatgttg cagctttttc taggggcgtt ttaggacttc
     5641 ctaccaaagc taatgttttg acgttcattt cattacagta ttcgactaac tcaagtgttt
     5701 cttttgttgt accagataaa gatgggataa caaccaaaga tttgttagaa aatgttttat
     5761 tgggatgaat cataaattcg gcagcgataa cacgatgaaa agggaaagtg ctacgagttc
     5821 tcgcgaattc gattgttgaa tcaaaaagaa taccaactcc tccagaccca ataaagtaaa
     5881 tttcttctaa gtcagatgcg atggattcta aaatattgga aatttgttca tttgttgcaa
     5941 cggcaccagc ttgagtcttt aaaaatcttt cttgatcaaa attaaacata aaaatctctc
     6001 ctttgattac caatggtaat attttgtttg cataaataaa ataccacagg taataaaaag
     6061 tgtcaaatat tttttaggaa aaataccata ggtattgtta aagtggaaag atgtttataa
     6121 agaatattga attttatagt gaaaatcatt tattattaag taaaggaggt gggttcattt
     6181 gaaaacgaaa attttaaaag aattaaatga tctacttaac agctatgatt ttccacaata
     6241 ttttccagaa gaatgtaacg cgcaatatat tgcccaaaaa tttcaaataa aaagaaatac
     6301 ggctagtaag catctaaacg aactaatgga acagaaaaaa gttgtgaaaa tcaatggtcg
     6361 tcctgttctt ttttttccag tccatacaat tgaacggtat atatcacatt cattagatag
     6421 ttttgagttt tcttcaattg attcgtttag agaaaaaatt tcaaagaaaa aagcagatca
     6481 tttttttgat gatttaattg gtgcaaaaca aagtttaaaa gaagcggttc aacaagggat
     6541 atcagcaatg aaatatccag gtggattacc aataatgtat tttggatcgt ccggagttgg
     6601 gaagagttac ttagtggaaa aactagctga gtattgccga caaactggta ttattcatga
     6661 gaatgctccg ttcatcgagt taaactgcgc tcagtatttt aataatccag agttattaac
     6721 aagtcagttg tttggccata tgaagggatc gtttaccggc gccgattctg accatattgg
     6781 tataatagaa gcagcagacg gtggaatcgt ctttcttgac gaaattcatc gtttaccacc
     6841 agaaggacaa gagaaacttt ttattcatat ggataaaggc gtttttcgaa gagttggaga
     6901 tagtggtcct tggcgtttat cacatgttag atatattttt gcgacaacgg aacaggaaga
     6961 aaattttttt ctagaaactt ttcgacgaag aattccgatt gtttgtactt tgcctaatta
     7021 ccaagataga gaaattagcg aacgaaaaga gattgtcttc catttattag agaaagagtc
     7081 tactctgatc aataaagaaa tattagtttc tgaagccgcc cttagtttcc tgattcgctt
     7141 agatttgtat ggaaacattg gtgagctgga tggactaata aaacaaacta ttgcgaataa
     7201 actatttagt ggaagcaata aaaaatatat cgaaattcat atttctgatt tacctagcag
     7261 gtacttgcta acagaaaata ttgagttaaa aaaccaaatt gcgagccaac ggttctatat
     7321 ctttagtaat ggtaactatg ttcatgattg tgacgagact tcaactgtaa ctacaaattt
     7381 acgaggtttg attcatcgtg tttctaaata ttattcagag tgtcatgatg tgaatagaaa
     7441 tagtgaagag tttcgacgtt ttgtgaagga ccaaatggca gaatttaatg atcgttttat
     7501 ttaccaaaaa aattcagaag tcttagttaa gtattattta ccggaaatcc aaaatattgt
     7561 tcaaaaaatc tctcccagtc tttttgttca cctttctggc aatacggtcc aagctatagc
     7621 tacctatctc gctattaggg atcaatgggg gtatgggaaa gagaatgctt cagcgaatga
     7681 aattgaaaag ttatatgaaa acctaaagtg taactatttg acggatttaa atgtattctt
     7741 actgatcttc gagaacgccc tggatacaac attgactatt tgggataaac tgtatatcag
     7801 tttgtgtgtt attagtgaga ccggaaatga agcgaaagac attaacgcaa ttattttagc
     7861 ccatggatat gcgacagcta gtagtattgc taatgttgcc aatcgtttgg ttaatcagtt
     7921 tgtcatagat tctattgata tgcctctaga tatcagcttt agtgaagtaa ttgacagatt
     7981 acttcactat atagagtatc gaaagcctaa agatggacta gtgattttcg ttgatatggg
     8041 aagtttagct caaattaaga ctgaaattga acaagtaatc gaagttccaa caatgattat
     8101 taataatgtt acaaccgaaa tggctattga aaccgcacaa ctgattcaaa gtacgagtga
     8161 tatccaaaag gttgtaaaaa aattgccgta ttctcagttt gaaaagcaag ttctatatcc
     8221 catcaaaatt agaaaacgta caatcgttgt ttcctgcaat actggtttgg gtacctccat
     8281 aaaaattaaa gaaatgatgg aaaataattt gtcgaaagac ttgggcattg aatttctgcc
     8341 atacgaaaat gaaactctta gagatactca gcaattagag tttctcatta aaacaaagga
     8401 tatcattgcc attatcggaa cagatgatcc gcgtgtgtcg aacattccct atatttctct
     8461 tgaagaatta tttgatggtg aaattggcaa actctataaa gttcttttga gagtctatgg
     8521 aaaagagatt gctgaggcaa ttaatcaaaa tattgttaaa aatagctcat taaaccgctt
     8581 gattgacagt cttactattt tagatgccga aaaagttgtc aatcaaattg aggaaagttt
     8641 aaaagattat caaatgaggg tggggaaaac gttatcatca ttgtccaaaa ttaatcttta
     8701 tgttcatatc agctgtatga ttgagcgact aattcgtaat cagggagtaa gtagttatcc
     8761 agcaatagat cagtttctta agcgatacga aactgaggtt gcttctattc aaaaggcact
     8821 tagtaagatt gagaaacttt ataatattaa gattcctatg gaagagattg catatgtgtg
     8881 caacatcatt gtaagttatt gataaaataa agtgacacta ttcacagcga tggaaatagt
     8941 gtcactttat tttttgtacg ccctttcatt taagtggtga cagtaattgg cacagtattt
     9001 gctaataaca aagcgaaagg agttcgggta tgagacaaat attatgtatg agccacggtc
     9061 ctctagcaaa aggaatgatt gaaactttga gtattatcgt aggagatttg gataatattg
     9121 attttcgatg tgcctatgtc gatggaaata atgacgtaga aatgatcatc gatgattatc
     9181 taaaggaatc tatgggaaat gagacgattg ttgtaaccga catttttggt ggaagcatta
     9241 ataatgagtg gctaagacgg ttgccagatc tttctaaagt ctatttgatt tctggaatga
     9301 atttaagtct tttagtggat ttatatttaa aattccaatt agtttcatct gatcagattg
     9361 aagaaattat tgatcatgca attcaagcat cttctggttc gattaagttt tgtaatcagc
     9421 tagattatga gcaaacggtt gatgactttt aaataaagga gggaagagaa tgattaaatt
     9481 agtgaggatc gatcatcgtt tgatacacgg tcaagttgct ttaacttgga cgaacgcaat
     9541 cagtgcggat tgcattttag tagcgaacga tgaaattttg aatgatccaa ttcaaaagac
     9601 aactttgaaa ttagccgcac cacataacgt gaaggttgtt atcaaatcaa ttcaagattc
     9661 gattgatgca attaatagtg ggaagacaga taaatacaaa ctatttgtag ttaccaaaac
     9721 catttccgat gctaaacggt tggcaaatgg ggttgaacaa attaaatctg taaatatcgg
     9781 tagtgttaag gaaactagtg gcagtagcgc tttgacgcat acaatttttg taacagaaga
     9841 ggaacgagca gaacttaaag gaatggtctc agagggcatt gaagtgttcg ctcaacaagt
     9901 tccaacagat aaggcaatag atttaaaaaa atctttgtaa taaaaaaata agggggtaat
     9961 ttcatggata gtctcttagt acaagcaatt ttgcttggtc tcttgactgc gttcatcgta
    10021 attgactgga tgacaggtac gaataactta tcaagaccaa tcgtatcttc cgtctttgtt
    10081 ggtttaatta tgggcgatct taagatgggg attatgatgg gggcaacact ggagttagca
    10141 tttatcggag caataacaat cggagcggca cgtccgcctg atacggtatc tggtgggatt
    10201 ttagggactg cgtttgctat tgcaactggc aagggagcag aatttgcgtt gaccttggca
    10261 ttcccaatcg ctgcgctatt cttattagtt gataatctac taacattgct tgttctacca
    10321 atgattgcta gaaaggcaga tagatatgct gaaaagggcg aactaaaaaa tgtgtcgaaa
    10381 atgcatgttt taggttttat cttagtaaaa agtttaccac gggctttatt tgtcggattc
    10441 tcattttact taggaagtcc tgttatggaa cgaatcttaa atgctattcc aacttttgtc
    10501 caagatggga ttaacattgc tgcaggcatt atgccagcac taggttttgc tattttattg
    10561 caaatgcttc ttaagaaaga tgtctttgta tatttcgtaa ttggttttgc actctttagc
    10621 tacttacatg tgccgattct ggggattgca atcttcggcg catgtctagc ttatattata
    10681 gtaaacatgg aaaccaaagc tgcaatgggt tcaagagggg aggagatggt tgaagatgac
    10741 ttctagtggg aaagctttaa ctaaagatga tttaacaaaa atttgtattc ggtcatgtgc
    10801 attagaagca tcatggaatt ttgagcgcca acaacatatg ggctttgctt ttgctatgat
    10861 gccagcgtta gaaaaaattt atagtggcaa tgaagaagaa aagcaaaaag cagctcaaag
    10921 acatctagag ttttttaaca cagctccgcc attatcaaca ttcataatgg gaattgtggc
    10981 ttctatggaa gaagaaaatg ccaaaaatcc tgatgacttt gatacttcta caatcacttc
    11041 tattaaaact gcattgatgg gaccattagc tggtattggt gattcaatgt tttggggtac
    11101 cttattagtt atcggtatcg gtgtaggtgc ttcattagca aaagaaggca atattttggg
    11161 tccattttta tttttactaa tcttcaatgt ccctaatttt cttctccgtt actttggagt
    11221 tatgcaggga tataactttg gtacaaagct attagagaac attgaatcct ctggcgtcat
    11281 gaaaacgttg actcatgctg cctcaatttt gggtcttatg gtaattgggg gcatgtctgg
    11341 aagtatggta tcaatcaaag tagctggttc tattggacaa ggtgatgaag caacaaaaat
    11401 tcaaagtatc attgatggta ttttaccaaa tctgctgtca gtattatgtg tatttctgat
    11461 ttactggatg cttaaaaaag gctttaaaac aacttacgtg ttattcggaa tctttatctt
    11521 tggtatcata gcagccttct tcggattttt gacaacttaa ttgaataatc taaataattg
    11581 taaggagaga actaatgtta aactataatg caaaagaaat gaaaaaaaaa ggcaaacgga
    11641 tttacggtat tcgtgaagat gtagaaaaaa ttgcaaagca agcttatgat tctggaatta
    11701 gtaatatttt ctttactgct tctggtggat cggtagccgt aatgcaacca tttgagtatg
    11761 tattgatgac tgaatcaagt attcctgttt actctatgac tgcagctgag cttgtcttaa
    11821 tgggacataa acagttgaat gaaaaatctt tagtaatctt atactcaaaa tctggagata
    11881 cgaaagaaac tattaaggca gctgaatatt taaaagaacg aaacgtgcca accgttggta
    11941 ttgtaagtaa agaagataca ccgttagagg ctctatcaac ttatgcaatc aattacctag
    12001 acggccgccc tcaagaaatg ccactttatt tcttaattat gtctatcatg aatttgaatg
    12061 gggaatttcc tgaatacact cgttttgctg atgaactgaa aaacttacca acggctctga
    12121 attcagttag agaacaggct gatgaaaaag ggaagaagta tgccgaagaa tttaaagaag
    12181 atccttatca aatttggatt ggtagcggga atttatgggg aatcacctac tcttattcaa
    12241 tgtgtgtgct tgaggaatct caatggttgc gtacaaagtc tgtctcatca ccagagtttt
    12301 tccatggtac cctcgagtta gtcgaaaaag gagtttgtgt aacacttttg aaaactgagg
    12361 gggaaacacg gcttttagat gctcgagttg aaaaatttgc ggtccaatat acagataaat
    12421 ttaatgtttt tgatactaag gattatgagc taccaggtat tagtgacgaa tttcgaaagt
    12481 acttttgtgc tccagttatg tgggcagccc taggtcgcgt cagtgttcat cttgaagata
    12541 ttcggaacca ctctttggat aaacgtcgtt attaccgagt agttgaatac taaaagtagg
    12601 tgattaatta tgacagcgta tgtgattgga tttggcgata atgttgttga ttactatgag
    12661 aatttgaatc aaaaatttcc agggggaaat gcagttaatt ttgctgtgaa tgcaaaaaag
    12721 agtggtgtta aagcatacta tgtcggtagt ctatcatctg atcctgatgg cgtgttatta
    12781 caacacgcca tcagttctga aggtgttgat atctctttct cggaagttat tgacgagcct
    12841 actgagcaag cacatgtaac tattaaagat ggagataggg agtttatagg tggcgtgcgt
    12901 ggctcccgat tgacacctgc tctctcagca gaacttattg atttgatgaa gaacagttca
    12961 ttagttcata ctagttgcca tagtcgaaca gaaacaaaaa ttaaagaatt aaaaaaatat
    13021 gatgttaaag tttcttttga tttttctgac ttagataaat atagaacgca tgaatacttg
    13081 tcagatgttt gtccgtcaat ttatattgca cagttttcag tcgctacaga tgatcaagag
    13141 gaaattaatc gcttaattag tgaatgcaag gactttgagg ttccgtatct tctttttacg
    13201 cgtggtagta agagccctgt ttttgttgac actcgagaga ataagactta tgaaggattc
    13261 attcgcaaag ttgagtcacc tcatgataca atgggcgcag gtgatgcata ctttgcagct
    13321 tttgcaagta attttgtctt atctaaacgt gaaaaatcaa tgatagaaca tgttattgat
    13381 tgttttcaca aaggtgcaaa ccttgcatat gaaacattga tgattgatgg ctcctttggt
    13441 catgctacac ctattaaccc ttagctttgt gctttgggct tttattgctg gaatttgtat
    13501 agtagactca tcataactat atttttgtat gtgaaaaggc aagaggctat gatgaaaata
    13561 acactggcta attgcatgag ggagcatatc gttgttaaag cgtcattgat agtatgaata
    13621 aatatctcga taatattata ttgttattta cttaatttat tgattagtca agcaaagata
    13681 atcttctgtt acttaacgag actagtaggt acaagcactt catgttatgt acgacccaaa
    13741 aatattgctg ttgattattt gctgaaatta ttgcgagctt caatttattt ttaaaggact
    13801 tggtaggctg cgtgatgaaa taagacttgg aaggctgtct aaaccaatat atagtagact
    13861 aaatagtaaa aaagagcatg gtaattgtct ctttgtcaat aagtctaatg gtaataagac
    13921 aagttggaga ttgagaatat ttttattgtg taacctattt tgaagcgtac ttaactagcc
    13981 ccggccaaaa tcaaattttt tcggggtata tcaagggttt gcgtcaataa cagaatgtta
    14041 tttatctatc acgttccttg gtggcgtgta gagttgatgg ttacgtagta gcgtatctac
    14101 cagacgcgta aattttcttg cggttaagac gtgtgctcgt ttgtgtttat ttttgggacc
    14161 tctttacatt tgctttgata aaaagcttta tattgaggaa gatatttttt tacagagttg
    14221 gaagcttcaa ctaagtagta tctaaaatag cgactccttt gttttgtcag aggtgtattt
    14281 tgtgaccgat agttaacaga ttgtttgcct ttccaacata agcctgcgta ttttgccaat
    14341 tttgtttgat cttcaaatcg ttcaatttga ccgatttcag cgattaatcc agcggcgtgt
    14401 acctttccaa tacctggaac actagtcaaa tattgatatt ctggaataac gacaacaaat
    14461 tgctcggttg ctttatccaa gtctttgatg ttcttttcaa gcgcacgaat ttcacataca
    14521 aaaacattta gaacgacatt gatggattct tatgcaagac tatctaaccg ataactcacg
    14581 gcaattgcac gttgaatcga tttggcattt ttttctggat ctttaaagcg acatttcctt
    14641 tttcttttag taagtcacag aaagcttcca aaggaagcac agccaattca tctagcgtga
    14701 agtcttctgt cacaagagta atgacagtgg cactatagag acttgtcgat tcatcgcgca
    14761 tttatcgagt aagcgtattg catttgtacg ttagattttc cagaaagtgt tgttttgtac
    14821 gagtgaagtt gcttaatgag tcgatagcga gtgcgtgtta aacgctgtaa agccacgtac
    14881 ttctcttctt tgataggaga agttgtgaac cgttgaatac gcaataaatc agcaattctt
    14941 aacgcgttgt ttcgatcagt cgtatcttcc tcaaacatgc gactgaattg tttcatccga
    15001 aaaggatttt caatcctcac aagtgcattg agttttttta gctctgcatc ttcatggaaa
    15061 aacatcgaag gatgaaagct gtacatggac gtttaattcc atctcaataa cgatttgacc
    15121 gaagtgattg ttttcattga acacaagaat cagttctcgt gtcaatggag ccctttcaat
    15181 gtcattagca acagaatttt cagtcaatat agatagctga tcgtcgtctg ttaggaagca
    15241 aacgtctaat ttttcagaac taacgtcatt accaacaaat aatttcatgg aataaaaacc
    15301 tattttcgat ataaattcta gaaaatgctt cgatactgtg ggattccaca gaaactcact
    15361 gtgtggtcac gacctcgcct aatagaatac ggtaataaaa gagctaatga gttgatagta
    15421 aacacagcgc tttggataac agacttatga aggtagacaa agctacagga ctgattagca
    15481 aattctcgtt gaatcctttc catacatcca ttgtactctg agaaatcaca caataacgaa
    15541 ataaattccg gaaggaatat gttattcaaa aaaatttgtg ttttatagat actttaaaag
    15601 aaacttatta ggcaaaatat attttacgaa gactgatgag ttgtgcaaaa tcaaatctga
    15661 gtcagaatga acttcattct agctcagatt tttgtttaat gacttcagtc agtttcttgc
    15721 attacgtgaa tgaaaatggg actggtgatg tccaaaccct aaaaagttag accaaaatct
    15781 aacttcaggg gggatggtat ttttatggga aaatatagct ttgaattcaa gtcaacaatc
    15841 gttcaagagt atcttgaagg agaaggtgga ttaggatatt tagcaaaaat acaaggagtg
    15901 agatcaaaaa aaggtacacg gatggatcaa tcaaaagtta gaccaaaaaa tctaactttt
    15961 gggggtcgtt atttgtatgg caaaatatcc ttttgaatta aaactaaaaa tcgttaaaga
    16021 atatcttagt ggcgtgggcg aatatcggca tcttgccaac aaatacggta tttcaagcaa
    16081 gtctcaagta agtgattggg tgaatgccta tcgtgaatac ggagaggaag gtctattgaa
    16141 aaagcatcaa aatcagaaat attctgttca atttaagtta aatgcgctag agttatatca
    16201 aacaagtgag atgtcctatc gagaagttgc cagcacttta gaaatgaaca accctgcgct
    16261 tattgccaat tggatgcgta atttcagaga agcaggtatt gaggggctct caaaagagaa
    16321 agggcgacca cttactttga ctagaaaaaa agaaaacaaa aaagaaaata caacaactga
    16381 agaacgcgag cgtatcaagg cattagaaaa gcaagtccga tcccttcaaa ttgagaatgc
    16441 ctttttaaaa gagttgagga agctgcgaaa acaggaagcc cagcaaagac gcaagaatca
    16501 atcgcacgaa tcatcgccag cctccgagga tcattcaaac tagttgagct tcttgaaaca
    16561 ttgaaatttc ctaaagcaac ctatatgtat tggcagaaac ggtttgacag accaaaccca
    16621 gaccaagaaa ttgaagaaaa aatgcttgaa attcgcaagg agcataaaga ctatggctgt
    16681 ctgcgactaa aaaaagaatt agagaatcaa gggattcatg tcaataaaaa gaagattcaa
    16741 cgtctgataa aaaaactagg tatcgaagta aagtcttaca ctcgaaaatc tcgacggtac
    16801 aattcttatc atgggaaggt tggcactgtc gccaaaaatc gtattcatcg tcggttttat
    16861 acaaatatct gtcaccaaaa aattacaact gataccacag agtttaagta ttatgaagta
    16921 gacaccacag gtgtcgttcg tcagaaaaag ctgtatttag atcctttcat ggatttctac
    16981 aatagtgaaa ttctatctta tcggatttct gaaaaaccaa acgcactagc cataatggaa
    17041 ggattagaag aagcaatcca gatgacaaat gattgtccat ttcgtagaac cttccattca
    17101 gatcaaggat gggcctatca aatgaatgct tataagaata aattgaagca gcataaaatc
    17161 ttccagagta tgtctcgtaa agggaattgt ttagataact cacctatgga aaacttcttt
    17221 ggtttattaa agcaagagat ttttcatgga gaagtctatc ggagtttaga tgaattgaaa
    17281 acaaaaattg accaatatat ctattattac aaccataaac gcatcaaaaa gaaattaaat
    17341 tggcgaagtc cggttcagtt tcgagaagct gtaaaaacag ctgtctaaca gttaatttta
    17401 tcgtacaaaa aaggagtgga tttctccact ccataaaagt ctaacttttt ggggtcgcta
    17461 caaatgctta tcgagaattt ggagaagaag gcctcttgcg taagcgaaat aagcgaagtt
    17521 attctgttca attcaagata gatgcgatag agttataaaa aacgggtgag ctatcctatc
    17581 gtgaagtggc gaatcattta gggataaacc aacctgcgtt agttgtcaat tggatgaaaa
    17641 tttttgttca aaaggattgc acgggctctc aaaaatgaaa gggtcctcct ccattttgtc
    17701 taagaaagaa taaaaggaag aaacaagcaa actgacgcct aaagagcata atcgtatcaa
    17761 agaattggaa aaataagtgc gtttccttca gattgagaat acctttttaa aagaattgag
    17821 gaagcttaga aaacaggaag tctaacaaag acgaacgaat caatcgcacg aatcatcgac
    17881 aacctccgag gatcattcaa attagttgag cttcttgaaa ccttgaaatt tcctgaatta
    17941 acctttatgt attggcaaag acgatttgat cgaagaaatt cagatcaaga aatcgaagag
    18001 gaaatgctta aaataagaag tcaatataaa gattatggct atttacgtat gacaaaggaa
    18061 ttgtgggacc gtgggttcca tgttaacaag aaaaaagtac aacgtttgat tcgaaagctg
    18121 agtattgaag tacgatcatt tacgagaaaa tatcgccgct ataatactta tcgtggaaga
    18181 atcggcatga ttacaaaaaa tcgaattcat cgacgctttt acacaagcaa ctgtcatcaa
    18241 aaggttacaa cagatacgac agaactaaaa tattttgaaa tggataaata cggtgccaat
    18301 cgtgaaaaga agctgtatct tgatccattt ttagatatgt acaacagtga aattttattt
    18361 tatcgcattt ctgaaaagcc aagtgcgttg gctatcatgg aagctttaga ggaagctatc
    18421 tgcgcaacgg aatgtatcta tcgtcgaact tttcactcca aaaaggatgg acctaccaaa
    18481 tgaaagctta tagtcataag ttgaaagaac ataagatctt ccaaagtatg tcccgtaagg
    18541 agacctgctt agataattcg atcattgaga acttccttag tctattaaag caggaaattt
    18601 attatggaag agactatcgt atctttgaag aattgaagca agccattgat ggctatattc
    18661 attactacaa ccatgagcgt atgaaaagga aactaaactg gcagagtctg gtgcaattta
    18721 gagtaatagc attcaaagct gtctaaaatc atcaagaaaa gcaaagaaaa acggagtgaa
    18781 ttttctccac tccgaaaaca tctatctttt ttaggtcact acagaatcaa ttctgattca
    18841 tcagacccat aaattataga tcctaataat ttataccatg ctctttttac tcatatttac
    18901 atttatcttt gggtatttta tggtttaa
//
LOCUS       DACDVT010000047        18315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDVT010000047 DACDVT010000000
VERSION     DACDVT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.047"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(219..1727)
                     /locus_tag="IWL52_001723"
     CDS             complement(219..1727)
                     /locus_tag="IWL52_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9778269.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1886..2077
                     /locus_tag="IWL52_001724"
     CDS             1886..2077
                     /locus_tag="IWL52_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778270.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2280..3197
                     /locus_tag="IWL52_001725"
     CDS             2280..3197
                     /locus_tag="IWL52_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9778271.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3190..4263
                     /locus_tag="IWL52_001726"
     CDS             3190..4263
                     /locus_tag="IWL52_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778272.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4411..4929
                     /locus_tag="IWL52_001727"
     CDS             4411..4929
                     /locus_tag="IWL52_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778273.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4910..5302
                     /locus_tag="IWL52_001728"
     CDS             4910..5302
                     /locus_tag="IWL52_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9778274.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5378..6313
                     /locus_tag="IWL52_001729"
     CDS             5378..6313
                     /locus_tag="IWL52_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9778275.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6446..7225
                     /locus_tag="IWL52_001730"
     CDS             6446..7225
                     /locus_tag="IWL52_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9778276.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7218..7535
                     /locus_tag="IWL52_001731"
     CDS             7218..7535
                     /locus_tag="IWL52_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9778277.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7779..9389
                     /locus_tag="IWL52_001732"
     CDS             7779..9389
                     /locus_tag="IWL52_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9778278.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9546..10394
                     /locus_tag="IWL52_001733"
     CDS             9546..10394
                     /locus_tag="IWL52_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9778279.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10625..11104
                     /locus_tag="IWL52_001734"
     CDS             10625..11104
                     /locus_tag="IWL52_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778280.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            11137..13365
                     /locus_tag="IWL52_001735"
     CDS             11137..13365
                     /locus_tag="IWL52_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9778281.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13494..15137
                     /locus_tag="IWL52_001736"
     CDS             13494..15137
                     /locus_tag="IWL52_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9778282.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15210..15755)
                     /locus_tag="IWL52_001737"
     CDS             complement(15210..15755)
                     /locus_tag="IWL52_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9778283.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15778..16263)
                     /locus_tag="IWL52_001738"
     CDS             complement(15778..16263)
                     /locus_tag="IWL52_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9778284.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16253..17065)
                     /locus_tag="IWL52_001739"
     CDS             complement(16253..17065)
                     /locus_tag="IWL52_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9778285.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17204..>18315)
                     /locus_tag="IWL52_001740"
     CDS             complement(17204..>18315)
                     /locus_tag="IWL52_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9778286.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5963 a         3324 c         3651 g         5377 t
ORIGIN      
        1 aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca
       61 ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa
      121 aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc
      181 taatagtttc gttttcaaca tttataagta aactttttct attggtaata tagattttcg
      241 gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca
      301 tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca
      361 taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca
      421 ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt
      481 tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact
      541 gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg
      601 acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct
      661 tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa
      721 ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt
      781 ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg
      841 acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat
      901 tgataaacag attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc
      961 tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg
     1021 atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt
     1081 ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct
     1141 ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca
     1201 ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca
     1261 tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag
     1321 accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta
     1381 tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc atcgattaac
     1441 cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata
     1501 tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg
     1561 attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc
     1621 ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct
     1681 gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct
     1741 taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc
     1801 tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga
     1861 tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa
     1921 acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga
     1981 acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg
     2041 cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga
     2101 ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt
     2161 ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa
     2221 aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta
     2281 tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg
     2341 atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga
     2401 atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata
     2461 tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa
     2521 atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg
     2581 aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg
     2641 gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg
     2701 ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg
     2761 acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt
     2821 tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat
     2881 tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac
     2941 tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc
     3001 aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa
     3061 aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc
     3121 atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag
     3181 gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa
     3241 gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca
     3301 gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg
     3361 aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta
     3421 gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa
     3481 ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa
     3541 tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg
     3601 atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca
     3661 aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga
     3721 caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa
     3781 tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg
     3841 gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg
     3901 atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt
     3961 aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa
     4021 ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat
     4081 atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg
     4141 ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga
     4201 tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc
     4261 tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg
     4321 gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa
     4381 cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc
     4441 ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag
     4501 ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt
     4561 gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc
     4621 ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa
     4681 acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac
     4741 ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat
     4801 taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt
     4861 gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat
     4921 aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac
     4981 ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat
     5041 cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga
     5101 tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac
     5161 ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga
     5221 cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat
     5281 tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata
     5341 attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg
     5401 aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt
     5461 cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga
     5521 ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga
     5581 gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat
     5641 gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc
     5701 atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat
     5761 cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact
     5821 ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga
     5881 agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc
     5941 aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg
     6001 agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc
     6061 aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga
     6121 tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt
     6181 tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca
     6241 gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta
     6301 ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg
     6361 tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt
     6421 tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc
     6481 tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt
     6541 attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt
     6601 ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac
     6661 gacattattg atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc
     6721 cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga
     6781 tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa
     6841 ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa
     6901 ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat
     6961 gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt
     7021 ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc
     7081 agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa
     7141 agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga
     7201 acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca
     7261 tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat
     7321 cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta
     7381 aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa
     7441 tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag
     7501 gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga
     7561 gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct
     7621 ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt
     7681 ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa
     7741 gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag
     7801 gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga
     7861 ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata
     7921 atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg
     7981 cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga
     8041 tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt
     8101 atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac
     8161 tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag
     8221 gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg
     8281 ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa
     8341 ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga
     8401 ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga
     8461 tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa
     8521 cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa
     8581 cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct
     8641 tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag
     8701 gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac
     8761 aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat
     8821 tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg
     8881 aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga
     8941 tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag
     9001 aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg
     9061 ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta
     9121 tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt
     9181 tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc
     9241 gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg
     9301 gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg
     9361 ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa
     9421 aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat
     9481 ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta
     9541 aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga
     9601 accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg
     9661 aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc
     9721 ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga
     9781 aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca
     9841 cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga
     9901 tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc
     9961 agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc
    10021 tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc
    10081 tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt
    10141 tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta
    10201 gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag
    10261 aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa
    10321 ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca
    10381 tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt
    10441 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
    10501 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
    10561 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
    10621 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
    10681 taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc
    10741 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
    10801 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
    10861 agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
    10921 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
    10981 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
    11041 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
    11101 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
    11161 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
    11221 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
    11281 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
    11341 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
    11401 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
    11461 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
    11521 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
    11581 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
    11641 cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc
    11701 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
    11761 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
    11821 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
    11881 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
    11941 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
    12001 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
    12061 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
    12121 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
    12181 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc
    12241 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
    12301 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
    12361 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
    12421 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
    12481 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
    12541 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
    12601 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
    12661 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
    12721 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
    12781 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
    12841 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
    12901 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
    12961 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
    13021 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
    13081 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
    13141 ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
    13201 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
    13261 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
    13321 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
    13381 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
    13441 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
    13501 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
    13561 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
    13621 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
    13681 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
    13741 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
    13801 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
    13861 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
    13921 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
    13981 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
    14041 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
    14101 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
    14161 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
    14221 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
    14281 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
    14341 aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa
    14401 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
    14461 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
    14521 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
    14581 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
    14641 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
    14701 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
    14761 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
    14821 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
    14881 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
    14941 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
    15001 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
    15061 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
    15121 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
    15181 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
    15241 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
    15301 acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg
    15361 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
    15421 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
    15481 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
    15541 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
    15601 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
    15661 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
    15721 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
    15781 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
    15841 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
    15901 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
    15961 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
    16021 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
    16081 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
    16141 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
    16201 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
    16261 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
    16321 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
    16381 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
    16441 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
    16501 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
    16561 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
    16621 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
    16681 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
    16741 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
    16801 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
    16861 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
    16921 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
    16981 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
    17041 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
    17101 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
    17161 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
    17221 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
    17281 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
    17341 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
    17401 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
    17461 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
    17521 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
    17581 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
    17641 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
    17701 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
    17761 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
    17821 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
    17881 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
    17941 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
    18001 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
    18061 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
    18121 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
    18181 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
    18241 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
    18301 aataaagaat aggaa
//
LOCUS       DACDVT010000048        18062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDVT010000048 DACDVT010000000
VERSION     DACDVT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.048"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(120..485)
                     /locus_tag="IWL52_001741"
     CDS             complement(120..485)
                     /locus_tag="IWL52_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778287.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            693..1175
                     /locus_tag="IWL52_001742"
     CDS             693..1175
                     /locus_tag="IWL52_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778288.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1372..2262
                     /gene="pstS"
                     /locus_tag="IWL52_001743"
     CDS             1372..2262
                     /gene="pstS"
                     /locus_tag="IWL52_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9778289.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2549..3823)
                     /locus_tag="IWL52_001744"
     CDS             complement(2549..3823)
                     /locus_tag="IWL52_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9778290.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4096..6567
                     /locus_tag="IWL52_001745"
     CDS             4096..6567
                     /locus_tag="IWL52_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9778291.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6670..8406)
                     /locus_tag="IWL52_001746"
     CDS             complement(6670..8406)
                     /locus_tag="IWL52_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9778292.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8403..9107)
                     /locus_tag="IWL52_001747"
     CDS             complement(8403..9107)
                     /locus_tag="IWL52_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9778293.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9238..9741
                     /locus_tag="IWL52_001748"
     CDS             9238..9741
                     /locus_tag="IWL52_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9778294.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9701..10039
                     /locus_tag="IWL52_001749"
     CDS             9701..10039
                     /locus_tag="IWL52_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9778295.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10060..11478
                     /gene="ffh"
                     /locus_tag="IWL52_001750"
     CDS             10060..11478
                     /gene="ffh"
                     /locus_tag="IWL52_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9778296.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11590..12168
                     /locus_tag="IWL52_001751"
     CDS             11590..12168
                     /locus_tag="IWL52_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9778297.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12172..12693
                     /locus_tag="IWL52_001752"
     CDS             12172..12693
                     /locus_tag="IWL52_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9778298.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12772..13326)
                     /locus_tag="IWL52_001753"
     CDS             complement(12772..13326)
                     /locus_tag="IWL52_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9778299.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13579..13854
                     /gene="rpsP"
                     /locus_tag="IWL52_001754"
     CDS             13579..13854
                     /gene="rpsP"
                     /locus_tag="IWL52_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9778300.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13866..14117
                     /locus_tag="IWL52_001755"
     CDS             13866..14117
                     /locus_tag="IWL52_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9778301.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14242..15033
                     /locus_tag="IWL52_001756"
     CDS             14242..15033
                     /locus_tag="IWL52_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778302.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15203..15724
                     /gene="rimM"
                     /locus_tag="IWL52_001757"
     CDS             15203..15724
                     /gene="rimM"
                     /locus_tag="IWL52_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9778303.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15714..16475
                     /gene="trmD"
                     /locus_tag="IWL52_001758"
     CDS             15714..16475
                     /gene="trmD"
                     /locus_tag="IWL52_001758"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9778304.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16611..16958
                     /gene="rplS"
                     /locus_tag="IWL52_001759"
     CDS             16611..16958
                     /gene="rplS"
                     /locus_tag="IWL52_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9778305.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            17216..17509
                     /locus_tag="IWL52_001760"
     CDS             17216..17509
                     /locus_tag="IWL52_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9778306.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5999 a         3224 c         3686 g         5153 t
ORIGIN      
        1 ttggagagaa gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga
       61 aaaacatata tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt
      121 tatggatgcc gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt
      181 ggaactaagt aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag
      241 aagtaacgcc attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata
      301 ttggcaatga ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga
      361 gataaaaata tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag
      421 gcgaaatgca cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt
      481 atcatctttc atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa
      541 tcctgcaata atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc
      601 attatctcta aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat
      661 gatgagagaa aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc
      721 tttatttatt tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg
      781 ttgtacaagg tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga
      841 attttccagc tttgattgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat
      901 ctctgatcaa tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat
      961 ctatgttttc tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat
     1021 tgaaggattt agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag
     1081 taattgcctc atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa
     1141 gtgaacgatc tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaaacat
     1201 atgagctgcg taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg
     1261 aaaaacgttt acatgatttt tacataactt aactatattt ttacagcaac catacgaggg
     1321 atttacagtc gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa
     1381 gtattgttta ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga
     1441 aatacagatg gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc
     1501 gttgcggtag gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta
     1561 caagaaaatc caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc
     1621 caagtcgaag ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac
     1681 ggagttgatg cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc
     1741 gtcaataaag atacagatgt caaagatatc accaaacagg aattgattga tatctttaca
     1801 ggaaaaatca ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac
     1861 cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc
     1921 cctgtgcagt cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca
     1981 ccaggagcaa tcagctattt agctttctca tatctcgatg attccacaca agcactaagt
     2041 atcgacggtg tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca
     2101 tatgaacata tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac
     2161 atgatgacag aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca
     2221 atgatggaag tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc
     2281 acggtagtcg ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct
     2341 ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
     2401 ctgattgaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtaacttttt
     2461 ttggctcttc gtcaaatcgt gttggtgaag acaaatttga ataaaactta gtccgatatg
     2521 cggcgcgttc tgtgcgccgc atattttttt agttttcaaa gatcagacct tgttgtaaat
     2581 agatccgtgc ccgaaagtta taaaagttgc gataaccaaa cgcaattcgt ttaatcagct
     2641 tgatcttgtt attcagcccc tcagtgacgc cgtttgagtg ggacacctta aaggcatttg
     2701 aaatacccga ttggtagcaa tggaagatcg tgaatttctt tttaaacgac tgaggtaacg
     2761 tagatgaaat tgttttaagg gtttcttgaa agccttccgt acctcgttta acgaaataat
     2821 agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc ttcgttgtag cttaacagct
     2881 catcaacgat ttctgtttga ctaattggtc gtttaaacag tcgatgataa tgccgtttga
     2941 gtggctccca tgtcgtggaa tctttgagta agagtttcca atatcgcttg agccgacggt
     3001 acttcttctg attttcagag tgtgaggttc gaaaacgatt catgatctgg acacgtaatt
     3061 gattgaagct gcgattgatg tgttggacaa tatgaaagcg gtcagttacg atctccgcat
     3121 ttggaaagac tgttttaagc aactgacaat agctggcatt catgtccatc actaggtact
     3181 tgacctttaa acgggccttt ctaggaaagc gcaggaagta ttgagttagt ttgtaaagtc
     3241 tgcgatcttc aaggatcgta aatagctgat tggttgtccc atcaacacaa ataaaactca
     3301 tggaaccaga aacagaattc attgatttga actcatccat acacaacacc gtcggaagga
     3361 agttcatacg tggctggtaa tgcttcgttg cttcacgcaa gatccgtgtc accgtcccat
     3421 ccgagacgaa gtacttctta gcgattagtt tacgtgattg attttcgtac agttccatga
     3481 gaatcgcctg cttcagttcc tttgttagtt gatgatgttc atcaactaag tccgttttcg
     3541 cagaaaaggt ccttccacaa tcatggcaga gaaaacgggt acgttttaat tctaaaatag
     3601 ttaacttttc tttgactttc aacatctgag tgcgtgtcgt ataggaaccg ttgcggataa
     3661 tacgaaccga tcgacagtgt tcacaatagg aagaagagtc atccaatttt cctttaataa
     3721 actgcgcttg aacgccttta tattcttttt caatcagcca ttcttcatca aaaaataaat
     3781 gtttatctgt taatccgagt aattttctag tatgattatc cattgagaga ctcctttggt
     3841 aggatgacgt tttggtcgac taaattttac caaaagtgag tctctttttg agttgaaacg
     3901 aagaaaaagt gttagtagaa aatcgccttc gattttccac caacacaaaa tattatagaa
     3961 cccttttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat gtgaggaaca
     4021 ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga aaacgaaatc
     4081 aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca
     4141 caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg attaacggca
     4201 gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga tcccacattc
     4261 aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt gatctttctt
     4321 atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt tttaccagtt
     4381 atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa gatcttggac
     4441 gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gagacgggat agaacaaaca
     4501 atagatatag aagaccttgt aatagatgac attgttattt tagagactgg atatcaaatt
     4561 ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta atgaggcttt attgacaggc
     4621 gaagcagatg aaataaccaa agaaataggt gatccattaa tgtccggcag tttcattgtt
     4681 tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat ttcccagctg
     4741 acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc ttctcttaat
     4801 caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct tttttctcaa
     4861 agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg
     4921 atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc attaagtgcg
     4981 actaaactag caaagcaaag agttctttta cacaatatga aaagcattga gacattggca
     5041 agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa aatgtctgtt
     5101 caaaaaatga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca attggaagca
     5161 tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga agctgttaaa
     5221 gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt ttcttctgtc
     5281 caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc gcccgaaatg
     5341 gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc
     5401 tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt attgacagaa
     5461 gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga agcaagagcc
     5521 acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg agataatccg
     5581 ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca gtacgttgat
     5641 gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac agtttttgga
     5701 cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga taaagcgtaa caatacagta
     5761 gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga ctgcagtata
     5821 gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt acttgattct
     5881 gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa caatattgag
     5941 cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc actgttttca
     6001 gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat tagtttgttt
     6061 actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg
     6121 aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga tatgatcgta
     6181 gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga tgtttctaca
     6241 gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg
     6301 ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt tttttcggtc
     6361 atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact tttgctgttg
     6421 agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt ggtagaatat
     6481 ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagc taaaaaattt
     6541 ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt aaaaaagaac
     6601 aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg cacgagcagt
     6661 tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc caagatgact
     6721 gtctacagta atcgtaccat taaggagttg cacgtagtct tttacaatcg ctaaccctaa
     6781 tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat
     6841 ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa acgatagatc
     6901 atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt ggacagcatt
     6961 ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa atggttcgtt
     7021 ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat aattttgaac
     7081 gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat tctcgccatc
     7141 ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat ctttttgcat
     7201 gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc
     7261 ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt tgccaacgaa
     7321 atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca cccctagaat
     7381 ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg tctgccgacc
     7441 ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt gatgaatcag
     7501 ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac
     7561 cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat cagcatcatc
     7621 aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct cttctgtggt
     7681 agtacacgcc cgatacatct tactcatttg ttcgctcaac gcattaatcg tctgatacaa
     7741 ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat tttgcaccat
     7801 tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga tcagataata
     7861 gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa
     7921 actctcagtt ctcggtaaaa aaacgcttgt cggttctgcg atacgtaaaa tggcttctaa
     7981 ctggccactt tttttgactg gtaatgcgac atataaaaga tcctcattca aagtagtact
     8041 tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt cgggacggga
     8101 gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc cagtagcatc
     8161 cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag ttttgttttg
     8221 cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt tttttgtcag
     8281 gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca attgccagcc
     8341 accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa tttttttcca
     8401 tttcatcgtt tgggttcctg aaacttatag ccaaatcctc taatagtcac caagtacttt
     8461 gggtgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac atctacgatt
     8521 cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc tcggtcgatc
     8581 acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg tgttagttca
     8641 atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat ttgtccaatc
     8701 gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat tcgtctgaat
     8761 attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa atagtcgtca
     8821 gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt cagcatcaaa
     8881 atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc atccatcgtt
     8941 ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa ttctaatcct
     9001 tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt gaacgtcaag
     9061 agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca aatctccttt
     9121 aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc tattttacaa
     9181 aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga gcagtcaatg
     9241 attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca gaaaaaagaa
     9301 gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg tccagaagtg
     9361 atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa tggattcgat
     9421 gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac ggaagcgtac
     9481 aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga tctagtcatc
     9541 atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacggaag tttttccgga
     9601 ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa aatgactgtg
     9661 ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg aaaagaccaa
     9721 tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac aaatgaacta
     9781 catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag aatacgaagt
     9841 aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg aggattatga
     9901 aaaaaaactc catctttttt ctaattatat tgttcgagaa caggtattgg aaaatatgac
     9961 tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg tacagcaaat
    10021 tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga aagcttaact
    10081 gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc agaagctgac
    10141 gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt taacttgcaa
    10201 gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga agtgctggaa
    10261 agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac gatcacactt
    10321 ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat catgatggct
    10381 ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta tttaaagaaa
    10441 aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc tgcgatcgac
    10501 caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg aactgaagtc
    10561 agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa aaacgactat
    10621 gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga tgagttgaaa
    10681 caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc catgactggt
    10741 caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac aggggtcgtc
    10801 atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg ctcagtgaca
    10861 ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga agtattccac
    10921 ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt gattgaaaaa
    10981 gcgcagcaag actacgacga gaaaaaagcg gaagaacttg cacagaaaat gcgggaaaat
    11041 tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat gggcccactg
    11101 gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga aaacgtcaag
    11161 gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac gccagcagaa
    11221 agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc cggttctgga
    11281 aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag aaaaatgatg
    11341 cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt tggttcaggc
    11401 gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa taagaagaaa
    11461 aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa atgtctactg
    11521 ccggagcttt tctatcagca atttttattc tttactaata caattactct aaacgaagag
    11581 gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca atatcatgat
    11641 actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt gatgcttgat
    11701 attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc attcgatgaa
    11761 gcatatgatc gattcgatat caaaaaagtc gcaaattatg gagaaacaaa aatcagtgaa
    11821 ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc cgtccaaaac
    11881 gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta tctttggtcg
    11941 ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt cccaacaaca
    12001 agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa atttatcgga
    12061 agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca ccttgacagt
    12121 tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag catgcgaaaa
    12181 atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac ggatgatctt
    12241 caatggcttt ttgacactcc aacaggtaag caaactactt atgaagcttt ttatgaaaca
    12301 atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata tttggagacc
    12361 gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca attacaattg
    12421 ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct acaaatgaca
    12481 gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct attggaacat
    12541 caaatgattg atgaatggta tatccagatt acccctgtat tattagggga cgggattcct
    12601 ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac acgattcggt
    12661 gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa aaggcaacta
    12721 tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt tttactctgt
    12781 taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac ttaatgttcc
    12841 ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat atgcttccgg
    12901 ttgctgcaag gttggcacat tcaaaaagac aagtgattgt cgtagatggt ggtttgcgcc
    12961 aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat gatcccaaac
    13021 actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg ctggcacaga
    13081 acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt cctcacgaaa
    13141 acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt aaaatggcag
    13201 atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg caaaagcttc
    13261 tgctactttt ttgttgtaag aatcttttct taaactacca ataaaaaatc caattttttt
    13321 tgtcatacga tttttcctcc ttcaaactat ctataataat cttacaaaag ataagtaact
    13381 ggagcaaata aaaacactta ctgacaaaag ctacatagaa gatagattac ttatttttaa
    13441 caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat cgaatttaat caagctgtaa
    13501 agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact acatcttgtg aataaaacaa
    13561 tggaggtgga aataaataat ggcagttaaa atccgtttaa aacgtatggg ttctaaaaag
    13621 agtccgtttt accgtatcgt cgtagctgat tcacgttctc ctcgtgatgg acgtttcatc
    13681 gaaactgtag gtacttacaa tcctttgaaa gaccctgcag aagtagtttt aaaagaagac
    13741 ttagttcttg actggttatc aaaaggtgca caaccttctg acactgtacg taacatcctt
    13801 tcaaaagaag gcgttatgca aaaacaccac gaagctaaat tctcaaagaa ataaggtgac
    13861 cggatatgaa agatttaagc gacttagtct taacaatcgt tcgtccgcta gtcacttatc
    13921 ctgatcaagt caagttagac gtcgtagagt cgaaagactt ttacgagtac aatctaacag
    13981 tggctcctga agatattggc cgtatcattg gaaaacaagg gcgtgttgct aaagcaatcc
    14041 gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa agtgcgtttg aatatcatcg
    14101 atgataagga aaagtaaata aacgtgctgt actcgaattt tcgagtgcag cttttttgtt
    14161 ttttttatta attgaagaaa tgattatttg gcaaaacaag caagaaagcg tatccttata
    14221 ggaggaggga gtgaacagcc aatgagaaag aaaatgttat ttattccatt tgctctattg
    14281 ttaagcgctt gtagttcggt tactcaagaa tcagacaata cacatctatc aagcagtata
    14341 tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa cggcttccac cactgaacaa
    14401 acaatggcta cctcaaataa tgagaaaaag actgctttag accagttgaa agaacaacag
    14461 ccaaatgttc ccatgcctct agatgtacca gtctcttcag gctatctcaa tatcgcagcg
    14521 acacatacaa agcaaggtta ttctatttta tattacagaa cagatcgtcc cctcgggttg
    14581 aatgctgacg aattgaatca agaaacgcct attgcaacct atctatatca atatgggttt
    14641 gcttctagtc aagaaacaat ccaagttttg cagccatttg agatcgacac aaatggtcag
    14701 caagtggact taggttctcg aatcacaggg tatcagcaag gtgcagcagg ttcaagtttt
    14761 cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg ggaataatat agaaggccaa
    14821 gatcccttac tattagctaa agaaatcgta gcttatttgg aagaaaacag tctaccagca
    14881 cctgaacaat ttggtaaaat tactgttgat atgggtgata cgacgaatcg agccgtagaa
    14941 gtaagctggc aagaaccgaa aaatgcctat acgatcactc atcaagatcc tatgtctgcg
    15001 ttgaagatgg cagtttctat gaagaggttg tgaaatcagc gcgctgatct gagcccatca
    15061 tttaactcca agtcacagga agtaatcaaa aatagcacgc cgcatttttg cataattttg
    15121 gcttaatacc ttaaaagaaa actagaattg cctcttcaca catgctaaaa taaaacagac
    15181 aaaaaagaaa tggggatata agttgactga atacttgaat gtaggaaaaa tcgtaaacac
    15241 acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg gatttcccag aagaacgtta
    15301 caaaaaagga acggtgctaa cactattcca agacggaaaa gcacctgtgg aattaacagt
    15361 caaaagtcat cgcaaacata aaaattttga cttattgagt tttgaaggac atccttctat
    15421 caatgatgtg gaaaaatatc gggatggcat attaaaagtg tcaaaagaca actctgtaga
    15481 tctagctgaa aatgaattct attaccatga gatcatcggt ctgcatgtat tagaagatgg
    15541 aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt gccaatgatg tttgggtcgt
    15601 tcaacgtccg aaaaaagctg atatattgct tccatatatc gcttctgtcg tcaaagaagt
    15661 cgatttagaa gctggagtgg tccgtgtaga aattccagaa ggactgattg acgatgaaga
    15721 ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc tatgggggaa tccatcatcg
    15781 gtaaggctgt agataaagga ttattagata tcaatatctc taacttccgc gattattcag
    15841 ataacaaaca tcagacagta gatgattatc catacggcgg tggagcaggt atgctgctca
    15901 aagtccagcc catctatgat aacatccaag ccattgaaaa agctgcaccg gatgtcaaaa
    15961 aaagagtaat tcttcttgac ccagctggta aacgattcga tcagaaaatg gcagaagaat
    16021 tttcacaaga agaacactta gtattcattt gtggtcatta tgagggatat gatgaacgta
    16081 tccgcacatt agtgacagat gaagtttcct taggagatta tgtccttact gggggagaat
    16141 taggtgcgat ggtcatgatc gatgcaacag ttcggctttt gccagatgtg ttagggaatc
    16201 agacttcagc tcaaacggat tcttattcaa ccggtttatt ggaacatccc caatatactc
    16261 gcccggcaga atataaaggg atgaaagtac cagaagtatt gacaaatgga aatcataaat
    16321 tgattgaaga atggcaatta aaagaatcac taagaaggac ctatcaacgt cggcctgatc
    16381 ttttggaaac acttgaatgg acacctcaaa tgacgaaatt tttagaagaa atcaagaaag
    16441 aagaacaaga aaaaacagca gatgaagcca agtgactttt tctaaaatct tctttacagt
    16501 aggttgataa tatgttagta ttttatggtg agtgcaaaag cacttaacta ttacgatatt
    16561 ccgctgtgag aaaagctcat aagaatattt ggaataagga gaagaaaacc atgaatccat
    16621 taatcgaaga attaacaaaa gaacaattac gttctgacat cccagcgttc cgccctggtg
    16681 acactgtgcg tgttcacgca aaagttgttg aaggtactcg tgaacgtatc cagttatttg
    16741 aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga aacttataca gtacgtaaag
    16801 tttctaacgg agtgggcgtt gaacgtacat tcccattgca tacaccacgt gttgcaaaaa
    16861 tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt gtactaccta cgtgcattac
    16921 acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc attgttatca gactgagaaa
    16981 gaacccttgg tattcaaggg ttcttttttc ttagcatgta atttgaacac cctgatccag
    17041 ttttttcttt aattattggt aggggagaat aatggattag aataaatcta cctataagta
    17101 gtagtaaaaa aaagagcact atgataatat ctttcttaag agtaaccgct ttaataaaaa
    17161 atccagaata ttctaacata aatggagtat tcttagaatc aaaaggagaa agaagatggt
    17221 ttatacttat acggagcaag aaaagatgaa agttttagac acattcatga atgatggaaa
    17281 agtaaaacaa gtaccaagtt caacaaaaaa gaaatacatc ctattgaaag aagtcttgaa
    17341 acgctttgac catggagtga cttatacaga aacagaggtt aattcgatac tgctgaacgt
    17401 attttctagt ggcgattatg tcgaacaaag acgttatcta atcacatttg gctttttcaa
    17461 aagaagttca gacggtcggg catatcagat gatgggaatt gaaaattaga agttttaatt
    17521 tatactcatt gaaaaaagtt caagtatgcg atattggttc tacaccgcat acttgaactt
    17581 tttttgtcat ctgtatctat aatacaaagc caagaacgtt attcaaaata tggatagcta
    17641 tgataaaagc taatgagcga ttcgcagtta aataagcaat cgtcagtaaa atcgttccag
    17701 gagcgtacat gaaaaaatca attggatgcg ttacatgaat aaagataatt ctcttttatt
    17761 tgtacgaata aataccaaaa cgtacctcct aaaattagat tttggacaga tgccctttaa
    17821 aaagataaca agatttcgtt cttgaaacaa tatatatgct tttattccaa tgaggttgtg
    17881 aaaacagtat tttgtcctga gcgttagaaa attgaaaatg gggaatagca actcgtactt
    17941 taccattttt taagtgaata caagatcagc taattgaaca ccgtttattc gtgatgaggg
    18001 acacaacact gcagtcgaca catttctctt ttatgttcaa agataacaaa tatttaaaac
    18061 aa
//
LOCUS       DACDVT010000049        17752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDVT010000049 DACDVT010000000
VERSION     DACDVT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.049"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="IWL52_001761"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="IWL52_001761"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9778307.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="IWL52_001762"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="IWL52_001762"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9778308.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="IWL52_001763"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="IWL52_001763"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9778309.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3413)
                     /gene="tsaB"
                     /locus_tag="IWL52_001764"
     CDS             complement(2589..3413)
                     /gene="tsaB"
                     /locus_tag="IWL52_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9778310.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3502..4482)
                     /locus_tag="IWL52_001765"
     CDS             complement(3502..4482)
                     /locus_tag="IWL52_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9778311.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4506..5990)
                     /gene="galT"
                     /locus_tag="IWL52_001766"
     CDS             complement(4506..5990)
                     /gene="galT"
                     /locus_tag="IWL52_001766"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9778312.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6002..6991)
                     /gene="galE"
                     /locus_tag="IWL52_001767"
     CDS             complement(6002..6991)
                     /gene="galE"
                     /locus_tag="IWL52_001767"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9778313.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7239..7406
                     /locus_tag="IWL52_001768"
     CDS             7239..7406
                     /locus_tag="IWL52_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9778314.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7468..9279)
                     /locus_tag="IWL52_001769"
     CDS             complement(7468..9279)
                     /locus_tag="IWL52_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9778315.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9276..9641)
                     /locus_tag="IWL52_001770"
     CDS             complement(9276..9641)
                     /locus_tag="IWL52_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9778316.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9804..10199)
                     /locus_tag="IWL52_001771"
     CDS             complement(9804..10199)
                     /locus_tag="IWL52_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9778317.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10217..11179)
                     /locus_tag="IWL52_001772"
     CDS             complement(10217..11179)
                     /locus_tag="IWL52_001772"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9778318.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11179..11391)
                     /locus_tag="IWL52_001773"
     CDS             complement(11179..11391)
                     /locus_tag="IWL52_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9778319.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11412..12110)
                     /locus_tag="IWL52_001774"
     CDS             complement(11412..12110)
                     /locus_tag="IWL52_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9778320.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12130..12672)
                     /locus_tag="IWL52_001775"
     CDS             complement(12130..12672)
                     /locus_tag="IWL52_001775"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9778321.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12804..13808)
                     /locus_tag="IWL52_001776"
     CDS             complement(12804..13808)
                     /locus_tag="IWL52_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9778322.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13805..14794)
                     /locus_tag="IWL52_001777"
     CDS             complement(13805..14794)
                     /locus_tag="IWL52_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9778323.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14791..15597)
                     /locus_tag="IWL52_001778"
     CDS             complement(14791..15597)
                     /locus_tag="IWL52_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778324.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15763..16719
                     /locus_tag="IWL52_001779"
     CDS             15763..16719
                     /locus_tag="IWL52_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9778325.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16796..17314)
                     /locus_tag="IWL52_001780"
     CDS             complement(16796..17314)
                     /locus_tag="IWL52_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9778326.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="IWL52_001781"
     CDS             complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="IWL52_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9778327.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         5254 a         3866 c         3067 g         5565 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagttgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     3481 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     3541 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     3601 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     3661 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     3721 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     3781 aggaacatgc ggattcgttt ttaaatactg cttcactgct tgatatcctg actcgacaga
     3841 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     3901 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     3961 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4021 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4081 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4141 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     4201 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     4261 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     4321 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     4381 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     4441 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     4501 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     4561 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     4621 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     4681 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     4741 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     4801 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     4861 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     4921 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     4981 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5041 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5101 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     5161 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     5221 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     5281 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     5341 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     5401 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     5461 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     5521 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     5581 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     5641 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     5701 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     5761 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     5821 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     5881 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     5941 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6001 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6061 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6121 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     6181 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     6241 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     6301 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     6361 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     6421 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     6481 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     6541 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     6601 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     6661 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     6721 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     6781 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     6841 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     6901 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     6961 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7021 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7081 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     7141 aggatttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     7201 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     7261 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     7321 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     7381 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     7441 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     7501 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     7561 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     7621 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     7681 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     7741 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     7801 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     7861 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     7921 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     7981 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8041 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     8101 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     8161 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     8221 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     8281 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     8341 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     8401 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     8461 ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg atggatgata
     8521 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     8581 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     8641 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     8701 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     8761 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     8821 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     8881 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     8941 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9001 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9061 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
     9121 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
     9181 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
     9241 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
     9301 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
     9361 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
     9421 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
     9481 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
     9541 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
     9601 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
     9661 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
     9721 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
     9781 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
     9841 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
     9901 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
     9961 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10021 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10081 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    10141 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    10201 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    10261 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    10321 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    10381 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    10441 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    10501 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    10561 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    10621 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    10681 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    10741 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    10801 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    10861 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    10921 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    10981 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11041 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    11101 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    11161 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    11221 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    11281 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    11341 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    11401 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    11461 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    11521 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
    11581 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    11641 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    11701 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    11761 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    11821 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    11881 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    11941 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12001 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12061 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    12121 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    12181 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    12241 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    12301 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    12361 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    12421 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    12481 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    12541 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    12601 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    12661 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    12721 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    12781 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    12841 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    12901 cttagtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    12961 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13021 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
    13081 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    13141 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    13201 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    13261 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    13321 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    13381 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    13441 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    13501 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    13561 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    13621 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    13681 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    13741 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    13801 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    13861 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    13921 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    13981 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14041 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    14101 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    14161 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    14221 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    14281 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    14341 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    14401 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    14461 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    14521 agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa
    14581 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    14641 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gcttttccca
    14701 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    14761 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    14821 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    14881 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    14941 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15001 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15061 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    15121 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    15181 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    15241 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    15301 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    15361 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    15421 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    15481 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    15541 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    15601 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    15661 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    15721 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    15781 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    15841 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    15901 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    15961 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16021 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16081 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    16141 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    16201 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    16261 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    16321 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    16381 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    16441 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    16501 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    16561 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    16621 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    16681 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    16741 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    16801 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    16861 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    16921 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    16981 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17041 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    17101 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    17161 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtgtgttcgc
    17221 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    17281 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    17341 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    17401 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    17461 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
    17521 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    17581 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    17641 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    17701 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt at
//
LOCUS       DACDVT010000050        17752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDVT010000050 DACDVT010000000
VERSION     DACDVT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.050"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            41..853
                     /locus_tag="IWL52_001782"
     CDS             41..853
                     /locus_tag="IWL52_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9778328.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            850..1650
                     /locus_tag="IWL52_001783"
     CDS             850..1650
                     /locus_tag="IWL52_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9778329.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSTVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1811..3079
                     /gene="rseP"
                     /locus_tag="IWL52_001784"
     CDS             1811..3079
                     /gene="rseP"
                     /locus_tag="IWL52_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9778330.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3147..4856
                     /locus_tag="IWL52_001785"
     CDS             3147..4856
                     /locus_tag="IWL52_001785"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9778331.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5068..9420
                     /locus_tag="IWL52_001786"
     CDS             5068..9420
                     /locus_tag="IWL52_001786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9778332.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9564..10037
                     /gene="rimP"
                     /locus_tag="IWL52_001787"
     CDS             9564..10037
                     /gene="rimP"
                     /locus_tag="IWL52_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9778333.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10061..11236
                     /gene="nusA"
                     /locus_tag="IWL52_001788"
     CDS             10061..11236
                     /gene="nusA"
                     /locus_tag="IWL52_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9778334.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11258..11551
                     /locus_tag="IWL52_001789"
     CDS             11258..11551
                     /locus_tag="IWL52_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9778335.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11548..11859
                     /locus_tag="IWL52_001790"
     CDS             11548..11859
                     /locus_tag="IWL52_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9778336.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11872..14178
                     /gene="infB"
                     /locus_tag="IWL52_001791"
     CDS             11872..14178
                     /gene="infB"
                     /locus_tag="IWL52_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9778337.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14205..14552
                     /gene="rbfA"
                     /locus_tag="IWL52_001792"
     CDS             14205..14552
                     /gene="rbfA"
                     /locus_tag="IWL52_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9778338.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14660..14881)
                     /locus_tag="IWL52_001793"
     CDS             complement(14660..14881)
                     /locus_tag="IWL52_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9778339.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15329..17692
                     /locus_tag="IWL52_001794"
     CDS             15329..17692
                     /locus_tag="IWL52_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9778340.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6053 a         3148 c         3911 g         4640 t
ORIGIN      
        1 ttaagatagt gggaatcttt taagtatagg aggaaaaaga atgttacgtt tttttccgca
       61 aaaaaacaaa tatattcaag aaaaaagcga atttcaattt aataccgatg gaccaattcc
      121 tcagcacatt gccatcatca tggacggcaa tggtcgttgg gcgcaaaacc gtcgtttgcc
      181 acgagttgct ggccataaag aaggcatgga aacagtaaag aaagtcacaa aaaaagcatc
      241 aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg actgaaaact ggaagcgacc
      301 aaaagatgaa gtaagttttc tcatgcaact acctgtcgac ttctttgata cgttcgtacc
      361 agaattgatt aaagaaaatg tcaaagttca tgtcatgggt tatgaaaacg tattgccaga
      421 acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca aaaaacaata ctggaatggt
      481 attgaatttt gctttgaatt atggaagtag agcagagatc gtgactgctg tccaagaaat
      541 tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa atcgatgatg aattgattgc
      601 taagcattta atgacaggtt tcttgcctaa agagttacaa gatcctgagc tgatgatccg
      661 tacaagtgga gaagagcgaa ttagtaattt tctgttatgg cagatcgcat atagtgagct
      721 gtattttaca aaagcgcttt ggcctgattt tgatggtgcg catcttgaag aagcaatcgc
      781 ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa aaaacagaag atgagaaagg
      841 ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg cgctagtctt gtttattccg
      901 attatatgga tcggaggcat agcggttgag cttacagcag caatcttagc ggtagtagga
      961 gtttacgagc tgttccggat gaaaggattg acactcttaa gttttgaagg agttttatcc
     1021 gccgttggtg cagtgttact tgttttgcca aaagaaagat ggtttttctt tcttccagag
     1081 aatgtaagta catttatgtt attttatctt atagttatga ttcttttagg aacatcagtt
     1141 atttctaaaa atacgtatac gattgatgaa gcaggttttc ctgtgcttgt cagcttgtat
     1201 gtgggaatag gtttccagaa ttttgtcaat gcacgaacag aaagcttggc tgttttgata
     1261 tttggtctat ttattgtttg ggcaactgat atcggagcat atatggttgg tcgacgttat
     1321 ggtcagcata agttaagtcc tgatatttcg cccaacaaaa cgattgaagg tgcgctcggt
     1381 ggtatcggta gtactgttgt agtcgcattt ctttatttat tgctataccc agcaaaagaa
     1441 ctattcaatc atggtatgtt tgtcatgctt ctgatgacga ttattttttc agcagtcgga
     1501 caatttggtg atttggtcga atctgcaatc aaacgtcatt atgatgtgaa agactcggga
     1561 aatatcttac caggccatgg tggaatctta gaccgtttcg acagtttgct gtttgtattt
     1621 cctatcatgc atttatttgg cattatttag taagtatacc tgcgtttctt tgtgaatttc
     1681 ataaaagacg caggttttta tttttcatta tattgatact attggtgtct ttggtatact
     1741 atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa cacaaaaaac aagtaaagga
     1801 gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt tttggtatat tagtgattgt
     1861 tcatgagttt ggtcatttct tctttgcgaa acgatcagga atcctcgtcc gcgaatttgc
     1921 tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa gacgggacaa catatacgct
     1981 tcgcctatta cctattggcg gttatgttcg aatggcaggc aatggggatg atgaaacaga
     2041 aatggcacca ggaatgcctc tttctctttt actaaattct gacggcatcg tagagaagat
     2101 aaatttaagt aagaaaatcc aattgacaaa tgctatcccg atggaattga gccgttatga
     2161 tttagaagat gagctgacta tcactggtta tgtgaatgga gacgaaacag aagtagtaac
     2221 ctaccctgtt gatcacgatg cgacgattat tgaaaacgac ggaacagaaa tcagaattgc
     2281 gccaaaagac gtacagttcc aatcggctaa attgtggcag cgtatgctga ccaactttgc
     2341 tggacccatg aataacttta ttttggcaat cgtgttattc atcattctgg catttatgca
     2401 aggtggtgtt caagtgacga acactaatcg cgtaggagag atcatgccta atggagctgc
     2461 ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt gtggacggaa aagaaatcca
     2521 ttcatggaat gatctgacaa cagtcattac gaaaaatcca ggtaaaacat tagactttaa
     2581 gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca ccgaaatctg tagagtcaaa
     2641 tggcgaaaaa gttggccaac ttggtataaa agccccaatg aatacaggtt ttatggataa
     2701 gatcattgga gggacgcgtc aagctttcag cggttcattg gaaattttca aagccttagg
     2761 atcactattt acaggtttca gtttagataa attaggcgga cctgtcatga tgtaccaatt
     2821 gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt agtttgatgg cgcttctttc
     2881 aatgaatctc ggaatcgtca atctgcttcc gattcctgcc ttagatggcg ggaaattagt
     2941 cttgaatatt tttgaaggga ttcgcgggaa accattaagt caagaaaaag aaggaatatt
     3001 gacattagct ggctttggat ttttaatgtt gttgatggtg ctagttacat ggaacgatat
     3061 tcaacgattc tttttctaga aaaagttgac aaatagggta taataacaaa cgataactat
     3121 tttgaggaaa taaaggagta tatagaatga gacaatcaaa aatgttgatt ccaacattac
     3181 gcgaagttcc aaatgatgca gaagtattaa gccatcaaat cttgctaaga gctggatata
     3241 tccgccaagt atcagcgggt atttattcat atctgccatt agcaaatcgt gtattggaaa
     3301 aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg agcggtagaa atgctgatgc
     3361 ctgccatctt gccagcagaa ctttgggaag aatccgggcg ttacgaaaca tatggaccaa
     3421 acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat ccttggacca actcacgaag
     3481 aaacttttac tgatttgatg cgtaatgaaa tcaattcata taagaaatta cctttaaatc
     3541 tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc tcgcttcggt ttgcttcgcg
     3601 gaagagagtt tatcatgaaa gatgcatatt ctttccatgc aacggaagaa agtttggacg
     3661 agacatataa agattacgaa aaagcctata ccgaagtatt caagcgttgt ggcttagaat
     3721 ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa agactcaaag gaattcatgg
     3781 cgatctcaga aatcggggag gatacgatct gttattcgac tgaaagtgat tatgctgcaa
     3841 acttggagat ggctacaagc tactatgtat caaaaaaatc gcatgaaaca cagcttgaat
     3901 tggagaaagt agctacacct aacgtaaaat caattgacga agtagcagat ttcttttctg
     3961 ttgaacctca aaaaatcatc aagtctgtat tgttcattgc ggatgaacaa cctgtccttg
     4021 ttttggtacg tggagaccat gaagtcaatg acgtaaaact gaaaaacttt ttatctgctg
     4081 actttttaga agaagcaaca gaagaagaag cacaaaaata tctaggtgct gagtttggtt
     4141 ctgtcggacc agtcggtgtt tcagatgacg taaaagtata tgcagaccgt tatgtccaag
     4201 atttagcgaa tgccattact ggagcgaacg aaacaggatt ccactataaa aatgtgaatc
     4261 caaaacgcga tttcactgtg ctttcttatg aagatcttcg ttttgtacaa gaaggagatc
     4321 cttcaccaga caacaacggt gttcttgcct ttactcgagg aatcgaaatc ggacatatat
     4381 tcaaaatcgg cactcgttac agtgaatcta tgggagcaac tgtactagac gaaaatggac
     4441 gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt aagtcgttta ttatcagcaa
     4501 tcgtagaaca acattcagat gaacaaggaa tcaattggcc aaaaggaatt gcgccatttg
     4561 atctgcatat cgtccaaatg aatctaaaag acgaatacca aacaagcctg acgaatgaac
     4621 tagaagaagc gatgacaaaa gctggctatg aagtactggt tgatgacaga aatgaacgcg
     4681 caggagtgaa attcgcggat tctgatttga ttggctgtcc aatcagaatc actgtcggta
     4741 aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa aactggcgaa atggttgaag
     4801 tacgtaaaga agaattggcg aatactttac caatcctctt gaaccaaggc gaataataag
     4861 cgttaaaaca cagaaaataa aaatagctga atcagctgtt tttgacaatc tgatgattcg
     4921 cttttaaaat tggaactgcg atttgaatcg cagttccaat tttttaatgt aaaactcaga
     4981 aaaaacgagt atactaaaag gtactgaaaa agaagaggat aagatgcgat caattgcatt
     5041 gtttctcatc agaaagggcg tatatctttg tcagaaaaac gagatttatt tgaaaaactg
     5101 ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc cactgcttac tgctggtgaa
     5161 atggatagtg tagtggtcca tcgtaaatcg cgtttatggg aatttacgct acatttttcc
     5221 aaaattttgc ctattatgct atatcggtca ttgactcagc atctgactat cgcttttaaa
     5281 gatattgcac aagtcaaact gaatatctta gcagatgacc agacattcga tgaacagctc
     5341 ttgcaagatt actgggccca agctttggaa aatcagcaat gcgatacacc actagcacag
     5401 caagtattga aaacgcaggt acctgtgata aaagatcgta aagtcatctt gccaatcgac
     5461 agtacaggag ctatttctta tttgaagcaa caatacttgc cattagtaga agagttgttc
     5521 gtcagttacg gattcccaaa atttcgtatc gaaccagaag tggatgagca gcaagcggaa
     5581 cgtgtattga aactttttga agaacgcaaa caagaacagg cagaagcctt tatgaagcaa
     5641 gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg aaagaaaaga gcaatcgcca
     5701 gctcttgaag gacccatcca gctaggacga aatattccag ccgatgaacc aacgacacca
     5761 atgatcagta tcgttgaaga agaacgaaga gtcacacttg aggggtacgt attcgacaaa
     5821 gaagtcagag aactacgttc aaaacgcaag atcttgactt tgaaaatcac agattatact
     5881 tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag atgaacaagt atttgatgcg
     5941 atcagtgtcg gtagctggtt aaaggtacga ggaagtattc aagaagatac gtttgtccgt
     6001 gatttggtca tgaatgcgca agatatcata gaagtcaaac atacacctcg caaagattat
     6061 gctcctgaag gggaaaaacg agtagagctt catgttcata gcaatatgag tacgatggat
     6121 gcaacaaaca gcatctctga tcttgtggca caagcaggaa aatggggaca ccgagctatc
     6181 gccattacag accatggtgg tgcgcaagcc tttccggaag cacatagtgc tggaaaaaaa
     6241 gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg tagatgatgg tgtcccaatt
     6301 gcttataatg atgcacatga agcattgagt gaagccacat acgtcgtatt tgacgtggaa
     6361 acgacaggat tgtctgcagt atatgacacg atcatcgagc tagccgcagt caaaatgtat
     6421 aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc caggacatcc attgtcacga
     6481 acaacggtag atctgactgg tatcacggat gaaatggttc gagggtcaaa gtcggaagaa
     6541 gaagtattgc ggatgttcct tgaattttca aaagatacaa ttttggtagc ccacaacgcg
     6601 gcttttgata tggggttctt gaatactagc tacgcaaggt atggtattcc tgaagcggcc
     6661 aatccagtca ttgatacgtt ggaattagct agatatctgt atcctcaatt caaacgtttc
     6721 ggattaggtg tgttatctaa aaagtttggt gttagcttgg aacagcatca ccgagcaatc
     6781 tatgatgctg aagcaactgg tcacttagca tggatctttg tgaaagaggc aatggacaat
     6841 cataacatgc tttatcatga tcagctaaat gagcatatcg gagaaggaga ttcttacaaa
     6901 agggctcgac ctttccatgt aacgatttta gcaaaaaatc aggcaggact gaaagatttg
     6961 ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac gcgtcccacg aataccacgc
     7021 tcccaattga aaaagatgcg tgaaaatcta ttgatcggct cagcttgtga taagggagaa
     7081 atattcgaag ccatgatgca aaaaggggta gaagaggcaa gaaaccgagc aaaattctat
     7141 gattatattg aagttatgcc aaaagcggtc tatgcgcctt tgatcgagca agaattggta
     7201 aaaaatgaac atgatttgga agaaattatc caaaatttgg tagaaatcgg aaaaagtttg
     7261 gataaaatcg ttgttgcaac aggtaatgta cattatctga acgaagaaga tgccatttac
     7321 cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt tgaatcgtca tagtttgccg
     7381 gatgtccatt tccgaacaac cgatgagatg ctaactgcat ttcacttttt aggggaagaa
     7441 acagcgaaag agatcgtagt agaaaatacg aataaaatcg cagatatctg cgaagaagtg
     7501 attccagtca aagatgaact ttatacaccg aagattccag gttcagaaga tgaaatatca
     7561 gaattgagtt acacaaaagc aaaacaaatg tacggagatc cgttacctga gatcattcaa
     7621 aaacgattag aaaaagagtt gaattctatt aatggtaatg gcttctcggt tatttatctg
     7681 atcgcccaaa aactagtgca taaaagtaat gaagatggct atctagtagg ttcacgtggc
     7741 tctgttggct ctagctttgt tgcgacgatg acaggaatca ctgaagttaa tccattagcc
     7801 cctcattatt attgtccaga gtgccaatat tccgagtttt ttgaagatgg tacgtatggt
     7861 tctggatttg acatgccaga aaaacaatgt ccaaaatgtg gtgcacgctt aaacaaagac
     7921 ggtcatgata ttccatttga gacattttta ggattccatg gagacaaagt gcccgatatt
     7981 gatttgaact tctcaggtga ttatcaagca gaagcccata actatacgaa agtattgttt
     8041 ggcgaagact atgtttaccg tgccggaacg attggtacag tcgccgacaa aacagcatat
     8101 ggctatgtaa aaggatacga aagggacaat aatctgcagt ttcgaagtgc agaagtcgac
     8161 agattagcaa aaggcgctac tggagtcaaa cggacaaccg gccagcatcc aggggggatc
     8221 atcgttattc ccgattatat ggatgtctat gattttacgc cgatccaata tccagcagac
     8281 gatcaaaatt cagaatggaa aacgactcat tttgatttcc actcgattca cgataatgta
     8341 ttgaaattag atattctagg gcatgatgac ccaactgtta ttcggatgct tcaagattta
     8401 tcagggatcg acccacaaac gatccctacg gatgacccgg aagtgatgcg gatatttgcc
     8461 ggtcctgaag tacttggagt aagtcaggaa caaatttatt ccaaaacagg gacattaggt
     8521 attccagaat tcggcactcg ttttgttcga gggatgttag aagaaacgca tcctacaact
     8581 tttgcagaac tactgcaaat ttccggacta tctcatggta cagacgtatg gttaggaaat
     8641 gcggaagaat tgattcgacg aggagatgcg acactggcgg aagtaatcgg ctgtcgtgat
     8701 gatattatgg tttatttgat ccatgctgga ttagatagcg ggatggcatt taaaatcatg
     8761 gaaactgttc gtaaaggaca atggaacaag atccctgatg aattgcgaga aacctatcta
     8821 tcggcgatga aagaaaataa tgtgcccgac tggtatatcg attcttgttc aaaaatcaaa
     8881 tatatgtttc cgaaagccca tgctgcagct tacgtattga tggctcttcg tgtcgcttat
     8941 ttcaaggttt atttccctat tttgtattac tgtgcatact tttctgtacg tgcagacgac
     9001 tttgatctag tctccatgtg caaaggaaaa gatgctgtga aacaagcaat gaaagaaatc
     9061 acggacaagg gattagatgc ttccgtaaaa gaaaagaatc aactgacagt gctggagtta
     9121 gccaatgaaa tgctggaacg aggattcaaa tttggaatga ttgatttgta taaatcggat
     9181 gccgttaact ttgttattga aggtgatacg ttgatcgcac catttagagc tgtcccaagt
     9241 ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa aagacggtcc atttttatcc
     9301 aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat tgatcgaata tatgaacgac
     9361 aatggcgtat tgaaagatct accagatgaa aatcaattat cgttattcga tatgctataa
     9421 gcatctgttt tcctgtctgc ttgtaatttt aagcagagta tggtatagtt cttataggta
     9481 atgatactaa ctgtgagtga gcggattttt cgctcactct ttttaatgga attacgcact
     9541 taatttttaa ggaggcgaaa cttttgagta gcgtcgttga aacagtcaca gaaatggtga
     9601 caccgatttt agatgaacaa aattttgaac ttgtcgaagt cgaatttgta aaagaaggaa
     9661 aaagctggtt cttacgagta tttattgaca aagaaggcgg tatcgatatc gaagaatgcg
     9721 cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc agatccagat ccgattcctc
     9781 aagcttattt cctggaggta tcctccccgg gagcagagcg gccattgaaa aaagaaagcg
     9841 actatgaaaa ggcacttggt gattatgtcc atgtctcctt ataccaacca gtagatggcg
     9901 aaaaacaata tgaaggattt cttcaatcat ttgatgctga acagcttaca ttgaaaatac
     9961 gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa aaatattgca aaagctcgct
    10021 tagctatcca attttaatca cggagaggaa tcagaaaaaa atgagtaaag aaatgttaaa
    10081 tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa gaaatcgtga tcgatgcttt
    10141 agaagcagca ttggtttctg catacaaacg tcactatggc caagcacaaa acgtcgaagt
    10201 ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg gtaaaagaag taacggaaga
    10261 agtgatggat tcacagttgg aagtctcatt gaaagatgca cttttaatca atccagcata
    10321 tgagatcggt gatacgatcc gttttgaagt aacaccaaaa gattttggac ggatcgctgc
    10381 gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa gcagaaagaa cgattattta
    10441 taacgaattc agtgcttatg aaaaagacat catgcaagga atcgtcgaac gccaggataa
    10501 acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt ctttctaaac aagatcaaat
    10561 gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt tatgtttccc gagtagaaaa
    10621 tacgtctaaa ggtccgcaag tatttgttag ccgcagccat ccagacctat tgcgtcgctt
    10681 atttgaacaa gaagtaccag aagtctatga tggaatcgtt gaaattgtca gcatcgcccg
    10741 tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat gatccaaata tcgatgcagt
    10801 cggtacatgt gtcgggccaa agggacaacg agttcaagca atcgtcaatg aattaaaagg
    10861 cgaaaacatg gatatcgtcg aatggaatga agatccagct gtattcatcg caaatgcatt
    10921 gaatcctgca caagtcatgg atgtgatctt cgatgagaca aatccaaagg cctgcacagt
    10981 tgtcgttcca gactaccaat tatcgctagc aatcggtaaa cgcggacaaa atgcacgttt
    11041 agctgctaaa ttgactaacc ataaaatcga tatcaaatca gaatctgaca tgacggaatt
    11101 ttatgaaaat caagaacaac aaaaagaaac agaagaactg catgatgaag caatcgttca
    11161 gtcagattta acagacgatg aatatgaaac aattgcattc aacaatgaaa cggtcgaaga
    11221 aaaaccagaa gcttaacttt agtaaggagg caaagagatg aaacaaagga aaattccttt
    11281 acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa gagatggtcc gaatcacccg
    11341 ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa atgcctggac gaggagcgta
    11401 tgtctcactt gagccggaag aagtacaaca agcttgggat aaaaaaattc tcgatcgagt
    11461 actcgaaaca acattaacag atgagtttta tcaagaactt cttgactatg tcactcacca
    11521 aaaagcacgt aaagagttat ttggaaaatg aatacagtta atcggcaaaa agccatgaat
    11581 ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg gtgaagaact gaccattgct
    11641 gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa ccgatgcaag tgagaataca
    11701 agaaaaaaga taaaagataa gagttcatat tatgaagttc cttgctttga gctattttct
    11761 gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg tattcggaat aatggatgcc
    11821 ggatttgcga agaagaccaa ggagctaatt gaaggttagg aaggtgattg catgggcaac
    11881 aaaagaatct atgaattagc aaaagaactc aaaaaatcta gtaaagacgt cgtggaaaaa
    11941 gcacaacaac ttggcatcga tgtgaaaaac catatgggtg cgattactag cagtgatgag
    12001 aagaaattgc aacaagcgtt caaaaaccca agcacgaaac aacaagctgc acaacaagca
    12061 agcagaaaac cagccaatca acagccgaac gatcaacgta aaaaagaaac aacgaacaat
    12121 caacaaaaaa ataaaaataa ccgcagttat caagatcgcg gtcaagggag cggtcaagtg
    12181 aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg gaaataccag cagccaaaat
    12241 cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta acaataacaa caaaccaaac
    12301 aataacaatc gcggcaaatt taataataat aaccgtaatc gttataataa aaaaggtaaa
    12361 aaaggcaaac agcaacaatc aactaaacca gctgtaccac cacgcaaatt ccgtgaattg
    12421 ccagatgtat tagagtatac tgaaggtatg aacgtagcag atattgcgaa aaaaattcat
    12481 cgtgagccag cagaaatcat taaaaaatta tttatgcttg gtgtcatggt caatcaaaac
    12541 caagcactgg ataaagatac gattgaactg ttagcgacgg actatggcat ggaaccacaa
    12601 gaaaaagtcc aagttgacat tgctgatatc gataaattct tcgaggcaga agaagtcgat
    12661 ccagataaat tagtctcacg tccgccagta gtaacgatta tgggacacgt agaccacggg
    12721 aaaacaacct tattggatac attgcgccat tcacgtgtaa caagcggcga agccggcgga
    12781 atcacacagc atatcggtgc ctaccagatc gatattgatg gtaaaccaat cactttcttg
    12841 gatacaccag gacatgcggc ctttacaagt atgcgtgcac gtggagcaag tatcacagat
    12901 atcacgatct tagttgttgc tgcagatgat ggcgtcatgc cacaaacagt tgaagcaatc
    12961 aaccatgcca aagctgcagg cgttccaatc atcgtcgctg taaataaaat tgataaacca
    13021 ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt atgaattaat tcctgaagca
    13081 tggggtggag aaacgatttt tgttgagatc tcagcgaaat tcggccaaaa catcgaagaa
    13141 ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt taaaagcaga tccaacacaa
    13201 cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag gtaaaggtcc tgtgtcaacc
    13261 ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa tcgttgtcgg taatacttac
    13321 ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg ataaagaagc aggacctgca
    13381 acgccagtag aaatcactgg cttgaatgat gtacctcaag caggtgaccg tttcgtagtc
    13441 ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac gcgcaaaacg tgcattattg
    13501 gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact tatttgaaag cctgaaagaa
    13561 ggcgaattga aagaagttaa cgtaatcatc aaagcagatg tacaaggttc agcagaagca
    13621 ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc gtgtaaaaat cgtccattca
    13681 gctgtcggag caatcaatga aagtgatgta accttagcag cagccagcaa cgctattatc
    13741 atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc aagctgatac tgaagaagtc
    13801 gatattcgtc ttcaccgtat catctataaa gccatcgaag aaatcgaaac tgcgatgaaa
    13861 gggatgctag accctgaatt tgaagaaaaa atcaccggtc aaatgatcgt tcgtgaaacc
    13921 ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg taacagaagg atatattcgt
    13981 agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa tctatgaagg ccaacttgca
    14041 agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa tgggttatga atgtggtgcg
    14101 atgattgaaa aattcaatga catcaaagta gatgacgtaa ttgaaggatt tatcatggaa
    14161 gaagtcaaaa atgactaaaa actgataaaa ggaggccaat cattatggct aactaccgtg
    14221 atcgccgagt cggtcaagaa atcttaaaag aagtcaatga tattttgcgc aaaaaggtcc
    14281 gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa agttacgggg gacttacagc
    14341 aagcaacgat ctattacagc attttatctg acttagcatc agataagaaa aaagcacaag
    14401 atggattgaa taaagcatca ggattgattc gtcgagagtt agggcacaat ctaagtattt
    14461 ataagacacc tgaattgttc tttgaacttg atgaatcagt tgtctatggg aaccatatcg
    14521 atgaattgct tcgcgacttg aacaaagact aatcaaaaaa tcccgcaaat cctgtgaaat
    14581 caggggttgc gggatttttt tgttttcatt aagacgtatt acctaagagt tccatgactt
    14641 tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag aaaagtaaaa aattcgactt
    14701 tcttttttct atagtatata aatttttgca tatagataaa gatcaagata ccgataacgg
    14761 aagctagatt gtaatctatt ttagtgacag tcaggtatag ccaagtaact agaagatcag
    14821 tggaacctgc accataaaag tttgagtttg tcggtccgtt gacagttaaa agaaaaatca
    14881 cgttgaagtt attgatattt ccgattacgc cgcttcgatt tggtcacgat ccaaattttg
    14941 gataatccct gtagaaatta gcatagtagc tgggatacca attcacatat tgacaaagat
    15001 aacagagact tttacataaa acggatcaat caggaaagga attgctcgat ccagccccag
    15061 ttttccaaca atgtgttaac tggcctagcg tcattcagca aattatgcat gatgaggagc
    15121 gaaatgaact gcggaacagc cattgtgata acgacaagtt ttttggatca atgtaaaaaa
    15181 cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt tcatttggct agatataaaa
    15241 tccaaacaaa tgaacagaag tgcttattag gtttcaattg ggagtatctg cgctagaata
    15301 agagtaataa tgaaaaggag tgttatgtat ggattattct agtaaagaat actttgataa
    15361 aatgacggct tggtggcgtg ctgctaatta tctttctgtt gggcaacttt atcttaaaga
    15421 taacccctta ctgagacgta cactgaaacc agaagatgtc aaaaaacatc caatcggtca
    15481 ttggggaacg attcctggcc aaaattttat ttatgtccat ttgaaccgag tcatcaataa
    15541 atatgatttg aacatgtttt atatcgaagg gccaggacac ggcggacaag tcatggtatc
    15601 gaatgcttat ttagatggaa gttacacaga gatctatcca gaagtaacgg aagatgagac
    15661 aggaatgcaa aaattattca agcgattttc atttcctgga ggcattgctt ctcatgcagc
    15721 accagaaaca ccaggttcta tccatgaagg cggagagctt ggatactctc tttcacatgc
    15781 agtcggagct gtattagaca atcctgaagt aatctctgct gtcgtgattg gtgatggtga
    15841 agcagaaacc ggtcctttag ctggaagctg gttctcaaat gtctttatca atccagtgat
    15901 agatggagct gttttaccga tccttcattt aaatggtgca aaaatagcca acccaacgat
    15961 cttggcacgt aaatcagatg gagaattagc aaattacttt aacggacttg gctgggagcc
    16021 tttctttatt gaaggaaatg atccagaaaa attaaatcct gttatggctg aaaaaatgga
    16081 tcaagcaatc gagaaaatca aatccatcca aaaagaagct cgcctgaaga cagcaacaga
    16141 tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca ccaaaaggat ggaccggtcc
    16201 aaaagaatgg gacggggaac caattgaagg aaccttccga gcacaccaag taccgattcc
    16261 tgtagatcaa gaacatatgg atcatgcaga tgctttactc cgttggctga aatcctatga
    16321 accagaaaaa ttatttgatg cacaaggacg tattttagaa gaaatcagag aaattgcgcc
    16381 tactggtgac caaagaatgg caaaaaatcc tatcaccaat ggtggaatcg atccaagacc
    16441 gcttattatg ccagattgga aaaaatatac tttgcaattt gaaaaaccag gctccgttaa
    16501 agcagaagat atgaccgaac ttggtaaatt cgttcgcgag atcattgaga aaaatccaga
    16561 aaatttccgt atatttggac cagatgaaac aaaatcaaac cgattgaatc aagttttcaa
    16621 aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa aatgatgaat ggctttcgcc
    16681 atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa gatgaagggt tcttagaagg
    16741 gtacgttctg actggacggc atggtttttt tgcaagttac gaatcattct tgcgagtggt
    16801 tgactctatg ttgactcagc acttcaaatg gatacggaaa tcacatgacc tttcttggcg
    16861 aaaagattat ccttcgctga atctcattgc aagctcaaca gtcttccagc aagatcacaa
    16921 tggatatagc caccaagatc caggaatatt gacacatttg gcagaaaaga aggcggagtt
    16981 catccgtgaa tatctgcctg cggatgcaaa tacattgctc gcagtcatgg ataaagcatt
    17041 ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa catccgcgtg ctcaatttta
    17101 ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg aaaatcattg attgggcttc
    17161 gacagcaaaa gaggaggaac ctgaattggt cattgctgct gcaggaacgg aatctaactt
    17221 agaagcatta gcagctgtaa ctttgctgct ggaagaattc ccaaaactaa aaattcggtt
    17281 catcaatgtc gtagatttat tgaaattacg tcatccaagt caagatccgc gtgggttgag
    17341 cgatgaagaa tttgatcaat attttacaaa agacaaaccg atcttatttg ctttccacgg
    17401 ttatgaaaca ttggtccgca cgattttctt tgatcgtcat aaccatcatt taatgattca
    17461 tggctacaaa gaaaatggag atatcacgac accatttgac atgcgtgtag taaatgaact
    17521 tgatcgttat catttggcaa aagacgcagc tttaaaaatc aaaggaagtc aagcagaaga
    17581 ttttgcggaa aaaatggatc agaaattaca agaacatcaa aactatattc gtgaaaatgg
    17641 tattgatctg ccagaagtat tggattggaa atggaaaaat cttgaccaat aaaatgataa
    17701 aatcgatccc gcaaaacaga attttttaca ttgtattatt tgtcaaatta ag
//
LOCUS       DACDVT010000051        17459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDVT010000051 DACDVT010000000
VERSION     DACDVT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.051"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            120..527
                     /locus_tag="IWL52_001795"
     CDS             120..527
                     /locus_tag="IWL52_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778341.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            541..942
                     /locus_tag="IWL52_001796"
     CDS             541..942
                     /locus_tag="IWL52_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778342.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            944..1315
                     /locus_tag="IWL52_001797"
     CDS             944..1315
                     /locus_tag="IWL52_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778343.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1351..1653)
                     /locus_tag="IWL52_001798"
     CDS             complement(1351..1653)
                     /locus_tag="IWL52_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778344.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1902..2102)
                     /locus_tag="IWL52_001799"
     CDS             complement(1902..2102)
                     /locus_tag="IWL52_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9778345.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2407..3639)
                     /locus_tag="IWL52_001800"
     CDS             complement(2407..3639)
                     /locus_tag="IWL52_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9778346.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3893..4462
                     /locus_tag="IWL52_001801"
     CDS             3893..4462
                     /locus_tag="IWL52_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9778347.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4640..5080)
                     /locus_tag="IWL52_001802"
     CDS             complement(4640..5080)
                     /locus_tag="IWL52_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9778348.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5238..6002
                     /gene="map"
                     /locus_tag="IWL52_001803"
     CDS             5238..6002
                     /gene="map"
                     /locus_tag="IWL52_001803"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9778349.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6034..6957
                     /locus_tag="IWL52_001804"
     CDS             6034..6957
                     /locus_tag="IWL52_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9778350.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7033..8172
                     /locus_tag="IWL52_001805"
     CDS             7033..8172
                     /locus_tag="IWL52_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9778351.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8165..8965
                     /locus_tag="IWL52_001806"
     CDS             8165..8965
                     /locus_tag="IWL52_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9778352.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8965..9792
                     /locus_tag="IWL52_001807"
     CDS             8965..9792
                     /locus_tag="IWL52_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9778353.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9770..10504
                     /locus_tag="IWL52_001808"
     CDS             9770..10504
                     /locus_tag="IWL52_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9778354.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10604..11470
                     /gene="rfbA"
                     /locus_tag="IWL52_001809"
     CDS             10604..11470
                     /gene="rfbA"
                     /locus_tag="IWL52_001809"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9778355.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11484..12056
                     /gene="rfbC"
                     /locus_tag="IWL52_001810"
     CDS             11484..12056
                     /gene="rfbC"
                     /locus_tag="IWL52_001810"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9778356.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12078..13106
                     /gene="rfbB"
                     /locus_tag="IWL52_001811"
     CDS             12078..13106
                     /gene="rfbB"
                     /locus_tag="IWL52_001811"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9778357.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13204..14055
                     /gene="rfbD"
                     /locus_tag="IWL52_001812"
     CDS             13204..14055
                     /gene="rfbD"
                     /locus_tag="IWL52_001812"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9778358.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14090..16123
                     /locus_tag="IWL52_001813"
     CDS             14090..16123
                     /locus_tag="IWL52_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778359.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16167..17447
                     /locus_tag="IWL52_001814"
     CDS             16167..17447
                     /locus_tag="IWL52_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9778360.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         5465 a         2950 c         3561 g         5483 t
ORIGIN      
        1 tgcttacaaa attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa
       61 ttgactagca aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa
      121 tgtcttatag agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta
      181 atgttattaa taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg
      241 ctttatcatt ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa
      301 tagtagaaat tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat
      361 cggtaagctt gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct
      421 tggttgaaga agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa
      481 ggcaaatctt tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg
      541 atgcttgata tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg
      601 atttttgctt gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact
      661 ccgaaaacta aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt
      721 tcaagtaatg atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa
      781 atcaatataa aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct
      841 gaattgtttt cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat
      901 aaatatatgg ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga
      961 atatttatct atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta
     1021 tagaagaact gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt
     1081 gaaagaaaat tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt
     1141 ttggaatttg ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta
     1201 tagaatgtta gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac
     1261 agatatggcg ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat
     1321 ttcaagcgag tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa
     1381 agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcac
     1441 gatatcaaac aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa
     1501 atagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa
     1561 aacttctgaa ttccatactt tataaaaatc tttgataaat ctattcgaag ggtcaaattt
     1621 aaaccataat tgtgtttttc cttccatcag cataaaagtt cacctcaaaa agagtatacg
     1681 aacgaatgtt ctttttgtga aggcggaatt taatatactg aataatagaa aacaagtact
     1741 tgtgccaaat tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa atagaaaacc
     1801 ttctatatta agatttcctg caataacaaa cacaacagaa gacgtgtaat agttagtcag
     1861 gaacgtacaa ataacccctg tatcctttgc ggtacagggg gttattttgt ataagtagac
     1921 attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct
     1981 tgataaagta aaatagatgc atgcgtatgt cttagtccat caataaacag tcgaagagaa
     2041 aatagggaaa aattgattac taaagaaaaa cttactaggt cgacggtgta gttgattttc
     2101 atacttcaat agattcgaat taaacgattt cgattggctt ataccagtcc tatccaacga
     2161 aagatatatc gttcttaact ctagcggtat aacttgaagc tttacgtaaa gccaatggga
     2221 atgaactgga gatgactttt tattattttt agtaatagct gatgggttaa tacttacaac
     2281 tttccttttc aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa taaaggatct
     2341 aagccaatag cttagatcct ttattctatg ctttatatta ttttttgcat gtgtgcgaaa
     2401 gtatactcaa gcccaatcgc catttcggaa aatcggtact gtagagccat cttcacggat
     2461 tccgtcgata tccatcttgt cagatcctac catgaaatct acgtgggttt gacttctatt
     2521 caatccagct tctgctaatt cttcatcgga catttctgta ccgcctttca cactgaaggc
     2581 ataagcagaa cctagtgcta agtgattcga tgcgttttca tcaaataatg tattgaaaaa
     2641 gatgatgcca gattgagaaa taggtgaagg gtcaggtaca agtgcgactt ctcctaaacg
     2701 acgcgcacct tcatctgtat caagcagttt tgctaagaca tcttctcctt gttcggcaga
     2761 aaaatcaaca acttttccat ctttaaaagt aaatttcata cccgagatga tggtgccagc
     2821 ataactcagt ggctttgtac ttgaaatgta accatctact cgatgactgt caggtgctgt
     2881 gaacacttct tccgtcggca tatttgccat gaatttttct ccacgagcat tatagcttcc
     2941 agcgccttcc caaagatggt ttttaggcaa tccgataatg atatctgtac cgggagcagt
     3001 atagtgaagg gcagaaaatt gttctcgatt caattcttct gctttttttg ctaatttttc
     3061 atcatgcttc ttccatgcta agacagggtc ttcttcatag acacgagtcg ttttgaaaat
     3121 ctgatcccat aatgcctcaa cttgtttttc ttctggcagt tctgggaaga cttttgctgc
     3181 ccattgttta ccagccgcag ccacgacagt ccaactgact ttatttgctt gagttgcctt
     3241 gcgtaaattc attagtgctt tgccattcgc tgattgataa gacgctactc gatggctatc
     3301 tacaccagca aatgcatcgg gatcggcaga aacaacgctg atcctgcttg ctcctttttc
     3361 aagccattca tccgcttgat cgattttaga ttgtggtaca ttctcaatac gatccgttgc
     3421 agcatgtaaa agaaattctc tttggatctg gtcgtcagtc cattgaacga taacctcagc
     3481 ggctcctagt ttataagctt cttgtgtgat caatcgagca aggggtgcct gttccacgct
     3541 gatctgtaaa acaactgtgt gtcctttttc tgtggctacg ccagtttctg caattaagcg
     3601 tgcatatttt tttagaagtt cattaaaatc agataacata ataaaaattc ctcctcacca
     3661 ttttttctgg taactattag catgatacca tttaattatg attcaaacaa tgaggcttta
     3721 aataaaaaaa ggaggttatt tttttaaggt taagtttcat gatatatgtt actacttgct
     3781 atttcagctt gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac atgttacaat
     3841 gaaattaatc aatggtggaa gcgttataaa aaaattaagg agattaaaaa atatggcaag
     3901 aaaaaagacg attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga
     3961 tggatttaca cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc
     4021 gatttatctt gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta
     4081 tgatcattta caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct
     4141 tgcttttaat tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt
     4201 agaagaatat ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac
     4261 agtgaaacgc gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa
     4321 tgggacatgg atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc
     4381 aactgatgaa cagattgaaa agatgatcca agacagtata gatgctatct tgaaattcaa
     4441 agaaccaatc gaaatcgatt gaatatactc aaaagttcca caaatcagta ggtaacaaga
     4501 gatgtgtcaa aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga
     4561 agaactcttc catgaatgca atgaataaaa gagccataca gattgaataa atctggatgg
     4621 ctcttttatt gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat
     4681 tttcgatatc ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg
     4741 cgcctgtttt tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa
     4801 aagtatcacc agatccgcag acaccgtaaa tttttcccaa tagatccaat tcttgcaagt
     4861 cttcataaaa gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta
     4921 ctacgcaaat atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt
     4981 cgacttcgat atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt
     5041 ttcctgtcat gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata
     5101 attatttaac aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga
     5161 cacctgggga atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt
     5221 aaaaaggaga cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag
     5281 aatctggagc tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa
     5341 tcacaagttg ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg
     5401 ctgcgcaaat tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa
     5461 tttgtcatgg attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata
     5521 tgtgtatcga tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat
     5581 ctacacctga gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg
     5641 agcaagcaca agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag
     5701 aaggcgaaaa tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc
     5761 atgaagaacc agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag
     5821 gcatggttat cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc
     5881 ctaacggctg gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat
     5941 tggctattac taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt
     6001 gatctatcca gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa
     6061 aatgaaaatc tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg
     6121 agtacgacct ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt
     6181 gcttttggta atattttacc gtttctccac atcgatcaag agactgtact gacctattta
     6241 cgagaagtga ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg
     6301 acacaaagtt caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt
     6361 caaagtatca atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa
     6421 aattttttga tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt
     6481 agtggggtaa ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc
     6541 gtacaaatac ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg
     6601 gtagctttat ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag
     6661 ttgaagacga tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa
     6721 gtgtttagta tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga
     6781 agttttattg tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga
     6841 atcatcaatg cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat
     6901 ccaagccgaa aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa
     6961 caagagttct tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa
     7021 gtaggtgccc atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta
     7081 ttatcactga tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac
     7141 aaaccaaatg aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc
     7201 tttatttcat ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat
     7261 attgtaccga tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa
     7321 tatgagctga cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat
     7381 tttgtagcag atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta
     7441 ggctggctta gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat
     7501 ctgattgacg gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata
     7561 ggaatcattg gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc
     7621 tgtcttgtag caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat
     7681 ttaggcgata caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg
     7741 ctgaagaacg tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta
     7801 acagacacgt tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca
     7861 gataaaaaac atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc
     7921 ctgacgattt atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact
     7981 ggaagaatcg gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt
     8041 gaattgattg gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta
     8101 gggaataaag agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat
     8161 atagatgaat aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga
     8221 taaaattttt cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa
     8281 aatccgaatt tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag
     8341 gaaatatccc tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca
     8401 taatcacaat ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt
     8461 agtggatgaa gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc
     8521 gatgttagca cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca
     8581 gctggctgtt tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt
     8641 tgacaaacga ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt
     8701 acggatgatc tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg
     8761 gtttgatgag cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa
     8821 agcaggttac aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg
     8881 tgcgcatcga aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa
     8941 taagtggggg tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat
     9001 cagcaactat tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag
     9061 caaattcagc ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa
     9121 aaagaaaata tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat
     9181 tttgcgttgt ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg
     9241 aggctagtcg acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt
     9301 gctgcagttc cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt
     9361 tatatcaatc gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg
     9421 gcaatcaatt caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat
     9481 cctgcttttt ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa
     9541 aaaactgtgg agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa
     9601 aaagtttcaa gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa
     9661 tatgatcaaa ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag
     9721 ttcttgaccg tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat
     9781 tggaaggtgt gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt
     9841 aagagagcag ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc
     9901 tgatgatggg tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa
     9961 gcgaatccag cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat
    10021 ccagcagacg aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac
    10081 aaaagtgaaa aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc
    10141 tgatttaatc attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg
    10201 aaattccaaa gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat
    10261 ggctttccgg tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca
    10321 tgatatgtgg attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac
    10381 tctgtaccga agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca
    10441 aaaaatttct tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg
    10501 ttgataaaag atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata
    10561 ataacaaagt gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct
    10621 tgctgggggc agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat
    10681 gccgatttat gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat
    10741 caaagaaatt ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg
    10801 aaacggaaat gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt
    10861 ggcacaagcg tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt
    10921 aggggacaat atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa
    10981 agatacaggc gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt
    11041 agaatttgat gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc
    11101 aaattacgct gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg
    11161 tatcaaacct tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga
    11221 taaaggaaaa ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac
    11281 acatgaatca ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt
    11341 aaaagtggct tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt
    11401 gctggccctt gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc
    11461 cgctgaataa ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa
    11521 tcgtagaaat ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag
    11581 caaaatttgc agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag
    11641 ctgaagctgg tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat
    11701 tgattcgggt agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc
    11761 cgacttatgg tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg
    11821 tacctaaagg atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata
    11881 agtgtgataa ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt
    11941 tagcgattga ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc
    12001 atccgacatt aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat
    12061 taaggagaaa ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct
    12121 ctaatttcgt tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata
    12181 aactgactta tgcaggaaac aaagagaact tggccggact tcctagcgat cgcgtagaat
    12241 tggtcgtagg agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg
    12301 cagttgttca ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat
    12361 ttgtacaaac gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg
    12421 tacgctatca tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc
    12481 tgccaggaca tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt
    12541 caagtccata ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt
    12601 cttttggctt acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata
    12661 ttgaaaaatt tattcctcgc caagtaacga gtatcttaag cggtattcgt ccaaaattat
    12721 atggcgaagg aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt
    12781 ggctgatttt gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag
    12841 aagataataa gaccgtgatg gaattgatct tagaaatgat gggccaacca aaagacgcat
    12901 acgaccatgt aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat
    12961 tgcgagaaga gctaggttgg cagcctgagt ttacgaactt ccgtgatggg ttggctgaca
    13021 cgatcaaatg gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag
    13081 cagcatatgc caaaaatgga caataataca gaggaaattt cactgtatta atggaaaaac
    13141 acatgtgagg caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataac
    13201 catatgtttt tattaactgg aggaaatgga caattaggta cagaattgcg ccatttatta
    13261 gatgaaaaaa atctgccata tgtttcaaca gatgcaaaag acttggatat cacagatgct
    13321 gagaaaacaa tggcttatat tacagaatta aaacccgatg ttattttcca ttgcgcagct
    13381 tacacagcag tagataaagc agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc
    13441 gatggcacaa gaaatgtagc acttgcagca aaagctgtga atgcacaatt cgtctatatc
    13501 agtacagact atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa
    13561 acgaatccac taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag
    13621 attttagaag actattatat catacgaact tcttgggttt tcgggatcta tggacacaat
    13681 ttcgtcttca cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac
    13741 cagtttgggc gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa
    13801 gaaaaagcac catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgag
    13861 ttcgcaaaag aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa
    13921 tttccgcaaa aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg
    13981 ttaggttttg tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa
    14041 aaaatgaata agtaatttcc tttttatcaa atagaatttg gagcatgata tggaaaaaca
    14101 aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt cgaatttggt
    14161 gcttgacctg tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt
    14221 tacttttaat ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga
    14281 cgatgggaaa aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg
    14341 gctggtgcag acaccttttt acctctcaca agctgaagag gacttttcgt tggtgaacga
    14401 ggattattca ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat
    14461 tacggttatc ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact
    14521 ttccttttat tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat
    14581 gatattaacg aagaggaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc
    14641 tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg
    14701 actgatgatt gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt
    14761 gatgatgctt ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca
    14821 tcaagtgatg ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag
    14881 caagaaagtt tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat
    14941 tggcggagtc atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa
    15001 taccttatat ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt
    15061 ttctttcctt acaaattgga aaattccttt tgaagatatc aactattcga ataactctga
    15121 agtggccctg ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt
    15181 tggcaaaaaa gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt
    15241 ttctatcttt tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc
    15301 ttatgttcct ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat
    15361 ctggtttatc gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt
    15421 gttggcagta aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta
    15481 tttttcggat tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc
    15541 attatttggc tggagacgct tgttttacct cgcttttgtc gccttagtta tcgtatctgg
    15601 ctttttcgtc aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa
    15661 agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt
    15721 atataacttc accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga
    15781 tatagctgca tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta
    15841 tgctcataca agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga
    15901 tatgttttcg gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat
    15961 cagtaaacga ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata
    16021 tggaccggat aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt
    16081 accacaaaca caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata
    16141 atcctgaaat attagtagaa aggagagtga agataattca agcaatcgta caaaaatttg
    16201 tcaacaaaat gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga
    16261 cagcaggggt aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg
    16321 cttttagtgt ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc
    16381 tttatctagg tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa
    16441 atgagtcttt atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta
    16501 aatggatcga gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc
    16561 tcggaatcac gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct
    16621 gggctccagg caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt
    16681 tgatgactct tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgttc
    16741 gaatcatcgg tgggatagca gtcgttgcag cctttttagg tatttgggtc tgccaatcta
    16801 gaggtgcatt agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga
    16861 tgcgtgtcat tcgtgcgcca tttattgcct acacggttac gattctttta gctttgccga
    16921 tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt
    16981 ggcataaatt ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa
    17041 catttatctt ccagcgagga aaccaatttt taggaaacca caatagttat aattctattt
    17101 tgaatatcta tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg
    17161 gtcgcttaac gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt
    17221 ttgcggtgat gatgcagtca tttatggagg atacattgac ttcataccga tgggttccga
    17281 ttatttatat cctgttggct atggccgttc atcgatatga tgaaccagaa gttacggagg
    17341 aattctatgt tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg
    17401 aacaaccaga tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaat
//
LOCUS       DACDVT010000052        17407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDVT010000052 DACDVT010000000
VERSION     DACDVT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.052"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            146..1624
                     /locus_tag="IWL52_001815"
     CDS             146..1624
                     /locus_tag="IWL52_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9778361.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1901..3961
                     /locus_tag="IWL52_001816"
     CDS             1901..3961
                     /locus_tag="IWL52_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778362.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4044..4613
                     /locus_tag="IWL52_001817"
     CDS             4044..4613
                     /locus_tag="IWL52_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9778363.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4788..5594)
                     /locus_tag="IWL52_001818"
     CDS             complement(4788..5594)
                     /locus_tag="IWL52_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778364.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5732..7246
                     /locus_tag="IWL52_001819"
     CDS             5732..7246
                     /locus_tag="IWL52_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9778365.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7649..8632)
                     /locus_tag="IWL52_001820"
     CDS             complement(7649..8632)
                     /locus_tag="IWL52_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9778366.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8637..9284)
                     /locus_tag="IWL52_001821"
     CDS             complement(8637..9284)
                     /locus_tag="IWL52_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9778367.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9369..10001)
                     /locus_tag="IWL52_001822"
     CDS             complement(9369..10001)
                     /locus_tag="IWL52_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9778368.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10017..10568)
                     /gene="coaC"
                     /locus_tag="IWL52_001823"
     CDS             complement(10017..10568)
                     /gene="coaC"
                     /locus_tag="IWL52_001823"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9778369.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10565..11302)
                     /locus_tag="IWL52_001824"
     CDS             complement(10565..11302)
                     /locus_tag="IWL52_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9778370.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11299..12288)
                     /locus_tag="IWL52_001825"
     CDS             complement(11299..12288)
                     /locus_tag="IWL52_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9778371.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12546..13088
                     /locus_tag="IWL52_001826"
     CDS             12546..13088
                     /locus_tag="IWL52_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9778372.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13105..14190
                     /locus_tag="IWL52_001827"
     CDS             13105..14190
                     /locus_tag="IWL52_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778373.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14202..15008
                     /locus_tag="IWL52_001828"
     CDS             14202..15008
                     /locus_tag="IWL52_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778374.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGERKKGGRS"
     gene            15009..15836
                     /locus_tag="IWL52_001829"
     CDS             15009..15836
                     /locus_tag="IWL52_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778375.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15833..16906
                     /locus_tag="IWL52_001830"
     CDS             15833..16906
                     /locus_tag="IWL52_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9778376.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         5706 a         3050 c         3481 g         5170 t
ORIGIN      
        1 gcaatattct caaaaaaaag ataaaaaatg agaataactg atgaaaaaac aagcataatc
       61 catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa gtaattggta
      121 tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag aggaagatag
      181 agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg tagtgctgcc
      241 taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata tcatagaatt
      301 aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag aagaagcaga
      361 gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa tctactttca
      421 acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa cgatcgaaaa
      481 atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa gagaattagt
      541 ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg gggaagaaat
      601 aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt tttctatgcc
      661 atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg aatatctgtt
      721 caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca ctattagttt
      781 gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg aatactttaa
      841 gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc ttcatatccc
      901 taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca cggaagaaga
      961 atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata tccacatgat
     1021 tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg aaatctctga
     1081 agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg tcaatcgaaa
     1141 aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc tacagttttt
     1201 cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag agaataaacg
     1261 ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt atctcttttt
     1321 attggtcggt atttgtccgg tatcatattt tgaagcaccg attcatgtct gtattgattt
     1381 ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat ttaaaaatta
     1441 caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat ctgattgttt
     1501 acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc cagatgactg
     1561 ggaatatttt ggaaatacca tcatcagaga aaaaaataga catttaactg aaaaaatcag
     1621 ctagtattta tggatggcag ctttgtctca catatttatt agctgattta tgtttttttc
     1681 aaagaaaagg taactagata taactagata taactagata tattacttaa caaaattaaa
     1741 aagtacataa gttattattc cgattttctt gaaacaataa aaaatcaaaa aaaatactct
     1801 cctaatagtc catcttcttt tgattttatt tatataatga aatcatctta tgaaaattac
     1861 tacttcatgt ttagacaaga gtagggagga ctaagaataa atgtgttcaa aaagacgttc
     1921 aaaacaacta aataaacacg cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca
     1981 ctacgttaag tttatgatgc gcacgatgct gatcagttta tctgtaggtg tctatatgca
     2041 tgtacccgtt caggcaacga cacaaaccat caaggtcgta cgtacctctg tttctgagac
     2101 cactgattcc cctatcacta ttactcatac tttcactgat gcaaatggaa aacagctact
     2161 tcctgcattg acttcctata ataaagtcag ttatcctcca acgataaacc actactggtt
     2221 aattcctaac aaaacattat ggacatcgga aaccggaagc agtgtgccgc tttctatctt
     2281 ttccgatatg actgatccta caaaaggtga tcagttaaaa cagatcactg atattttgaa
     2341 tcatgctttg acagatataa aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa
     2401 agattattca cagtttgaaa gaaagaaaat cgatgttctg gcagggacaa aagtttcgat
     2461 atctgatctg gtagaaaggc tgaagaacgc agatggaaca caaggagata tgtccaaagt
     2521 aatttcaaac aatgggaata tcgtggatac gactaataca ggaacatcta ctatttcctt
     2581 gtcctatttt gatccaattg ctttaaaaac aatgactgca tctgctgtga tccatgttcg
     2641 tgctgatcaa acagctatca aaggaaaaga tcttcactta agaacaggag agagttacgc
     2701 ccctaaagat cttgttgaat atgtaacaaa aagcgatgga acttcaggaa ataaagaaga
     2761 aatcaaaatg aacgggacag tagatactac aactccggga gaatatcccg ttgtattaac
     2821 ttatatagac tcgaaaacat caaatgtggc agaaacaaca gctgtagttt atgtagaagc
     2881 atctgctgct tcatcgactg tcatcactca ttttatagat agtcaaacaa ataaagaact
     2941 tcaagcagaa acacatatga gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga
     3001 attttctgat cgagtgccaa atatagattt atcgacttat gaacaaaatg ggaaagtcac
     3061 ttctatttct gattggatgc aagcaaacgg attagacaga aaaagtcact ggtcggagat
     3121 ccttgaagta cttcactcag aactgatgaa taataggaag caatcaggag gggaaattac
     3181 ctttacttat gtttatatac cagacaatcg ttcattagaa ggtcatgatc ttattgttca
     3241 tccaaacgaa aaaataaaca aagaagagtt gatccaatca gcaacaaatt ccttaggtaa
     3301 acccgtaccg ttaaaggatg tatcggttac aatcaatgaa gtaccattgg aggatggtta
     3361 tttgaccaaa caaacaggtg attttacgat tatgtatagt tatcatgacc cgtattcact
     3421 ccaagaactc catgctcagt cactattaca tgttttacca ttaaacaaac agatagatca
     3481 aaaagaacaa aaaagacaag aagaaagctt gtcagaaaaa aaacaacaaa tggcccatgc
     3541 agtttctcca acagggaaag taaaacaaac aagaagccta gagttagcaa gaatatctag
     3601 aaaaatctca gaatctaata aaacgataag gcaagaagct catttggccc cacaagtttc
     3661 tggattggaa aatgaaattt tagagcctaa aataaaacaa caatcagaaa aagaatttgt
     3721 tgctaaacag tcggaagatc agaaggaatc agatgaccag aaggaaacga agaaaaaacg
     3781 cttgtcaact attgattcca ccgatgagca tctgcagccg caagtaccac ttcctagtcc
     3841 gggaacaccc gcacctggtg gaggtattcc ttcttctgct ttaggagatt tcatgcgagg
     3901 tatggcagga acatgggtat acgctaatcg aaatgaattt gacgcagatg actggttctg
     3961 atttatatct ctatctgtta tagtcaaata agtagctgag tcatatgtta tttggcataa
     4021 tggagacgag gagggataga aaaatgttag ataaaacgtt accttacgca gaaatctgga
     4081 tgacaagacc acttcaacaa aatgttccaa tcacgccttt agcggaaggt tttgaatttc
     4141 gattttatca aaaagaagat gaatctgctt gggcggagat tgagacttcc gttggggaat
     4201 ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag
     4261 aacttgaaag aaggatgttt tttgtagaga caaaaaaagg agaaaaagta gcaacctgta
     4321 ccgcttggcg aaaaaaatca agagagcaag tgtatccggt ttttcattgg ttagcagtga
     4381 aaccagatta ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc ttgcaagaat
     4441 ttcctaccct ccatacggac ggaccaatct atcttcacac acagacatgg agccatcagg
     4501 cgattgctct ttataaaaaa atgggtttta cttttattgc tgaaaatcta gacggcacac
     4561 ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa attagataaa taacgaaatg
     4621 gtgaaataag tcctcagtat tgggataaaa aacgaaatag tcattcctca tatcttactt
     4681 ttttgagtta atactatcgg ataactaatg gcatgccata cattcaaaaa agaaggggca
     4741 gtgacaaaag tattgtcact gccccttctc ttgtttttta gcatgtttta tcgtttgatt
     4801 tttttaggaa taaaattctt gatgaacgta aatttttggt aatcgtttcg actatagagg
     4861 taaccaaata cactgaaaac aactgctcca ataaaattaa caaataaatc cttcatagta
     4921 tccatgattc ccacgtctaa gtaaccattt tggatgattg ttttatctgt tttaccatct
     4981 gcattttgac tttcaatgat tgtccgagtg atatctggaa catgatagac agtgtttccg
     5041 tctttactta actccacact attgatttgc tggatgatcg tatctttttg catatcgaat
     5101 tgcagtaatt tatctgctga aaattccaca aactcccaca taacaccaac tgtcatcgaa
     5161 aaacagaagg aaacaactgc tagtaataaa ggagtcaagt tcatggtttt tgtattttta
     5221 ttgagcaaat aaacaaggga aaagccgaca cccccagcta aaaatccgct gaatgcatgt
     5281 agaaaagtat cccacattga aaaatagcca taaaaatcac ttaactctcc taaaactgtt
     5341 gaactaaaaa taaaagataa caaaatgatt tctagcatat ttggaatctg tatctttaat
     5401 atcttttcca gaaaataagg gatttcaaat aaaattaatg tcagtaataa aagcattgta
     5461 tttccccagt gttggttcca gacctgatcc actagcataa taacgacgat gatacgtaat
     5521 aaaatataga ttccgtttgt gattctacct gcagtttttg tactgttcgt aaaaaaggtt
     5581 ttcaaatgat tcataaaata actccttcat gtatatcctt aattatacta agaagcgtgt
     5641 gtttaccaaa gctatatgac ttaaagaaaa acagagttga tattgtgaaa aaaatcactt
     5701 aatatagtat aaggagaaaa gggggataca aatgaaaaaa ttagttgctg gtttattaac
     5761 agccgttgca cttcttgcta tgagcgcatg cggcgcagat cagaaaaata cagcgcagac
     5821 aagttctagt acgaaggaat ctgccgaagt atcatcaggc gcttctgaac aagtatatac
     5881 agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt attggagctg gtggtgcagg
     5941 gatggcagca gctatcgaag caaaagatgc tgggttgaat ccagtaattt tggaaaagat
     6001 gcctgttgcc ggaggaaaca catctaaatc atcaagcggt atgaatgctt cagaaacaaa
     6061 atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa
     6121 aggtggtgga ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc actctgctga
     6181 tgcggtcgat tggctagatc aaaacggtat caaactggac aacttaacga tcactggagg
     6241 gatgagtgaa aagaggacac atcgtccgtc tgatggctcg gctattggtg gatacttagt
     6301 ggaggggtta ttgaataacg tccatgagcg ggaaatccct atttttgtca acgcagatgt
     6361 gacagacatt aagaaaaatg gggaaacggt caatgaagtg actgtacatg ttcaaggaga
     6421 agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt ttggtgccag
     6481 caaagaattt attgaaaaat atcgtcccga tttagctgat tatattacga caaatcaaga
     6541 aggatcaact ggagatggta tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat
     6601 ggataaaata caaattcatc caactgtgca gcaggatgag ggggtcttga ttggtgaggt
     6661 tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg aaaagatttg tcaatgaaat
     6721 ggatacaagg gataaagtat ctgcagcaat tacagcattg cctgaaaaat cagcttattt
     6781 gatttttgac caaggcgtgc gtgatcgtgc cacagctatt gaattttatg accaaaaagg
     6841 atatgtcaca actggaaaga caatggaaga attagcagaa aaaatcgatt taccaaaaga
     6901 aacactagca aaaacagttg atggatggaa tacggatgtt gcggagaaaa aagatgctca
     6961 attcaaccgt gccacagcca tggaacatga cttatcgtct ccgaattatt atgcgatcaa
     7021 aatcgcacca ggaatccatt acacgatggg cggtgtgaaa atcaatacga acactgaagt
     7081 attagataaa gaaaaccagc caattagggg gttatatgct gctggtgaag tcacaggtgg
     7141 cttacatgga aacaaccgga tcggtggaaa ttcagttgcg gagatcatta tttttgggcg
     7201 acaagcgggc atacaagcag ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat
     7261 aaaaagaggt tgtgaaatca gcgttttgag ctaataccga gggtcagctg attgaacact
     7321 gtttagtgaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     7381 aaacagcttc ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgaa
     7441 caccgtttat tcggaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
     7501 taaacacgta taaacggcgg aagcagaaga aattccttcg gaaataagct gaaattcaca
     7561 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt
     7621 cccagcctct tttttgatca gcatgatttt attcagttat cttcaaaatg atttttccga
     7681 tgtttttgtt tgcttccata tattcatgcg cttgtttgac tgcgctgaat tggaatgtac
     7741 ggtcaatgat tggccgaatc ttgccatttt ctagaaaagg cccagctttt tgcataaaag
     7801 tttgtgcgag ctctgcttta tactgatcac ttctaggtgt caataatgta ccaagcagtt
     7861 ggacacgttt cccaatcaat acaccaaggt ctatctcagg cacgatggtc ccacctagca
     7921 taccaatcaa gacccaacgt ccatcaacgg ctatagaagc taaattcttt tcccagtaag
     7981 aagcgccgat gaaatccaaa ataacatcga ctccccgacc ttcagtgatt tttcgtacct
     8041 cttcttgaaa gtcttgtttt ttatagttga tcaattcatc tgcaccaagt tctttacaaa
     8101 aggcgagttt ttcttctgat cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag
     8161 ctaattggat cgctgctgtt cccacaccac ttgctcctgc atggatcaag acagtttcgc
     8221 cttcttgtaa ttttccaagc cagtaaagtg tttgataagc agtcaaaaaa acttctgaga
     8281 ttccagcagc agaaacatag tctagcgtat cagggagaag gattgcacga tcggcaggca
     8341 tcacagcata ttcggcataa ccacctagat tgactaatcc gtatactttt gtcccaggag
     8401 taaaagtgga gatatcactt ttattctcaa ctacctctcc agctacttcg actcccaaaa
     8461 tagggtaggg aggtttcaaa gagactgatt ctcttttcat aatatctgtt cgattgacag
     8521 ctgctgtatg gactttgatc agtaattcgc cttgttttgg tttaggaaca ggcacttcaa
     8581 tttgttctaa tacatctaca gaaccaggat gcttgattga aatggcacgc atggatctat
     8641 tcctcctttg cccattcttg gaatgctgta ttcaactctt tgctaccttc agaaagtact
     8701 agagtcgtac gagaaatatc cgtttcttcc actagagcgg caagtacagc agcaactgta
     8761 gagcgagtga tcgtttttga tgccttctca ttaggcttag ttaattgaat ggaagtaact
     8821 tcttcatcgt tagtcaatgt gactggttga agaatgacaa aatctaagtt agttcgtttc
     8881 agccattcat ctgcataatg tttagtaata taataatctg tcatggactc aggccatttt
     8941 gctggttcat ctgcataaac agcactgacc atcagatagc gagagacatg cgcattttca
     9001 gctgcgatca ctgttttgat tgcgccatcc aagtctactt gcagaagatt ctttccttga
     9061 gagccggcag caaaaatcaa tacatcgatg cctttgaatg cttcggtcat tttttccacg
     9121 gaccaagtta aatcaaaaga gacatacgaa acattctctt tttttactaa tgtttgggaa
     9181 gcaacatctc tcacccctgc aaaaatttca tgggaacttg gagctaaatc ttttatcagg
     9241 tgtcggccaa tttgtccgtt tgcacctaca acaaaaattt tcatttggat catcctttcg
     9301 acgtcaaatc tttccattca atagtaacac caaaagagaa aaaaatcaga gatcggctac
     9361 gatgactatc agttagtaag agaagggtgc ttgaagtatc ttaacaagac attgcatact
     9421 gctgcagtcg caagacctgc gacgattcct tcaggtatac cacttgtaaa aataaccgct
     9481 agaatagctg aataaacagc atttaaattt gttccgatga ctttggcata atcttgctgg
     9541 aataaaaagt agatcatatt catcactaaa atcgtattgg tcaatgaacc ggaaagtcca
     9601 gctaagaata aagaaacgga atctgctttt tgtttcatta gtttgcgcag acctttataa
     9661 acgaaataag gaaccacccc aatcaagatg cgaggaataa acgcgatcag caaggctttc
     9721 cagcttccat gttctgttcc gatgactggg ataaacggag aaaagacaaa agacaaaggt
     9781 gtttggataa ctgtgctacg gatcatgctg atcagtccaa atgtaccgcc aagaaaagca
     9841 cctagtcttg gtcctaaaac gatcgaagca atgattacag gaatatgcat ggttgttgca
     9901 ttgattggac cgatagggat aaaacctaaa ggcgtaacag ctaataaaat caaaatagct
     9961 aaaaacatcg cagttaatgt aaagtttttt gtatttttca taagtactcc ttttttctaa
    10021 gcatttcttt cattaagagt cagcatgacc tgttcgatga tcgtctgaat atcagctaag
    10081 gcaccttttc caaaatctcc acaagctaaa agagattctc ttggttcaat ttcctgatag
    10141 ccgacagatc tcaatgtttc cagattacgc tgagtgatcg ggttttgata catattcgtg
    10201 ttcatggcag gggcaatcaa ttttggtgtt cccactggaa gcgccattgc gactgttgaa
    10261 agaagatcgt ctgcaatgcc gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg
    10321 atgagaaaca agtcagcttg ttttgctagt tcgatatgat tgattacctg cggatttgat
    10381 tcttgcatga cgtctgtatg gactgcgcgc ttcgacagag attgcaaggt taatggcgtg
    10441 ataaaatgtg tgctgctttt agacataatc acatctacct gggcacctaa tttgacgaat
    10501 tgacttgtaa gatcagcgct tttgtaagca gaaatacttc cagtaacgcc tagtaagatc
    10561 tttttcattt tgattcctcc agtctaattt tctcagtaat caatgcagca atctcagatt
    10621 tactttgagc ttcttccact gttccatctt tagataagag atagccgtgg tgtgtctcgc
    10681 catggatttc cgtcaaatca tttgcgaaaa taaaatcagt tttgttattt atcagactat
    10741 tttttgcgac tgccagaaga gattcctttg gtacatctac taatagtttg aaaccaatga
    10801 tttttgcttt tggctgccat tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa
    10861 cgagcacaag atgctcggtg ttagaagaga tctttgaaac agtggtatct gtctgttcat
    10921 tttccgcaaa ccaattgtcc agttcttctt gcgttgaaga agtgggtagg ttttttgcca
    10981 gctgttcttc tgaaaaacta aaagcaggag taaaatcact gactgccata ctgtggataa
    11041 tcgcatcgta gtgttctttt ttcgttagtt cttctaattg taaaaataag tccagtgcag
    11101 attcgatcgt ataaatcgtt atattggagt gtcttttcgg tttcagtgct tgtttcgtag
    11161 tcacgtaatc gattgtggta tccggattgg cagctaaata atcagctaat gcttgcccta
    11221 atcgtcctgt cgaatgattt gtaatagcac gtacttgatc gattttttca gtcgttcctc
    11281 cagctgtgat tagaatcttc atttatggtc acctacattt actagtgttc ttccttggtg
    11341 tgttccagca agtagtttag atgctgtttc aggcaattga tccagagtaa tctcttggac
    11401 gaccagttca tctgcaatct gcaaatccgc catctgttgc cataaagaaa gacgttgatc
    11461 tattggtaca ttgacagaat caattccaat caactggatg tttcgcagaa taaaaggcaa
    11521 gacagtcgcg tttagtttga ttcccccagc attgccacat aaagctactg caccgttata
    11581 agagatcaat ggaagaagac tgcttagctg ctcgccacct actgtgtcga tgacatagtc
    11641 gatttgctgt tttcctaaag cttttgttgt ctctggcaga aattcatcag gtcgtacgac
    11701 ttgcaaagca cctagacttt ttagccatgt gacagactct tcttttctgg aaaaggcaat
    11761 gattgactgg aatcctagtt tatgtaacag ggctatcgct gtactgccta caccgccaga
    11821 agcaccagta acgacaactt ttttatcgga ggtcatacct tgacggatta aagcgttcac
    11881 acacaacata gcagtaaacc ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt
    11941 ttttggtaga ggaactaacc agtctcctgg tactttttga tattggctat aaccgcctgg
    12001 atgggtgacg ccaagcccaa aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg
    12061 attgttactt tggacgacag ttccggataa atcaatccct ggagtcatcg gatatgttcg
    12121 tatcacctga ccagactcgc tcattgccaa tgcatcttta taattgacat cagaataggc
    12181 aatttttacc aagacttcat tttcagccaa ctcagtaaat ggcagatcac tgattgctgt
    12241 atggaaaaca ggttccttta ttacctgaaa agatcgaaaa gcgttcattc gtgagacact
    12301 ccttttaaaa ttttctttta tcataccata aagcctactc ttttattctt gttcttccgt
    12361 aaatttagac aaaaattagg tgaaaaataa agaatcaaac aactagaatt tattccaaaa
    12421 gataaagaaa tattcagcaa aaaaaatttt acaaatggtt gcctttttca ataaatttcg
    12481 tatactaata cgagtggtag taaacaggaa gtttactatt agaaaacttc gattcggagg
    12541 aaattatgga aattggtgag aagttaagaa atttgcgcat ccaaaagaat ttgacacaag
    12601 aagaactagg cgaacgcaca gatttgtcaa aagggtatat ttcccagttg gaacgagatt
    12661 taagttcacc atcaatggaa acattctttt ctattcttga agtcttaggt gtgactccag
    12721 aagaattttt tcatcaagaa gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata
    12781 cggtttattt cgatgaagaa aacgggtatg aattggaatg gctcgtggct gattcgaatg
    12841 aaaaagaaat ggagcctgtc ttgctgactt ttgaaggtgc aggagaatat aagacgtttg
    12901 aaccttcatt atctgaaacg tttatttttg tattagaagg ggaattagag ttaagcttag
    12961 gagagacggt ctatgtggca aaagcaggac agtcgatcta ttatcaagcg acaaaacaac
    13021 atcaattaag gaatcatctc aaaaaacgaa caaaagtatt gattgttgca acgcaatcat
    13081 atttgtaata tagggggtaa aaacgtgggg aaaccaatca tttcatttga tcaagtagtg
    13141 aaacgctatg atgatgaaac agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa
    13201 ttttatacac tgcttggtcc ttccggctgt ggtaaaacaa cgatcctacg tattatagct
    13261 ggattcaccg aagctacaga aggggacatc tattttgaag ggaaacggtt gaatgatttg
    13321 ccagctaata agcgacaagt aaatacagtc ttccaagatt atgcactttt tcctcatatg
    13381 aatgtttttg ataacgttgc ttttggattg aagatcaaga aaatgtccaa atcagatatc
    13441 gaaaagaagg taaaagatgc tttgcgtatg gtccagcttc ctggatacga aaaacgggag
    13501 attagtgaaa tgtctggtgg tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat
    13561 gaaccgaaag tattgctatt agatgaaccg ttatcggcgc ttgatttgaa actacgtaca
    13621 gatatgcagt atgaactgcg ggatctgcaa caacgattag gaatcacgtt tatttttgtt
    13681 actcatgacc aagaagaagc gttggcaatg agtgatgaga tttttgtcat gaataaaggg
    13741 aaaatcgtcc aaagtggtac acccgtagat atttatgatg agccaatcaa tcatttcgtt
    13801 gctgattttg ttggggagag caatatcgtg gacggtgtaa tgatcgaaga taatctagtt
    13861 gagtttgttg gtaagcaatt tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt
    13921 gaagttgttt tacgcccaga ggatttgacg atcacgacac cagataaagg aaaactagtc
    13981 gtaacggtcg atactcagct gtttcgtggt gtccattatg aaatcatctg ttatgatgaa
    14041 cagcaaaatg aatggatggt ccattcaacg aaaaaagcta aagaaggaag caaagtagga
    14101 ttggcctttg agccagaaga tatccatgtc atgcgtttca acgagtctga agaagaattc
    14161 gatgcacgtc ttgacagcta cgaagaatag gggggacaag aatgaaaaca atgcgcagga
    14221 tctattccgt cccttatatt ttgtggctcg gtttattcgt gatcgcgcct gtattaatga
    14281 tcatttatca atcttttttt gatatgaatg ggcaattcac gctgaataat tatctatctt
    14341 attttacgtc ggggacatcg ctttctatga cgatcaattc tgtatggtat gcatttttga
    14401 tcacgttttt cacgctgatc atcagttacc cgactgctta ttttttaaca aaactgaagc
    14461 acaagcaatt atggctgatg ctgattattt tgcctacatg ggtgaatctg ctgcttaaag
    14521 cttacgcatt tattgggatt ttcagtgtgc acggcagcgt gaatcatttc tttgatttta
    14581 tcgggatcgg gccgaaacag atattattca cagacttcag ttttctgttc gtggcaacgt
    14641 atatcgagat tccatttatg attatgccaa ttttcaatgc attggaagag atgaatcctt
    14701 ctttgatcag tgctagtaga gatttaggcg cgaatagctt tgagaccttc cgacgagtga
    14761 tttttccgtt atcattaaat ggtgtgaaaa gtggtgtcca agcagtattt attccttctc
    14821 tttctttatt catgctgact cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg
    14881 ctatcgaaca gcatttcttg gttacacaaa actgggggat gggttctaca atcggtgtga
    14941 ttttaatcat tgctatgttc atcgtgatgc tcctgacagg cgagaggaaa aaaggaggtc
    15001 gctcataaat gaaaaaattt aaatggtcgt atctttattt agggatcgtc tttttattct
    15061 tatatgcacc gatcttctat ctgatttttt attccttcaa tgaaggagga acgatgaaca
    15121 gctttacagg atttacttgg gaaaattatg aagctgtttt tgaagataca cgtttgatca
    15181 ttatcgtatt gaatacgttc atgctggctt tcttatctgc attgatcgcc acgatcatcg
    15241 ggacatttgg ggctatggga atctattaca cgaaaaaacg acgtgcacgg acagcgttgt
    15301 tgagcatgaa taacatctta ttagtctctc ctgatgttat tatcggtgct agtttcttga
    15361 ttttctttac actactagga ttcggattag gttttcagag tgtattgctt tcccatattg
    15421 ctttcagcat tccaatcgtc gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt
    15481 caatggtcga tgcggctaga gaccttggtg ctaataattt ccaagtagtc aaaaatatca
    15541 ttttgcctta tctttctcca ggaatcatag ctggatattt tatggcattt acctattctc
    15601 ttgatgattt tgcggttaca tttttcgtca caggaaatgg ctttacgacg ctctcagtag
    15661 agatctattc tcgtgcaaga caaggaatca gtttagaaat caatgcgcta agtgcgttag
    15721 ttttcttgtt ctccatgata ttggtcattg gatactactt tattagtaaa gaaaaccgtc
    15781 caaaacggct gaagaaaaac agaagagtac gcgagggggt ggcacggctg cgatgaaaaa
    15841 attacaatct ctgtttttag gtattctcat cattattgcg gtgctgctgc ttggtgttcg
    15901 tcaacttgaa aaaacaagtg gcatgagtgg agcaaaggta cttaccatct ataactgggg
    15961 agattatatc gatcctagtt tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa
    16021 ttatgaaaca tttgattcta atgaagcgat gttcacaaaa atccagcaag gtgggacaaa
    16081 ctacgacatc gctataccaa gtgaatatat gatccaaaaa atgatcaaag aaaaactagt
    16141 acttccatta gatcattcga agatcaaagg attaaataat attgatccac gatttttaga
    16201 cttggatttt gatccagata atcagtattc gattccatat ttttggggaa ctttagggat
    16261 cgtatataac gataaattct ttgatgcaaa tcaaattaag cattgggatg atttgtggaa
    16321 gccggaatta aaagacagtt tgatgctgat tgacggtgca cgagaagtta tggggctttc
    16381 tttgaatagt ttaggctatt ctttgaatag taagaatatg gcacagctga cagaagcagc
    16441 aaataaattg aatcatttaa caccaaatgt caaagcaatc gtggcagatg agatcaaaat
    16501 gtatatgatc aatgaagaag cctccgtggc agtgacattt tctggcgaag ctgccgatat
    16561 gatgtgggaa aatgaacatt tacattatgt gatcccacca gaaggatcca atctttggtt
    16621 tgataatatc gttatgccaa aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa
    16681 ctttatgcta gaaccggaaa atgcggcaca aaatgcagag tatattggtt attctacacc
    16741 aaatgaagaa gcaaaagcgt tattgccaaa agacatttcg tcggacgaac aattctatcc
    16801 atctgatgac acgatctccc atcttgaagt ctacaaagat ctaggatcga aatacctagg
    16861 catctataat gacctattct tagaattcaa gatgtatagg agataatggc aaaactcagt
    16921 aagaatgggg acttatcaac gttctaaaga gctaaaaatc gtcctttttc tatctgagtt
    16981 ttccaatctt ctaccaaaag cggatgaaac gccgcttttt ttgtgttatt ttattttttc
    17041 taccacgttt ttctaccaga gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg
    17101 ggctagtaaa tctaagattg aaaaagttac agaaggataa agtaattgga aactatgcaa
    17161 atatacttaa cccttctgta atctattaat aaaactaata tatgaatcca gttcaatatt
    17221 tgtagctatg aaaaaacata aaaatcatat tagataaaaa ctaaattggt agataaatta
    17281 ttttgtcaga tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa aaattggaat
    17341 acaaatatag aatagacata ttgcagccga tagtttatta tgcttttaga cacattaaac
    17401 tggagga
//
LOCUS       DACDVT010000053        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDVT010000053 DACDVT010000000
VERSION     DACDVT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.053"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            114..1013
                     /gene="rsgA"
                     /locus_tag="IWL52_001831"
     CDS             114..1013
                     /gene="rsgA"
                     /locus_tag="IWL52_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9778377.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            1026..1676
                     /locus_tag="IWL52_001832"
     CDS             1026..1676
                     /locus_tag="IWL52_001832"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9778378.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            1677..2315
                     /locus_tag="IWL52_001833"
     CDS             1677..2315
                     /locus_tag="IWL52_001833"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9778379.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            2470..3324
                     /gene="mreC"
                     /locus_tag="IWL52_001834"
     CDS             2470..3324
                     /gene="mreC"
                     /locus_tag="IWL52_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9778380.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            3328..3837
                     /gene="mreD"
                     /locus_tag="IWL52_001835"
     CDS             3328..3837
                     /gene="mreD"
                     /locus_tag="IWL52_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9778381.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            4092..5666
                     /locus_tag="IWL52_001836"
     CDS             4092..5666
                     /locus_tag="IWL52_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778382.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(5734..7533)
                     /locus_tag="IWL52_001837"
     CDS             complement(5734..7533)
                     /locus_tag="IWL52_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9778383.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            7656..8162
                     /locus_tag="IWL52_001838"
     CDS             7656..8162
                     /locus_tag="IWL52_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9778384.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            8241..8963
                     /locus_tag="IWL52_001839"
     CDS             8241..8963
                     /locus_tag="IWL52_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9778385.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(9041..10441)
                     /locus_tag="IWL52_001840"
     CDS             complement(9041..10441)
                     /locus_tag="IWL52_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9778386.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(10611..11585)
                     /locus_tag="IWL52_001841"
     CDS             complement(10611..11585)
                     /locus_tag="IWL52_001841"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9778387.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            11938..12498
                     /locus_tag="IWL52_001842"
     CDS             11938..12498
                     /locus_tag="IWL52_001842"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9778388.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            12515..16036
                     /gene="mfd"
                     /locus_tag="IWL52_001843"
     CDS             12515..16036
                     /gene="mfd"
                     /locus_tag="IWL52_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9778389.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            16051..>17344
                     /locus_tag="IWL52_001844"
     CDS             16051..>17344
                     /locus_tag="IWL52_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9778390.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYF"
BASE COUNT         5426 a         3260 c         3688 g         4970 t
ORIGIN      
        1 tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt gagaagtttg
       61 catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg gaggtggcac
      121 ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac gcaaacggag
      181 agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg
      241 gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc
      301 cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga atcattgtca
      361 caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta gttaccttgg
      421 aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca gacgacccag
      481 aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg attgtaccca
      541 atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta tcggtcttta
      601 tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg gaactgaatt
      661 tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg
      721 aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc tcgattgact
      781 ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta gaagcttctg
      841 tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata
      901 gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa ttcttacaag
      961 aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa
     1021 caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta caaagagaca
     1081 ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg gatggccaat
     1141 ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct gttactaaat
     1201 taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca gcatttgcaa
     1261 aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt catcgggcac
     1321 ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca ggaacaccag
     1381 tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg actgtcaatc
     1441 ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg gaactcgctg
     1501 atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga atcgttccag
     1561 aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga tcatatatct
     1621 atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta gactaaatga
     1681 aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat
     1741 acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca ggctacccag
     1801 tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag gaagtatttg
     1861 caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat acacagttag
     1921 gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt ggagctacgg
     1981 gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct cgcttcaaac
     2041 cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat tatttgcctg
     2101 gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc tgcttgactc
     2161 ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa gaagtagaat
     2221 atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt tctttttcag
     2281 atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa aaaaatttgt
     2341 ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa gaggagcaac
     2401 ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta attaattcga
     2461 ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt ggttattgtt
     2521 atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc
     2581 aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac aatctctgcc
     2641 ccagtacggt gggtacgaaa tggagtagct tctgtccata atcttttttc aacttatgcc
     2701 gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca gcaaaacaag
     2761 aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca gacgctgact
     2821 agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg gcaggacatg
     2881 ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt catgtcgcaa
     2941 aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat cgaattactg
     3001 tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc taatggcgaa
     3061 gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt gacccaatta
     3121 actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct tggagggaat
     3181 tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag ttatgggctg
     3241 gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt tgtgacgatt
     3301 gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa gtactatctg
     3361 ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat gctaggggag
     3421 tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt attatgttgc
     3481 agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg ggctatctat
     3541 gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt aattgtatgg
     3601 ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt tttcagtatg
     3661 atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat ttttaaatta
     3721 gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact gctgttgaac
     3781 atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga tgaataattt
     3841 ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag aaatgctaga
     3901 cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat ttttaaaagt
     3961 tgttttgaaa catttttgta acataactat tatatcgttg acacactgct atggtacaat
     4021 ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta agccagtcgg
     4081 aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca atgaccttga
     4141 ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag atacaacagc
     4201 aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca caaatcgatg
     4261 ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta gcaaaacaag
     4321 atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta caagaacgta
     4381 ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta agcaacacaa
     4441 gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct attggacgcg
     4501 ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa caaaaacaag
     4561 ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa gaattagctg
     4621 aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa gctgatttga
     4681 acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag aaagctgact
     4741 tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa caacaacgtc
     4801 tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa gcacgcgaac
     4861 aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc tcagcaacag
     4921 aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct acacaatctt
     4981 cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca gcgcctgaat
     5041 cttcagcaac agaagaatca acaacagcgc ctgaatcttc agcaacagaa gaatcaacaa
     5101 cagtgcctga atcttcagca acagaagaat caacaacagt gcctgaatct tcaacaacag
     5161 aagaatcaac aacacctgcg cctacaacac catcaacaga tcaaagtgtt gatacaggaa
     5221 acggcacagg aagtagtact ccggctccaa cgccaacacc aacacctgaa caaccaaaac
     5281 ctgtaacacc tgctccagct ccttctggaa gtgtgaatgg tgcagcaatc gtagcagaag
     5341 cttacaaata tattggtact ccatatgttt ggggcggtaa agatccaagt ggctttgact
     5401 gctcagggtt cacacgctat gtttacatgc aagtaactgg tcgtgacatc ggtggttgga
     5461 cagttcctca agaatcagct ggaacaaaaa tttcagtttc tcaagcaaaa gctggagact
     5521 tgttattctg ggggtcacaa ggcggaactt accacgtagc gattgcttta ggcggaggac
     5581 aatatatcca tgctcctcaa ccaggcgaaa gcgtaaaagt tggttcagtt caatggtttg
     5641 cccctgactt tgctgtcagc atgtaagaat gattaatcaa aaagagttag cccattctgg
     5701 actaactctt tttttattgt gataaaatag aaatcagata aattcttctg tcaattccat
     5761 gaacgtttgg atatcttcag tcaccatgtc gatacctgct tgccaaaaat ctggttgggt
     5821 aagatcgaca ttcaaatgtt tcttcgcaag atcttccgtc gtcattgagg cagtatcgcg
     5881 tagtaaatgg atatattctt cttcaaaatt tgctcccttt tggttggcgt aagcatagat
     5941 gcccaaacta aagaggtagc caaatgtgta tgggaagtta taaaacggta cgtcatcaat
     6001 aaagaaatgc aattttgcag cccagaaata tggatggtaa gtagctaaag ctccttgata
     6061 gccttctttt tgcgcctcaa ccatcatctc tgtgatctta tcttctgaga ctaatccttc
     6121 ttgccgtgct tcatagaatc gattttcaaa aatgaatcgg gaatggatat tcatgaacat
     6181 agctaaagca ttttgcagtt ttgtatctag caaattgatt ttttcttctt ttgtttttgc
     6241 gacttttaat gtagcatcgg ctacgatcag ttctgcgaat gtacttgctg tttctgcgac
     6301 attcatggca tattcgcgat ttaaagaagg taaatcccac atcgtgctgc tatggaaagc
     6361 atgtcctagt tcatgagcaa gtgtcgcaac ctcattaaca gaattactgt aggtcataaa
     6421 gatccttgat tcttttgttt ctggtaactc agtacaataa ccaccaggac gtttcccagg
     6481 acgatcttct gcttcgatcc agctcttttc aaaagcagat tgggcaaatt tagccatttt
     6541 tggactgaat ttgttaaagt tttcgataat aaatgctgct gcttcgtcaa aactaaaggt
     6601 tttttctttc aaatcgccta aaataatagg tgcatcttga tcttgccatt ccattttttc
     6661 tttaccgaaa agattagctt ttctagtcaa gtaattaaca aggggttgtt tgtttttctg
     6721 gattgtatcc cacatgacag acaatgtttc tttcttcaac cgattgtact ctaatggttt
     6781 ttgtagaaaa tctgtgacac catgaagttc atagtcagaa agtctaaaac catctaaatg
     6841 attcaaagta tccgtgaaga ggggtgcctt ttctttccag gcttcttccc atgctgcgaa
     6901 aagtgtttct ctgacttttg gatctggatc gcccatcatc ttattgaagg cttgtccagc
     6961 agataattcg actacttgtc catcttgttc gaagggaatc gaaatgctgg ctacaatcgt
     7021 atcataatga ctgctccaag catttagacc atctaaagaa agcgtattta taatgttttc
     7081 ttcagcttct gataataatt gtgagccatc acgacgaatc tcgtttaaac gaaaagcaat
     7141 tgtttcaaac gaagattgag aaagcatctg attccaatca ttgtcagata tttctgcaaa
     7201 tttctttgac aatacagttt cggctgattg cagacgaggc agcattgcgt aaagcttgcc
     7261 tgataagacc tttgcatctg aatcattgac attggcagaa agcaatgcag taatatagct
     7321 gctgcattgg gtaaaaccat tcgtaatcgt ttcttgcaat tgaagaatag cgtttaactg
     7381 ctctgcattg tcagaactag gtgaccattt tgttacgcgg tgataatact cgttcatttg
     7441 gtcttccaat tgcttcatcc gttggttcaa agcatcagaa tgactgcctc cggaaaaaat
     7501 agaatctaga ttccaattta atgaataact catgaaaaaa ctcccttcat attgtaatac
     7561 aaaattaagt atagcacttt tttatggaag aagtctctat gaaaatgatt gttgtttctt
     7621 caaaaaaagt ctaagataaa atacagaggt gacttatgaa ttcttcaaaa aaacaaatga
     7681 aaaacggaaa tatttattta gtaatcgcac tgatagtcgt tataatctta ttttacagtt
     7741 cttctcaaac ttacggacag caatcacaag tttcgcgatt agaaagctgg ctgcctaatc
     7801 agccatttaa agacacatta gcgggtatac gttttaatta tggtggaagt atggtgagta
     7861 tcgaccatct gggctacgcc aaatttttag agttcttttt aaggaagggc gcacatttcg
     7921 gtacctattt tattttggga ggcagcttgt ttttaggtgt tttccccaag ctaaaaattt
     7981 ggtggctgac agcgatcctt gcttggcttt cagcaacagg gtacgcaggt ttggacgaat
     8041 tccatcagat gatgaccggt ggacgaacac ctatgttcca agatgtcatg ttggattcga
     8101 tcggtgctct gactgctgtg atattatgtg tgctttttgc ggcattcaag aagcgtttat
     8161 agattggctg tggctttgtt tttttgtgaa atagtactag aatagaagtg tattctcaaa
     8221 agaacaaggg aggaaaaatc atgtcaggac atagtaaatg gcataatatc caaggaagaa
     8281 aaaatgccca agatgcaaaa cgagggaaag tctttcagaa actttctcgt gaaatttata
     8341 tggcagctaa agcaggcggt cctgatccat ctatgaatcc agctttgcgg cttgttgtgg
     8401 acaaagcaaa agctgcaaat atgcctaacg acaatgtgga acgggcgatt aaaaaggcta
     8461 cgagtgccgt agatgaaacg aattacgatg aaatcacgta tgaaggatat ggtccgggtg
     8521 gagtaggaat cttagtccat gcattgacag ataaccgtaa tcggacagcg acaaatgtcc
     8581 gagtagcttt tactcgaaac ggtggatcac tgggagaaac gggttcagtc agttacttgt
     8641 ttgaccgcaa aggatatatc gctattgaac gcgatgggtt gactgttgat gaagagacga
     8701 tgtttgagga tgtattagag gcgggggcag aagatctgca aacttctcct gaagtattcg
     8761 agatttatac tgcaccagaa gattttgctg ctgtacgaga tgagttggaa aaagagttca
     8821 ctttggcgca agcagaattg acgatgattc cacaaacgac gattgattta aatgaggaac
     8881 aaaaagagca attggaacgt ctagtagata agctagaaga cgatgatgat gtttcagaag
     8941 tctttactgc tgctgaaatg taagtagcac attgttagat aataaaaaga aagcacctgt
     9001 accaatcgta cgggtgcttt ctttttatat attatttttg ttagaagacc aatacaggcg
     9061 ttcctttttc gaccatcccg aataattcct tcattacact tggtggggtg ttgatgcagc
     9121 cgtgtgaacc gcgggttttc cataagtctc caccgtattc aggttgccag tctgaatcgt
     9181 ggataccaac acctgtccag tcaatcggca tccagtagtt taccggactc tcgtaaggtg
     9241 ttccatcgtc atttgttcct tttaaagttg catcttcttc tttattccaa acatagaaga
     9301 ctccagcagg tgtaggtgta gttggctttc cggaaacgat atctgtttca agggccactt
     9361 ttccgtcttt gtaataccac atatgctggt tctccagatc aacttcgata tacgtatctt
     9421 cgatcaatgg atgatcagcg gttgtgccac cttgcacaat gggtgaacga gtaaaatctt
     9481 ggcctgccag aatcgctttt ttcaatgctt ctgtctcact gtctgtttgg atcgtccaac
     9541 tgtaagtgcc aactgggacc gtaacttctc ctctttttgt acttttaaat tttgtgtcat
     9601 tcgtacttgt gttatacttt gtgcctaaat ctgttacata ttgacgtact tgttctgtat
     9661 ctaaatcgac ttttccatca ttgtaggtca gccaactcat aatatcactt gatgggatct
     9721 ggaaagtctc accgtttata ctgtaagtag cttttacatt tgcgatagca ttcatggatg
     9781 cgagttgttc tttcagtgag ctgtcttcag atgttacttt tggtttttct ttgaattcag
     9841 tcaattcaat tgtatcttta ccagagttta ccgcactttt taattgtttg accgcagcat
     9901 cgacgtcgat cgtatttcca ttgacttcag gcttgataac gaaagagtca cctgcttttt
     9961 cgatcgttgc atcagtagta ggtgtccggt ctttgttcaa atcagtcagc ttagttgtca
    10021 atgtctgaac ggtagtgtct agtttttggt ggttttgact gacaggatct atttcttgtt
    10081 tttctgccgc tgagacataa gtcattcccc aagtccatcc gttttgttca tttaggatcg
    10141 gagaaagctc cgaagtgaaa tcatctttgt agccgagctc ggattttggt acttcctgcc
    10201 agacttttcc atcttcttta atagccagtt ttttatcaga ataagcttct tttaattttt
    10261 catttgcttt ttcgactgtt agattgctga catttattcc atttatttct gtattcggca
    10321 aaaaatgctt tgtgtagtat gtactgcgat aggtataaaa gctgatcagc actaagagaa
    10381 caccaagaac tgaaagcaaa gcaacatgtt tcgatttttt tcgatgcgaa gatcttgtca
    10441 tatcgacaac tccttaagta aaagtttagg gtggaacggt ctcatgatag cataatttag
    10501 aggaaaagac gaaggttatc tctactttat gaaaaaattt agaaaccata agggtgataa
    10561 aataaaaaaa cggaaaacac cgaatagatg ttttccgtca tgttcaaaga ttattcagcg
    10621 tctagtttag caaaagcttc atctagaatt tcttttaatt gcttagcaga agcagccata
    10681 cggtcttgct cgctgtcact taaaggaatt tcgattactt tttggatacc ttgacggttg
    10741 ataacagcag gtgcaccaat gtagatgtcg ttcaatccat attcgccatt catgaatact
    10801 gataaaggaa gaacagcatt ttcatcatct aagatggcac gagtgatacg tgctaatgct
    10861 actgcgatac catagaaagt agcgcctttt ttctcgataa ttgtgtaagc agcgtcacgt
    10921 acgccaaaga ataggttaac catagcttct tcatcgattt ctgggttgtt ttttacccat
    10981 tcatagattt gcaagccggc aacatttgca tgtgaccaaa ctgggaattc tgaatctccg
    11041 tgttcaccta agatgtaagc atggacgtta cgtgcatcaa cgtcaactaa ttccgcaagt
    11101 gcttgacgga aacgagctga gtcaagagag gtacctgaac cgattacgcg ttctttaggg
    11161 aaacctgaga atttccaagt tgagtaagtc aaaatatcta ctgggttagc ggcaactaaa
    11221 aagataccgt taaatcctga ttcaacgatt tgtgtgacaa catcgcggtt gatttttagg
    11281 tttttgttta ctaagtctaa acgagtttca cctggttttt gtggtgcgcc agctgtaata
    11341 acaactaagt cagcatcatg tgcgtctgcg taagtagcag aatagatttt tttaggtgaa
    11401 gtaaatgcca aagcgtgaga caagtcaatt gcgtctcctt cagttttagc agtattgata
    11461 tccacaatac ctacttcttg agcgatattt tgagtaacta gagcaaaggc atagctagaa
    11521 cctacggcgc cgtctccaac aagaataact ttttgatgat cttttttttc tgcagctgca
    11581 gtcatttagt atcaatcctt ccattgattt gttttttccc atgcttatgc taacatttct
    11641 caagccccat gtcacgctat ttggctataa aatataacag ataacaaggt tgtattatat
    11701 gtaaaagctt acatgtgcgt tatttcacta tttttatata tcaattattt cattaaactt
    11761 tgaagaaatt tttaatttat ttatcattta ttaacgattc tcttttagcc gaaacgcctt
    11821 gttttgtgtt atactatgca ttgacaagta taaaaaagaa aaaagctcga tggactggac
    11881 cgctaattag cagtccagcc ttttgtgctg ctttttagaa ttaggtggaa aacaaaaatg
    11941 aaaatgatcg tcgggttagg aaacccaggt accaaatacc aatatacaaa acacaatatc
    12001 ggctttatgg tcgtggataa aattgcacgc gagcatcaag ctactttcaa aaaaaatccg
    12061 ttcgaagcag aagttgccga attttttcat aatggagaaa agatcctttt agtcaaacca
    12121 caaacattca tgaatgaatc tggtcgtgca gtcggcccac taatgactta ttttgggatt
    12181 tatccagagg aattagtcgt aatctacgat gatttggatc tggcagtggg aaaaatccgg
    12241 ctccgtcaaa agggaagtgc cggcgggcat aacggtatca aaagtattat ttcccatttg
    12301 aatacgaatg tctttgatcg aatcaaggtg ggaatcggca ggccagaagg gaaaaaaaca
    12361 gtagtccagc atgtgcttag tcctttcagc aaagaaaatc agcctttgat agaagaaagc
    12421 atgtgtcaat ctgttaaagc agtcgaatat ctcatagaag ggcattcgtt cgtggatgcg
    12481 atgaatcgat tcaattgagg aaggaggaaa aggaatggat attattcaat tagtcggaaa
    12541 agatccgcaa gtaaaagaat gggtcagcaa tttagaagaa aaaatgccta gacaactaat
    12601 cacaggatta gcgggctcag cgaaaacact tctatttgct cgagcgttta agaaaacaaa
    12661 taaaaacatg attattctga tgccaaattt gtattatgcg aatcagttag cagaagatct
    12721 gcaacatgtg attccgcaag atcagatcca tttatttcca gtggatgaag tgctttctgc
    12781 tgaaatggcc ttttcttctc cagaagcgcg aggcgagaga gttgcaacgc ttaacgcact
    12841 tcaagaaaaa aaagcaggta tctatttgtt gccagtcgca tctttgcaca aacgactgcc
    12901 gaataagaaa acttggctac aggcgcaatt gaattggcat attggtgatg agatagacgt
    12961 ggaaaatctt cctcgccaat tgactttgat gggctatgta cgagaagaga tggtcgcaaa
    13021 accgggagaa ttcagtattc gaggaagcat tatcgatatt tacccgttaa cgacaaccta
    13081 tcctgttcgt gtagaactat ttgatgtaga gatagattct atgcggtatt ttgaagcaga
    13141 aacacaacgc tcaatcgaaa aaacggaaga aatctgggtt tccccaacga gtgaacaagt
    13201 ctacggacca gaggatctag aacatggaat agagcaaatg caaaaaatgc tagaaaaacg
    13261 gcttgctgcg accacagaag aagcagaccg cgagtttttg caagattact tcggtcagct
    13321 gacttcaagc tggcaagcag gtgtcccgac agaagaagca aaattctact cagatcttct
    13381 ttaccaagaa tctgtgacga tcttggatta tttcccttca aatagtttac tagtcgttga
    13441 tgattatcag cgaatcatgg aaacaaatcg ggaaatcgaa agggaagcgg cagaatggca
    13501 gactcaaaaa atcagcgaat tgcgggtgtt ttctgagcag acattttcag cagatataca
    13561 aaaaatcgtg cagaaggaaa agttcaccac aacctttttc tcactcttcc aaaaagggat
    13621 gggaaatctt cgtttccaag caatccataa ttttcaatat cggacgatgc aacagttctt
    13681 cggacaaatg ccattgctga aaacagaaat ggaccgttgg cgcaagcaaa aacagactgt
    13741 tgtgatcttt attccgacaa aggacagaat acgcaaagca gaacaaatgc ttcgagatga
    13801 agacatatta gtagtagaaa cagaaaaaaa tgcattgatc agaggacagg tacaattagt
    13861 tgaaggagct ttgcaggctg gttttgaact tcctcaagaa aaaatagtag ctatcacaga
    13921 aaaagaaatt ttccaaaaaa cgacgaaaaa acaaacgaga cgtcaaacag taacaaacgc
    13981 agagcgattg aaaagctata atgagttaaa agcaggagat tacgttgtcc atgccaatca
    14041 tggtatcggg aaatacattg ggatggaaac gctagaagta gatggcgttc accaagacta
    14101 tatgactatt ttgtatcaaa acgacgacaa actgtttatt cctgttactc aactgaatct
    14161 gatccaaaaa tatgtcgcct ctgaagccaa agcgcctcgc atcaataagc taggcggaag
    14221 tgaatggacg aaaaccaagc gaaaagtctc ttcaaaaatc gaggatatcg ctgacgatct
    14281 aatcaaatta tatgccgcaa gagagtcaga aaaaggatat gcttttggac ctgatgacgc
    14341 ctaccaaaaa gaattcgaaa atgccttccc ctactctgaa actgatgacc agttgagaag
    14401 tgcagcggag atcaaacggg atatggaaaa agaaaaacca atggatcgac tactcgtagg
    14461 tgatgttggt tatgggaaaa cagaagtagc gcttcgagcg gcctttaaag cgataaagga
    14521 aagcaaacaa gtagcctttt tagttccaac aacgatcttg gcacagcagc actatgaaac
    14581 gatgctggaa agatttgaag gtttccccgt aaatatcggg ctattaagtc gtttcagaac
    14641 aagaaagcaa caaaaagaaa ccatcgaaca actgcgtaca ggtcaagttg atattgttgt
    14701 aggaacccac cgaattttgt caaaagatat tgaatttagc gatttgggtc tactgatcat
    14761 tgacgaagaa caacgttttg gtgtaaaaca taaggaacga ttgaaacagt tacgtgcaca
    14821 agtggatgtt ttgactctga ctgcaacacc gattccgaga actttgcata tgtcgatgtt
    14881 aggcgtgcgg gatctttcag taatcgaaac accgcctgcg aatcgttatc cgatccaaac
    14941 gtatgttatg gaaaaaaatc ctggggctat tcgcgaagca atccatcgag aaatgggacg
    15001 aggtggacaa gtattctatt tatataatcg ggtagaaacg atcgaacaaa aagtagaaga
    15061 aatccaagaa ctcgttccgg aagcacggat cggttatgct catgggcaaa tgacagaggc
    15121 gcaattagaa aatacgcttt ttgaatttat tgaaggtcaa tatgatgttc ttgtcactac
    15181 aaccatcatt gaaacaggag tagatatccc gaacgcaaat acgttgttcg ttgagaacgc
    15241 tgattatatg ggattatcca cgttatacca attgcgaggg cgggtaggaa gaagcagccg
    15301 tgtcgcatat gcttacttta tgtatgagca gcagaagatc cttaacgaag tcagtgaaaa
    15361 acgtcttcag gcaatcaaag attttacaga actgggatct ggattcaaga ttgcgatgcg
    15421 ggatctttcc attcgcggtg caggtaatct cttaggtgct cagcagcatg gatttattga
    15481 tgcagtcggt ttcgatatgt attctcaaat gctaagcgaa gctgtttctc gtaaacaagg
    15541 aaagaatagt caagtggaga agaccactgt tgagatcgac ctcggtgtcg atgcgtatct
    15601 acctgaaaca tacgtggcag atcaacggca gaaaatcgag atctataaac gaattcgtga
    15661 acttgattcg caagaaatgc tagatgaact agaagatgat ctgctcgacc gttttggaga
    15721 atatccagaa gaagtagcac atctcttgac aattgggcag atcaaaatgg atggcgatcg
    15781 agcgctccta gaaacgataa ggaaacagga ggcgcatata gtctttactc taagtaaagt
    15841 tggaacaaaa cgctatagtg tggaacagtt attcgaagca ctgacagcga caaaactaaa
    15901 agccagtcta ggcgttgaaa aagaacagat ggtgatacgc ttgacgatcc ccaaccaaat
    15961 ggaagaagca gtatggcttc aggaaatcca aaagtttgtc aaagcgttgc gtgagcaaaa
    16021 atatctgtca gcgtaggaag gagcaatgat ttggatcata aagaaatgcg gcgcgtcatg
    16081 caaggagcat ttgtattgac gattgcttct ttcatagcaa aagtattaag tgccctttac
    16141 cgaattcccc tccaaaactt agtcggcgac gaaggttttt atgtttatca gcaagtttat
    16201 ccaatttatg ggatagcaat gactttagct ttatccggtc ttccgcagtt catatcaaag
    16261 tacgttgcag aaagaaagaa tcctttcgag cgaaagcaag ctttgcaaaa tctttatccc
    16321 ttagtatttt ggacaggtgt ctttttgtgg ttgtttgtct ttgcatttag cagctggatt
    16381 gcttggatga tgggagacat acaattgaaa ccgctgatcc aagtagtttc ctttaccttt
    16441 ttgctgatgc ctggtctatc tttttatcga gggaatttcc aaggaagatt cttgatggaa
    16501 ccaactgctg tttctcaagt agttgagcag ttcgttcgtg tttttgtgat tgtctgtagt
    16561 gcgtttgctt ttagaaagct tggatggacg gtgtaccaaa cagggacggc agcaatgagt
    16621 ggagctgttt ttggcggttt atgcgcatat gggatccttc actactatga acaaaagatt
    16681 catggaggag cgttaagctt ccgacggttt ccattgttca aacagccgtc aaagcccttg
    16741 atccggcgtt ttctggtaga aggcggactg gtttcgattt atagtggatt gcttatcttt
    16801 ttccagctga tcgactcttt ctttgtaaaa aatgctttgg aagtttacgg actgtcagaa
    16861 tacggtgcga agatagcaaa aggggtctat gatcgaggtc agcctttagt acaattaggt
    16921 ttagtgattg ccactgcatt aagtgcgaca ttcctgcctg cgttgactcg tcatttgacc
    16981 aaccggatct atcgtcagtt tttacagaca gccaaaatct atcttagatt gacgaccgct
    17041 ttagcattag ctgcgtcttt gggattagcc ttgctgctcc cttatatcaa ttatgcgttg
    17101 tttaaagatt atgcgggaaa tgcagcgctt gtattatttg ttttttccat tgctttcact
    17161 gcagtcatcc aagcttatca aagtattgct caaagcaaga actcgtttcg cccttcttta
    17221 aaaggcgcag gatggggact attggtaaaa ggattgacta cttcttttct gaccggttta
    17281 ttaggaacag caggtgcaag cctgtctacc cttttaggat taggtgtgac gctttggtat
    17341 tttg
//
LOCUS       DACDVT010000054        17034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDVT010000054 DACDVT010000000
VERSION     DACDVT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.054"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWL52_001845"
     CDS             226..894
                     /locus_tag="IWL52_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9778391.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWL52_001846"
     CDS             complement(1094..1480)
                     /locus_tag="IWL52_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778392.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWL52_001847"
     CDS             complement(1452..2834)
                     /locus_tag="IWL52_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9778393.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWL52_001848"
     CDS             complement(2990..4177)
                     /locus_tag="IWL52_001848"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9778394.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWL52_001849"
     CDS             complement(4184..5215)
                     /locus_tag="IWL52_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9778395.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWL52_001850"
     CDS             complement(5231..6685)
                     /locus_tag="IWL52_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9778396.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWL52_001851"
     CDS             6860..7765
                     /locus_tag="IWL52_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9778397.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWL52_001852"
     CDS             7856..8533
                     /locus_tag="IWL52_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9778398.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWL52_001853"
     CDS             8814..9710
                     /locus_tag="IWL52_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9778399.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWL52_001854"
     CDS             complement(9765..11135)
                     /locus_tag="IWL52_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9778400.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWL52_001855"
     CDS             11373..12119
                     /locus_tag="IWL52_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9778401.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWL52_001856"
     CDS             12116..12955
                     /locus_tag="IWL52_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9778402.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYIFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWL52_001857"
     CDS             12956..13906
                     /locus_tag="IWL52_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9778403.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWL52_001858"
     CDS             14047..14799
                     /locus_tag="IWL52_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778404.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IWL52_001859"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IWL52_001859"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9778405.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16353..16961
                     /locus_tag="IWL52_001860"
     CDS             16353..16961
                     /locus_tag="IWL52_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9778406.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5566 a         3167 c         3394 g         4907 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgctagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagt tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacgggt gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatatt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt tatggttttg
    16201 tatcaaaaaa atatttgttt aaatgttaca aatattaaat aattcgttat tttgtcatta
    16261 aatgtcatgt ttcttttacc tatgaaatat ttctaaatga taaagttaat agcgtcgaaa
    16321 gaataaaaaa cggagaaaaa aaggagttta atatgaattc atttaagaaa attgttttag
    16381 gtacaacttt tgctgctggt gctactgcaa tgtttgtagg aactacaaat gctcacgctg
    16441 atgaagttta tacagtaaaa tcaggtgatt cattatctaa gatctcacaa aaatttgcag
    16501 gagacaactc aatgatcgat gcaattgctg aaaaaaattc gatcgcaaac atcaaccgca
    16561 tttatgttgg tgaacaatta acaatcccaa cttcaaatga ttcttcagca acaacagaaa
    16621 acaaaacaac agaaaataca gcatctacaa ctgaaacagc aacgcaagaa catacatacg
    16681 ttgcacctgt tgaaacagtt gaagtagctc ctgctgcccc agctgctgca acagcgccaa
    16741 cttcaagctc tgcaaaagaa tggatcgcac aaaaagaatc aggcggttca tacacagcta
    16801 ctaacggacg ttacatcggc cgttatcaat tagatgcttc atacttaaac ggcgactatt
    16861 cagctgccaa tcaagaacgt gtagcagaac aatatgtaac atctcgttat ggttcatggg
    16921 atgctgctaa aacattctgg ttagcaaacg gctggtacta agccgataaa aggaaaatga
    16981 tgaaaccgag aaacttgttt attcaagttc tcggtttttt tgtgtgaaaa ataa
//
LOCUS       DACDVT010000055        16659 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDVT010000055 DACDVT010000000
VERSION     DACDVT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.055"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..183)
                     /locus_tag="IWL52_001861"
     CDS             complement(<1..183)
                     /locus_tag="IWL52_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778407.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKY"
     gene            complement(368..865)
                     /locus_tag="IWL52_001862"
     CDS             complement(368..865)
                     /locus_tag="IWL52_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9778408.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(995..1705)
                     /gene="budA"
                     /locus_tag="IWL52_001863"
     CDS             complement(995..1705)
                     /gene="budA"
                     /locus_tag="IWL52_001863"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9778409.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1718..3382)
                     /gene="alsS"
                     /locus_tag="IWL52_001864"
     CDS             complement(1718..3382)
                     /gene="alsS"
                     /locus_tag="IWL52_001864"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9778410.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3587..4249)
                     /locus_tag="IWL52_001865"
     CDS             complement(3587..4249)
                     /locus_tag="IWL52_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9778411.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4259..5074)
                     /locus_tag="IWL52_001866"
     CDS             complement(4259..5074)
                     /locus_tag="IWL52_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9778412.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5336..5779)
                     /locus_tag="IWL52_001867"
     CDS             complement(5336..5779)
                     /locus_tag="IWL52_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9778413.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5913..6251)
                     /locus_tag="IWL52_001868"
     CDS             complement(5913..6251)
                     /locus_tag="IWL52_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9778414.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(6239..6616)
                     /locus_tag="IWL52_001869"
     CDS             complement(6239..6616)
                     /locus_tag="IWL52_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9778415.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6840..8033
                     /locus_tag="IWL52_001870"
     CDS             6840..8033
                     /locus_tag="IWL52_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9778416.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(8199..8621)
                     /locus_tag="IWL52_001871"
     CDS             complement(8199..8621)
                     /locus_tag="IWL52_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9778417.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9211..9666
                     /locus_tag="IWL52_001872"
     CDS             9211..9666
                     /locus_tag="IWL52_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778418.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9828..11000)
                     /locus_tag="IWL52_001873"
     CDS             complement(9828..11000)
                     /locus_tag="IWL52_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9778419.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(11206..12635)
                     /locus_tag="IWL52_001874"
                     /pseudo
     CDS             complement(11206..12635)
                     /locus_tag="IWL52_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(12632..14134)
                     /locus_tag="IWL52_001875"
                     /pseudo
     CDS             complement(12632..14134)
                     /locus_tag="IWL52_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(14131..16458)
                     /locus_tag="IWL52_001876"
     CDS             complement(14131..16458)
                     /locus_tag="IWL52_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9778420.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4772 a         3310 c         2910 g         5667 t
ORIGIN      
        1 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
       61 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
      121 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
      181 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
      241 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
      301 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
      361 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
      421 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
      481 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
      541 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
      601 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
      661 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
      721 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
      781 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
      841 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
      901 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
      961 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1021 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     1081 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     1141 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     1201 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     1261 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     1321 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     1381 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     1441 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     1501 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     1561 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     1621 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     1681 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     1741 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     1801 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     1861 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     1921 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     1981 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     2041 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     2101 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     2161 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     2221 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     2281 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     2341 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     2401 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     2461 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     2521 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     2581 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     2641 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     2701 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     2761 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     2821 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     2881 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     2941 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3001 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     3061 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     3121 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     3181 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     3241 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     3301 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     3361 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     3421 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     3481 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     3541 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     3601 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     3661 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     3721 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     3781 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     3841 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     3901 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     3961 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4021 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     4081 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     4141 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     4201 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     4261 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     4321 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     4381 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     4441 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     4501 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     4561 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     4621 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     4681 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     4741 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     4801 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     4861 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     4921 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     4981 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     5041 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     5101 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     5161 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     5221 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     5281 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     5341 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     5401 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     5461 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     5521 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     5581 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     5641 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     5701 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     5761 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     5821 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     5881 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     5941 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6001 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     6061 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     6121 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     6181 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     6241 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     6301 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     6361 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     6421 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     6481 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     6541 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     6601 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     6661 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     6721 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     6781 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     6841 tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata
     6901 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     6961 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7021 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     7081 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     7141 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     7201 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     7261 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     7321 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     7381 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     7441 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     7501 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     7561 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     7621 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     7681 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     7741 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     7801 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     7861 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     7921 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     7981 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     8041 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     8101 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     8161 gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt
     8221 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     8281 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     8341 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     8401 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     8461 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     8521 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     8581 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     8641 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     8701 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     8761 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     8821 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa aacgggatta
     8881 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     8941 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agtgaaaaaa
     9001 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
     9061 actactcaaa gcacaagttc agctcaggct tccagcagca gtaccacgat ctccgaaagt
     9121 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga agagaaacaa
     9181 aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag cggaatctgc tttgtcacaa
     9241 gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcagctat ccaagcgcta
     9301 cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa ttgattccac aatcaaagca
     9361 aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca agcagaacaa
     9421 gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg cgcgacagca agcagcagct
     9481 gaacaggcct ctcagcaggc acaagccgca gctcctgagc aaaataatga acaaactgtc
     9541 tatgtcacac cgaccggttc aaaataccat acacataaat gtggaaatgg tacgtatacc
     9601 cctgccactt taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa atgttatgga
     9661 aactaattca aaagttatac tataataata aaattcttct gaaacataaa aaaatagaag
     9721 ctggcgttcc agatagtaaa ccaaaaatag cagtttacac gtccatttcg tcagcttctc
     9781 tcttttttca tttagtctgt tccctctgct tttttggaca tcttcattca ttctttctca
     9841 atcaaatagt acacctcttt ttcttttcgc agtttatatc ttcctttttt caatttcatt
     9901 gaaaactgtt tcgaatgttt atgttttaca tggattttaa gcaggagctc ctcatgttgc
     9961 aagcgatagt tcctgccttt taaatcagaa aatctcacta gtccgtattc atcagaagct
    10021 ttcacaaatg gaactttttc tctagtaata tagtgtttgc cgttgagcgt ttttacagaa
    10081 aagaccattc cttcgattgg tcgtccgttt gtgttcttta cataaaaaga taaaggatag
    10141 tttcgcttag cgatggccaa agaatctaaa taagcgatga gtagccaaac taagccgatt
    10201 gatccaagta gtataccgat aaaccaaata aaaaatttcc attttttcca ttgatcaatt
    10261 ccttggatcg ctttttttgc ctcttttgct tgataaggaa tacgatgccc cgtcacaagc
    10321 agtcgatgtg tattgaccat ataaggagta caggtaagca aagtcacgag atctctgcct
    10381 ttttggatac ccaaagaatc tacttcatca ggtaagacca cttggatttt ttctacttga
    10441 tacgccaatg tctttccatt gatctggatg aaaaactggt ctcctttttt caacttaggc
    10501 aaatctgtaa ataattttgc ttctgggagt ccacgatgcc cagaaagaac ggcatgggtg
    10561 tttttcccgc cagtcggata agaactttct tccaataatg acgttccttt ttgcaaaaat
    10621 atttccgttg tctgatcgaa tataggtaag ctgacattga tttttgggat ttcgatgact
    10681 ccaagcatat gttccataaa aaaggcatta ctaggtaaat cttttaatgc ttttgcatca
    10741 actgcttggt tgaaactgcc tatccctggt gatacgtttt gctctgccaa ctgttggttt
    10801 ttctttgtca ttttttcctg ctgttttttt atttcctctg aattcttatt cgatgcttgc
    10861 ttttgataat ggatgatcat ttgctgggca agaaaatcat tcagtgaatc ttctacaaaa
    10921 ggataagaaa agatcccgat cccaacgact aaaatgattg ccatgaagat gtccagtatt
    10981 cttattctgc ttttattcat catgtccacc tattttctaa tatgtatatg aagaggttgt
    11041 gaaatcagcg ccttgacctg agtattcgct caacaaacgg caaaatagtt cctcctattt
    11101 tgccgtttgt tgagcgaata ccaagagacc gaccacttgt cacaaccttt ctccccattt
    11161 gaatcactat cgtttattta tcgtatgatt tttgtttcca atagtttaag cttctgtttc
    11221 ttgatggcgt tttttgaagt agatcactgc tcctccaact aaagcagtac ctaccaagat
    11281 gaatacgatg atccccatac caccagttga aggcaagatt cctttaggtt ggttatcgat
    11341 cgttgtttca tcagaagttg ttggatcatc atctactgta aattcagtgt acgcagcttc
    11401 attcttaggt aatgcgtagc cttctggtgc ttttgtttct tgaaggcagt atgccccttg
    11461 tttcagccct tcgattgaaa atttcccttc tgaatcagaa atcaatttca cttcttccca
    11521 gtcagaacct gtgacttcat ctgaccaagc atatgggtat tctcctgagg caagatttgc
    11581 tatttttttc attaatttaa cagttggatc ttttgctctt agaatagaaa attctgcatc
    11641 ggctaatttt tcttgcgtgt tcacatcttg tttgatgaag tggaaatcac cgtagttttg
    11701 gacattttct acagtaatcg tgttatccgt agttgtattt tcccgattga ctacaccgtt
    11761 tggattccca agattggctt catctgcgcc atggatgata ccggttgttt gttgcaattt
    11821 tccgtttttt actataaaag tagtttcttt ttgtggattg gttaattgat aaccatctgg
    11881 tgcttttgtt tcaactaaag tataattgcc gtctattaat ccattgacac tgatggatcc
    11941 ttcagtatca gttgtgaatg tcaacgctga ctcagcagga tcatagagag cgacaccagt
    12001 gttttcatca aaaccggtca cgaattgatt ctctgaattt ttgatctgga attgcgcccc
    12061 tgctaactta tctacccctg aaaagttccc agctttctgg attttgatat tgctttcttt
    12121 gatgacattt ttagcatata ggtgaatcgt gtctaaaggt ttgtcagggt ctgatgaatt
    12181 cgggttataa aagggtaagc ttaacacgat atttggcgac gtttcgacac cgggctttgg
    12241 tgtttctaca aagacgtata cagcatcttt cccatcagat ttacctggaa gagtcaggtt
    12301 agctaatcct tcaccagaag tcgttacact tgcgaagggt gtagcgtctg ctggaactgc
    12361 tgtgtcttta cttggatagt acgtagcagt gatgtcatat acgttgaatt caacgccgtt
    12421 caacggttcg ctattcggaa atgtcaattc cgttccgttg tttatttgat tttctggtgc
    12481 tgaagtgaat ttcaatttat gcaaattaac cgtaacattt tctggtgtag gtgatacggc
    12541 ctagaaacct ctgcagttcc taggccgaga acgaaaggaa gcagcatgat agcagttcct
    12601 aaaaacgtcc atatttttgt tttgatgttt ttcattttat ttccctccta ttgtttcctt
    12661 tttgttgttt tttataaaac caagctgtcc ctgttgaaag gactagcaac aaccctaaaa
    12721 ttgagaaacc tccaagcatc tcttcgcttg tttttggaaa gcttcctcca tttttttcat
    12781 ttgatggacc atttgtctgc atctgaactg gcgtttttcc attattcgtc ggatttgctt
    12841 ttggttcgtt gatgatttcc aatggtatag gatgaagcgg atcataactg cctttttcaa
    12901 cttcaaagag aatggttgat tgactcaata tatatccttt aggcggttta atctccacta
    12961 gttcgtaact tccaaaagac aatccatgga cagaaaatgt tccatcttta ctggaagtaa
    13021 aaatcgttgc tcttgaatca tctttggcta cccaatgatt tatcccgttt tcttgcgcta
    13081 agtattggtc attttttttg actagaaatt gagcatctgc tagtttttgt ttcgtttgcc
    13141 gatccacttt gacaaattgt ttgcctcctg tttggatggc aagatgtgtt tctactgttt
    13201 gattgtcagt cgtcaaacgt acatcgttat caaatgtcgt tttttcactg ttttctttca
    13261 atgtcatttg gtaacggatc gttaattttt tcccagcaaa cgaactaaga tcttttccag
    13321 aatctgataa gacaaaacca tgttttgttt tttgtgtgtg aaatgtatga atcttgtctc
    13381 caccaatcaa aaaatcgatc gtttctggtt ctagccataa ctgaggatca gctgtatctt
    13441 tgatcgtata ggttcctatc tcgttgatgt tctcaggaat tgttacggtg atgagataag
    13501 gaactttatc cccatctgca aaatctgtct gcttattctc tactgttttt gttaatgtga
    13561 gtgcagcagg tattttttgt tcgctctttg gatacaagtg gatggtcgtc aatttatgat
    13621 tagatgaatc tgtcacagga agcacaacga caaatggtgc tgatttttct tttacttaat
    13681 ctgaagtctt tatttccgca aatcggtaaa cagcgtcacg accttgctca tctttgtccg
    13741 atgcactaaa agaagcaatg ccctcttcct cgtctactgt ttttgtcaca ttttctgcca
    13801 gtattttttc actatcactt ttttgtgcta gttttctttg tgcgtcttcc acactacttc
    13861 cttctgaccg cagcttgtaa aattcatccg agacatcata tatagcgaaa gtaacgccgt
    13921 tcaacccttt tactccttga aagttatccg ggatatttat tccgtcgttt tgactgatac
    13981 ttggcatctt atcgctttcg aacattcgat tatgcaaagt aaatgtaata ggtgattctc
    14041 tagctgacac agtcggagaa cctataaaaa agaccagtag gataaaaaag agaacagcaa
    14101 atatatccac acgtacattt tttgtttttt tcatcgctgt tcacctgctt ttttcttacg
    14161 atggataaaa taaaagaagc cagaaaaaat aaagatcagt cctaaaaaga caaagggcag
    14221 tagacctatc ccacctgtcg aaggaagcaa gagagtcgga ttgatcgtca acttagcggt
    14281 atctgttgcg ataatcgtat aattattgtt tacacttcct tcgcctaaac taatcgttac
    14341 tatttgtgat ccaatatctg tccggctgtt aactgttagg gtataatcca atgattcgcc
    14401 taagacaaca ccggtaacgt ggagatttcc aaccgaaatc gagtgacttt gggcatcata
    14461 attaaaggca gcgtcatcga cagtaatcac tgcgttagct ttatagatgg tataagttcc
    14521 tgatgtgttg gctatatcag taaatgacaa cttgggattt gctgctttga ctttgtctat
    14581 tccgctattc gttagtttaa ttgtatagct gccagcatta accatatttt ttgctgattg
    14641 tgcatctgct gtgtaatcca tactgctaag tgccacttgt actggagtaa gcgaactagt
    14701 gacaccctct gctagcttga catggattga cgggacacct gcttttccat catatgtctt
    14761 actgccactg agctctaccg caccgctgat ttgcttaatc gtatagacat cagatgtctg
    14821 cacgatagta aataaatagt ttgttctggc agctagttta gctagtccgc tttttgtcaa
    14881 ttgccaagac gtataggttc caatttcagt aggcgcagtt acttcaactc ccttttcatt
    14941 catcgctttg aaatcagttg gattccatgt gatttgatca atactgagat cattgtttaa
    15001 aagggatgtt gattctgttt tatcattaaa ggcatattta acgatcggta caacagaaag
    15061 accatcatag tacttactgc catttgcatt gattgatact gttgcatttg tcttataata
    15121 taggatgact tcagaaatct gttttccatt tgcgtccttt acgattacat ttccatttgt
    15181 atctaaacta gctaaaacac tcattgctga ttggttcttt gtttctgtcg ctgaaacagc
    15241 tgtaaaggta tatccgttga tagtaggcgt attagtcgtc aggtcttgtg cagcacttcc
    15301 gtaagtaaga ttcattattt tagaaggagc aatcatctgt ccaaaatagt cttgatactt
    15361 aatagtgatt ggtgtttgtt tgcctgtata ggtcaccgta tcgacgttac tttctagggc
    15421 tgtgaaagaa ccgtttgttt gtgcaaaaga atagcccgtt actgctgttg gagtgataac
    15481 cactttgtca cccacatgga ttccagataa tgtagtcccg tacgttccga tttttgctgc
    15541 tccaccattg actgtataag aaaaagttcc tgtccgattc gcattgacct gaaccgttgc
    15601 tgcttgatag gttggaaaat ttgaaacatt tcccttacca tcaacgatct gcccattttt
    15661 gatcgagtac cccgtgaatg gaatatcatt tgctaactgt ataccgattg aaaaagtact
    15721 catacctcct ccggcaggaa gccctcccca tgttgagggt gtaaaagaat aacttttatc
    15781 cgcattgggt gtaactgaat cgatcgtatt tccagcggta gccaccccca tatagatact
    15841 ttttgggaat gtgatagtag taccatttac tgtggagcca ataaacagat tatggatatc
    15901 tggacggtta tcaataaaag cgattgcttt ggttacgcct tgtaacgcag ttttatccgc
    15961 actcatcgat ccgttaaccg caacaatgac tccatctttg gcatatatca ctgtatatcc
    16021 atctggtatg gtatgtccgt tgaaattcgc agtggccgca acagtactaa atgtctggct
    16081 tctacctgtt tctgaataag ctttctcccc tgctgctttt gctgctgtga tttctttttc
    16141 tgtcggatca atcagttgag tcgaggatgt ggttgaggtt gtctcgttgt tgctagactg
    16201 attggtgctt tctatggact gtatttcaga atagggtgga ctatttgtct cctctgtcgt
    16261 ttggttagca aaatttgacg tttctgtata aacgtttaca taaatacttg ataagctccc
    16321 taagaaagta aaaatcaaga tgatcgaaat cataatggga tgtaagtatt ttcttttaga
    16381 tacagctttg agtctagaaa caaaatgttt ttgacatata cgttggattt tattttgagt
    16441 tttcttaatc attttcatcc ctcctctcaa taaaaaatca tcaaataatc ctttacacgt
    16501 tattctaaat ttaattttta ttattttatt gatgtataga aagtttgggg ctttttttct
    16561 ctttttattc taaggttatt ttagtcttgt cctttaatac aaagatttta tttttaaata
    16621 aaaaatactc tttttttacg attattgaaa aaaaaagat
//
LOCUS       DACDVT010000056        16075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDVT010000056 DACDVT010000000
VERSION     DACDVT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.056"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(140..1126)
                     /locus_tag="IWL52_001877"
     CDS             complement(140..1126)
                     /locus_tag="IWL52_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9778421.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1149..1715)
                     /locus_tag="IWL52_001878"
     CDS             complement(1149..1715)
                     /locus_tag="IWL52_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9778422.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(1729..2145)
                     /locus_tag="IWL52_001879"
     CDS             complement(1729..2145)
                     /locus_tag="IWL52_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9778423.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2602..4536)
                     /locus_tag="IWL52_001880"
     CDS             complement(2602..4536)
                     /locus_tag="IWL52_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9778424.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4838..5773
                     /locus_tag="IWL52_001881"
     CDS             4838..5773
                     /locus_tag="IWL52_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9778425.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            5863..6966
                     /locus_tag="IWL52_001882"
     CDS             5863..6966
                     /locus_tag="IWL52_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9778426.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            6979..7932
                     /gene="menA"
                     /locus_tag="IWL52_001883"
     CDS             6979..7932
                     /gene="menA"
                     /locus_tag="IWL52_001883"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9778427.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(8867..10213)
                     /gene="gor"
                     /locus_tag="IWL52_001884"
     CDS             complement(8867..10213)
                     /gene="gor"
                     /locus_tag="IWL52_001884"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9778428.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10347..11588)
                     /locus_tag="IWL52_001885"
     CDS             complement(10347..11588)
                     /locus_tag="IWL52_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9778429.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11606..12529)
                     /locus_tag="IWL52_001886"
     CDS             complement(11606..12529)
                     /locus_tag="IWL52_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9778430.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12750..15242)
                     /locus_tag="IWL52_001887"
     CDS             complement(12750..15242)
                     /locus_tag="IWL52_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9778431.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15249..15716)
                     /locus_tag="IWL52_001888"
     CDS             complement(15249..15716)
                     /locus_tag="IWL52_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9778432.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(15852..15925)
                     /locus_tag="IWL52_001889"
     tRNA            complement(15852..15925)
                     /locus_tag="IWL52_001889"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15889..15891),aa:Arg,seq:acg)
     gene            complement(15983..16056)
                     /locus_tag="IWL52_001890"
     tRNA            complement(15983..16056)
                     /locus_tag="IWL52_001890"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16020..16022),aa:Pro,seq:tgg)
BASE COUNT         4703 a         3351 c         2865 g         5156 t
ORIGIN      
        1 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
       61 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      121 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      181 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      241 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      301 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      361 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      421 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
      481 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
      541 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
      601 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
      661 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
      721 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
      781 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
      841 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
      901 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
      961 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1021 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1081 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1141 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1201 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1261 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1321 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1381 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1441 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     1501 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg
     1561 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     1621 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     1681 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     1741 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     1801 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     1861 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     1921 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     1981 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2041 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2101 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2161 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2221 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2281 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2341 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2401 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     2461 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     2521 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     2581 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     2641 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     2701 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     2761 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     2821 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     2881 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     2941 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3001 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3061 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3121 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3181 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3241 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3301 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3361 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3421 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     3481 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     3541 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     3601 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     3661 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     3721 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     3781 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     3841 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     3901 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     3961 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4021 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4081 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4141 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4201 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4261 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4321 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4381 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4441 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     4501 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     4561 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     4621 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     4681 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     4741 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     4801 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     4861 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     4921 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     4981 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5041 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5101 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5161 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5221 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5281 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5341 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5401 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     5461 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     5521 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     5581 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     5641 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     5701 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     5761 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     5821 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     5881 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     5941 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6001 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6061 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6121 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6181 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6241 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6301 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6361 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6421 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     6481 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     6541 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     6601 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     6661 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     6721 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     6781 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     6841 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     6901 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     6961 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7021 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7081 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7141 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7201 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7261 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7321 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7381 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7441 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     7501 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     7561 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     7621 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     7681 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     7741 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     7801 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     7861 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     7921 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     7981 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8041 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8101 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8161 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8221 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8281 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8341 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8401 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     8461 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     8521 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     8581 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     8641 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     8701 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     8761 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     8821 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     8881 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     8941 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9001 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9061 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9121 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9181 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9241 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9301 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9361 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9421 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
     9481 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
     9541 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
     9601 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
     9661 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
     9721 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
     9781 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
     9841 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
     9901 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
     9961 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10021 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10081 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10141 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10201 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10261 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10321 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10381 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10441 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    10501 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    10561 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    10621 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    10681 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    10741 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    10801 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    10861 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    10921 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    10981 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11041 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11101 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11161 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11221 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11281 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11341 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11401 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    11461 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    11521 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    11581 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    11641 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    11701 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    11761 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    11821 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    11881 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    11941 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12001 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12061 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12121 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12181 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12241 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12301 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12361 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12421 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagta
    12481 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    12541 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    12601 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    12661 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    12721 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    12781 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    12841 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    12901 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    12961 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13021 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13081 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13141 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13201 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13261 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13321 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13381 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13441 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    13501 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    13561 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    13621 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    13681 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    13741 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    13801 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    13861 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    13921 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    13981 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14041 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14101 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14161 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14221 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14281 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14341 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14401 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    14461 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    14521 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    14581 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    14641 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    14701 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    14761 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    14821 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    14881 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    14941 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15001 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15061 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15121 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15181 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15241 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15301 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15361 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15421 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    15481 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    15541 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    15601 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    15661 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    15721 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    15781 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    15841 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    15901 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    15961 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16021 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DACDVT010000057        14972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDVT010000057 DACDVT010000000
VERSION     DACDVT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.057"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..233)
                     /locus_tag="IWL52_001891"
     CDS             complement(<1..233)
                     /locus_tag="IWL52_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778433.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLL"
     gene            501..2510
                     /gene="metG"
                     /locus_tag="IWL52_001892"
     CDS             501..2510
                     /gene="metG"
                     /locus_tag="IWL52_001892"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9778434.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(2989..3576)
                     /locus_tag="IWL52_001893"
     CDS             complement(2989..3576)
                     /locus_tag="IWL52_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778435.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            3751..5073
                     /locus_tag="IWL52_001894"
     CDS             3751..5073
                     /locus_tag="IWL52_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9778436.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            5228..6004
                     /locus_tag="IWL52_001895"
     CDS             5228..6004
                     /locus_tag="IWL52_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9778437.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            5997..6566
                     /gene="rnmV"
                     /locus_tag="IWL52_001896"
     CDS             5997..6566
                     /gene="rnmV"
                     /locus_tag="IWL52_001896"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9778438.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            6559..7443
                     /gene="rsmA"
                     /locus_tag="IWL52_001897"
     CDS             6559..7443
                     /gene="rsmA"
                     /locus_tag="IWL52_001897"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9778439.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            7894..8523
                     /locus_tag="IWL52_001898"
     CDS             7894..8523
                     /locus_tag="IWL52_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778440.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(8647..9756)
                     /gene="ugpC"
                     /locus_tag="IWL52_001899"
     CDS             complement(8647..9756)
                     /gene="ugpC"
                     /locus_tag="IWL52_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9778441.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            9965..10387
                     /gene="mgsA"
                     /locus_tag="IWL52_001900"
     CDS             9965..10387
                     /gene="mgsA"
                     /locus_tag="IWL52_001900"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9778442.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            10710..11246
                     /locus_tag="IWL52_001901"
     CDS             10710..11246
                     /locus_tag="IWL52_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9778443.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(11286..13049)
                     /locus_tag="IWL52_001902"
     CDS             complement(11286..13049)
                     /locus_tag="IWL52_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778444.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(13052..14767)
                     /locus_tag="IWL52_001903"
     CDS             complement(13052..14767)
                     /locus_tag="IWL52_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778445.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         4789 a         2729 c         3039 g         4415 t
ORIGIN      
        1 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
       61 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
      121 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
      181 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
      241 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
      301 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
      361 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
      421 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
      481 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
      541 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
      601 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
      661 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
      721 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
      781 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
      841 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
      901 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
      961 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     1021 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     1081 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     1141 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
     1201 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
     1261 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
     1321 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
     1381 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
     1441 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
     1501 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
     1561 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
     1621 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
     1681 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
     1741 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
     1801 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
     1861 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
     1921 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
     1981 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
     2041 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
     2101 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
     2161 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
     2221 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
     2281 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
     2341 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
     2401 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
     2461 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
     2521 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
     2581 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
     2641 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
     2701 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
     2761 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
     2821 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
     2881 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
     2941 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
     3001 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
     3061 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
     3121 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
     3181 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
     3241 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
     3301 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
     3361 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
     3421 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
     3481 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
     3541 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
     3601 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
     3661 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
     3721 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
     3781 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
     3841 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
     3901 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
     3961 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
     4021 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
     4081 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
     4141 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
     4201 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
     4261 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
     4321 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
     4381 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
     4441 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
     4501 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
     4561 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
     4621 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
     4681 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
     4741 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
     4801 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
     4861 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
     4921 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
     4981 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
     5041 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
     5101 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
     5161 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
     5221 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
     5281 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
     5341 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
     5401 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
     5461 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
     5521 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
     5581 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
     5641 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
     5701 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
     5761 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
     5821 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
     5881 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
     5941 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
     6001 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
     6061 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
     6121 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
     6181 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
     6241 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
     6301 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
     6361 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
     6421 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
     6481 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
     6541 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
     6601 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
     6661 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
     6721 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
     6781 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
     6841 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
     6901 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
     6961 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
     7021 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
     7081 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
     7141 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
     7201 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
     7261 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
     7321 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
     7381 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
     7441 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
     7501 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
     7561 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
     7621 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
     7681 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
     7741 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
     7801 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
     7861 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
     7921 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
     7981 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
     8041 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
     8101 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
     8161 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
     8221 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
     8281 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
     8341 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
     8401 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
     8461 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
     8521 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
     8581 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
     8641 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
     8701 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
     8761 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
     8821 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
     8881 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
     8941 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
     9001 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
     9061 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
     9121 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
     9181 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
     9241 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
     9301 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
     9361 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
     9421 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
     9481 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
     9541 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
     9601 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
     9661 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
     9721 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
     9781 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
     9841 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
     9901 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
     9961 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    10021 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    10081 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    10141 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    10201 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    10261 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    10321 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    10381 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    10441 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    10501 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    10561 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    10621 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    10681 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    10741 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    10801 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    10861 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    10921 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    10981 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    11041 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    11101 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    11161 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    11221 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    11281 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    11341 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    11401 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    11461 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    11521 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    11581 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    11641 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    11701 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    11761 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    11821 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    11881 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    11941 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    12001 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    12061 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    12121 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    12181 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    12241 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    12301 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    12361 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    12421 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    12481 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    12541 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    12601 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    12661 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    12721 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    12781 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    12841 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    12901 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    12961 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    13021 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    13081 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    13141 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    13201 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    13261 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    13321 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    13381 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    13441 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    13501 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    13561 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    13621 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    13681 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    13741 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    13801 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    13861 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    13921 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    13981 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    14041 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    14101 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    14161 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    14221 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    14281 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    14341 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    14401 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    14461 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    14521 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    14581 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    14641 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    14701 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    14761 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    14821 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    14881 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    14941 aacatgccag tctcttcatt cataggaacg tc
//
LOCUS       DACDVT010000058        14757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDVT010000058 DACDVT010000000
VERSION     DACDVT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.058"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1551)
                     /locus_tag="IWL52_001904"
     CDS             complement(<1..1551)
                     /locus_tag="IWL52_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9778446.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKD"
     gene            complement(1602..2423)
                     /locus_tag="IWL52_001905"
     CDS             complement(1602..2423)
                     /locus_tag="IWL52_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9778447.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(2420..3229)
                     /locus_tag="IWL52_001906"
     CDS             complement(2420..3229)
                     /locus_tag="IWL52_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9778448.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(3250..3744)
                     /locus_tag="IWL52_001907"
     CDS             complement(3250..3744)
                     /locus_tag="IWL52_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9778449.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(3756..4244)
                     /locus_tag="IWL52_001908"
     CDS             complement(3756..4244)
                     /locus_tag="IWL52_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9778450.1"
                     /translation="MFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(4321..6939)
                     /locus_tag="IWL52_001909"
     CDS             complement(4321..6939)
                     /locus_tag="IWL52_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9778451.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEK
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKYENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     EYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLKHILSS
                     EMKPVEFNRLIQELIKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKAEEPNYDMKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
     gene            7422..7637
                     /locus_tag="IWL52_001910"
     CDS             7422..7637
                     /locus_tag="IWL52_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9778452.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(7878..8064)
                     /locus_tag="IWL52_001911"
                     /pseudo
     CDS             complement(7878..8064)
                     /locus_tag="IWL52_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            8498..9457
                     /locus_tag="IWL52_001912"
                     /pseudo
     CDS             8498..9457
                     /locus_tag="IWL52_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            9988..10398
                     /locus_tag="IWL52_001913"
     CDS             9988..10398
                     /locus_tag="IWL52_001913"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9778453.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            10391..10846
                     /locus_tag="IWL52_001914"
     CDS             10391..10846
                     /locus_tag="IWL52_001914"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9778454.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            10863..11594
                     /locus_tag="IWL52_001915"
     CDS             10863..11594
                     /locus_tag="IWL52_001915"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9778455.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            11611..12423
                     /locus_tag="IWL52_001916"
     CDS             11611..12423
                     /locus_tag="IWL52_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9778456.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            12571..13758
                     /locus_tag="IWL52_001917"
     CDS             12571..13758
                     /locus_tag="IWL52_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9778457.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            13991..>14757
                     /locus_tag="IWL52_001918"
     CDS             13991..>14757
                     /locus_tag="IWL52_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9778458.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT         4700 a         2946 c         2535 g         4576 t
ORIGIN      
        1 atctttatta tctaaaaatt gataaaaagg catcaacgta gtgtctgatt cttctccatc
       61 atgcatcaat aaattaattt ttccatccac aatcacatca cttaaaaaga ttaacccttg
      121 ctcaagaatc ggtttttgat agtgcttcac tgaataggtt gttttctcct catcaccgat
      181 tccctccaaa aattgctcac caaaaggacc atcgatagtc atttgtttac aaattgattc
      241 ctttagttta caggcaatgt ttttgtatcg cttgctaaga ctcagttgtt ctagggcttc
      301 aaaaatttct gcaagtccaa tacaggcctt ccaaagacaa agattgctgc cggtatgata
      361 ctttccaagt gcaaaggcgt ctgataacca aacagatgga aaaagcattg gttcccctga
      421 ccgacgttga gctaatatgt cctcaataat tcttgaagca ttcagtagaa cctgcggaca
      481 ccgctcaaag aattttgtat ccccagtatg ttcaaaataa agactagcca gtaacgcact
      541 ggataacgaa ttactcagag aatgattgat acctcctgta aattttgaac caggaaactt
      601 aacgccatat tgatcaaacc atagaaccgt tttttgacat aactccggtt caaacatggt
      661 gaatggcaat gatgcgtaat acatatcctt attccaaatt cgattagtag caggatagct
      721 gccccaattg ctgccgacga cctgtccctg tcgattcata ccgaaactag aaaagctttg
      781 ataaagtgca cgacgataga gatggacaac tctatcgttt ggcattgaca gctgcccaaa
      841 tagctgagca aaatagttca atgtttcctt cagccatttt tcgatatcag attcctgaaa
      901 agtcattact tcttcaaaag catttgggtc cacaaaagca atggcgaagc ttgccacttc
      961 ctgttgtgcg atcgtgtcct tatttccttt ttgagaaatc aacacatttt gctgatccga
     1021 atacttctct tggtacaaag gcacatcttt cactgaaact tgaaggcttt cgtgtgtttc
     1081 attcacaatg taaacctgtt gaacaagcat acttaaacgc ttattagcca gtattggagc
     1141 aaaggatact acaatgacct taaacgtcga ataatgatga atatatctag gcaaacagtc
     1201 ttgaatcaca tcgacttcaa tacgatgatc cgattctgac aaatggtaga caatcccgtt
     1261 tacttcaacc tttaaggata acgaattggt ttgagaaagc tgtttattga cccaaacacc
     1321 tggtttttcc tcagttagta aattttctcg ataaaaggtc aacgtttgaa tcgtcccgca
     1381 aacatcaagg tttcctaatg tcttttgatt acttacatcg aaggtcaatc cctttacatc
     1441 aggaatattc cgatagtagc tgtattcgtt tgaagtagtc acatttccgt cgtttaactc
     1501 agcattaaaa aatgggtgtt tgggtgaata aattacttca ttaatagtca tcttttccct
     1561 ccttgctaaa aaaactgcag cctagtagct gcagcttcct actatgcgat tccaagtaat
     1621 gacaataaaa ttcccaagac gaccattcca aaaataattt tgatgacgcc gactttttta
     1681 tctagtaatt tgaaacagct caatgttacc aataacggta caattccttt aaaaatacta
     1741 tcgaggattt cttggacatt cattagttct tcgcccttca ttgaaaagct caaaacggta
     1801 ttgaatttga ccatactgat cgtcattgca cctaccatga tcagacccaa gatactagca
     1861 gcttttgtca gaatttcaat taacccgttc gcatacagct tttcaatata tttagagcct
     1921 aatgaatagc ctaaaacgcc gccatagtat cgcatcaagt aattaggtat attgaacata
     1981 actaaaaaga taattggtgc caaaatattt ccggcattgg ctaagttgat cgctagtccc
     2041 gcggtaacga cacgccaaac tccccagaac aatgaatcgc caattccagc caatggtccc
     2101 atcaaactcg ttttgatcgc gttgatactt tccacgtcaa aaccagcttt ttcagaattt
     2161 tccttctcca tcgatgccgc aatccccatg acaaaggacg cgcaaggaat ggtcgtcgaa
     2221 aaatactgtg tatgacgttc cagcgccgca gctcgatctt cttcactttt gtagaaatga
     2281 ttaatgaagg gcatcataga ataggcgaaa ccaccgccgc ccatcgtcac cggattcacc
     2341 gaagcataca tagtcatcga gcgccaaaac atcttccgaa ccaatttttt ctcatcttta
     2401 gaaattccat cagctaattt cattgaaaga aatcctcctc ttcttggtca agcgttgcat
     2461 ttacagcagc atcactttga actgcatgaa cagatatatt ttttagttgt agatctcttt
     2521 gggctataac aattgcaata atcgttccaa ctgcggcaat tgcaatcaat ggtaactcga
     2581 gatacgccat aacaacggcc ccaaagaaaa accacacagc aatattttta ctccaaagca
     2641 tccgtaacag catcgccatc ccaactgccg gcagcagatt tccagcgact gttaatccgt
     2701 tcatgatgac ctctggaata ttatccaaga cgcttttgac aacatctgct ccaaataaaa
     2761 cggcaaagaa tggaataaga gcataaagcg catagtggac aaaccacata aagaagtgca
     2821 gattgatgat tcctttttga tcaccagatt ttgcaaattt gaaaaattta gcagccatcg
     2881 gtccacatac aaaagaaaaa atcatattca ttaactgtaa tcctaatact gctagtggaa
     2941 agacaatggg aataatcgca tccacgtttc ctcccatttt gattgcgaac gctgtagcca
     3001 gcccagcagc caaagaaggc tccgccgtac tagagattcc gatattgacg acacccataa
     3061 agatggcttc tagtgacgcc ccgacaatca accccgtctg caaatccccc atcaacgctc
     3121 ctgctaacgg agcaacaaca atcggacgac tcagcatgga catcccgaac atttcatgtc
     3181 cagcaactcc aataaaaacg gccaaagcga ctacaaatgc atcatacata atttttctcc
     3241 ttcctttttc taaaaatcac tcaaaataga gatagctgaa cgtttttcca tcgccggtgt
     3301 aggctgaaaa acggtatctg gataaagttc aactaattct tttaagtctt gaatttctga
     3361 cgtcgttaac atcacttcct ttgataaggt tttcttatct tccgatggcg gtgtcatttt
     3421 cccagcattc cccacgttca ttaattcgat ttccgttggg aaagctttcg caacttttac
     3481 cgcatctttg acattaggga ctaaaaccat caagcgcatg tccttcgccc gcggatcttt
     3541 taacaaatga atcgcgtcat caactgtttt aataatgact ttgacattcg atggtgcagc
     3601 catcttcaat gtcatctttt gaatatcgtt ggtagccgcc tcatcgcttg ctactactaa
     3661 cccggttaaa tttagactct ttgtccaagt catcgctact tgtccatgaa ttaatcgttc
     3721 atcaattcgc actgctttaa tcatttttct tcctcctaaa aaaaatcttc ctcttcattt
     3781 actacatgta cctcatcaag aatggtcatc tttaattcct gttggcattt atttaccagt
     3841 tcctgaatat cctcctttgt taattccttg gttgccaata ctatttcgag cagaattggc
     3901 agattaaagc cagacaaaat agttacagca atctctttct ccaaaaaggt tttggtaatc
     3961 ttctgattga cacttccgcc aaataaatcg gtaaaaacaa aaatctgatc ctgctcgttc
     4021 acctggttta caaatacatt caactcctgt tcataatcac actcatccac ataagcatta
     4081 atcaccgtaa tctcttgaac cttccccgtc agtaattgaa tactgctaag aaagcctgaa
     4141 gctaagtgac cgtgagtcgc tattagaatt ttcttcatat ggttaattcc tttctttctg
     4201 ttcactttca tataagcaat tcctgtgcca acttttaaaa acattggttt aatcacgtta
     4261 attcacataa aaaaaagtgt ctaacctgaa aaaatcagat tagacacttt tttagacact
     4321 ttatttttga atatacggtc ctaataattc atagagcagc gaaagctcat aagcattgac
     4381 tttaaaatta tatttcatct ccattgattg aaaaatactt ctcgaaacat aataaaattc
     4441 atcttcatct ggtttcatat cataatttgg ttcttctgct ttttctgtcc gcgccaagaa
     4501 taaacgttcc atcatcaatg ccgtatgcat ataaagattt agttttacgc ggccatttaa
     4561 agtgaaatga tagtaatttt catatttaga aatgactgtt tccacctcac tcatcacgat
     4621 tgttgggttc agaaagttca aacggtctgc gattccttcc aatgaaaaga attttatcag
     4681 ctcctgaatc aaacggttaa attctaccgg cttcatctca gaactcaaaa tatgcttcaa
     4741 attggctgct ccatcaccat ctaaaatatc atatacattg atattaggaa ttgaaaagga
     4801 acgtggaaga tccgaagtcg tgatgaccaa ttttgtcttg tcaaaataag tttcatcatt
     4861 ttggtcgatt aattcacgta gttctctgta ttccttcgtg aaaacttcga tggactcatc
     4921 cgataaatga tgacgaaaaa catccttcag tttatccgag atacccaatc ccgacatgca
     4981 ggaaatgatg atgttgctac tttgagaaaa gccttcaaag tattgaacat tgattgaata
     5041 cttgtctgct gcatcctccg caatctgttt aaatggcgca ttcatcgtca tcttcaggcc
     5101 aatatcaagt gcaatggagg tcgtcaaatt attcagaaga agtaattctc cttttaaatc
     5161 gttcttgatc tgtgtgtaga tttgatttaa agagcccata tctacgatca gcactagact
     5221 atctgtattc acttgccgtt tcacgaaatt ccttgtcgat tcgacaatct ctgacacatt
     5281 actctctact ggcatatcca acgcttcaaa aacataattg ccgcataatt gattcactac
     5341 tgcttgaatg ctgctagccg tactatctcc gtgagcaagc aacaggccct tcagctcgat
     5401 ggattcatct acataatcag ataaagtcac gacaataagc agtcttagaa agttctcgta
     5461 cttttctgag agcggtggta aatgctccaa gaactgatta cataagtaag tcgtccgagg
     5521 tagatttgca gctaatgtac gatttatccc ggttaagtct gtcttatagc ttggggaaat
     5581 gacataggat ctgctaataa tttctgcaac aggtccactc gtcgtcaatc cgtacgtttt
     5641 tcctaaaacg gatacccaga cctttctaaa catctgaatc atcaattttc tactcattga
     5701 taaaaaagaa tccttatcaa tgtgatgcac caaatgctgt acttcattct ttatcgaaga
     5761 aaacttttca ttgaaattac cttcatttat cagctgagta attttttcca aaatcgaatc
     5821 ataatcaaac ggtttgattc gatccgtttt tatcttttga tttttcttta tcaataaact
     5881 agcaatcggc aagctttcta cttcggattc cagcatattt aactgaatca agatctcatt
     5941 tttcccaatc gttaatggct cttcccgtgt tcgactgtac acatccgcac aactgatttt
     6001 aatgatattc ttcagcttac caatattccc actaaaggga tgagataaca ttagagacaa
     6061 agcttctttt gaaacaataa tatctttttg taaaatatcc gcttcatttt gaaaaaataa
     6121 ttgaatcagc tctaaccgtt ctcgaatggg acgatcttca aaagttggca acataacact
     6181 gatttgaata cgtcgatcaa aggtgtctaa taaataatct tcactttttt ccgtcgtggc
     6241 gaagataaaa cgaaccttag cagaatgcca gtttacattt tcaccgactg gacgataatt
     6301 tccagtatca ataaaaacaa agagcttttc ttgattttct ttcgacaaac gatggacctc
     6361 atcaagaaaa agatagccat tgttggctgt tgccaataac ccctctgtat ccttctcagc
     6421 ccccgtaaag gcccctttct tgtatccaaa cagtgttgcc gaaagcagtt cgggattgtc
     6481 cgcataatcg gcacaattta aaatagcaaa tggtgcatct tcttcaataa tttgctcgtg
     6541 gatagcgtat tcaaaaattt ttgtggctaa aaaacttttt ccaacccccg tggctccatt
     6601 tattaataca ctgagtccgt taggcggata tttgacagct gcaatacatt tttgaatgac
     6661 ctctctcaga cttccgtcat gacccaccac agaatgaaag gcttcgttta tttgctccgt
     6721 attatctaca accgcatatt catctaaagg cttccacaaa acaggctttc ccggtaattt
     6781 ctcgaccttt ttatcttcca ctaatctcgt caaataatgg ctggcattct gacgagagat
     6841 acctagattc ttcgaaattt tctcggcagt gacagaagac atattccctg ttagtcgtat
     6901 cagctcatca aaaactcgat ctttagcgct ctttttcatt ttcccctcca gttcaacgtg
     6961 tctagaagca taataaacta tcggctacaa tatgtctatt ctatatttct gttccaattt
     7021 tactttgaaa caaaaataat cgagcttaaa ctgatattag ttataaaact atagttagat
     7081 taccaatctt cctctcatac aataaacttc tctcacctgc atccaaccta cttatgtgaa
     7141 agaataactg ccgattattt atctttttta cagttacttt cgaacataaa aaaatcacct
     7201 tcaaatacta gaaaaactac tatttgatgg tggtttagaa caatttccgt tgattcagta
     7261 tcgtctggtc ccacatcctc tgtctcatta atcacaatag catactccaa aggataaagc
     7321 tggttgatat ctcaatgcct ttcacaccta tcaattttgt cacacttatc ttttcctagg
     7381 ttctattcta tataatctag aaaaacaaag aaggagacac catgaaaaaa ttcattattc
     7441 cttgtatatt tcttttcttg agtattttgt tattttcaac ttccatccta gtcggttctt
     7501 acctcgattc aagcggaatg ctgattgaac cagcgttctt ttgtgttccg ttgggctact
     7561 taacattagg acttgctttc tttactgcct tatacagttt cgtcaaacaa agattattga
     7621 agaaatactc ggcttgattt gcacgtaaat cctctgttga agaaaagtta gttttttcaa
     7681 caacgaacct tcgagtatta agaatggatg ttgatgtaga catcaaacgt ctgcataaac
     7741 attcatttta ttcctccatt accaacaatt attcccaatc gccaaaagaa gcttttttcg
     7801 gaattacctt ctccattttt tcacctgctg gttatattgt cttttgtaat accgatagtc
     7861 ctcttcaatc attttcaata tgcgaaaaga accaatttta acatggcact caaatcacaa
     7921 ttcctagggc ggccaaaaag atacggctcg ttgatttcaa gggtttcaac gagttcattg
     7981 acaaacccag cggtgctatc ttcttttgga ataagaagtt gtgatatcta aagtgagttg
     8041 attcatgtta taatttgtat gcatgatttt gatcctttca agggttgaat gatttagagt
     8101 gcgagtatcc tctcgtactc ttttatttta cctaaaaagc tccagaaatg acgaatgact
     8161 cgtcatttct agagcttttt tcagagaatt atgtcacaat ctctttttgt tatattgata
     8221 ttctatatct ttttttattg gttttattcc cttgttcgtt atagactaaa atccttcttt
     8281 cagcattatt caaatcaaat ggcaacataa tagagaaatt tatagtatgt ataatcatct
     8341 gaattcaatc aaatcaaccg gagtcatata cggagtcaaa ctatcccact gagagtttat
     8401 taaacgcaga ttgtaaaatt aagctagaca actaaaaaag tcatttgtgc tacactcaaa
     8461 atagttccga ccaaagaatt ataaggagtg aagacaaatg acctataccc atcttacaac
     8521 aaatgaactc gtgatgatag aagcctattt caatatgcat caatctgtta ccgtagtcgc
     8581 taagacctca aatcgttcca gacagaccat ctacaatgtg tacaacttcc tgaaacaagg
     8641 ccaatctatc catgactatt accaacaata tcaaaaaaat aaaacacggt gcggccgacg
     8701 acccatttgt ttgccagcag aacaagatgc gtatatccga aacaaggtgg cgcaaggatg
     8761 gacacccgat gtgattatcg gtcgtgctga gttttcaatt tcatgttctg ttcgaaccct
     8821 ttatcgcaag tttaaaacaa actaatttga ttgctcgaaa ctacccatga aaggaaaacg
     8881 gaaacccaac gggcataaag aacgtcgagg gaaacaagcc ttcaaacgga atatttcaga
     8941 acgtctcacg gactatcctg aatttcaaca ggaatttgga catttggaag gcgacactat
     9001 tgtgggtgtc catcataaaa gtgctgtcat cacgctggtt gagcgcttgt caaaagtgat
     9061 tattacttta aaacccgaag gacggaaagc actagatatt gaaaaagtga caactgactg
     9121 gctacaaaag cttccccgaa acttgttcaa atccattacc tttgattgcg gaaaagaatt
     9181 ttcgaattgg aaaaacatga gtaatgctaa cgatatttct atttattttg cagatcctgg
     9241 aacgctgtca caacggggat taaatgaaca ttctaatgga ttgttacgta aagacggact
     9301 tcctaataca atggatttca atcaggttga ccaaaaattt gtggcggcgg ttgcctccaa
     9361 aagaaatcat ataccaagga aatctttaaa ttatcgaaca tcgttagaag tatttttgag
     9421 ttacataagt gaggagcaat tgtctaactt aatttgacat ttagaaaata ataaaccata
     9481 tcccatctat tcaaataata ttttaccgat tgaaagactt gctaatcaaa gcagaatagt
     9541 ttatatagat aaattatttt ctttaaactt atactcattt ttagatattt tataagaata
     9601 cttcatgtct tcgccagtaa tataaagaac atctgtctct ccatttggac ttgggttatc
     9661 tattttgggt gtaacatact atacatacaa ccgatacata aaagatttga catctggaca
     9721 aaacacaatg ctttacttca tttttatagg ctttgtttac accatcacaa aaatcacctc
     9781 ctaatttaca aattcattaa acaaacagaa ataaaaaatc accacgatag ccgcgttcaa
     9841 aaaaatttac aaaagagaat tttagttaaa gtttttagat tcgccccact aattaccaaa
     9901 ggtattttat ttattcttaa tacgaaataa tttattgaca tttagattac caatggtata
     9961 ttatttataa ataaatggag gtggattatg tacacaatta ttcttgcaag ccacaaaaat
    10021 ttagcgaaag gaatgaaaga gacagtcgaa tatatccttg gccctgcacc attcataaaa
    10081 gtaatgactg catacacaga cgataactat gatattgaaa aagatacgaa tattcttctc
    10141 gatggaactg ataaagtcct tgtcatcact gatttacttg gtggtagcgt aaataatcat
    10201 tggatgaatt atgtttacga aaaaaaatta acaaaaaaaa ttacagtaat tgcaggtatg
    10261 actttgtcct taattatgga attaagtatg aacattgaag attataaatt gagagaaaaa
    10321 atttccatga ttattgctga aagccaaaag agcattataa actgttcaga attaatggag
    10381 gtagaagata atgattaaac tattaagaat tgacgatcgc ctgatccacg gtcaagttgc
    10441 aatggcttgg actaagtccg tatctgctga ctacattatt gtagttgatg acgcctctgc
    10501 taatgacaaa atgaaacaaa tgattttatc attagcaaaa cctgtatcaa cgggactaga
    10561 tattattact tcagcagatt ttttaagtac ctttgaaaaa aggaaaaatc aaaatttgat
    10621 gatagtcact agttccccta aaagcgcatt tgaaattgta aacgctatcc caggggaagt
    10681 cgattcaatt aatatcggtg gtttacggta cgccgaggga aaagagaaag ttaacgagta
    10741 tgtggctatc acatcagaag acaaaaacta cttagcaaaa ttaaaggagt ccggcttcaa
    10801 attaattatg caggctactc caactggaaa agcgaagatt tattagaagg tggggaataa
    10861 gaatgattga atgcatttta attggcatag ttgggtattt aacgacaata gatgaaagat
    10921 attttggggc atctatgatg aatcgaccga taatagttgg acctgttgtt ggacttatct
    10981 taggggatct ccatcaaggc atcttgatag gagcagcatt agaagcaatg tttattggca
    11041 ttgtaacgat tggtgctgca ctaccaccag atgtaggtgt agctggtact attgcaacgg
    11101 ctctagcaat aaaaagtgga gcaggtgctg atgtagctgt tacattggcg atgcctttcg
    11161 ctgtattggc acaaggatta aacatgttgg cttttacgat taactccttt accgtttcac
    11221 aaggtagaaa agcaattgaa aaccgcaaat tatcatcttt tcagaagtgg catttttttc
    11281 cattaatcgt tcgcttacct tccgcagtac ttacattttt agtagtatat tttggtgcca
    11341 atgctacaga aagttttgta aatattatgc cagaaaaatt aatgaccggt ttcaccgtag
    11401 cttctggttt actccccgct gttggatttg ccatgctaat tcaaggaatg attgacaaaa
    11461 aatcagttcc ttattttttc attggatacc ttttagcggc atatttagga gtccccgtag
    11521 ttggtgtagc tgttgctgca attatggttg ctttattagt tatccaaact aaacgcaatc
    11581 ctgtaaatat ttaaagggag agtgataatt atgggagatt ctaaattaac taaaaaagaa
    11641 ataaacaagg ttttctggcg ttcatttgct ttacagggat ccgagtcata cactgatatg
    11701 caaggaatgg gatatagttt ttctatatta cctgctttaa acaaaatata tcaagggaat
    11761 gatgaaaaat attatgaagc tgtttaccga aatacggaat tttttaatac aacgcctcat
    11821 atttcaacct ttattatggg tatgtcatta gcacttgaag aagaaaatgc aaaaacagat
    11881 caatttgaaa ctgaaacaat ttcatcgata aaagctgcat tgatggggcc tgtagctggt
    11941 attggtgata gtttttactg gggaacattt cgcgttattg ctgctggcat cggcttatct
    12001 ttagctgccc aaggaaacat tttaggtcca atattgtatt tttcaattta tacgctgtta
    12061 cattttcttg gcagatatgg ctgcttacgt attggatatc ggatgggagc aaacgccatt
    12121 gaaaaagcat ttgagaacaa ttaccttgaa agtttctcgt tagcagcatc tatcgtaggt
    12181 ttaatgtcaa ttggagccat gacctcatct ctggtacgct ttactattcc cgccgtattt
    12241 aaatcaaaag gtggagaaat aaatgttcag caaatgctcg atgatatttt tccaggaatt
    12301 ctctcattgg ggctaacatt ctttgtttac tttctattaa agaaaaaagt aaaggttctt
    12361 tggattatac ttgctatctt cgtctttagt attgttggag tttatattgg attattaggt
    12421 taattaatcc agaataaaac tagatcagac atcaacttaa acgattacgt ccatataatt
    12481 gtgtaaaagt gaagcatcaa aaaagccatt gatctcagtt aggagtacaa tgttttttgc
    12541 agaaaacata ctcgaatagg actgatatca atgactcaag tacattttac ttttgaaagc
    12601 gaagaaattc aagcaattat aaacgaaagc ggtgcgaatg ataccgcaaa aacacttatg
    12661 actatcatgt ttaatcagct gatggaagaa caacgaaatc aatatattca ggcaaatgcc
    12721 tatgaacgtt cagaagaacg gcaatcacaa agaaatggtt actacgatcg ttcgtttaca
    12781 actcgaattg gtacgttaga acttcatgtt ccacgaacca gagacggtaa gtttagtcct
    12841 actatctttg aaagatatca gcgaagcgaa aaagctttga tcgctgccat gattgaaatg
    12901 gtcatttctg gtgtctccac tcgcaaagta actaaaacag ttgaactgct aacggatggt
    12961 gcgactgttt ctaaatcatt cgtttcaaat ctgatgaaac agttggatcc atttgttttt
    13021 gagtggagaa accggagcct agaaggctcg gaatatccat ttttcatgtg tgatgccctg
    13081 tatatgaaag taagggaaaa tcatcgtatt gtctcaaaag gtgtatatat tggtatcggc
    13141 attgattctg atggacgacg tacgattctt ggttttgatg ttcaagacgg cgaatcggaa
    13201 gataattggg ataccgtttt tcaatcgttc gttcaacgag gtttgttcgg tgtaaaactg
    13261 gtcatttctg atgcacacaa agggttagtt aaggctgttc gcaagaactt tttaggtgcg
    13321 agctggcaaa gatgccaagc ccactttttg agaaatattt ttgataagct gccaaagaaa
    13381 gtttcttctg atgtaaaaga cgagttgaag agtatcttta aagcctctga acttgaattg
    13441 acacgtgaaa gaaaagagca cttcttggaa aaatatggtt gtgattcaaa attaagtgct
    13501 gcttgcgaca ttttagaaaa cggctttgaa gatgctattc aaattttgtc ttttcctgaa
    13561 aatatccgcc gaaggattcg tacaaccaat gtgttggaac ggttgaacga agagattcgc
    13621 cggagagaac gagtgattcg gatttttcca aatatcaatt ccatcactcg gataattgga
    13681 acacttttga tggaaaaaga cactgagtgg ctggcttctc cacgaaaata tttagaattt
    13741 aattcgaata acatttagca ccatgaacct gtgcgagtct cttctttctg aggagagaca
    13801 tcaagagtcg ctgcgctact gctcctgacc tctctcctca gaaagaaagg catggcagtt
    13861 aggttcacaa cgcaaaacat tgtgcttttg atgaggttga acttttacac aagattgtgg
    13921 acttgactag gaagtccctt ctgtagaata aagttaacga caaccaattc acagaaaaga
    13981 ggacttccct atgaatgatt ttactacaga aattgtgcaa actctagtca ctaaaggcga
    14041 tttaaatgaa ttattccgtt cgcacttaga aaaagcgata aacacactcc tacggactga
    14101 attaacggct tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc
    14161 gagaaacggt tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat
    14221 acctagagat cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa
    14281 cgatacattg gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat
    14341 tgctgatttg atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat
    14401 gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta
    14461 tgtcgctatt tttatggacg ctacttacat tccactaaaa cgtcaaaccg tatccaaaga
    14521 agcgatttat attgccattg gtatacgaga agacggcact aaagaagtac tgagttatgc
    14581 gattgctcca actgaatcaa catacgtttg gaatgagctg ctacaggata ttaactccag
    14641 aggagttcaa gaagtcttgc tttttattac ggacggctta aaaggcatga aagatactat
    14701 ccatcaaatt tatcctaaag caaaatatca gcattgttgt atccatgtat ctcgtaa
//
LOCUS       DACDVT010000059        14226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDVT010000059 DACDVT010000000
VERSION     DACDVT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.060"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            159..686
                     /locus_tag="IWL52_001919"
     CDS             159..686
                     /locus_tag="IWL52_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9778459.1"
                     /translation="MKNSMYLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDN
                     LQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIF
                     RSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFI
                     LSTEKKSQLLLMNGG"
     gene            690..1328
                     /locus_tag="IWL52_001920"
     CDS             690..1328
                     /locus_tag="IWL52_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778460.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            1356..1784
                     /locus_tag="IWL52_001921"
     CDS             1356..1784
                     /locus_tag="IWL52_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778461.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            2241..3152
                     /locus_tag="IWL52_001922"
     CDS             2241..3152
                     /locus_tag="IWL52_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9778462.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            3369..3806
                     /locus_tag="IWL52_001923"
     CDS             3369..3806
                     /locus_tag="IWL52_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778463.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            3901..4785
                     /locus_tag="IWL52_001924"
     CDS             3901..4785
                     /locus_tag="IWL52_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9778464.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            5009..8149
                     /locus_tag="IWL52_001925"
     CDS             5009..8149
                     /locus_tag="IWL52_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778465.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            8196..8741
                     /locus_tag="IWL52_001926"
     CDS             8196..8741
                     /locus_tag="IWL52_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9778466.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            8769..9224
                     /locus_tag="IWL52_001927"
     CDS             8769..9224
                     /locus_tag="IWL52_001927"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9778467.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            9221..9979
                     /locus_tag="IWL52_001928"
     CDS             9221..9979
                     /locus_tag="IWL52_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9778468.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(10065..10805)
                     /locus_tag="IWL52_001929"
     CDS             complement(10065..10805)
                     /locus_tag="IWL52_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9778469.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            10866..12119
                     /locus_tag="IWL52_001930"
     CDS             10866..12119
                     /locus_tag="IWL52_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9778470.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            12441..12668
                     /locus_tag="IWL52_001931"
     CDS             12441..12668
                     /locus_tag="IWL52_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9778471.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            12791..13117
                     /locus_tag="IWL52_001932"
     CDS             12791..13117
                     /locus_tag="IWL52_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9778472.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            13185..13898
                     /locus_tag="IWL52_001933"
     CDS             13185..13898
                     /locus_tag="IWL52_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9778473.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         4948 a         2308 c         2907 g         4063 t
ORIGIN      
        1 cagatttaaa aaatgacctg gcgcaaataa cagaatatta tcaagaaatc agtgaaaaag
       61 aaccgaaatt tctatttata aaaaacaatc aacccattta tacatttatt ccttctatcc
      121 cctggatcga acattatccg gttgttgaaa tactgacatt gaaaaacagt atgtatttgg
      181 aaagaaatat tctcagcgga gaaatctatc caccgttaaa gatcaagata ggctctttgt
      241 cgaaagaaag gttatttgag ctgattgaag aaagtcagtt acgtgataac ttgcagttat
      301 catttccgta tactcagact gaagagatag ctgtcaaagt gttaagcaga tcatttcatt
      361 acttgataca gatattttta ctcactttct tttctttctt cttgttgagc tatgtcatgt
      421 tatgcatata ttttgtctac aattgtcgga aaatcgctat ctttcgttca agtggttatt
      481 cgctctttga aacgtataaa gatttcttca tgatgaacct catcaaatgg ggaacgacaa
      541 gtgtaatctt cctttttctt atagaaagag agcctaaata tctttttaat atttttttct
      601 ttagcttcat tgattttatt ctttcagttg tttttatttt gtctacagaa aagaaaagtc
      661 agttattgct gatgaatgga ggttaacaat tggagccgtt gatagtgata aaaggtgcga
      721 aaaaaatcat tcgaaacaaa acgattcttc atttggatcg tttcactgcg gatgctgggg
      781 atcttgtgac tgttgttgga gaaagtggag caggtaaaag tacgttattg aatatattag
      841 gattgaatga tcagttcagt tcaggacaat tctatttgtt cgatcaagag acgaaacaat
      901 taactgggag agagaagcgc ttgataaaaa gaaataccat atcttttctt tttcaagatt
      961 ttgggttgat tgaatatgaa acaatcgatt ttaatttaga tattggattg aagttttcga
     1021 atttaaatgt ttcagataag cgaacagcaa agtatgaggc attgaaaaaa gtaaatataa
     1081 acaagaagct aagtacgcct atctacacat tatctggtgg tgagaagcaa agaatcgcct
     1141 gtgcgagagt cttattgaag aatagcaaaa tcattttagc cgatgaaccg actgggtcat
     1201 tggataaaag aaataaggat aaagtagttg aaatcttatc gcaacaagct tcccaaggta
     1261 cgttagttat cgtagtcacg catgatccat atctgaccaa aataggaaac aagaagatca
     1321 tgctttaaaa atcaaaaagg aaaggatgtc gttttatgaa aaaagcagtt attatttgtg
     1381 cactagggat aggttttgtt ggaggaaacg ctatgtcatc tgaagtggaa gcaaattcag
     1441 ttcacggtga aatctattac aaatctttaa agccaggaga gattcatttt gatgctaaag
     1501 gtaatattat taagtcaaat ttaccagtag accgggaaat ttctagaaca gcaattaatc
     1561 atagctctgg aagatgggtt tattatagtg atacaacttg tacgagaaaa acaggtcatt
     1621 ctaaccacta tagtcgtgtc tataatcgtc acggtgcaaa agcgaaagtt ggaaactcta
     1681 ttcgatcagc aaatgcaaca aaaggagtat gggcctatgc aagtgcgaca ggaaatagaa
     1741 ctgacacatt ctcatgttgg tacaatccaa ccggttggta ttaagtatcc aaaagtttat
     1801 ataaaataga aaaaacatat aatgaaacag cgttaaaagt tatttttggt agttttaata
     1861 tattcatttt tagttgtata aaaatatatc ataataaaaa caacaaaaaa atgtctggaa
     1921 aatcgaacat aaaagtgtct gatttgaata tcccagcagc ggaagaagaa aaacaaaatc
     1981 gataacattt gttctaagaa ttagttacgt taaatacgga acgcaagata gacttgaaaa
     2041 acggtaacaa aagaagtagt atattaaggt gtgatgtgct acttgacatt tattctgctg
     2101 agcgaacttt ctggattgtg tagccttata tctattcagg acgtatcgag cagattagta
     2161 aaggtgttct tgatgatttt catcttgaac acctttgttt ttagcaattc gcaaaaaacg
     2221 aaaagtagga gaattgataa atgaaaaaaa gatttttagc acttgccatc gtactaggca
     2281 ctggcttatt gtcaggctgt acgaatgccg gtgaaaaaac agccgttagt tataaaggcg
     2341 gtacaatcag cgaacaagaa gtaatggata gcttgaaaaa aatgcaagga gcagattccg
     2401 ctgtgcaaca gttgatcgtt tatcaagtat ttgaagataa atatggagat gacgtatcaa
     2461 ccaaagaaat cgattctcaa tatgatcaaa caaagaaaca attgggtgat tcatttgatt
     2521 cacaattgaa atctgcaggt tacacagaac aaacattcaa agacagtatc aaacaaagtc
     2581 tagcctttca agaaggtcta aagaaacata tcaaattgac cgatgaagat ttaaaaacgg
     2641 cttgggaatc tttccatcca gaagtggaag ctcaaattat ccaagtagca agtgaagatg
     2701 atgcaaaaga tgtgaagaaa gctgctgata agggagacga tttctctaaa ttagccaaag
     2761 acaagtcaac tgatactacg acaaaagaag atggcggaaa agtgaaattt gattccacta
     2821 ctacaacggt tccggcagaa gtcaaagaag cagcctttaa gttgaaagat ggtcaagttt
     2881 ctgatgtgat cacttctacc aatgcttcaa cttatactac ggaatattat gtggttaaaa
     2941 tggtgaaaaa ccaaaacaaa ggcaacgaca tggataaata caaaaaagaa ttgaaagaaa
     3001 ttgcaacaga cacaaaatta agtgacagta ccttccagaa taaagtaatt ggcgaagtat
     3061 tgaaagacgc taatgtaaaa atcaaagaca aagactttga aaatgttttg agtacattca
     3121 cttcagattc tagtacagca agtagtaaat aagcggtata aataaaaaat atgaactatt
     3181 taaatcaata aaactgttca tctaaatctt tttattagaa ctaaatcaac agtagtttaa
     3241 ttactacttg atttagttct ttttatttta aaaatttttt ttacttagaa tccagcgacc
     3301 ctagatattt ttctgtataa tcttaaataa agaaaacaat atcaaaaaat gcacgtagaa
     3361 gtagataaat ggagtggttt tgcaaatttc ttttatttct agaagttatt cacgtatacg
     3421 gatttgtttt tctcacaaat aaaaaggcag gaagtacaat ggatcatttg tatataaaat
     3481 atattcatca attttccgtt ttggtgttag gcacactaat tatagcgggg attgtcgcat
     3541 ttattattca aggccgtgac atattaacag tacatcaaga gtttttcagt tatgcatttg
     3601 gtattcctct gatcgctata aggtctgata acaaaaagtg gaagaaacgc tattacattc
     3661 tcttgctatt tttgtgtttt tatctgccac ctaagatttt tactttatta ggtatacaat
     3721 gcctatcatt ttcttttatt tcctatatca ttgttttaat tttcctcagt ttcatagtat
     3781 ggatgtttta tcttgtcaaa aaataaatga atgtttcatg aacagtactt catcacagcc
     3841 taattttttt ctttatgcta tcgttatatt aaagaaatca atcagaaggg aaagaaagtg
     3901 atgaaaaaag ttttattagt ggtaaacccc agttcaggcg gtgaacaagc aaaggaattt
     3961 gagcaattag cgatagcaaa attggaatcg gtgtttgatg aagtggtcgt cttgcataca
     4021 aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg ctacggaagg gtatcatagt
     4081 gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa tcagtggaat cgctgaacaa
     4141 gaacatcggc caaatttcgg atttttccca ttgggtacgg tgaatgactt ggctcgagca
     4201 ttagggatac cgctagaacc tgaggaagcg atcaatcatt tttcgattga atcagtcaag
     4261 ccattggata ttggaaaaat aaatgacgac tattttatga atgtcgtcgc aattggctct
     4321 atccctgaat caatcaatga tgtagatgcg gagaagaaga cgaaatttgg aaaactagct
     4381 tatttcatgt caggaatcaa acagttagct tccacacaat cctactcgtt tcacgtagaa
     4441 gttgatggaa agaaggaaga aattgagagc agtaccttgc tgataggact aactaactca
     4501 gtcggcggtt ttgaaacatt acttccaaat gcaaaagtgg atgatggtaa gctccatttg
     4561 gtttatttaa aagattcttc tttgatcgat acgcttaaaa cattgccaga cctattaaaa
     4621 ggagtagatg aatctacgaa aaatctagcc taccttactt gtgagcaatt agttgtgtca
     4681 ttagctgatg aggaagaatt gacgacgaat gttgatggag atgaagggga taaattacct
     4741 gtgacaatca aagtattgcc ctcacattta caggtttatt gttaaaatgg aggttgtgac
     4801 cgaggagttg gcgggagaac accgcttgtt cgggataagg gactgtgaca agacttttgt
     4861 cacagccttt tttttgtaca aaaaagaaat ttacacctaa tagaaaaatt tttccataaa
     4921 aatacatatt ttaaatatga aagtacaatt ggaaatagat taaaacgata aaataaaatt
     4981 gtcaaaacgt cagagaggat ggtggagaat gaatcaatgt gtaaaaaagc tgattgcgtc
     5041 gattttgttt gtcttccctc tcttgatggt gacatccgtt tttgccgatg tagagaaaac
     5101 aaacgaatca acgcaatcag agaatcaaag ttttgacaag actacttttt cagaagaatc
     5161 gattacttct gaaactacag aatctcaaag tgaggcttct actaataaaa ggcaagaaga
     5221 aaataagaca agtgagacct cagaagtagc tgaacaaacg tctgatagca gtgaagcggc
     5281 actggcaaga gctgtttcag cggatgggta cagcgaagct gccacaacag aagaactggc
     5341 gcagcttctc gcagacgaat cagtaactaa gatccgactg attcagcctt tgactttaga
     5401 cagggaatta gagatcaaac gagatatcgt tattgatttt ggtggattcg ctcacaattt
     5461 cggcacacac catatttata tcaatgagat acctcagtcg atcgagttcc agaattttaa
     5521 aggaacagct gcacatccag gtggattgac accaaatatc gatggaaatg ccattatcat
     5581 tgcttatatg accgattttt ggggaagcag atatcatttt acgggagaaa taaaatttac
     5641 cgggacactt gatttgtatg atgggtccaa attaggactg atttacgctc ctcgtgcaac
     5701 tgtcacatta gatggtgttt ccggtgtgtt ggatgtccaa ccagtgaagg aaggactgaa
     5761 tgccgctccg ggtaaagcct atttttgtag aagctatcaa ttgaatgtga tcaatggtag
     5821 tcaactttat ggaccgtatc tagggaaatt ctacggtttt ctagattcag gagatgagac
     5881 tggtagtgga aaaacagcgc caggaatcca tatcttaagc ggttcaaagg tctctcttga
     5941 ttatgatcgg gcagatggcg ttagcgatga aggagaggca gtcgatacgc tacctggtaa
     6001 tgtcatgttt gaagtcagtg gttcgggaag cgaattttcg gtaaatacaa agatcaatat
     6061 taccaatgac aacaatcgcg gaatcatcca aatgagaggt tcaggatcga aagtcactgt
     6121 atcgaatggt gggaaaatca ccatcaatac agcaactacg ggcggatttc gcttgcaagg
     6181 agattctagt caaatccatg tatcaagtgg tggacaaatc tcggtcaaaa tggctggaga
     6241 tgacgatcaa cccgggaaca atgggatgcg ttttgtagga aaaggccttt ctttgatcgt
     6301 agaaggagcc ggatcagtta tcgacatcga taagcagagt gggcgttcca gcgcagttcg
     6361 ttttgagaat ggtacgcaaa agctgacagt aaccaatgga ggctcattga gggtaagaaa
     6421 tacaggtgat ggcaaatctt acgtgaacag actcaatcaa ggaaatcaag cgatccaatt
     6481 ttatgacgct tctggttttc tagaaagtcc aggttcagct gattttacag tcactggaga
     6541 aaaatcaaat atcgatatac gcgcagattc cggagcaact gtagatacaa ctggagatat
     6601 tgatttaaat tttacggcag gtgaaaaaac ttatttagtc atggtcggga aaacagggga
     6661 agataagacc ggcatcatca gtggggatgt gttgaatgtg gacttagaat ctcctaccta
     6721 ttttgatttc caaaacaaac ggattggaag cgagaccaat ggcataggcg gctgggtatt
     6781 ccaaggaaat ggagattctt ctttaaagat cgcccattca gaaatcgctt tatggcgaaa
     6841 aacaggcgca acattcgata actttgaaaa agacccggat tatttttctg atatgacaga
     6901 cttatcgatc aatgggaccg acttaggcaa tttcgtatca agccctagtg caggtttaac
     6961 aaatgaattt gctcagacag gtgccggaat gaaaaattat aatcgaatca gtgccaataa
     7021 tcagctcccg atgattgaat ccttaagagt gccaacgaat gcagataaaa aaatctatgg
     7081 gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga gatgcttgga caggcgaagt
     7141 agaagtgact ttacgtatta cttacgcaga tacgacgaaa tcatctgtag aaattactgc
     7201 tctcacgcaa ggacaaacag atgaaaatga aggggcagga tataacccat ggggagaagg
     7261 cgaacaaggc ggcttgttcg aagttcaagt acccaatgat gagagattaa aaacggagga
     7321 tcaagtgcaa gttgttgctg ctaaaaaagt aagaggtggc caagaagtga cagaactgga
     7381 ctctgccaaa acgacagtcg atgtgattcc tcctgaacct gctaatttaa gtaccacatt
     7441 gatcaatgca gcttctcgca cgatcacagg tacaggtgaa gcaggagcct tggtttcatt
     7501 gaaaaaaaat caagctgttt tgtcagaaac aactattgat gatacagggg aatttgttct
     7561 ttctatccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat taaacgacca
     7621 agcaggagaa gctactgcta agggtgtgca gaacaagcca agtaccaacg atgaaattgg
     7681 gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt ttgcacctgc
     7741 tacgaagatc gtagtagaag gtggtctctc atttcttgcc ccttccaaac tcgattttgg
     7801 tcagatcaaa gcaactggtt tgacacaaga agcatttggc caaatggata tacaggaaaa
     7861 attatggatt tatgatcagc gagaaacgaa aaatccgtgg atcttaacga taaaattatc
     7921 tcagcctttc acaaatgatg caggattttc tatgcaggat gtcctatatt tcaagaaagg
     7981 agaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac aaaattcaac
     8041 gaatgtgcaa gcagattatt cagaatattt aaataacggt cgggcgttta aacttgtctt
     8101 aaccaaagaa aatcagatag ctggtaatta ctcagcagag ataacataga cattagcaga
     8161 tgcgccgaca gaataaagat caaggaggat gattgatgaa tcgaatatgg aataaaagtc
     8221 atcgaaacaa ctgctcttta cttatgatcc ttctccttgg attcatcctt tggcagacac
     8281 ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct aatcagattg
     8341 ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag gaaatcccct
     8401 acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc cactccgcta
     8461 cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt gatgaaacat
     8521 taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtt cctcctcaaa
     8581 gtgcggtcac tgagacactt tcactgaaag cgccagaaaa agagctggct ggtgtggtcg
     8641 caggagggat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt acagttggtg
     8701 tcgtcaatca gttttctcaa gtgaaagcaa tcttgatttg aaatagcgaa aaagagtctt
     8761 ccccagattt gaaattaaaa aaagcttatg cggatcaaag cgatggaaga aatgtgatta
     8821 aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca tcggttgaaa
     8881 tctacaaaaa agaaacaaaa gagccttacc tttcaataaa aaaagaaaaa atagatgtag
     8941 cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta gctccgggac
     9001 attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc cataccgatt
     9061 ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat ttaaaaaatc
     9121 agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt attggcttat
     9181 ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa caattgcatt
     9241 aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg ctgagaataa
     9301 gagctcagcc actacagaag gaacgatttc tgtgaccaaa ccagatgctg caaatcctga
     9361 agacgttgtt ccagttcctc ctgttgatcc aaatgatcct gatactcctt ttacacctga
     9421 cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag atcttgattt
     9481 tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa gcggaacgga
     9541 cagtactgga gcacaaacac aagtacagaa tttcgtacaa gtaacggata aaagcggaaa
     9601 ttatgccggt tggaaattat cagtcaaacg aacagaattt attggtacaa catctgatac
     9661 gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa atagcgcaac
     9721 taatagcagt ggaatgccat ctaccgtttc tggaactggc gctgaaggaa tcgaaatccc
     9781 agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg gcacatggat
     9841 ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat ttgttcctgt
     9901 atccaattat gctgtagaca actacgtttc aacattcaca tggtcgttaa cagatgctcc
     9961 tacagaagag gaagcgtaaa atcatatcaa cgacttcagg gagtggagcc gattcaaggc
    10021 ccactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact cgaagcagtt
    10081 ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc aacgagtaag
    10141 gagtcaccgt tgaaaaattc taacacataa tgagcagaat caattttctg caattgcgat
    10201 aagttgatac agtaagattt taaaagtaaa aagtaagcat acttttctaa ctctgtcttc
    10261 aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac cataacttgg
    10321 tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag gacgagactt
    10381 ccaccttttt gatcaataaa gattttatcg acatttacga tctgctgttc tttccaattg
    10441 atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct gatatatcca
    10501 atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc ggtgaaaaaa
    10561 acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat acctgtgagt
    10621 gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt gcccaactgg
    10681 atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat atgatgattg
    10741 accaattctc tgatgatcca ttcgctgtga aactggttat cttctatgat aaaatacttt
    10801 ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa caatggagga
    10861 ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta gatttaggta
    10921 tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggttgt atagctttga
    10981 tggtggtgac acttttactt cctatcccta aagtgagctt tccttaccta attggcttat
    11041 tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt ttgatcagta
    11101 atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg atggattatt
    11161 taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca tttattttct
    11221 atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt tttacttatc
    11281 caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg atttacttgg
    11341 tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt ttgtcgatag
    11401 tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa gtaaaagaaa
    11461 gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac catcatctag
    11521 aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact cttcaggaaa
    11581 ataaaaaaga aacgttggaa tttttagaaa tattagaaga tcattctgca gctatttttg
    11641 aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg ggtgtctttt
    11701 taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt attcctgtcg
    11761 aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct atttttattt
    11821 ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat ctgctggaag
    11881 aaaaagaggg atcacgtttt agtatagcga acaaggcaaa tccaacgaat ttgacgctat
    11941 cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt ctatacagtg
    12001 cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt tcaaaacaga
    12061 atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca gcctactgac
    12121 ttactgtcaa aggataagtt tgaggaaagt ctacacggag atagagacaa caacacagca
    12181 tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt ttgctatgta
    12241 gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta tacttcatga
    12301 tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct aactgctttg
    12361 cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga agcaagcaaa
    12421 tccctacgat cacaaaaaaa atgctgaccc agcagttaag agagttagaa caagatgaaa
    12481 gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt agagtactcg ctcaccgaag
    12541 aaggaaaatc attaagagag attctagtag cgttgtctgt ttgggaagaa gaacgtatcg
    12601 aaaaacaaaa agcctcaagg gaagaagaca cgctactgca tacacatggt ggctacctaa
    12661 attattgatt ttctatgaca tttattttaa ataaaagaaa acaggagttt ttttaaaagc
    12721 atcatctatt ttttatgtaa aaaaatggta tgattaatca agtaagaagt gattggaaga
    12781 aggcaagcaa atgaatagta tggaaaaagt caaccagttt agagatgaac gtaattggcg
    12841 acaatttcat aacgaaaaag atttggctat ctctatttct ttggaagcat ctgaattgtt
    12901 agaacttttt caatggaaaa cgccggaaga agtaaaagaa acacaattag aacgaatcaa
    12961 agaagaactg gcagatgtat tgatctattc atacatgatg gcagacaacc ttcacctgga
    13021 tctagatgag atcattgaag aaaaattgat caaaaataat ttgaaatatc ctgtagaaaa
    13081 aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa tgtgtcattc ggctgttgga
    13141 aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa aaaaaaggaa
    13201 aagcaaaaag atggctagtc aatatcctct tgttcctctt gttattagta ggactagctt
    13261 tgattttcaa tgaacagatc aaggattatt ttgtcagaga gacaggagat aaatatgcta
    13321 tagcgaatgt aacaaaagaa gatctgaaaa agaataacga taaagatgtc agttttgact
    13381 ttgatgcggt agaaccaatg acgacagaag gggtcatgag gtcgcaaatg agaggaacag
    13441 atctaccagt gattgctagt atcgcagtcc cttcagtttc aatcaattta ccggttttca
    13501 aaggcttgga taatacgtca ctactttacg gagcaggaac attatctcca gatcaagaaa
    13561 tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa ttgctgttta
    13621 caccgctaga aaatttagag atgggtgcca agatctattt gacagattta gaaaatgtgt
    13681 atacatacaa aacatttttt aaagaaaaag ttgctcctac agatacccaa ttactgaatg
    13741 aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg cgacatggac gccgtcacgc
    13801 gactagttgt acaaggagaa ctggaaagtg ttacatcgat aaaagatgcg acagatgata
    13861 tgagatctgc tttcaatcta gagacaaaaa cattttagga tactgggaca taagtcgtta
    13921 cagattaaaa acagccaaag gatatgaatc gataaggaaa aaccaatttg ctgaaaaaaa
    13981 gagaaacgca aagaggtgca tctctgattt ggttgttttc tagatagact tgtgttccag
    14041 tttttttatt ttttatatga aaaggtttgc ataaataaaa attcgatggt atgatgattt
    14101 tgaataagaa atggattgtt actgtacggc aggcaaaacc agaattcagc taagaaacag
    14161 taggcgaatt ctaggttttt ttatttttct tgagagggcg aagcaaaatg aaaattgata
    14221 aaattt
//
LOCUS       DACDVT010000060        13829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDVT010000060 DACDVT010000000
VERSION     DACDVT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.061"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(183..2180)
                     /gene="tkt"
                     /locus_tag="IWL52_001934"
     CDS             complement(183..2180)
                     /gene="tkt"
                     /locus_tag="IWL52_001934"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9778474.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2282..2533)
                     /locus_tag="IWL52_001935"
     CDS             complement(2282..2533)
                     /locus_tag="IWL52_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9778475.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2680..3303
                     /gene="lexA"
                     /locus_tag="IWL52_001936"
     CDS             2680..3303
                     /gene="lexA"
                     /locus_tag="IWL52_001936"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9778476.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3483..3701
                     /locus_tag="IWL52_001937"
     CDS             3483..3701
                     /locus_tag="IWL52_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778477.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3990..4502)
                     /locus_tag="IWL52_001938"
     CDS             complement(3990..4502)
                     /locus_tag="IWL52_001938"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9778478.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4516..6813)
                     /gene="recJ"
                     /locus_tag="IWL52_001939"
     CDS             complement(4516..6813)
                     /gene="recJ"
                     /locus_tag="IWL52_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9778479.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRVGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6955..7389)
                     /locus_tag="IWL52_001940"
     CDS             complement(6955..7389)
                     /locus_tag="IWL52_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9778480.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7399..8187)
                     /locus_tag="IWL52_001941"
     CDS             complement(7399..8187)
                     /locus_tag="IWL52_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9778481.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8184..9125)
                     /locus_tag="IWL52_001942"
     CDS             complement(8184..9125)
                     /locus_tag="IWL52_001942"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9778482.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9302..9889)
                     /locus_tag="IWL52_001943"
     CDS             complement(9302..9889)
                     /locus_tag="IWL52_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9778483.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10110..11423)
                     /gene="obgE"
                     /locus_tag="IWL52_001944"
     CDS             complement(10110..11423)
                     /gene="obgE"
                     /locus_tag="IWL52_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9778484.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11711..12721
                     /gene="gap"
                     /locus_tag="IWL52_001945"
     CDS             11711..12721
                     /gene="gap"
                     /locus_tag="IWL52_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9778485.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12806..13741)
                     /locus_tag="IWL52_001946"
     CDS             complement(12806..13741)
                     /locus_tag="IWL52_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9778486.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3815 a         3099 c         2436 g         4479 t
ORIGIN      
        1 cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat tctttgtttt
       61 gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc ttctaacaca
      121 taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac aattgcttgg
      181 atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc aaattcttct
      241 aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct
      301 gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac acgtttcttc
      361 actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa gaggtcaaag
      421 cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt ttgagctttc
      481 acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt ttctccttgt
      541 gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc ttttgtagaa
      601 ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc tgttgtctcc
      661 atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat cacttgaaca
      721 ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct
      781 ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg gattgctgct
      841 aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc gtagatacga
      901 gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc gcggacaccg
      961 aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc agccaccatt
     1021 gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat agctttcgat
     1081 aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc aacttcataa
     1141 gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc ttcaaattgt
     1201 tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca tgcatcttca
     1261 gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc ttctggaaca
     1321 gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc atcttcacca
     1381 agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc gatgactgtt
     1441 tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat cgctttagag
     1501 attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata tgcttcatag
     1561 cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga aatatcgttt
     1621 gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga acttgcttct
     1681 tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata gtgatccatc
     1741 acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat tgccataccg
     1801 acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc atctgtatga
     1861 tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt caaatcatcg
     1921 attgtcactt gatagccagc taaatgaagc aagctataca acattgctga cccatgtcct
     1981 gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg atttactttc
     2041 aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa tccaggatgt
     2101 cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt aacgccaagt
     2161 tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt ttacctttat
     2221 ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc agtatttttt
     2281 actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct tttaattttt
     2341 ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata tgatgacgca
     2401 taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct tgttcttcgg
     2461 cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc tctatttttt
     2521 caggtgacag catgctcagc cctcctttta ctctccttca tattatataa aaactgaaaa
     2581 aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc gctttttata
     2641 tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca aactgaatca
     2701 agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg atacccgccg
     2761 actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt tcacggtcat
     2821 cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc tcgtgcaatc
     2881 gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat tccagtactt
     2941 ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga cttctttcca
     3001 atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat tcgtggagat
     3061 agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa gcaagaaact
     3121 gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac ttgcaaacga
     3181 ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct cgaaccaatc
     3241 atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag tcatatctat
     3301 taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg gtctcaaact
     3361 cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt tacaaaccga
     3421 acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag aggtgagaat
     3481 tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta ggtattatta
     3541 ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt actttgtggt
     3601 tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt catgaagatc
     3661 atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg actgctaatt
     3721 attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt cacatccttt
     3781 ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat tgcggaaaaa
     3841 gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa atagcgaccc
     3901 tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct tctttaagaa
     3961 tatcatcaag aatagcttct tttttctgct taatattcca ttaatgtaat gatgtcatat
     4021 cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc gcagcctacg
     4081 actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc tccagtagct
     4141 agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt atgcaatgtc
     4201 aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg aggtaacttc
     4261 ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg acatcctacg
     4321 atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt tgcataattt
     4381 acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg ggaaatatct
     4441 cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc ttttaaatcc
     4501 actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat cgctcaaaag
     4561 caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc tctcagtcaa
     4621 aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg taacaaattt
     4681 caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac gcaaatattg
     4741 agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga tcaatttaaa
     4801 caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat caggtgaaat
     4861 ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca gatccgctaa
     4921 atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa ttattgcctt
     4981 tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag gcacgaattt
     5041 caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc tctgttcacg
     5101 gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat cagataccat
     5161 cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta ccacatctac
     5221 ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg catcaagctg
     5281 actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga ttcttttgat
     5341 attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg
     5401 cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca gcaatacttc
     5461 atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta tatattggtt
     5521 catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga ctgctgcatg
     5581 atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa aaagattcaa
     5641 ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag taaggacaag
     5701 cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa cgccttcatg
     5761 ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg ctaatgcttc
     5821 ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca atttttttgc
     5881 ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag gacgtggatc
     5941 gcccacccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc
     6001 tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta tccgttctga
     6061 atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag tcaatgaaac
     6121 taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg gttcttccaa
     6181 taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa atggatagtg
     6241 ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct caggtagttc
     6301 gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta ccgcatcatt
     6361 tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga tcttttcttg
     6421 gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga atacttctac
     6481 ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga tcccatctgc
     6541 gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt ggattcggct
     6601 gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt cctttggatg
     6661 taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta acttaccgac
     6721 aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag gctctgtctc
     6781 gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt agttattata
     6841 gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca gatccgctag
     6901 acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc cctattagtc
     6961 aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg ctttcaattg
     7021 actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca actccgcacg
     7081 ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct tttgaagttc
     7141 tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt gctgccatag
     7201 tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac cgatgatcac
     7261 taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat ttttattgtt
     7321 caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca cattgctttg
     7381 ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat aatcaccaat
     7441 cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt gattgatttc
     7501 ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag gttctaaaac
     7561 gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga agaaatttgt
     7621 ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca attctaatcg
     7681 caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg ttgattttgc
     7741 agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc ttctttctgc
     7801 catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga cttcaaacat
     7861 atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt
     7921 gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata attcgtccac
     7981 gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc tcaagcgtct
     8041 gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta taacgattgc
     8101 tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac tacctgttac
     8161 taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg
     8221 ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc
     8281 tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag ccgttttgcc
     8341 tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc
     8401 attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct attttccgca
     8461 agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt
     8521 ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag ttggatcgtt
     8581 tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc ttttagacga
     8641 tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa actgtcgatc
     8701 ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat cggggctgtt
     8761 tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat acctaaagag
     8821 acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt atccccacct
     8881 tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt
     8941 gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc
     9001 gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag tttcaatgct
     9061 atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa aaattgtaat
     9121 tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa aagaagggat
     9181 aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt
     9241 ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct gctgtttttt
     9301 attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc
     9361 aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca tgcacatctt
     9421 tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc attaccaatt
     9481 gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta gctccttcac
     9541 cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct ttggcatctt
     9601 gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc ggaccaccta
     9661 atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta ccggtaaagt
     9721 cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg acaactgcca
     9781 tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa aatgcctgat
     9841 atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt aaaaacgccc
     9901 ctttcatacc ttcttgttat ttattttacc tttaattgta aaataagaaa agaaaagcac
     9961 tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc tttttccgaa
    10021 taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc tgaatctata
    10081 ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc aaactcaccg
    10141 atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc cacacccata
    10201 ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt cgtcatttgg
    10261 aataattttt cgatcttctc accagataag atccacgttg catctgaatc acgatcgatt
    10321 tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc cagttcttct
    10381 tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt caataacgcg
    10441 tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc agcaaattca
    10501 tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg tgcatccggc
    10561 atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa gttataagta
    10621 gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc catcccactc
    10681 atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc
    10741 agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc ggcaaaactt
    10801 ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa atgataagcg
    10861 ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc gacagatgga
    10921 aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt tcgctcttgt
    10981 cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc aaaacggata
    11041 ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt ttcaattaaa
    11101 tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg tactttaaca
    11161 taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc acctggctgc
    11221 gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc
    11281 aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc aggaacgtat
    11341 ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac atcgatcgtt
    11401 acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat ttatttgact
    11461 gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat ctgattttgt
    11521 ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg gtaaggtttc
    11581 caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc gtagcagaat
    11641 aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta ataaaggagg
    11701 aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg gccgtctggc
    11761 tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca acgacttaac
    11821 aagtccaact atgttagcac aattattgca atttgactct actcatggta cttatcctgg
    11881 cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc gtgtttatgc
    11941 agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata tcgtactaga
    12001 atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa
    12061 acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata acgtcaatga
    12121 tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa caaactgttt
    12181 agcacctatg gcttatttct taaataatga atttgggatc gaagttggta caatgacaac
    12241 agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag gcggtaattt
    12301 gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg ctgcgaaagc
    12361 aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac aacgtgttcc
    12421 agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag taactgctga
    12481 tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg ggtatgatga
    12541 ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct ttgacccaac
    12601 gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg catggtatga
    12661 caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg ctaacttgta
    12721 atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag gcttctttct
    12781 tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat tgttggacta
    12841 cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct ccttttacaa
    12901 catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt tcaaatggta
    12961 caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc ttcaattgtt
    13021 ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact agactcgtta
    13081 cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa gcaagctcat
    13141 catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct ggtagttttt
    13201 ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga acgatttctt
    13261 gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct ttacgctctg
    13321 gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca tcaggaacaa
    13381 ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc
    13441 ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca ggaagataaa
    13501 tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg ttttcttttg
    13561 aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca ggtgtatctt
    13621 cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa ttccctgcaa
    13681 acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc caaagtttca
    13741 taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata atcaaagaat
    13801 ctctactatg caatgttcat ttttataac
//
LOCUS       DACDVT010000061        13720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDVT010000061 DACDVT010000000
VERSION     DACDVT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.062"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            54..506
                     /locus_tag="IWL52_001947"
     CDS             54..506
                     /locus_tag="IWL52_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778487.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IWL52_001948"
     CDS             520..1209
                     /locus_tag="IWL52_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778488.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="IWL52_001949"
     CDS             1334..3685
                     /locus_tag="IWL52_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9778489.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="IWL52_001950"
     CDS             3765..4160
                     /locus_tag="IWL52_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778490.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="IWL52_001951"
     CDS             4485..5102
                     /locus_tag="IWL52_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9778491.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="IWL52_001952"
     CDS             5143..5442
                     /locus_tag="IWL52_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778492.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="IWL52_001953"
     CDS             5476..5643
                     /locus_tag="IWL52_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9778493.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6293)
                     /locus_tag="IWL52_001954"
     CDS             complement(5688..6293)
                     /locus_tag="IWL52_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9778494.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6418..9405
                     /locus_tag="IWL52_001955"
     CDS             6418..9405
                     /locus_tag="IWL52_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP9778495.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9554..9763
                     /locus_tag="IWL52_001956"
                     /pseudo
     CDS             9554..9763
                     /locus_tag="IWL52_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9898..10074
                     /locus_tag="IWL52_001957"
                     /pseudo
     CDS             9898..10074
                     /locus_tag="IWL52_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            10056..10412
                     /locus_tag="IWL52_001958"
     CDS             10056..10412
                     /locus_tag="IWL52_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778496.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
     gene            complement(10926..11093)
                     /locus_tag="IWL52_001959"
     CDS             complement(10926..11093)
                     /locus_tag="IWL52_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9778497.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(11181..13121)
                     /gene="tet(S)"
                     /locus_tag="IWL52_001960"
     CDS             complement(11181..13121)
                     /gene="tet(S)"
                     /locus_tag="IWL52_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP9778498.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            complement(13122..13208)
                     /locus_tag="IWL52_001961"
                     /pseudo
     CDS             complement(13122..13208)
                     /locus_tag="IWL52_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT         4618 a         2122 c         2496 g         4484 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat tgccaattgt
     6241 cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc cataaaaaac
     6301 tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta ctgctatcat
     6361 agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag ggtttaaatg
     6421 gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc tagtacagaa
     6481 caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga gactatacga
     6541 cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt tttaggaact
     6601 tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc aactcaacta
     6661 aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta tcagcacaca
     6721 aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat agaaacatac
     6781 ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga tatgttgttt
     6841 gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat tactactttt
     6901 tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg acaattgtct
     6961 ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt aattggaact
     7021 ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc tgacgatagt
     7081 cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt ctctactgaa
     7141 aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa ttatgcattt
     7201 aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc gttgttagtg
     7261 gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc actatctaat
     7321 ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg tcttcgtacc
     7381 ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt agtaatgtcc
     7441 agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa atattctgag
     7501 gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga tcgtgaacca
     7561 atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat tccggatatt
     7621 ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga actctggacc
     7681 ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc tattaaacat
     7741 actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac taataaatgt
     7801 gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga tatttatatt
     7861 cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa aacttgggtt
     7921 caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa tgaaatgtct
     7981 ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg ttggcctact
     8041 tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact taaaaagaca
     8101 tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact tcctaaaatt
     8161 gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca atcatttaac
     8221 cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt agcagttctt
     8281 ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt tgattcttta
     8341 aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac actttcagga
     8401 gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat atggggtgat
     8461 gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc cctatattct
     8521 agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa ttttgtctct
     8581 gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga ttcgctttac
     8641 cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat catgactgat
     8701 acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt tcaatttagt
     8761 ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa cgctcactac
     8821 ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta tacccattgg
     8881 gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc aacagaatta
     8941 acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat aactgagtat
     9001 ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat atatgcacgg
     9061 caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg taatattttt
     9121 tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga gcagctcaat
     9181 gcattaggat tagttactaa tgccatcata tattggaact cactgtattt agaaaaagta
     9241 tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg caggcaatct
     9301 ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata cgaagctgaa
     9361 ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg ttcgggtttt
     9421 gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat agaaaaagag
     9481 aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg tgctaccgca
     9541 ctatttcgtt tctatgttcc atgtacccct ttatcaagaa gcgcaaacac gccaaattgc
     9601 cgattggttg atcggcatga acgcaagccc tttatatacc ttgaatttac aacaaaaagg
     9661 cgtacaaggt acattttcat taggacgtgt acaaacaccg actttatatc tcatttatca
     9721 acgccaggaa gcaatagaaa actttaaaaa agaacctttt ttcctgaata attcatagtt
     9781 ttactaatag tctttcctaa cattgattta atagtcttta aagggttctg ttgcaaagtt
     9841 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
     9901 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggctactat
     9961 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac
    10021 gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact ctatcaaatt
    10081 ttgatttcaa accattgggt cttgcgatta aagaagcccg tcttagtaaa ggattgactc
    10141 gtgagcaggt aggatcaatg atccagattg cgccacgcta tttgacgaac attgaaaata
    10201 aaggacaaca gccaagtttt catgttgtat atgatttagt gactttgcta aatatttctt
    10261 tggatggatt tattttaggg gaagaaaaca gtcataaaag ctccaaacgt cttcaagtag
    10321 aagcacagtt ggattgtctg gatgaagatg aattagtgac gattgggaaa gtgattcaag
    10381 caatttacgg agaaaaatgt aaatttaact agttcaataa tacacaatgg attaattaat
    10441 actatcagga aagattgatt acaaagatgt aatgaatttt ttctgatttt tgttaatcat
    10501 aaatagaaaa tgaatttatc tttgttacac ttaggaacta gagcgaatga taaatagttc
    10561 tttggttact cttatcgctg caaagtacag ttcatttttt gttgttggga aaattttctg
    10621 gatttattgc atatcacttc atccgactaa aaatttttga ttcccatagc gaagttcgtt
    10681 tttgttcctt aggaaaaact tgactgatat actagttttg aaatgacgag taactctgta
    10741 cttcatcaat aatcaagtat tgaattttaa ttgtatactt tttatgcttg agggatgtct
    10801 actgtaatac agctactgca aaaaaaatca gtacacttat tttcttataa aggatatcaa
    10861 gaactaagct caatggtatg catagaaaag aaaaagccga taaaatgaga ttttgatatc
    10921 tcgaattatc ggctctgcgt ctttgcgtct ggctctgtaa ttacagttat tctaaacttc
    10981 gttatttcaa ttaaaatttc ttgtctgcat ttaggacaaa atagagggaa attttttaat
    11041 tcagtatctt ccctcatttt taaacgggtc ttattgccac atatagggca cagtatccac
    11101 ctagtattta caatatctat atcctcctgt tctaattaac aactcaatat tcattttaaa
    11161 aatttctctt tagttcacta tcaatgtaag ttgattttat taaacatatg ccttactttg
    11221 tctattctat tatttggacg acgtggttgg aaagcagact taatgttagt aacctgataa
    11281 ccttttaact ctgttaaaca gacactgcgt ccatttgtaa agaaagttaa actgtttcga
    11341 tactcttgaa tacaacgggc tggaatttca ccaatgagaa tgacctcgtt accttttaac
    11401 ttagtattta aaatatttgc acaatatttg gaagcatcat tatatgctct cgaaagatat
    11461 tcttgtggta cataaatttc gaagctaaga tatggctcta ataactctgt accactcttt
    11521 ctaaaagcct gctctagtac aataggcgca agcattcgga aatctgctgg cgtactgaca
    11581 gggctgtaat atagaccata cttaaaacag atcttacagt ctgttaattt ccaaccgtac
    11641 aatccttgtt cacacccata gcgtatacct tccataactg cattttgaaa tgattgattt
    11701 aaataaccta gagaaaccag gctctcatac tgaatgccac tacccaaagg aagtggtgtt
    11761 acagataaac caatagaagc ccagaaagga tttggaggta cttcgatatc aatggtaaat
    11821 tcagattttt ttaatggtct ttccatataa atgacagttg gttttcttgt ttctatctca
    11881 atatgatatt tttcttgaat cagagtacaa gttacctcca tttggacctc acctaaaaaa
    11941 gatagcacaa tttcgtgagt tactgtatct acataatatt gtagaagggg atcactatcg
    12001 gatatttcaa aaagtgcatc tagtaacttt tctctttgta ctgatttaca tggttcaatt
    12061 gttgtttgta acataggaag aggattctca agaatttctc tatgtggtaa tcttttttta
    12121 tctccaagta cgttatttag ttttaaaagc tcatttttta aaataataat ctcaccaggc
    12181 tctgccttat ctatctggcg taattctcca tttattgaag tatacatttc tgtaactttt
    12241 attttttcct tttctgatat attgactgag tctcgcaaat gtagcgttcc actataaaga
    12301 cgtacataga caagccgttg accatcatct gaatattcta ctttaaaaac atttccacaa
    12361 agtttatctg aattgagttg tgtgggtgaa aataatttgc tagtaattac ctctataagt
    12421 tgtttaattc caatattatt ctttgcactt ccgtgataaa caggatataa ggagcaactt
    12481 tgaattcttt cgttttcctc cttttcaagt tctgcaatat tcaatgtttt cccaatggta
    12541 tatttttcta ataaataatc atttcccaca attactgtct cccattgttc tggttcagta
    12601 taatctatta cataaggttt caaatttaga ttcacagtct gcttaattat gatgtcgtca
    12661 gaaagtttgt ccttaatatc ttgataaaca tctggcaaat ttattccatt ttgatcaatt
    12721 ttgttaataa aaaatattat aggtatgttc atctttctaa gtgcatggaa tagtatacga
    12781 gtttgtgact gtactccatc ttttgcagag attagcaaaa tagctccatc caaaacagat
    12841 aatgaacggt atacatctgc caaaaaatcc atgtgtccag gagtatctac aatatttact
    12901 ttaacatttt cccgttgaaa agatgttatt gcggtctgaa tagtaatacc tctctggcgt
    12961 tccaaaaaca tagtatccgt tttcgttgta ccgctatcta cacttcctaa ctctttaatt
    13021 gctccgctac tgtatagtaa gctttctgtc aaagtagttt ttcctgcatc aacatgtgct
    13081 aagataccga tattaataat tttcaatttt atttcctcca ttcaaaagct caaaaagcag
    13141 aaaaaatccc agtgataaat actcttatca ctgggatttt aatacaaaaa tagctgtata
    13201 ctacacactg atagccacag atgctttatg ataaaatgat aaaagtattc ttaaactggg
    13261 cacaaaaaac cagtccttat tttaaataag gatgcttaat taattattcc cgctatctaa
    13321 ttgacagttt atttaagaat accttgccgc atttttatta tctccttttg aatgttatct
    13381 taattctatc atatgataga atgcttgtaa agccaaccat attttttgaa cgaaagggaa
    13441 attaatgaga aattaatgga agttagtgta gtagttgaag ataactcttc agaatggcta
    13501 tcaaaattca gcgatttaat aacgatttac aagttcataa gggattcttt attcatccag
    13561 ctattatatt acttttcagg tcaataggac aactgtccac aacctttcta caaatatatt
    13621 acgtataatt tttgaaatat atactgctta acaaaatatt ttgcagtggt tccaatagtt
    13681 ttgaattgtt aatattaagt ttgacttgga aaatatcatt
//
LOCUS       DACDVT010000062        13679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDVT010000062 DACDVT010000000
VERSION     DACDVT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.063"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..903)
                     /locus_tag="IWL52_001962"
     CDS             complement(<1..903)
                     /locus_tag="IWL52_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9778499.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSES"
     regulatory      complement(927..971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1186..2154
                     /locus_tag="IWL52_001963"
     CDS             1186..2154
                     /locus_tag="IWL52_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9778500.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2267..2545
                     /locus_tag="IWL52_001964"
     CDS             2267..2545
                     /locus_tag="IWL52_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9778501.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2663..2899
                     /gene="secG"
                     /locus_tag="IWL52_001965"
     CDS             2663..2899
                     /gene="secG"
                     /locus_tag="IWL52_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9778502.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3011..3757
                     /locus_tag="IWL52_001966"
     CDS             3011..3757
                     /locus_tag="IWL52_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9778503.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3760..6117
                     /gene="rnr"
                     /locus_tag="IWL52_001967"
     CDS             3760..6117
                     /gene="rnr"
                     /locus_tag="IWL52_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9778504.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6139..6603
                     /gene="smpB"
                     /locus_tag="IWL52_001968"
     CDS             6139..6603
                     /gene="smpB"
                     /locus_tag="IWL52_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9778505.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6905..7624
                     /gene="atpB"
                     /locus_tag="IWL52_001969"
     CDS             6905..7624
                     /gene="atpB"
                     /locus_tag="IWL52_001969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9778506.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7677..7892
                     /gene="atpE"
                     /locus_tag="IWL52_001970"
     CDS             7677..7892
                     /gene="atpE"
                     /locus_tag="IWL52_001970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9778507.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7983..8507
                     /gene="atpF"
                     /locus_tag="IWL52_001971"
     CDS             7983..8507
                     /gene="atpF"
                     /locus_tag="IWL52_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9778508.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8494..9036
                     /locus_tag="IWL52_001972"
     CDS             8494..9036
                     /locus_tag="IWL52_001972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9778509.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9059..10615
                     /locus_tag="IWL52_001973"
     CDS             9059..10615
                     /locus_tag="IWL52_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9778510.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10629..11531
                     /locus_tag="IWL52_001974"
     CDS             10629..11531
                     /locus_tag="IWL52_001974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9778511.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11554..12960
                     /gene="atpD"
                     /locus_tag="IWL52_001975"
     CDS             11554..12960
                     /gene="atpD"
                     /locus_tag="IWL52_001975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9778512.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12974..13396
                     /locus_tag="IWL52_001976"
     CDS             12974..13396
                     /locus_tag="IWL52_001976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9778513.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4522 a         2448 c         2980 g         3729 t
ORIGIN      
        1 actttcggaa aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg
       61 ccaaaattca taacgatcaa ccaatgtttg tccacgactt attccctgag tttccacagc
      121 ggtgaagctt ttgaaacctt cacagatggc cccctcgatg aaataagcaa cagctaacgg
      181 atcattgatg acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg
      241 agtgatcgct tttaaatact ctccctcttc aggattcatt tgacagcagt attcgaggat
      301 agtcggtgtc aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt
      361 gagattctcg aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac
      421 tggcgaacag ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg
      481 tttcccaata tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc
      541 aatgactgat gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag
      601 aggttctgcc tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc
      661 atgagtgtct tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac
      721 aggaatgtct aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc
      781 tgcagggaca ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac
      841 agctaatgat aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg
      901 cattatttcc aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga
      961 accagtccaa gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg
     1021 cgaatgtaaa tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca
     1081 atgaaaaaga aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt
     1141 ggataataga tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat
     1201 ttcattctta ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca
     1261 aagaaattag taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat
     1321 cgtttgatgg accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa
     1381 gagtgtgaaa gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg
     1441 aaactagctg gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta
     1501 catgattacc gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag
     1561 aaaggattca acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc
     1621 atcggaatgg gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat
     1681 gaacagccga atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg
     1741 atggcaagtg gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat
     1801 gtttctgttt atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa
     1861 atggtcatga gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa
     1921 gcatcagcta cgagacagct tggcagcaac catctgcctt tattagtcat ccatggtgaa
     1981 agagatcatt ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat
     2041 aaagcactgc tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa
     2101 acctattgga cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttaattttct
     2161 cttctttttt gtaagtaatt cgaggataaa taattgttta tagatctgga gactgctaaa
     2221 ataaggtagg aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga
     2281 agaaaaaaat cgtcttgctt tattcaatga tgagcaacaa gagattggag aaatgacatg
     2341 gtcagatgcg ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg
     2401 tggtcaaaag ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg
     2461 gaaaaagatc attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata
     2521 tcaagacgtc ttacgtcagt cataagaata acagctgaaa gttttgaact ttcagctgtt
     2581 attccattta tatagagttg tcacggactt ttatctatga taaagttaaa gagacgatat
     2641 cttttgggaa ggaggctgcg atatgtataa tttaattttg ggaatcgtga tcgttttatc
     2701 gattgttatg gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc
     2761 atttaccggc ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt
     2821 catgcagcga tcaactgcag ttatgggagc tatctggatg atcttattat ttgtcctagc
     2881 atatttatct tcgaagtagt tgaataagcc tctcgcaaaa cggcgggcgg ctttttttat
     2941 tgcaaagaga acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga
     3001 ggtgagacga gtgagacaat taccaaaacc cctttatgca aaacatggaa agcgagcggt
     3061 tttgctgctt cacgcttact caggaagtcc aaacgatgtt cgaatgctcg cacgattttt
     3121 ggaaaaagca gattatacag tttatgcgcc gctttttaaa ggacacggaa caatggagcc
     3181 atatgatatc ttgcaagaaa aagcagaaag ctggtgggca gacactaaaa aagccatcca
     3241 ttttttacag tctgagcagt tttctgatat tgccgtatta ggattatcaa tgggcggcat
     3301 ctttgctgta cgagcactag aagaagaatc agtgattggc ggcggttttt tctgttcgcc
     3361 tttgtctcct gtaaaaacaa acgtgcctga aaactttgaa aaatacgtac gtcaagtgct
     3421 gaagacagca ggtaaatcag agaaagaaat caatgaaaaa gcagttgctt accgctcgtt
     3481 agctgaacaa caattaatgg atatacaaga tcaggcagca atcgtagaaa gtaggttgtc
     3541 agatatccaa cagccgatat ttttagctca agctgggaaa gatgaaatga tcgaccctaa
     3601 tggcgtattt gaaactgcac gcaagttgag ccgtcagcga gtgacacttc aatggtatcc
     3661 tgaaagtggt catgtgatta cagttggtac agcaagacgc gaattagaaa aagacgtctt
     3721 ggaattttta gaaaaattac cttggaatga ggaataaata tgacaaaaga aacgataaaa
     3781 gagaaaattc tctttttcat ggaaagcagt cgcaaacgca gtttttctat ggaagaaatc
     3841 gctgaaggtt tagggttcca aaaaagcgat gattttaaat tactagtaca aacgattgct
     3901 caaatggaaa gagaacagag tatagttttc aataaaaaag gaaaagtgaa acttccttta
     3961 aaaccaatct tgatcgaagg aacatttcga gcaaatgaac gaggatttgg atttgtaacg
     4021 attgatcccg aagaagatga catctatatc ccaaaagaag cgactggttt tgcaatggat
     4081 ggagacaccg tagcaatcga tatcatgaag cgagcaaata cagatatgga tcgcggagca
     4141 gaggggaaag tagtcgagat tcgagaccgg gcgactactc agttagcagg ggaattcgtt
     4201 gcttattcag aagaagaaat acaagaaaca gatctttacg gcgttgttat cccgaaagat
     4261 agaaagctca accaatttaa agtatatgca gcagcagagg gagttcgtcc agttgacgga
     4321 agtattgtgc tgatcgaact gacccattac cctgaaaaag ggtatgcgac tagtttagaa
     4381 gggataatca agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcaatt
     4441 gtcgtggcta atggtatccc tacgaaattt cctgatgatg tattgtctga agcagatgaa
     4501 gttccagatc aaatcagcga aaaggatata actggacgta gagatttaag aaatcagctc
     4561 attgttacga ttgatggaga agatgcaaaa gatctagatg atgcagttac ggttaaaaaa
     4621 ttagataacg gtaattattt tcttggagta cacattgcag atgtttctta ctatgtgaca
     4681 caaggcagtc aattggacag agaagcatat gaaagaggga caagtgtata tttgactgat
     4741 cgagtgattc cgatgatccc gcaacgttta tcgaatggta tctgttcact gaatccccat
     4801 gttccaaggc tgaccatgag ttgcgaaatg gagattgatc caaatggaca agtagtctct
     4861 catgatattt tcccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat
     4921 caaatcttag aagatcaaga cgaagaaacg atgcaacgct atgctcagct cgtacctatg
     4981 ttccaagaaa tgggtgaatt gcatcgaatc cttgaaacga tgcgaatcaa acgtggcgcg
     5041 atttcttttg aggatcgcga agcgaaaatc cttgttgatg ctgaaggaca cccaaatgac
     5101 atccagctaa gaagcagagg aattggtgaa cgcttgatcg aatctttcat gcttgcggcc
     5161 aatgaaacgg tagctgaaca ttataataaa ttaaatctgc cgtttattta ccgaatccat
     5221 gaacagccaa aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt
     5281 ctggtccgag gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga
     5341 gtagaagaca aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa
     5401 gcacgctatt cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac
     5461 tttacatctc ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac
     5521 agtgaagata aaagtgaaag caatcaaagt tcgtgggctg cggaacttcc tgaaattgca
     5581 gatcatagtt ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg
     5641 aagaaagcag aattcatgat ggacaaagtg ggagaagaat tcgatgggat catcagctct
     5701 gttgtgaaat tcggcctgtt tatcgaactg ccgaatacag tagaaggatt gatccatatc
     5761 aatgaattga aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa
     5821 agaactggtc tgacttttaa aatcggacaa aaagtgcgcg taaaagtagt caaagctgat
     5881 ccagatgaac gagcagtgga ttttgaactc gtttccgcag aagaggtcac tgcgttggaa
     5941 cgtccaaaag cacgcaatgg aaaatctgga aaacgagaag gaaaacaagc aggcaaacgc
     6001 tcagaaaaaa atggcaagaa gaacaaacga tatagtgata gtaagcaaac gttcggtaag
     6061 aaaaaaggga aaaagccatt ttacaaagat gtcaaaaaga agaaaaagaa aaaataaaat
     6121 gagttggagg aaacagctat gccaaaaggc gagggaaaac taattgcaca aaacaagaaa
     6181 gctcgccatg attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacg
     6241 gagatcaagt cgatacgaaa cagccggatc aatctgaaag atggatttat tcgcgtccgc
     6301 aacggggaag ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt
     6361 aatcatgatc cgttgcgcac gagaaagtta ttattacaca agaaacaaat cgttcgtctt
     6421 gaaaacgaat tgaaaaacac tggaatcact gttgttcctt taaaagtcta tattcgtaac
     6481 ggctatgcca aggtattgat tggtctggca aaagggaaaa aatcgtatga taaacgggaa
     6541 gatttgaaac gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga
     6601 taatttcatt atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt
     6661 gttagcattt aatctgataa aacatgatta tcacaggttt tttcttagat caggagcacc
     6721 gtgcatgggg aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg
     6781 aaaatgtttt gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg
     6841 gaaaaaacca gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa
     6901 tggattggat gaacgctcgc taacgtttca cattggacca atttggtttg atggtacagt
     6961 ttgtataatg gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag
     7021 aaatcttaag ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag
     7081 tcgaggaatc gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc
     7141 atttacattg tttttatttg tttttgttgc aaacaacata ggtttagtaa caaaaatcgt
     7201 cttacctaac gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac
     7261 tttggcattt ataatgatct tacttactca cttattcggg gtgaaaaagt taggcttcaa
     7321 aggatatttc gtaaattcgt tcttgaaacc ctatagtttt atgtttccga tgaaactgat
     7381 tgaagaattt acaaatttat taacacttgc tttacgtctt tatgggaata tttatgcggg
     7441 ggaagttttg ctaaccttga ttgcaaaaat gatgacaagc ttaggctggt tctcattacc
     7501 attagcaatt ccattagaga tggtctggat cgcgttctcc ttgtttattg ggagcatcca
     7561 agcatttgtc tttgtcactt tgtcaatggt ttatatgagc cataagattg aagtagaaga
     7621 ataatactcg gttaaaaaac aaactactat ttatctattt taggaggaaa acaattatga
     7681 attatatcgc agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg
     7741 gtcaagttat ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat
     7801 tccgtacaac aatgtttatc ggggtagctc tagttgaagc tgttcctatc ttaggggtag
     7861 ttatcgcctt gattctagtt tttgccgttt aattgaatca gtattaaggc agaaacaaca
     7921 tcgtttgtta ctgctttttt acatggaaac tagaaatact ccaaagaaag gaaggctcgc
     7981 ctatgctgaa tcaattggca attgcagaag ttggcaatgc gatgttaggt aatatcatcg
     8041 ttgtcagcgg ctcatttttg atactactgg ctctcttgaa gcactttgct tggggaccaa
     8101 tcagcgatat tttgaaaaaa cgtgaagaca agatcgccaa tgatttagat tctgcagaac
     8161 aatctcgcat caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc
     8221 gttctgatgc agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa
     8281 atattttgaa ggatgctcaa gaagaagcag ctcgtctaaa aagcaaagcc caagctgata
     8341 tcactgtaga acgtgattca gcgctgaact ctgtaaaaga cgacgttgca gaactctctc
     8401 ttcaaatcgc ggaaaaaatc ttgaacaaag aattatctcc agaaatgcat gaatcattaa
     8461 tcaatcaata cattgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga
     8521 agacgttacg ggaaagcttt atttgaactg gcgatcgatt caaatcaagc agaagaaatc
     8581 tatcaagatc tcgtaagttt acgtcaaatc tatgaacaag tacctggatt agggaatatg
     8641 ttaagtgatg ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgtcagc
     8701 ggttatgatg ggatcgttaa aaacttttta gaagttgttt atgagtataa ccgaatggat
     8761 gaccttttgt tcatgatcga agaatatgaa cgtcgatatg atgaacatca agggttacta
     8821 ttgggaagtg tcacaacagc agtcccactt tcagacgaac aacgaataca attagaaaag
     8881 aatgttgcaa aaacaatgga ctatcaatca gttgaattaa aacaaatcgt tgattcttct
     8941 attattggtg gcgcgattgt tgaagccaac catcgagtaa tcgacggaag cattcgcacg
     9001 caattagaaa aaatgcgtaa tcaattaaac agatagtaca aagaggtagg tgaatcgaat
     9061 ggccatcaaa gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa
     9121 cgtgctttca gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc
     9181 acatggttta gaaaacgcga tgagtggtga gttgcttgaa ttttcaaacg gctcatacgg
     9241 aatggcgcaa aacttagagt caaatgatgt aggtatcatt atccttggcg atttcgagtc
     9301 cattcgtgaa ggagataaag taaaacgaac aggtaagatc atggaagttc cagttggaga
     9361 ggccttgatc ggtcgggtag tcaatccgct aggtcaacca atcgacggac taggtgaaat
     9421 cgttactgac aaagctcgtc cagtagaagc gatggcacca ggtgttatgc aacgtaaatc
     9481 tgttaacgaa ccaatgcaaa cagggctaaa agcgatcgac gcccttgtgc caatcggacg
     9541 cggacaacgt gaattagtca tcggtgaccg taaaacaggg aaaacttcta ttgcgatcga
     9601 tacgatcatc aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa
     9661 agattctaca gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac
     9721 aatcgttgtg aatgccggtg cgtctcaacc agcaccattg ctttatatcg caccatatgc
     9781 tggtactgca atgggtgaag aattcatgta caacggtaaa catgtattga tcatttttga
     9841 tgatctttca aaacaagctg ttgcttatcg tgaactttcc ttgctacttc gccgtccacc
     9901 aggtcgtgaa gcttatcctg gggatgtttt ctacttgcac tcacgtttat tagaacgtgc
     9961 agcaaaatta agtgatgatt taggtggcgg ttcaatgact gccttaccat ttgttgaaac
    10021 acaagctgga gatatctctg cttatatccc aacaaacgtt atttcgatca ctgatggaca
    10081 aatcttctta gaaagcgact tgttctacgc aggtactcgt ccagcggttg atgccggact
    10141 atctgtatct cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac
    10201 tttacgccta gacttagcaa gttaccgtga attagaagca tttacacaat tcggttctga
    10261 cttggatgca gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa
    10321 acaaaaattg catgcaccgc tagctgttga aaaacaagta gtgattttgt atgctttgac
    10381 acatggattc ttagatagta tccctgtaga cagtatttta gacttcgaac atgaattatt
    10441 cgaatatctg gataccaatc attcagatat ctttgaaaca atccgtacaa caaaagatct
    10501 tccagaagaa gaagcgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac
    10561 aaaaggaagt aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg
    10621 tgagatgaat gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa
    10681 cgagccaaat cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag
    10741 cttcttcaca gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga
    10801 ctgctactca gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca
    10861 gcatgctgct cagccgtgct gtgaagaaaa ctggttatat cgtcatcact gcagatggcg
    10921 gtttagtcgg tggatataac agctcgatat tgaaacaaac gatgtctatt ttagaagaag
    10981 accaccaatc acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct
    11041 ttaaagcacg cggtatcaat ctagcatatg agctgcgcaa tctatctgat cagccaagct
    11101 ttgatgaagt ccgcaaaatc gtaaatatgg caacaactat gttccaaaac gaagtattcg
    11161 atgaattata cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag
    11221 aaaaaatgct gccaatctca gacttagatc cagaagaagc aacgactttc gaacaagaat
    11281 atatttttga accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa
    11341 gtttgatcta cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag
    11401 caatgaaaac agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttata
    11461 accgtgcaag acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg
    11521 cactagaata gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta
    11581 atcggtcccg ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac
    11641 gcattagttg tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta
    11701 gaattagggg acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga
    11761 ggaatggaag tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta
    11821 ggtcgcgtat tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat
    11881 gcggaaagaa gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca
    11941 gaaattttgg aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg
    12001 aaagtcggac tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc
    12061 cataatatcg cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact
    12121 cgtgaaggta atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc
    12181 atggtgttcg gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt
    12241 ttgacgattg cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac
    12301 aacattttcc gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct
    12361 tctgcggttg gttatcaacc aacattggca acagaaatgg gtcaattaca agaacgtatc
    12421 acatctacga aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac
    12481 tacactgacc cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa
    12541 cgtaaattaa cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt
    12601 gctttagcac ctgaaatcgt gggggaagaa cattataaag tagcaacaga agtccaacac
    12661 gtattgcaac gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta
    12721 tccgatcaag aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa
    12781 aactttaacg tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa
    12841 acagtgaaag gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct
    12901 ttccgaagtg ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag
    12961 aaaggggtgc cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt
    13021 ctatgatcac catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt
    13081 gcctaaacat gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac
    13141 agattcagat acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga
    13201 taatgttgtt tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg
    13261 tgcggaacgt gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga
    13321 tacgaacgaa ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt
    13381 atctaagcac tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga
    13441 ggttatgaca gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc
    13501 aaatttttgt gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc
    13561 agaggttgta aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc
    13621 tcatattttg ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttatt
//
LOCUS       DACDVT010000063        13592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDVT010000063 DACDVT010000000
VERSION     DACDVT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.064"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..274)
                     /locus_tag="IWL52_001977"
     CDS             complement(<1..274)
                     /locus_tag="IWL52_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9778514.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKL"
     gene            complement(274..504)
                     /locus_tag="IWL52_001978"
     CDS             complement(274..504)
                     /locus_tag="IWL52_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9778515.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            791..1129
                     /locus_tag="IWL52_001979"
     CDS             791..1129
                     /locus_tag="IWL52_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778516.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1334..1573)
                     /locus_tag="IWL52_001980"
     CDS             complement(1334..1573)
                     /locus_tag="IWL52_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP9778517.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1549..1896)
                     /locus_tag="IWL52_001981"
     CDS             complement(1549..1896)
                     /locus_tag="IWL52_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9778518.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2628..3209)
                     /locus_tag="IWL52_001982"
     CDS             complement(2628..3209)
                     /locus_tag="IWL52_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9778519.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3392..4018)
                     /locus_tag="IWL52_001983"
     CDS             complement(3392..4018)
                     /locus_tag="IWL52_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9778520.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4040..5371)
                     /locus_tag="IWL52_001984"
     CDS             complement(4040..5371)
                     /locus_tag="IWL52_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9778521.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5526..5927)
                     /locus_tag="IWL52_001985"
     CDS             complement(5526..5927)
                     /locus_tag="IWL52_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9778522.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6013..6219)
                     /locus_tag="IWL52_001986"
     CDS             complement(6013..6219)
                     /locus_tag="IWL52_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778523.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6314..7816)
                     /locus_tag="IWL52_001987"
     CDS             complement(6314..7816)
                     /locus_tag="IWL52_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9778524.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7834..8289)
                     /gene="argR"
                     /locus_tag="IWL52_001988"
     CDS             complement(7834..8289)
                     /gene="argR"
                     /locus_tag="IWL52_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9778525.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8387..9004)
                     /locus_tag="IWL52_001989"
     CDS             complement(8387..9004)
                     /locus_tag="IWL52_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778526.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9225..10598
                     /locus_tag="IWL52_001990"
     CDS             9225..10598
                     /locus_tag="IWL52_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9778527.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10673..11785
                     /locus_tag="IWL52_001991"
     CDS             10673..11785
                     /locus_tag="IWL52_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9778528.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11857..13494)
                     /locus_tag="IWL52_001992"
     CDS             complement(11857..13494)
                     /locus_tag="IWL52_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9778529.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3702 a         2864 c         2394 g         4632 t
ORIGIN      
        1 tgagttttcg ttccttaaaa tcaagagatt ttaattgtgt aataattact tgtcctttta
       61 tatctagctc ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg
      121 gacatatgac cgaaaacatt gttttacggt taaattcata acgagagacg actagtcctg
      181 gtcgtctttt ttgaatttct tttcctacgg agggatcaaa atcaatccat acaatatcac
      241 cttttttagg aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt
      301 taaatcttcc cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc
      361 ttcaattttt ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg
      421 gttttctgaa ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg
      481 taatctagat tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac
      541 actaagtaac gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat
      601 atcttattgg atataaatag aaataaattg tagccagttc attttttctt taactaaaaa
      661 aataatccaa gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc
      721 ccccgctacc ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg
      781 ccatcctatc atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt
      841 tcaattggga aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga
      901 atgcccgctt caaatagaaa cattggttca acgaatttca actagacaag actttgctat
      961 tgtagaacgt gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca
     1021 tatcaatgtg gataaatggc aacctcttat ctataaattc agagaatata caactgccaa
     1081 ccagtcgtta actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc
     1141 gttaggtata aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt
     1201 agaaaggtcg actgtgaccc tatagatgca gtatatgcag cttatgagca tttcttctgt
     1261 tctctttgtt tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga
     1321 aaaatcgatt atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt
     1381 ttcgcgcaaa agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg
     1441 gcattcggtc atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt
     1501 ctgccatttt ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct
     1561 tatttgaaaa cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt
     1621 ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat
     1681 catcaattgt ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat
     1741 gttttcttcc tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc
     1801 taattctgct gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag
     1861 ctctgactct agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt
     1921 ctatcataca ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact
     1981 aaaaaagttg tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg
     2041 gtcacaagtc acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga
     2101 ctcggagtga aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct
     2161 cggtattaag ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa
     2221 tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca
     2281 tctgcggtaa taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc
     2341 ttattacttg atgcagggca gctttttcac aacctctcca ctaaacgatg ttcaatcagc
     2401 taaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt
     2461 ctctaatact caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt
     2521 agcttcttcg aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt
     2581 ccttcttatt tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt
     2641 tgcgaagtta acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc
     2701 tgtgtgactt tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga
     2761 cggtctgctt cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta
     2821 tacttgaccc attgattgac taattctagt agcaacagac ggtattcttc atccactgta
     2881 ctcaactcaa taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga
     2941 tcagcgaaaa tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt
     3001 aagatgtctt cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga
     3061 caaacttctt tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct
     3121 gctagaatat ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc
     3181 gcttccaaga tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc
     3241 tatattatac agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa
     3301 attatctgac acagccctca ctataaaact gttctcatat agctagatgt tcattgattc
     3361 aagaactgcg aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg
     3421 ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt
     3481 ggccaggagc accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt
     3541 cacgcatatg ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga
     3601 ttccattttt tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag
     3661 gagagctgat gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc
     3721 ctgaaccttc ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca
     3781 tcatatgttc tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag
     3841 gagaaataaa gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt
     3901 cgatcgtatt gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt
     3961 tcaagaactc gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg
     4021 tggattctcc attcgttatt tattttaata catcatttag agcttcactc attgtcggat
     4081 gagaatagat ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa
     4141 tcaagttgat gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag
     4201 tttcagggtc gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag
     4261 ggacaccgct tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt
     4321 tttctgttaa acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt
     4381 tccggttatt cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat
     4441 catctagaga gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt
     4501 tgtctgcatc tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa
     4561 catctgtatt ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa
     4621 cttttgacgc ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg
     4681 attcttggtc tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg
     4741 tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat
     4801 gggcatcaag aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc
     4861 caatatatcc agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt
     4921 gcgtagaatc aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt
     4981 cagaaccagt attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg
     5041 tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg
     5101 cctcatcgct gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc
     5161 gagcaacagc ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag
     5221 gaatacagcc gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta
     5281 aaaccgattc accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac
     5341 cgatcacaat attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga
     5401 ttggtacaaa aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt
     5461 caaaaaaaac cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta
     5521 ttcttctaaa caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg
     5581 cagaattcat gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata
     5641 tgcacttcta atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca
     5701 cgtgctttta ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc
     5761 ccattgaaac aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca
     5821 tggattcctg gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca
     5881 ttgcgcccgc catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt
     5941 tcgattgacc atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta
     6001 caaaaattct gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa
     6061 aaaaatacta ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc
     6121 tacataatct tttatatgat tgattttaag atacatatat accagaaagt tgaccccata
     6181 aaccaatact gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc
     6241 tccctaccag cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt
     6301 caaaaagact ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt
     6361 ttgctaattt tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata
     6421 ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt
     6481 ctagatcata tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt
     6541 ccaacagtcg cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga
     6601 acaattttaa tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat
     6661 ctaatgtgat accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat
     6721 atgcaccatc atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg
     6781 ctagctgtgt atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat
     6841 agccggaaat ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag
     6901 aagaagtagg ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac
     6961 ttaatactgc gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag
     7021 aacgatccac atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct
     7081 gcttgatact ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc
     7141 catctggttc caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt
     7201 cagcaaagaa tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg
     7261 gtttgctttc tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag
     7321 gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct
     7381 gtgataggat aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta
     7441 ttccattata ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga
     7501 actgttcaaa ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga
     7561 catgtatccc ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta
     7621 tcctactgtc atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct
     7681 ttttctttat ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt
     7741 ttacttcata tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg
     7801 ctttcatatc tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc
     7861 tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc
     7921 catcgttccg acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc
     7981 accatttcgt tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc
     8041 gcgtctaatt gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa
     8101 tgtatagcgt actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc
     8161 tcgagaaatc gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc
     8221 ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt
     8281 ctttttcata ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc
     8341 atattttggt attttttact tttatttttt gttttcattc aggttgctaa cctttttcta
     8401 ttttaacacg caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt
     8461 aaatctcttt gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta
     8521 atttctcaaa cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa
     8581 gaccttcttc catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag
     8641 aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca
     8701 attgataaga aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc
     8761 cccattgtcc ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt
     8821 ttttaggcga catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa
     8881 agtcttcctg attcgggtcg ccgatccctc tgatgctaaa gaatgatttt ttaggaacct
     8941 ctagtatttg cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt
     9001 ccatttttct tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt
     9061 gacttggcta atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata
     9121 aatcgtacag ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata
     9181 gaaatcactc tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac
     9241 agctgctttt agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga
     9301 tcgagacggc catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg
     9361 cacaagtcca atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt
     9421 ctacttattt gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt
     9481 tcttggcagg tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc
     9541 gattgctcca aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga
     9601 catttgcccc cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca
     9661 ttgctccagc aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc
     9721 atatcttcct gtttcttgtg cccgttttga ttctttcttt gattatcggc tggtttgcta
     9781 ttcccgagat ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt
     9841 gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga
     9901 ttgctttaat catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg
     9961 agcatccact gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg
    10021 gcttcttagt ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc
    10081 agatcgttca aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg
    10141 ttggtgcttt gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc
    10201 caatttttgc tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag
    10261 gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat
    10321 tatcttacag caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag
    10381 acgggaacgc aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag
    10441 tagcggtcat tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta
    10501 cattaggatc aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc
    10561 gattcgccta ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg
    10621 catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa
    10681 aattgccatt atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc
    10741 aaaattcaat gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg
    10801 gattatctcc ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga
    10861 cggcgctgct tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc
    10921 acaatcaggg acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc
    10981 cgagaaacga aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca
    11041 gacatctcaa gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg
    11101 tcgtctggat ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat
    11161 ggagcagatc caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg
    11221 accgtttgga gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc
    11281 tttattacaa gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc
    11341 atttcagaca tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc
    11401 tgatttcatt cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca
    11461 aggctgggat ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca
    11521 gttcttgact gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg
    11581 tgcttatacg tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact
    11641 ggctgctagc ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt
    11701 agccaacttc ttaaatactg gtcattggcc tacggaagat tatcaaaagg acatctccac
    11761 ctatattacg aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat
    11821 attgcgcatg gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt
    11881 ctccaaactt tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa
    11941 tcgtaaacga atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga
    12001 aacaggaaaa tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa
    12061 ttctgccgtt tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt
    12121 tttatcatcg aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa
    12181 ctctcgaaaa gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg
    12241 ttcaatgact agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt
    12301 tgttgctaat gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg
    12361 cggaagcgaa tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc
    12421 gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc
    12481 tcctgttact aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt
    12541 aaggaaagaa gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga
    12601 aggttccaag gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa
    12661 gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc
    12721 ggacaaccca aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc
    12781 accgtggcag gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc
    12841 tttttttaat tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc
    12901 aaacaaaatg agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata
    12961 aagatctccc tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc
    13021 tggctgaaaa ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa
    13081 atgactttgc acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg
    13141 gcgcttgcga ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt
    13201 gtctaagtcc tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt
    13261 gaatactagt gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa
    13321 atctgtgatg atcgttcctg gactcccttt tgctagttga taagctgtct tcctatcaaa
    13381 aacttcatga acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa
    13441 cagtggattt ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc
    13501 tcctcattcc ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg
    13561 tactgctgcg ctgtgggaag caaacgagtt at
//
LOCUS       DACDVT010000064        13381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDVT010000064 DACDVT010000000
VERSION     DACDVT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.065"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..765
                     /locus_tag="IWL52_001993"
     CDS             64..765
                     /locus_tag="IWL52_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9778530.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="IWL52_001994"
     CDS             762..1883
                     /locus_tag="IWL52_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9778531.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="IWL52_001995"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="IWL52_001995"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9778532.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="IWL52_001996"
     CDS             3262..4170
                     /locus_tag="IWL52_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9778533.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="IWL52_001997"
     CDS             4205..4477
                     /locus_tag="IWL52_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9778534.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="IWL52_001998"
     CDS             4488..5534
                     /locus_tag="IWL52_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9778535.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5785..8394
                     /gene="clpB"
                     /locus_tag="IWL52_001999"
     CDS             5785..8394
                     /gene="clpB"
                     /locus_tag="IWL52_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9778536.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8545..9216)
                     /locus_tag="IWL52_002000"
     CDS             complement(8545..9216)
                     /locus_tag="IWL52_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778537.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9209..9703)
                     /locus_tag="IWL52_002001"
     CDS             complement(9209..9703)
                     /locus_tag="IWL52_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9778538.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9723..10943)
                     /locus_tag="IWL52_002002"
     CDS             complement(9723..10943)
                     /locus_tag="IWL52_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9778539.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11090..12925
                     /gene="lepA"
                     /locus_tag="IWL52_002003"
     CDS             11090..12925
                     /gene="lepA"
                     /locus_tag="IWL52_002003"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9778540.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13121..>13381
                     /locus_tag="IWL52_002004"
     CDS             13121..>13381
                     /locus_tag="IWL52_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778541.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLL"
BASE COUNT         4416 a         2418 c         2787 g         3760 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa gaaaaacgtt
     5581 atggataaga taattttacg tttgtttggc tcttttttta tatacataca atcaattttg
     5641 tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac
     5701 caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac
     5761 ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa
     5821 gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc
     5881 catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc
     5941 ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt
     6001 gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg
     6061 aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt
     6121 attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga
     6181 ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc
     6241 tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag
     6301 caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc
     6361 atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta
     6421 gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa
     6481 aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa
     6541 ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat
     6601 ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa
     6661 ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg
     6721 atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa
     6781 cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg
     6841 cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta
     6901 gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca
     6961 atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg
     7021 gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa
     7081 aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa
     7141 cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat
     7201 tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa
     7261 aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa
     7321 aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag
     7381 tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg
     7441 aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga
     7501 gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct
     7561 ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc
     7621 ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg
     7681 gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct
     7741 cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat
     7801 ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc
     7861 ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc
     7921 aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg
     7981 actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga
     8041 aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc
     8101 ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa
     8161 catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct
     8221 tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca
     8281 ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact
     8341 ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc
     8401 attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca
     8461 aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc
     8521 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
     8581 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
     8641 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
     8701 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
     8761 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
     8821 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
     8881 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
     8941 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
     9001 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
     9061 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
     9121 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
     9181 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
     9241 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
     9301 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
     9361 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
     9421 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
     9481 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     9541 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     9601 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     9661 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     9721 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     9781 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     9841 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     9901 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     9961 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
    10021 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
    10081 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
    10141 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
    10201 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga
    10261 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
    10321 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
    10381 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
    10441 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
    10501 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
    10561 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
    10621 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa
    10681 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
    10741 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
    10801 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
    10861 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
    10921 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
    10981 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
    11041 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
    11101 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
    11161 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
    11221 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
    11281 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
    11341 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
    11401 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
    11461 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
    11521 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
    11581 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
    11641 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
    11701 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
    11761 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
    11821 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
    11881 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
    11941 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
    12001 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
    12061 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
    12121 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
    12181 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
    12241 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
    12301 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
    12361 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
    12421 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
    12481 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
    12541 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
    12601 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
    12661 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
    12721 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
    12781 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
    12841 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
    12901 ggatgagcaa gatagtgtga aataacttat aatcaatcga aaaacctatt aaataaacct
    12961 ttttgagtag atgcaacact gaggattatc tgtataaaag attttaaact gtcatttggt
    13021 tatcacggcg atactaaaat taacaaaatt ggaatatttt tcttgaagat ttttagctgt
    13081 cagtatatga tgaaagcatc tcataggagg tgcttttttc atggttgata attcaagtgg
    13141 aagggttctt aaatctctaa gaaaagagaa aaaactatct caaaaaaagt tagctgattt
    13201 agcaggtatt tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa aaatccctaa
    13261 agacgtagat aatactctaa gcaagattct gaatatagaa actctaataa aagatgagga
    13321 agataagatt gagattctca tcggtaaact aattgcatac agagatatga ataagttact
    13381 t
//
LOCUS       DACDVT010000065        13353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDVT010000065 DACDVT010000000
VERSION     DACDVT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.066"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            620..1483
                     /locus_tag="IWL52_002005"
     CDS             620..1483
                     /locus_tag="IWL52_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9778542.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1612..3234)
                     /locus_tag="IWL52_002006"
     CDS             complement(1612..3234)
                     /locus_tag="IWL52_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9778543.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3483..4004
                     /locus_tag="IWL52_002007"
     CDS             3483..4004
                     /locus_tag="IWL52_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778544.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IWL52_002008"
     CDS             complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IWL52_002008"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9778545.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6085..6903
                     /locus_tag="IWL52_002009"
     CDS             6085..6903
                     /locus_tag="IWL52_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9778546.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7006..7203)
                     /locus_tag="IWL52_002010"
     CDS             complement(7006..7203)
                     /locus_tag="IWL52_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778547.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7372..7950
                     /locus_tag="IWL52_002011"
     CDS             7372..7950
                     /locus_tag="IWL52_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9778548.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8995..9498)
                     /locus_tag="IWL52_002012"
     CDS             complement(8995..9498)
                     /locus_tag="IWL52_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9778549.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9857..10882
                     /locus_tag="IWL52_002013"
     CDS             9857..10882
                     /locus_tag="IWL52_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9778550.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11205..12083
                     /locus_tag="IWL52_002014"
     CDS             11205..12083
                     /locus_tag="IWL52_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778551.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12559..12675
                     /locus_tag="IWL52_002015"
     CDS             12559..12675
                     /locus_tag="IWL52_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9778552.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         4181 a         2298 c         2545 g         4329 t
ORIGIN      
        1 cgtgtcacag tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa
       61 gcccaatcag atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg
      121 cagagcagct ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca
      181 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
      241 gagctaaacg gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt
      301 attagctcaa aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg
      421 gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
      481 cttgaagctg accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac
      541 cggttaaagt gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa
      601 cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata
      661 ccatgtatta gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca
      721 agagttagta gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa
      781 aaatggtgcg atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg
      841 ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg
      901 ctcttacagt agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga
      961 gatctatgtt tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc
     1021 tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa
     1081 ttatttgaaa gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg
     1141 taggatttcc aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac
     1201 ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat
     1261 ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta
     1321 tgtggcttac gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca
     1381 gataaagatg aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg
     1441 ctatggctgt atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct
     1501 gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca
     1561 tcctatctct tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca
     1621 taactcgttc aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc
     1681 agctcgatcg atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat
     1741 aaattgatgg tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt
     1801 caatgctgtg attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt
     1861 agctttcgaa agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac
     1921 attcactggt ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa
     1981 gaaggtgtta tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag
     2041 atcagtatcg aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc
     2101 ccaacgtact gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt
     2161 tgttgttata tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga
     2221 tatatcatct aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa
     2281 gtcgtttcca atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg
     2341 ttggatatcg tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga
     2401 ttttgatgca ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc
     2461 ttttttggca tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa
     2521 atcataattt cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca
     2581 tactttgttc aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat
     2641 caaaaattct tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag
     2701 caaaagaaca tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc
     2761 accagttaat tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag
     2821 taaaacagct gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa
     2881 ttcagctgct cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt
     2941 ttctttcagc acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata
     3001 gtcttcatag tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt
     3061 aacggaacct gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc
     3121 tgcaccattt gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc
     3181 atcaaatagt ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt
     3241 tcctcacttt tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta
     3301 aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa
     3361 aaataaaagc ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt
     3421 ttgacgttat ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga
     3481 aaatggatga aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac
     3541 ttgtctcatt gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct
     3601 ttgcgatttt tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag
     3661 agctaacagg atataaagga aagatgccct taataatcgg gatcatcgat attctagttg
     3721 gcgtcttttt cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag
     3781 tatggtttat tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa
     3841 gtgaaggata ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt
     3901 tcttattgtt taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt
     3961 ttatgttatt tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat
     4021 aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca
     4081 tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag
     4141 aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct
     4201 tttcatataa ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt
     4261 ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc
     4321 gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa
     4381 aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc
     4441 caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt
     4501 cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa
     4561 gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac
     4621 ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac
     4681 cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca
     4741 ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt
     4801 cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat
     4861 cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc
     4921 agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat
     4981 caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga
     5041 tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga
     5101 caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc
     5161 cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag
     5221 cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg
     5281 ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta
     5341 gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag
     5401 gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt
     5461 tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca
     5521 tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga
     5581 aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat
     5641 ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc
     5701 cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga
     5761 cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca
     5821 taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa
     5881 gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca
     5941 atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact
     6001 aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag
     6061 atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag
     6121 caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt
     6181 tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt
     6241 gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa
     6301 aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta
     6361 tatatggcag ataaaacact gccccatatg cattttggac cacaacatga agcagaagga
     6421 ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt
     6481 cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca
     6541 gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa
     6601 ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt
     6661 tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc gctactagta
     6721 acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat
     6781 gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt
     6841 gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga
     6901 taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta
     6961 tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt
     7021 tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag
     7081 aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa
     7141 gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg
     7201 catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt
     7261 aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg
     7321 ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa
     7381 aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta
     7441 gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt
     7501 tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta
     7561 tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt
     7621 gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct
     7681 ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca
     7741 aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat
     7801 tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta
     7861 gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca
     7921 agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa
     7981 atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat
     8041 gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat
     8101 ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt
     8161 attcacagca ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa
     8221 aagcatgttt acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa
     8281 tttttagaaa agtgtatcga aaaatagata gttagttttt ccacagtgga taacttcgta
     8341 agagataaaa ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata
     8401 tacaacagaa atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt
     8461 ggaataaaaa aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac
     8521 gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc
     8581 ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag
     8641 ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct
     8701 ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga
     8761 ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct
     8821 ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa
     8881 taatatagta gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac
     8941 cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg
     9001 aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta
     9061 tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg
     9121 ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc
     9181 tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat
     9241 ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg
     9301 aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa
     9361 agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt
     9421 aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa
     9481 ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct
     9541 gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga
     9601 agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga
     9661 aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc
     9721 tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact
     9781 aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt tccaattact
     9841 aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc
     9901 ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa
     9961 tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca
    10021 aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt
    10081 tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc
    10141 tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg
    10201 cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt
    10261 accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat
    10321 tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag
    10381 atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct
    10441 ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg
    10501 tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct
    10561 gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca
    10621 gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag
    10681 aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat
    10741 tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag
    10801 tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt
    10861 tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga
    10921 tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa
    10981 ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa
    11041 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    11101 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11161 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11221 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11281 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11341 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11401 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11461 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11521 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11581 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11641 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11701 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11761 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11821 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11881 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11941 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    12001 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    12061 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12121 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12181 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12241 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12301 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12361 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12421 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12481 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12541 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12601 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12661 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12721 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12781 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12841 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12901 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12961 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    13021 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    13081 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13141 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13201 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13261 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13321 tgtgtattgg aattttagat acagtaataa agt
//
LOCUS       DACDVT010000066        13241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDVT010000066 DACDVT010000000
VERSION     DACDVT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.067"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(439..1053)
                     /locus_tag="IWL52_002016"
     CDS             complement(439..1053)
                     /locus_tag="IWL52_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9778553.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1659..2036)
                     /locus_tag="IWL52_002017"
     CDS             complement(1659..2036)
                     /locus_tag="IWL52_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778554.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2336..3217)
                     /gene="galU"
                     /locus_tag="IWL52_002018"
     CDS             complement(2336..3217)
                     /gene="galU"
                     /locus_tag="IWL52_002018"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9778555.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3260..4279)
                     /locus_tag="IWL52_002019"
     CDS             complement(3260..4279)
                     /locus_tag="IWL52_002019"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9778556.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4912..5916)
                     /locus_tag="IWL52_002020"
     CDS             complement(4912..5916)
                     /locus_tag="IWL52_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9778557.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5929..6978)
                     /locus_tag="IWL52_002021"
     CDS             complement(5929..6978)
                     /locus_tag="IWL52_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9778558.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6995..7816)
                     /locus_tag="IWL52_002022"
     CDS             complement(6995..7816)
                     /locus_tag="IWL52_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9778559.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7803..8582)
                     /locus_tag="IWL52_002023"
     CDS             complement(7803..8582)
                     /locus_tag="IWL52_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9778560.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8599..9069)
                     /locus_tag="IWL52_002024"
     CDS             complement(8599..9069)
                     /locus_tag="IWL52_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9778561.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9081..9500)
                     /locus_tag="IWL52_002025"
     CDS             complement(9081..9500)
                     /locus_tag="IWL52_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9778562.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9581..12349)
                     /locus_tag="IWL52_002026"
     CDS             complement(9581..12349)
                     /locus_tag="IWL52_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9778563.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            12549..12773
                     /locus_tag="IWL52_002027"
     CDS             12549..12773
                     /locus_tag="IWL52_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778564.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3887 a         2757 c         2150 g         4447 t
ORIGIN      
        1 tttgtgtatt ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg
       61 taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg
      121 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
      181 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      241 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      301 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      361 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      421 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      481 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      541 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      601 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      661 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      721 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      781 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      841 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      901 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      961 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
     1021 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
     1081 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1141 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1201 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1261 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1321 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1381 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1441 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1501 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1561 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1621 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1681 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1741 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1801 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1861 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1921 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     1981 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     2041 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     2101 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     2161 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     2221 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2281 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2341 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2401 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2461 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2521 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2581 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2641 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2701 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2761 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2821 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2881 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     2941 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     3001 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     3061 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     3121 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     3181 gcccagcggc aggaatgact gcttttttga ccttcacttg ctgttcctcc taaacgacga
     3241 ttaactattg atcatctatt taacgaagag aaaattcatt ctccattttt ccgtcacgta
     3301 acatgatttc ttttgctgct tttttgatat cttctccatt atacaaaaca ttatagattg
     3361 tttgagtaat tggcatctca acacctagct gttgagctag ctctactgct gcttttgtcg
     3421 ttgccacacc ctcaacgacc attcccatgt tttctagaac ttcttccagc ttatgtccct
     3481 gtcctaacaa gttaccggca cgccaattgc gagaatgaac acttgtacaa gtaacgatca
     3541 agtcccccac accactcaaa ccgataaatg tcagaggatt ggcacccatc gcgacaccaa
     3601 gacggctgat ttctgccaat cctcgagtca tgattgctgc ttttgcatca tctccgaatc
     3661 ctaatccatg gatagcgccg gcaccaattg caatgatatt tttcagtgct gcaccagttt
     3721 ctacgccaat cacatcatta tttgtataaa tcctgaaata atcattcatg aacagttttt
     3781 gtacataggc tgctgctttt tgatcaatac ttgcagctgt aattgttgtg atatcatgta
     3841 ctgccacttc ctcagcatgg ctaggaccag ataatacaac aattgcctgt cttttttctt
     3901 ctggaatttc ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt tctagtcctt
     3961 tacttgcatg tataatgatt ggcttagtat tcattttacc aataagttcc tgtgcgactg
     4021 aacgaatcgc ttttgttgga acaacaaata aaatagcatc tgcgttatcc actgcttctg
     4081 ccaattcttt atatccgata atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat
     4141 ttgtatgata cttattgatt tcatcaatct gttcaggtac atttccccaa atacggacat
     4201 catgcccgtt ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa
     4261 ctgcgatttt ttgtttcatg gatattcctc caaataaata attgataatt tccattatag
     4321 tatacatttc ttaaagttgg gactaatttc cattgttttt aagtgtttgt ttacttcttt
     4381 tatcgttttt acttgaaata atcaatgata tgttacaata gtctacattt tatttttaca
     4441 ctcgttatgc tttgcttgtt cctaagagga agcagttttg acaatgttac taccagcatt
     4501 actattagga ggttaggaaa cttctcctag tgagtattga tttaaaatac ataagagaca
     4561 gattgaacat ttctaaatct gtcaggtcct gcaacggaca gattgtctct gttgacggtt
     4621 gacataggaa cttttgtttt agtcaacatt ctggatacaa aacgctatgt aaactggcgc
     4681 aactcgttgt acttggactt ttactacgga tccatgcaac aactaaatag tactattgca
     4741 taacaaaaaa tggttggcac ccacatgtcc cattaaacag attataagtt gaactgtaga
     4801 taatgcaaaa aatgtgtttt ataacgtaat tgatgccaat attttaaaaa aataaaggga
     4861 gcagttatcg ctgctctctt tattttttat attcttttta tccagtctat cctaataatc
     4921 aaactgacga tagtagcggc gtatctctaa tggatggcag ttcaactgct ccacatgagc
     4981 atcgatgcgt tgtgtcacta catgcatcaa taagaaagac aaaacacgtc cgcgatattt
     5041 ttcactgata ccttctactg gatagtcttt tgtgtctatc aatgtataat tgctgcaaat
     5101 ttttggcaaa agtttagcca cacgttcaga taacactctc tgttcatctt cgcctaaaaa
     5161 taatgtgact ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa actcggcagc
     5221 atggatagat ttcgatggaa gccagctttg ttcttcccag taacacatgg catatgaata
     5281 aacagctccc cattgattcc ctgctccaat aaaataatgc atactatcac gtcgatgttt
     5341 ttctgcaaat gataagccaa atgcatctgc ttttttctct gcttccacta accctgttgg
     5401 taaataacgt tcgagctgtt catagtattc tgaataagct tcgaattctc cattcaaata
     5461 catcaatcga tctgctgcca taaaaaattt gatttgttct gttccaggag ctggataagt
     5521 gaccacaaaa tcgcatagct gagacaactt actgttcgct ttatcgataa atccaatcag
     5581 tgtcgctcct actttcttga tttgttctac tgctttcact acttcttgag ttgtaccggt
     5641 aacagaggac aaaataacga tagaatcttt tgttaatctt ttgtttccag tagtgtaata
     5701 ttctgcagca tgctgtacaa agacaggcaa gttgcttttc ccattcatat aagtgacagc
     5761 ttgcatcgag gaagcatacg ttccaccgat tcctagataa tagacagcgt caaatttttc
     5821 cgcataaagt ttatcgatga tttcttctac ttgcggacgt aaagctagtg cgccttttat
     5881 tgctttgatt tgttcttctt cattgaattt cagcatgatt tccacctctt ataatttact
     5941 tttcaagact gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac ctttagctac
     6001 tgcggtttta taagcgaata gttgaaacgg aacgacaagc gctaatacag acagatattc
     6061 atcacattct attgctaatc caagtgtttt ctcgtttctt gctttttttg tggtaatggt
     6121 gagagtctct ccaacgtacc gactcatata tctttgcaac ttttgcgaac gttccttatt
     6181 tacactttcc ccttcaataa agaatagcaa atgcgaagca tctgcctcca ggtatgggcc
     6241 gtgcatataa gcttctaact caaatccttg tgacggaacg cggattgttt cagtcaattt
     6301 cgtctctgct tctttagctg ttccccagtt tggaccatat ccaatggtga cgaaacgctg
     6361 tgctaaacga aataaacttt gatatccatc aaaaaattct tccgtttttt gaataatcgc
     6421 tggtaaatgg ttgattattt tgcgcaactg ttgcatgtag tcttgttcta tttttttgct
     6481 gattttattt ttacttattc caattcctat ccctaagagt agtaactgta aaacagtcgc
     6541 agaatagcct tttgtaacga agcctactga ttctaccccg gcacctaaat caataatttg
     6601 tgcagcttct aaagcaagtg gactttgcac gtcatttgtg atagcaacaa caggtaaccc
     6661 ttgtttttgt atcattttta ctgcttcaac agtagaagca cttttcccgc tttgtgacac
     6721 agcaatcaca gtatcgattg aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc
     6781 ttgaatatcg acagtaagat caccataaga ttccaatgct ggcttcgctg ctaaacaagc
     6841 gttataagat gaacccgttg ctaagatcag taaattcact gtttttttca tatgttctct
     6901 tgtcgaaaaa tcattctttt tcaagatcgt acttagtact tccttttctt catagatata
     6961 atcttgcatc gtaaacattt tctctgctcc ttttttaaaa aatccctatc cacgaaccaa
     7021 aaattccaaa gaaagctagt ccgatcaaga tcgttaatgg tttcacttct ttcttcaata
     7081 gataaaacac tatgccaaat gctccaagac ataagatatt tggaacgata tcatcaaaaa
     7141 tactttgaac agtcacagca ttttctcctg aaccgatttt taaaggaata ttgatggtga
     7201 tcatcgtggc agtcattgct ccaacgacca tcagtccaat aatagacgcg cctaatgtca
     7261 aactttccat cataccgttt gcctggatct ttttcaaaaa acccgttcct aagcgatatc
     7321 cccaacctgt tgctagatag cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca
     7381 agatagggcc taaaatattt ccttgcatgg ctaaagaggt acccacacct gtggcaatca
     7441 atcgcaaagt tccccaaaag aaagaatctc ctaaccctgc aagaggtccc atcaaagatg
     7501 ttttgatact atcgatcgca gtcacatcaa attcaggatc atccttattt tcttcctcca
     7561 ttgcagccgt gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta
     7621 gatgccgttt caaagctgct gccatttctt ctttcgactg atataacttt ttcaaaatcg
     7681 gaatcattgc ataaacataa gccatattca tctgacgttc ataattccaa gaaaactcca
     7741 tttgaaaaga acgccaaaat acgtgattta gctctttttt cgttattttg ctgtttttga
     7801 tctcagaatt cttcatcttc ttctacctcc cctgcagttg tttgaagtgg tagttgacgg
     7861 acattcataa tattgactac cacaactgct aaaatcgcac caaaaatagc tactccggtc
     7921 acaggaatct ctaagtaaga agctatagca aaacctaaga aaaaatatgg tgcaacttgt
     7981 ttgttgatca gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa ccctgtagct
     8041 actgacaacc ccttttgaac gaagtcagga atcatattta atagatttcc gatcgtattg
     8101 cttcccacca tataagaaac cattaccaca acaccaagca tcaaggaaag accgataccg
     8161 gcaagcaagt gcatatgctc gactccttta taatttcctt cttctgcata attgtctgct
     8221 ttatggttca taattggaat caaaagccct aagtaaatat ttttcaatat caatgtcaac
     8281 gtagcaatcg gcaaacctag taaaagcgcg gtttctgtac cggctccagc tgtaatagca
     8341 aaagccgtcc ctaaaatccc accggtcaca acatcaggtg gaatcgaagc acctactgag
     8401 aacgatccga taaatgctaa ctctaaagtt gcgcccataa tgatcccagt ttttacatca
     8461 cctaacacga ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa cgatgtccct
     8521 agtgcatatt ctgattgtgc gacaaatgca acaattccta ataagactgc ttgtacaatc
     8581 attgaggttc ttcctttctt acaaaacttc tcttacatcc acttttttat ctgctggtac
     8641 ttgacggatt tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc
     8701 tggtaaaata tttaccgctt ttccgatgct gcgagtacca ggttttgcct tgatccctcc
     8761 taagttgatg gttttgattt ctggatacgc ttctgccagc ttatatgcat cctcgatcga
     8821 ttccactacg atgaataact tatatttatc tgtcaccccg cttttcaacg cggcaatcga
     8881 gtcatcaatt gatttgatca ctagtttgac tccttgtggc ttggctaatt tgatcgttgt
     8941 ctttcgtatc tcattcgtag gtacatcatc attagcaatc aaaatacagt ctgcgcctat
     9001 tgtttgtgtc catgaaaaag ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa
     9061 tacaatcatt tttcttcctc ctaaaattcg tcttcttcta ttttctgatt gagccgctcg
     9121 ttacaaaact ggatcatttt ttttgaatca gttagcacct gctggatcaa aacagttgtc
     9181 tctacttcag cgtccaaccg agatactagc tcaatcaata gtggcaaatt caatccggca
     9241 acgagataga catgatcccg attgatatat tgtaagaatt cgttgttgac acttccgcca
     9301 aaaagatccg tcagaacaat gatttcttta tctttgtacc tcataaaaag ctgccttact
     9361 gtttccgcta aatcaaaatt ctcatcagta tagcaatcga atacagtaat agaatgattt
     9421 ttcccataga tcagctctaa tgaatctaat atccctgaag caaattttcc atgggaagct
     9481 aagataattt ctctatccac tttttcatct cctcttttca cttacacata ctaaaaaagc
     9541 aagttgcatg ccaaaattag tggcaacaac ttgcttatgt tcaaaattct tcttctatcg
     9601 agagaaactc tgtatcttca ctcaaaatat catagatata ggcaatctca gaattgggga
     9661 tcatgacact ataatgctct tcaatgacac taaacgcaga tttaatagca gataaccctt
     9721 ctttttgaca ctgcttccat ttctctaatc cttgataggt ttcgattggc atttgtcgga
     9781 tcaatcgttc aatgagacag ctcacatgga catacaacgc taattttttg ggattgctga
     9841 tgcttcgatt caactgcacc tctaattctc tcataaaatg atcgatttct cccatcacct
     9901 tctctgtatc tagaatagtc acggaatcca gtactttttc caatgaaaaa ttacgaatga
     9961 tgcgctgatt gaatatttcc ttctctgaag cattcatagt agtgtccaac cattcagcta
    10021 gtacagacat cttttccccc gagatgaggt cttctaatga aagatagtcg atattggtga
    10081 tatcaggatc tgtggtgcca atcattccta cgacttcgta tatggaaaat aatgggtcac
    10141 tgtttttctt atcttttaat tgctgatatt catatgggat gattttcaac tgacaagccg
    10201 taggtaagct tttttctagc agttcactta agtggaccgc cgtaccgatt ccagtgaagc
    10261 aagtagtaat gagtgctttg ggtttgtttt cctctggata gagcagtcgc cattctggct
    10321 ggatctgtcg aacagtctct tctgctatct cttctaatga tacatttttt tgcaggcatt
    10381 cccctactgt gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc ggtacggata
    10441 actgtttttt aaaaaactga tggatttctt tcagagatcc catgtctact aatatgatca
    10501 atccatttga aacatcgctg ttttcgcaat aatcaaggat ttctgaagca atctgctgtg
    10561 gtgtgacatc tagtggcata tcaaatgatt caaatagatc ctcttgtaat aaacgattta
    10621 ctacatttgc catactgcta gcagttgcat aaccgtgagc agcaatcatc gcttttggca
    10681 gtcgtttttc ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa atccgatcca
    10741 tacttgtgtc ttcaatatct aatttgatct tacttaattc cagaattcgt tggacatagt
    10801 gataactaga tggataagac tgagaaactt gttgttctaa ttctttgatt agttggataa
    10861 tatcatgttc ttccggtaac catttgatag tgcttcgctg atagagatag taactcaagg
    10921 catagacact gtttccatcg aatttgattt gataggcagt ttccatttgt cgaaaggtct
    10981 ctcggataga ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa
    11041 acagcaaaaa atcaaataat tgaatcactt cttgctttaa ttttccttcg caagaatata
    11101 gcagacagtt atttttcttg aaatcaatta ggatttgctc aaaactttta acgattcgct
    11161 gctgttcggg ctgttgcttc tccattaatt gctctaaggt ctggtcttgc gtaatataga
    11221 ttccttgctg tgctgtattt ttagtaatag gctgcgcaag tagttctttt tgcaaatgat
    11281 ggatcgtgac atggatttca ggctgttcac gctgttcagc atatgctttt gctgctgaaa
    11341 ctttgacgat atttttcagc tctcctatgt ttccggataa tttctgactt gttaaaagtg
    11401 acaaagctgg tccactgact gacaaagggc tccccatttt tctttgttct tcgagaaaaa
    11461 aagagtaaat cagttctagc cgttcattac gactacgctg attaagtgca ggtagttcga
    11521 ttcggatagg tattcttcga atgaaagttg ttaagaaatg actagtaatc tcttcagtcg
    11581 tagcaaaaaa gagacgtgtt ttgatccggc gtgacaaatt aggttctccc atacgataaa
    11641 tcacaccttg atcaagatag gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta
    11701 cctcgtctaa aaacaagatc ccaccatctg ctgcttcaaa agcccctttt ttattttctt
    11761 ctgcaccagt gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa agttcaggat
    11821 tatctgcata ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct agcagatcat
    11881 gaagcagaca atactgatag accaaattaa ctaaataact tttacctgtg ccactttcgc
    11941 ctgtaatcaa aagtggtaat ccgccatctg gatagtaaag agctgttttg atttgttcga
    12001 tactttcttt caaactggcg tcgtatccga tcataaaaga gaataagtct tttggttgcg
    12061 cgaaaaaagg ctgttcctct ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact
    12121 taaaaaattg tttttcaaaa gcttctttgt ggaaataaat cactggtcga gaattgattt
    12181 tgactagtag ttgctcatca tttaactggt tcaaataatg actgactgtg tttcttttta
    12241 ccccaaaatg atcagctaac ctttgtgctg taaagacctc gttcatatgt tctaaatcga
    12301 aaaaggctgt ctggttctct aagtaacata aaatttcctt cttcaacaac ttattcaccc
    12361 ctcctccatc atagaaaacg gattcatttt gtcaataaaa tctttaataa agaattgaaa
    12421 aatatatctt attatagaca gcagcctact tatcccttat aatttttatt ctaaaaaacg
    12481 tacagttcct tccagttttt atactggaat ttcaacatat gaaagaataa ggaggctatg
    12541 actattcaat gaaatcggat aaatataaaa acaggaaaca tacttcctgg aaaacttttc
    12601 gagaaatcat cggatcttat ctcgctgtag ttgtattttt gcctttgctt tatatggcta
    12661 tgacagaaca aactcgtttt attggcgtag atacgcaata tatagccgaa acaggatttt
    12721 tattcatctt tatcttgatt tttgcgttct ataaatcctt ccatacaaaa taagattcac
    12781 agatttcaac tttcctgtct gtttttagct attacaaatc cttatatgtg gtataacaac
    12841 aatttattgg cataatgagg aaaaacgagt tatgatgctt tcagagagta aagaggtact
    12901 gttgtcacca tcctttttat tgattacgta caccactctt agttttttcc cattgattac
    12961 gaaaacaagg tagaaacatt gatgttccta ccttgttttc tttttttatt ttcagatcaa
    13021 aatctcaagt agcttttgac ttgatgtcgg gaggttgtct tcgttccatc aaacctgata
    13081 gaatctgcgc caaaccatag ttttctccac tattatttat tcctctaaga tagtacccac
    13141 aataaacgaa taaatatttt tctcattttc atactccttc tttgaaaatc aattagattt
    13201 ctctcaaccc acttttttac aaaaattacg tagcaaaaaa g
//
LOCUS       DACDVT010000067        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDVT010000067 DACDVT010000000
VERSION     DACDVT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.068"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(78..150)
                     /locus_tag="IWL52_002028"
     tRNA            complement(78..150)
                     /locus_tag="IWL52_002028"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IWL52_002029"
     CDS             531..1100
                     /locus_tag="IWL52_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9778565.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IWL52_002030"
     CDS             1087..1947
                     /locus_tag="IWL52_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9778566.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IWL52_002031"
     CDS             2137..2841
                     /locus_tag="IWL52_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9778567.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IWL52_002032"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IWL52_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9778568.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IWL52_002033"
     CDS             4678..5994
                     /locus_tag="IWL52_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778569.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IWL52_002034"
     CDS             5995..6864
                     /locus_tag="IWL52_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778570.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IWL52_002035"
     CDS             6919..7728
                     /locus_tag="IWL52_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9778571.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IWL52_002036"
     CDS             complement(7831..8460)
                     /locus_tag="IWL52_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9778572.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IWL52_002037"
     CDS             8630..9922
                     /locus_tag="IWL52_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9778573.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IWL52_002038"
     CDS             complement(9998..10660)
                     /locus_tag="IWL52_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9778574.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IWL52_002039"
     CDS             10885..11169
                     /locus_tag="IWL52_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9778575.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IWL52_002040"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IWL52_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9778576.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2264 c         2833 g         3679 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13141 tccatt
//
LOCUS       DACDVT010000068        12694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDVT010000068 DACDVT010000000
VERSION     DACDVT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.069"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            70..1521
                     /locus_tag="IWL52_002041"
     CDS             70..1521
                     /locus_tag="IWL52_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9778577.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1870..2541
                     /locus_tag="IWL52_002042"
     CDS             1870..2541
                     /locus_tag="IWL52_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9778578.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            3089..4528
                     /locus_tag="IWL52_002043"
                     /pseudo
     CDS             3089..4528
                     /locus_tag="IWL52_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4873..5040
                     /locus_tag="IWL52_002044"
     CDS             4873..5040
                     /locus_tag="IWL52_002044"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9778579.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            5299..5514
                     /locus_tag="IWL52_002045"
     CDS             5299..5514
                     /locus_tag="IWL52_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778580.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5702..5989)
                     /locus_tag="IWL52_002046"
                     /pseudo
     CDS             complement(5702..5989)
                     /locus_tag="IWL52_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6176..7087)
                     /locus_tag="IWL52_002047"
     CDS             complement(6176..7087)
                     /locus_tag="IWL52_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9778581.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            complement(7106..8110)
                     /locus_tag="IWL52_002048"
     CDS             complement(7106..8110)
                     /locus_tag="IWL52_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9778582.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(8107..10215)
                     /locus_tag="IWL52_002049"
     CDS             complement(8107..10215)
                     /locus_tag="IWL52_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9778583.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            complement(10220..12667)
                     /locus_tag="IWL52_002050"
     CDS             complement(10220..12667)
                     /locus_tag="IWL52_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9778584.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
BASE COUNT         3910 a         2412 c         2348 g         4024 t
ORIGIN      
        1 tcattgaaaa tatttatcat aatttttaat agaaaacact attaaagaga aaaaagagag
       61 gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc
      121 tttatagcaa cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa
      181 gaaagaaccg agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt
      241 ttgacaactt tatcaagtga aagtagtacg acttcaaatg ctgagtcttc aacaacttca
      301 tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt
      361 agtacgactt caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca
      421 agtgccgaat cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca
      481 agaccaaacc atatagagtc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa
      541 aatttttcga caccacagca aatagaatca acagaatctt tccaatcaca accaatacta
      601 tctggtaatg aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa
      661 attggtgagt ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt
      721 gcacaagcga tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac
      781 aatttatttg ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa
      841 gatttgggta atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat
      901 gaggaaagtt ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat
      961 ttttatgcag gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt
     1021 acaggacggt atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc
     1081 tatgatttaa ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac
     1141 caagtgccta ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt
     1201 caccgaggac ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt
     1261 aaggttgtta aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa
     1321 gatggatcaa ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat
     1381 gttttgcaag gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac
     1441 cttcatttgg aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa
     1501 accttattat tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt
     1561 ggattcttag acctttgatc aggatattga gactggaatt gatatctcga ttacgatgag
     1621 tttgacaaat aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct
     1681 catattttga gcaggtttta ctagatgaaa acaatttatc tatcttgagg atttcgtgta
     1741 cgtatcgttc agtcttgtat tataaattag tcataaaatc agactaagaa aaattgaaaa
     1801 tatttttttc tgaattatta aaagttaatt gaaattatat ggtacttctt tcttgggagg
     1861 gtaggtgcag tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat
     1921 ggatataaaa aatatcgtta cctatgtgac tgtgggaatg aaacgattgt atatcgatca
     1981 aacttactat caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa
     2041 tataaaaatt tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg
     2101 cgaagtgaag gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta
     2161 tcggcaaagc gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag
     2221 catcacattg aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac
     2281 tgttcatgga aaacaaaatg ggtttgtcaa tgtgagtgtg gcagtatgtg tgagataagt
     2341 tatagtaact tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca
     2401 aggacatgtg ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat
     2461 acaagtggtg ttaagggagt ttatcgttct aaagagaaat ggattgcgta tataacttta
     2521 aaaaaaagag gtactattta ggatcgtatg attctattaa gagtgcagct caagttagaa
     2581 aagatgctga aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat
     2641 tattgtggat aataattaac agtgatatat aaacatctat aatatattat tcatactatt
     2701 gacatttagt ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg
     2761 gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat
     2821 tttcttacgt ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt
     2881 tagaaattgc tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa
     2941 taaagcatct caaacttaca tggttacatg agaataacaa atcgaaatta ctcaagcttc
     3001 ctatggctag agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct
     3061 acacgatcat tagaaaggaa atcctattat gcatagttat caagaagatg caaaagtcca
     3121 aaaaaatcgc tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga
     3181 tggcagtatt gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa
     3241 tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt
     3301 tggtaaaatt ggcgatagct gtgggaaaat caaagtattt cggattggta cgctgatctt
     3361 tgtcttgggc tcgctgttgt gtgggttcaa tcattcgcta ggtatttaat tgttcgcacg
     3421 ggtggtacaa gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga
     3481 agtctttccc atacatgaac gtggtcgggc attgggagca atcggtgcgc ttgtttcatt
     3541 aggctcgatt gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta
     3601 cattttttgg atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt
     3661 accaaaagat atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc
     3721 ggctacgatt atcagccttt ttggaacaat ctttttgggg caagaaagtg gctttttcca
     3781 tccagtcccc cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt
     3841 tgaaaagaaa gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac
     3901 catgagcttg attacagcct tgttgatttt ttcttccaac ttctttgtta atgtagtgat
     3961 tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc tatgccggac tgttgatgat
     4021 ggtctttccc ttgatgatgg tcgtcggggc gcctttaagc ggatacctga ccgaccgggt
     4081 tggcccggag atttttgtat tagtaggctt gggtttattg tcggtaaccc aagtcatgta
     4141 tatgtttttg tcagtggact ctcctttgtg gctgtacatt gtcgctactg ccatcatggg
     4201 tgccggcaat gccttgttcc agtccccaaa taatacgatg gttatgagtt ctgtcactat
     4261 cgaaaatttg ggactggcag gctccatgaa ttcctttgcc cgaaatttgg ggatggtcat
     4321 cgggatttcg ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccaacg
     4381 agtaactaac tatatcgcag atcggccgga tgtcttttta tatggaatga agattacctt
     4441 tttaggcagc tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa
     4501 acaaggaggt attcagaaaa atgtctgaaa aaccattgtt gaagaatttt tggaggcctg
     4561 cgtatgggtg agtgggattt ccatggatga tggaagatga agtgaacttc tgaaaaccga
     4621 aaaactcaag tattatcttc aaaatactag tactcaatta gatgctcgat ttgattacaa
     4681 gaagactgcg aaagccactg gcaaccgcaa gctaggtatc caccgatgga ctacgtttta
     4741 gaggcaactg gttatgaata cggagctaac gctctcatct gcataagaat gcaacaccca
     4801 gattatgtaa agattttcga tacttctgtc aaaaaaatca tcaagataga taaatgattt
     4861 ccgtctagtc aaattggttc agaatgtgga cacacagatg gtaagaagtc attcacaatt
     4921 cgagaatgga cttgtcctat ttgccgtaca catcatgatc gaaacgtcaa cgttagtatc
     4981 aatattttga tcaagggcta tgtatacaag cattgcctta gaaaacatca agaaccttag
     5041 gaacaacggg gatagattgg ttaataagag atatcgctgt tagtaaagac ataggcttgc
     5101 aagtatactc ttttatcaag aattccgtgg ctttagtcat gataggttta atactatgaa
     5161 aattttcttt agcatttttg atttaggttc cttttctaat tataataaaa tgaccactac
     5221 agtaacctac tgaaatatat aaacagccac tgtagtacca ttttcaaagt agtctatgag
     5281 aggagaatct tcattcttat gaacgctgga attgcggata tgaatttaat aaaaaaaacc
     5341 cttaatgatt ttacttccaa ttcaatatca aaaggtactg gtatcaattt aagcacaata
     5401 aaaaagctga aaagtggtga aagatcagtg gaaaagctga atcttttaga tgctataaaa
     5461 ataacggaat ttgctatgaa aaatggaaaa gctgagattg aaatatggag ataattatcg
     5521 tctagtgaac tacttgccac ttagctgaat ttaacgatcc ttagtgcttg aagtgggaga
     5581 ttcttgggaa tagagcatac ttgcaagctt atgtctttat taacagcgat atctcttatt
     5641 aaccaagcta tccccgtagt tctaaggttt ttacttttat ctaagctaat gatcgtattc
     5701 aaaggaaatt tttgaccctt agaaagtctc attttatcag acatgagctt aggcattgct
     5761 gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt agtctttatc
     5821 acgatttcac tcaactaagg taatgtgatg tgattctgat attctttcaa agatagtttt
     5881 ttgcataagc taacattaca tcatcaatga gatgtttaca atgtttcact actaaaataa
     5941 ggtggtaaca aagaagaaat aatgaatgat tatttgtatc taattccatg gtatatcaac
     6001 catttcttat atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt
     6061 gtcggttttc gagttaccga acggcattca tcacccacct atagaggatg ggcgacttct
     6121 gcctgaaata agttaaacta cttataactt ctcataaatt tagattgagt cattattatt
     6181 taacatcaat gattttccag tttccatttt tttgtaatat taatttgtac tgaaagtagc
     6241 tagtggtttt tgatacatta tcaaggtatt ttatctttgt actcactaga attttttttc
     6301 cgttttgtag gtaaactgga tcctctaact tcgaaaactt taagtcaata ttgattggtc
     6361 gcatcgcatc attttcaaca taatactcca attctttttc agaagctgat ggatataatt
     6421 taaagaacgt ttccaaaaac tcagcgattt cttctgtagt tttggcatca attttcgaat
     6481 cattttctac ttgttttgtc ttgtattttg ccctatcagg ttttgcaatc atagttggca
     6541 aactcgttac tatataattc ctgttttcat cttcaaagat cgtcactcgg tatgcagaag
     6601 aaatcgattg tgcctttttt ccctctgtaa tcttttgctc cactaaaaat gaaacgacaa
     6661 attcctccga gctcttttca acatctaaga cttttactga ctgaacttcg gaattagtgg
     6721 gaatatctgc tcgaaccata tcttgactta aagcgaaacc ttcttctgta aggtactgtt
     6781 cgaggttgtt cattctcttt tcaattgctt ctttgttatt tttccacgaa aaatattctt
     6841 tcgaaaaatc cttcgtaaag ttttcaatac cagaagtatt cactactttc tctttaataa
     6901 ccactttttc atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag
     6961 taagactaca agataataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg
     7021 atacgactcg ttcttttttc ttttttaggc tcttttcctt ttgattccgt tcaattttta
     7081 tcttcattct ttttcctctt ttctcttatt tgtaccgtcc agcaccgata agatgctgct
     7141 gccaatagct ttctgttaga ttcgtccagc ctactggatc tcctgcatga aacatgcgat
     7201 tctcccctgc ataaataccg acatgggtca cataagtacc ggcatcataa gtcgaatgaa
     7261 agaaaaccaa atcccctggt ttggcatctt ttaggtcaat atgggtcatg gcatcatact
     7321 gatcttgagc cacccgagga agtttcagac caactttggc ataacaccat tgagtcaagc
     7381 cactacaatc aaatcctgtt tcgggtcttg acccaccaaa tacataagcc gttccttcat
     7441 acttaaacgc ctcgtcaaaa atcccttgga ctgttttatc gtcaaactgt gttgtagtta
     7501 ggtattgctt caccaataag caataaaaca tattgccata gttatagcgc catccaccat
     7561 tttccttatt cgcgatggga ttagggtaat cgattttttg accgcaagat ttttcctttg
     7621 aaaaagattc agctaaagca aaactatgct tcttcccgtt ttttgccaca tagtccaaat
     7681 aaccaccgcc aaagttatag gcctgaatga tactatcttg atcacaacct aattctttgg
     7741 aacgcttaac caaagacgca aaatagagac atccttgttt gatggatgcc tcggtactta
     7801 atgaattcgg tggtaaaccg gctgactcag aactttgcat cacatcttcc attgtgccac
     7861 cagattccac ttgaataatc gctaaaagaa tcggaacata ctctgttata tccgattctt
     7921 tggcatattt ttccaccatt gcacggtgtt tcaaaacctc ttctgagaca ctactccccc
     7981 caggaggagc acctgaacta ctacttcctt cactgtcttc accaaacaat aagcccacac
     8041 acatcaagat acccataaaa gagattgtta ggaaagttaa cgaaatggca cttaatttct
     8101 ttacgttcat tttttctcac ctgaccttgg tttgggatga gggttcaaac ttttttgtac
     8161 ttttcgattg gttcgtttgg acagagtaga ccgccgtttc acttgttgaa aagaattttt
     8221 cttacttgag gattggttta ttccttttga aatgatttct tggctagcga attttcgttt
     8281 gatttcggga ttcgaatttg ctctcacaat ccgctttggt gctctatcct tttgagattg
     8341 gacctttgct tttatcggaa cattttccct tcttttccca tgaggattcc ctatttcgtt
     8401 caatactttg cgcttttcag ctattttttc gtctctacgt tgttgttgtt tttctcgcaa
     8461 ctctcctcga tttgcttttt cttggaccag tcctcgctta aactcttccg gtgctttttt
     8521 cgttttttca attcctttat ggaagttgta tttcaagttg gttggcgtat tccgtactcc
     8581 atcctttact tgtttggtct tatctttcac caaagcttgg gtatccagaa ccttgccgac
     8641 ttttctcccg gccaaattca ttcgggattg tttcttatta tttggaattg gcttttcctt
     8701 attcaagtca gatggtatct cgtgatcgtt cggtaatcgt gaattctttc gtagagaaga
     8761 accggatggc ttaaatttgg attttgattg ttcctttgcc aacttatttc caactgctgc
     8821 accaccacca aaagccaaag tccgtcccac atttcgattg agttgccgca atgttcgatt
     8881 cattaacatt tgcggttttc gcatgacccg tcttcccatt gattggctat cattactctg
     8941 gagattgaac atattcataa tgtcgcccaa tttgaaatag ataccggcaa aggtcacaat
     9001 ttgcagaaaa gcaaccatga aaaatggata ggttgccgaa atattgaaaa acatcgtgga
     9061 gatgctaaat gcagtcgtaa tcaccaaagt aactcctgct cgcatcataa tcgtattgaa
     9121 caagcgaata atcgccttct tgcccaaact ctcataagtc ggcaacatgg acaataagaa
     9181 actaatcggc aaaaacatcg caaagataat aaagagaatt tgcgagaaca acataatccc
     9241 tgttaggaga aagacaaaga aggatatgcc aatattgaac aacaccaaaa agaccacggt
     9301 ccctaagcgg ttcatggtct tggtaatact gagattcgca ttgtcattat cttcaatctc
     9361 cgctttgacc gcttcttccc gatccttgcc tttattttca tctggactta cagacaggat
     9421 tttgttaaca cgatcttcgc caacttcttc tatattggaa tcatcaaatt gcaagagtaa
     9481 ccacggttgt tggacttgaa tggaaaacaa gctatctcgg attaaatcca cactgtcctt
     9541 tccttggctt tctgaattcg gcacgacaat ctcggttcca aggctaagag ccgcttcgct
     9601 gacatctgag ctaaaatcat tgatttttgt gatataggtg ggcgcatacg caatgaacga
     9661 acccgaaagt agaaaaatca tcacaaaatt aatgacagcc cggactgctt tggtagtttc
     9721 tcgttttaat aatcctgtat acgccacata aactcctaga gctaaaatca tcaataatag
     9781 aaacccaaag taaaagcccg aggtctggag tccattttcg gtaatgccgg ctaaggtttg
     9841 gatattcttc ccaatacttt cagccgtatc tgaaatgaaa tccagcttat aggcttcttg
     9901 aaccacgtag cccgtggcat ttgataagta gagactaact gtccaaataa aattagtaat
     9961 ggcataaagt ccatacatta cgctcttccc taggccatca ccccaattcc aaggcagcca
    10021 atcccacgaa ctatccacaa agaaatccaa ctgatagtta ccaagtgaat attgggaata
    10081 aagatttccg gctccaactg tatcatcaac cagacctgtt gcctcagcta ccgttccaac
    10141 taagctcaaa agtattatca gagtgaccaa actgattaaa acaattccaa agatacgaaa
    10201 aagtttcttt ttcatctcct cactccattc tctcttgaac tggcggacgg gtatcaaaag
    10261 catggaaaag ttcttcaaag actggatgaa cttggaccac acctactcga ccatacaaat
    10321 cctgcatcaa gcattgccca ttttccaaat ctcgcatgca tttttgatta ctttcatctt
    10381 ctttatctaa tccaaagaat tccaaggttt tcttgatttc atgaatatcg gtactgcgaa
    10441 atgcaaactt tagcccaata ttattcttca aacgttcatc caacaagtca tcactgtttt
    10501 gcgtgacgaa gtaaacaccg gcattcatgg cccgaccagc acgcactaat ttattcgata
    10561 acgtttttcc ttgagctact tgaagaaaac tccaagcctc atccaggtcc acaatcttga
    10621 aaatttctcg gttgctatgg ataaagtcca aggcaaaagt ggaaataaca attagcatgg
    10681 aaacactcag caattccatg gtggtatatt cttcaaaagt ggtttctgca tctggtaaaa
    10741 ccaaatccgc tacttgaatg atatttaact gcttatctaa actaatcgac tgggtaatcg
    10801 aaccatctga gaaaagcaaa tgggcaaaat cataatcggt aaaggactcg atgtgatccg
    10861 cgatattttc cgcgactggt gagtcctctt tacgcaattc aacgattact tgtaataatc
    10921 ctcgctgatc actttgggta accgttcgaa tggctttgcg taacacaggg aacttctccc
    10981 catcccgact ggaaatccct gttaaaaagg tcaggatatc aatcgccaaa ctttctgagt
    11041 ctttcttttt cttcataatc acatagggat caagtaagcc ttgattttct ggcttactgg
    11101 tcaagttaag aatattaatt tcatccgcaa tttctggcaa cgtttctttc caaccgcctc
    11161 tttctccttt tggatcgaca atcacagctt gcccaccaaa aaggacggca taatagacta
    11221 agagattgtt gctaaaagat ttccccccac ctaacgaacc aaggaaagca gatgccaaag
    11281 cattggttac tgagcctttg accccttgtg atgccaatgc cggtttcagg taaacattgc
    11341 gccccgtatc tacgttatag ccaaagtaaa tgccttctaa ttctcctagc atttgagtag
    11401 ccccaaaacc aagtcccgcc aaaaagtcag acgtaacata ttgaatatag tcattcatgt
    11461 atcgcttact tgagggtaag aattctttgt gtagacccat catatcgcca aagggccgga
    11521 ctagttttac attggtatcg tcataaaaat cgagcacttc atcacagcgg cgttttaatt
    11581 catccactga tttagcactc actcgaacca catagcttag tttatacatg gattctttcg
    11641 actgatccaa ggtggtttcc aattcatcca cggaatccaa agcatctaat acattggagt
    11701 tcgtttcatt atcagattga taggcatggt tgtctaaatc ttttaattcc tttttcttat
    11761 tacgaacggt agctagggcc tttttattcg tcacaatttc cacattcatt gaagtatcaa
    11821 tcggaaacgt aaattgttgt tgctggtaat agaaaagttc cgaggaagga aactccattt
    11881 cccctacaat ggtattaatt gttaagtacg ctacataaga ctcatgattt tcatgttcca
    11941 tccgtaagta ccgaggcttt tcttcaatca aacaacgagt gggacgtaat aagtcgtaac
    12001 gtttcaccag cgtttctgat ttcagctttt tctttggtag ctggaattca tattcttcaa
    12061 acggtgtgcc tttttcacca tagatatgtt caattaaata cgtcaagtca ctcggcgtta
    12121 ctcgtttgac tttaaatctt cgagccaatt tactttccat tagtttttca agcttggtat
    12181 agcgacgaat ttcttcattg gataaggaaa caaaatcacc catcaaatga tgattcactc
    12241 catagacaaa ttcttgaagt ccagaaaaga aagatttcct gagattcttt agattgacct
    12301 cttcatctgt cgaaatcagt ttaaagccaa taaagaagcg ataatcaatc tgactatcgc
    12361 caatcatgga tactaatgca tctgtctgta aatcaattcg ttgctttgct acttctttga
    12421 gtctaccggt aatttccttt ttcgaacgtt cttgtgtggc acgaatacta ctttctgtgg
    12481 caatttgtaa ggcatggatc ttaccttcac gattttgcgc aattaattgg cgaaagttat
    12541 catgtacttg atacttctgt tcaggtgaca aaaaggagta attgtaaggc actaattcat
    12601 agtaagcaaa acactcgcct tcttggttaa agacaagatt attttcaata tacttaatcg
    12661 ggtacataat tcacactcct taccaaggtg attg
//
LOCUS       DACDVT010000069        12527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDVT010000069 DACDVT010000000
VERSION     DACDVT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.070"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1161
                     /locus_tag="IWL52_002051"
     CDS             22..1161
                     /locus_tag="IWL52_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9778585.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1250..2215)
                     /gene="nrdF"
                     /locus_tag="IWL52_002052"
     CDS             complement(1250..2215)
                     /gene="nrdF"
                     /locus_tag="IWL52_002052"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9778586.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(2265..4424)
                     /gene="nrdE"
                     /locus_tag="IWL52_002053"
     CDS             complement(2265..4424)
                     /gene="nrdE"
                     /locus_tag="IWL52_002053"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9778587.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(4583..4807)
                     /gene="nrdH"
                     /locus_tag="IWL52_002054"
     CDS             complement(4583..4807)
                     /gene="nrdH"
                     /locus_tag="IWL52_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9778588.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            5208..5681
                     /locus_tag="IWL52_002055"
     CDS             5208..5681
                     /locus_tag="IWL52_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9778589.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            5678..7810
                     /gene="feoB"
                     /locus_tag="IWL52_002056"
     CDS             5678..7810
                     /gene="feoB"
                     /locus_tag="IWL52_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9778590.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            7812..7946
                     /locus_tag="IWL52_002057"
     CDS             7812..7946
                     /locus_tag="IWL52_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9778591.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            8040..8684
                     /locus_tag="IWL52_002058"
     CDS             8040..8684
                     /locus_tag="IWL52_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9778592.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            8871..10064
                     /locus_tag="IWL52_002059"
     CDS             8871..10064
                     /locus_tag="IWL52_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9778593.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            10057..11784
                     /locus_tag="IWL52_002060"
     CDS             10057..11784
                     /locus_tag="IWL52_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9778594.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            12178..12375
                     /locus_tag="IWL52_002061"
     CDS             12178..12375
                     /locus_tag="IWL52_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9778595.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            12377..>12527
                     /locus_tag="IWL52_002062"
     CDS             12377..>12527
                     /locus_tag="IWL52_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9778596.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPL"
BASE COUNT         3931 a         2173 c         2582 g         3841 t
ORIGIN      
        1 aaaggacctt ggaggagatg tatgcaatca cgttttaaac aatcaaaatt atttttctgg
       61 acagtggagc tagtactcac gatcatcggc gtgtacttta ttttacaaat gccgaatatt
      121 tttagtccaa tattgaaaat gctttcggca atttttttac cattgcttgt tgctggtttt
      181 atctattata tgttcgatcc aatcgttgtt tttctcgaaa agcatcggat cccgagagtg
      241 ttcggttttt tattgacgtt tttggtcgta atcattttag ttgttttaac tgtcatgaat
      301 gtagtgcctc agttaatgga acagacaatt caattgaccc agagcttgcc aatgtatgca
      361 gatgaaacaa gcaaatggtt aaatgaattg gcgcagcggg aagagtttaa aaatttcaat
      421 ttggaagaac aattagcatc agcaaattta tctcttagta atttgttcaa tatcatatta
      481 gtcagtttat ctgcaagtgt ctcaaaaatc atttcattta tgatgcaatt ttttattttg
      541 ttatttaccg ttccatttat tttgctattt atgttcaagg atgggcacaa atttattgat
      601 gcgttgtctc acttctttcc gaaagcgatt cgtaaagagt tacgccaaac ggtaaaagaa
      661 ttaaacgaaa cattatctgc ctatatcagc agtacgatct tagatgcttt gattattggt
      721 gtaatgagct ttttggcaat gagaatattc gatcagcctt atagcttgtt gcttgcggtc
      781 atttgcggaa tcacgaatat tatcccgtat gtcggccctt ttattggagc ggtaccagca
      841 atcattgtcg gtttgtttat ctctccattt caagctttgt atatggcgtt gtcgatttta
      901 gtgatccaac aattagatgg aaatttgatc aagccgttgt tatttgggaa atcaatgaat
      961 atccatcctt tgacgatcat cttggtattg attggagcag gaagtgtcgc aggaatatta
     1021 ggcatgctga tttgcatacc tgtctatgca gtgattaaga cgataatatt aaatatccgg
     1081 aaaatctatt tattgagaag aatggaacat gtttcggaaa agttagacag tccaaaagtg
     1141 ctggaagaaa caagtgaata agcataaaaa agagttcagt gtacatttaa aatatctgta
     1201 cgctaaactc tttttatttt tcgaaaaatt tttgaattaa aataaatcct tattccaatc
     1261 caatcaaata atcgtcgtct ttcatcgctt ctacatgacc taacaaatag ccgtttccga
     1321 cttgtgagaa gaagtcatgg ttgcttgtgc ctgttgaaat cccgttcatt acgattgggt
     1381 tgacatcatt ggcagtatct ggaaagagtg gatccatccc taggttcatc aatgctttgt
     1441 tggcattata gcgtaggaat gtcttgactt cttctgtcca gccgattgga tcgtataatt
     1501 cttctgtata acgttcttcg ttttcgtaaa gctcataaag aagattgtac atccagtctt
     1561 ttaacgtttc ttgttctgct tcaggtaact cgttgaatcc taattggaat ttgtaaccga
     1621 tgtaggttcc atgtacagat tcatctcgga tgattagttt gatgatctct gctacgttag
     1681 ccaatttgtt gtttcctaaa taataaagag gagtataaaa acctgaatag aacaagaatg
     1741 tttctaaaaa gacgctggca attttctttt ctagcggtgt accgtgtttg tagatttcat
     1801 tgatacgctc agcttttttt tgcagataag gattcgtatt tgtccagtca aaaatttctt
     1861 cgatttcttt tttcgtattc agtgtactaa aaatagaaga ataacttttg gcatgaacgg
     1921 attccatgaa ttgaatattg ttcaaaacgg cttcttcatg gggagtgcgg acatctttac
     1981 gcaactgatc catgccactt tcagattgga cagtatctag cagtgtcaaa ccgccaaata
     2041 catgtcctac tgtatctttt tccaattgag acaatgtacg ccaatcatct aggtcatttg
     2101 aaagtgggat acgtgtatct agccaaaatt gctctgtcag cttctcccaa gtggatttat
     2161 caatgatatc ttcaatttca ttccagttta ttgcttcata atatgtttct gacatgagaa
     2221 tgctccttta attaaaatat gaataagcga atctatctat gatattagat cacgcagctt
     2281 tcacattgat tgcttccgat ttcttctgca tcatcagtaa aggtacgtac gtaataaatg
     2341 gatttgattc ctttgtggaa agcgtaatgt cttaaaatat tcaaatcacg tgtcgtttgc
     2401 ttcgttgttg tcttccattc ataaatgcct tctgggattt cagaacgcat gaataaagtc
     2461 aaactcatgc cttgatcgac gtgttgttgt gccgttgcat aaacatcgat tactttacgc
     2521 atgtccatat cataagcaga agtgtaatac ggaatcgtct cattggatag ataaggcgct
     2581 gggtaataaa ttttaccgat tttcttctct tggcgttctt cgatcatgcg agtgatagga
     2641 tgaatacttg cactcgtatc gttgatatat gaaattgagc catttggtgc tactgccaag
     2701 cgattttgat gatacagtcc atctttcatt actgcttgtt tcaaagcatt ccaatcttca
     2761 gcagttggga taaaaatgcc ttcaaacaat gctttgactt tttctgattt tggctggaat
     2821 tctccagtta cgtatttttc gaaataagtg ccgtcagcat aagctgattt ttcaaaatta
     2881 tggaaaactt gttttctttc acgagcgata ttgttgcttt caacaagtgt ccaataattc
     2941 aacagcatga agtaaatatt tgtaaagtct aatgaatcct ctgagccata ttccatttgt
     3001 tcttttgcaa gataagtgtg taagcccatg gcacctaatc caatcgtatg attcagctta
     3061 tttccgtttt ggatcggtgg tacgacatcg atatcagatg catctgtaat aaatgtcaat
     3121 gcacgagtca tcgtacgcac agattgacca aagttagggc tttgcatcaa attcacgata
     3181 tttgtcgagc ctaagttaca tgagatatct gttcctaata cttcatattc ctgtttgccg
     3241 ttaatcacag aaggcgtttg aacttgtaaa atctctgaac ataaattact catgatgatc
     3301 ttgccatcga ttggattctc gcggttcgct gtatcaatat tgattacgta aggatagcca
     3361 gattcttgtt gtaattttga gatttcattt tcaagatcac gcgctttgat tttttgtttg
     3421 cgaatgtttg gatttgcaac tagatgatca tactcagctg taatatttac ataagagaat
     3481 gggacaccat attctttttc gacactatat ggactaaata aatacatatc ttcgttttta
     3541 cgtgtcaatt cataaaattt atcaggtacg atcacgccaa gagaaagagt ttttacgcga
     3601 atcttttcat ctgcattttc tttttttgct gaaaggaaag caataatgtc tggatggaaa
     3661 acgttcaaat aaaccacgcc agctccttga cgctgaccta attgattaga atagctgaaa
     3721 ctatcttcga ataatttcat gactggaacg acaccacttg cagcaccctc atatcctttg
     3781 ataggagcgc cggcttcacg aagatttgat aaagtaatcc ctacgccgcc accaatacgg
     3841 gacaattgca aagcagagtt gattgagcgc ccgatagagt tcatatcgtc agtgacttga
     3901 gtcaagaaac aagacaccaa ttcgccccga cgtttacgtc cagcattcaa gaaagaaggt
     3961 gtagctggct gatatcgttg atggatcatt tcgtctgcaa gagcaagtgc taattcttca
     4021 tccccgttcg caaagtaaag cgcattgaag gctactcgat cttcataagt ttccagataa
     4081 gcggaaccgt cgttggtttt caacgcatat tgagaataaa atttgtaagc agccataaaa
     4141 gatttgaaag aaaaatgttg ctcgtccaaa aattgatata gcttttcaat aaatgatggt
     4201 gaatacaatt gaatgaattc tgcttctata tattcttgat cgatcaagta attgattttt
     4261 tccataatag atggaaaagt catcgtattt ggtttgacat tttcctcaaa aaaggcaact
     4321 aaagcttctt tatccttatt caaagggatt tgaccattga ctggccgatt gatttcatta
     4381 ttcagcttga aataactgac atcttgtaaa tttttaagac tcatacgtac gcccaacctt
     4441 catttcgtat ttgatgatgc ttgattgcaa atcattgcat tgtttttaag ctttaaaaaa
     4501 ggatttggaa gtttgcgaca aaaactggtg ctgtctcaag cccttcaaat ccatgtatca
     4561 cgttttgatt cttttagtca tgttagctgg caagtttacg caactgatca ggacggaatc
     4621 cgataactgt ttctgcatca gaagtaatga caggaacact ttggaatcct tgtgctttca
     4681 accagtctac tgcttcaggc tgttcatcga tattgacttc ttcaaaagca atttgatttt
     4741 ctgttaaaaa ccgtttgacc atcttacatt gcatacaatt atttttagaa aagattttta
     4801 cgttcatggt gagtgcctcc tctaacacat tcttattatc tataatagat agtataaaac
     4861 tcttagtaaa aaagtcaaac aacaaacact acatattgtg ttgtggaaac tgtggaatac
     4921 taagttttgt gtttttgaaa aaagagctga aaacaagtat cgtgtgggaa aatagcaaat
     4981 ttaatagaga aaaaattgtg gaaaactatg aagaaatatt tttttctcat ttttatctat
     5041 caatactaaa gattcgctca taatatcaca agtataattg acattctcaa ttaccgagag
     5101 tattctatgt gagaatgatt ctcatttatt tgcgtaacac gtaagaaaat tagaacatat
     5161 aatgaatgat tttaatacat gatttatttc acaagagagg aatcaatatg cagaccttag
     5221 cgcaggcaaa gcagaatttc gtttatcaag tcgaacgaat cgaaacagaa gaaaaaacaa
     5281 aacgccattt gcaaaatatc gggatcgttg ccggaagtaa aatcgtcgtc gtcaatttat
     5341 caggcaacaa tgccatttta ctggtaaaaa gtcatcgagt ggcagtaagt cgggaaatac
     5401 taggtcaaat ctttgtcaat aaaaagactg cttcctctga aagctggact tctcttgatc
     5461 aattaaaagc aggagagact gggatcgtcg tagcaatcca cggacaaggc gcagtgaagc
     5521 gacggttaat ggatatggga ttgacgcgaa atgtaaaaat agttatccga aaactagccc
     5581 ctctgggaga tccaatcgaa ctgacagtgc gagggtatga actgacgtta cgaaagtcag
     5641 aggccgaact aatcttgata caaagggagg aaaaagaatg aatatcgcgt tagtcggaaa
     5701 tccaaacagc gggaaaacta gcttgttcaa tctcttgact ggttcgaatc aatatgtagg
     5761 gaattggccg ggcgtaacag tagaaaaaaa agaaggatcg ttgaaaaaaa atccgaacat
     5821 tcatattcag gacctacccg gcatctattc tttgtctcct tatacaccag aagaagtgat
     5881 tgcaagagat tatttggttc aggaacagcc tgaactgatc atcaacatta ttgatgctac
     5941 caatttagaa cgaaatcttt atttaactac gcagttattg gatttagaca ttcctatgat
     6001 cattggtctg aatatgatgg atgtcatcaa aaaagaagga aaaaaaatca atctagaaaa
     6061 gctttcctat ggtcttggtg taccagtttt accaatcagc gtattgaaaa agcaaggaat
     6121 cgaaaaacta atcgaaaaga ttcaggagtc tgcaaaaaag cggccttcta ttcctgttcc
     6181 ctgtacatat gatccacgtt tagaagcagc tttacatgaa attgaagatg tactaggaga
     6241 acaaagcggc aagaaacgct ggtatgcaat gaaatatttt gaacgagatc agaaggtaag
     6301 agaggattac gagatagcag tttctcagca aaaagagatc gagcaactga tccagttaac
     6361 agagaaacta ttggacgacg atagtgagac gattctggtc aatgaacgtt atgaatttat
     6421 tacccaacta tgtacactct gtgttgtgtc aaatgattct ttccaattat cgatgagtga
     6481 caaaatcgat cagattgcaa cgaatcgttg gttagccttg ccgatcttcg catttgtgat
     6541 gtggctgatc tattatctgg ctattcaaac agtaggaaca atggggacag attggatcaa
     6601 tgacacattg tttggtacat ggttaccaga acatgttggc agattgttag ctgaatggca
     6661 agtagcggga tggatgcagg agctgatctt gaacggcatc atcgcaggcg tgggagcagt
     6721 gttaggattt ctaccgcaat tgattatatt gtttttatgc ttgagctttc tggaagactg
     6781 cggatatatg gcgaggatcg cttttgtcat ggatcgactt ttcaggaaat tcggtctttc
     6841 aggaaaatcc ttcattccta tgctgattgc tacaggatgt ggagtgccag gcgtcatggc
     6901 cagtcgcacg attgaaaacg agcaagatcg tcgtttgacg atcatggtca caaccttcat
     6961 gccttgttct gcaaagttgc cgattattgc cttagtagca ggggcctttt ttcctcatca
     7021 atcttgggta gctccttctg cctattttct tggtatgact gcaattattt tctctggaat
     7081 cagcttgaaa aaaacacgtt tgtttgctgg agatacagct ccttttatca tggaattgcc
     7141 cgcatatcat tttcctcaat ggtcagcggt cctaagacaa acctatgacc gcagcaagtc
     7201 ttttgttaaa aaagcaggaa cgattatctt tgtttctagt atcgtgatct ggttcagctc
     7261 ttcttataat ttttatgctc aacctgtttc tgaagaccaa agtatcttag cctttttcgg
     7321 gcgaatcctg gcagttgttt ttcagccgct tggttgggga aactggcaag gaacagtcgc
     7381 agcgattaca ggattggtcg ctaaagaaaa tattattggc acatttggta ttttgtttgg
     7441 tcatgcggaa gtatccgaaa atggcagaga aatctggcaa gtgctacaac acacatatac
     7501 tcccgcagca gcttattcat tgttagcctt caatttactt tgtgcgcctt gttttgcggc
     7561 aattggggct atccatcggg aaatgaatgc caagaaatgg acttggatcg ccataggcta
     7621 tcaatgcggt ttagcttatt tagttagttt tgtgatttat cagttgggac acttattgga
     7681 gggtggacag gtagcgttag ggacgtatct tgctatttta ctgatgatag gactcgttta
     7741 tgttttgttc cgtcagccaa aatccaagaa ccagttttat actatattgt cattagaagg
     7801 agaggaatag gatggcgaca ttcttattaa gtgtgttaat ctttggaagt gcaggcgcaa
     7861 ttgtctatac tcgtttaaaa tccggtaaga attgtgatga ctgtcaaaca gcttgcccag
     7921 taaaaaagga acagatcaag cgatgatcag tcgttgcaag aggctattct cccctttttt
     7981 gcttattttg ctagagcata gtaagatgag ggaaagtaaa aaaagaaaga ggaaagaaaa
     8041 tgtctggaaa gaaaaaaatc gtccgcccgc gctaccagca gattgctgtt gatttagccg
     8101 aacgtatcgt tgaaaaccga tataaagtgg gtgagaagat ccatgcccgc tcaacacttg
     8161 ccagtacgta caatgcttct cctgagacta cccgcaaggc tatcaatgtg ctggtagatc
     8221 ttggaatcat ggaagtacgg catggaagtg gggcatttgt tgcttcgaag gaaaaagcaa
     8281 aagatttcct attgcaatat caagatgtac aatccatcca agatacaaaa gcagaaatca
     8341 tggcaagtgt ggagaagcaa caagatgagc tgaatcattt ttctcaatta ttggatcagc
     8401 tggtcaatca aacgaagcga atacataaga tgaatccttt acttccgttt gaattatctt
     8461 tatcagaaaa agcaatgcat ctggaccgtt caattagcga gttgaatatc tggcaggcga
     8521 ctggcgccac agttatcgca atttctcata atgaagaact gcttgtttca cctggcccct
     8581 atgctaaatt tacggcaggg gacatcgttc attttattgg aaatgaatat acactacagc
     8641 gtatgacaaa ctttttttac ccggagcatc agcttgcaga atgattgtgt gaaatattaa
     8701 aaaaatatta acttggctaa accttttatt tacaaagatt tagccaagtt tttttcagtt
     8761 tggacaagca aatttctttc atttttgaca aagtgactac atgatgttaa catagtgtgg
     8821 tcactttttt cagaaagaaa aatgaattta cataaaaaag gagaatttgt ttggtaaaag
     8881 tagaagtaaa acatttaaca aagatatttg gtaaaaagac acaagccgct ttagatatga
     8941 tgaatgatca ccagccgaaa acggagattt taaagaaaac cggtgcaacg gttggtgtgt
     9001 atgacgtgaa ttttgacgta aaagaaggag aaatcttcgt gatcatgggg ctttccggga
     9061 gtgggaaatc cacattgatc cgtctattga atcgtctgat tgagccaact tcgggcagta
     9121 tctatattga tggagaagat gtatcgaagt taagcaaaga agaattgcga gaagtccgtc
     9181 gtcataaaat caatatggtt ttccaaaact tcggtctgtt tccacatcga acgatcttgg
     9241 aaaatacaga atacgggtta gaagtccgtg gtgtacctaa agaagaacga caagaaaaag
     9301 cagaaaaagc attagaaaat tctagtttgc tttcatttaa agaccaatat cctagtcagt
     9361 tatctggtgg gatgcaacaa cgggttggcc tagccagagc tttagcaaat gatcctgaga
     9421 tcttgctgat ggacgaagcg ttctcggctc ttgatccgct gatccggcga gaaatgcaag
     9481 acgaattact ggacctgcaa gccaatgttc aaaaaacgat tattttcatt acccatgatt
     9541 tgaatgaagc tttacggatt ggggacagaa tcgccttgat gaaagacggc gaaatcatgc
     9601 agatcggaac aggtgaagaa atattgacca atccagcaaa tgattatgta cgtgaatttg
     9661 tggaagaagt cgatcgttcc aaagtgctta cggctcaaaa catcatggta cctgcattga
     9721 cgacgaatat cgaatctgat ggaccaaacg ttgcattgac aaggatgcgt aatgaagaag
     9781 tcagcatgct gatggctgtt gacagaaaga gacacctgaa agggatcatc actgcagatc
     9841 aagcgttaga agctaggaag caaaaacgtc cattgatcga cttcctagat gaaaatgtga
     9901 cggtgattgg gaaagacatg gtggtaagtg atattttcaa tattatttat gactcaccga
     9961 ctccacttgc agttgtagag gatggaaaat taaaaggtgt cgtcatccga ggtagtgtga
    10021 ttgaagcatt agcagaaaca agcgaggtga gtgaacatga ataatctatt gaactatcaa
    10081 ttaccagtgg caaactgggt agaaaatatc acagaatggt ttacgacaac tttttctggc
    10141 ctattttcat ttttacaaac gattggacag gcagtgatgt cggggattac aaatttactg
    10201 ttagtgattc cagctccttt gtttatcttg ttattgacgg tagcagcttt ctttatttct
    10261 aagaaacgtc caggacttac gttgttcaca ttaatcggtc tatggtttat ttataaccaa
    10321 ggtctatgga atgatttgat gaatactgtc acacttgttc ttttatctag tgtgatctcg
    10381 atcatcattg gggtaccatt agggatcttg atggcaaaaa gcagtaaagc ccaaagtatt
    10441 atcaaaccga tccttgactt tatgcagaca atgccaggat tcgtttattt gattccagca
    10501 gttgcatttt tcggtatcgg gatggttcca ggggtatttg cctcagtcat ctttgctctg
    10561 ccaccaaccg ttcgttttac gaatttaggg attcgacaag ttcctaaaga attagtagaa
    10621 gcttctgatt cattcgggag cacaagtcgt caaaaactat tcaaactaga attgccttta
    10681 gctaaaagta cgatcatggc agggatcaat caaacaacaa tgctgtcact ttcaatggtc
    10741 gttattgcct ctatgatcgg tgcacccggc ttaggacgcg gcgtattatc agcattgcaa
    10801 agagcacaag tcgggaatgg atttgtcaac ggggttgcat tagttatctt agcaatcatc
    10861 attgaccgtt ttacacagca tttgaaccag ccaaacaata aaaaagctgc aggagcaaca
    10921 acacagaaaa gtaaaaaacg tcaaggactg atcattggtg ctgtagtagt agtgatctta
    10981 ggtgcgattg gtatagggag tttttcatct gccaaagaaa caaaacgtat caacttatct
    11041 tatgttgaat gggatacaga agttgcttca acaaatgtag tcggtgaagt attgaaacaa
    11101 atgggctatg atgtaacgat gacgccatta gataattcaa tcatgtggaa atctgtttca
    11161 aatggcgagt cagatgccat ggtttcagct tggctaccta aaactcatgg ttcacaatat
    11221 gcacaatata aagatcaagt cgaagaccta ggtgccaatt tgacaggagc aaaagttggt
    11281 ttagcagtgc cagcctacat ggatgtaaat tcgatcgatg agttaaccga tcaggcaggt
    11341 aagaaaatca taggaatcga gccaggagct ggtgtagtta cggctgctga aaacacgatc
    11401 caaaaatatg acaacttgaa agactggaaa gtagagacat cttcttcagg agcaatgacc
    11461 gttgcactag gtcaagcgat caaaaaacac gaaccaattg tagttacagg atggacacct
    11521 cattggatgt ttgccaaata tgatctgaaa tacttagaag atcctgaaaa cggaatggga
    11581 agcgaagagc agatccatac gatggttcga aaaggattga aagaagacca gccagaagct
    11641 tacaaagtat tagacaattt ccattggtca gaaaaagata tggaaaaagt tatgttggaa
    11701 atcaacaatg gaaaagatcc acagcaagca gcaaaagact ggatcaaaga gaaccaagaa
    11761 ttagtcgaaa gctggaaaaa ataaaaagac agacacgaaa taaatggagc ttccaaaatt
    11821 ggatcaaata tccaaatttg gaagctccat gttcgtctct atcttttttg tctataaaaa
    11881 aagagaatgc gtagctcatc ttcgaaacgt tcaaagacgc cgacaaacta ttatgaattt
    11941 atgggtgcga gtcaaagaga tcgatcgtag gtatgaaaga ctaatgtgat taaagtttat
    12001 tatgtacgaa gtgcattctc aaaaaatatt aaagaatata catagtttat atacttaaaa
    12061 gtgattaaat tcttattcag gaattatttt gctacgtttc aagaaggaat tgttttttga
    12121 aatttaatga atgttagaat aatagtctaa aaacattaat ttaggggtga ttagattatg
    12181 aaacatttaa aaattttgtc tattaaagag acacaactta tctatggggg taccactcat
    12241 agtggaaaat attatggaaa tggagtgtat tgcactaaaa ataaatgtac ggtcgattgg
    12301 gccaaggcaa ctacttgtat tgcaggaatg tctataggtg gttttttagg tggagcaatt
    12361 ccagggaagt gctaaaatga aaaaaaatgc taagcaaatt gttcatgaat tatataatga
    12421 tatatctata agtaaagatc ctaaatattc tgatattctt gaggttttac aaaaggtata
    12481 tttaaaatta gaaaaacaaa aatatgaatt agatcccagt cctttaa
//
LOCUS       DACDVT010000070        12236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDVT010000070 DACDVT010000000
VERSION     DACDVT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.071"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            362..1333
                     /locus_tag="IWL52_002063"
     CDS             362..1333
                     /locus_tag="IWL52_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9778597.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1411..1647)
                     /locus_tag="IWL52_002064"
     CDS             complement(1411..1647)
                     /locus_tag="IWL52_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9778598.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1616..1747)
                     /locus_tag="IWL52_002065"
     CDS             complement(1616..1747)
                     /locus_tag="IWL52_002065"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9778599.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1823..2206)
                     /locus_tag="IWL52_002066"
     CDS             complement(1823..2206)
                     /locus_tag="IWL52_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9778600.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2239..3024)
                     /locus_tag="IWL52_002067"
     CDS             complement(2239..3024)
                     /locus_tag="IWL52_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9778601.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3202..4302
                     /locus_tag="IWL52_002068"
     CDS             3202..4302
                     /locus_tag="IWL52_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9778602.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4292..5116
                     /locus_tag="IWL52_002069"
     CDS             4292..5116
                     /locus_tag="IWL52_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9778603.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5122..5928
                     /locus_tag="IWL52_002070"
     CDS             5122..5928
                     /locus_tag="IWL52_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9778604.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5925..6755
                     /locus_tag="IWL52_002071"
     CDS             5925..6755
                     /locus_tag="IWL52_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778605.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6762..7793
                     /locus_tag="IWL52_002072"
     CDS             6762..7793
                     /locus_tag="IWL52_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778606.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7900..9173)
                     /locus_tag="IWL52_002073"
                     /pseudo
     CDS             complement(7900..9173)
                     /locus_tag="IWL52_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9450..9659
                     /locus_tag="IWL52_002074"
     CDS             9450..9659
                     /locus_tag="IWL52_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778607.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9709..10425
                     /locus_tag="IWL52_002075"
     CDS             9709..10425
                     /locus_tag="IWL52_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9778608.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10333..11266)
                     /locus_tag="IWL52_002076"
                     /pseudo
     CDS             complement(10333..11266)
                     /locus_tag="IWL52_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            11554..12207
                     /locus_tag="IWL52_002077"
     CDS             11554..12207
                     /locus_tag="IWL52_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9778609.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3522 a         2269 c         2794 g         3651 t
ORIGIN      
        1 tatatttttt actctcagcg tttttgactc cgtgtctgac tccgttttat tgattttgaa
       61 tgttattcaa tgaccattta acaggtcaaa tttgatgttt ttgattccgt atgacgccgt
      121 ttgatatcta gtgatatagt gatttgagtc tacatcgaat aagggaataa accaataaaa
      181 aatgatttag aatattaaaa taacaaaaaa gaggactacc tgtattagtc ctcttttttg
      241 accagatttt gaccagtcta ttttacatcc tagtatattt atatgagcta attttatgat
      301 taaatggtaa gtttttcctt gtgtgtttta gtttaaacta gtgtgtaatt caaaaatcta
      361 tatggaagga acagtattaa gaatagaaca gggttctcta catgatggtg ctggtttacg
      421 gaccgttgtt tatttaaagg gctgcccatt acgctgtgcc tggtgttcga ttcccgaatc
      481 acaaagcaag cagattgaaa aaggatttgg ccagaccatg accgctgaag aggtaatgga
      541 tgagattgag aaggatgctg ttttttactt tcactcggat ggcggagtga ccattagcgg
      601 aggcgaggca ctggttcagg cggattttgc taaagaaatt ttgcaaaaat cgaaatacat
      661 tggaatcaat accgtactgg aaaccagctt ttgtggtgct tataatgaaa tccaaaaggt
      721 agctccctat gtggatacat tgtttgtgga tgtaaaaatg tttaccagca agctccacaa
      781 acaatggacc ggcttagata accaacaaat tttgaagaat attcgtcgct ttttaattga
      841 gtatcctaac tgcgaagtgc gaattcgagt tcctgtggtt ccaggtatca atatgaacct
      901 gaccgagctg ctgacgattg cttgttttgt ggcagatctt gatcggttcg taccgttaga
      961 attactaccg taccactgct atggtatgca cggttaccaa gcattgggac tggagtatcc
     1021 gttggcagac acgccagcgc cttcacctaa agaaatgttt gcattggcag atcaactggc
     1081 tcgcacggtt cctcatttgc tggtcacagc tttaaccaaa acatttattt atttattgaa
     1141 tccaagcttt atcttatgga taaatgaaag acactcacta gctgcagcca gctcaagaat
     1201 agagcttttg ctgaagtcag tgagtgtctt tttacgatgt tttttcgaag ttaatttgtc
     1261 gaatgaacac tggctcaatc ggtacatctt cactgtttgt cggctgagct gcgatcgctg
     1321 ctacgatttc taacccttgg tatacctgtc caaaaacagt ataacgacca tccagctccg
     1381 gtagtcctcc aagctccaag tatgcttgct ttacattagc aggtcagcct gcgcctttgt
     1441 gttttgcaca ataaaaaact ggttgccgtt ggtgtggggg ccgtggtccc ctaaggccaa
     1501 ggcgccatta aaatgataaa gtccgcgatg cacttcatca gcgaaaaagc cctcatagct
     1561 ggaaacagct tcatcaccag aaccatccaa agcgccacct tgaataacaa attccttaat
     1621 gacacgagcc gaaaggtgtg ttgtcataaa cgccttgctc agctagggag cgccagttgg
     1681 ctacagccag gggagcttgg tcaggaaata agcggagacg aatttcacca gcatcggtga
     1741 ccagcacaca aacttcttcc tgctgcggtt tttctaattg aaagagagga aggtttgcaa
     1801 ctgaaacgcc ttgatcctgc catcaaatat cgaaaatccg ccactcgacc ttcgcctgat
     1861 caccaaggcg agccattccc aataaaacgg tataggcggt ttcatccgct tgaaaggtca
     1921 attgcagctg tcctcgtaaa tcgttgtagt catctggggc ggctacttga taaaaaagat
     1981 gaaactgcgc atgcaaatat tccaaaggct gatctttaaa ggctgcaaaa taagcctgaa
     2041 ctttttcccg ctgcaagctg tcagccacca aaaaattttc cagttgttgt tttggaacgg
     2101 ctgctaacaa ggatcggcaa accgcctcaa ttttcaacgc atcccaatgt cgcgcaatta
     2161 aataatggat gggtgcctct tgattgtagt cccacaggtt gctcattttt ttcaccactt
     2221 tcttctatta tataggaatc acaaaatcgg cagcggcgtc aggcggcgtt cttttccttg
     2281 aatgactagc aacttttcta cctgacagtc tgccaacagc tgactcattt ttttaaagcc
     2341 cagctgataa tcttcggcaa catgagcatc cgagcctaag gtaaagagct ttccgccgac
     2401 actttgatac aaaggaacgg cataacggta aaggtcttcg ttgccgtatt tcaagaagct
     2461 cttggcattt aactccatcg ccaattctcg ttcaacaacc tttttgaaaa tcgttagcag
     2521 ctgagcctca aaatgctccg cgaattcttc cgctgtgaag gtgaagcggc gtaatccata
     2581 atcaaagtga gtcaaaatat ctccttctgg aaaatccgtc aagacctgca acatctgatc
     2641 aaaatattcc ttggtagttt gaatgggatc tttggttaaa accacatcgt ccatataatc
     2701 aaagcggcca ttttgatgaa tacttaccag ctttaaatca taaggatgct gagcgaggta
     2761 atccacgatc cgctcttctt gtcctggaac aacgccaatc tcgatacctc gcagaaattt
     2821 ggtggaatag gtttctttta aagagtccag tttgtttata taggctggat aatccggaat
     2881 gtcatctttt ccggtagatg gattgtttaa atcaaagtga tccgtagaaa cgaaaaactc
     2941 cggctgatag gctaaatagt tttcaaattt ttcctctgaa tcaaaggaaa aatacgtgtg
     3001 gagatgctga tcataatact gcatagggag cctccttttt tctactatca taccgcaaaa
     3061 gctttagaag gttaacggga attcgtcgaa atttacaaac catatacaac ctttacattg
     3121 ttcatgaaac cttttcaaga aactgattct ctcattacaa agctttgtta gaatggaatt
     3181 atcgaaaggg gagaagaatg aatgaaaatg aatgaaaaaa ttcgtaacat gactatcggt
     3241 ttattttgtg cagcaacagt aagtatcctg ctgacaggct gcggctcagg tgaagcaacg
     3301 aagcaatcgg ctgcggcaga cgactcttta gatacgatta tagaaaaagc caaggaagaa
     3361 ggagagattg ccagcttggg aatgccggat acttgggcaa attgggtagg cacttggaac
     3421 gaccttgaaa aagaatacgg cttaagtcac gtggatacgg atatgtccag tgcagaagaa
     3481 ttggctaagt ttgaatcaga aggaaaaaat ggaaccgccg atattggtga tgtcggtatc
     3541 agcttcggtc cgttggctga aagcaaagag ttaaccttac cttataagac caactattgg
     3601 gaggaaattc cggattgggc aaaggatgat gatggggatt ggttgttaag ctatacggga
     3661 accatttcct ttatcaccga taaagagaat gtgaagcaag caccgactag ctggaaggag
     3721 ctgcaagagg gtgactatca agttagcgtg ggggatgtga cgattgccaa ccaagcacaa
     3781 tttgctgtat tggctgcggc gattgccaac ggaggtgacg aaaaggatat tcagccaggt
     3841 ctgaattact acaaaaaaat ggcagaaaaa ggccgtttgt ccaccgttga tgccagcgta
     3901 gcaaatcttg aaaaaggtga aatcgatgtg gcagttgttt gggacttcaa tggactaaat
     3961 tatcgtgacc aaattgactc cgagcgtttt gaggtaactg tgccaacaga cggctccatc
     4021 attagcgggt atacaacctt aatcaataaa aatgcccctc atcccaatgc ggcaaagcta
     4081 gcacgggaat atattttatc tgatgaaggg caaatcaatt tggcaaaggg ctatgcgcga
     4141 cctattcgca aggtagagct gcctaaagaa gtagctgaaa aattactgcc ggaggatgct
     4201 tatgaagcgg cggtgccagt tgaagacaat gaagcttgga acaagacctc tgtagagctt
     4261 ccagagcttt ggcaggcgga ggtactgacc tatgcaaagt agacttgaca aactgattgt
     4321 agttatacta gacggctgtc gctacgacac agccgtcgcc cagcttgggt tcatgaatca
     4381 tttagttgag caccagcaag caagcttatt gaaggtaaaa agtgagctgc ccagcaattc
     4441 acgtccgtta tacgaagtac tgatgaccgg cgttccagcc tatgaaaatg gcatctatag
     4501 taattaccat gctcagcgat ccaaagagtg gtcgctgttt gaattagtta aggaagctgg
     4561 cggaaaaaca ggagccgccg cttaccactg gtacagcgag ttgtacaatc aagcccccta
     4621 taatcgtttg gttgatcgca tccagcatca ggaggagcga ctgattcagc atggcatctt
     4681 ttatagtgag gacacttacc ctgattcgca tttatttgcg gatgcccatt atttagtgaa
     4741 tcagtatcag ccagactttt tggtcgtaca ttcgatgaat attgatgatg tcggtcacaa
     4801 atttaccgcc gattctcatg aatatgcagc agcggttaat caggcggaca gattattggc
     4861 tatttacctt cctgaatggc tgcaaaaagg ttatcaagtc gtggtgactg cggatcacgg
     4921 gatggatgcg cacggactgc atggcggcag cttggctgca catcgtgaag ttccttttta
     4981 tttgatttca aataaacgcc aaaagctgaa ggaacaaact gtaccacagc tgctggcggc
     5041 tccattgttt tgctggttat taggaattgc accgtcagag aaaatgcagg acattcaaga
     5101 attgatgagg ggatgaaaga gatgaaaaaa cgcttggttc tggttcctgc ctcctttatc
     5161 ttctgtgcat ttttactgct gccgctgctt tttatggttc tgtgcagctt gcacagtgat
     5221 ttaactaacc agtggacatt ggccaattac agtgatattt tcagcaacaa atattacaca
     5281 caggcctttg ttaacagcgt actgattgct gtcagctcca gtgtgattgg gttaatcggg
     5341 acgtttattt tatgtttatg cttaatgaat ctatcgacta aattgcagga gaaaatcacc
     5401 tttgtttcca atttggcagc taattttgct gggattccgc tggcattctc ttttattatt
     5461 ttgttgggga atgtaggtgt gctgaaaatg attttgccat ttctagcgga cttcaatttg
     5521 tattcttggt ggggattgac catcacgtat acctatttcc aaattccgtt aggggtcttg
     5581 tttttgtatc ccagcatgaa ggaagtgaaa aaggaatggc tggagtccgc acaattgctg
     5641 ggcgcttcat cagcgtatgc ttggttcaag gtggctttgc cttttttgcg accatcgatt
     5701 gccagcacct ttgtcatctt atttgctaat ggcatgggga cctatgaaac agcgtatgcc
     5761 ctgaccagca gtaatgtaaa tcttttaacg attcgaattg cggctttagt gtcgggagat
     5821 gtgttcgcca agcctaattt aggcagtgcg ttggcagtcg ctttaggtgt cctgctggta
     5881 acggcaatgt tgattattca aaagaaaggc aaggtggtgc agacgtgaaa aagttaataa
     5941 aaccgatttt gttctgcttt gtcctgtatt acttgctgcc gattattgga acgattttgt
     6001 atgccagctc cactaaatgg tcaaaatcgc ttttacctag tgattttacc ttgcaatggt
     6061 tccaacagct gctgacggac agggaattta ttgctgcggt gggtcgctca cttttattag
     6121 caggtgtggt cttggtgacg attctgttgt tgatgattcc caccattatt tggattcatt
     6181 tgtatttccc tcgtttgaat cgttggctgg aaaagctgct cctgctgccg tatgctttac
     6241 caggagtgat tttggtaacg gctttgcttc gaacgtatgc tgaaactggt atcccaatgt
     6301 ttgtagtgtt ggtaggtgca ttgtttatta cggcgttgcc gattgtctat cttagtctta
     6361 acaaccagat gcgcttaatt aatctgaagg aattagtgga tgcagcagaa accttgggcg
     6421 ccccgatgtc tacgattatt atccaggtgc tgtttccaaa catccgtatc ggtgtgaccc
     6481 tagtttctct gatgattttt tcttcggttt ttggggagta catgctgacg aacctgttga
     6541 ttggcggtcg atttcagacg gtacgcatct atatgctgcg gcggatgaac gaaaacggcc
     6601 atttggctag cgctgtgatg gttttgtatt tgctgtttat ggtgggaatt gctttaacta
     6661 cctttatgct gacttatcga caaaagcaac agctgtccct taagcagacg agggaagaga
     6721 agcagtcgaa aaagaagccc ttaaaggagt tgtgacggaa aatgttttta gaaatacatg
     6781 atctcaatct gttttttgat gaaaaacaga ttttagctat tgatgaaatt cagattgaaa
     6841 agggtgagct ggttacttta ctaggtccca gcgggtgcgg aaagtctacg ttgctgcgct
     6901 gtatcacggg attggaacaa ccaaagagcg gtgagattgt gctggacaat gagaatattg
     6961 ccgataagcc tacgaaggat cgcaatatcg gctttgtttt tcaacagtat gcattgtttc
     7021 caactatgac tgtttttgaa aatgtagcct ttggtttgaa ggtaaagaag ctgagtcctg
     7081 aggtgattca ggaaaaggtt tttgagatgc tatcattggt ggatatgacg gagcaggcag
     7141 ataaaaatgt ccagtttctt tctggcggtc aacgacaacg ggtggcactg gctcgctcat
     7201 tggtcactga accgaaagtc ctgctgctgg atgagccatt aagtgctttg gatgcccgaa
     7261 ttagaaaaca gctgcagcgg gatttgcgcg ctatccagca atcgctaggg atgacgatga
     7321 tttttgtgac tcatgaccaa gaagaggcga tgcgaatcag tgatcggatt tttgtgatgg
     7381 aagctgggcg ggtggctcag gtttccacct cgaaggaatt gtatcagaat cctcagagcc
     7441 gatttgttgc ggaatttatc ggcaactata atcgtttcga atggtatgag ctgaatcagt
     7501 atacccacga gttttccgct aagggcaatc gctgcattta ttatttaaga ccggagctga
     7561 ttcaatttga accggttcct cagggggtta agattccggt tctatggaag gaatcattta
     7621 ttttaggcaa cattcaacgg tatgtttttc aaacgctgga gggcaaagaa attttagtgg
     7681 atcggttgaa cgatcgggaa gagaaaatta gcgatgcctt atacattcag ctgaaagata
     7741 ttctagcaat ccctgtggaa attgatactc agcattttgc acaacaaagc tagtgtatag
     7801 tcggcaaaca tttctggctc tttgtcaaat aggactgata gagtgatata agaacatcta
     7861 aggtagaatt gattttctgc tttagatgtt tttttatgtt taataagtga tcagatgatt
     7921 gattaagaaa attctggttc tcatcctaac ccgtaagcta ttcgtttcaa tgctttgatg
     7981 tgggtatgca gattttctat tttgccattt gagtaaggca actctaaggc taaacgaatg
     8041 ccggattcat agtttataaa cgcttttttt atatgatgca gttctcttgg aaccgacttg
     8101 ggcattgttt ttacaagctg aaagaagatg tccgcttctt tgtcataaaa agcttgaaga
     8161 atttcttgat agtaagcata agctattcgt agttcatcag aagtagaaag tagacgttct
     8221 acgacttcac tttcggttaa ataggcccaa ccaaaattac gccattttgt ccatttatca
     8281 taaacaagcg tgtcctgtcg tttgatcaga agtcgagata aagccttcac ttgtcgatag
     8341 tgtttggctt gtattggatc gtttcggtcg aagcttttca tgactcatag tcaaatatcc
     8401 ttaaaactgc gaatgaggtg cttgacgatg tgaaagcgat ccacaacgag tttggcatga
     8461 ggaaagcact cttttatcag caaagcataa gaggcgttca tatctgttac gagtattttt
     8521 acgtttaaac gtgcacgtcg ttcaaattgc ataaaataag aaatgagatc tttcttctta
     8581 cgtgaaggaa gaatatccag taatttccca gtatcgccat ccatacaaga aaaagaatag
     8641 ggaccgactt gatttttcaa tgaacgaaat tcatctacca atgaacagcg aggcaaccaa
     8701 tttattttca cgtttgtcat ggggagcaga gaagcgagtg ttcgcgaaac ggttgaccaa
     8761 gaaacgccgc aatgtttaga aatcgtcgtc attgcttgtt tttcagtcaa ttcctccaat
     8821 attttataat gtacttgttt ggcgataaaa cagttttttt caacaatgta ggtattcgcc
     8881 gtaaaatagg tgtgacagcc acgacagagg taccgttgct ttgccaaagc aagataagtc
     8941 cgttgattcg cacacttatt gagaaggacc agcgattttt tctttccgtt cttaacgatc
     9001 agcccatgtt gattcacctg tttacactga ggacagggct gaatcgtttt aatgagcgtg
     9061 gcagaaacaa cgatactgtt gattgaatgt atttttttct tctcgacatg agtgatttga
     9121 atgtttccat ctttgattcc tagtaatagt ttgataagat gaagatcagg catacagaac
     9181 gctcctttga ttgtgttgtg gtgattcaat tctaacggag cttctgtatg ttttgttttt
     9241 ttatgctcaa aaggactgat agaatgattt actatcagtc ctagaaatta tagcgccaca
     9301 tttctttcac ctttgacttt tcagacggca gtttttatgc tgatactgta gagagagcag
     9361 gacacagctt ttgcttgttt tgggtaaggg ggaaagaaga tgaccaaagc acttattttt
     9421 gattttgatg ggacgatgat tgacagtgaa tggatgtttt acgagagtat tcatcactat
     9481 ttactgaagc ctatttactg gagcagttcg gctataaaat tccttttgca gactatcaac
     9541 aaggtgttgg gacggcagca gaactgtcct tttatgaagg gataagtcaa gcggttggga
     9601 gaccagtgga tgctgaacat tttgagcagc ggatcagcag cactggaaaa tcggagtagt
     9661 ctcaattctg aactgcggga gctagactat ttttttgaag cacatccgat gattaaggaa
     9721 aaaattgatt cctttgtgac gatcgatgat cttcaggtcg gcaaaccaga tccagaaggt
     9781 tatttgttgt gcttgaaacg gctgagggtt gaacaggctg ttgtagttga ggatagtcca
     9841 gtcggtgccc aagcagcacc gcatatgaca gttgatgtac aagcgttgga tgcagatttt
     9901 ttcgccttta gcgggcataa aatgtgcggg ccgacaggaa tcggtgtatt atatggcaaa
     9961 cgtcaattgt tagaacaaat ggagccagtc gaattcggcg gtgaaatgat agattttgtc
    10021 aatctttacg atagttcatg gaaagagctg ccatggaaat ttgaagctgg tactccgaac
    10081 attgcaggag caatcgatta cttgaatgag attggtatgg agaatattca tcgttatgag
    10141 caagaactgg ttgcctacgt tttacctaaa ttacaagcga tcggcggtat caccatttac
    10201 ggaccgcaag atcctgaaca tcatactggc gtggttgctt ttaatttgga tggcatccat
    10261 ccccgtgatg tggcgactgc tctggatatg gaaggtgtcg ctgtacgttc aggacaccat
    10321 tgtgcacaac ggttcatcga cgatttctgt ttgactaatt ggtcgtttaa acagtcgatg
    10381 ataatgccgt ttgagcggct ccaatgtcgt ggaatctttg agtaagagtt tccaatatcg
    10441 cttgagccga cggtacttct tctgattttc agagtgcgag gctcgaaaac gattcatgat
    10501 ctggatacgc aattgattga agctgcgatt gatatgttgg acaatatgaa agcgatcagt
    10561 tacgatctcc gcatttggaa agaccgtttt aagcagctga caatagctgg cattctaggt
    10621 acttgacctt taaacgggct tttctaggaa agtgcaggaa gtattgagtt agtttgtaaa
    10681 gtctgcgatc ttcaaggatc gtaaatagct gatgggttgt cccatcaaca caaataaaac
    10741 tcatggaact agaaacagac ttcattgatt tgaactcatc catacacaac accgtcggaa
    10801 ggaagttcat acgtggctgg taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc
    10861 catccgagac gaagtacttc ttagcgatta gtttacgtga ttgattttcg tacagttcta
    10921 tgagaatcgc ctgcttcagt tcctttgtta gttgatgatg ttcatcaact aagtccgttt
    10981 tcgcagaaaa ggtctgccca caatcatagc atagaaaacg ggtacgtttt aattctaaaa
    11041 tagtcaactt ttctttgact ttcaacatct gagtacgtgt cgtataggaa ccgttgcgga
    11101 taatacgaat cgatccacag tgttcacaat gggaaggtga gtcatccaat ctccctttaa
    11161 taaactgcgc ttgaacgcct ttatattctt tttcaatcag ccattcttca tcaaaaaata
    11221 aatgtttatc tgttaatccg agtaattttc tagtatgatt atccatagag cgactccttt
    11281 ggtagggtga tgttttggtc gactaaattt taccaaaagt gagtctcttt ttgagttgaa
    11341 acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caacaacaca aaatattata
    11401 gaagcctttt ttctaaagga tttcaaggtg tctgtttgca tttattaaaa aaaagaaaaa
    11461 gtattggcaa cagaaggcgt caccaacact agatattata gagccataat atgctgtata
    11521 atatctagtg tataggctta agtggaggta tatatgaaaa tattaactgt tgaagatgat
    11581 aatatgataa gagagggaat aagtgaatat ctttcagaat ttggatatac tgttattcaa
    11641 gctaaagatg gaagagaagc cttgtcaaaa tttaatagcg atataaattt ggttatctta
    11701 gacattcaga taccttttat aaatggttta gaagtgttga aagaaattag aaagaaaagc
    11761 aatttaccaa ttctaatctt gactgcattt agtgatgaag aatataaaat tgatgcattt
    11821 actaatttag tagacggata tgtagaaaag ccattttcat tgccggtctt aaaagctaga
    11881 atagattctt taattaagaa gaattatgga catttagaga agtttgaata taaggatcta
    11941 tcggttaatt ttaatagcta tacagctaaa ataaatggtg aagaaataga tgtaaatgca
    12001 aaagaactag aagtattaaa atgtttattg gataataatg gacaagtgtt aacaagaatg
    12061 caaattattg attatgtatg gaaagatagc gaagaaactc cttatgatcg agtaatagat
    12121 gtatatataa aagaacttag aaaaaaatta caattagact gtataactac tataagaaat
    12181 gtaggatata aattggagag aaaatgaaaa aattaaagat atttccaaaa atgttc
//
LOCUS       DACDVT010000071        12233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDVT010000071 DACDVT010000000
VERSION     DACDVT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.072"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(123..680)
                     /gene="frr"
                     /locus_tag="IWL52_002078"
     CDS             complement(123..680)
                     /gene="frr"
                     /locus_tag="IWL52_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9778610.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(683..1405)
                     /locus_tag="IWL52_002079"
     CDS             complement(683..1405)
                     /locus_tag="IWL52_002079"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9778611.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1541..2422)
                     /locus_tag="IWL52_002080"
     CDS             complement(1541..2422)
                     /locus_tag="IWL52_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9778612.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2521..3303)
                     /gene="rpsB"
                     /locus_tag="IWL52_002081"
     CDS             complement(2521..3303)
                     /gene="rpsB"
                     /locus_tag="IWL52_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9778613.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3662..4141
                     /locus_tag="IWL52_002082"
     CDS             3662..4141
                     /locus_tag="IWL52_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9778614.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4358..5449
                     /gene="phnW"
                     /locus_tag="IWL52_002083"
     CDS             4358..5449
                     /gene="phnW"
                     /locus_tag="IWL52_002083"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9778615.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5442..6227
                     /locus_tag="IWL52_002084"
     CDS             5442..6227
                     /locus_tag="IWL52_002084"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9778616.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6573..8264)
                     /locus_tag="IWL52_002085"
     CDS             complement(6573..8264)
                     /locus_tag="IWL52_002085"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9778617.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8686..9633)
                     /gene="arcC"
                     /locus_tag="IWL52_002086"
     CDS             complement(8686..9633)
                     /gene="arcC"
                     /locus_tag="IWL52_002086"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9778618.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9748..10767)
                     /gene="argF"
                     /locus_tag="IWL52_002087"
     CDS             complement(9748..10767)
                     /gene="argF"
                     /locus_tag="IWL52_002087"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9778619.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10858..12087)
                     /gene="arcA"
                     /locus_tag="IWL52_002088"
     CDS             complement(10858..12087)
                     /gene="arcA"
                     /locus_tag="IWL52_002088"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9778620.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3357 a         2540 c         2199 g         4137 t
ORIGIN      
        1 gaaatcattg tttttttatt taaaaactga ataatttttt ctttttttaa tttttaaata
       61 aaagaaatat aaataaaaac agaggaagcg gaatgatccg cccctctgtt tttatgattt
      121 tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac ttctttaatg
      181 ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc ttcggtaata
      241 tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat gttacgaatc
      301 gcgatttttg cattttctgc ttcttttttc acatctttag ctaattcttt tctgcgttct
      361 tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg actgattccg
      421 atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc aaaaggtgtg
      481 atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa tggtgttggc
      541 gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg gcctgcgcgg
      601 atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc ttttgcttct
      661 gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata ttttcgccta
      721 agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt ggaatatcat
      781 tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg gcaataactt
      841 ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga tcagcagaat
      901 aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt tctgccgctc
      961 gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct gcaaagataa
     1021 cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc tctgcgattt
     1081 gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc aacgtatcct
     1141 gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct tgagcacgtt
     1201 ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccaccc acaacgattg
     1261 ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct ttgattactg
     1321 gtggtttgat tccaaatcct gcatccccag ctaaagcttc tccactcaat tttaagacta
     1381 cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt gccattttac
     1441 catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt aaaaaaaggg
     1501 agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc acttggctca
     1561 taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact tcgaaacgta
     1621 cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc atatcaggat
     1681 ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc aagcgtcctt
     1741 ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt tctgtaagaa
     1801 cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca taacgaggat
     1861 tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt gttccatcta
     1921 gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat gcagcattgt
     1981 cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata acttgagttg
     2041 cttcgatcaa gtcagattca attgtacctt tatctgtttt gattttcatt gcagcttcca
     2101 tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa tcttggaaca
     2161 tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct gcagtgttac
     2221 ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc ttcgcagcct
     2281 ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct tctacttcaa
     2341 ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt tcttttacca
     2401 ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt ttaaaaaaag
     2461 ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca tcattcaaaa
     2521 ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga ttgatgttgc
     2581 tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt ttccttcgat
     2641 gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat cgtcgtttga
     2701 agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag ctacgattgg
     2761 gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt caacgatgta
     2821 cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt caagacgttc
     2881 acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag ttccatcttc
     2941 ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat cccagtttgt
     3001 caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag ttgcttcttc
     3061 tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc cgccttcttc
     3121 agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt tttgcaagtc
     3181 aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg gattccagcg
     3241 acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag aaattactgc
     3301 catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta ccttgccggc
     3361 aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg atgcataaag
     3421 caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat acttttctgc
     3481 tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag tagatttctc
     3541 tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga cgaatattat
     3601 tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa ggagaaagaa
     3661 aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat tcccgatccg
     3721 tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca cgcaggcaac
     3781 gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa atggcgtttc
     3841 ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg aagaggatcg
     3901 gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact ttttgacgat
     3961 cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg agatcaaacc
     4021 tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct cggaaaatga
     4081 ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat cactttttta
     4141 atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata ttttccttat
     4201 taaataaacc tttacactct ttacaaaaat acatcatttt ttattcaatt ttttcaacac
     4261 cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca gcttcttgct
     4321 ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat taactcctgg
     4381 accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt gtacttggga
     4441 tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc ttgctcaagt
     4501 atcggaagga atatacacaa cggtcttaat gcaaggtagc ggctcctttg gggtagaatc
     4561 ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa acggcgcata
     4621 tggacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg tttatgaggt
     4681 atcggaacat caaatcctcc aagctcatgt gattacacag ctgattgaag agaatccctc
     4741 tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga ataatttgga
     4801 agagattgct gctgtcacac aagaaaaaga tcttttgttt atagtggacg cgatgtcaag
     4861 ctttggtggg ataccgattc gtgtagccga actagggatt gattttttga tttccagctc
     4921 aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata aagcggaact
     4981 agaaaagcga aaaaatgtga gtcgaagttt gtcacttgat ctatatgacc agtaccgcgt
     5041 gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg ttttggcttt
     5101 tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat attcacgtta
     5161 tctcgccaat aatcatgtac tacgacaaaa aatgacagag atggggttcc agcctttcat
     5221 tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact ggcgatttga
     5281 ttttaatcat ttttatcagt atttaaagca aaacggattt gtgatctatc ccgggaaaat
     5341 cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg aagatatttt
     5401 ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa actgtaattt
     5461 ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac gcttttagaa
     5521 atgcttttct agaaaaagga atccaactga ctgacaaaga aatccgcgaa ccgatgggaa
     5581 aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa aaacaatgga
     5641 tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat caacaatttg
     5701 aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac acattggaaa
     5761 cgatcgaacg gttgagaaac gctgggatca atattggttc cacgactgga tttactgctc
     5821 ctatgatgac aatcgtagca gaaaccgcaa aaaaagaagg gtattcacca gacttcatgg
     5881 caacttctga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc cataaaaaca
     5941 tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat acggtctcag
     6001 atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa ggtagttcgc
     6061 taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaaa gatcgtttaa
     6121 aacagtctgt tgctcttaga ttccaacaag ctggcgctga tatttgtgta aacaatttaa
     6181 aagagttaac agaatacatt ttgtacgctg acaagaatcc agaatgactt ttatacaaaa
     6241 tcagaggtcg tgaaaaagct gtactgcatc aagtaataag aacagccaaa caaaaatagc
     6301 tcctcatatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
     6361 tattcgaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt atttacaaac
     6421 aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg actctcggta
     6481 tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct ctaatactcg
     6541 gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc atgaagtcct
     6601 agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc taagcgtgct
     6661 tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt gaatgcttgt
     6721 gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc agcagacaag
     6781 accgtttcag ggtatttttg aactaattta accacttccc agctggtatc gtcattcaat
     6841 gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct gactgcacga
     6901 gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac ttcttccagg
     6961 ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac tgccccaacc
     7021 ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc gctgatttgt
     7081 tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat ctttccttta
     7141 cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg gcgcatatcg
     7201 tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa atgatagctt
     7261 tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt acggtacaat
     7321 gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt aatcaacgct
     7381 ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc gttcagcaaa
     7441 tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa agcttcaccg
     7501 ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc tttcatcgat
     7561 tcatcacgga accattgcca taattgaata gcctcttcat cgccttcttc cagacgtttg
     7621 aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc atggaattgg
     7681 acatataaac gaagcaattc attgattggt tctgctttaa cagcttcttc tgtaccccat
     7741 tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa atggttgatg
     7801 cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc gataactgtc
     7861 gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat gtcgattggt
     7921 actgttcctt gattcccgat attgctatcc ccataatgtt ctttttcttt cactactgtt
     7981 tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta aggtccgaca
     8041 acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc agcgatttgc
     8101 tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac atctccgtgt
     8161 tcagctgatt ttggattttc tagtaattgt tcgacttgtt ccagtgttaa atcatctttg
     8221 acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat tcctcctaaa
     8281 gtgctccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta gtatgccatc
     8341 actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga ccagctccat
     8401 ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc ttcgttgtat
     8461 cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata caagtactct
     8521 cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa aaaaaaacga
     8581 aaggctagtc gtttctgata aaaaaacaat ccagatggcc taaaggagaa gaaataccac
     8641 ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat cagctacgat
     8701 aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag tgatgattgc
     8761 cttagcgttt ggttttgctt caacaaatgc aatagctgcc tctactttcg gaagcatgct
     8821 tcctggagca aattgctttt catcaatgta ttgtttcatt tctgaaacag taacagtttc
     8881 taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc ctgtcaatac
     8941 gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat ctttatcgat
     9001 tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc caccgccacc
     9061 aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg cttctttaat
     9121 cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat cttctttgaa
     9181 tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt agaatggacc
     9241 gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa cttgagtgac
     9301 taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca tggcgttttg
     9361 taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag gcattgccgg
     9421 tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt gcggaccatt
     9481 tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat actttgctgt
     9541 ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga tggcattgcc
     9601 ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt cagttctacc
     9661 gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat gctgactcgt
     9721 tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt tacctaatgt
     9781 tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa attgacgagc
     9841 atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa agcgttcttt
     9901 catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt gcaagaagat
     9961 caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt aaggtttcaa
    10021 caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata catcagaata
    10081 aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca attgtgcacc
    10141 ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt gtaattcttt
    10201 tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata aagagttagc
    10261 catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg ttccaaagtt
    10321 ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat cagtcaagcc
    10381 attccaaact ggaacaccag agaattctgc tagttcctct accatttttt gactgaatcc
    10441 acggaattcg attccgtcaa acatacgtcc tagtacttta gctgtatctt ctgtggattc
    10501 ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac ctagatcgat
    10561 cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata aaagcgcaat
    10621 attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt tcaagtgttc
    10681 tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg ctaataagct
    10741 tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg tttttattta
    10801 agattaaaga ggaaatcccg acggcagctg acagtaagac tgccgtcagg aaacaaatta
    10861 cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac gacctcgtga
    10921 caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca attcgttcga
    10981 tacataattt cggttataag tgactacaac acctggagcg attgccaatg tgtttgatcc
    11041 atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg ggatcagtac
    11101 aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg aatgagtaat
    11161 cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt ctttactttg
    11221 aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg tatccaaatg
    11281 catcattgca cggttgttcg gaattttgat tgccaacact ttttcaaatc ctgaattttt
    11341 tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt gagaaatccc
    11401 aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat gatctggatt
    11461 ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat gtttcaaaat
    11521 atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat tgactgtcat
    11581 cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca ttgggtccat
    11641 gtaaaatgga tagtcatcgt ctgctgacag atcatataga ctgctagaac gtacttcgat
    11701 atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt ccattgatag
    11761 aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt catccaacat
    11821 tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg ctaatttttc
    11881 caagtaaaga gtctcgacgc cttcattttg caatgtcttc gcaaagttgt catgttcttc
    11941 ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa tatctggtgt
    12001 caaattttcc acttcttgtc caggccgttt cagtaaaact gtctttaatt tccctatttc
    12061 agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg attacatgta
    12121 taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa tgagacattc
    12181 ctcacattaa tattcgataa ttaaataatt aaacgttttt ctatttttaa gcg
//
LOCUS       DACDVT010000072        12119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDVT010000072 DACDVT010000000
VERSION     DACDVT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.073"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..997
                     /locus_tag="IWL52_002089"
     CDS             <1..997
                     /locus_tag="IWL52_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778621.1"
                     /translation="KEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVEKINNFTQT
                     NQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQITSEALMEKH
                     EQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQDLAEQQMAI
                     EKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTP
                     SPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQEQARKE
                     YEQQLEELLNRTNQATTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            997..3606
                     /locus_tag="IWL52_002090"
     CDS             997..3606
                     /locus_tag="IWL52_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9778622.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYRQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            3603..5999
                     /locus_tag="IWL52_002091"
     CDS             3603..5999
                     /locus_tag="IWL52_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778623.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            6051..6383
                     /locus_tag="IWL52_002092"
     CDS             6051..6383
                     /locus_tag="IWL52_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778624.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            6384..6977
                     /locus_tag="IWL52_002093"
     CDS             6384..6977
                     /locus_tag="IWL52_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778625.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7140..9164
                     /locus_tag="IWL52_002094"
     CDS             7140..9164
                     /locus_tag="IWL52_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9778626.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            9164..10369
                     /locus_tag="IWL52_002095"
     CDS             9164..10369
                     /locus_tag="IWL52_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9778627.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            10385..11029
                     /locus_tag="IWL52_002096"
     CDS             10385..11029
                     /locus_tag="IWL52_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778628.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            11040..11846
                     /locus_tag="IWL52_002097"
     CDS             11040..11846
                     /locus_tag="IWL52_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778629.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            11869..12099
                     /locus_tag="IWL52_002098"
     CDS             11869..12099
                     /locus_tag="IWL52_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778630.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKIRSFEALYLAAKKYFTAERK"
BASE COUNT         4425 a         1928 c         2510 g         3256 t
ORIGIN      
        1 aaaagaggaa ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaattaa
       61 agagcgatac gaaaagaaac gcgacaatgc gtattccttg tgtgtagaaa agatcaataa
      121 ttttactcag acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg
      181 tataagagaa cgaaaagaag aattttatca aggtttgcgt acagataagc taaagattag
      241 caaagaatta aatgagcaac tgaataagct ctatcaaata acaagtgaag ctcttatgga
      301 aaaacacgag cagatccagc aagaattaca agtacaaatt ccaaaatgga aaaaagagta
      361 tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga
      421 gcgcgaacaa ttagaactaa aaaaacgtga acaggatctt gcggaacaac aaatggcaat
      481 agaaaaacaa cggttaaacc aagaaaagga aaaagaagca gctgctcgta aggaacgtca
      541 agaacaaatg gatacattac gacaagctca gttaaattat ctagcacaac ctcaaccaac
      601 gccttcagta gctgctgttc aaaccccaat gaacaaccaa acaaatctaa ctccttcacc
      661 acagcgttcc tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg
      721 tggagggaca gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtagc
      781 cacaacggcg tcaatcaagg aagaagccca agaacaagca agaaaagagt atgaacaaca
      841 attggaagaa ttattgaatc gtaccaatca ggcaacaaca gagtcaagta tggaaaatag
      901 caaagagagt gatcctagta aggctaaaga aggatcaagc gattcaactg tacaatcaaa
      961 cacttccgaa agttcagaat caaatgaggt gaaataatgc gtgttagaga aacaacgaga
     1021 cgaaaagaca aacgattcaa tttagaaaag aataggtatc tttctcattt ctatcgctat
     1081 actccttcag gagaagagct accagaaaaa catgacaaac gtatttctca aaagattttt
     1141 tatagtggct tagcttatct tcctttaaac tatgttgtta caagaatacc agaagcattt
     1201 catcgatttg agattattcc aggaagttta caaggagtga atatttttca gttacaagaa
     1261 atgccgcagt tattccattt tgattcaact ggtttttgga atcctttgac cccaacaaca
     1321 agtttaggca cacgtttagt tgtttctacg atttgtgttg ttccagctat acttatggga
     1381 ttgaaaatgg acatgtggtt tcgtcctcta gcagatgggc aaaaaggaga taaccgatta
     1441 aaaacgttaa aggaaatcca acaaatttat ccgcaaatac cagaatcaaa gggaggtttt
     1501 cctggttatg gcggaatacc aattactcat tataaaaagt actggtatat tcagacggat
     1561 acagtaaata cttgtattgt tggtacttcc cgttcaggga aaggacagat agaagtttta
     1621 gcaaccattg acaatttgtc tcgtgcagaa agacaaagtt caatggttgt taatgatcca
     1681 aaaacagaat tatatgttgc tagtaaagat gaactagaag ctagagggta tgatgtgtat
     1741 gcatttaata tcaccgatcc actgcaatca atgagtgata atccattggc actcatcgtg
     1801 aaatactgga agcgaggaga catagatact gcaacgcagt taaccaatac ttttacgaat
     1861 acgatttatt atgatgccaa tgcttcagac aacaaatact ttaacgaaaa tgcgcagaaa
     1921 gctgtaaatg cgcttatttt tgcgttgtta gaacaagccg atcaaacagg ggattactcc
     1981 aaattaacac ccaataatct tgttgagctt ctttctgaaa ttggcggttt taattatcaa
     2041 gatcctgaga atgattacag acaactcaat gccttagatg aattcatgaa tcagttacca
     2101 cctggaaaca tagcaaaaaa acaatatggg gcaacaaaaa ttggttctga taaagcaaaa
     2161 gggaatattt tatcgacggc aatcacagga ttgaatccat ttaccctaac gaaaattgcg
     2221 aaaatgactt ctcaatcaac cattgattat aagtccattg gttttccgaa gtatttggat
     2281 ctcaaactac acgagagtct gttaaatcag cgaattgttg tagagtttta tcatcaaggg
     2341 aaaaaatttc atgaggaacg tgtcaaagta ggttatagag gtttttgtga aatcaacttt
     2401 gattgtcatt tagagcaggg agatcatgtt tgcttaaaat acgatcatca aggaaaagaa
     2461 tatgttgctt ggtatgagtt ttctcaaaga gtattgaaag acgaagaggg aaatatcgtt
     2521 tatcgaaaaa aacgaggaga aacccattta ccagaattag aaaaagaagc tgttctaaca
     2581 ttagatgagg ataaaagtaa cttattagta gagtctattc ggatgcacta taccgataaa
     2641 ccaatggcta tttttatgct cacacctgat tatgatccat ctaaccatat cattgttagt
     2701 atttttctta gtcagttgta taaagaatta tctacgcaat gtgttaagac taaaggagat
     2761 aaatgtcacc gtaggattca ttttatttta gatgaatttg ggaatatgcc accaatggat
     2821 aatatggaag gaatcatgac agtcacagct ggtagaaaca tgttgtggga tctgtttatt
     2881 caatcctatc agcaacttta tgcgaagtat ggggaacaaa acgcagcgat tatcaaagaa
     2941 aattgtcaga cacatattta tattatgtcg acagacgata ataccatcga agaattttcc
     3001 aaaaaagtgg ggaataagac ggttgaacaa gagaacagta ccaccaattc attaggtatg
     3061 aatactcata tcaatcgcaa tgttgattca gatcgtatct taactcctga aagaatttct
     3121 acttttttag aaggtgaaac gattgtactc gctcctttaa aacgacaaga tttgaaaggt
     3181 cataaaatac ggccgtatcc tatttttaat catggggaaa cgaatcttcc ttatgcttat
     3241 caatatattg aagatttcaa tccatcgtgg gatatcaatg atatagacat acattgtaca
     3301 catgcggact tggatcttaa ggctttacaa attgattacg attattttct acctgatatc
     3361 tatgcacgtc gactttatca caatatgcac gatcaaatag aagaggaagc taaagaaaac
     3421 atttttatta aaatcgttca atctttagac aactcaacgg cacaaaagtt acttattcaa
     3481 ttttatgaag agaagcaaat agatcgtttg caagaatacc ttgaaaaatt gcatcaaaat
     3541 gaagaaatta cagaggttat aaggaaacga cttttgctag aagctgaaaa ggcggtgaaa
     3601 gaatgatcat tggaggaata ttttccggca ttgccaactg gtggtcaggc ttatttgggg
     3661 atggggatgc agatcccaaa accgtaacga aagtcttaca agattatggt aattatctgc
     3721 agtatcaaga tgtattcagt tggatttgta atactttttt atggggtttg attaaagggt
     3781 tatataatct caatacttta ctagaaaaaa caatttatca aagctttgat ttaaaagact
     3841 tattgaacgc tgctggtgtt aatgaactgt ttcaaagttt gattagtaag gtattggctc
     3901 ttttttgtgt cgtcacatta atttatttag gcttaaaatt ttttatgagt aaacatccac
     3961 caaaaatcaa aaatatcttg gttaatctat ttatggccat gattttgatt tttggtggta
     4021 gttctttaat cgatgaaggg ctaaatatta gtaaaaactt ttacggagac gtgactgcag
     4081 caaataaagc agataaaact ggatcacctg cttttcaaat tattcaaaat aatgtgatcg
     4141 atgtgagtac gctactagaa actgatccga ataaagtaga atccctacca acagataaaa
     4201 gaaatgctct cacggcgaaa aactttaaga cagcaaatat taattcagtg attattccta
     4261 aggaagccgc taagatggcg gatgaagata agagcggtct atcagatata caaaaggatc
     4321 ggctaagagc gttacaatac cgactggaac tggatgataa tggaaaagaa atccctgtag
     4381 agattagtga tgaggggtta gcaaaatatg tgtatacttc aggctatcgc cgatacatta
     4441 gtagtccagg gattatttta gctggtgaat cttctttgtc ggttgcctat ttatttatct
     4501 tgtttacgat tgtaacttgc attattgagc tggtatttaa aaaattctat ttagtaattg
     4561 ccgcgtcaac ggactttgag acaggacaac ggatgaaaag tgctatagaa gatatttctc
     4621 aatccttctt gttattggcg tttacgattt tagaattacg aatctataca ttaatgctgt
     4681 caggtatcgg tgatttgcac gcagcaggca agatcaacgg atttttgtat gttgtcgctt
     4741 taattgtact aaccattgcg ctattcaaag gctctcaatc tgttacgaag attttcggtg
     4801 ttgatacaag tctgaaaaac ggcagcaatt ctcttcttag catgtttgcc ttaggaaata
     4861 ttgcgaaaaa tgtgggtagt ggggctaaaa accttgcaag tgctggtaag agtggtttaa
     4921 gcaatttaaa tgatattcgt aaaaatggat ttggcaatcg aaaagatgtt attcctgata
     4981 acggggaaga acaaccagac gataaagcta caattgtacc taatagtaag aaaacagttt
     5041 ccgaaatgtt cgggaaagcg aaaaatggtg tttctaaagc cgcagaaggt cttggttacg
     5101 tcaatgaacg tgggttaaaa ggaacagctg aagatcttac agaaaaagcc aaagacacct
     5161 tagaagagaa attagacggc acgagagcta aagaattggc tgaagctgtg aagaatccat
     5221 ccgaagcact agaaaaacaa aaagaaaaag cttcagaagt caaagaagga ctaaaagatg
     5281 cgtatggtga aggacaagtc aatgcagcag tgaaggcaaa tacaacaagt gaaaaagaaa
     5341 atcaagaagg tgcagcgatt ccttcagcca aagatcacca agatgagtta cattctaacg
     5401 aaaattcgat cggagaaaaa gattcagaag aaggacaaca tggacagtta ccaaaaaccg
     5461 atccagaaca atatcgttat aaatctaaca ttaattctga aaatgaaaag cgcatttcag
     5521 atttaccaaa acgaaatcaa gaagtacaaa cggatgagga ttccatcgat ttgccaaatg
     5581 ataatggaat cgaagttgga actgaaaaat caatgaaaat tggtttgcct aagacacaaa
     5641 atttaccaat gaaagatagt cgtttgaatc aattcaagga gaatgatcaa agctttgttc
     5701 accaatcaac tgcaggtact gttcaaatga aaccaagtcg tttgcctgaa aatggaaatc
     5761 tgacaactgt ttcttacaat ttatctgaaa gtggtgagac agggaatgtg tccattcaaa
     5821 atgttgtgac aagagaagct gcaccagaac ctattcaaaa agttcacaca ggttacaaac
     5881 cagcaaatgt ggcatttact gcaccaacaa aaataaatgt aacaagagaa actaaaaaca
     5941 ggaacaatga attgaaagaa caactattga gggatatcaa acaggatatc aaaccataaa
     6001 aggcctacga gttaggcctt ttttctatgt atgaaaagga gaaatcattt atggataaat
     6061 atattaacat gaaaaatcct ccaagattta gtggggaatg gaagtttttc gcctttttct
     6121 ttttagatct attaacgctt ggtggttgct ttttttgggc gaaaatagtc aataatcttg
     6181 tccacttacc actctttttt gcactcttga attatggctt atggattgcg atagctctag
     6241 tgattgtttg gcgaccttat aataacccag gtcaacgaca aatttttgcg attatcgaat
     6301 ctattttctt tgcggataag aaccactatc atgcgttaga tcctaaccga aattatcatg
     6361 gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca aaattcagtt gataagaaat
     6421 ccattttgca gtttggaaaa ccagaagaac gatctatctt ggatttattt ccttataaat
     6481 cctgtacaga agagggagtc ttagttacta aagaagatca ttttcaacgt tattatcgtg
     6541 tagtttctac agacgtggaa ggattaaacg aacaagagaa actagaacga atgaatcagt
     6601 tgaccaccgt catgcgtacg tatgtgcaaa caattaagtt agttagtttg accacagaga
     6661 cggatctaac ggaacaaatc gtacaaaagc ggcaattatt agataagaat cgtatggaac
     6721 aatccttagg aagaaatcta cagcgattaa gaaaatatga aaagaaatta gtagaggaaa
     6781 tccaggaatt gaaacgagca gaacaagaac gtcctgactt aagcttcttt ttcgtcattg
     6841 aaggaaagac aatcaaggat ttacaaacca gaaatcgtca gttaatgcga tctagtggca
     6901 ttttagggtt aaaaccactc tacaaaaaag aattgatcaa gattctttat cgaatgaaca
     6961 atatgaatga tgagtaggaa tggagaagag agaatgaaat atccgttgat atggttagaa
     7021 aatgatgttc cgaaggaaga gatcactaga aaattttctc acactttaag cctgggtaag
     7081 ttgggaaaag ggcaatcttt taaccaagta aagaatcaaa caagaagagg tgactgtaca
     7141 tgttagaagc gattaaaaca gctttttcac cacaaaatga tcaaaaagta cttgatccat
     7201 taaagcaact aggaaatcgg gaaatagaaa ttttaaaatc caaagggtat gatttagatt
     7261 ttttaaaaca agtgatgcca caaggtggat taaatttcga tgaagagtat gtgacctatg
     7321 gaaatggtta ttcacgatgt gtgacagtct atcactatac aaaaaatcct actctttttt
     7381 ggttggccaa tctcatgaac aatcctcata cagtggccac actagatgtt agcacagatg
     7441 aaaagcaaaa agtgattgat caaatcaacc gcgcattaga tgaattaaat gatcgatcag
     7501 ataatgaacg aaaagcgact gaccgaaata aaagtgcgga agaatacctg gaactagcgg
     7561 aatatgcccg aaaattgact tctaatgggg aaatttctaa acaagtcaaa gtgagagtac
     7621 atgtttacgg aaatacgata caagaagtgg atgaacgggc gcaggaactg atcgaaaatc
     7681 taaaagggat ggattatcgt gcggtgactt atctgcataa aaccaaacga gattggcaat
     7741 cgatgtttac catgttcgat gaacaagctg aatggttcgg aaatatcaaa gggcaatcac
     7801 taccatcgtt aaacattggc ggaggtttcc cgttccacca ccaaacgcta aaagatccag
     7861 gaggtacaca atatggggaa accatgacag gcggcccatt tgtctggaat gcgacgtata
     7921 aaaatagtgt acggttgtct actaatacct tgcttctagg aatgatggga gctggtaaat
     7981 cgaccgttct taaaatgatc gcggaagccc atttagctgc aggagacttt atttggggct
     8041 ttgaaaaggg aaaagacttt attcctttct taaaggaata taacggtatt atggtgcgtt
     8101 tagacggatc tgacgggatg atcaatcctt tagagatatt tgccacacgg acgtatgatg
     8161 aagcatccag gttatatgac gatggatcag tcaaagattt gaagatcaat gaagcagcgt
     8221 cctatcaaac gcacttagac aaagtagtct atcaggtaca gttagtgagt cctcagctaa
     8281 aaggaacgat gaaagcagaa tttaagacct acttaaatcg tttttatgaa gagtatggaa
     8341 cagtaccacg tggctttact tcctcaaatt cgcgttcaaa tactgatacg caagtcacag
     8401 gtaaagatcc agaagcttat ccgacgttca aagaattttt agacttttta ggacaactgg
     8461 aattaccagg tgcttcccaa gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag
     8521 aatccttatg tgagacgtat ggcatgattt ttgatggtca cagtacgatt cgacaccttg
     8581 accaacaaca gttggtcttt tttgacattg actctattac aggtttagat cgaggggtgc
     8641 aacaatgtca aatgtacatg gctatgacgt tgatttggaa tcaggcattg atccagggaa
     8701 gaagacaacg acgtttaatt aaagaaaaac aaatggaaga acaagacttc aaacgcttct
     8761 tggcgatgat tgatgagtgc cataatgcga ttaatccaga ttttattgaa acggtaaatt
     8821 atgtcacaaa ctttcaacga gaaatgcgta aagtagaagc aatgactgtt ttagctacac
     8881 aatccccaca agaaatggtg ccagaaaata tgtcttctga taacttctcg aaactgaaga
     8941 aagtctttga attttcttct acgaagatct ttatgcgtat ggatgaatct attttacgtc
     9001 atatagaagg actagtgggt aaatcaatga cgaactcaga attagaatcc attcctcaac
     9061 aaaatcaagg agacgctgtg atcaactttg gatcaaaaga aaccattcgc gttcatttta
     9121 gtccgaacaa acgtcaatta aaattgtttg atggaggaaa ataatggaaa atcaagatga
     9181 aagtttaatc aagcagtatg ttaagcgtcg cgcaaagcgg cgcttatttt tgtggttgtt
     9241 tggtacttct gcaggtttga ttactatttt gatcactgtg tttgttaccc tatttctaat
     9301 acttgcggcg ggttcgattg ataattcgga ttcagattca agcagtggtg gcgaagcttt
     9361 tacaggagaa tattcggaag gcttaccgat ttataaagaa atcaaaggaa gaggcccttt
     9421 ttctgatgag attgcgcaat atgccgtagg tgcagcagtg aaatataaat tattaccaag
     9481 tgtgattctt tcgcaatacg gttatgaatc agcgtttggt acgtcggcgt ccgcaagaaa
     9541 tgatctcaac tattttggga ttacttggtt tgatggttgt ctgtttccta aaggaactgc
     9601 acgaggaatt ggtggcattg aaggtggctg gtacatgaag tttcccaact caaaagctgc
     9661 attctcctat tacggcttta tggtagccac acaatcaaat tttaatgcat gtgtaggaaa
     9721 taaaagccct ggcgcaagct tattgatctt aggacgaggt ggttatgccg ctgcaggaat
     9781 tactgaagat agtccgtatt atacaggttg tatgtcgatt attacctcaa ataaattaac
     9841 agaatatgat gaatttgcga tcaaacattg gggagaaggc ggaaatgaca acggtacgat
     9901 tactggtgaa tggacgaatc ctttccctgg cagcagttta gataaaagtt ccttcagcgg
     9961 tggtcaatta tttggaacga atccaggcgg tgaattccgt ccgaatggtt tccatgatgg
    10021 attagacttt ggttcagttg atcatccagg aagtgagatt catgcggttc atggaggaaa
    10081 agtcgtgtac gttggaaatc ctggtatttc aggtttgggc gcgtgtgtga tcgtgattaa
    10141 ttacgacggc ttgaatatgg tatatcaaga atttgcaaat agtacaggaa attcccgtgt
    10201 gaaagtggga gatcaagtga aagttggtca agtaatcggg atacgagata cagcacactt
    10261 gcatttaggc ttcactcgaa tggattggcg tcaagcacaa ggacacgcat tcacagatga
    10321 tggtacatgg atcgatccat taccattttt gaatagcagc aaaaaataga tcaggagaaa
    10381 aaatatggaa aataaaacgt taaaaatcgt cttaacagct gcactcagca ttagcttaat
    10441 tggcaatatc gcgctctttt ggcagctgaa agaggaacaa gcgacaaata cctataaaat
    10501 tgaagaagtg gctaaaaacg cgtctgagaa gctcaaaaaa aatttctctg aggcacaggc
    10561 gaagatcgac aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg atcagctgaa
    10621 gcaacaagac ggtataaaag caaaagaaga agtcacagat gcccaaagaa agacggcaga
    10681 agagttcggt caattggcca ttgataaatc actaagcaga aatgaattaa cggaaaaact
    10741 aaaagatgtc gcaacccaag aagtgatcga caaattaagt cctgcagatg atgatcacca
    10801 acatgatgat tatggaagtt actcctttac attaggtgac gtgcaaactt acgctgaaac
    10861 aagcagtgta aaagaggaac aaaattttgt cgtatttgtt gattatacaa tcggcaatcc
    10921 tcaattcaaa gatgtcaaac cgcaaaaaat aaaaggtgga ttaactgtca ctgaaaaaca
    10981 agtagatggt caatggaaag tcacagactt tagttatttc actcgttagg aggaagaaaa
    11041 tgagtaagaa aaaaggagca agacaacgtt ttaaaaaacg gaaaagttat cttagatggc
    11101 tgggaatctt tacgattctc ggctttttga ttcttgctag tccgatttat acgcataaga
    11161 aggtaaacat accagatgag gtaagcacga acgctttttt aaacacgaaa gaactgacaa
    11221 tggtatcaaa agaaaaagga gcgaatcagc aattagtttt tgaatttgcg attgacgatg
    11281 aggatcaagg cttattaaaa gaactggcta atcttgatta tcgcgtagaa gtaaagacag
    11341 ctaaaggtga ttatcaagca ataaaagcca aagtgatcaa ggtgtctgat gattattttg
    11401 tagtgaaatt agcacatgta ccagaagaat atatcgccat gcgtttaacc attgttcctg
    11461 aaaaaattga tccaaaagtg gatatgcaag aaccacaaga tctgatttat tacgttcacg
    11521 aagataaggt taaggatcaa gtgaaagatg ataattatga acaacatgcg atcaattaca
    11581 aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa acagattgaa tccttgcaag
    11641 caaccattga tttgaatgaa gaattagtga ataaactaaa agaccaattg ccttatcaag
    11701 tcgcagatga tcaggaaaat actgaaaata agattaatga ttaccagcaa gaaattgaaa
    11761 catcgaaaac gcaaatgaaa gatcaagaag agatcattca aaaaattcaa gaaaaaattg
    11821 atcgtatgac aaaagaaaat atttagtaga acaggtggag tgaaagaaat gaagggagcg
    11881 aacttggtcg gcaaagatat tttcgctgag cagaaagtat tgattaaagc ctatctcaaa
    11941 gttgtaaaag ctaaagaaat atgtcaagaa gtgaatttat ctaacacaac aatatctgag
    12001 ttgaagaatg gtagaaagag tttagatacc ttaaaaatca gatcatttga agctttgtat
    12061 ttagctgcta aaaaatattt tacggctgaa agaaaataaa aacaggagga ttttatgtg
//
LOCUS       DACDVT010000073        11800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDVT010000073 DACDVT010000000
VERSION     DACDVT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.074"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            514..1077
                     /locus_tag="IWL52_002099"
     CDS             514..1077
                     /locus_tag="IWL52_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778631.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1138..1503)
                     /locus_tag="IWL52_002100"
     CDS             complement(1138..1503)
                     /locus_tag="IWL52_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778632.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1579..1824)
                     /locus_tag="IWL52_002101"
     CDS             complement(1579..1824)
                     /locus_tag="IWL52_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778633.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1894..2148)
                     /locus_tag="IWL52_002102"
     CDS             complement(1894..2148)
                     /locus_tag="IWL52_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778634.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2312..2635)
                     /locus_tag="IWL52_002103"
     CDS             complement(2312..2635)
                     /locus_tag="IWL52_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778635.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2822..2895
                     /locus_tag="IWL52_002104"
     tRNA            2822..2895
                     /locus_tag="IWL52_002104"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2856..2858,aa:Ile,seq:cat)
     gene            complement(3280..3777)
                     /locus_tag="IWL52_002105"
     CDS             complement(3280..3777)
                     /locus_tag="IWL52_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9778636.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3817..4626)
                     /locus_tag="IWL52_002106"
     CDS             complement(3817..4626)
                     /locus_tag="IWL52_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9778637.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4960..5817
                     /locus_tag="IWL52_002107"
     CDS             4960..5817
                     /locus_tag="IWL52_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9778638.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5810..6712
                     /locus_tag="IWL52_002108"
     CDS             5810..6712
                     /locus_tag="IWL52_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9778639.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6745..7095
                     /locus_tag="IWL52_002109"
     CDS             6745..7095
                     /locus_tag="IWL52_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9778640.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7129..8067
                     /locus_tag="IWL52_002110"
     CDS             7129..8067
                     /locus_tag="IWL52_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9778641.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8145..9038
                     /locus_tag="IWL52_002111"
     CDS             8145..9038
                     /locus_tag="IWL52_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9778642.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9076..9639
                     /gene="amaP"
                     /locus_tag="IWL52_002112"
     CDS             9076..9639
                     /gene="amaP"
                     /locus_tag="IWL52_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9778643.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9653..9841
                     /locus_tag="IWL52_002113"
     CDS             9653..9841
                     /locus_tag="IWL52_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9778644.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9854..10756
                     /locus_tag="IWL52_002114"
     CDS             9854..10756
                     /locus_tag="IWL52_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9778645.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10788..11030
                     /locus_tag="IWL52_002115"
     CDS             10788..11030
                     /locus_tag="IWL52_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9778646.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11286..11657)
                     /locus_tag="IWL52_002116"
     CDS             complement(11286..11657)
                     /locus_tag="IWL52_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778647.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4190 a         1985 c         2233 g         3392 t
ORIGIN      
        1 aaaaattcat tggtcgttga atgggctgaa tagtagtgta taaaacaatt ttgtggagcg
       61 tgaagctttt atggtagagg aaaatacgaa taaagtgatg ttgataaaaa gattcctatc
      121 ttgaatatga atcgagatgt cttgaaaatg aatgagtttg accaatcatt atcctatcga
      181 aatttacagt ttcgttcgat tggtctgtca attaatgaaa aagtaaaaaa gaatatgttc
      241 taggaagagg gcggcacatc taaattttta agtaacggac agttcaaata tcaagcattg
      301 aaagaggcta tcctactgtt tgaaggaata cgaatatata aaaacgacaa agaggaaaaa
      361 gtagttcatt aaatcccttc aagtgatgaa agaatgagat ctaataaaac ctgatctttc
      421 atctgttttt ttaataacgt aataattttt ttattttttg tgctttcgcc gttattgtag
      481 agtgatttag cattcttatt tttaaaaata actgtgttat atattaaaaa aggcataata
      541 gttataagct tttttaaaga atatttagaa attattaatt ttcgtctgaa aacaaaaaaa
      601 gaaagcgtta ttattttagt ctattttcag ggggggaacc ttatgataaa aaaactattt
      661 ttactatcaa tattaacgct agcaatggca ggttgttctg actctggctt agcaactgaa
      721 ctgatcacaa aatcaactga agccacgaca gaggagttaa aatgtggaaa cgatcacagt
      781 gactacagag gcttaaatcg acgatgcatg ggaatattag gcagaagcaa ttgtaagcat
      841 cgatgtagta atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa
      901 agaagcaatc tgtgctgtgc atcgtataca gacgtcgaag atgcggtact tttaaaccac
      961 gtcaggagat gttcatttat gtctagggaa gttggcgatg aaccaaaact agagtacgct
     1021 tttagttttg cacgtgagga tgaagataac cctaaaactt acgacatttt agcttgatct
     1081 aagatggtta aagcttttta atgaagtaag aggagataga aggcttttct agttatatta
     1141 ggtaagataa attccttttt gctccaaatt tgctaaacat tctgctagat aaacttcatc
     1201 gtgttccgga taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca
     1261 tgatttgcca cgatacatag gatttttcca ctctggtgct gggctgtaac cacgtttttc
     1321 catttcatcc atgacaagct gatgatattg gaataatcta taaggagaat agtgaaaaac
     1381 ataatccaca gtagcatgtt tctttcccca gccatttcct cttaatgcac agcattcccg
     1441 atgttgtcct aaaagctgtt gacgaggaag ctttgaaatc aatttttcat gccataatcg
     1501 catagaaatg tctcctttat atatagattg ttaaccttag acgttaaatg aaaaaaccag
     1561 caccaaggct ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac caattaggta
     1621 atcaacggaa atatcgtaaa agtcagcgat atagattaat tcaatgatgc ttggaatttc
     1681 ttctccttct tcgtacttac gatatttgaa taaaggaata cttaacgcgc tagaaacagc
     1741 tacttgagat aaagatcggc cagttcttaa gaaattcaga cgacaacgta aagtatccag
     1801 taaaatagct tcttcgatac tcatttaatc acctttcatt atagtagaat agttataatg
     1861 actatagaaa gctacacatg caatcaatag tactcaatgt tttttattta aacgtatcaa
     1921 ataaagaatg acgatcaata aagacaaaac cagtcctgat aattcttcta taaaaacact
     1981 atcgggttca aagtagttgt aattaaaaac tttccacata aagtagatag ccggaagagt
     2041 caaaatagct aaataaaacc gttctttgtt ccaattcata agataaacaa tacttgcgac
     2101 cagtgcgacc gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt aacggactcc
     2161 tcaacgattc atataataaa agataacatg ttattgcata catcacaaat gaaatgctag
     2221 gaaaataaaa gaagaataga tcaatgaaaa atgaaaaagc agaacagttt tctgctgata
     2281 agcaggaact gttccgctga atgtgtctta tctatcgaga aaaaatcaat ggattgatat
     2341 ctaagtaatt caatacatat aaaaagagga cgatcgcatt cactaaaaag gaaagaatga
     2401 tgatcccata gcgaatggaa acatatttaa caaatacttt tgtttgaata cgggcatatc
     2461 tttttccttt atagctgagc atcatgacaa gaagcaaaag ggtaatcaca ccttgaaaaa
     2521 tcattccaat aaacagaatt cctgaggttt gataatttaa ttggagaaaa taagctgctg
     2581 ccgcaactaa atcgaataaa atcgcaataa atggcaaaat aatcactcct aacattgaga
     2641 ataaataaaa gaataacatt ttttctaaaa taagcaaata aaaattaaag taacaaaata
     2701 gttatgacac aagtaaaaga aataagaata acaatattat tttacttata ttttgaaact
     2761 tgtgagagtt tgtcattcgt gctatagttg aaaagtaaca aaaataaatt ttatttatga
     2821 tgtaccttta gctcagttgg ttagagcaga cggctcataa ccgtccggtc gtaggttcga
     2881 gtcctacaag gtacataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     2941 ccaagtaaaa acagcttctt atgtttttac ttggctgttc ttattactgc agatgcaagc
     3001 ttttgaatac cgtttaagga agaggttgtg aaatcagcgt tttgacctga gtattagctt
     3061 aacaaacgga aaaatagttc ctcatatttt gccgtttgtt aagctaatac cgagggtcag
     3121 ctgattgaac accatttaag aaagaggtcg tgagcctgcc gtttcactct gtgtcataaa
     3181 aaagccaatc tactttgata gaaaaagcac gtaggactaa aacagttctg cgtgcttttt
     3241 ttattgtgtg ttaaatatcg taaatgaaga tagatatttt tatgattcga cgatagagac
     3301 gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt tttctactgg agactaacat
     3361 tggatcaaca aaaaagtctg cttcaggaaa agcactctgc aggcctatca gattagagag
     3421 tacacacaag tttgtttcaa tcccgactac ttcgatctct ttttgttctt ctttgctttc
     3481 aaataaagtt tcctggattt cagctaaagt ttcaggtgga atcgtaaagt aatacttttt
     3541 aatcactcgt tcattttctt gtggagaaag aagagggatc aattgcaaat cttctcgttc
     3601 ttcttctttg tccttcagtt caactggtat atcccgtgtg aagagaatat attcattatt
     3661 ttggcgtgct ttagctaatc tttgagcaat acgttctgcc aattgatctg actctggaat
     3721 ataaaattcg ctgtttggat caagaatatg attttgcata tcaataataa ctaacatttt
     3781 ctttcctcct acattcatat cgtcatttta ttctttttaa tagaagtttt ttaaaatata
     3841 agcaacacca gcttcttcat tcgtcagtgt tacttggttt gcaatttctt ttacttgtcg
     3901 ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc aacatagcaa tgtcgttttc
     3961 ttgatcgcca aaagccatga tgtcactcga ttcgattcca aggtaatccg ccagaaaaac
     4021 aagtgaatga gctttagtga tacctgtagg cgtgatttct aagaaatgcg gatcagagaa
     4081 tactgattgg ataccatctg cttgcagatc tctttgtagc ttctgtagtt tttcttcact
     4141 cttatctgtt aatgctaatt taagatagct aagagaagga tcttccaaca tatttaaaat
     4201 ttgatcaaaa gaatggttct gtaaattgaa atgttcataa taagggtcat ctttgactag
     4261 atttgattca tcgaatgtca gctgatcatc ttgggtttct cggctagcaa tgatggatat
     4321 tttcgcttct tgagcataga agaaggcttt tctcagtgta tcgctatcta tttttgaacg
     4381 tctgagaact ttcgccattt ttccatcctt catttcagca atcagtgaac cattggaaga
     4441 aatcaaatag ccaccgattc ctaattcgga aaaaatatct ttagcagaaa tgctgttcct
     4501 tccggtagcg acggttacga ttccgccatt ttcatcaaat ttccgaagtg cttggatatt
     4561 ttcttctggt atctttccat ttcgatcaag caaggtaccg tctaaatcaa tcgctgctag
     4621 tttcatttta tcttcctact ttctttttac atatctattt tcctgttacc ttaagattaa
     4681 atgagaattt aaaaaaatga aactaacttg ctttgtcatt atgaattttt tcaaaaaaca
     4741 ttcttaagaa aaaggtggct atatatagta gtttttggtc aatacctttg aataaaaagc
     4801 catgaaaaac gaatagttag cgcttaccga tgcgttttgt gataaaaaaa ttttaaaata
     4861 ttcaatgaaa acaaagcata tattttgctt taaaaccaaa tgaggaatat tctagatgta
     4921 gaacaggtta gaaaaagatt agaaaaagga gtgattttga tggagaagag atacggcgta
     4981 gttcctgtgg taactgcttc agacgaaaat tatgctcctt acttgagcgt gatgatcgca
     5041 actgcattag aaaattgcaa taaaacaaga cgcataaaat tttatgtgat tgacgatggt
     5101 ctatcagaat atagtaaaga aggattggaa gaaacggtaa ataaatattc aagtaatgca
     5161 agtatccaat tcctgacagt ggaaaaagat atctacgaag acttcctagt cagtgaccat
     5221 atcacaacta ccgcttactt aagaatttct ttaccaaact tattagctaa agaggattat
     5281 aagaaagtcc tttacttaga ttcagatgtg ttagtactag acgatatcgt aaaactatat
     5341 gatgagccgt taaatggaaa aacaatcggc gctattatcg atccaggcca agtgaaagct
     5401 ttggaaagat taggaatcga ttccgatgac ttgtacttca attcaggagt gatggtgatt
     5461 gacattgatc aatggaataa aaaagaaatt acagaaaaaa ctatccatta cctcagtgaa
     5521 aatggcgatc gaatcatcta tcacgaccaa gatgctttga atgctgtact ttatgaagat
     5581 tgggaacaac ttcatccaaa atggaacatg cagacttcgc tgatttttga acgtcatcca
     5641 gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc ttcgatcgtt
     5701 cactttacag gacatgataa accttggaat acgttgaaag atcatccgta taccaattta
     5761 tacttaaaaa aattagcaca cagtactcta acgaaagtag gcgagatcaa tgaataaaaa
     5821 agaaatagca gtagtcgcaa gttgtaacac aaaattcgta ccacatttag ctgcattatt
     5881 tgtctcagta ttagataatt gcaacccgtc taaatttgtt cggttctatg tgattgacga
     5941 tgatatcgat tttgaaagta aacagctttt acgcttctca gtgaaaaacg cacgaatgaa
     6001 ctcagatgtg gaattcttga aaatcaataa agaattcttt acaaatgtgg taatcagcga
     6061 tcgtattcca gaaacagctt attatcgtat tgcgattccg gaattgttcc gcggcacaga
     6121 agttgaacgt atcttatata tggattgcga catgatcgct ttacaagata tctctaaatt
     6181 atggcgtctt gacttcggtg attcaatcgt agcagctgtt gaagacgctg ggttccatca
     6241 acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact caggtttgat
     6301 gctgatcaat gtgaaaaaat ggttagatga aaatattacg caaaaagtat tagatttcat
     6361 cgaacataat ccagaaaaat tacgattcca tgaccaagat gcattaaatg ctatcttaca
     6421 tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa ggctatatca tggcaaaagc
     6481 aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa gaaactcgca acaatcctta
     6541 cattatccat ttttcaggac atgtgaaacc gtggagcaag gattttgaag ggccaacgaa
     6601 gaaatattac gaaaaatatg cagggatgac ggctttccgt tgtgtagcta aattccctaa
     6661 atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat aatagaagac
     6721 aacaactgaa gagggggagt aggaatggaa attacaatgt ctaaaaatgc agtagagacg
     6781 ttaattgaaa aggttgggga aaatacaaaa atcgcactcg cgcttatcaa tgattccgat
     6841 ccatttctaa gggacaaagg tgcttttgca aaaggcagct tttttcaaat cattcctttt
     6901 gtttcggaat ttggagaata tgcgactaaa atcgagcatc cattattgga catctataca
     6961 tctaaattag agcaaaatta tttcggtaaa cgattgaaca tggacttcaa caaacagctt
     7021 gacagctttt cattagaaaa tgaaatcgca gtactcgatt ataatatcaa attaaagaac
     7081 tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt gagatactac
     7141 tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa ttggctcgta
     7201 cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga gcttcctgat
     7261 gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg cctttatgag
     7321 acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc agataatcgt
     7381 atcgaaaaaa aattaacgcc aatatctttt gtactttccg ataatctctt gattacttgt
     7441 atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa tcatttaaac
     7501 tcgtttgaaa agatcatcac gtatacatta ttagatattt acacccatta cgtgaaagaa
     7561 ctaagagaga tgaaaaagcg aattgacgct ttggataaag cagctcgtaa aacaacggaa
     7621 aatgaagaac tgtataaaca ggcagatctt gaaagagaca tcgtatatat cgatcatacc
     7681 ttaagagatc aacgggaaac aatggatcaa ttatggaatt ctgaaacttt catcaaacgt
     7741 ttagacgatg aacagctact ctatgatgtc catttgagac agcgacaaac agaaaaaatg
     7801 atcgagatct atcgagattt attagagagt attggtgatc tattcaacgg gatgatggac
     7861 aataatctaa atcatatcat gaaatatcta gattcagctg cattaatcat ttccgttccg
     7921 gcgttgttcg cagggatttg gggaatgaat acaggaggat taccgggtaa aagttctgct
     7981 ctaggcttta tactcgtagt ctttggctct ttgatagcag cagtagtttt aggttaccat
     8041 ttataccgga aagattatac aaattaaaga atctatttaa aacaggtatc aaacaaagaa
     8101 atttgttttg gcaatacaat ttaaaaacta ggaggaatac agcaatgaca gaaccaaaat
     8161 taatcgaccc gcgaaacttg taccatacgg agaaatttcc taaacaagat caggatacac
     8221 cagctttaca gcagaatatg attcctgttc ctgattgtgg agaagaatcc tatgaaggca
     8281 ataatcaact agaaaaccgc cgtgtactaa tcactggtgg agattcaggg atcggtcgtg
     8341 cagcagcgat tgcatttgct agagaagggg cagatatcgc tcttcaattc ttcccaggag
     8401 aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaaga tgcaggacgt aagattgtct
     8461 tactaccttt tgatcttcgt gacgaacaag cacctaaaga aatcatagat aaagccgtga
     8521 gtgaacttgg cgggctagat acgatggtac taaacgcagg acagcaaatt tcttgtcagt
     8581 ctattgctga gttaccactt gagcaagtca aagacacatt tatgataaac atcatcgcta
     8641 tgtttgcttt agtgaaagaa gctgttccgc atatcccagc aggaggagca atcgtaacaa
     8701 caacttctgt tcaagcattc aatccaagcg aacatttatt agattacgca gcaacaaaag
     8761 cttcaatcgc taactttacg atcggtttag caaaacagct tgcacctaaa ggaatccgtg
     8821 taaatggtgt agcacctggt ccaatctgga caccattaca actagacgaa ggacaacctt
     8881 ctgattcgat ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt cagccaggag
     8941 aattagcacc agtttatgtt ttcttagcat cgaataaagc aagttatgtt acagcccaaa
     9001 tttacggagt aactggtgga gaagcgatca acttataaga agttgatcgt ttcaccccta
     9061 tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg aacattactt
     9121 ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt gccttttcag
     9181 ctagagaagt ttcgattctt ttccatcact aattcatacg tgcaacaata tgtattttgg
     9241 cttgccgttg catttgctat tttgacaatt atcacgttac ttgttttcct gttttatcca
     9301 aaaacaatcg gaaactttct actgaaacat ggtgatgggg agttgacgtt ggataaaaaa
     9361 gcaattgaag gaatggttcg ttctcacttg catgaagatg aattcatcca ttcaccaaaa
     9421 gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg taaaagggga gttgaaacga
     9481 acttcagcac tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa tgaagtacaa
     9541 cacatattgg gaacaaaaga gccagtgaaa gtatcggtga aatacacagg ctttgaagaa
     9601 ccagctaaac gaagcaatga ccgcaaacga gtagaatagg gggggaccaa aaatgaaaga
     9661 actattttcg acatacaagc tccctatcat ctttggggta cttggattga tattagccgt
     9721 tttatggatc accgtgggct ttttcaagac cttattgatc ttgatcatgg cagcgatcgg
     9781 cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta gatcactatt ttaaatctta
     9841 ggaggaaaca tccatgaata acaataacaa aaataataac aataaaatga acaaccaaaa
     9901 accagtgtct gcacatccag acccaacgac accaaaccaa tcaaatggtg tgaacgatga
     9961 aaagaacaaa acagcaccag tatctgcaca tccagatcca gtagtcccta acgttcaaca
    10021 aggagtagaa gagaagaaac atgatgcaga aaagcatgca cctcatcatc caggaactcc
    10081 agaatctaaa gttccagaac aacatgatgc aatcgatcct tctaaacctg tggaagagca
    10141 aaaacatcct tctgaccatg cgaaagcaca agaaaaactt agttctgacc acacaaacgg
    10201 acaaggaaaa cttggttctg atcatacaaa taaccaagga aagcatgatt ctgaacagga
    10261 agattcaatg cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa tcatcggaat
    10321 cgcaattgaa caagtagacg gtttattatc tgcaaacggt ggtttcttct ctaatgtggc
    10381 aggaaaactt gtgaatacag ataatgtaac ggctgggatc gaaacagaag tcggtaaaaa
    10441 acaagtagca gttgatatgg atgtcattgt ggaatatggc aaagatatcg aaaaaatctt
    10501 tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat atgacacatt tagaagtgat
    10561 cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa aagatcaaga
    10621 aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg cttcgaatca
    10681 aacagataaa gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag aaaatacaga
    10741 accacgggtt cagtaataag gaagtaaaac attaggagga acgaaacatg ggatttattt
    10801 ggtcacttat cgtcggagga attatcggag caattgcagg agcaatcacg aataaaggtt
    10861 cttcgatggg gattattgca aatgtcgtag caggtttagt tggatcagca cttggtcaag
    10921 cattattagg ttcttgggga ccaagtattg caggaatggc aatcgtgcca tcattgattg
    10981 gtgccatcat tttagtacta atcgtatcat tctttgctcg aaaactgtaa aaagactttt
    11041 aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata agaacagcca aacaaaaata
    11101 gcttctcata tttttcattt ggttgggcat attatcgaag atgcaagctt ttgaacactg
    11161 tttatacgga ataagaggct gtgacaagac ttttgtcaca gcctcttatt tatgcgggca
    11221 aattcccaat tagattttta aaagtttttc cttaaatgtg tctttattga tacagagaag
    11281 cttctttaat aaaacaatgc ctccctaaat tttcttgaga taggaaaggt gaacatacaa
    11341 aataatacga ccactttgaa aacccagatg atccacaaat ggatcgtatg atactgatga
    11401 agtaaaaagc aatggatcag aaactcaaag agattgaaaa agacaaggaa gaaataatat
    11461 ctctttttta ctcttgtctg ttttagccaa taatagctcg taaagtaatc tagacaaaca
    11521 aatagaagta aacaaagttt cgtaagagca gcaatcgtat catgttgctc tgctagacga
    11581 ttgacgagca ggaaaaatgt tggtaatgga atatagatca cgagtaatga aagaaaaaag
    11641 taaacaataa atggcatact attcctccta attgtgtgag ttcgccctca ttttaaaata
    11701 acgatatttg tttgtgaagc tttttcgtgt cgtaacaaat gttatctaat gtatttttaa
    11761 ataaataatc attattatca aaataatgtt aaaaataaaa
//
LOCUS       DACDVT010000074        11717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDVT010000074 DACDVT010000000
VERSION     DACDVT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.075"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(179..586)
                     /locus_tag="IWL52_002117"
     CDS             complement(179..586)
                     /locus_tag="IWL52_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9778648.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(775..1602)
                     /locus_tag="IWL52_002118"
     CDS             complement(775..1602)
                     /locus_tag="IWL52_002118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP9778649.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1911..2129
                     /locus_tag="IWL52_002119"
     CDS             1911..2129
                     /locus_tag="IWL52_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9778650.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2150..2785
                     /locus_tag="IWL52_002120"
     CDS             2150..2785
                     /locus_tag="IWL52_002120"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9778651.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="IWL52_002121"
     CDS             complement(2978..3217)
                     /locus_tag="IWL52_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778652.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="IWL52_002122"
     CDS             complement(3241..4764)
                     /locus_tag="IWL52_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9778653.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="IWL52_002123"
     CDS             complement(4782..4904)
                     /locus_tag="IWL52_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9778654.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="IWL52_002124"
     CDS             complement(4934..5917)
                     /locus_tag="IWL52_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9778655.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWL52_002125"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWL52_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9778656.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="IWL52_002126"
     CDS             complement(6111..6488)
                     /locus_tag="IWL52_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778657.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWL52_002127"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWL52_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9778658.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWL52_002128"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWL52_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9778659.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9278)
                     /locus_tag="IWL52_002129"
                     /pseudo
     CDS             complement(7506..9278)
                     /locus_tag="IWL52_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(9232..10986)
                     /locus_tag="IWL52_002130"
     CDS             complement(9232..10986)
                     /locus_tag="IWL52_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778660.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="IWL52_002131"
     CDS             complement(11096..11665)
                     /locus_tag="IWL52_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9778661.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3697 a         2261 c         1692 g         4067 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagttcg agataaatct gataaattat gttatgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
    11701 aaaaatctgt tctatca
//
LOCUS       DACDVT010000075        11411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDVT010000075 DACDVT010000000
VERSION     DACDVT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.076"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..776)
                     /locus_tag="IWL52_002132"
     CDS             complement(<1..776)
                     /locus_tag="IWL52_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9778662.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLL"
     gene            922..1725
                     /locus_tag="IWL52_002133"
     CDS             922..1725
                     /locus_tag="IWL52_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9778663.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1903..2850
                     /locus_tag="IWL52_002134"
     CDS             1903..2850
                     /locus_tag="IWL52_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9778664.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2918..3211)
                     /locus_tag="IWL52_002135"
     CDS             complement(2918..3211)
                     /locus_tag="IWL52_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9778665.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3239..3904)
                     /locus_tag="IWL52_002136"
     CDS             complement(3239..3904)
                     /locus_tag="IWL52_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9778666.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4044..4676)
                     /locus_tag="IWL52_002137"
     CDS             complement(4044..4676)
                     /locus_tag="IWL52_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9778667.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4683..5750)
                     /locus_tag="IWL52_002138"
     CDS             complement(4683..5750)
                     /locus_tag="IWL52_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9778668.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5747..6478)
                     /locus_tag="IWL52_002139"
     CDS             complement(5747..6478)
                     /locus_tag="IWL52_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9778669.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6647..7126)
                     /gene="greA"
                     /locus_tag="IWL52_002140"
     CDS             complement(6647..7126)
                     /gene="greA"
                     /locus_tag="IWL52_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9778670.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7262..8437)
                     /gene="mltG"
                     /locus_tag="IWL52_002141"
     CDS             complement(7262..8437)
                     /gene="mltG"
                     /locus_tag="IWL52_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9778671.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8641..9810
                     /locus_tag="IWL52_002142"
     CDS             8641..9810
                     /locus_tag="IWL52_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9778672.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(10077..10991)
                     /locus_tag="IWL52_002143"
     CDS             complement(10077..10991)
                     /locus_tag="IWL52_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778673.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(11049..11411)
                     /locus_tag="IWL52_002144"
                     /pseudo
     CDS             complement(11049..11411)
                     /locus_tag="IWL52_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3598 a         2213 c         1751 g         3849 t
ORIGIN      
        1 cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt ttcgctatac
       61 tgccaaatat ggacatgatg aacatccttt accccatcga ttgcttcaat ttcttgttcc
      121 attttttcat agtcgatagc aggtgcactt tgtatcaaaa tagcggcagc ttttttgatc
      181 accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac tggatcgatc
      241 cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac cgaagacagt
      301 gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc tgcttcttgt
      361 cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac tgttagcatc
      421 aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac ggttaaaaac
      481 aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc taaacgctgg
      541 tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc cccgtaagaa
      601 aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag agacaaactc
      661 cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa aaccactact
      721 ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc ctccatttta
      781 tttatattgg atagtcttta gacaagaata acattttttt gatatttcac caatcattca
      841 gctgtttcct taaagcactt gtatttcact tgatacttcg ctagaattgc aagtgatgac
      901 actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc ctttatacga
      961 ttcaaagacc taaaggaggc gagaaatgtc ccattttgga aagccattgt ctatctttta
     1021 gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt cttaaacaac
     1081 attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat ccaaaacggg
     1141 cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat tatcttcaca
     1201 tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat ggggaatttt
     1261 gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac aggcacgact
     1321 tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc tttaaaaaac
     1381 ttaacaggta agcagctccg ttctttttta agtgaagcat ctataccact ttggataaaa
     1441 gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat cactttgcta
     1501 tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc tacttttctt
     1561 gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc aacaatcttg
     1621 atggtctttc ttccttcttt tgacactagc gccttcattg cttttgcagg aatctttatt
     1681 tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat aaagacaact
     1741 cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt cacagcccct
     1801 tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa agttcttctt
     1861 gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga aagaatcatt
     1921 tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat agcgcctggt
     1981 tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat cagtttgggc
     2041 tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg ggtccaatct
     2101 atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca tattttactt
     2161 tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg aatcatcctt
     2221 gggtattctc gtggtctttt tcaagcacaa aaggcacaga gtgacatgaa atggttaggt
     2281 tcatcggtcc attaccaatc tatccaaggg aaaaaaatac taatcattgg aactggccat
     2341 attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta tggaatcaat
     2401 acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca actaaaggaa
     2461 gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga aacaactggg
     2521 ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat caatgttggc
     2581 cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca gcaactttcc
     2641 tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc tctttggaaa
     2701 atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt tcaaaagaaa
     2761 ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga gcttcaaacc
     2821 aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc aaaagaaaaa
     2881 agagaaggtg acaaaaattg tgtcaccctc tcttttttta ttgtttttta aagtgatagg
     2941 ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta aagaaccatt
     3001 catctgcttc ctcgataaaa aaaagtgtcc catcaatttc cactttcttt acaggatgtt
     3061 ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt ttgccgtaga
     3121 cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag tcaaaccact
     3181 tttgtgcttt tggcgttacc gttaatttca tcccaacaac ctactttctc ttttctttct
     3241 attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaata accagtagat
     3301 tatcattttc acgaacatat tcatctgctg caggagaagc gataaccgtt tgatctactc
     3361 ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca gctaatgttt
     3421 tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta tctgacaact
     3481 ctaggtaatc taaaatattc tttgacacta aattatgcgc aatccttatt cccatgtctc
     3541 gctcgggatg gacgacgcga tcagctccga tcttttcaag tactctggca tgatactcat
     3601 tttgggcttt tgctaaaata tttggtacgc ccatttcctt taccatcaga gtaactaaga
     3661 tactggcttg aatatcttct ccaatagcga caattacatg atcgaaattt cttatgccta
     3721 atgatcgaag tgtcatttca tcctgtgcat ttgccacaac agcatgagtg gctatattca
     3781 tatattcatt gacccgatct tcattactat caatagccag tacttcttga ccagcttcga
     3841 tcagtgttcg gcagatactt ccgccaaatc gtccaagacc tacaattgca aaattttgtt
     3901 tcaccgtttt cctcctttag tatcttcttt tctcagtata acaaagcctt tacaaaaaca
     3961 aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt ctcagctttt
     4021 gattatcctc tttatcatcg tttttatttc accaaaccat gtttgaatgc atagatagcg
     4081 gcttgtgtac ggtcttccac ttctagctta gccaatatat tggaaacatg tgtttttact
     4141 gttttcagtg taataaacaa ttcgtccgct atttcctgat tactttttcc ttcagaaatc
     4201 agcattaaaa tttcattttc ccgattcgtt agttcttcat gtaatacagg ctcatttcgc
     4261 cgactcattt tttccatcat ttttgttgtc acttctggtt ctaatactcg ctcgccacgt
     4321 tgcgttgccc ttatagcatc agcaatttca tgtgcggtag aggtcttcaa caaataccct
     4381 gctgcacctg cttcaatggc aggataaact ttttcgtcat caataaaact ggttacgata
     4441 atgattttag cttctggcca atcttttaag attgcttttg ttgattcgat accatccatt
     4501 tcttccatga ctaagtccat taaaatgacg tccggtcgaa gtgtcatcgc cttctcatag
     4561 ccttgtcgtc cattttcagc ttctccaatt acttcaatgt cttcttgaat agataaataa
     4621 gaagagacgc ctaagcgcac catttcatgg tcatctacta ataaaacttt tatcatcctt
     4681 atctatgcct ccttcatcaa agggaccttg atttctacac ttgtaccttg acctttaaaa
     4741 ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt gttcaatcca
     4801 taacttcccg ttttcgtttc attcatatta aaccctgttc cgtcgtcaat aatacgtaac
     4861 aaaagattat tgtctatttt gtgcaaatat acctctaatt cattggcttt agcatgtctt
     4921 aatgtatttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat ggaactagat
     4981 agttttacat cttctacatc ccatttcagc gaaatctgga ttttattttg caattccttc
     5041 aataactgct cgataccttg ctttaaactt ttctcttcta aattgactgg acgtagatga
     5101 agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgctac cattttcaac
     5161 tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt gctccgtcaa tgccgacatc
     5221 atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat gcagctcacg ggctaatcga
     5281 tgtctttcta attctaaaat ttcttctttg gtttgtccat ctacatattg gggacggctc
     5341 gttacaattt gtaattcact agatacttca atcatttttt cttttatctt agtgatttcc
     5401 ttatcaatat ctttgatgta taaatcgtcc gatgcacttg ggatcggaag aagcaacaat
     5461 ttggattcat aattgccaga agaaagtgca cgcagctttt cttcgatttt cccgtatttt
     5521 gtcttttgaa taagtgataa taatagaaag acaattaatc ctacacctaa ggaaatggct
     5581 aacaaatgga agataagcgg aacatataat aaacgtgcac gcagtaattc tccccaccaa
     5641 tgactttgat tgctggcata aaaataagtg aaaagtgaaa atagaataat aaggaaagcc
     5701 gcaatccccg aataaacagc tagcatcgct ctggatattt ttcccatcat attcgtatca
     5761 cctcgacatc tccaatcaag gtattagtaa taatcttcaa tctacgaggg ttttcatcat
     5821 aatcactgct gtagattttg atttgttcat ttttcaaaga aaattgctct tcttcaaaca
     5881 agacatttcc tactaatgtc gcatgctcta atcggatggc tacgcccaaa ggtactaaga
     5941 tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt ggcaaaagcg
     6001 tattgcccaa atctatgata gtatcaccag aaatgacggc gatgttgata tcgtcccatt
     6061 catagacgtc gtttccaata cgttggttac cgaataattg ctgtcttttc cgctcattat
     6121 tatgtgtttt taattgctct gtttggatca tcatgatctg tttctttcgc caaaaagcat
     6181 ttttggttaa atctatccct gaaatctcta cacctttcag accaataaat aaaacggcaa
     6241 atacagccat catccataaa gcaggactgt tgactaagct aaaaaaaatg accaaacttc
     6301 ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg tttgcgcata gctagatata
     6361 tattgaaaat accgaatatc actaaaagca aaagtccagt attgtttacg atttgccaaa
     6421 cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa acgccaagaa ctattcatcg
     6481 ctctttctcc ctcctgacac attctcattt catcatacct atttttttaa ctgttggcta
     6541 taggacttta gatgtaagat cgtttgttga aatctaaaat acacaaagag aaaaagatct
     6601 agagtgtatc atcagtaaga tgaactctct agatctttca gtcagactac gcttgttcta
     6661 ctttgacgat tttaactaac atatcgccac ctggagtagc aatagttact tcatcatttt
     6721 tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt ggaaattttg ccagaaaacg
     6781 gatctgcttc agcgcttccg acaatcgtgt actcttcttc ttctccatct ggtagttcga
     6841 tgaatgtaac tgttttacca attgatacct catctgcatc tacaccgtcg ttatcgatga
     6901 tttgagcaaa acgaatcata ttttcaattg ttgtgattct tccttcaaca aatgcttgtt
     6961 catctttcgc tgattcatat tcagagtttt cggaaagatc tccgaaaccg cgggcaattt
     7021 tgattcgttc aatgatttct ccgcgtttga ctgtttttag ttcttctaat tctttttcta
     7081 atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt aacgtctcct
     7141 tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa aaaagattac
     7201 cacgcttttc ttcaaatgta aatgtttttc taaaaatgat caatgtctag agcctgtttt
     7261 tctaagaatt atttacatat ttttccacta attccatatg ttcttcataa gttttagcga
     7321 aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt tctgctggtt
     7381 ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggtccaggt ccatagccgg
     7441 tattggtata caaattatat ggtgaatcta cagctgtatc ttcatacgtc actaattctt
     7501 tgtgttcacc taatgcgtat aaaacagaga tatctgactg aagtggcata tttgctttga
     7561 ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt ttcttttacg ccttcttttt
     7621 caactagaga agctagagtt aaaacttctt gtacagtcaa atttttctgc ttgatagctg
     7681 aataatagtt ttgcatgacc gtattcgtct tgttcaccat ttcaatcact agatccttaa
     7741 gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa gtaaccttcc aaacggtatt
     7801 ttgtgttctc agctttagat gcactttcca acagttctgg gaatttttgg tgcaactcgt
     7861 taaagaaagt ctcatcattc actaaatcta aaaattcttt cttgtcttta ccagttacct
     7921 tcgctacccg ttcagcaatc tgatcgatat cgtacccttc tggaatggca attttcccat
     7981 ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa ctgtttgcca atctcgtcaa
     8041 gagtcatatt aggcgctaac tgataatagc cagcttgaaa gccagttaaa tttttaaatt
     8101 ttgtataata attgaaaaca atcccgcttt taataatatt gtccttctcc aaaatctcac
     8161 caatctgctt gttagaagag ccactcggga tctcgacttg gactaattgg tcatctgatt
     8221 tatctaaagg cttcaacccg ctatctacat aacgatacac ggtaaatcca agaaatccgc
     8281 caataatcaa taatgtaaga gcaatcacta aaatgatctt cctcacaatc ttatcttctt
     8341 ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta ctggcttctt
     8401 ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac aactcctcct tactatcaag
     8461 ttcgtaactt tattcattat acatgatatt tgaattggcg tgaatgattt tatttaaata
     8521 ttctatcaaa aatgtaagta gtaaatccat tactataaga ttaaaataaa tgttatcaag
     8581 atacaagcta ttttagcttt attctttctg taaaaaagaa aggagactaa ataaactatt
     8641 atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat ttttaatgat cgttgtcgtg
     8701 ttgtttagcc ttagtgcgtt ctctacagga actattcttg gatggcaatt ttacaataat
     8761 gaacactctc agatagttgc ccatgctcaa tcagctcaaa aaaagttgat actgactacc
     8821 aatgaacaac agctcgttga aaaaatcgat caactattaa gaaaaaatga ttatattggc
     8881 tcaatttacg taaagaaaaa taatcgcatc atcatggaaa aaggctatgg ctatgcgaat
     8941 aaactcacgc aacaaccaaa tgatccttct ctttattatc agattggttc ggttcaaaaa
     9001 gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg ggcacctttc tttagatact
     9061 aagctggctc aattctatcc acaaattgct ggcagccagc aaattactat ccgtgacttg
     9121 ctgtatatgc gctcaggctt gcaccgaact gcttctccaa ccataccgat gactgatgaa
     9181 gaagtcgttc aatttgcgat tcagcactta aaatttacag attatcagac ctctcattat
     9241 gattcattga actttaccat attgactgga attttgatca aattaacaaa tcaaccgtat
     9301 gaaaaattat tgaccgatga tatcatcgaa cctttaggat tacagcatac aaaattttat
     9361 gaccaagtca aaaattcatc caatcatgca ctatcctatc aaatgtctaa agaggatgat
     9421 tactttaaac cactgacaga atctgaaaca gagatccata acgaattagg gactggtaat
     9481 atcagtatgt ccgtctatga tttaaatacg ctgtttacac aagttttatc tggaaaacta
     9541 gtttccaaag acttactctt ctcattatgg cgtgaaaatg cgaatggaca tccatatagt
     9601 ggtggggtat atagcggaga taattatatc ctgtcgcaag gaaatatcga ctcttttgat
     9661 acatctgcta tatttagcaa agattcacag gatgctatca tcatggaaag caatataaaa
     9721 gctgacaaaa ctatcaaact gcctactgct gatctaagaa accaaatcta tgacctgatg
     9781 gaaggaacta ctaagttggc aaatagctga tatgatgaaa agatagcaaa tactacttct
     9841 ttgaatagca tttgcttaaa aaaagtactc gactaaacgt aggaaagagt gtgggacaaa
     9901 tctaaaaatc aatttttgct acagacttta attccaaata aacagaacag aaagaggtgt
     9961 tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat tatttgacaa
    10021 agggcctatt aaatctaaaa tagtatcttt agattgctaa aatatgtgct agcaaatcat
    10081 tcatggcttg ttcttatttt taattttatg caataaaaaa gaagaacaat tagcgtgatt
    10141 atagaaataa ataatgagat tttagaaaaa aatgtgtatg aatacctgat tgttacttta
    10201 tttattccac tttcactttt tactgtaaca aaaggacctc cttgttttag tactgtagca
    10261 ttttttgaat taacagtaac cgttaatcct ttataccgga atacaggtaa aactattgtt
    10321 ttgccttttg aagatttaaa cgtaaatgta atttcatctt tatttggaga aaagctacta
    10381 taaataactt gaccatcttc tgtaacttga tggtttttca aaagcaattg atctttttta
    10441 tagcctatag gtacataatc aaaattctca aattcttcaa gtttattttc catttgttca
    10501 ttagaataag ttccttgaaa attcatttct ttcaccatgt taactactga tgaataattt
    10561 aaaattgaaa tagctaatat taataatagt ataaaatttc ttctccttgt ataagaaaat
    10621 ttttccaact gtggagaaat aaatagagat actgaaaaag atatgaacaa tgttgaaaat
    10681 atcataaaac gccaaggagc ttgaataata tttaaaggag tattttgtaa ggcaaaccat
    10741 ggaaatattt ttgtacaaag tagaatagat attataccca ttatataagt gattttagat
    10801 aagattccta attttttaaa gttaaatgtt agtataatta aacaaataag caaagttaag
    10861 ccaataccaa atgatagtaa ttctgaatct atacttccaa tcaaaaactc tttacccaga
    10921 taaattgctc ttttttgtaa tatatccaca taaggtctat tcagatcttg atacaatgtc
    10981 tgctcaatca taggacctat aaacgctagt gaaagaagta aagtaactat agcagctttt
    11041 attaatttaa taattaagaa gatttctatt ttctttttcc gataaaattg aataattaaa
    11101 gcaattataa tcattaccat agtcatagct acagaaagaa catgagtgta aattaataaa
    11161 gtcatgctaa tcgccaacat aacccaagat tctttttttg agtcgtaaag ctttataaac
    11221 ccgagtaaaa taagaggaag tattgacatt gcgagtaatt cccccattgc ccctcgaaga
    11281 aatatttcta cactcctaaa agaagatgtt gtatatataa tagcgaaaaa ggagctagta
    11341 acatgttttt tggtaatttc atacattaca taatgacata taaatagagt aatgagcgta
    11401 acaatataaa g
//
LOCUS       DACDVT010000076        11219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDVT010000076 DACDVT010000000
VERSION     DACDVT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.077"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            181..1440
                     /gene="lysA"
                     /locus_tag="IWL52_002145"
     CDS             181..1440
                     /gene="lysA"
                     /locus_tag="IWL52_002145"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9778674.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1427..2227
                     /gene="dapF"
                     /locus_tag="IWL52_002146"
     CDS             1427..2227
                     /gene="dapF"
                     /locus_tag="IWL52_002146"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9778675.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2230..3111
                     /locus_tag="IWL52_002147"
     CDS             2230..3111
                     /locus_tag="IWL52_002147"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9778676.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3108..3824
                     /locus_tag="IWL52_002148"
     CDS             3108..3824
                     /locus_tag="IWL52_002148"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9778677.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3830..4525
                     /gene="dapD"
                     /locus_tag="IWL52_002149"
     CDS             3830..4525
                     /gene="dapD"
                     /locus_tag="IWL52_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9778678.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4541..5542
                     /locus_tag="IWL52_002150"
     CDS             4541..5542
                     /locus_tag="IWL52_002150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9778679.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5539..6900
                     /locus_tag="IWL52_002151"
     CDS             5539..6900
                     /locus_tag="IWL52_002151"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9778680.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6860..8011
                     /locus_tag="IWL52_002152"
     CDS             6860..8011
                     /locus_tag="IWL52_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9778681.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7986..9155
                     /locus_tag="IWL52_002153"
     CDS             7986..9155
                     /locus_tag="IWL52_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9778682.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9330..10709
                     /locus_tag="IWL52_002154"
     CDS             9330..10709
                     /locus_tag="IWL52_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9778683.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10990..11219
                     /locus_tag="IWL52_002155"
                     /pseudo
     CDS             10990..11219
                     /locus_tag="IWL52_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3734 a         1954 c         2502 g         3029 t
ORIGIN      
        1 cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat
       61 atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca
      121 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
      181 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
      241 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
      301 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
      361 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
      421 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
      481 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
      541 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
      601 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
      661 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
      721 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
      781 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
      841 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
      901 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
      961 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
     1021 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
     1081 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     1141 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     1201 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     1261 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     1321 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     1381 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     1441 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     1501 tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt
     1561 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     1621 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     1681 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     1741 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     1801 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     1861 tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt
     1921 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     1981 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     2041 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     2101 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     2161 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga
     2221 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     2281 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     2341 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     2401 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     2461 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     2521 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     2581 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     2641 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     2701 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     2761 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     2821 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     2881 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     2941 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     3001 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     3061 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     3121 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     3181 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     3241 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     3301 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     3361 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     3421 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     3481 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     3541 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     3601 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     3661 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     3721 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     3781 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     3841 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     3901 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     3961 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     4021 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     4081 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     4141 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     4201 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     4261 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     4321 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     4381 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     4441 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     4501 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     4561 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     4621 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     4681 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     4741 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     4801 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     4861 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     4921 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     4981 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     5041 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     5101 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     5161 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     5221 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     5281 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
     5341 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
     5401 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
     5461 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
     5521 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
     5581 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
     5641 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
     5701 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
     5761 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
     5821 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
     5881 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
     5941 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
     6001 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
     6061 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
     6121 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
     6181 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
     6241 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
     6301 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
     6361 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
     6421 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
     6481 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
     6541 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
     6601 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
     6661 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
     6721 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
     6781 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
     6841 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
     6901 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga
     6961 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
     7021 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
     7081 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
     7141 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
     7201 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
     7261 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
     7321 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
     7381 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
     7441 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
     7501 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc
     7561 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
     7621 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
     7681 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
     7741 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
     7801 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
     7861 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
     7921 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
     7981 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
     8041 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
     8101 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
     8161 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
     8221 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
     8281 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
     8341 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
     8401 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
     8461 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
     8521 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
     8581 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
     8641 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
     8701 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
     8761 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
     8821 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
     8881 gaagagatta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
     8941 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
     9001 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
     9061 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
     9121 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
     9181 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
     9241 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
     9301 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
     9361 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
     9421 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
     9481 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
     9541 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
     9601 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
     9661 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
     9721 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
     9781 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
     9841 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
     9901 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
     9961 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
    10021 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
    10081 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    10141 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    10201 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    10261 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    10321 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    10381 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    10441 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    10501 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    10561 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    10621 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    10681 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    10741 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    10801 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag
    10861 ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg
    10921 gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga
    10981 ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa
    11041 atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta
    11101 agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga
    11161 ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDVT010000077        11148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDVT010000077 DACDVT010000000
VERSION     DACDVT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.078"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWL52_002156"
     CDS             6..305
                     /locus_tag="IWL52_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9778684.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWL52_002157"
     CDS             378..1739
                     /locus_tag="IWL52_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9778685.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWL52_002158"
     CDS             1761..2021
                     /locus_tag="IWL52_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778686.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWL52_002159"
     CDS             2035..2787
                     /locus_tag="IWL52_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9778687.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWL52_002160"
     CDS             complement(2881..3486)
                     /locus_tag="IWL52_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9778688.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="IWL52_002161"
     CDS             complement(3542..4516)
                     /locus_tag="IWL52_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9778689.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5673)
                     /locus_tag="IWL52_002162"
     CDS             complement(4894..5673)
                     /locus_tag="IWL52_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9778690.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPPDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IWL52_002163"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IWL52_002163"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9778691.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="IWL52_002164"
     CDS             7164..8561
                     /locus_tag="IWL52_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9778692.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="IWL52_002165"
     CDS             8642..9082
                     /locus_tag="IWL52_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9778693.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="IWL52_002166"
     CDS             9229..9555
                     /locus_tag="IWL52_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9778694.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="IWL52_002167"
     CDS             9556..10305
                     /locus_tag="IWL52_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778695.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="IWL52_002168"
     CDS             complement(10331..10741)
                     /locus_tag="IWL52_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9778696.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3536 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaatt
//
LOCUS       DACDVT010000078        11139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDVT010000078 DACDVT010000000
VERSION     DACDVT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.079"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            198..1004
                     /locus_tag="IWL52_002169"
     CDS             198..1004
                     /locus_tag="IWL52_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9778697.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1016..2236
                     /locus_tag="IWL52_002170"
     CDS             1016..2236
                     /locus_tag="IWL52_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778698.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2226..3812
                     /locus_tag="IWL52_002171"
     CDS             2226..3812
                     /locus_tag="IWL52_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9778699.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3851..5989
                     /locus_tag="IWL52_002172"
     CDS             3851..5989
                     /locus_tag="IWL52_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9778700.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(6357..7340)
                     /locus_tag="IWL52_002173"
     CDS             complement(6357..7340)
                     /locus_tag="IWL52_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9778701.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            7718..9109
                     /locus_tag="IWL52_002174"
     CDS             7718..9109
                     /locus_tag="IWL52_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9778702.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            9125..10165
                     /locus_tag="IWL52_002175"
     CDS             9125..10165
                     /locus_tag="IWL52_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9778703.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRKIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            10184..>11139
                     /locus_tag="IWL52_002176"
     CDS             10184..>11139
                     /locus_tag="IWL52_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9778704.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLD"
BASE COUNT         3774 a         1776 c         2174 g         3415 t
ORIGIN      
        1 acgagagagg ctgtgacaac cgtcgcagcc ttttttggtt tattgcaatt gtctagcatc
       61 ttctgtaaaa tagaaacgtt agagaaaatc ttgtatcata gaaaattaag aaagaaataa
      121 gaacgcagct cggtaccaaa aatatggtaa aatggcactt gactgtaatt cactgaaaat
      181 cgcttggagg aacacaaatg tttaaagatt tgtatttatt tatgaaggac atttatcaga
      241 acagaaaatt actgctacag ttttcgatca atgacttcaa atcaagatat gcaggatcgt
      301 ttttaggaat cttatgggcg tttatcaatc cgctatttac tgtattgatt tattggctgg
      361 tatttggtgt tggactgaaa gcacaaatga tcgatgcaaa atacccattt atcgtatatt
      421 tagttaccgg gatgattccc tggttcttct tctcagatgc atttgcatca actacggttg
      481 tttttcggga atatacttat ttagtaaaga aagtcgtatt caatattcgt atcttgccca
      541 cgacgaaaat cctatccaat ctgtacaccc atttattctt cattctgatt ggcatagctg
      601 tcgcgcttat aaatggtata tatccttcag caatgtcttt acagctgatt tattatttat
      661 tttgcatgtg tgctttcttg acaggattga cttggctgac ggcatcgatc caaccatttt
      721 tacctgatat catgcaatta attacgatcg tgctgcagtt gatcatgtgg acattaccga
      781 tattatggag ccctaatcag tttgattcag gtcttgtaag catattgaaa ttaaatccgc
      841 tgtactatgt tgttcaaggt tatcgtgagt catttttaag tgaagcttgg ttctgggagc
      901 atggaacata tactttgtat ttctgggttt tcacgttagt aatgttcgta atcggctctg
      961 cagtgttccg tagattgaaa ccacattttt cagatgtgct gtaaggagaa tagctatgac
     1021 agaatatgca attagactaa aagatatcac aaaatcttat aatatgtacg caaaaccttc
     1081 ggatcgattt cgtgaagccc tcaatccttt taagaaatcc tatcatgatg tattttatgc
     1141 gttgaaaaac gtgaatgtag aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa
     1201 cggatcggga aaatctacaa tgttgaaaat cattacggga gtactgacac caacatccgg
     1261 cacgatggaa atcgaaggaa aaatctcagc tttgctagaa ctaggatcag gattcaatcc
     1321 tgagtattct ggctatgaaa atatctattt aaatggaatg gttctaggat tttcaagaga
     1381 agaagtcgat gaaatggtcg atgatatcat tgaatttgct gacatcggcg accatattta
     1441 tcaacctgta aaaacctatt caagtgggat gttcgttcgt ctagcttttg ctgtagcgat
     1501 caatgtgaat cctgatattt tgattgtgga tgaagcattg gcagtaggag atcttgaatt
     1561 tcaactaaaa tgtatggaaa aatttacaga aatcaagaac tcgggaaaga cgattttatt
     1621 cgtttctcat gatattagtt cgattcgccg tttttgtgat cgttcctatt ggttgaaaaa
     1681 tggaaaagtt gtcgaatatg gtgatacgat ggaagtaacg acgaattacg aaaacttttt
     1741 gaagaaaaaa tcagttaaaa cagtagatcg aaataaaaac caagtagaac gcttcgcagc
     1801 tccagatatc gtagacgtga cgaaagccga attgttggat aaagataaaa atccagtgga
     1861 tatgctggaa caaggagaaa aactatacgt taaagtaacc tatgaagtaa aagacgatac
     1921 aatcaaaaat ccagttttag gaattgctct gcgtacagtc gacaattttt atgtgtgcgg
     1981 actcaatact ctactagatg gtatcaagat cccttggaaa aaaggtgaga atatctttta
     2041 tctggagtat gaaaagttag gtttgttagc tggtgaatat tattttgacg ttgcggtctt
     2101 tgaagaaaat gcaacggttc ctttggtata caagacaaaa tacatgaact tgtttgtcag
     2161 cggttcttat atcggagaag gcatcgtggt cttagaccat aaatgggaag aagggactca
     2221 tagcaatgaa atatgatttt gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg
     2281 ccgcacagat caaaccagga agtaaagttt tagagtttgg tcctggaaac ggccgattaa
     2341 cgaaacattt gatcggagca aaacaatgtg aagtgagcat tgtcgagcta gataaggaat
     2401 tgttcgattt tgtcagcgag tttgcccaag atggatttta tggagatatc gaaagttttg
     2461 aatgggcaaa ctattatgct ggacaaacat ttgattacgt tttgtttgcc gatgttttag
     2521 aacatttggt agatcctgga aaaacgttga agaaagttag agaatttttg aatgaagaag
     2581 gagaaattct aattacattc cctaaccttg cgcataattc tgtcatgatt gatttgttca
     2641 ataatcaatt gccatgggct tcttatggtt tgctagatga aacccataat tctttttata
     2701 ctcatgatgg cttccaaaaa gtttttgaaa aagcaggatt atacatcaat attgaagatt
     2761 atttgtactt agctgtaggt gatacagaat taaaatcaac ttatgaagaa ttgccggaag
     2821 ctgtacgtta tgatttcaaa atgcgaccat ttggcgaagt ctaccaatac ttcttctcat
     2881 tgatgaaaca tccagtagca caaagttcca ttgcgcagcc gcaaaattct aattatgtga
     2941 aagtattaga agtaatccag caaacgaaac aagatgaaat aattcaacaa ataccattca
     3001 ataatttcac aggggaaaat gagacactaa gtttccatgt atcagaatta gcagaacgaa
     3061 tggtgtttcg cttcgcgcag caacctagct ttatcgaatt cagcgcagaa gctgctggag
     3121 aaaaactcac atttatcgat tcaaatgctg tagtcaaaac agtaaatgat tgttatttgt
     3181 ttgatggtaa agatttacca gagttctttt taacggatat tgcagggaaa gaagtgacta
     3241 tccattgtca ctatcgtttt atcggagaat tgacaccaac aatgaaagaa ttgctggaaa
     3301 caatccgtcc aatggctgaa gttacaaagc agctttctga gaaaaatgat gaattagaaa
     3361 gagaaaatca ttatcttatt gagaataata caagattaga tcagacgttg aaaacaacca
     3421 taaatcgcta ttgcacgttg ttggaaagtg atgaatggac aatcaaacat agattagggc
     3481 gacgtaagaa agaaacatcg aaaaaaatcc aagaaaaaga gctttctcta tgtattgacg
     3541 aaaaaatctg ggatgcagaa acaaagatat tgaaaatcat cgggtgggga atcgctaatt
     3601 cggatcgcca accattgtcc tataagttat cagccgacca atcaccattt tttgaagcaa
     3661 ttcctgtttt acgagaagaa gtcaatcaag caaaacagct agctgaagga gcaaaagcag
     3721 gatttgaact gcggatcctt tgcgagaagg aacgttcatt cttagttgaa gcagtggctc
     3781 aaaatggtca atcatggatc attgaaatgt aaataatgat taacttataa agataaggag
     3841 taaacgaatc gtgcaaaaaa aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg
     3901 ggccactggc gatttgctta ttgtgggctg ggcaattgat gaagtgacca aagaaatccc
     3961 tacgataaag gtagaaaaag aaaatgtgat tgctgaagca acacatgtcg taagattgga
     4021 tatcaatcat ttatataatc ttgatgttaa gacccaaagc ggttttaagg tacgtttatc
     4081 agggaaaatg agaggaaaag caatcttaga cttccaaaca gcaagacatc aaaatggcat
     4141 agctgtgaaa ttaaacggta aatatcctta tgatgacggc attgaatctt catgggaaag
     4201 aaaaaaaaga ttactgaaaa aagggataaa ttatgcaagg actcatggcg taaaaaaagc
     4261 aatccgccgg gtcaaactgg aattgaaacc aggatcgatc gactatgcag agtggatcag
     4321 tcgtcatgag aaacctgatc tgaaagagca aaggaaagaa gtacaaggat ttgcctaccg
     4381 cccattgatt tccatactta tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg
     4441 cattgattcg gtacttgatc aaacgtatga tcactgggaa ctatgtatca gtgatgatgc
     4501 ttctactgat ccagctatcc gaaagtgtct agaatcctat caagcaaaag atgatagaat
     4561 taaagtcgtt ttccgacaag aaaatggaca catcagcctg gcaactaatt ctgcattgga
     4621 aatggcagaa ggagagttta tagcgctctt agataatgat gacgaattac ctccatttgc
     4681 cttatatgaa gtggcaaaag tgctaaacgt acatccagaa ctggatttga tctacagtga
     4741 cgaagataaa atcgatgcag atgggaatcg ctttgacccg cacttcaaag cagactggtc
     4801 tcccgataca ttgatgggaa ataattatat ctctcactta ggcgtatatc ggacaagcat
     4861 tgttaaggaa cttggcggtt tccgtaaagg atatgaaggc tctcaagatt atgatttggt
     4921 tcttcgtgtg acagaacaaa ttcctgcaga acacatttac cacattgacc gtgtcttgta
     4981 tcattggcgg acgatcccgg gatcaactgc aagcaatgga gaagctaaaa gctacatcta
     5041 tgattctgga gtgaaagcat tgacagatgc tctatctaga agaaatatca agggaagcgt
     5101 acatcctggc agaatttctg gtttttatga aatcacttat gatgttttgc aagaagactt
     5161 ggtcagtgtg atcattccaa ctaagaatgg ctatgaagat ctaaaaactt gtgtggattc
     5221 gatcatcgaa aaaacgtctt atccaaatta tgaaatcatt attgcagata atggaagtac
     5281 agatcctaaa atgcaagaat tatttgcaga atacaagcat cagctgaaag accgatttat
     5341 cgtcgaatta attgacattc cattcaatta ttcacgtatc aacaatctgg cagcagaaaa
     5401 agcaaacggc aaatactttt tattcttgaa taacgataca gaagtgattg aacctgactg
     5461 gatgacagcg atggtttctt atgcacagtt tgatcgaatc ggctgtgtag gagcaaaact
     5521 gttttatcca gatgatacaa cgcagcatgc aggagtatta ttaggaatcg gtggtgttgc
     5581 agggcatgca ttgaataact atgatcgaac acattgtggt tatttcggcc gattggtcat
     5641 tgacgtcaat tacttagcag tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa
     5701 tgctgttaac gggtttgatg aaacattaga agtagcattc aacgatgttg acttgtgctt
     5761 gaaagtttat gaactgggtc gttacaatgt ctatgcacat caagcagaat tgtaccattt
     5821 cgaatctaaa tcaagaggtt atgaagatac tccagaaaaa caaagacgtt ttgctggtga
     5881 aatcaaaaaa atgcaagata aatggccagc atatattgcg catgatccat tttacaatga
     5941 taatttaaca aaagaaggaa tcggcgactt ttcgttgaga ccagattaaa ctataataaa
     6001 aagctgttat gttgtgattc gctcgcacat aacggctttt tatagttttt tgtcaaaaaa
     6061 aggcagaagg tgccgactca aaaaaataag tcgaggttgg gacagaagtg tttaactccg
     6121 agaaataaga agaaattcac gaaaattgct tttcaaattt ttgtgaattt cggcttattt
     6181 aagaagaagt tgcttctgct cccaccgttt attcgttttt aaagaatgag acaaaagtgt
     6241 ttcttacttt tgtcccacgc tcttattcat gctcagagcg ccaatagata caaaatcata
     6301 gccaaagttt cacgaaccct agctgtcaaa gaatcgatat tttattttaa tataggctat
     6361 tttaaaataa atggtttaag gtattcctct acaaaagcca ctctatcaat tatcatgggt
     6421 ttaaaataag aagtgatgga gataaccaga ttgttcttag cgtcaatata aagaacattt
     6481 ccactattac caattgctga atatatttcg ttctcagaat ctaataccca ccataaatag
     6541 ccatatctca tattttgtaa gtcttctaaa gcactggttc taggtttgaa catctcgttt
     6601 atccacatgg gtggtacgat ttgttttcct ttatacatcc ctttatttag gcataataca
     6661 ccaatttttg ccagatcact tgccgataaa cacaaaccat atcctgccgt cccgatattt
     6721 agtggatcac aaaaccagac attggtttta ggagacttat ccattgtaaa ctgcttatgt
     6781 tcttcagctg tttcagcata ataattgaca tggtttggta tccctatagg atcaaataga
     6841 tacttattgg caaaatcaac agttgttagc ttacttgtct gatacaacaa gccagttaat
     6901 atgtgtaaac agacagtttg atagttgaat tcgccggtta taccttttct tcctcccaat
     6961 aaatctagtg atgtttttgt ccaatcttca cttgaacaga cttttgtcca tggatcgcct
     7021 ttacatttgt agggtgcttt cattgttagc aagtgcttga ttgtaatagc gtaaatagtt
     7081 ttctccccgc gtttcacttt atactctgga aaataatcta atactttatc atcgatactt
     7141 ttgattaatc cttgctctaa agcaatgcca atcagcaaag atacaatact tttcgttgct
     7201 gacatgatat gacaagtatc gtctttttta taaccattcc attcatctgt ataaacttct
     7261 accccatcct tatatgcgac aatttgacat atatttggtt gtcgaaaacc aataaaatca
     7321 tgcaactctt gtttattcat aagatattac ctcactttct tctataagca taatgtcatt
     7381 aaaaatcgat ttcaattttt tcgattcttc acgtaataca ggtattctta tggactagac
     7441 agcgctttgg gaagatggat tctctgaaaa atataaaggt tcactttatg agtggataaa
     7501 aaaagcagca aatacttcga cttctttaat ttaccaacat tagtgaaaat cgattcatct
     7561 ggagatttgt aatttagagt ccttgtcttg attagaggtg tataaagctt tttattagac
     7621 atttttaaat tccattacaa tttgcttata aactaggttt ttaaaggtga ctatggtaag
     7681 ataatagaga tattattttg tgattaggag tgtatatatg aataaaaatg gagaatggag
     7741 tgctgcacga cggatattca ttatattaat ggatgttatt gtatacaatg cagcaatata
     7801 tagtagtttc ttactgaagt ttcgtggaga gatcccatta cgaaatttag aaactttcca
     7861 acactcagca attattattt cagtgatttt tattgcgttg aacattttgt tgggtgcata
     7921 tgttttttat aatcgaatga tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt
     7981 gatgacacta gggatcatgg tagtgacttt tgcgggccgc tggtttgctt ttccacgtct
     8041 tgttatctta ctttcatttg ttctcggtac gcttttctta agtatctatc gtattctcgt
     8101 atataaactt tatttacgcg taagtcgaga taaaaaggtc gtcattattg ggttagaaaa
     8161 ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga
     8221 agcagtcatt cttgataact attatgcaaa catcaaaaat atgattaaag acatcgatat
     8281 tgtctatctt gcttctcata tcgaagaaac tgaaaaaatc aaaatctatc aattagttac
     8341 taaaaatgaa aaaaaattgt ttttgaatac aacatttgaa aacttgacat tgatcaatcc
     8401 aaatattatg aatttcgaag atgaaagtat tattgaagca tctggattta gaatccaacc
     8461 tgaatatgaa ctattcaaac gtttgtttga tatcatcgtt tcacttgttt tattagttat
     8521 tgcttcacca tttatgttat tcacagcaat tttagtcaaa gtgacatcac cgggaccaat
     8581 catctataaa caagtgcgga tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg
     8641 ctcgatgacc gccacagccg aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc
     8701 acgagtaacg cctgtaggta aatttattcg agctgtccgc tttgatgaat taccacaaat
     8761 cttcaatgtg ctgaaaggcg atatgtcgat cgtaggacca cgtcctgaac gtcctttctt
     8821 cgtcgatcag ttcaatgcag aaaaccctta ttactactta cggcataatg ttcgtgctgg
     8881 tatcactggg tatgcacaag tctatggaaa atatgtctct gattacaaca gtaaattacg
     8941 ttttgattta ctttatatca aaaaatattc attgatgctt gatttaaaga tcttgctgca
     9001 aaccatcaag atcctatttg ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt
     9061 gatctcattg gcagaaattg cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt
     9121 aaaaatgaac aatccgaaat tcagcataat cgtaccagta tacaatgcag aagcaactat
     9181 tggtcgaaag attgatattc taaaagaatt ggattatgaa gagtacgaag tcgttttagt
     9241 aaatgatgga tcaacagatc aaacacagga aatcattcaa gaactgattg aaaatgatga
     9301 acgttttgaa ttgattacta cgaccaatca agggcccgga ttagcaagaa acgaaggaat
     9361 aagacagtca aaaggagaat atctcttatt cttcgatgca gatgacacgc cagtaaaaac
     9421 gatcttgtca gattatgatc atttgctaga aaagagtcca aaagcggatc ttgttgttag
     9481 ttcgtttgtt tttcgtacaa tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt
     9541 tacagaatgt cactatctaa ctcacgaaga gtttataaaa gacatgtatc agttaatgaa
     9601 tgaacagttg atgtatgttg tatggaacaa atgctatcgt agcgatattc tgaaaaataa
     9661 ccaaattcta tttaaaactt ataatagttg tgaggatcga atatttaact tacattatta
     9721 tgaatactgt catcaagtga tcatgaaccc aaaagtagaa tatatttatg aatttgaagg
     9781 tgggaagggg atcactaatc aataccgacc taacaagttc gaaacattta aagaattcta
     9841 ttcattagca aatactgtaa cagacgatat taataaagac ggtatggcgg cgcttctttt
     9901 aaaagggaca acttcagtta ttttttctat ttatagtacc tctgcaagaa cttcaaaaga
     9961 gaagaaagac gaagcaaagc agatattgag tgatccagtg atccgagaag ccaagcaaat
    10021 tgctaagacg gattcttcag caaaaaaaat aacaaagttg ctttacaata tgccagctcc
    10081 actattttta tctgctgtaa aagttggaag cttcgttgaa gtgaaaatgc cagggttaat
    10141 ggctgctttg aagcgagtat attagttaat ggaggaaaat ataatgaaaa gaattttgca
    10201 ttttcaggga cgaatgggtt taggtggagc agagtctttc atgatgaatt tatatcgtaa
    10261 aattgatcgt acaaactatc aatttgattt tttgatttat gaagattatg cagatgttca
    10321 agactatcat tcagagattg aaagattagg aggccgtatt tttgttgtac ctaatcctaa
    10381 gaaaaatatt tttaaatatt taatagaagt gaataagtta ctaaaaaaag aatctttttc
    10441 tatagttcac aatcaggttt attttggcgg gggaattaat ttatggttag ccaaaaagaa
    10501 tggaatcaga cagagaattg ctcatagcca tgcaactgaa gatggaaaat ctcaaaatat
    10561 tgtaatgaat gtattgcgta aatttttaac caaattgttg ttacaaaatg caacagacta
    10621 ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa aaccatccct ttgagatagt
    10681 tcataatggt attgatttag agctttatag taaaaattca gaagctaagg ttaataagag
    10741 aaaagaattg gatatctcaa tgagtacttt tgttgtggga aacattggac gattagagaa
    10801 acaaaaaaat caatcttatt tgttagagat ttttttagaa atactaaaaa tgaattctga
    10861 ttcttacttg attttagtag gtgaagggtc acttagaaat aaactgaaag ccaaagcagt
    10921 agaattggat atagagaaaa gagtaaaatt tttaggagaa agaaacgata ttcccgagtt
    10981 attatctgtg atggatgttt ttgtaatgcc ttctctttat gaaggtctgc caatggttgg
    11041 attagaagca caagcagcaa aattaaaatt agtcttagca gatactattt ccaaagatac
    11101 aaagttaaca aataatgtgg attttgtatc attagatga
//
LOCUS       DACDVT010000079        11041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDVT010000079 DACDVT010000000
VERSION     DACDVT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.080"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..977
                     /gene="gnd"
                     /locus_tag="IWL52_002177"
     CDS             75..977
                     /gene="gnd"
                     /locus_tag="IWL52_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9778705.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            979..1980
                     /locus_tag="IWL52_002178"
     CDS             979..1980
                     /locus_tag="IWL52_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9778706.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1977..2261
                     /locus_tag="IWL52_002179"
     CDS             1977..2261
                     /locus_tag="IWL52_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9778707.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2294..3091
                     /locus_tag="IWL52_002180"
     CDS             2294..3091
                     /locus_tag="IWL52_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9778708.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3105..3938
                     /locus_tag="IWL52_002181"
     CDS             3105..3938
                     /locus_tag="IWL52_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9778709.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3956..4813
                     /locus_tag="IWL52_002182"
     CDS             3956..4813
                     /locus_tag="IWL52_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9778710.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4835..5344
                     /locus_tag="IWL52_002183"
     CDS             4835..5344
                     /locus_tag="IWL52_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9778711.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5381..5782
                     /locus_tag="IWL52_002184"
     CDS             5381..5782
                     /locus_tag="IWL52_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778712.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5894..6247
                     /locus_tag="IWL52_002185"
     CDS             5894..6247
                     /locus_tag="IWL52_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778713.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6281..6724
                     /locus_tag="IWL52_002186"
     CDS             6281..6724
                     /locus_tag="IWL52_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9778714.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6741..7826
                     /gene="uxuA"
                     /locus_tag="IWL52_002187"
     CDS             6741..7826
                     /gene="uxuA"
                     /locus_tag="IWL52_002187"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9778715.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7819..8532
                     /locus_tag="IWL52_002188"
     CDS             7819..8532
                     /locus_tag="IWL52_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9778716.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            9284..9484
                     /locus_tag="IWL52_002189"
     CDS             9284..9484
                     /locus_tag="IWL52_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9778717.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(9588..10889)
                     /locus_tag="IWL52_002190"
     CDS             complement(9588..10889)
                     /locus_tag="IWL52_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9778718.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3644 a         1769 c         2419 g         3209 t
ORIGIN      
        1 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
       61 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
      121 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
      181 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
      241 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
      301 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
      361 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
      421 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
      481 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
      541 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
      601 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
      661 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
      721 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
      781 ggggaatcgt ttctgcatca ggtgaagcaa aacggacggt tgaaacagcg ctagaaatgg
      841 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
      901 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
      961 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
     1021 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
     1081 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
     1141 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
     1201 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
     1261 caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat
     1321 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
     1381 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
     1441 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
     1501 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
     1561 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
     1621 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
     1681 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
     1741 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
     1801 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
     1861 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
     1921 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
     1981 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
     2041 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
     2101 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
     2161 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
     2221 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
     2281 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
     2341 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
     2401 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
     2461 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
     2521 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
     2581 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
     2641 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
     2701 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgtggc
     2761 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
     2821 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
     2881 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
     2941 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
     3001 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
     3061 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
     3121 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
     3181 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
     3241 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
     3301 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
     3361 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
     3421 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
     3481 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
     3541 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
     3601 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
     3661 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
     3721 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
     3781 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
     3841 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
     3901 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
     3961 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
     4021 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
     4081 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
     4141 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
     4201 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
     4261 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
     4321 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
     4381 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
     4441 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
     4501 ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg aaatgctatc
     4561 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
     4621 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
     4681 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
     4741 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
     4801 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
     4861 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
     4921 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
     4981 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
     5041 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
     5101 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
     5161 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
     5221 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
     5281 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
     5341 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
     5401 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
     5461 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
     5521 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
     5581 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
     5641 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
     5701 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
     5761 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
     5821 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
     5881 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
     5941 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
     6001 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
     6061 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
     6121 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
     6181 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
     6241 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
     6301 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
     6361 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
     6421 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
     6481 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
     6541 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
     6601 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
     6661 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
     6721 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
     6781 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
     6841 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
     6901 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
     6961 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
     7021 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
     7081 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
     7141 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
     7201 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
     7261 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
     7321 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
     7381 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
     7441 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
     7501 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
     7561 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
     7621 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
     7681 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
     7741 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
     7801 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
     7861 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
     7921 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
     7981 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
     8041 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
     8101 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
     8161 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
     8221 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
     8281 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
     8341 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
     8401 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
     8461 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
     8521 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
     8581 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
     8641 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
     8701 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
     8761 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
     8821 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
     8881 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
     8941 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
     9001 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
     9061 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
     9121 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
     9181 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
     9241 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
     9301 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
     9361 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
     9421 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
     9481 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gggctctttg tcaaatagga
     9541 ctgatagagt gagataagaa catctaaggt agaattcctt ttctacttta gatgtttttt
     9601 gattcatagg taatcagatt atttagtaag aaaattctgg tcttcatttt ccgaaagctt
     9661 cgaaagccat aagcgactcg tttcaatgct ttgatgtggg tatgcagatt ttctattttg
     9721 gcatttgaat aaggcaattc gagggctaaa cgaatgccgg attcatagtt tataaaagct
     9781 ttttttatag aatgcagttc ttttggaacg gaattgggca tcgttcttac aagtttaaag
     9841 aaagtgtccg cctctttgtc gtgaaacgct tgaagaattt cttgatagta aaaataggcg
     9901 attcgtaatt cgtcagagat cgaaagcaga cgatccacca cttcactttc ggtgagatac
     9961 gcccaaccaa aattgcgcca tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg
    10021 atcagaagcc gagacaacgc ctttaattgt cgataatatt tggcttgtgt tggatcgttt
    10081 cgatcaaagc ttttcatgat tcgtacgcga atatcctcaa atttacgaat gagatgtttg
    10141 acaatgtgaa aacgatccac aacgagtttt gcctttggaa aacattcctt tatcagagag
    10201 gcataagaaa cattcatgtc cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca
    10261 aattgcataa aataagaaac gagagccttt ttcttccgtg aaggaagaat atccagtaat
    10321 tttccagtat cgccatccat acaagaaaag gagtagggac cgacttgatt tttcagtgaa
    10381 cgaaattcat ccattaataa acaacgcggt aaccaatttc tttttacttt tgtcatgggg
    10441 atcagagaag cgagtgtccg agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt
    10501 gtcgtcattg cttgtttttc agttagctct tctaaaattt tatagcgcac ttgtttagcg
    10561 ataaagcagt ttctatcgac aatgtaggta ttcgccgtaa aataggtgtg gcagccacga
    10621 cagtggtagc gttgtttcgc taaagcaaga taagtcagtt ggttcgcaca tttattaagt
    10681 tgaatgagcg attttttctt tccgttctta acgatcatgc cttcatgatt catctgttta
    10741 cacaaaggac agcgacgaat ttctttactt aacgtcgcag aaacaacaag actcttgata
    10801 ttcttaatgc tcttcgattc cacatttgtg atgaaaatgt ttttatcttt gattccaagt
    10861 agtagtttga tatgatgaag atcaggcata cagaacgctc ctttgattgt gttgtggtga
    10921 ttcaattcta acggagcttc tgtatgtttt gttttttcat gctcaaaagg gaccgataga
    10981 atgattttct atcagtccta gaaattatag agccaagaaa agacggtttt tttgtacgaa
    11041 a
//
LOCUS       DACDVT010000080        10749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDVT010000080 DACDVT010000000
VERSION     DACDVT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.081"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(23..1807)
                     /gene="pyk"
                     /locus_tag="IWL52_002191"
     CDS             complement(23..1807)
                     /gene="pyk"
                     /locus_tag="IWL52_002191"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9778719.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1881..2843)
                     /gene="pfkA"
                     /locus_tag="IWL52_002192"
     CDS             complement(1881..2843)
                     /gene="pfkA"
                     /locus_tag="IWL52_002192"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9778720.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3011..6325)
                     /gene="dnaE"
                     /locus_tag="IWL52_002193"
     CDS             complement(3011..6325)
                     /gene="dnaE"
                     /locus_tag="IWL52_002193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9778721.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            6556..6741
                     /locus_tag="IWL52_002194"
     CDS             6556..6741
                     /locus_tag="IWL52_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9778722.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6803..7999)
                     /locus_tag="IWL52_002195"
     CDS             complement(6803..7999)
                     /locus_tag="IWL52_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9778723.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8169..9605)
                     /locus_tag="IWL52_002196"
     CDS             complement(8169..9605)
                     /locus_tag="IWL52_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9778724.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9898..10707
                     /locus_tag="IWL52_002197"
     CDS             9898..10707
                     /locus_tag="IWL52_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9778725.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3208 a         2249 c         1918 g         3374 t
ORIGIN      
        1 ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa acgatgccgc
       61 gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg cctttagcac
      121 caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa gtcaatccgc
      181 cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct ttgtcagttg
      241 atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca gcagatgaag
      301 caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct aataatttag
      361 aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact ggtacaccag
      421 cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct tttttagctg
      481 ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg taaacgcccc
      541 agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag atatctgcat
      601 ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca gcaacgattg
      661 ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt tcagttacat
      721 ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca ttttcagctt
      781 cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg tttgcagatt
      841 caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg tcagaagctt
      901 ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta gcagtgataa
      961 ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt ggtactagtt
     1021 cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca gaaactttga
     1081 tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag atttgaacat
     1141 gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt gctttacgta
     1201 caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata tcgtcagcat
     1261 cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc cctttacgtg
     1321 atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct tcgtcttttt
     1381 caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca acgtgaacat
     1441 cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca tgttcaggat
     1501 ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca cgaccaaaat
     1561 cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta tctaaaagaa
     1621 taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata cgtgataatt
     1681 gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca gattcaatca
     1741 attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg attttggttt
     1801 ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac acagcttaaa
     1861 tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata tagtgataag
     1921 tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac gatttcgttg
     1981 tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac agcgtaagca
     2041 ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg gacaacgtgt
     2101 cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc agcgaattca
     2161 tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc gcgatcgcgg
     2221 ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg gatgatgatt
     2281 tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt gcgtcccatt
     2341 acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg gtcaattgat
     2401 tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc aggaatatca
     2461 ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa agccattgcg
     2521 ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa ttttttcaat
     2581 tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg tgcagaatac
     2641 aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag acgacgaata
     2701 tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag tccttcgtaa
     2761 atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc tccgccactg
     2821 gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact ttatcctaag
     2881 tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta acattatttt
     2941 tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa gtagtatttt
     3001 ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata agttgccgct
     3061 ttatactctt gctgtcatcc acccaataag catctgacag aactactttt cgttgcgttt
     3121 tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc acttgtaatt
     3181 gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa gtttgtgaag
     3241 gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt tgcagttcct
     3301 gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca acggcaaaca
     3361 aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct ccactactgt
     3421 cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct ctaacttcct
     3481 tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc agctgaactt
     3541 gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa taaataccta
     3601 agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact tcttcttctt
     3661 ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata ttttggattt
     3721 ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca aaaactccta
     3781 ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc cgtatgagga
     3841 attgatccac tgactggaac gggccatttt gttttctttc gtttaatata tcttctacaa
     3901 aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc ttattgacca
     3961 actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt ttatttttac
     4021 gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa ttcatcaggc
     4081 tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat cccacgaatg
     4141 aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg atatattcat
     4201 atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat acaaaatgcg
     4261 acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc agaatatctg
     4321 actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt tcctgataga
     4381 cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg tagcgaatgg
     4441 cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg cctggtcgat
     4501 acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt cgtaaaacgt
     4561 tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt tgaaacaaga
     4621 gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac ccttctactc
     4681 gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct aaaaagtcca
     4741 ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga aaagcttcat
     4801 cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata actacacctg
     4861 ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt ttgaataaaa
     4921 gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt ttataagctt
     4981 gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt gcttcacttt
     5041 gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc attgtgccaa
     5101 atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa ttcaatactt
     5161 cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag cgttcggggt
     5221 ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg atttctaaca
     5281 cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg attttgtttc
     5341 tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat cccatttgat
     5401 ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag tcagtgattc
     5461 ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt tttccattag
     5521 gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa atcgattcag
     5581 ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat cgtttgatca
     5641 tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca gataaatttt
     5701 ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca aaaagttctt
     5761 cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct tttatcacat
     5821 actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata taaaaatttc
     5881 tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc tctttattga
     5941 aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg gtttctgtca
     6001 tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc atcagctgct
     6061 gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct tcataagtat
     6121 aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata cgatagaatt
     6181 caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag ccttgttttt
     6241 ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa taagaagtaa
     6301 ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt aaaattcact
     6361 agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga agttgattat
     6421 tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag aactcaaatt
     6481 ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa ttaagataga
     6541 aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt tctaggtcaa
     6601 gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac tttgacaaca
     6661 atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc gccaagaaaa
     6721 gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca agctcttttt
     6781 tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga aagttgaata
     6841 aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca gcactgtaat
     6901 tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca aagttgctaa
     6961 acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc ttactgatca
     7021 gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag cgaagtatac
     7081 ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat gataaaatga
     7141 gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct agtttcggtg
     7201 aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca aaaaggatat
     7261 tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg atcgtagtga
     7321 ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag agatgatcca
     7381 tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga tggaaatctt
     7441 ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct gtgatcaaga
     7501 atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt ccgatcagat
     7561 ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt tctctaggtg
     7621 cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc ccattccaaa
     7681 accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc attgttaatg
     7741 tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca gctagatttc
     7801 cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct agtcccgaaa
     7861 acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt atgaatggca
     7921 taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc actaaatttt
     7981 ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata cctattttcg
     8041 aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg tcattttata
     8101 tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt ccaatccctt
     8161 ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat atcaacgatc
     8221 cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat tgtatagaaa
     8281 ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa gatcgatgca
     8341 aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa agaagccatc
     8401 atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac aatggcgatc
     8461 agtggagaga ataaactact taatgcgaat agcactgcca ctactactga agtcaaacca
     8521 gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt attcgatgta
     8581 ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt atccattttt
     8641 gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa aatacctgtg
     8701 cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa actgaatgcg
     8761 atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga ttgcatgcct
     8821 tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt acctaacgaa
     8881 ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc aaggactgtg
     8941 gtcgctacga taccgataag aatagcccca cggacatttt ttacaactaa aatggctgtc
     9001 agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc taacgcaggg
     9061 acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac agagctcgaa
     9121 atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc gacatacgca
     9181 acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat cgcccgaata
     9241 atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca aataaacacc
     9301 attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa agtgaaaaat
     9361 gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa tcccattacc
     9421 agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa gggcatacct
     9481 gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa gaatgtcgtt
     9541 actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc gaaaaacttt
     9601 tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat aataaaaaat
     9661 aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt cattcgcttt
     9721 tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt tagcttatgt
     9781 ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt gctcttgtta
     9841 attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg acccttattg
     9901 aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga ttcgatgatt
     9961 acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca tgtcagtatt
    10021 gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga attaacaaca
    10081 ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg ggatggtgaa
    10141 agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa aaagcagcta
    10201 aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag ttttgatttc
    10261 tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct ttctcctaaa
    10321 aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt tgcaggagct
    10381 gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac atggggtgga
    10441 cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg cgtgcaagag
    10501 attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga cgagcataat
    10561 gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg tactgaccaa
    10621 ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg tttagctgtt
    10681 tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa agaggttgtg
    10741 acaattttt
//
LOCUS       DACDVT010000081        10497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDVT010000081 DACDVT010000000
VERSION     DACDVT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.082"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..196)
                     /locus_tag="IWL52_002198"
     rRNA            complement(<1..196)
                     /locus_tag="IWL52_002198"
                     /product="16S ribosomal RNA"
     gene            116..259
                     /locus_tag="IWL52_002199"
     CDS             116..259
                     /locus_tag="IWL52_002199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778726.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(877..2373)
                     /gene="lysS"
                     /locus_tag="IWL52_002200"
     CDS             complement(877..2373)
                     /gene="lysS"
                     /locus_tag="IWL52_002200"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9778727.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2484..3488)
                     /gene="dusB"
                     /locus_tag="IWL52_002201"
     CDS             complement(2484..3488)
                     /gene="dusB"
                     /locus_tag="IWL52_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9778728.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3489..4376)
                     /gene="hslO"
                     /locus_tag="IWL52_002202"
     CDS             complement(3489..4376)
                     /gene="hslO"
                     /locus_tag="IWL52_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9778729.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4593..6704)
                     /gene="hflB"
                     /locus_tag="IWL52_002203"
     CDS             complement(4593..6704)
                     /gene="hflB"
                     /locus_tag="IWL52_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9778730.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6794..7339)
                     /gene="hpt"
                     /locus_tag="IWL52_002204"
     CDS             complement(6794..7339)
                     /gene="hpt"
                     /locus_tag="IWL52_002204"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9778731.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7339..8784)
                     /gene="tilS"
                     /locus_tag="IWL52_002205"
     CDS             complement(7339..8784)
                     /gene="tilS"
                     /locus_tag="IWL52_002205"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9778732.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8904..9374)
                     /locus_tag="IWL52_002206"
     CDS             complement(8904..9374)
                     /locus_tag="IWL52_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9778733.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9420..9845)
                     /locus_tag="IWL52_002207"
     CDS             complement(9420..9845)
                     /locus_tag="IWL52_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9778734.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9912..10181)
                     /locus_tag="IWL52_002208"
     CDS             complement(9912..10181)
                     /locus_tag="IWL52_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9778735.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(10192..>10497)
                     /locus_tag="IWL52_002209"
     CDS             complement(10192..>10497)
                     /locus_tag="IWL52_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9778736.1"
                     /translation="FVRIETKELNAFWKERHFGRKLMFSLLCMTVVLLLYYGVLNIVF
                     GPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLFTVREWLMLPFGKKILRLNKKIKK"
BASE COUNT         2667 a         2324 c         1807 g         3699 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactct tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcgagag tgtttcccga
      361 ttcatttttg gctgccgtag caacttttat atcatatcag agagacaagc gattgtcaac
      421 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcggcaac tcatttatca
      481 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      541 ttcgcaagtg gttctttttt aacgactcag ttatcttatc atgtcgttag taacatgtca
      601 ataacttttt tgttatgttt ttgaataaat attttcttct gtcttttgga caagtaataa
      661 tataccaact aaatttagct tggtcaacga attttttgta ttttttcaag tttttttcaa
      721 taaacgatta ttcataactt ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta
      781 gaatagtcgg cattagcttt tgaactttcg ttattattta ttcctttcta cacagcaaaa
      841 gctgcctaag gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa
      901 aacatcccga attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc
      961 tagaccacca gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc
     1021 gtgagcttcg tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg
     1081 atcaattgga tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag
     1141 ttcgaatcga tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc
     1201 gactggatgt ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa
     1261 gaattcattg ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc
     1321 ggctaaagct aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc
     1381 tttgattgca tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc
     1441 atcatatgtg atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc
     1501 tgtcagattc attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa
     1561 ttctggatta tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac
     1621 tttttccatt ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata
     1681 taaatcaatg tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc
     1741 ctcattgtgc aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg
     1801 gatttcgctg ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat
     1861 taaatccaag tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc
     1921 tggcagtgga cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc
     1981 gcctgtgtct gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg
     2041 tttgaatact tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg
     2101 gccttcacga tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat
     2161 acgaccagct acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc
     2221 aaatagttca tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc
     2281 gattccatct tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa
     2341 atcttctact tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc
     2401 tctttcctat aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg
     2461 aaaattcacg aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt
     2521 tctacaaatt gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct
     2581 actttcactt tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat
     2641 tctctacaag ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct
     2701 gtagcaattt tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc
     2761 tgtgtgcggt gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct
     2821 gcaccgacat agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc
     2881 ataaatggga tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct
     2941 tcatacatct gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt
     3001 gctgctaatg cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg
     3061 attgttactg ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca
     3121 ggatctaaaa gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc
     3181 atattgatat ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact
     3241 agactatctt tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg
     3301 tggagcatct ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca
     3361 catacgacta agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt
     3421 ccagccatcg gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg
     3481 tgttccactt aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta
     3541 tacttgtatt tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc
     3601 attgcttgga tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa
     3661 cagtcacatt tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc
     3721 aatcctttca atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt
     3781 gtcgttttta attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt
     3841 tggatcatga atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt
     3901 ccaacagctg aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt
     3961 tctccagaaa caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg
     4021 acagtaaaca ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt
     4081 agaggaagac tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca
     4141 tctactacaa ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct
     4201 cctttgagcg tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag
     4261 gaggtgtgcc aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg
     4321 gcgtatgctc ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt
     4381 tatgtactcc tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt
     4441 ttcaactttg gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca
     4501 ccctatttac aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg
     4561 gagtcatatc agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta
     4621 tcttctgatt gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt
     4681 tttgcttctt caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa
     4741 gcacgttttg cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca
     4801 gtaccttcag gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt
     4861 tcgaattcca aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt
     4921 tctcgagctt tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa
     4981 gcgacttgtt ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg
     5041 tttccttcat attgaacagg acctaaacga tcactcattc catattctgt taccatgctt
     5101 cgtgctagag ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag
     5161 atgatttctt cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct
     5221 ttcgtcatca agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg
     5281 cgagggatga tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc
     5341 gtatgaccag cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct
     5401 tttttagctg gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt
     5461 ttctttttat ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct
     5521 gctccggcaa aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc
     5581 ggtttattcc gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca
     5641 actaaaatct ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa
     5701 cggttggttg cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca
     5761 agcaattggt tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt
     5821 tgacgaccta ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct
     5881 gtttcaaaca aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca
     5941 gaacccgaga tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag
     6001 gttttacctg ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat
     6061 tcaacaaatc ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct
     6121 tcttccgcac ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg
     6181 gcttttgatt taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt
     6241 cccatcatca tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg
     6301 gaaacccata taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt
     6361 gccagttctg ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg
     6421 gtagtagtcg tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc
     6481 tgttctttgt attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct
     6541 ttgatcttgc ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc
     6601 tggccgttat taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca
     6661 taatacaagg catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag
     6721 cgtcaattta atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa
     6781 ttgagataca gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa
     6841 ggttacgata tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt
     6901 tgaagccaac ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg
     6961 taacaatttt gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg
     7021 ttcttccgct gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat
     7081 ctaagtcttt gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa
     7141 catccatgaa atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg
     7201 gtaccgctcc ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta
     7261 actctttgcc tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct
     7321 caatatcctt ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag
     7381 aagtatgtag tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg
     7441 tatcaagcta tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga
     7501 attaggtact ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa
     7561 tttaatgcgg tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca
     7621 aatgtcatga gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg
     7681 tttgaccaga ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa
     7741 caagcgctgg tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa
     7801 atgatactct cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt
     7861 ctgttgccac tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt
     7921 tgagaatgct ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc
     7981 ttcaatcatt tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat
     8041 caactgtccc atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg
     8101 ctggaaatgt ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg
     8161 aagccgattt ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg
     8221 cgattccgct gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag
     8281 tctgcccgta gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc
     8341 acgaacgatt ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa
     8401 cgtgtcatat tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc
     8461 ttctgtttct atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt
     8521 cttctcttca caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg
     8581 gtgattgatg tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa
     8641 atggagcaaa accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg
     8701 ttgccagaat ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt
     8761 tttcccgcct ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc
     8821 gggaaaacgt ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc
     8881 gcactatcat tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct
     8941 tctgtgtttc gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc
     9001 aatgaatcga agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt
     9061 gctggacgat ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct
     9121 tctccagatt cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca
     9181 tcatttactg aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg
     9241 tcttttacat agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct
     9301 agatcaataa acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct
     9361 acctcgattg acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct
     9421 tattttgcag ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt
     9481 gattctaata taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca
     9541 agtttagcta catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg
     9601 tctgcgttga cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc
     9661 atcctttgca tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca
     9721 acaaaaattg cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat
     9781 tcagcatact ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc
     9841 gtcacttgga ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa
     9901 gcttgtctaa tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt
     9961 ttttgtgcat cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt
    10021 ttgttgccaa actggacttt caaaatatcg cctactttga catcactgga agatttcgcc
    10081 aatttaccgt tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt
    10141 ttgatgattc gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt
    10201 aattttttta tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac
    10261 tgtaaacaga cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc
    10321 tatcacgctg acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa
    10381 tacaccataa tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg
    10441 tccaaaatgc cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaa
//
LOCUS       DACDVT010000082         9933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDVT010000082 DACDVT010000000
VERSION     DACDVT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.084"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            72..506
                     /locus_tag="IWL52_002210"
     CDS             72..506
                     /locus_tag="IWL52_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9778737.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="IWL52_002211"
     CDS             complement(584..1117)
                     /locus_tag="IWL52_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9778738.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IWL52_002212"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IWL52_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9778739.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IWL52_002213"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IWL52_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9778740.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="IWL52_002214"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="IWL52_002214"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9778741.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="IWL52_002215"
     CDS             complement(4842..5420)
                     /locus_tag="IWL52_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9778742.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="IWL52_002216"
     CDS             5742..6212
                     /locus_tag="IWL52_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9778743.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="IWL52_002217"
     CDS             complement(6320..7546)
                     /locus_tag="IWL52_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9778744.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="IWL52_002218"
     CDS             complement(8300..8500)
                     /locus_tag="IWL52_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9778745.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="IWL52_002219"
     CDS             complement(8634..9242)
                     /locus_tag="IWL52_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9778746.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="IWL52_002220"
     CDS             complement(9327..9797)
                     /locus_tag="IWL52_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9778747.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         2960 a         1978 c         1648 g         3347 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac cat
//
LOCUS       DACDVT010000083         9916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDVT010000083 DACDVT010000000
VERSION     DACDVT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.085"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            276..761
                     /locus_tag="IWL52_002221"
     CDS             276..761
                     /locus_tag="IWL52_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9778748.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            754..1437
                     /locus_tag="IWL52_002222"
     CDS             754..1437
                     /locus_tag="IWL52_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9778749.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1563..4208
                     /gene="polA"
                     /locus_tag="IWL52_002223"
     CDS             1563..4208
                     /gene="polA"
                     /locus_tag="IWL52_002223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9778750.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4253..5089
                     /gene="mutM"
                     /locus_tag="IWL52_002224"
     CDS             4253..5089
                     /gene="mutM"
                     /locus_tag="IWL52_002224"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9778751.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5053..5682
                     /locus_tag="IWL52_002225"
     CDS             5053..5682
                     /locus_tag="IWL52_002225"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9778752.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5734..6066
                     /locus_tag="IWL52_002226"
     CDS             5734..6066
                     /locus_tag="IWL52_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9778753.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6378..6869
                     /gene="nrdR"
                     /locus_tag="IWL52_002227"
     CDS             6378..6869
                     /gene="nrdR"
                     /locus_tag="IWL52_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9778754.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6892..8265
                     /locus_tag="IWL52_002228"
     CDS             6892..8265
                     /locus_tag="IWL52_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9778755.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8265..9191
                     /gene="dnaI"
                     /locus_tag="IWL52_002229"
     CDS             8265..9191
                     /gene="dnaI"
                     /locus_tag="IWL52_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9778756.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9320..9760
                     /locus_tag="IWL52_002230"
     CDS             9320..9760
                     /locus_tag="IWL52_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9778757.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         3418 a         1737 c         2184 g         2577 t
ORIGIN      
        1 aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta tgataaacgg
       61 tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat gagaagctgt
      121 tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc tctgtaaact
      181 ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt tggtacaata
      241 atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag ccgagaaaac
      301 taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca gaatctattg
      361 aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct ttgtatatcc
      421 aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca gtgatgctga
      481 tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca catgttgtca
      541 atttttctgt caattttatt gaaccggttt tccattatga aacactgact tttcagtttg
      601 aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa gcggagaata
      661 cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa gttcctgaat
      721 tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac atcaatgtca
      781 ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact tgcccttagt
      841 gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac ttttatcaat
      901 aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt aatggttcta
      961 ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc ctactctctt
     1021 ttgaatggca taacattagc ggttactctt gcatactacg atattggaac agtaacgcaa
     1081 gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt aacaacaaaa
     1141 agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt gatcattgca
     1201 ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat ctccctactg
     1261 ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg taatatctat
     1321 gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt gaagctgtat
     1381 ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa caactaaaaa
     1441 agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc gctttttctt
     1501 ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa ggagttaaga
     1561 agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt cgcgcattct
     1621 ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat acaaatgcaa
     1681 tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg actcatgttc
     1741 tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg gagtacaaag
     1801 gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata cgtgatttgt
     1861 tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat gatattatcg
     1921 gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt tcaggtgatc
     1981 gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta aaaggtgtca
     2041 gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg ttgacgccaa
     2101 aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc cccggtgtga
     2161 ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca gtcgaaggaa
     2221 tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg ataaatgaca
     2281 aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg cctgtggatg
     2341 tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt ccgttttata
     2401 aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa gaagtaaaaa
     2461 tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt actacggata
     2521 tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc gtcggagtag
     2581 cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc gaaaaccgag
     2641 cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat gcgaaacgaa
     2701 cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt gatgtcttgc
     2761 taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga atagctgctc
     2821 attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg gcgaaaaaag
     2881 gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta gctgcaatca
     2941 atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa gatcttttcc
     3001 ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc gggatcaaag
     3061 tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta agagagatcg
     3121 aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca aagcaattag
     3181 gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa actggatatt
     3241 ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta gaggatatcc
     3301 tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg ctgaaagtga
     3361 tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa acaggacgat
     3421 taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa gggagaaaga
     3481 tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct gactattcac
     3541 aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa gctgccttcc
     3601 tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc gaaaaggcag
     3661 aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt atcgtgtatg
     3721 gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca gcacagcaat
     3781 acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa agaatcgtac
     3841 gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga tatctgccag
     3901 acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct atcaatacac
     3961 caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg gataaacgat
     4021 taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa cttgtcttcg
     4081 aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt atggaacaag
     4141 ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg tggtacgaag
     4201 caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa aaatgccaga
     4261 attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag gaaaaacgat
     4321 tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac ccatctttga
     4381 ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt ttttaatttt
     4441 tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat atcaatttac
     4501 gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg aggatggaag
     4561 ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg aaaaaggagc
     4621 aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag atagcttctt
     4681 attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc cattgctgct
     4741 agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat tatgggaagc
     4801 gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag aacaattacg
     4861 tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca cgattcgcag
     4921 ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg tctatcagca
     4981 aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag tggcacaaag
     5041 ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg atttgtttta
     5101 ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt tcagtctcat
     5161 ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc tggaacgctt
     5221 gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga tgggcaacta
     5281 gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca aaaacttgat
     5341 cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc tctaagttcc
     5401 aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta tgaccactat
     5461 atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg tttgatgaaa
     5521 cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact tccaatcgaa
     5581 gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga aaaacttgtg
     5641 ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt gtcaattttg
     5701 ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa tcaaaaggat
     5761 aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga aaatctaatc
     5821 gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta ccaagcaaga
     5881 atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga tttgactcaa
     5941 gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag tgaccacatt
     6001 cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat cgaagagaag
     6061 ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg ttcatctgct
     6121 tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc tcagtaatca
     6181 aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat tacgcatgaa
     6241 agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg tttgtgattt
     6301 gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat aaaatgcgat
     6361 gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca cgagtaatcg
     6421 acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt gagaattgtt
     6481 cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta atcaagaaaa
     6541 acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc cgttcagcag
     6601 aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc gaaaaccgcg
     6661 tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat gtgatggaag
     6721 atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat cgccagttca
     6781 aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca aaagaaaata
     6841 ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc cttggaaaat
     6901 gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc tccgctgaga
     6961 gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg agaagccttg
     7021 agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg tcctgaaggg
     7081 atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta tgatgcaaga
     7141 aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag tgatctggga
     7201 cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt caaagactct
     7261 gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa gcttacccaa
     7321 ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa aaaattttta
     7381 gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat cgaatcgctg
     7441 aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc gacattcgat
     7501 tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga tgatccaatc
     7561 atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac acttggcgaa
     7621 ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga actgcaaaaa
     7681 gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt tgcggtttct
     7741 gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct tcagctgata
     7801 aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc agaaaaaggc
     7861 ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca atcaggtctt
     7921 tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa taatgctagc
     7981 ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa aatcgccaca
     8041 gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca atcaaaacaa
     8101 aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa gcttcctgat
     8161 tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc agagattgac
     8221 cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa gacgtaggca
     8281 aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct gaaatgatcg
     8341 ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa gaagaattga
     8401 cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa gaaaagcgta
     8461 aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag ctgacgttga
     8521 attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca cggcaaagac
     8581 aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt caatcggctt
     8641 cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa gccctagatt
     8701 ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat ctagtaggaa
     8761 gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta gcgattgctg
     8821 gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa caggcaatcg
     8881 gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt ttgatgatcg
     8941 atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc ggtgttatcc
     9001 ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt acaatggatg
     9061 aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta aaagcgaaac
     9121 ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt cgcaaccgac
     9181 gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa aagcaggtca
     9241 gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt aatgcagaaa
     9301 cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag ttggtctatt
     9361 ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg attcgattga
     9421 tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca tatttgtcgg
     9481 aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt tgaaactcgt
     9541 cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca atgacggagt
     9601 gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga aaatcggtga
     9661 caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc caccgcaagt
     9721 tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat tttttcaaaa
     9781 ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat atgggaattt
     9841 tttggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctga gtcagaatga
     9901 accacattct ggctca
//
LOCUS       DACDVT010000084         9873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDVT010000084 DACDVT010000000
VERSION     DACDVT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.086"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(96..2216)
                     /locus_tag="IWL52_002231"
     CDS             complement(96..2216)
                     /locus_tag="IWL52_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9778758.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2446..3624
                     /locus_tag="IWL52_002232"
     CDS             2446..3624
                     /locus_tag="IWL52_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9778759.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3640..4398
                     /gene="aroD"
                     /locus_tag="IWL52_002233"
     CDS             3640..4398
                     /gene="aroD"
                     /locus_tag="IWL52_002233"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9778760.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4421..4906
                     /gene="ybaK"
                     /locus_tag="IWL52_002234"
     CDS             4421..4906
                     /gene="ybaK"
                     /locus_tag="IWL52_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9778761.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4977..6230)
                     /locus_tag="IWL52_002235"
     CDS             complement(4977..6230)
                     /locus_tag="IWL52_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9778762.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6347..7696
                     /gene="gdhA"
                     /locus_tag="IWL52_002236"
     CDS             6347..7696
                     /gene="gdhA"
                     /locus_tag="IWL52_002236"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9778763.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7808..9154
                     /locus_tag="IWL52_002237"
     CDS             7808..9154
                     /locus_tag="IWL52_002237"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9778764.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9462..9848)
                     /locus_tag="IWL52_002238"
     CDS             complement(9462..9848)
                     /locus_tag="IWL52_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9778765.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3148 a         1793 c         2023 g         2909 t
ORIGIN      
        1 ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg agcagcgaca
       61 ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact tgtggtcgtt
      121 ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg tccgttgtgt
      181 tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt taatttctct
      241 aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact attcgtatag
      301 aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt atcaactttt
      361 tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat agcttcacca
      421 gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac aaaatcacca
      481 tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc taactgaata
      541 taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc aacttgtccg
      601 ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat ataagggaca
      661 cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc aactaattca
      721 gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag gacgatgact
      781 gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac ggcggtatct
      841 aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt ggctaacggg
      901 aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac agcaatgaat
      961 tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg gaaaaatggt
     1021 ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata agaagaatca
     1081 aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa tgtaccagcg
     1141 ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc aggtgcagct
     1201 tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa gccaaacaat
     1261 gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg atggaaaaag
     1321 ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg tgtaacttca
     1381 tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg gaagctttct
     1441 aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc atttggtgct
     1501 gcataatgtt cttttacata attttctact tctttcatat cattgggact tgcttctgcc
     1561 cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc attcaagcca
     1621 agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg acgatcagct
     1681 tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat cgcaaaagcc
     1741 attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag cagaacagtc
     1801 aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg aatggcactt
     1861 tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc
     1921 gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag aaaataaatg
     1981 accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat tccaagtaat
     2041 aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc actttcgatt
     2101 ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa aacgactagt
     2161 aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca tttcaagata
     2221 atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta cacagctcac
     2281 ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca gaagtttatg
     2341 ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga caagtcctat
     2401 gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa agtaaaagta
     2461 aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa agaagacctt
     2521 gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca aaaccaattt
     2581 atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt gagccagcag
     2641 aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga
     2701 actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg agaaggagac
     2761 gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc ttggtataac
     2821 gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat ctacccggaa
     2881 atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga gagccagttc
     2941 atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc
     3001 gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga agtacgggga
     3061 tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag ttataccggc
     3121 gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt cgatctagca
     3181 aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga tgtcgatcag
     3241 caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag aaaacaattt
     3301 ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa aaaagtcttt
     3361 cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt agcagaagac
     3421 gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg
     3481 attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt atatcgtctg
     3541 ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa agtgtttgtt
     3601 tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt aaagggattg
     3661 aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt ccgagaggaa
     3721 gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga atggcgagtc
     3781 gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt tcttatccat
     3841 gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga aggcggagaa
     3901 caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca cacaggatta
     3961 gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag actgatagag
     4021 gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa agaaacacca
     4081 gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc cgatatcgga
     4141 aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga gttgactaga
     4201 cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa tcttggtaaa
     4261 atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc cttaagcagc
     4321 gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat tattcaatta
     4381 ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga aaaaaatagt
     4441 aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag agcacgaata
     4501 tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc cagaaagcca
     4561 atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc ttgttgctgt
     4621 gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag ggaataaaaa
     4681 agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac gtggtggctg
     4741 ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg cagaaagcta
     4801 tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag cacctcaaga
     4861 tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca tacgtataaa
     4921 agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt tatacattat
     4981 gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc tgtgatatta
     5041 aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag accatcaatg
     5101 attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc gccagttaca
     5161 aatgatttct caataggaaa agttttgttg attgatagct taaactcttt gcatagttga
     5221 acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc catttcaagc
     5281 cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc attgaccaag
     5341 ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg tttggtttgt
     5401 gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa ttctttattg
     5461 acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa
     5521 tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa agtgatgtct
     5581 tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga tatcaagggg
     5641 gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat cttgtaccca
     5701 tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat aatggcagga
     5761 gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat
     5821 ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg attgagcaat
     5881 gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg atcctcttct
     5941 tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt ccattgcgca
     6001 aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc atcaacagga
     6061 cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt tcctgctttt
     6121 ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc
     6181 atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat tccctcacct
     6241 caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga
     6301 gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga caaacgcaac
     6361 agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc aagaagaatt
     6421 tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca ctcatcccga
     6481 atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta ttcaatttcg
     6541 agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc gtatccaata
     6601 caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg tcaatctaag
     6661 tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg gtctgccaat
     6721 cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata atgagatcat
     6781 gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat cgattgatgt
     6841 tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg gcgaatataa
     6901 acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt tttggggaag
     6961 taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac atctattgaa
     7021 cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc
     7081 catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat gttcagattc
     7141 tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga
     7201 aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta tttatcatgc
     7261 tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat gtgctactca
     7321 aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca aaatcgtagc
     7381 agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg aagcaggcgt
     7441 gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg ctttagaaat
     7501 gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc agctggatca
     7561 aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa
     7621 aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag caatggcagc
     7681 agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt ttgtttattt
     7741 gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc tataaggagg
     7801 agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg tcaacgaaca
     7861 agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac gggaaggtac
     7921 tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg acaaagaaga
     7981 atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag tattagttgt
     8041 tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct tgaatcacac
     8101 attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct ttgccggaaa
     8161 ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc gcgacttttc
     8221 agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt tccgtgtatt
     8281 caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta tctacgctac
     8341 aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt gggaaacttt
     8401 cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg gtttgttacc
     8461 aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa
     8521 agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag caatgcgcaa
     8581 cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac caggaatgca
     8641 atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag accaaaaagg
     8701 catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac aattcatcca
     8761 agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat
     8821 cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag gaaaagaagt
     8881 ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag acggtgacgt
     8941 gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt atacaatgta
     9001 cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc cattcgacca
     9061 accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac caggctttga
     9121 agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc tagatttaaa
     9181 taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt taggtactca
     9241 aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt cacagtccct
     9301 tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga ctttaagaac
     9361 agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca gtaagcaaaa
     9421 aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag gcctaatttt
     9481 tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat gatccgcttt
     9541 tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt ggcagagtca
     9601 ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc ataagcatct
     9661 acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat atcagtttct
     9721 gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt agactgatag
     9781 tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa ccctaaaatt
     9841 tttttcatga aagatctcct tttttatttt tat
//
LOCUS       DACDVT010000085         9779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDVT010000085 DACDVT010000000
VERSION     DACDVT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.087"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IWL52_002239"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IWL52_002239"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9778766.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IWL52_002240"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IWL52_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9778767.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IWL52_002241"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IWL52_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9778768.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IWL52_002242"
     CDS             2857..3732
                     /locus_tag="IWL52_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9778769.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWL52_002243"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWL52_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9778770.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IWL52_002244"
     CDS             4629..5063
                     /locus_tag="IWL52_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9778771.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IWL52_002245"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IWL52_002245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9778772.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IWL52_002246"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IWL52_002246"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9778773.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT         3427 a         1688 c         2192 g         2472 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaatac
//
LOCUS       DACDVT010000086         9664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDVT010000086 DACDVT010000000
VERSION     DACDVT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.088"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            182..1057
                     /locus_tag="IWL52_002247"
     CDS             182..1057
                     /locus_tag="IWL52_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9778774.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1161..1925
                     /locus_tag="IWL52_002248"
     CDS             1161..1925
                     /locus_tag="IWL52_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9778775.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1942..3354
                     /locus_tag="IWL52_002249"
     CDS             1942..3354
                     /locus_tag="IWL52_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9778776.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            3371..4695
                     /locus_tag="IWL52_002250"
                     /pseudo
     CDS             3371..4695
                     /locus_tag="IWL52_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4708..5166
                     /locus_tag="IWL52_002251"
     CDS             4708..5166
                     /locus_tag="IWL52_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9778777.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            5458..6759
                     /locus_tag="IWL52_002252"
     CDS             5458..6759
                     /locus_tag="IWL52_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9778778.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            6786..7046
                     /locus_tag="IWL52_002253"
     CDS             6786..7046
                     /locus_tag="IWL52_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9778779.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(8045..9280)
                     /locus_tag="IWL52_002254"
     CDS             complement(8045..9280)
                     /locus_tag="IWL52_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9778780.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3074 a         1462 c         1999 g         3129 t
ORIGIN      
        1 caattcgata caagtattat cataaaacga gtaaaaaaga tattgataac gcttttatta
       61 tgtgttaaac tatttttgta aattatgtag ggtgctacta ttcaattagg cacgacctaa
      121 gaaagggttc tttcttaggt cgttttttgt ctttaaaaat tgtttggaga caaaaatatg
      181 tatgaaggag tctgcaagtt gttttgtttt atcatatagt ggaggtgaat taacggtgta
      241 tgtgatgaaa aagctgaatc ataatgctgt attgatcgat gacgatggaa tagaaaaaat
      301 tgcgatgggg aaagggattg gcttttctgc taaagtgagt aagccatttg atgttgagga
      361 tgctgacaaa ttatttgtat tagattctaa agaaaagatc aaacgattca gtgagatagt
      421 ctcgcagatt ccaatcgaat ttatggattt tgcggaagaa gtgattgaac tgataacagc
      481 taggatctcc tctccattag attcaaatat ctatattaca ttgacagatc atatttattt
      541 tgctattcaa cgaaaaagag aagatagaga tgtcacagca attatgttgc cagaaatgag
      601 attcctttat ccaaatgaat atgatatagc aatagaagtg gtcgagcgaa tcaatcatcg
      661 atatcaaacc gagttagggt caaatgaagt aggattcatc actatgcata tcattaatgc
      721 tgagctagga gagcctaata gtttaaagag cttaaaaata gtagaaatga cgaccacgat
      781 tttgtcccat ctagaaaaat cctattcaaa cgtttatgat aaagaatcac tgacttatca
      841 tcgcttaatg gttcatatca aattcctggt taaacgcttg atttatcagg aagaaacgcc
      901 agtagaaaat attggttttt ttactgaaac atttaaacat agcaaagcgt attgtacagc
      961 actaaagatt aaagatatca tcgaacagca atttcaactt gccattcaag aaaatgaaat
     1021 catctatttg gcaattcatc tagcacgaat ctattaaaat aagggcgtaa ctatctatag
     1081 gcgcaaccaa atgacacata gtaaatggac tatgattatt tggttgcgcc tattttagta
     1141 gaaaaaagaa ggagaatgaa atgggaaaag tgatttttaa ttcggatgat tttggctata
     1201 gccatggtgt gaattatggg attatggatg cttatcagcg aggaattttg acatcaacta
     1261 cattgatggc aaatatgccg ggtttcgagc acgctgtgaa actaagaaag gaaatgccta
     1321 ggctaggtgt aggagtccat ttaacattga cgtgtggcaa accacttctc aaaactgtcg
     1381 atactcttat cgaaagcgga gagttcagat ccttatctta ttatcagcat ccatttaaca
     1441 ttgacaaaga tcaactgtat caggaatgga atgctcagat tcaaaaaatt tatcatgcgg
     1501 ggatcatacc aactcattta gacagtcatc atcatactca tactttggat gaaaaccagg
     1561 aggtagttgt tacattagca aaaaaatatg atttgccggt tcggagcaac tttgaacgaa
     1621 aagatgaagt tcgtcatgtc gattattttg agccttgttt cgatgtcgta ggagaaactg
     1681 atgaaaaaaa acgacaaatc aatacgagtg tggaagaata tttggcaagt ttactcatga
     1741 tattgagaga agataaaacg atcgaagtca tgtgtcacgc agcgtatttg gatcaggaat
     1801 tgttaaatgg ttcaagtttt gtgtatcctc gtactcacca agtcgaattt ttaattcatt
     1861 cagcatttgc tcaaaaagtg aaaaatgatc ctttgattga gttagtaaca tatgaggata
     1921 tttaatagag gaggaataaa aatgaatgtt aaatcaaatg tgcagaagct tggaagagcg
     1981 atgctacttc cagttgcagc gatgccatta gcgggattga tcatgcgttt atcagctgat
     2041 gatatgctga acatcccagt aattggtgca gctggaaatg ccgtttttgg aaacttggat
     2101 atcttatttg ctataggagt tgcaatcggt tttgtcaaaa ataaggacaa aggaatacct
     2161 gcacttactg gttttttagc gattgctacc ttgaaaaaag gcttagaaat catggaccca
     2221 gaagtgaata tggggatttt tggtggaatt atttccggtt tagtagcggc atggacgtat
     2281 aatcgcttta aagaacaaaa attaccagta gttttttctt attttgcggg cgaaaaattc
     2341 ccattgacta tggtgatgat tctacaaaca atcgcagcta tttttttggg ctttatctgg
     2401 ccgctcatcc aaattgggat tgataatttt gcaaaactgc tcgttgatat gggggcatta
     2461 ggtgtgggga tatttatgtt tttgaatcgt ttgctgatcc cctttggact gcaccatgtg
     2521 ttgaatacgt atatttatta cgatttagga agctatacat ctcctagcaa ggaagttttc
     2581 agaggagaga tgacacgctt tatcaacgga gatcctcagg ctggactgtt tttatcaggt
     2641 ttttttgtag taatgatgtt tggtatacca gctatatgtc tagctatcta taaagcagct
     2701 tataaagaaa ataaagagat ggtcaaagga atcatgggaa gcggtgcagg aacttcattt
     2761 attgcaaata tcactgagcc tgttgaattt agtttcatgt ttctttctcc tatgttgtac
     2821 gtgatccatg cactttttgc cggtttagca gggattgtct gttacttatt caatatacga
     2881 attggtttta cattcggagc atgtattgtt gattatctca tcaattttcg aatcgctacc
     2941 aatgctattt tgattcttcc aatcggaatt ttcttttttg ccttgtatta cgttactttt
     3001 tattatttga tcaataagag aaacattcag acattaggac gtgaagctaa ggcagagttt
     3061 ggcaatgaag taaccttaga agaaacagag ttagggttag caagtaaaaa ttatgaatat
     3121 atggcaacaa aaatgctaca agcgtttgga ggaaaagcca atatcctgga tgtctatagt
     3181 tgtaatacac gcctacgggt agaggtagtg gatcccacca tggtcgagga acagcgaatc
     3241 aagcaattag ggataagcgg gatcattaaa ccaactgaaa aaaattatca aattatcatc
     3301 ggcttagaag tgacttatgt tatggcagaa ttcaataaat tattagaaga atagaaagga
     3361 aaagatgatg atgaaaaaac agattatcac tatagctgga ggcgggagta cgtatacacc
     3421 aggaatcgtt caagccattt tgaacaatga aaaacggttg ccattatctg aaatacgttt
     3481 atatgatatt gatgaagaaa gaaatatgga tatgtacttg attgttaaat ttatgctgaa
     3541 aaaaaaggtt tttcaaatat tcgcattcgt gcaacggatg atccaaagct tgcttttaca
     3601 ggatgcgatt ttgttttctc acaaattcgt gttggaggat tggagatgcg agaaaaggac
     3661 gaaaagattc cacttaaaca tggattagta ggacaggaaa cgtgtggact cggaggattt
     3721 gcttatggga tgcgctcaat caaaggttta ttggaaattg tcgatcatat acaagactat
     3781 gcccccaaag catggatttt gaattataca aatccagagt cgatcgtttc tgaagctgtt
     3841 cgtcgtaaat atccgaatgt aaaaatgatt aatgcctgtg atatgactat ttcaattgaa
     3901 gaaacgattg cagtgaatta cggttatgat cgtaagaatt ggatcgttac gtattatggt
     3961 ttaaaccact ttggctggta tacatcgatt tatgataaag aattgcaacg agaaattatg
     4021 ccagagatta ttgaaaaact aataacaaaa gaaatgcaag tcgccgattt taatatagga
     4081 gacaaaactt ggcaaaaaac ctttcaaatg atgtcagtca tcacgaaaaa ttttccaagt
     4141 aatatcccga ataattactt agagtattac ttatatcctg atatggtcgt tgagcatact
     4201 gacaaagagt atacacgtgc taatatggtc atggatggac gagagaaaaa taccaaagaa
     4261 atggctgata aaatacgccg cggtattgtg gaagaagtat tgaactttaa ttttggggag
     4321 catggacaat atatcgtcga tatagctatt tccttgttga atgatgatcg tcgtcgcttt
     4381 atgctaatcg ttcctaatca aggggcgatt ccgaatctac ggtcagatgc agttgttgaa
     4441 ataccggcat atgttggtgc aactggtgtg gagacaatta cactaagaaa accaatcagt
     4501 gattttcata aaggattgat ggaggcacaa gttgcagcag agaagttact agtagatgct
     4561 tactttgaag gttcttatca aaaagcttta caagcattta cattgaatca aaccgtacca
     4621 aatgccagag tcgcaaaaaa aatactagat gaaatgatca aagtaaacaa aaaatattgg
     4681 ccagcattaa agtagaggtg ataaaaagtg catttcttta aaacaagaaa cattctaaaa
     4741 gctgtagcta gtggtcaaat tattccatta acagcagtaa aagacgaggt cttttcttca
     4801 aaaatgatag gagttggata cgctataacg aatcatgatg gatatatcca tgcgccagtt
     4861 aaaggaataa tcgataatat atttcctacc cgacatgcca taacgatcga aagtgaatct
     4921 ggcagaaaaa tactgatcca tatgggttta gatacagttg aactcgaagg aagtccgttt
     4981 cagatacggg ttgaaagtaa ccaggaggta aaagaaggga cactgttagc tattatagat
     5041 ttgaagaagt tggagcaagc acaaaaagat acggtgatca ttgtagtttc tccaggcatt
     5101 tttgaaggaa atttattaaa acaaaaccaa gaagtaaaga ttaaagatag ttgttttgaa
     5161 ttttagtgta cgatgaaaaa agttaaatag ggtgatattt atgggtgaat ttacaaaatt
     5221 tttttgaaat gataagtttc aatcactaga tttaatcatt ttgatttttg ttaattatcg
     5281 aatttttttg ttgctcctat gatttagaaa acttattttc aggattctta aaaataaatt
     5341 tgattattta agagaatata ttgaaataaa atttcttaat tatataatta gttatgaaca
     5401 ttctttgcgt tgtcatgcaa tgaatggttc atattttatt gaaaaggagt ggttttagtg
     5461 gaaaagctga tgaaatgggt cgaagagaaa cttgtcccac caatggcaaa aattggtaca
     5521 cagcgtcatt tattagcaat tcgtaatggg gttgtttcaa cattgtcatt gattttgatt
     5581 gggacatttt ttatggtctt tatcaattta ccgtttcctg gatggaatga atttattgcc
     5641 ccttattcgg caacgattgt tttacctttt aggatgacga tgggattgat ggctatttat
     5701 gcaacttttg ttatgggttc ggatctagct aaatcatatg gattagatag tgtgacaggt
     5761 ggtatattat ctttaggaac gttctttatg ttacaagttc ctgtaaacgt tttaactcct
     5821 gaaaaggcac ctcttggctg ggtcttacct atgagttctt tgggagcatc tgggatgttt
     5881 gcggggatct tatctatgat ttttgcagtg gaagtatatc gtttctttaa aaatcgaaat
     5941 attactatca aaatgccaga acaagtgcca cctgctgttg cccgtagttt tgaggcattg
     6001 attccaggtg cagtcatttt aacaattact tggctgattc gaagcgtcgc gggctttgat
     6061 gtaaacgcta ccttattatc gttattcgag cctttaacca atattttagg gaataatttg
     6121 cttggtgttt tactaccaat gtttttgatc catttgttgt ggtcatttgg tatccatggc
     6181 atgagtattg taggggctgt tgttcgtcca atgtggctga tcatgttgga tgagaatgca
     6241 aaggcattgg cagatggtac agctgccacc caattaccct atatcgctcc tgaacagttt
     6301 tatcaatgga cggtaacaat tggtggtgca ggagcaacga tcgtggtatc tgttttgttt
     6361 atattcttct gtaaatcaaa attcttgaaa gaggttggtc gtttttctat tattcctggt
     6421 atcttcaata tcaatgagcc catgattttt ggagctccta tgatgttgaa tccttacatg
     6481 ttcatcccgt tcaacttagt tcctttagtt ttgactatta tcagttattt tgcagtcaaa
     6541 ttagaaatgg tcaatagatt tactgttttg ccaacttgga cattaccagc tccaatcggc
     6601 ggttatcttt cagcaggaaa tgactggcgt gttgttatct tgatcatgat caacacattg
     6661 atagcattta ttatctatta tccatttgta aaagcttatg ataaaaagat gctgtcagat
     6721 gaacaaagtc gtggagaaaa cgaagtccaa actgtctaag tttggttttt agaaggtggg
     6781 aaacaatgta ttggctgatt ttattttttg tttttatctt tttattaacg gctagtcacc
     6841 ttatattgaa tatgctagct acatatcata ttcaaatcaa tcgatggatt tgggcactgg
     6901 catcattttt gatcgtgatt cttccaaaga tcattgtacc gcatatgaat gttttgtttt
     6961 catgggggac atatgtactt tgtggaattt ttgctatcaa ttttatgatc gagcagcata
     7021 gatggtttgt cacatcgaag ctttaaatgt tctaaaagtg gttgtgacag aagtggttag
     7081 cttcaagaaa taagaagaaa tttacgaaaa ttgcttctta aatttttgtg aaatgttggc
     7141 ttatttttcg tttggttgaa cttattaccg cagatgcaag cttttgacca ccgtttattc
     7201 ggaatatggg actgtgacaa ggcttttgtc acagcccttt tttacataaa gattattatt
     7261 gttttaactg ttttggcaga aagaaggagg caaaaatgaa aattttatca gcaatggatt
     7321 aagaattagt cgatctacta ccaaagtgat caaggcaaat atagatttgg gagcttacaa
     7381 ccatatagta ggggctgcag tatggtgtat gaatggtaat atgtatgatg agagtgttta
     7441 ttcaaatcat gaaacgtgta tagaaataat tgcgctggga atagcaatca gtcatggtga
     7501 acgtgacttt tagtttatcg ttgctgacga catagaacat tctgacatga tctactcagc
     7561 atgtggggat tgctggcagt ttatatagga gtatgcatcg gattgcgaga tcattgtctc
     7621 aacagaagag ggaggttgat tatcaaagat aaagtggtaa atcctacatt tgatttagta
     7681 attgttgaag ggactagcag cacaaatctc tacaaagcga tgcttctaga tctatatgac
     7741 acattttaag aaaatgtatt tgcctaatta ttatggtctt tcttttgaag aaacgttgag
     7801 atgatccaac cagagaaagg tgtcaaagaa tttgactaag aataaatggc agacgctacc
     7861 tatgctcatt tggattgcgt cagatctttt attttaagga tgattttgtg ttttttagac
     7921 accgtaattt aagtaactct aacttttcaa tccactattt actttttttg tattttcact
     7981 gtctgggtct cgaatagcga aattactgat gagtctctat taaaaaagct ttttctactt
     8041 gaaactatat tgggtggttg ctcaggaagg tttcgatttc ttgaattgct tgttgtggac
     8101 catttattgg aaaaacggct tgcagcgtgc gtgcctgctg acgaatcttg ggtgaagaaa
     8161 cttcgcgcag agcaatctgc agttttcttt cagtcactgg ttttgtgagg gcgattgcgt
     8221 ttccgtattt tacacaatag tcaatattac agctttgctc aaattgcatc cccacaccga
     8281 taaacggttt gccggattca caggcagttt ggacggtccc ttccccgccg tggataaaag
     8341 aaaaatcaat gaattcatgc aaaagatgac tagacagata ttctgttatt aaaagatctt
     8401 ctgagtcaga ttgaatgtca tcaagcagat agtcaatagg tgcaataacc gctagatccg
     8461 gttgtttttg taagaagaac aaaattttgc gtaacatacg cggattgcct gaactaccca
     8521 ttgcgaaata gataatacgt tttctttctt gttgctttcg ctgtataaaa gttagcactt
     8581 cttcaggcaa ggaagtactg agcttagcaa aaattggacc aacgattcga taattttccg
     8641 gcagctgttc ttcttccaaa aatagtttag gcgttgtgat gagatttaga tcagcatcca
     8701 tcatttgtaa acttgttttt ggtaaaggaa gcatttcttt ttgtgcagcg tgtttgaagg
     8761 cattggggat ccatcgggta tttactatac gcttttttgt ccagtgccaa aggggattgc
     8821 ttaaagggcc gaagaaacgt aactcgtggg gcatcagcgc atgctctgat tgaaaaaaag
     8881 gttcactcaa agcatagggt ttaacatata ctaaaggaat ctttttattg cgggccgata
     8941 aaaaaattgt cacattcgaa cctgtcacaa tcagtcgagg ctcaaactta gcaatcagac
     9001 tctgttcact agtgatacga gaggcaacca tttctttggt aaaagggttt ttaagtgtac
     9061 gcaactggtc aaattttatg atttgttctt cttgtttttt cgtaaggatc ggcgctagta
     9121 aatgatactc aaagccactt tctgtgatca aaggagcaaa agttttagaa aagccaaaga
     9181 atacacagta ataacggtct ttcatagctt tggcaacctc aatcattcgt gttgtctcag
     9241 ctaaattgaa tactgcagga gcaaaaagaa ttgtcttcat gaaatcctcc ttattggaat
     9301 gagtctatcc cttcgtttct tactagtata ctaaaaaaca cagcgttcga gtagcctatg
     9361 tatattttga atattaaact gtctctcgct gttttcacaa cttctaaatt taaattttgt
     9421 aatcaagttg aaactccaat tttcaaaaaa acacgcaaca attttttgat cgatttatat
     9481 gttgtgccag ataaatacga gaagttgtgt cattaaaaat aaacatgaga acataaaaaa
     9541 taaaataatt atattaattg ataggtgtgc tgtcgtcatt atacacacgt aagaaagaaa
     9601 tagttatgtt taaaaatatt cttagacttt ctcgccatga caattttcac tatgtgttat
     9661 aact
//
LOCUS       DACDVT010000087         9646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDVT010000087 DACDVT010000000
VERSION     DACDVT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.089"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(70..684)
                     /locus_tag="IWL52_002255"
     CDS             complement(70..684)
                     /locus_tag="IWL52_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9778781.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(831..1514)
                     /locus_tag="IWL52_002256"
     CDS             complement(831..1514)
                     /locus_tag="IWL52_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9778782.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1623..2081)
                     /locus_tag="IWL52_002257"
     CDS             complement(1623..2081)
                     /locus_tag="IWL52_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9778783.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2165..3928)
                     /locus_tag="IWL52_002258"
     CDS             complement(2165..3928)
                     /locus_tag="IWL52_002258"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9778784.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4149..5609)
                     /locus_tag="IWL52_002259"
     CDS             complement(4149..5609)
                     /locus_tag="IWL52_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778785.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5817..6743)
                     /gene="glsA"
                     /locus_tag="IWL52_002260"
     CDS             complement(5817..6743)
                     /gene="glsA"
                     /locus_tag="IWL52_002260"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9778786.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6766..8193)
                     /locus_tag="IWL52_002261"
     CDS             complement(6766..8193)
                     /locus_tag="IWL52_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9778787.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8224..9438)
                     /locus_tag="IWL52_002262"
     CDS             complement(8224..9438)
                     /locus_tag="IWL52_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9778788.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2855 a         2010 c         1602 g         3179 t
ORIGIN      
        1 ttcttttttc atttcttttg aatctgtcga tggtttatag cttattttta acataactag
       61 tgctcctttt tattcgggac gtgcaatcgt gaaaatttct cctattgctg cataatcatt
      121 tcctgccaaa cggcttacag cattcaattt tcttggatcg atccttcctg tcttatcatc
      181 atagactgtt tcatttaaat gatatttctg tacttctagc aaaaataaat cagctgttac
      241 tttattttta ttccgtatct cgacatgttc atataaggat gtttcaaaac gaacttttgc
      301 ttctgtcaat gctggtactg aaatgattac tgaatcagtt aaagtcaagt cagtcaggta
      361 cagttcgctt tcctcattag gcagattggc tgctgtttta tttacttctt ctacgttgtc
      421 ctcatcaagg atatggataa cagcttctct tgtctcaaga atattctttg ctgtgtcttt
      481 tactgcatct tgttttctct ggatcgccaa ggataagatg ggtcgattcg aactgacgat
      541 attgaagtaa ctgaagggtg cgatattcac tatatctttt gctgatttag tcgttactac
      601 tgctactggt ctagggatga ctgaaccaat cagcaacttg taattttcac gttctgtcaa
      661 ttctgaagga ctccgttcaa acatggtttc gccttctttc tataaaattc gttaaaaagc
      721 agatcttgaa gacaaaatga aaagtaatgc ttttttcatc ttcttagaat ctgctttaaa
      781 ctatctatca atttttcttt tttgtcaaga atctgtgccg tgcgtgtttt ttagttattt
      841 gctttacttg cggcttcgac atatctttta gtgatcaatt gcggacgggt gataatagat
      901 ccaactacga ctgaaaaagc acctaattca tatgctttct ctacttgttc aggagagtca
      961 gtatgtcctt ccataattac tgggatatcg acttgttcta tgatttgatt caataaatca
     1021 aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc ctgctaaagt cgtagacaaa
     1081 agatcaaaac catcttcttg cgcttgaagc gcttcctcaa aagtcgaaat atctgccata
     1141 gctaaccgac cgtgttcgtg aatcatttgg acaagttcag cagttgtttt ttctgtcgga
     1201 cgtttacgag aagttgcatc cagtgcaatg atttctgctt gcgtctccaa aagttcttcc
     1261 acttcttttt ctgtcgcagt aataaacact tctgacccat cgtaatctct tttgataatc
     1321 cctattaccg gtaaatcaac gattcgcttg atttcattga tatcttctgt cgtatttgct
     1381 cgaataccaa ctgctccgcc ttcttttgca gccaaagcca ttctccccat aataaaagaa
     1441 ctgtgtagcg gctcgttttc caaagcttga caagaaacga cgactccgcc tttgatttgt
     1501 tccagtgtat ccacaatatc tcctccagta atgttatata aaaatacagc ttcatataaa
     1561 aatgtaaccc ttttctattc aggagacaat aatgagctat ttctttcagc agacctttct
     1621 tactaaaaga aaattacttc aaattcttcg tatacgacca gcttctcttc tgtaaattct
     1681 atgttcaacg cttctttata agcatgttta aaaaactcag catggacttt ccgccaataa
     1741 gctaatgtcc gatccccttc tccttctttc caagcatgct ctttcgtgac ttcactaaaa
     1801 ggcaagatgg tcacatttat atttttgatg atacaaactg gttcattttt tccattcagg
     1861 accacgtcta attgaccaac ttttggtaaa ggctcattct cttgtttata cagatcgtaa
     1921 ccacttgatg taccagtctt tatctttttt acaactagtt cgctcaactc gtctgccatt
     1981 ttttctgtat ccccaaatga aaagaaacct aatttttctg attgataact aggatatttt
     2041 tcttggaact ttttgacata ttcttctact tgctgataca taacatccac ccctttgctt
     2101 tttataatga aaaaaccatc cagatactag tataaacaat tatctggatg gtgagcataa
     2161 tgaattattg aatattcaag acacgcagac tatcttcaac gatttgtttt ggtgttaaat
     2221 gataacgttc ataaagctgc cctacaggaa cgctatccgt gaattctttc tctgcaccaa
     2281 aatttaatac gttcattgaa gaatcaccat aataacgact gatattttcg ccaaatcctc
     2341 ctgacaaact accatcttct aatgtgatga ctaattcgtg tgtttctttt aatttatcta
     2401 atgtttctac gtcattttct gtgatcgttc ctggattgat caacgttgct tcgattcctt
     2461 tttttctaaa agcttttact gtttcttttc ctaattcaag aaaaccgcct aaagctaaaa
     2521 ttgctacttt tcctccatta tttactgttt gataggctgg aatactataa tcatttgcga
     2581 tagctgtacc atgttctaca ccatgtgtag gaattcggat tgctacaggc tgcgctgttt
     2641 gttcgaacgc ccaatctagc atagagatca tatcctcttt tgttgctgga gcaagatatt
     2701 tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt tccttggtgt gttcggtcac
     2761 tagctgagat cgttccgcca ccgactaaaa taatcactgg attctcgttg atcgctaaat
     2821 catgtgataa ttgatcatat gcccgttgca agaaagtact tgattggaag acgactggac
     2881 gtactccctg agaagccaaa gcagaagcga aagtgataga gtgctgttca gcgatcccca
     2941 cgtcaaaata atgatctgga tgtttttctt ggaatgtttt cagtccaaag gcgcctggta
     3001 tcgctgcatt gactgcggca ataggagctg tacttgtttg cattttctta tccaaataat
     3061 ccatgatgat catattaaat gtttctccag aagttacttg ctttgcctcc ccggtggaca
     3121 gatcaaatgg tacatgccaa tgatatttca ttttattatc gattgccggc tgataaccgc
     3181 gacctttttc cgtatggacg tgaagtacaa tcggatgatc aatatctttt acttcttcaa
     3241 ataaacggat caccgtttcc agatcgtttc cattatccac gtaacgataa tcaaatccca
     3301 ttgctttaaa taggttattg ctgcttttac cgttggtttg acgaagctca gctaagtttt
     3361 tatataaact gccatgattc tcatcaatag acatctcatt atcgtttact aatacgatta
     3421 gattggattg taaaacacca ccattgttca atccttcaaa agctaagcca ccacttaggg
     3481 agccgtcacc gataaatgct accacatttc ctgattcttt cttcagatct ctcgctttag
     3541 ctaaacctgt tgctaagctg atagatgtag atgtgtgccc cactgtaaaa tagtcatgtt
     3601 tactttcatg ttggttggaa tatccgctca cttcacggta atgtcctttt tcaaaaccat
     3661 tttttcgacc agttaaaagc ttatgaccat aagactgatg ggaaacatcc caaacgattt
     3721 tatcctttgg tgaatcaaat acataatgaa acgcaatcgt taattctact acacctagat
     3781 tcggaccaac gtgcccacct acatcgctgt ccttttgcaa gattttttgg cgcatctctt
     3841 ctgccaactg tttcatttcc tcaacagata attttttgac atctgcgggg ctattgattg
     3901 catccaatac atgtaatact gtactcataa tacattctct cctccgtact atcttctttt
     3961 aatgaaaata gatttgttac actttatcta ttctactact tagagttgac tctaagtcaa
     4021 tagcatttta aaaaaatttc agagaaagct tgttatataa gtagatcttc gcacgaatca
     4081 ggactcaaat aaaaaccttc tacagacaat taaaaagctt tgtagaaggt ctcctaatta
     4141 aggtctcatc aagaaatatg tttttttaat aggtgaagtt cctgagcaat atgatacaaa
     4201 tggatgtttg caccttttaa tgagattgga tagatctttg tcttttcagt ttctaatcca
     4261 ttttgatagc tagcagcatc gcacaaaacc actctttttt tcgcacatag acattcatca
     4321 ggcaattctt ctaatgaacg aaataaaata ggatttattt cagcgatgcc ttcttccgca
     4381 tacaaatgaa attgggttcg ataaaaaaat tgtttgactg aactttctga aacaacaaaa
     4441 atttctattt cctgtctccc cgcttgttca ctgttgatca aagaaagact tttgacgaat
     4501 tttcgaatga aattttcatt ccatcttagt tggatttgat ttttcttttt ccactcttcc
     4561 aatatttcta caatttttcc gtacagaagt tgttcttttt ctgatagaag atagatcctt
     4621 tccggtaaaa atacttgtcc atctgcccac aacgtacgga caaattgtaa aaaggattgc
     4681 ttgaaaataa aattttctgc atcgtcgatt tcagcgatac taataatctg taaaatcaat
     4741 tcattcagtg ataggtcatt gagaacaaaa tttttataaa caacttcgaa atcttttctt
     4801 aacagctcca tgttctcatt ggtataattt ctactgttga acaaagaaaa aatataatag
     4861 acatcttctt cttgtaagtc tagaattcct ttttgctctt ccaatccttg acagactaat
     4921 tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat
     4981 gcctttcttc cgattgcgat ctctattcct cgtatgataa aggacttttc atcttccgaa
     5041 tatcttcttt taaaaaaatg agtctctaca taatcaatca actcgttaca agcttcttct
     5101 acatacggat ttttattttc atagccttcc caattgacat aacgtactaa agctagcaag
     5161 atgttccgcg agtcaaattc tgggataatg atttctttat ttttcaaata atcattttct
     5221 ttaaaaaaat ccaatacgat ttttttgatt gtatatactt tgctcaaaga aataaattca
     5281 agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa aaactttaaa
     5341 aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc atgtttgaat
     5401 acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc tagacactcg
     5461 gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga aatagctgtt
     5521 tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac ttttctaaat
     5581 atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt tttcttatga
     5641 ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag aaaagccctt
     5701 tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca atttcagcct
     5761 cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa gaatggttat
     5821 tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat accagctgcg
     5881 ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg atttggcaca
     5941 gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg gacggaaaac
     6001 tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat tgtagcactt
     6061 tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc cagcacagca
     6121 gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa ataagcatcg
     6181 agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta agcaagagag
     6241 cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa tgtgatatta
     6301 ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc atctgcatct
     6361 ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg attcattgga
     6421 tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc ggttggttcc
     6481 gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa taacaatacg
     6541 ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt
     6601 tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac ttcagctaat
     6661 gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt tccaatcaat
     6721 tgttctaatt cttttacttc catttgaata accagctttc attttttatt tttctgcatg
     6781 ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga taagataaga
     6841 aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag caggtggaac
     6901 aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa cgattacttt
     6961 tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt taaaaaccag
     7021 cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa cagtcaatga
     7081 catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga cggctgccca
     7141 gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc
     7201 ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg cttttgaagg
     7261 agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa tcgcaagaat
     7321 tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta acgattgaat
     7381 cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg tcaatccgcc
     7441 aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg gataatcctt
     7501 tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca tatatgctaa
     7561 aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt
     7621 catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa
     7681 caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa tcaatgaagt
     7741 gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca ataggattgc
     7801 caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg ataatgcacc
     7861 agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat
     7921 tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga caccgccttt
     7981 ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga taaaccatag
     8041 caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga atgtgggata
     8101 ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga acgttaattt
     8161 ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct atttgaattt
     8221 gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt gttcttcttt
     8281 tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg tcagcacata
     8341 ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt accagctgct
     8401 tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg tacctgtgaa
     8461 tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt tctttttacg
     8521 aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca tggcactttt
     8581 tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg atcctgttag
     8641 tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc aagtaatcat
     8701 cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt catctgctcg
     8761 aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg taccgatagc
     8821 agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc tttgaccaag
     8881 tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg ctccaccagc
     8941 gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt ggcaaacagg
     9001 gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta aattcaatct
     9061 ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca gttggtaaag
     9121 aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg
     9181 tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc gtccaaaggc
     9241 caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga gaaaagattg
     9301 agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa aagctagacc
     9361 aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt
     9421 gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat aaaagaaggt
     9481 gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa agacagacaa
     9541 ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa
     9601 taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttt
//
LOCUS       DACDVT010000088         9338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDVT010000088 DACDVT010000000
VERSION     DACDVT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.090"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..282)
                     /locus_tag="IWL52_002263"
     CDS             complement(<1..282)
                     /locus_tag="IWL52_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9778789.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLYK"
     gene            complement(430..988)
                     /locus_tag="IWL52_002264"
                     /pseudo
     CDS             complement(430..988)
                     /locus_tag="IWL52_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1157..3268
                     /locus_tag="IWL52_002265"
     CDS             1157..3268
                     /locus_tag="IWL52_002265"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9778790.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3246..3896
                     /locus_tag="IWL52_002266"
     CDS             3246..3896
                     /locus_tag="IWL52_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9778791.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3883..4335
                     /locus_tag="IWL52_002267"
     CDS             3883..4335
                     /locus_tag="IWL52_002267"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9778792.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4352..4657
                     /locus_tag="IWL52_002268"
     CDS             4352..4657
                     /locus_tag="IWL52_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HAP9778793.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4669..5946
                     /locus_tag="IWL52_002269"
     CDS             4669..5946
                     /locus_tag="IWL52_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HAP9778794.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5965..7113
                     /gene="dgoD"
                     /locus_tag="IWL52_002270"
     CDS             5965..7113
                     /gene="dgoD"
                     /locus_tag="IWL52_002270"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP9778795.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7125..8027
                     /locus_tag="IWL52_002271"
     CDS             7125..8027
                     /locus_tag="IWL52_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HAP9778796.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8696..9082)
                     /locus_tag="IWL52_002272"
                     /pseudo
     CDS             complement(8696..9082)
                     /locus_tag="IWL52_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9142..>9338)
                     /locus_tag="IWL52_002273"
     CDS             complement(9142..>9338)
                     /locus_tag="IWL52_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9778797.1"
                     /translation="NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGITA"
BASE COUNT         3012 a         1447 c         1904 g         2975 t
ORIGIN      
        1 tttgtacagg tgtagataag ttggtacttc ttgatacgga agcaactgaa tccaactatc
       61 atgataagca tgacggatga cagaatcaaa cccacaacat tcgcaacgat gcgcttctcc
      121 agttaataag ccggtaaata cattggccat acggccatga acgcgctctt tactaatggc
      181 atcttttgaa aaggtaatat gggaatcttt gatgttcaaa atttgtttga tggaatgact
      241 tgtcttttca tccgtacatt tgatattctt agttatggac atttggttat cctcctatga
      301 ttgtttttgc cgatttaatt ataggacaac cagatgtctt tttgttataa aaaaatattt
      361 ggcgtcacta ctcagtttga gtaacaaaac caaatattat agaaccggct ttttcagttt
      421 tttcccttca caaacaacat cgatgatttt ctcggcatgg gtacgggtat cattcattaa
      481 ttctgtatcg gttggatatt tgatgttttg tggagcacaa gtggcatcca aaatcaatgt
      541 tccggaattg aaatcgtcat aagaatcgtt gtccttatcg tcttttttct tgtcgaagac
      601 gagtattttt tcattgattt ctatcaacgt tgtttcgtcc aagcgtttcc ggaaatagac
      661 cattgtagag catcgaaagg cttggcatct tgatagcccg gcaaaccgat gaagaactat
      721 aaatacgggt tttcttgaat ttgtaagacc gtttcttcgt cgctatagct atattccttt
      781 tgaatcagta gagctccgaa ggtcatgcga aagggttttg cgacgtttcc ttttttttac
      841 gaaaattttt agcgtagtct ttttcaagtt cagcccatgg aatgagctct gctttttgaa
      901 tccaacggtt agacggattc attttgagac ctatcggttg attgaaatct tcaaaagcca
      961 attgaacggt tgtttgtaat ttatacatga aaagcctcca aaattgagtg gtactccaca
     1021 ccattctgat ggtgcataag ataaaaaatt aaagaagtgc ctgtcatttg tctgatttaa
     1081 gccaaggttt gacaaatttt ggatattcca attaatactg taagcggtta tatttaactc
     1141 agggaggtag atgaatgtga atacaaaaga tttaaaactg ttagaaacca ttgtacgatc
     1201 acatgtgaca aactctcaac aattgaaatc attagtacat ttttcgacta ggcagataac
     1261 ttactctata aaaaaaatta atgatgaatt atcccttcat ggattgagtc tgataaagcg
     1321 aagtaactca ggggagtggt tatatgaagc caatgatctt caaaaacttt tgatatacat
     1381 gaatcaagca atggttttta aagaaggtgc gattcctgaa tatataaacg atgatttggc
     1441 ttatagtgag ataagaagat attcggaact gttatttatt ctagccaacc ccaaaaaagt
     1501 gacaacccaa gaaataatgg cgtttattgg tatcagtaaa aatacggcta gaaacgactt
     1561 ttctaaaata aaaaaagagt tgccaccaga ctacactttg atatttgatc cccaaaatgg
     1621 ctatatgatt gaatcaagca tggcaaagct tcatacattg ctcttagaag ttttcaatta
     1681 tttatttgaa aggcatgcaa cagttaaccg ttttgagtca ctcttttctg aagaaaggga
     1741 atttgttggt ttttttattc acacctacga acaaactttt aaattgaaat tttctgataa
     1801 ttcactaacc aaactttatt atttgatagg tttatcactt tctagtttta aaagaaggac
     1861 gaaaggacag attgatttta gttatgattt acaggaatta gatgagtttc aaatgattcg
     1921 aagctgcatt gatcaacatt atcaacatgc tgagatagct atagattatg aatggttgac
     1981 gcttctgttt ttatcattca atacaatttc aaatgagttt gaaattttag attcaagaat
     2041 ctatcaagta attatggaga tgattacctt atttcaaagt aaatcagcta ttcggattag
     2101 taataaagaa gagttgacca atcgattgat gaatcattta cgacctgcaa tctatcgaat
     2161 tcgctacgaa ttaccatttg aaatagatga tttagctgaa attgttaagg aaaaccatga
     2221 agttagtgcg gtacttaatt ttgttaaaga atccattgta cctttggaag catttttgcg
     2281 taagacgatt cctgaaactg aattatattt gatttctgtt tatttttgtt cggagctatt
     2341 aaattcttct attatcgaaa cacctgttag aaaaaaggca atcgtcgtat gtagcaatgg
     2401 attgattatg gccaatgtaa tggcgaaaac attgaaagaa atttttccag atattcagtt
     2461 tgttgctagt tcttcggtca gagagatgga taagtttgtc gaagatgttg atttgatttt
     2521 tagcaatcaa ccattgaaaa gtgaaattcc attatatatt atacaaccga ttatgaacga
     2581 agaagaaaaa aaccgcttgc ggaaccgtgt actcattgat ttttctacga ataatatcag
     2641 caaagagact aggaacctta ctgaaattat taagcgttac tctgctgttc aaaatgaaga
     2701 aagtctacaa actgatattg agagattttt aattaattat gcaaattcac taatatatct
     2761 agatcgtcaa agtaaacagg cacaaaagat atcttttgtg gaattgcttg tttctgaagg
     2821 gattattctt gtatctacga aacaacttac ttggcagaac gcgatggaat tgtcttgtca
     2881 gcctttatta aaggagcagg taattaatca gaactatcag actattttaa tgagtcaaat
     2941 ggcggatgaa gaggcttatt attttcttaa taatcgtatt gctattccac attcatcgcc
     3001 agaaaatggg gtaattaagg gcggttgctc gttacttata tcagttgatc caattatttt
     3061 tcctggaaat aggcctatcc attttatttc cccttttgca attggaaaga aggaaaatta
     3121 tttacttgcg attaaccaat tactacaact ttctaaggac gaggaatatc agcaagaaat
     3181 acttctttgc acgacggaag atgaagtgag aaaaaaaata aaaaattttt taaggaggaa
     3241 gcaatatgtc aataacgaat tttcctgaat taaccatcat tttaagagga tatagttttg
     3301 aagaggcgat gtttgtcatg gaagaattgg ctgcgatgaa cacttcggta ggaattgaag
     3361 taacaaccaa taatgatgac tttgcacgta tcatcacaga aggcaacaaa aaatttgggc
     3421 aacaattatt gattggtgca gggacagttt taacattttc acaagcagat caagcaattg
     3481 ctggtggtgc gaaattttta ttaggaccaa aaaaattttc aacaccaatt ttttcgttgg
     3541 ctaaagaacg tggggtggta actgtgccat cagcttttac gccatcggaa attcaagtta
     3601 tgtttgatga aggtgccgat attgtaaagg tttttccagc agtcactgta gggccaacct
     3661 actttaaaca actggccggt ccttttggta tacggccttt aatggcagtt ggaggagtga
     3721 cgattgataa tattgcggct tttaaaaaag caggagttgt gtactttggg gtaggatcaa
     3781 gcatgtttca aaaagctgat atacaaaacc gcaatgggca agctattcga aaatcaattc
     3841 aatcatattt agataaacta gctgaagcag gaagtgaaga agatgagtct cattaaatta
     3901 gaagaaaaaa atattttgtt aaatcaaagt ttttcaaacc aagaagaata ctttcaaaag
     3961 attacaaaga aactgatgac ggaaaacatg gtaaaggagg gatatctcga gtcaatcact
     4021 aagagagaaa caacttatcc aacggggcta tatattgggg aacgctcaat cgcaattcca
     4081 catacagatt atcagttatc aaatgttacc gctattttag tatccgttct taaagatagt
     4141 ttgcattttc agcgaatgga tgatcccgaa gaaatggttt cagtgaatgt tattattcac
     4201 cttttattcg atagtcccga taagcaaccg caattattga aggagttgat gagtaaagtt
     4261 accaatcagg atttcttaca agcaattatg gaagcaaaga gtaaaacaga agttttaact
     4321 gcatttaatc aataaattgg agggaaatac aatgagtaaa aaaacaatta tcgtcgcatg
     4381 tgcatcatca atgattacat ctacaatggc agctaataaa attcggaaaa tagcaaaaac
     4441 aaatggatta ccagaacctc gaattttgca actaaaattt tcagaagttg caggtgcttt
     4501 agaatcaaat caagttgact ttatcgttcc tacagggaaa ttacaagaaa atgtaaccaa
     4561 aggaattaaa gttatacctg gaacaccatt tgttactgga gtgaatgaag aaaaatctga
     4621 atctttggtg ttagaagcat tgaaagagct tgactaggag ggtgaaaaat gaatattgtc
     4681 attgatgcgt taaactggat ctcaagtttg ggtccgatga ttatgatgcc tatcattatc
     4741 ttgattttag gattattttt tagaattaag ctaaatacat tactaaaatc agcgttgact
     4801 gtggggattg gttttgcggg tgtaaatatt attattaatt ggtttatttc acaggtaggt
     4861 ccatctgttc agttaatggt caaaaattgg ggtattcaaa caagtattat ggatgtcggc
     4921 tggccagctc gtgcagcagc cacttggtct tttcctctag cagcagttat ggtatttatt
     4981 attttgggta ttaatgtgtt gatgctgatt tttaaaaaga ccaatacagt aatggtcgat
     5041 ttctggagtt ataatcacta tatttttgta gctgctttgg tttattatgc aacggataaa
     5101 aatattatat tggcggtaat cttcggggcg attgatgcaa ttattagttt taaactcgct
     5161 gattggacaa cgccgttagt agaggattac tttgaactag aaggggttaa tttcccgact
     5221 gcgaactcag ttggttgggc acccttggct tggctactta acaaattgtg ggaccgcatt
     5281 cctggattaa ataaggtaga tgctcgtcct gaaaaaattc aagagcggtt tggttttgtt
     5341 ggtgagccta tgtttatggg atttgtcatc ggcttggtta ttgggttatt agcaaaagaa
     5401 cctgttgata aggtattgat agtgggaatg agtacttctg caacaatggt tctaacacct
     5461 aaaatgatgc aaattttaat ggaaggatta ttaccctttg ctaattctgt taagaaattc
     5521 ttgaatgtaa aatttccagg aaacaatttt acaatgggtg tggatgctgc gttgacagtt
     5581 gctaattcaa gtgccattgc tacgggtatt atcatggttc cgattacctt agtgttggct
     5641 gctattttac caggtaacaa actcttacct atttcagata ttgcctacca agcaatgtgg
     5701 ctatctgcat ggccggtcgc cttttcaaaa ggaaatatta ttcgcggaat actttccacc
     5761 acgattataa cagcttgtgt gcttttaatt gctactagtt tagccggcat acatacacaa
     5821 ttagctttgg ctgggggatt ccaattacca gcaggaatga cacaaatatc taccgaagat
     5881 gcaggtacac acattattgg tttcatttta atgaagattg ctgagtttat ttcaaatatt
     5941 ttctaatatt caggagggca tacagtgaaa atttcaaaaa ttacagtcta taaagtaaaa
     6001 ccaagatgga tttttgtcaa aatatctaca gatgaaggta ttgaaggctg gggtgaaatg
     6061 atttctggaa ctaagactga aacagttgtg gcaggagctt atgagatagg aaatcgattc
     6121 ttagttggtc aaaacccatt tgcaattgaa cgtctgtttc aggaaatgca tcgttcattt
     6181 ttccgaggtg gtcctattaa tggaacgatt gtctctggac ttgaaatggc tttatgggat
     6241 attaaaggga aagccttaaa tgttccagtt tatgaattac taggtggagc agcccgtgat
     6301 aaaatcaaag tatattcttg gattggtgga gatcgtcctt ctgaagtagt agagcaggca
     6361 caagatcgag tagatcgtgg gtttaccgcc gtgaaaatga acgcgacaga agaattacac
     6421 tacattgatt cttatgaaaa agtagctgaa gtagtggaac gtgttgcttc aattagagaa
     6481 cgttttggag atacattgag cattggtgtc gattttcatg gtcgtgttca caaaccaatg
     6541 gctaaggttt tagcaaaagc actggaaccc tatcatccaa tgttcttaga agaagtggtt
     6601 ttgccggaga atgaagaaca tttcagtgaa gtagccaatg cagttgctgt accactagca
     6661 acgggtgaac gtttgtatac aagatggcaa tttaaatcaa ttcttcaaca gggagcgatt
     6721 gatatcattc aaccagatgt agctttatgt ggagggattt tagaaacaag aaaaattgct
     6781 gcaatggctg aagcttatga tatagcagtt gccccccatg caccatatgg accgattgcg
     6841 ttagcagcta cattacaagt tgattcttgt acacctaatg tgtttattca agaacaaagc
     6901 ttaggtattc attacaacaa agggtttgat ttgttagatt ttgtaaagaa taaagagata
     6961 ttccaataca aagatggctt cgtagatctt ccgacaaaac caggtttagg gttagaaatg
     7021 gacgaagatc gagtgaaaga aattgcacaa gaaggtttag tttggaccaa tcctaagtgg
     7081 aaaaattatg atggtacaat cgctgaatgg taggagatga gtgcatgatc aatacggtta
     7141 caggcccaat cgaagctgag tctttgggta caacatttat tcatgaacat ttatatgtca
     7201 ttcctaatga gttaagaaag tatgaagatt ataccttgga tgatatttat catagtattg
     7261 cggaagttat ggcttttaaa caagctggtg gaaaaactat cgttgatttg acaccaatta
     7321 attatggacg aaatccgtta gctttgaaaa tgatatctga agctaccgga gtgaatgtag
     7381 catttgttac gggattccat aaggaagagt ttcagccaaa atggttgaca acgctcaatg
     7441 atgatgagat ctttgagttg ctttattccg aaataactca cggagtcact tcacaaaacc
     7501 ttttgccagg agcaatgaaa gtaggtacga gtaaaaatga ggttacattg gctgaacaac
     7561 gaatactagc catagccggt caagtacaaa aaaaaacagg gattcccatt attacgcatt
     7621 gtgatggggg gacgatggga ctggagcaaa ttaattgttt aacaaaaaac ggtgcgaaag
     7681 caagtaatat ttgtttgagt catgtagatt taactaaaga ttttcactat ttagctcaaa
     7741 tctgtgaaac gggagcaagt atttgtatgg atcatattgg ccgagagttg gcgaatcatg
     7801 atgcagaccg cgtccaacta attaaacgac tggttgatgc gggttatgaa aatcagattt
     7861 gtctttcagg tgatatggga cgaaaaaaat atttgccaac ttatggagga acgcctgggc
     7921 ttacctatat tttaacaagt ttgaaagaag aactattaaa agtaatgagt gaaccaactt
     7981 ttaataaaat gattgagatg aatccaatta atatactgag taagtgaagt caattgaatc
     8041 attcagaaga actaatgaaa ttagttgctt tcgcagggtt aattcttctc tctgggtttg
     8101 gaaaagaggg cttagcttcg gattccctag ttaaactagg ggttttatca ggttattgta
     8161 ctgattataa gtacctgaat tattcatgaa atactttttc cgtcgtttta taggcagttg
     8221 ggtcaataaa aagatcagaa aaacgaacga gagactctca taacgtgcca ctaaataaaa
     8281 atcgtacgtt attttaaaaa atgggttgat tctaggcagt actttgtcgg tctattttta
     8341 gaaaaagagg ttctttgtca aacatacatg aattcgcaag ctcattcacc ctgaaggatg
     8401 aaaggcagaa actgtagtat actaatggtg gaaaggaaaa aaagccgcca gcaatttgga
     8461 aaagaacgtt atgtcgattg tcaaactggc ttgctttatt tctcgagaac cctgaaatat
     8521 agccaatgag catgtgtata aaaaattgat tttatatgaa ataaagcact cctttcctaa
     8581 aattattgga aaggagtgcg ttttttatgg aaattattta ggttctttgt caattgctat
     8641 tgttttgctc aaatcgtatg ttaggaaatg cttcggcatt tccttttttg cgttattaaa
     8701 tgatttcaca gatgttctgt tgaatcagga cacgcatccg gagatgacga aaagtccgaa
     8761 atccaaaagc aattcttttg attactttga tgaggttatt ctttccctcc aattttccgt
     8821 ttgaataggg atattttaag gagtttgtga ttgcttctgt gtgctttttc agataacaaa
     8881 ttgccttttt aaacccatca ttcaaggaat gtggcaatga ttcaatgaga ttgaaaaatt
     8941 cagtaaaatc ttttgcatca aaagcagcta ataactcttg atagacttca tagctttgtc
     9001 gtagttggtt atctatagag agtaggtaat caagcacctc cgactccgtc atgtatttcc
     9061 cttggaagag gggaaattgc ttaaaagggt tctgttgcga agtttgaaaa tcaaacgcgc
     9121 cctctctgaa ctccaaatat cttaggctgt tattcccatt aataccttga tttcagtaga
     9181 caccgaaaag ccgaagagcg ttccatttct tcggttcttt ttatatattc ctcgaatggt
     9241 ctccatgccc ttaatcgtgg aagaggctgt acggagactt tgataaaatt tatttcgtcg
     9301 tttaataggt cgatggtctt gttctattaa attgttaa
//
LOCUS       DACDVT010000089         9312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDVT010000089 DACDVT010000000
VERSION     DACDVT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.091"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            288..821
                     /locus_tag="IWL52_002274"
     CDS             288..821
                     /locus_tag="IWL52_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778798.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(947..1624)
                     /gene="nth"
                     /locus_tag="IWL52_002275"
     CDS             complement(947..1624)
                     /gene="nth"
                     /locus_tag="IWL52_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9778799.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1631..2329)
                     /locus_tag="IWL52_002276"
     CDS             complement(1631..2329)
                     /locus_tag="IWL52_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9778800.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2597..3781
                     /locus_tag="IWL52_002277"
     CDS             2597..3781
                     /locus_tag="IWL52_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9778801.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3787..4230)
                     /locus_tag="IWL52_002278"
     CDS             complement(3787..4230)
                     /locus_tag="IWL52_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9778802.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4456..4743)
                     /locus_tag="IWL52_002279"
     CDS             complement(4456..4743)
                     /locus_tag="IWL52_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778803.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4914..6266)
                     /locus_tag="IWL52_002280"
     CDS             complement(4914..6266)
                     /locus_tag="IWL52_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9778804.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6539..6985)
                     /locus_tag="IWL52_002281"
     CDS             complement(6539..6985)
                     /locus_tag="IWL52_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9778805.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(7221..8147)
                     /gene="cysK"
                     /locus_tag="IWL52_002282"
     CDS             complement(7221..8147)
                     /gene="cysK"
                     /locus_tag="IWL52_002282"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9778806.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(8287..9174)
                     /locus_tag="IWL52_002283"
     CDS             complement(8287..9174)
                     /locus_tag="IWL52_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9778807.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2576 a         1874 c         1506 g         3356 t
ORIGIN      
        1 aaccacgtct acaagcgtgg tttctttttt gtgtctatgt aaaaaacagc tacaaataaa
       61 aatcatgtac caagctgtat tacatgattt tttataagac tatccagcat atacatgtgt
      121 gatctaacat ttagcttaaa aattagaaaa tccatttttc ttttatcatt acatatactt
      181 tatccattta cttgtttata attaaatcgg ctaaatacac actagtcttt tatttagtgc
      241 cacttcttaa taatcccatt gttattatca aataaaaaat acaatatatg gttttgaata
      301 attttttgaa agaaggcttc attttgagtt atctgatagt atgcttaatt atcataattt
      361 gtctgcacca tcttttttta tcgaaaacaa tacctcgtta ttccaagaat aaaaaagctg
      421 aaaaattttg tttattatta aataaattta ctttaattgc tcctatcctt gcatttatta
      481 ttttttctgt acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg
      541 caatgattct cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc
      601 tgaaaccgcc caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac
      661 ttatcatttt tattacacct ttagatagat acgtcactta tttatataat cctttggaat
      721 actggactta ttttataggt atattggaag gcattatttt ctacatcggg tattttccca
      781 ataaaaataa caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta
      841 ctgagtatgt acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata
      901 ataacaagat aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt
      961 tcttttttag cgccttttct tttagacgca tccgattttt cccatcttga catattgata
     1021 gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa
     1081 tcagtgtatg atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt
     1141 ctacttccat gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca
     1201 cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg
     1261 tctttctgcc tacaccagga agagacatca attcttctct tgacgttggc acttgcccat
     1321 cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca
     1381 aaccaatcgt ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg
     1441 gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct
     1501 gggcacttaa gatcactgca atcaataact caaatggatt attgtgcttg agttctccat
     1561 gtgcttcggg aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta
     1621 acatattccg ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt
     1681 gagctgactg ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt
     1741 ccacttgttg ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt
     1801 aattaaaact ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg
     1861 gctgataatg gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg
     1921 ggcgtccaaa ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt
     1981 tttcttcgta ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt
     2041 aaagattata tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc
     2101 catttgttac gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca
     2161 tagcaatcat ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta
     2221 gaacaaataa aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat
     2281 tagatatgac tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc
     2341 cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt
     2401 tacttcactg atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt
     2461 ttgatgcgta tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga
     2521 atgttgacaa ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc
     2581 ataaaggagt ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt
     2641 ctctgtctta ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac
     2701 gctgccttat tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt
     2761 ttatgctcta gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg
     2821 gcgtcatccg atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg
     2881 tttaggaaat tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg
     2941 agaaatctcc accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa
     3001 agtttttgga tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg
     3061 gggtctatta gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt
     3121 cttaaatgcc gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc
     3181 agtagattta agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat
     3241 ttcatccgtt attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct
     3301 tcaatctatc ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg
     3361 tttaagcttc tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg
     3421 attgctcaaa aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct
     3481 cgcctatcta ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg
     3541 gattatctgt tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc
     3601 caattctgtc tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc
     3661 tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct
     3721 tccgacggta ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata
     3781 gtagcttcat ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt
     3841 gtcaaattct tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg
     3901 atggagttgc tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat
     3961 ttctttttta ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt
     4021 tgttagctta ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt
     4081 aggatcaggg attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc
     4141 aaattctttt tccatttctg gtttattacg atgaataatc tcattagcta aatccagata
     4201 ttcttctatt agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt
     4261 gtttttttgt ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa
     4321 ccaagaaaaa aaggggttga caaagcttgt cataacccct ttttctgatt aaaagatagt
     4381 aaagagacag tcatcggtaa ttgtgatgat tttttgtcat cacttaagga tgagcggcct
     4441 tttcaaaaac tgtctttaaa gatcatcatc agtttctagc acatatggct gaccttcata
     4501 tgtgtactct gtattcaatg tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg
     4561 aaaacgatga gaaacattat ctttggcgtc atttgctggc atcgtgtaat attcctcttg
     4621 cgtttcattc aactgagaga gtacttgatc tgccaactca tcaatattga tcgttaattt
     4681 cttcgaagca cttttataat cttctaaaaa tgcatcttgt tggacaaaat attctttatt
     4741 catcttcttt ctcacctttc ttttcaaaat caaataaagc tgcttgttct tttatgtaac
     4801 agctttatct ttgctgctta ttgtgacaaa aaattcgaag aaagacaact aattgtactt
     4861 ttcacaaaaa aagacagagg gtcgtgacaa aattgtgtca caaccctttt tctttattgt
     4921 gcagtttcga tttcgcctag cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg
     4981 tcaaaatgtg gttggaagaa gaaatctgaa agtgcgagat cttctacagt cattttattt
     5041 tgaacagcta aagacatggt atttgctgat tgtgtaatgt cgtatttgga catgaactga
     5101 gcacctacga tacgattcgt tcctttttca tagactagct ccatcattac tttttcagta
     5161 gttggcatga actctggacg ataattatct tcaaatacag tcgcttggac ttctagatcg
     5221 ttcaaagttg cgctttcttt ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa
     5281 agtcctgaag ttccttgagt accacgatac gccattttat gatctgtcaa gttatttccc
     5341 acaagcaaac cttgacgaac ggcatttgtt gccaaaggaa tatagttttg cgtgccactt
     5401 ggattgtagt tgactactgc agagtctcca gctgcaaaaa tgtctggatt acttgtctgc
     5461 atgtattcat tcactttgat tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt
     5521 tttgtatttg gacggaagcc cacacataaa atgaccatat ctgtatcaaa tgtatctgtt
     5581 ggtgtagcta cttgtttcac ttttccttct tcatctgtga taaattcagt aacattttcg
     5641 cctagcgcaa gagtaacacc gcggtctgtc aattcttttt ctaagatatc tgtgaatggt
     5701 ttatctagat atttattcaa gatgcgatct aatccatcaa tcaatgtcac ttgttttcca
     5761 gattcgacaa aagcttctac taattcaata ccgatatatc caccaccaac aattactaca
     5821 cgttttgctt ctttcgcttg tgcaataatt tcatttgctt gattgtagtt tttgcatagg
     5881 agtacatttt tcgcatcgat tccactaatc ggtgggataa taggccatga accagttgtc
     5941 atcactaatt tatcataaga aaccgtttct gtttctcctg ttttcagatc ttttgccgtt
     6001 actgttttat tttctgtatc gacgtctgtt acatcgtgtt ccattttaac tgttgctcct
     6061 aatgaagata attcttcagg atttgagtag aacaagcctg ctgggtcctt tactactccg
     6121 ccgacataaa gtgcgatacc gcaagataag aaagaaatat tgtcatttct ttcatatact
     6181 gtcacttctg cttctggatg attttttaaa atacttttta cagctgcagt acctgcatga
     6241 gtacagccaa ttactactac tttcatttat ggtccctccg attaacattt tgtccttcga
     6301 caaaactact ataacagaaa taaaacggat acaacagttt aatgcttgtg aattcacaaa
     6361 ggtttgtttg tagtttcatt ccttttcaag cataagaacc caatatctac aagattctta
     6421 ttttaataat attcactttt atatctaaga ttgtgaataa aagacaaaaa aataaaaaag
     6481 ctagcgtttt ttttacacag ctagcttttt ttaggtataa cacttgtaga aactgttttt
     6541 acatttgcgc cttttttgct gactctttac aatccggaca gagtccatac aattccaacc
     6601 gatgttcatt aatctcaaaa tcagtcagtc tgcttgcagc catttctata tcttcaagcc
     6661 ctgggtaatg aaaatcaaca atcttaccgc agttttcaca aattacatga taatgacgct
     6721 ttgaactgaa atcaaatcgg ctagaagcat cgccgtactt catttcttga acaaatccaa
     6781 tcgaagtaaa taagcgaagg ttattgtaaa cagtcgcaac actcatattt ggaaaccgat
     6841 gttcaagtgc tttataaatc tcatcagccg ttggatggga atgatgttca atcaaatatt
     6901 ctaaaaccgc gtaccgttga ggtgtgattc gaatgttctc tttttttaac ttttcgataa
     6961 cctcttcgac taacttattt tccatgtaaa caccccacgt tgttattctc gtcttatcat
     7021 actattttta cattttaaaa tccacttaac atgaaaagaa aattcaattt tattgtattt
     7081 taatgaaaaa agacgtaaaa cagtcgcgtt cactattcta cgtctctatt caaataattt
     7141 ttctcatcat aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat
     7201 ctggagaaaa accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca
     7261 ccattgtcgg gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt
     7321 agcgcggcag caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca
     7381 acttctctag ctgtttccat cgcttcgtct ccactaatag aaaggacttt atcatacaca
     7441 gaagtatcca gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc
     7501 gcttctttcc cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc
     7561 ggatacacac gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct
     7621 gcaacaaacg cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc
     7681 ttctcatgca caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt
     7741 tcttttgcca aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc
     7801 aaaatcaact ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca
     7861 ggcatcacga tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct
     7921 gtatttccag aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct
     7981 gctttttcta tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac
     8041 tcaagcttta caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt
     8101 ggcgttttgc caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc
     8161 ttgtctttgc tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt
     8221 ctttgactag caaaaaaaag acaaagatat accatatatg gtaatttctt tgccttttta
     8281 tctaaatcaa acgtttagtt cttgaccaac taataaagaa taactggaca tagacatgcc
     8341 atttttcgcc attagatcat caacagacat gccaaatttt tgtgcaatat cccatagcgt
     8401 atcacctgac acgactgtat atgtctgacc attatttgta tttacatctg ttgttgaaga
     8461 tgatgacgtt gaagtcgtcg tagtagtcgt tgttgttgta cttgtggttg ttgtagccga
     8521 acctgttgat ggcgtatcaa aacgggttaa attataagta ttaatcaatg agttcaattt
     8581 ggccccataa tttggatcgg tagcataacg acctgtcaag tacgctgttg catcataaaa
     8641 acttgttgta ttgcttttcc acacgccagc ataatgataa tcgcccgtgg aaaaactggt
     8701 tgaacgaaga acattcgcat ggtcttggaa ggattcccaa taagaagaat attttcggaa
     8761 tggctcattc atcgttaccc attgtccgtt tacatattct ttggttgcca tataaacgac
     8821 ctgtccgtta tagcttcctt tcacaccgaa tagattataa tttggcgcac tagctaatcc
     8881 ggaagtgcca tatgcacttt ccaacaacgc ttgagcgatc atgactgaag catacaagtc
     8941 attacttgca gcaacttctg acgcagacca gccgatttga tcgataaacg cttgaggatc
     9001 agcgactgtt tgagatcctt gttcatctgc atgaacatgt tgcggtaccg ctgtaaccat
     9061 catcggccca actaaaagac tcgttccaat taaaggagca atcgcttttt tattgatcac
     9121 catttttctt ttaggtctat ggtgccgggc actgcgggtc ttcagctctt gcattcaaat
     9181 tcctcctcaa aataaaaaaa cttaataaaa agttctcttc ttaaaacaaa cttaattaat
     9241 taaattatac ttttaattcc gccgtttgta taattaagcg agacaaataa aaaagccatt
     9301 tatgttacac tc
//
LOCUS       DACDVT010000090         9091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDVT010000090 DACDVT010000000
VERSION     DACDVT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.092"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(41..1885)
                     /locus_tag="IWL52_002284"
     CDS             complement(41..1885)
                     /locus_tag="IWL52_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778808.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2097..2615
                     /locus_tag="IWL52_002285"
     CDS             2097..2615
                     /locus_tag="IWL52_002285"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9778809.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2743..3426
                     /locus_tag="IWL52_002286"
     CDS             2743..3426
                     /locus_tag="IWL52_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9778810.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3735..3808
                     /locus_tag="IWL52_002287"
     tRNA            3735..3808
                     /locus_tag="IWL52_002287"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3769..3771,aa:Arg,seq:tct)
     gene            3967..4326
                     /locus_tag="IWL52_002288"
     CDS             3967..4326
                     /locus_tag="IWL52_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9778811.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4449..6080
                     /locus_tag="IWL52_002289"
     CDS             4449..6080
                     /locus_tag="IWL52_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9778812.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGELVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6142..6783)
                     /locus_tag="IWL52_002290"
     CDS             complement(6142..6783)
                     /locus_tag="IWL52_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9778813.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7065..7373
                     /gene="rplU"
                     /locus_tag="IWL52_002291"
     CDS             7065..7373
                     /gene="rplU"
                     /locus_tag="IWL52_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9778814.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7400..7744
                     /locus_tag="IWL52_002292"
     CDS             7400..7744
                     /locus_tag="IWL52_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9778815.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7757..8050
                     /gene="rpmA"
                     /locus_tag="IWL52_002293"
     CDS             7757..8050
                     /gene="rpmA"
                     /locus_tag="IWL52_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9778816.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(8143..>9091)
                     /locus_tag="IWL52_002294"
     CDS             complement(8143..>9091)
                     /locus_tag="IWL52_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9778817.1"
                     /translation="TFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYPKFGNIKLSK
                     INTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLNLLIPKPQIK
                     TEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEILALTWDNIN
                     FKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLEQRKYLFQLG
                     FTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASMLYEAGADHK
                     EVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
BASE COUNT         2840 a         1479 c         1810 g         2962 t
ORIGIN      
        1 aaaaaatagc ggaatccaca tatatggatt ccgctatttt ttatacagga ttcgggtaat
       61 ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc gttagtgttc
      121 ctgtgtaacg atccgctacg tctttcatca tcattaagtc aaaacgttcc tgatcatcta
      181 attcttctgt gatgcctcga ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac
      241 aaatgacgaa atgttcctct tgttcgtgta tcgacaaata aatttttcga tacagtggat
      301 tttcgatggc atgagtttct ctaaaggaat gctctaaagc gtggatcaaa agttgagcca
      361 gatcgtcatc gttgatagga agttcttcgg gaagatctgc ttggaaggtt atctgaatat
      421 tccgtttggc agctaacttc tggtaacgcg ctaagataag atttgtcacc tggtggttgc
      481 aaaaagaaca cagcatttcg aaattttttg cttcttctaa tatcttttcc acatagtttt
      541 tagcttttgt ttggtccgta tgcattaaat cttgcagatt ctccatctct tggacaaatt
      601 ccaggcgaag tgtatttact tcttggatat gctgtcgaag ctgctcataa tgttgctcca
      661 acaaatcatt tttgattttt aagaaatcta cttgtctccg atctttgtcg gtatttaaga
      721 tgtgatcgat tagattgtag ctgataataa cgattagaat gataatatat aaaatcgtag
      781 accaattgat cgttgtttga tagataccta atgccgtttg gatgtaaatc aagcaatgga
      841 taatggctac caagacgatc caaggtgtac aagctacata aaaacggtta ttcttatatg
      901 cttcgccaat gcatgcaagt gaagtgacta aagtgcttat aacactagca atattagcaa
      961 tcggcattaa ttcaggaagt tctgcaggag taaaagttgc ataaagcaga acgccgataa
     1021 aaatcaaaag ttggaacgta caaaaaattc cgtaccattt tttatagtaa aacattcggc
     1081 tccaatagta gcagatgatg aaagctgacg ttaagataat aaataaatga gataaaaaag
     1141 aatgaacgaa tggttcaaaa agaataccac ttaaaatatc ttctgataca gcttcaaatc
     1201 ctaatgccaa agcaaagcaa cttaagagaa taagtgagta ttcaagacgt ttttgtttat
     1261 acatcaagta gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg
     1321 tcaatacttg cggcactgat tgtgaataaa taaaactaat caatggtcca gttgttccta
     1381 gataaacttt cggtggcagt ccagcatagt tctgataagg agatgagact gtaagggtca
     1441 atttttttcc gatataattt gctggtaaat ctatcacagc caatgagagt ccgggattgc
     1501 tttcttcctg ataatctttc cgttggtata aaatcttttc gcctagatat acagtaatcc
     1561 attgatggtt tcctttgatt agtaaatttg cttcgtccaa cggtttttca agtgtcgttt
     1621 ccataatgaa tgtttcgtct ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa
     1681 acttaaaatc aggatcatct tttgtataga aaacccacga atcttttatt tctttcattt
     1741 gattgctgtc cgcaggagaa aaaataaaat aaaagaaata agcaattcct gttaaaaata
     1801 acaatgataa tataatgatt atgctccact ttatacgtaa ataaaaagat tcttttttat
     1861 tatatatgtt attaaaaaaa gacataaagg tattcctttc taaaacttgt aatgttaggg
     1921 taaacgtttt ttaaactaac gtcaattatt tataataaaa aatgagataa agatgaggaa
     1981 atagaattag aaacatataa ttattaaagg gaaacatttt tacagctata atttgaaaaa
     2041 aatttatcgt tattatatga ttaagaagga ttgaataaag aaagaagttg aaaacgatac
     2101 ctgattacaa aaaaataact tttataagtt ctataacgtt gattatattt ttaattctta
     2161 ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt attgaggaca
     2221 aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg aaaaatacaa
     2281 atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa aaaatctcga
     2341 cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa gctgatacaa
     2401 agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt cccatctttc
     2461 agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt caggggttat
     2521 tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt gtagagttga
     2581 aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc
     2641 cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc agcttgtaga
     2701 tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg
     2761 catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca aaaaaaatta
     2821 ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc agttattccc
     2881 catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga acgaatcttg
     2941 gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat cggagaaaac
     3001 gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat
     3061 gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc agatatcttg
     3121 ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc aaaatatatt
     3181 gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat ggtagatgac
     3241 atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg caaacgagct
     3301 atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga tttgacatcg
     3361 acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag
     3421 ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc tcaattcgaa
     3481 cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa tctaaataaa
     3541 caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta gaacagctga
     3601 aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa ttcattattt
     3661 gctaattaaa taactatttc gtattgacag aagcaattta attatctata attattgatg
     3721 ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc taagcggacg
     3781 gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga ggtttctaac
     3841 ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata aaataaaaaa
     3901 acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag aaaagaggaa
     3961 gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga atttttttat
     4021 atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt ttttaaaatg
     4081 agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca aggagtgtac
     4141 aatggactat taggagtggg actactttat ggagcttatc tttcttccgc atctaaagaa
     4201 atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag tttgactagc
     4261 gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct ttctattttc
     4321 ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa aatattactt
     4381 tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat gaaaaaggag
     4441 ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca ggttatgatc
     4501 tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta gcattgcctt
     4561 tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg attacggcta
     4621 tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata tcaggtccga
     4681 caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat
     4741 tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc cgattaggaa
     4801 cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt attgctatca
     4861 ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt acaaacgtta
     4921 ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc tctttgatca
     4981 tgggagagct agtcatttta gggatgttta tctatccaaa aaaatgggcg aaacgaatgc
     5041 ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc catttaccag
     5101 tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg aatatgggag
     5161 attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt gctttattag
     5221 gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat aagcccttag
     5281 atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg
     5341 ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct ggtgcacaaa
     5401 ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt gtttttgcgc
     5461 cagtgatgtc acagattcca atgccagcac ttgcaggtgt tttgattgtg acggcttggc
     5521 gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg tcagcagttc
     5581 tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt gctatcgtga
     5641 tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc acgatttcat
     5701 tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg aataactgga
     5761 cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc aaaaataaat
     5821 tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta ccaagtattg
     5881 atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa agaggacaaa
     5941 cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac ttgtgggaac
     6001 atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta catgcgaaat
     6061 atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa actgaagatt
     6121 ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta cttgccagtc
     6181 ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat gaaaaagtgg
     6241 aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa
     6301 gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt tgccaagtaa
     6361 acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct tcatcagttc
     6421 gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc gatttggata
     6481 aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat acgggcggct
     6541 ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag aaagtgcctg
     6601 catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct ctgttttccc
     6661 tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag cgataaatcc
     6721 ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt ttgggtattc
     6781 catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat gcgactattt
     6841 tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt tgttttactt
     6901 tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta ctgttgtaat
     6961 tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata tgctgtcact
     7021 tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac gcaattatta
     7081 aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt gaaaaacttg
     7141 acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc ggagaatcta
     7201 caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt gaaaaacacg
     7261 gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca caccgtaaac
     7321 aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt
     7381 tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg caggtcagat
     7441 cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg atatcgtttg
     7501 tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat tagccggttt
     7561 tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg aagtcacttc
     7621 aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc ttttattagg
     7681 attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa ttaatgataa
     7741 ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc acaaaaaagg
     7801 tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc
     7861 tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta caaaaatcta
     7921 tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag ttgatggcgt
     7981 agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc cagtagctca
     8041 agaagcttaa taaataaact gctctatctc ttgtgtagca agggatggag cttttttgta
     8101 aattatggga ttttttttga ccatactttt gaccatactt tcttagaagt taatataatt
     8161 tgttagtttt tgtgttgttt cttctttttt gctgttagta atgtgtgtat atgtgtccat
     8221 agtagttttt atatttgcgt ggcccattct ttcttggact tctttgtgat ctgcgcctgc
     8281 ctcatatagc attgatgcat gtgtatgtct aaaaccatgc agaccgatat aaggtaagcc
     8341 ggcacgctca cgatatatat tgtatctttc tgccactgac tgattaattg tgaaattatt
     8401 ttcgtcattg gtaaacaaat aattagcttt agtaaatcct aattgaaata agtactttct
     8461 ttgttcgagc ttccaaaatt ttagttgctt tatagttttt tcatctaaat aaattattcg
     8521 atttgatttt ttggttttag gagtttcaga tatataatat ttatctgaac gagctacagt
     8581 ttttttaatg gccatttcat ttgttttaaa attaatattg tcccaagtga gtgctaatat
     8641 ttcgccgatt ctacatccgc tgaatgctaa tagtctaaag agcgtatagt ctctgttttt
     8701 aaaatatgga ttctgttctt gagatacttc atttagaaat aattccaact gttcttttgt
     8761 atataatttt aatttctttt ctgttttaat ttgtggcttt ggaataagta aatttaatgt
     8821 tggattatca gatgttaaac caatatttat agcatactta aaaactttat tcatatagtt
     8881 tatgaaaaga gggtactgct ttgaagttcc tttttcactc caatcattta ctaccttttg
     8941 acaatatgcc gtattaattt tagaaagctt aatatttcca aatttgggat atatatgttt
     9001 attaaagatt attttagtcc ttgaatatga actttctttt acagtttttt tgtagctttc
     9061 tagccataat tctgctgctt ctttgaaagt a
//
LOCUS       DACDVT010000091         8934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDVT010000091 DACDVT010000000
VERSION     DACDVT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.093"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            21..320
                     /locus_tag="IWL52_002295"
     CDS             21..320
                     /locus_tag="IWL52_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778818.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(417..851)
                     /locus_tag="IWL52_002296"
     CDS             complement(417..851)
                     /locus_tag="IWL52_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9778819.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1083..1793)
                     /locus_tag="IWL52_002297"
     CDS             complement(1083..1793)
                     /locus_tag="IWL52_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9778820.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1783..2643)
                     /locus_tag="IWL52_002298"
     CDS             complement(1783..2643)
                     /locus_tag="IWL52_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9778821.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2648..3286)
                     /locus_tag="IWL52_002299"
     CDS             complement(2648..3286)
                     /locus_tag="IWL52_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9778822.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3301..3600)
                     /locus_tag="IWL52_002300"
     CDS             complement(3301..3600)
                     /locus_tag="IWL52_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9778823.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3643..5127)
                     /locus_tag="IWL52_002301"
     CDS             complement(3643..5127)
                     /locus_tag="IWL52_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9778824.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5147..5608)
                     /locus_tag="IWL52_002302"
     CDS             complement(5147..5608)
                     /locus_tag="IWL52_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9778825.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5626..6693)
                     /gene="ulaG"
                     /locus_tag="IWL52_002303"
     CDS             complement(5626..6693)
                     /gene="ulaG"
                     /locus_tag="IWL52_002303"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9778826.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6911..7681
                     /locus_tag="IWL52_002304"
     CDS             6911..7681
                     /locus_tag="IWL52_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9778827.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7954..8781)
                     /locus_tag="IWL52_002305"
     CDS             complement(7954..8781)
                     /locus_tag="IWL52_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778828.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2722 a         1874 c         1396 g         2942 t
ORIGIN      
        1 gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa ctacttaagc
       61 aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa gtgacagagt
      121 atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca aaaggaatat
      181 acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct ttaaaaaaaa
      241 atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt gataaagagg
      301 tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac taagtctatc
      361 ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat tatatgttat
      421 ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga aataccaccc
      481 atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt ctcagtatca
      541 tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa agcttcattc
      601 aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat atcttgaata
      661 aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt cccttcccca
      721 ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc agtgtctttt
      781 gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat cataagggaa
      841 tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat acagttcttt
      901 gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc cttaaaatct
      961 ctctttcctt ttagaatgca tcctttttga taaataaaaa ggttgtgata aattttcttc
     1021 acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt accctagcat
     1081 ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt atctagtaaa
     1141 tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc catcagacaa
     1201 atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc ccatgtgaac
     1261 ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt tctttcctta
     1321 aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac ttcttctttc
     1381 gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt cgtgccataa
     1441 gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg agtatgagta
     1501 atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag atctgaagat
     1561 ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac catttgatct
     1621 gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc tagctcacgg
     1681 tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg caatgccaga
     1741 tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga catcatagcc
     1801 agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc ttcgaaagtc
     1861 ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta gacgtttcaa
     1921 atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat cacgaaactg
     1981 gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag tgatataatc
     2041 gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca aattgcctag
     2101 gtctggatat acttgtagaa aaggggaatg aatctgttct ttgatttcta aaaattttga
     2161 aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc catatttaga
     2221 agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat tcattgattt
     2281 ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta gattatgcgc
     2341 caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg gatcagcaga
     2401 accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta caccggagct
     2461 gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc gtgctaaccg
     2521 ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt ttacaaggga
     2581 aaaacgttct ttccatgaaa tatctttggg taatgctttt tcataaatcc caatttgagc
     2641 catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg gccttcatcg
     2701 gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg atcccttcaa
     2761 ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag cctagttcaa
     2821 tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat aacagcgcat
     2881 cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc atggcttgtt
     2941 caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt tgtgctgcct
     3001 ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct gcttttgcaa
     3061 cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact agttttttat
     3121 ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag caaagaattg
     3181 tgcccacttc aataatatct actacatcac caactttcat aacatctcct aatgcatgtt
     3241 ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta gttccttcct
     3301 ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt ctttggctga
     3361 catcacattt ttgataccga taactgtaac ttcttttttc tctgcctgag taaacatatt
     3421 caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt ttccctctga
     3481 aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg ctttttccac
     3541 acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta aaattctcat
     3601 cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc tctgcttttc
     3661 tgcatatgtg tcatagtctt ctacaatcaa aaaataaccc tcaggatttt tccgatattg
     3721 taactgcgga atagccaata aaccaatgac aactacggca attccagcat aacctaggac
     3781 tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc cgatataacc
     3841 accatatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta cttgtatgat
     3901 tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat tggcataaac
     3961 tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga tcgttggtga
     4021 tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa acaagaaccc
     4081 aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa ttcctggaac
     4141 agctcctggt aaaattgtgt ttgagattcc ttggaaagat tgagttagtt catcgacgaa
     4201 tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag aagtcgtcat
     4261 aatgtagaat aagaaactct gtccttcttg catgaattgc gcttgaatca aataatcttt
     4321 gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag tagcaaccat
     4381 attctcattg aaaatcgaca agaatcctgg taatttgata tcttcgattt ttttgtcttg
     4441 cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta attttgcaaa
     4501 tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt ctgtcaattc
     4561 ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta aaatcagtcc
     4621 cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta aaatccaaaa
     4681 agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac gcagtttcgt
     4741 atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta ataaaatcat
     4801 cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag cattttgtcc
     4861 aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc caaccaaaat
     4921 cggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt aaccgaccgt
     4981 tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa tatagcctaa
     5041 taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg caaaatacgt
     5101 ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa ttatattctg
     5161 ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca accaatccat
     5221 ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct ggatcttttg
     5281 ctgcaagtgt aaaaaacaaa ctagccgttt tctcctcatc ctccccttca ctttcaaaac
     5341 ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca tcactttttt
     5401 ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg tatttttcta
     5461 cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg atcaatacaa
     5521 gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg taatgaatca
     5581 attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata aaaacgacgg
     5641 aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa aattaaactc
     5701 aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga tataaggttt
     5761 aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct cttttgaatc
     5821 agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg cattcaatga
     5881 ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc gtgggttttc
     5941 accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg caaataaatt
     6001 ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga ataaatagtt
     6061 tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt ctggatcttc
     6121 acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga tgtctttgat
     6181 tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc cccatttttg
     6241 ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat tttgatgaac
     6301 tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga ttaaagcgtc
     6361 tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat taggctgcat
     6421 attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc cattcccatg
     6481 actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt catgactttt
     6541 cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat ccactacttc
     6601 tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta ctaaccactt
     6661 ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat atcgttgttc
     6721 caacttacaa gtaaatatta atgtgtatca gcatccatgg aaaccctttt atatgaccgt
     6781 ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa taaagacaga
     6841 tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg tccctcagga
     6901 ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag aaattttgga
     6961 actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc ttgatgtatc
     7021 tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga tcatacgcaa
     7081 ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat ctggtcctga
     7141 aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt atgatacgct
     7201 gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat ctaaccattt
     7261 gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata attgtagtta
     7321 tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg acatcgcatt
     7381 aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg gagtcgatgt
     7441 agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa tgatggtcaa
     7501 caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac tgacatcaaa
     7561 gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat tttgttctga
     7621 aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag attcatttta
     7681 agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca aatttatcga
     7741 taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgtg tccatccaat
     7801 cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta aataggaatc
     7861 aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc gaataaacgg
     7921 tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt caatcacctt
     7981 taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat atctttctaa
     8041 ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca agtattccaa
     8101 tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata attggattgc
     8161 tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata caatgcagac
     8221 catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca cataaacttt
     8281 cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag ctgcttgtaa
     8341 gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa ataatgctgc
     8401 atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa tttgatttaa
     8461 aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat aagattcttg
     8521 gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga ttttgtcttc
     8581 ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa gcgcacttaa
     8641 gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg cttgttcaca
     8701 aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt cttttagtaa
     8761 tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt tctcctttat
     8821 atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat aaaaatttta
     8881 attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt tctt
//
LOCUS       DACDVT010000092         8425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDVT010000092 DACDVT010000000
VERSION     DACDVT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.094"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            387..812
                     /gene="lacA"
                     /locus_tag="IWL52_002306"
     CDS             387..812
                     /gene="lacA"
                     /locus_tag="IWL52_002306"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9778829.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            829..1344
                     /gene="lacB"
                     /locus_tag="IWL52_002307"
     CDS             829..1344
                     /gene="lacB"
                     /locus_tag="IWL52_002307"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9778830.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1355..2287
                     /gene="lacC"
                     /locus_tag="IWL52_002308"
     CDS             1355..2287
                     /gene="lacC"
                     /locus_tag="IWL52_002308"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9778831.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2290..3270
                     /gene="lacD"
                     /locus_tag="IWL52_002309"
     CDS             2290..3270
                     /gene="lacD"
                     /locus_tag="IWL52_002309"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9778832.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3298..3615
                     /locus_tag="IWL52_002310"
     CDS             3298..3615
                     /locus_tag="IWL52_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9778833.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3615..5321
                     /locus_tag="IWL52_002311"
     CDS             3615..5321
                     /locus_tag="IWL52_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9778834.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5463..6869
                     /gene="lacG"
                     /locus_tag="IWL52_002312"
     CDS             5463..6869
                     /gene="lacG"
                     /locus_tag="IWL52_002312"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9778835.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6892..7164
                     /locus_tag="IWL52_002313"
     CDS             6892..7164
                     /locus_tag="IWL52_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP9778836.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            7185..8084
                     /locus_tag="IWL52_002314"
     CDS             7185..8084
                     /locus_tag="IWL52_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9778837.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2677 a         1424 c         1738 g         2586 t
ORIGIN      
        1 caaaaacaaa tgttagatga tgaaataaga acagtggatt gacgtatatt agcttaggtc
       61 agattttgta taagacgaaa ataaagtagg acctcttaat cagtaagtta tagaaagtaa
      121 aagacttttg taatacttga atagatattt cacgtccatt ttgtgatgga ttaaatgaac
      181 aaaaatgaac aataatttaa cggtgttatc tattttttaa aaaaacaaat aaaaaaacaa
      241 aaaataaaca aaaaaagtgt tgctttttgt ttgaatgctt gatattatat aaacaaagaa
      301 atgatgaaaa cgttttttcg aacattttgc aaaatgtttt ctacttctac gtagcatttc
      361 tttctaaaat ttaggaggtg gtcaaaatgg ctattgttgt tggtgcagat ctcaaaggta
      421 cgagattaaa agatgttgtg aaaaattttc ttgtagaaga aggttttgag gtaattgatg
      481 tgactaagga cggacaagat tttgttgatg ttaccttggc agttgcctct gaagtaaata
      541 aagatgagca aaacttaggt attgtaattg atgcttatgg agctggtcca ttcatggtag
      601 caactaaaat taaaggtatg gttgcagcag aagtttcaga tgaacgctca gcttatatga
      661 cacgtggaca taacaattca cgaatgatta cggttggcgc tgaaatcgtc ggtgatgagc
      721 ttgctaaaaa catcgctaaa ggtttcgtca atggtaaata tgatggcgga cgtcatcaaa
      781 tccgagtaga tatgcttaac aaaatgtgtt aaggaaagga gttaaggaat gagaatcgca
      841 attggatgtg accacattgt cacagatgaa aaaatggcag tatcagaatt cttgaaatca
      901 aaaggacatg aggtccttga tttcgggaca tatgaccacg cacgtactca ctaccctatc
      961 tatggtaaaa aagtcggtga agcagtagta agtggtcaag cagacttggg agtatgtatc
     1021 tgtggtacag gtgtcggcat taacaatgca gtcaataaag ttccaggtgt tcgctcagcg
     1081 ttggttcgtg atatgacatc agctatatat gcaaaagaag agttaaatgc gaatgttatc
     1141 ggtttcggtg gaaagattac tggtggtctt cttatgaatg acatcattga agctttcatt
     1201 gaagctgagt acaaaccaac agaagaaaat aaaaaattga ttgcaaaaat cgaacacgtt
     1261 gaaacacata atgcacatca agcagatgaa gatttcttta cagaattcct tgaaagatgg
     1321 gaccgtggtg actaccacga ttaaggtata acaaatgatt ctgacagtca cactaaatcc
     1381 ttcagtagac atctcttatc ctctagaaac attgaaaatt gatacagtga atagggtgaa
     1441 agatgttagt aagacagctg gtggaaaagg cttaaatgtt actagagtct tgtatgaatc
     1501 tggtgataaa gttactgcta caggtttctt aggtggtaaa attggtgaat ttattgaaag
     1561 cgaattagaa caatctcctg ttagtccagc tttttataaa atctcaggaa acactagaaa
     1621 ctgtattgct atcttacacg agggtaacca aacagaaatt ttggaacaag gaccaacagt
     1681 ttctcatgaa gaagcagaag gttttcttga ccactatagc aatcttatta aacaatcaga
     1741 agttgtgaca atttcaggaa gtttaccttc aggacttccc aacgattatt atgaaaaact
     1801 tatccaactt gcttcggatg aaggagtagc agtcgttttg gattgttcag gtgcacctct
     1861 tgaaacagtt ctaaaatcgt cggctaaacc tactgccatc aaacctaata atgaggaact
     1921 ttcacagttg cttggaaaag aagtaacaaa agatattgaa gaactcaaag acgttcttaa
     1981 agagtcactt tttagtggta tcgaatggat agtggtctca ttaggtcgaa acggtgcctt
     2041 tgcaaaacat ggtgatgttt tctataaggt agatattcct gatatcccag ttgttaatcc
     2101 agtcggatca ggggactcaa cggtagctgg tattgcatca gctttaaata gtaaaaaaag
     2161 tgacgctgac ttattaaaac atgcgatgac attgggtatg ttaaatgctc aagaaacaat
     2221 gacagggcat gtgaatatga ctaattacga aacactaaac tcacaaattg gagtaaaaga
     2281 ggtataaaaa tggtacttac agaacagaaa cgcaaatctt tggaaaaaat ttcagataaa
     2341 aacggtgtta tctcagcttt ggcatttgac caacgtggtg ctttgaaacg tttgatggca
     2401 cagtatcaag atacagaacc aactgtagct caaatggaag aactaaaggt tttggttgct
     2461 gatgaactta caaaatacgc ttcttcaatg ttgcttgacc ctgaatatgg acttccagca
     2521 acaaaagcat tggataaaga agcaggtctt cttcttgcct atgaaaaaac aggttatgat
     2581 acatcaagta caaaacgttt gcctgactgc ttggatgttt ggtctgcaaa acgtatcaaa
     2641 gagcaaggcg ctgatgccgt taagttcttg ctttactatg atgtagacag ctcagatgaa
     2701 cttaaccaac aaaaacaagc ttatattgaa cgtgttggtt ctgaatgtgt cgctgaagat
     2761 attcctttct tcttggaaat ccttgcttat gatgaagaaa tctcagatgc aggttctgtt
     2821 gaatatgcaa aagtgaaacc tcgcaaagtc attgaagcga tgaaagtttt ctctgatcca
     2881 cgctttaaca ttgatgttct taaagtagaa gtaccagtta acgttaaata tgtcgaaggt
     2941 tttgctgatg gtgaagtggt ttacagtaaa gctgaagctg ctgacttctt taaagcacaa
     3001 gaagaagcga ctaaccttcc atacatctac ctaagtgcag gtgtatctgc taaattgttc
     3061 caagaaacat tgcaatttgc tcacgactca ggtgccaagt ttaatggtgt gctttgtgga
     3121 cgtgctactt gggcaggatc tgtagaacct tacatcaaag aaggtgaaaa agcagcacgt
     3181 gaatggttgc gtactactgg attcgaaaat attgatgaac ttaacaaagt tcttgttaaa
     3241 acagctagtc catggactga taaagtatag taaaaacata aaacggagga tattgttatg
     3301 aacagagaag agatgactct cttagggttt gaaattgttg cttatgctgg agatgctcgc
     3361 tctaaacttt tagaagcgct taaagcggct gaaaatggtg atttcgctaa ggcagatagt
     3421 cttgtagtag aagcaggaag ctgtattgca gaggctcaca attctcagac agctatgttg
     3481 gctcgagaag cttctgggga ggaacttcca tacagcgtta ccatgatgca tggtcaggac
     3541 cacttaatga ctactgtctt attaaaagat gtgattcatc acctcatcga actttataaa
     3601 agaggagcaa aataatgcat aaactcattg aacttattga gaaagggaaa ccgttctttg
     3661 agaaaatctc tcgaaacatc tatcttcgtg ctattcgtga tggatttatc gctggtatgc
     3721 cagtcatcct tttctcatct atctttatcc ttattgccta tgtcccaaat gcttggggat
     3781 tccactggtc aaaagacatt gagaccttct tgatgactcc ttatagctat tcgatgggta
     3841 tcctagcatt ctttgttggt ggtactaccg ctaaagcttt gacggactca atgaaccgtg
     3901 acctacctgc gaccaatcag attaacttct tatctacgat gctagcgtct atggtagggt
     3961 tccttttgat ggcagctgag cctgcaaaag aaggaggctt cttgacagcc ttcacgggaa
     4021 caaaaggtct tttgacagca tttattgcag cctttgtgac agttaatgtt tacaaggtct
     4081 gtgtgaaaaa taatgttact attcgtatgc ctgaagaggt tccaccaaat atctctcaag
     4141 tatttaaaga cttgattcca ttcactgtag cggttgttct cctttatgga tttgaactca
     4201 ttgttaaggg aactcttggt gtaaccgttg cagaatcaat cggtaccgtt cttgctcctc
     4261 tcttctcagc tgcagatggt taccttggaa ttacacttat ctttggtgct tatgccttct
     4321 tctggtttgt tggtatccac ggtccttcaa ttgtcgaacc agcaatcgct gctatcactt
     4381 atgccaatat cgatgccaac ttgcatctta tccaagctgg acaacatgca gataaagtta
     4441 tcacttctgg tactcaaatg tttattgtta ccatgggtgg aacaggagct acattgattg
     4501 ttccattctt gttcatgtgg atttgtaaat cagaacgtaa ccgtgccatc ggacgtgcct
     4561 cagttgttcc aacattcttt ggggttaatg agccaatctt atttggtgca ccaatcgtat
     4621 tgaatccgat tttctttgta ccgttcattt tcgctccgat tgtcaacgtt tggatcttta
     4681 aattctttgt tgacacattg aacatgaact cattctctgc caaccttcca tgggttactc
     4741 ctggtccgtt gggaattgta ctcggtacta acttccaagt tctatcattt atcttagccg
     4801 gactcttggt agttgttgat actatcattt attacccatt tgttaaggta tacgatgaac
     4861 aaattcttga agaagaacgt tctggtaaaa caaacgatgc tcttaaagaa aaagtagctg
     4921 caaacttcaa tactgctaaa gcagatgccg ttcttggaaa agcaggcgtt gctaaagaag
     4981 atgtagcagc aaacaataat atcacaaaag aaactaatgt ccttgttctt tgtgcaggtg
     5041 gaggtacaag tggcttgctt gccaatgcct tgaacaaagc agcagcagaa tacaatgttc
     5101 cagtcaaagc ggcagcgggt agttacggtg ctcaccgtga aatgttgcca gagtttgact
     5161 tggttatctt ggctccacag gtcgcttcaa acttcgatga tatgaaggct gaaacggata
     5221 aattgggcat taaacttgca aaaactgaag gagctcaata tatcaaattg acacgtgatg
     5281 gacaaggtgc tcttgcattt gttcaacaac agtttgatta ataaatttgt aaaagttatt
     5341 tgagacgaga ttattctttc agttccttat aatattgtta accatatcat tgcatttcat
     5401 ttccttggtt tccggaactc agaatatcgt ctcaaatgct ttttttgaaa ggatttatag
     5461 ttatgactaa aacacttcct aaagatttta tttttggtgg tgctacagct gcttatcaag
     5521 cagaaggtgc gactcacacg gatggtaaag gcccagttgc atgggataag tatcttgaag
     5581 acaattattg gtacacagca gagcctgcaa gtgattttta ccataaatat cctgttgatt
     5641 tagaattagc tgaaaaatat ggcgttaatg gtattcgtat ttcaattgcc tggtctcgta
     5701 ttttcccaac tggctatggc gaagtaaatg aaaaaggtgt tgaattctac cataatcttt
     5761 ttgccgagtg ccacaaacgt catgttgagc cattcgtgac tttgcaccac tttgacacac
     5821 cagaagttct tcactcagat ggagatttct taaaccgcga aaatatagaa cactttgtag
     5881 attatgccgc tttctgtttt gaagagttcc cagaagtaaa ctactggaca actttcaatg
     5941 aaattggccc aattggagat ggtcaatact tggttggtaa attccctcca ggcattcaat
     6001 atgatcttgc caaagtcttc caatcacacc acaatatgat ggtttctcat gcacgtgctg
     6061 tgaaattgta taaagataaa ggttataaag gcgaaattgg tgtagtacat gcgttgccaa
     6121 ccaaataccc ttatgatcca gaaaatccag cagatgttcg tgccgctgag ttagaagata
     6181 tcatccataa caaatttatc ttggatgcaa cctaccttgg acactattca gataaaacga
     6241 tggaaggggt caaccacatc ctagctgaaa atggtggaga acttgatctt cgtgatgaag
     6301 acttccaagc acttgaagca gctaaagatt tgaacgattt ccttggtatc aactactaca
     6361 tgagtgattg gatgcaagcc tttgatggtg agactgaaat cattcacaat ggtaagggtg
     6421 aaaaaggaag ttctaagtac cagattaaag gtgttggtcg ccgaatcgca ccagattatg
     6481 tgcctagaac ggattgggac tggattattt atcctgaagg cttgtatgac caaatcatgc
     6541 gagtgaaaaa tgattatcca aactacaaga agatttacat cactgaaaac ggtcttggat
     6601 acaaagatga gtttgtagat aataccgttt atgatgatgg tcgtattgat tacgtgaaga
     6661 aacacttgga agttttatca gacgcaattg cggatggtgc aaatgttaaa ggttacttca
     6721 tctggtcact tatggatgtc ttctcatggt caaacggata cgaaaaacgt tatggactat
     6781 tctatgtaga ctttgacacg caagaacgct atcctaagaa atcagctcat tggtataaga
     6841 aagtagcaga aactcaagtg atagagtaat attacttgaa aatgtaaaaa attgttatcc
     6901 gatactttag aaaaagtaca acacttattt agaccaggct ttgaaaaggt atatattgtt
     6961 agtttcgaag attgtcctat gattcctgag cttgaagcta ccccgttgct aaaatgtggt
     7021 aaatggtatg tttcaactgg aaaagagtgg atttgccatt ctgatttaga gttatcagca
     7081 tttgaatggg aatttttaca atcactcgat gtagaaatac gtgagactat tcacttcgag
     7141 gtaaattatt tgcctttcca ataataaaaa gaaaggaatt agttgtgact atcgaattaa
     7201 aaaacgagta tcttactgtt cagtttaaaa cacttggtgg tcaactgact tcgattaaag
     7261 acaaggatgg gatagagtat ctctggcaag ctgatccaaa ttattggaat ggacaggcgc
     7321 ctattctatt tcctatctgt ggtagtttac gaaatgattg ggctatttat agacctcaag
     7381 aaagaccttt ttttacaggt cttattcgta gacatggttt tgttcgaaaa gaagaattta
     7441 ttctggaaga agtcaatgat aatagcgtga cctttagtat taaaccaaat gctgagatgc
     7501 ttgataatta cctatatcag tttgaactga gagttgttta taccttgaat ggtaaatcaa
     7561 ttagaaccga atttcaggta acgaacttgg aaatggaaaa aacaatgcct tactttattg
     7621 gagcccatcc agcattcaat agtcctttag tagaaggtga aaactatgag gattattata
     7681 ttgaatttag tgaggttgag tcttgctcga taccaaagag ttttcctgag acaggattat
     7741 tagatttgca agatcggaca ccttttttgg aaaatcaaaa aattctcgat ttggactact
     7801 cacttttttc ccatgatgcc attacacttg accagctcaa gtctcgtagc gtaacgcttc
     7861 ggtcaaggaa gtctggtaaa ggtcttcgag tagattttga tgattttccg aacttgattc
     7921 tgtggtctac aactaataaa agtccattca ttgctttgga accttggagt ggtctctcca
     7981 cttcacttga agaaggtaat ttcttagaag ataaaagaca ggtaacgaaa atttcacctc
     8041 aagaaacttc tagaaaaatc tatgatatta ctatccttaa ttaaaatttg ttattatttg
     8101 agtcttcaat aagatttgaa tactcttacc ttgtgatgat agattggtct tctccgtgcc
     8161 agtcttacat cgcaaggttt ttaagttttt tgtgggtcta tgtcaactgt agtgggtgac
     8221 gaaaagctaa tatctaaaga cagtctggta gtaaaaaata agattattta ctacttcttg
     8281 atttaaaata atagtggagc cattaatttg tggcaaattt aatttaatat tttgcctcaa
     8341 attagccaca ctttttatag aactaagagg aaaaatgaaa ttttcttcct attaatatag
     8401 ttgtttgaaa tcttaaatat agcgt
//
LOCUS       DACDVT010000093         8387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDVT010000093 DACDVT010000000
VERSION     DACDVT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.095"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(134..928)
                     /locus_tag="IWL52_002315"
     CDS             complement(134..928)
                     /locus_tag="IWL52_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP9778838.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1547..4381
                     /locus_tag="IWL52_002316"
     CDS             1547..4381
                     /locus_tag="IWL52_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9778839.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4572..5063
                     /locus_tag="IWL52_002317"
     CDS             4572..5063
                     /locus_tag="IWL52_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9778840.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5166..6155
                     /gene="manX"
                     /locus_tag="IWL52_002318"
     CDS             5166..6155
                     /gene="manX"
                     /locus_tag="IWL52_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9778841.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6188..6991
                     /locus_tag="IWL52_002319"
     CDS             6188..6991
                     /locus_tag="IWL52_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9778842.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7012..7926
                     /locus_tag="IWL52_002320"
     CDS             7012..7926
                     /locus_tag="IWL52_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9778843.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8009..8383
                     /locus_tag="IWL52_002321"
     CDS             8009..8383
                     /locus_tag="IWL52_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9778844.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
BASE COUNT         2725 a         1386 c         1734 g         2542 t
ORIGIN      
        1 aatttaaacc ataattgtgt ttttccttcc attaacatat aaagttcacc tcaaaaagag
       61 tatacgaaca aatgtttgta ttgtaaatag aaaaagatga gctaaattaa atgcccatct
      121 tctttgatag atattataaa tctaataatt cttgtttctt tttattaaat tcttcttctg
      181 taatgattcc atcatccaat agttctttat attttcttat ttctgaagct gaggaaattg
      241 ttgatgtatt ggaatttgta gcagaaatat ttcttctttt taaattataa ataatttcgt
      301 ttaaagaatc acttatccta tcaacagtat ctttatcaac tgcaacattc aatactttac
      361 caagagtatc aatagttaat gtacccaaca gcactcctct ttttttacta atattattta
      421 aattgtctaa tataatactt tgtacattat tgcctatcat tttttgttga gctacaatta
      481 aacgtttatt agttaatgcg tatgcataat tattgtcatg cttagttgat gagatataat
      541 tatgcaatcc tacaaagcag aagattacgt attcgtcact gttaagttga tctgatatta
      601 agttaaaatg atgagttgtc cattttttag tcattccttt accgtagccg tatttaagac
      661 aaaattctac catctcttca gtggttctca ttctatcatt acctatatca tgattgtttt
      721 gtgcagtatg atcagatatg tttaattttc ttttttctga tggatcctta tctaaatctt
      781 ctaaattcaa agatcttatt attttataag gagtagtcat accgtaacca gcttttttca
      841 tacaattgcc gcaaacaatt tgtttatctt tgattttata tgaagttaag ccaattttac
      901 ctccgcactt tgcacattgt ttactcacat tagttcctcc tagatatcag ctttctacta
      961 ctattaacat tctatcaata gtgaccatac tttgaccata ctttttagaa agaatagaaa
     1021 attccaagat atttaataat agaagaaaag cactaacatc atgggttcag aagaattgag
     1081 agcttatcag gaaacagtat aattgaagtg tctgtttatc cagtagctca agaagcttaa
     1141 ttgaatctta tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata
     1201 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
     1261 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
     1321 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
     1381 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
     1441 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
     1501 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
     1561 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
     1621 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
     1681 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
     1741 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
     1801 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
     1861 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
     1921 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
     1981 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
     2041 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
     2101 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
     2161 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     2221 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     2281 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     2341 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     2401 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     2461 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     2521 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     2581 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     2641 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     2701 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     2761 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     2821 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     2881 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     2941 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     3001 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     3061 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     3121 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     3181 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     3241 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     3301 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     3361 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     3421 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     3481 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     3541 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     3601 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     3661 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     3721 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     3781 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     3841 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     3901 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     3961 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     4021 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     4081 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     4141 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     4201 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     4261 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     4321 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     4381 aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     4441 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     4501 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     4561 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     4621 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     4681 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     4741 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     4801 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     4861 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     4921 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     4981 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     5041 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     5101 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     5161 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     5221 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     5281 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     5341 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     5401 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     5461 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     5521 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     5581 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     5641 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     5701 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     5761 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     5821 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     5881 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     5941 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     6001 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     6061 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     6121 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     6181 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     6241 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     6301 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     6361 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     6421 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     6481 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     6541 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     6601 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     6661 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     6721 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     6781 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     6841 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     6901 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     6961 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     7021 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     7081 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     7141 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     7201 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     7261 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     7321 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
     7381 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
     7441 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
     7501 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
     7561 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
     7621 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
     7681 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
     7741 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
     7801 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
     7861 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
     7921 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
     7981 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
     8041 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga
     8101 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
     8161 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
     8221 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
     8281 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
     8341 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaa
//
LOCUS       DACDVT010000094         7858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDVT010000094 DACDVT010000000
VERSION     DACDVT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.096"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            170..514
                     /locus_tag="IWL52_002322"
     CDS             170..514
                     /locus_tag="IWL52_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9778845.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IWL52_002323"
     CDS             515..934
                     /locus_tag="IWL52_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9778846.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="IWL52_002324"
     CDS             complement(1026..1376)
                     /locus_tag="IWL52_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778847.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IWL52_002325"
     CDS             complement(1342..2673)
                     /locus_tag="IWL52_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9778848.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IWL52_002326"
     CDS             2929..3495
                     /locus_tag="IWL52_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9778849.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IWL52_002327"
     tRNA            3640..3727
                     /locus_tag="IWL52_002327"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IWL52_002328"
     CDS             4061..5035
                     /locus_tag="IWL52_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9778850.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IWL52_002329"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IWL52_002329"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9778851.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..>7858)
                     /locus_tag="IWL52_002330"
     CDS             complement(7450..>7858)
                     /locus_tag="IWL52_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9778852.1"
                     /translation="NLDKLTRKKAIDIANALLDEGYPDNRAIPIAIDQAKKWYEDASE
                     SEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKSKGAKKASNHFDTK
                     KEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT         2744 a         1319 c         1502 g         2293 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttt
//
LOCUS       DACDVT010000095         7843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDVT010000095 DACDVT010000000
VERSION     DACDVT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.097"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2008)
                     /locus_tag="IWL52_002331"
     CDS             complement(<1..2008)
                     /locus_tag="IWL52_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9778853.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2024..3020)
                     /locus_tag="IWL52_002332"
                     /pseudo
     CDS             complement(2024..3020)
                     /locus_tag="IWL52_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3218..4459
                     /gene="sstT"
                     /locus_tag="IWL52_002333"
     CDS             3218..4459
                     /gene="sstT"
                     /locus_tag="IWL52_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9778854.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="IWL52_002334"
     CDS             4606..5241
                     /locus_tag="IWL52_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9778855.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="IWL52_002335"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="IWL52_002335"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9778856.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6317..7696)
                     /locus_tag="IWL52_002336"
     CDS             complement(6317..7696)
                     /locus_tag="IWL52_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9778857.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT         2586 a         1222 c         1474 g         2561 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaaag aaatataaaa aaagactttt tagcctgttt atgtgtacag ctaaaaagtc
     6301 tttcttaaat aaaaatctag tgagaatgtt gtacttctct tctcggttgt ttttgtttag
     6361 aaaaagtaaa tcgataaagt aaaaataaaa tcaaagtaac gatagataaa tatcttgaag
     6421 caattgctag ttttgtgtac tgataagtaa taacgacttt atttatacct ggaggaatag
     6481 ttaattcagt tgtgccaaac ttagataatt ttgtttgtac tagtttgcca ttaatagaag
     6541 ccacttgccc tttataacga taaataggtg tcgtcaagac agtattttta ttattcgaat
     6601 tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt atttcctggt
     6661 tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac tctttgtttg
     6721 cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt gctataagtg
     6781 ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc aatattagtg
     6841 caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa atataataga
     6901 tcactatact catcgtatag gctataaata aagaagaaaa accatttaac cgccagacaa
     6961 attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa ttggttgaac
     7021 aaaaaagaac aaatacacca agaaagaaaa taaataaatc atcagcagtt aattttttta
     7081 taaaaataaa agcaccaatc aatccgagaa ataagaataa tccaatagta tagttgttat
     7141 agttattatt taaaatgtgt gtcagcatat cactaggagc cataccactt aattcttttc
     7201 ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg
     7261 cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt
     7321 ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga
     7381 cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag taccattttt
     7441 ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag
     7501 cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg
     7561 tataaataat cgaaaataac agcgaaatat aacgattgtt ttttatttgt ttcatagaaa
     7621 aataagaaac gaccattgtt aaaaaagtga taaaaaaata gtagatatta taacttaaaa
     7681 caagatttcc catcatttta taaaataaaa aagctggata aagagtaagc catggataaa
     7741 aaatattcat cattgttcca tgatgatcga agttattgaa gtttactgga gaactccata
     7801 cattatctag tcctatgatt cgagataaat gaaagtaagt atc
//
LOCUS       DACDVT010000096         7619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDVT010000096 DACDVT010000000
VERSION     DACDVT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.098"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            225..593
                     /locus_tag="IWL52_002337"
     CDS             225..593
                     /locus_tag="IWL52_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9778858.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            773..1714
                     /locus_tag="IWL52_002338"
     CDS             773..1714
                     /locus_tag="IWL52_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9778859.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1783..2514
                     /locus_tag="IWL52_002339"
     CDS             1783..2514
                     /locus_tag="IWL52_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9778860.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2658..4838
                     /locus_tag="IWL52_002340"
     CDS             2658..4838
                     /locus_tag="IWL52_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9778861.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4858..5340
                     /locus_tag="IWL52_002341"
     CDS             4858..5340
                     /locus_tag="IWL52_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9778862.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5356..6117
                     /locus_tag="IWL52_002342"
     CDS             5356..6117
                     /locus_tag="IWL52_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9778863.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6248..7582
                     /locus_tag="IWL52_002343"
     CDS             6248..7582
                     /locus_tag="IWL52_002343"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9778864.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2565 a         1319 c         1665 g         2070 t
ORIGIN      
        1 tgtttttagt cacgacctct ttttttattt attccatata aatacacatt atttattccg
       61 aaaagtgaaa gcgcaaataa caaaaacaac caacttattt attattaaag aaaagcgtat
      121 ttaagtagga cgattctgtt gagagctttc gtagattttc ccattttcct atggtaaact
      181 gacaaagttg agaatgttag aacagatcgt ttaggaggaa acagatgttt acgaatgaaa
      241 taaaaatcat gctttatgtg gacgatgtag aaaaaaatgc aacgttttgg agagcagttg
      301 gctttgcgga aaaagatcgt caagaaatgg atgggacgct tataattgaa gtaggagttg
      361 aggacagtca aacctcattt ctgttatatg atcgagcatt tgttgagtcc cattcacctg
      421 gcacagctac tagtgcccca tctttgatgt tttacagtaa agatatcttt ggactatata
      481 aaaaaatgca gacgcaatca gtcacattag gtgatatggt acagcttgga gaagaatatg
      541 tctttaactt tgctgatccc gatggcaact actttgcggt tacaggtaaa taatagaaat
      601 aaagactttg tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat
      661 attatgtaaa caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg
      721 cgaacgcctt ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta
      781 tcaagtactg ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa
      841 tcatttagcc ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg
      901 aataaatggt actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa
      961 agctgatcct cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt
     1021 taagaagatg tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat
     1081 tgatccaaac caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt
     1141 agatgaacag acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt
     1201 tcgaggggct gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga
     1261 gcacaaagaa gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg
     1321 cgagaagttt tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg
     1381 cggtattgct acttatggta aggatcctga agtccaagga gaattatggg acggcaagat
     1441 gtatgtgttt gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg
     1501 tcgcgactgg tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg
     1561 caatcgtcag attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat
     1621 agagtgtgcg aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga
     1681 agcagaactg cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca
     1741 tttccaatat gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat
     1801 gctattgggg atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat
     1861 ccaaagatcc atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa
     1921 gaatgttttt tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga
     1981 aaccatgaag aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat
     2041 atgagaaatg gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa
     2101 tactccccaa ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt
     2161 ttagtccagc gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg
     2221 gatttgacaa agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa
     2281 ccatttcatg aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca
     2341 ccaatgctcc atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga
     2401 atggacggtg gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt
     2461 attcttcaag atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg
     2521 gaaagaatga aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt
     2581 ttctttcagt agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc
     2641 agaaaagagg cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa
     2701 aagaactttc agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag
     2761 gaaacactgt tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag
     2821 tccagattcg tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa
     2881 ctgaagtgat tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa
     2941 tcacccaagc cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac
     3001 gacggacaaa agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga
     3061 tgcagcttac agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag
     3121 aagtctcttc tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca
     3181 gcgataatgc aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg
     3241 tcagcaatgt caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact
     3301 ttgcagaacc cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa
     3361 aagaagatat tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg
     3421 atcgccaaat agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc
     3481 aggatagtta taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga
     3541 ctgaaaaggc agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt
     3601 tgcaaccgcc actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat
     3661 gtaaactagc agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc
     3721 ataaacctgc agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta
     3781 ataaatatca agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac
     3841 tttttgtggc tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg
     3901 aagcaggcgc ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac
     3961 aagttgaaga aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat
     4021 tagtcaaaat cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa
     4081 aacgtttggc tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg
     4141 atgtgaatac agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg
     4201 cccaaaacat tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga
     4261 aaaaagtgcc acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc
     4321 ctggtgggaa aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa
     4381 aagaaatatt aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag
     4441 aaaaattgac acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa
     4501 cgttagccga tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc
     4561 cagcgccctt attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat
     4621 tgactggaac ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc
     4681 aagacggact tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg
     4741 tcgtgtcagt aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggta
     4801 gaatcagttt gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg
     4861 acacaagaag aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct
     4921 ttcaagcacc aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta
     4981 aacgatcatc atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt
     5041 gcaggaatca tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat
     5101 cagcataaag atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca
     5161 ccagccttga cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc
     5221 acgatctgcc gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga
     5281 gaaatcaaat ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga
     5341 aaggatagaa ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta
     5401 ttaaattttt tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt
     5461 attttagctg gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat
     5521 ggccaagtag atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat
     5581 gagaattttg aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt
     5641 cgtcagtatc taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag
     5701 tctacaaatt ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct
     5761 gtaccagaaa aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact
     5821 tttgagaaag tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg
     5881 gaaatccttc acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg
     5941 cctaaggaat atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag
     6001 aacggctatg gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt
     6061 tggtcttctt acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca
     6121 ttttagcaag cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa
     6181 gataatgagg agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag
     6241 gattcaaatg gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat
     6301 gagctccttg gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga
     6361 aaaatatttc tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa
     6421 cgcagataat gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca
     6481 tgaagaaatc cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat
     6541 tggtcatttt atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag
     6601 tacgactgga ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg
     6661 agatggaaca ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata
     6721 tcgccgtcat ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga
     6781 tcatccggat tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg
     6841 acaagtgaaa aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc
     6901 aaatgtgccg atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat
     6961 cgaacgcaca acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca
     7021 cttcactgta ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt
     7081 agcttatttc gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg
     7141 agtaaaacgt cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc
     7201 gcatcaccct gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa
     7261 agaaataatt gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga
     7321 atttgcagaa gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc
     7381 tcgggaagaa caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg
     7441 agaagtcatc aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat
     7501 cttcatgggt gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag
     7561 cacgacgaaa aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagag
//
LOCUS       DACDVT010000097         7416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDVT010000097 DACDVT010000000
VERSION     DACDVT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.099"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..496)
                     /locus_tag="IWL52_002344"
     CDS             complement(<1..496)
                     /locus_tag="IWL52_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9778865.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADG"
     gene            581..757
                     /locus_tag="IWL52_002345"
                     /pseudo
     CDS             581..757
                     /locus_tag="IWL52_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(933..1502)
                     /locus_tag="IWL52_002346"
     CDS             complement(933..1502)
                     /locus_tag="IWL52_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9778866.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1576..1899)
                     /locus_tag="IWL52_002347"
     CDS             complement(1576..1899)
                     /locus_tag="IWL52_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778867.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2053..2865)
                     /locus_tag="IWL52_002348"
     CDS             complement(2053..2865)
                     /locus_tag="IWL52_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778868.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3219..4067
                     /locus_tag="IWL52_002349"
     CDS             3219..4067
                     /locus_tag="IWL52_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9778869.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4212..5153
                     /locus_tag="IWL52_002350"
     CDS             4212..5153
                     /locus_tag="IWL52_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9778870.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5143..7314
                     /locus_tag="IWL52_002351"
                     /pseudo
     CDS             5143..7314
                     /locus_tag="IWL52_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2213 a         1474 c         1332 g         2397 t
ORIGIN      
        1 tcccatcagc tcttactcta gctagttctt ttccagtatt gtagtccata atgaaaatat
       61 actcgtattt ataattagca atgtgccatc cagctacatg caagtttgcg ttttcgattt
      121 ctccgaactg atcaatgtgg gcgtaatttg ttccatctgt cagcgtagaa tttgcagcac
      181 ctgctcgagt tggatcaatt actggtttat cctccgaagt agttggatta tcatccgtaa
      241 aaccatgagc caaatcatag gctagttttt ctttacttac acctatttca gaaagataac
      301 cgtaaggatc tgtatgatcg ccccaaatat tttgtgttac ccataaatgc gatttgattc
      361 ctggttggtt ataaggagtg tctaatgtta atggaatacc atatttcatt gctgaatctc
      421 ttgccaattc aacgtatact ttatagttct tttcaaaagt ttctttatca tgtgtgtgtt
      481 gtaactcgat ttgcacagga cttttggctc acgaggttgt cctactctac atcttgctga
      541 tagatatgct gaaaggaaaa caccaacatt atgttttgct ttttcagcta gaacgagaca
      601 agcaggaata cctttagaag atatcaaagg ttttttaggt cataaagatg tttccactac
      661 acaagtctat gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc aactagaaaa
      721 ctatatagaa gagcaaataa aaaagcactc aaattgagtg cttttttaag ctcactatca
      781 ctaaaactat cactaatttt atttataagc gaacatgttt ctatcactga ttttttaaat
      841 ctagcgaaaa acccttatct taaaggcgtt cgttcaattc tttggcaagg tcttcaaagc
      901 ctggcttgcc tagaagtgcg aacatggttt tcttatagct catgtagaaa aattttcttg
      961 atgatgattt tatccatcaa ttgagattga ctgttttgct ctcctatgat ttcagctatg
     1021 attgtccgat tttgcggttt tcttcttaac tggtagttga ttttcccagt ttccatcaac
     1081 tcgctaagca tatccacgac tccgccaaat ccggtctttt tcgtaataaa acctagtgct
     1141 accattcgtt catctagatt ttttgctgta atcgtcacac cagtaatgag cgagtgctgt
     1201 aatgtttggg ccagctcttc catcccttca gcattggata cgacggaagc tgttttgtta
     1261 ataggttctt cgtttatttc ttttggttct ttcctctcca ctttcactgt ttgacgaagg
     1321 acatctagtt cttgcaagag cgtttcgtat tgttcttggg tcagcatcac gccagctact
     1381 ttttcacgat taaatatata tacgccagtt cccgcgtctc gcgcttgagc aaatacatcc
     1441 atcggcgatc gtttcacttc tgtgatcgaa gatgtgggaa cttctaattt ctttaattcc
     1501 aatggatttc ctcctttact aatcgctttt tattatagca cagactgact tgttttgatc
     1561 ttttttccaa aggttttatc ggtttttgcc taaccagtaa ttgatatcgg taagaagtct
     1621 ttcgatacca aatgaaggat ggtacagata cgttttttga tcttcgttat ggtctagtgg
     1681 aaaaaaatga tcgatgatca ctagatctgc ataccgaggc ggcttatttc gagtacatac
     1741 atgtatagag atccgttttc gatgtcttag cgttttgttc agtaccccag caagtcttct
     1801 tgcttcttgg tgttcatagc gaaaaaaaaa gacgattttc acttcaggag ggtggacatc
     1861 taaataccct cgttcctgga gtgttttcag taaaagcatc ccccactcta ctgcggggtg
     1921 tgcaagtgct tgttggatcg ctagccgaaa ttgatatagt ttatctttgg aattgggaaa
     1981 tttttctaat gtttcctgat aataacgaac ttcttctatc agccggtcat ggataccaat
     2041 tacgtaacga agtcattctt ggtaaagtac ttttgtcagc tttgatttgc tttcgctctc
     2101 aaattcgcta ttcagccaaa acagcgaatc aagcttttca gtcacaatcg tcaatttccg
     2161 atagtcttgt gtctcttcta tccgtttcca ccgcttttca gtaatctggt tacagctcaa
     2221 gcgcaaaatt gctagataat ttttttcttt tattggcaaa atctgctcct ctctaaatag
     2281 caattcagga aacaattcaa agaatagtgt tcgactgtct cctaaaatct cgatttctgc
     2341 cggatccttt ctttgcttca ataattggat gaacatctca gaaaaggact gctctccatc
     2401 atctgcaaaa aaatgcggaa aatctttcaa aaagcaaaac agcagatatc ttacttttct
     2461 ttctgaacca aagatctgtg cttttttata agaaaaagaa aggtggtact cgccaaacca
     2521 ctttctcaat cgctcttgcg tagaacgatc gtacttctgc tcgtaaggtt cattcaatag
     2581 cagatttttc agtaaacaaa acgggggtga ttggatcaga aaaacaaaca agagataagg
     2641 ttcgctatat ccttgatcat aaactaactg aacggttttc ttatgaagaa tcagctgaat
     2701 gggacagtcg ctttccgcca aacgtttcag atgccgatcc actgttttaa catcgatttt
     2761 cagttcttca gctaaatcag cttttcgcca gatcaaatct ttcattagat tgattagtga
     2821 aaataaattc tgtgcatttt ttgataatag tttttgccat ctcatcgatc atttctcacc
     2881 tgatttctaa acatattgtt tgatcaaaat gggttcactc ctaaaaagat aaaaaactcc
     2941 ctttctattt atttaaatac gcaaatcttc ctcattattt tttattttac tcaatttaat
     3001 cgtattctca tttcaaaacg aatgtacttt aaaaaaacat aataagatca ctttgcttgt
     3061 aggatgtatt cccttccatt acaatgaaat aaatagcgat tttatgagaa ggtgatgtga
     3121 aaatggaaga aaaaatagaa gaatccaaac aaaaaatcga tcagttgata taagaattag
     3181 aaaaagcggt catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag
     3241 gtggtcatgt cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac
     3301 tagcaaaagc tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta
     3361 gtgatattct tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg
     3421 gaccgatctt taccacgatc ttgttagcag atgagatcaa tcgtacgact cctcgtactc
     3481 agtcagcact tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc
     3541 ctctagacaa tcattttttt gtcttagcta cgcaaaatcc aatcgagtac gaaggaacat
     3601 atcctttgcc ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt
     3661 cttttgatga cgaacttttg ctacttttgg ataaattcga aaaagtgttg gataacttaa
     3721 atgaagtatt gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg
     3781 tcagtcccga agtggcatct tatgccttgc agttagtgtc agcaacaaga agtcatgctg
     3841 ccatccagct tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt
     3901 atgctttaat ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt
     3961 acgtgttttc ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaagaaac
     4021 aaatcattca tactcttgtt gcacaaattc ctattcctgt gaggtagatt gaggtagaca
     4081 atggaaaaaa tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt
     4141 gtgctttttg ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact
     4201 ctttatgtat catggagctt gatttcatta agcagctttc taaagaaact ttcggttact
     4261 tgtacattgc agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca
     4321 aaaaagagaa ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt
     4381 gcagaaaaaa acaagagatt ttgattctta caaataaacc gcaagaagtc caattgcttt
     4441 ggcagccaaa agaaagagga tgccatgaaa cattaactgt catttttaca gcctatgata
     4501 gtctaggact tttcagaaaa agtgcaaaaa gagagatctc ttttcctgtg actgttctgc
     4561 ctgctttttg taagattcaa gcagaaaaat tacagagagt tctggaaaaa aaacagcagc
     4621 tgaacgcgta ccaaagaggt cttgattttc gggaacatcg cgcttatcgt ccaggtgatt
     4681 ctttcaaccg aatcgattgg aaattgtctg ctcatactca agagtggtta taccgtgaat
     4741 acgattatca agaagaagaa tgttctcctc ttttttgctt ttggggttcg cccagtccaa
     4801 aatttgaacc tctgctagac ttgtattttt ctttatggca tttgagaaaa gaaaagcagc
     4861 cggaacttct gatattaggc aaccgcgcct ttcatggcaa agaccccggt catacctatt
     4921 ttgcttcttt ggaagctggc gatgagaaaa cttttttcgc acacttgaaa aaatatgcaa
     4981 aaaaacaaat cgtcctcttt gttcctgaga attctcctga agtctcagaa gctattgaaa
     5041 cattgtcaaa agatcgaacg gtttatcttg tttatttcgt agaaaaacaa ctcatggtcc
     5101 aagaaaaaga caagatatgt tcattgccag gaggtgaatg gattgacgat taaacgggaa
     5161 aaatggctgc gtggcgttta ctcctttgtg atactaagtg taggtgctcc gatatttgta
     5221 gtagctaatc aattgcaaaa cagctacctt cttatcctaa ctattggtgt cctatgcctt
     5281 attgtctgct gtattcctaa aaaatgggta cgtttgctgc tttctttgcc agtaatgata
     5341 ggttgtttgt ttttatattt tccttcgaag acattttcca tattatggat cttacagttt
     5401 ttttcgtcag ttacagagga attcttccat ctatcccaag gtaaacttta ttatttgcca
     5461 gaaaagacag ctttttttct cataatgttg ctgatctatt tatttattac attgaccgtg
     5521 gattacgact attggtacgc accctttctt tctttgatgg cttacttcat gatgcttacc
     5581 gtttttcgga aaaaagattt actgtacgaa atgattgcgg tagtaactgt tttgttcgtg
     5641 tatctggcag caaggcaatt tttctccatc cggttgttgt cgagaaacac tcgattccag
     5701 tcattgacta cagttctgct tatttgtttc ttaactttta gcgctatctt gcctttatat
     5761 cttccgaaag tacaccaatc tctcgaggaa acgagtgaac cgatccgaga atatcttaat
     5821 gatgaagggc tttatgacac actccatgag tatcaattaa ccggaagtgc aagaacaggc
     5881 ttcagtgaga atgatgaaca gcttggtgga cctttatatg ataatggcga agttgttttt
     5941 acagcgaacc aaaaaggaaa tggctattgg aagatcgaaa ataaaaatca ttacaccggt
     6001 tctggatgaa tagaagatca ggacgcaccc atccaaccaa tccaagaatt ccctgttgac
     6061 atcacgagct accaaaccgt tatagaagac aaagaaaaca ttacattgac cctaaaaaaa
     6121 caagctttct taccttatcc ttatggaaat actaccttga ctgatttttc attagctagt
     6181 ggacaaacgc tttactattt gcctgaaagc cagcgattca ttcctgtccc gaatgaatcg
     6241 acacgttctg caactttaca aacagaaagg ttagttttta cgagagaaga attaaaaagc
     6301 agtcagttaa aagctgatga tttagaaaat cagcagacaa atcttcaatt gccaaaaagt
     6361 ttgcctaaac gtattgagga cttggcggtc gaattgacca aaaatgaagc aactttaatt
     6421 cgtaaagtcg aagctgtcta acagtattta aaaacaagcg gaaatttccg ctattccaaa
     6481 acggataccg ggcatactcc aaaagaacac gattatgtgg attatttcct ctttgattca
     6541 aaagtcggct attgtgacaa cttttcaact tctatggtcg tccttcttcg aacacttggt
     6601 atcccagctc gctggacaaa aggttttaca aatgggctca gaacaggcac agataatcaa
     6661 gggaatgagg aatacagcat tctcaatagt catgctcatt cttggccaga ggtatacttt
     6721 cctggatttg gctggcttcc atttgaacca accccttcat ttgccaatcc aatgggttca
     6781 caaccagttg ctgaaacaac cgaaaccgtc cagcctgcta tcgaacaacc aaatgaaagc
     6841 gaaaatatgg cagaaactat ttccagtgag agatctgagg tcattccatc agaagaaaca
     6901 acactcacac cagcttataa aatcgtctta ttcagccttg gagtgtgttt atccgtcttg
     6961 ttgttctttc tattgttatt cagaaaatct ctgcgctgga caagttatct tatctatctt
     7021 catttttttg cttctactaa tacgattgca tatcaaaaac ttcttaaccc tttggaaaca
     7081 ttggtaccgc gcaaacccgc agaatctttg caggaatata gcctgcgaat agaacaagaa
     7141 ttcaagctag atctgcgttt cattcgatta actactcaat atgaagcaga aatatatgga
     7201 cgacaagcag atccggtaaa attcgataaa aaaacctatt tagaaataac gcatataata
     7261 agaaaaacgg tatacaaaaa aattctgaca aaagggagcc gcttcaaaaa ataagataaa
     7321 atttctggag aatctacttc tcgaatgccg agaaccagct gattgagtat cgattactcg
     7381 aaaaaaaggt tgtgacacgt tttttgtcac aacctt
//
LOCUS       DACDVT010000098         7380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDVT010000098 DACDVT010000000
VERSION     DACDVT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.100"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..208)
                     /locus_tag="IWL52_002352"
     CDS             complement(<1..208)
                     /locus_tag="IWL52_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778871.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDS"
     gene            complement(326..586)
                     /locus_tag="IWL52_002353"
     CDS             complement(326..586)
                     /locus_tag="IWL52_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778872.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(723..1136)
                     /locus_tag="IWL52_002354"
     CDS             complement(723..1136)
                     /locus_tag="IWL52_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9778873.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1592..2077)
                     /locus_tag="IWL52_002355"
     CDS             complement(1592..2077)
                     /locus_tag="IWL52_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778874.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2216..2431)
                     /locus_tag="IWL52_002356"
     CDS             complement(2216..2431)
                     /locus_tag="IWL52_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9778875.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2655..3455
                     /locus_tag="IWL52_002357"
     CDS             2655..3455
                     /locus_tag="IWL52_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9778876.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3474..5342
                     /locus_tag="IWL52_002358"
     CDS             3474..5342
                     /locus_tag="IWL52_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9778877.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5335..5826
                     /locus_tag="IWL52_002359"
     CDS             5335..5826
                     /locus_tag="IWL52_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9778878.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5814..6368
                     /gene="srlA"
                     /locus_tag="IWL52_002360"
     CDS             5814..6368
                     /gene="srlA"
                     /locus_tag="IWL52_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9778879.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6386..>7380
                     /locus_tag="IWL52_002361"
     CDS             6386..>7380
                     /locus_tag="IWL52_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9778880.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2611 a         1187 c         1410 g         2172 t
ORIGIN      
        1 cagaatcaca tgccatatca tcccatattt cttcaagaaa ataaataagc gggaaaaagg
       61 gaaggaatag tagaacatcg atccagcacc atttttttct tcttttgaaa taacataaaa
      121 caataaacat gaacgtgcag ggaagtaaaa acaaagcttt actcataact aacaaaatac
      181 cagctccttt tatttttttc tcccccacat tcaaagagta acaatggtat gtacttaata
      241 atagaaaaat ggatcaactt gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa
      301 cttccagcca aaactgactg gaagtttatt tttttctgaa atattctaac acaaatatga
      361 aaaagatggc cgaagctaat aagccgattt cgtagcaaaa caatgagata ccagagtcgt
      421 aataataaaa tcctctaatc ttatggataa aatagatcat cggacaagca gataagagta
      481 agtatttgtt aggccttttg aataagtaaa agaaagcact ggtcattatg ataaatggta
      541 tccaaaacaa aagtttactt aagacaatca aatgatcaac tcccatattt tttaccaaga
      601 gagatcataa catctttatc gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa
      661 acatactcgt tcttatccaa atatataaaa atcgcccctc attcatcgag aggcgataag
      721 ttctaattta actcaatttt ttttgtttct tttggctgat aagctgtttt aggtaatacc
      781 aatttcagca caccgttttt aaaggttcca gtgatttttt cttcatcaac attaggaaga
      841 tagaatgaac gtttgtaaga gaccgaatgt ctttctttcc gtacatagtt gccatcttct
      901 ttatcttctg tatgggactc atgattggcg gagatcgtca aagtattatc tgaatattca
      961 actgtcgtgt cttctttgtc acagccaggc atatcagcag ttagtttgta ttctttttca
     1021 ttttcgacta aatctacttt tggataactt gaaacaccta aaaaatcatt gaatgctgga
     1081 ctaaacacgt cattgaaatc agggaacatg tcgcgaatgc taggtacatt tgccattttt
     1141 gatcatcctt tcaataagta ttttttattt atccctattt acgtaataac tctattttga
     1201 aaaaaatgca agtataatgg ttagctttgt gaatatttca tctaatacga tacaaaggct
     1261 atctttctaa tttgaaaaag ggatcattaa aataataaga agaaaatatg tcaaaaggta
     1321 aaaaagtaaa ttcttctatc caaatataga aaaattgaat ctagaaaacc gcccgacaat
     1381 aaatttcatt aactcagttg accgaaaatg aagtagatac caaaaattta tcaagagagc
     1441 aagtagagaa gtgagtatat tcataagaga aatagctgta acgaattatt aggttaatgc
     1501 aaatgcttat ttatactccg tataactttc tcagaaaaaa tgataccaat aatgacttgc
     1561 tagtactgta aatccgtata atatcatcta atcaaaatgg tgttccattt gcatctgtca
     1621 tcgtaatcga tccttcgggt gacactcgat aaaaaccaac gcttccagat cctccttggg
     1681 aacggatttg tttgctggaa gctgtgaaca aataatctcg atcaattttt tgataaaagc
     1741 taataacaat atcgtcactt tgtaattcgg aacggtgggc aataagaaat agaagtgctt
     1801 tgtcagagtt ttctaatgga agagaaggta cagtgctttc ttgcatttca gatgttgaag
     1861 aaatgctaaa cgactgaata cttgattggc tatgttgcga atctacatta ccagtagaag
     1921 tattagtaat ggaagtagta gaagtacttt tagttgaacc aacctcagtt gttgaaatgg
     1981 ctgctgaaga ttctttaaca gaattctttg ttttatatcc acaggcagaa aaaagtatta
     2041 cagttcccaa caagcctgcg taaatccctt tatgcatgat agctcctcct aaaatagatt
     2101 atctttattc agtatatcac ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct
     2161 tcccaataaa gggaaggttc atgaagataa taactttttt aataattata gctttctaat
     2221 atctttcatc aacttttcta gtaatcattg ggaaaaattt caagcagtag atcgcacaaa
     2281 tacctacgac gatataaatg atcttcgcaa aaatagttgt tgaaccacca gcaatcatag
     2341 ctactaaatc aagttcgaac aagcctacca acagccagtt caaccctccc acaatcaata
     2401 aagttaatgc aatagcatct aaagttttca ttagaattct cctttcttaa atgataaaac
     2461 aattatatat caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat
     2521 gcaaaaagat gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt
     2581 aaatttcatg tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa
     2641 ggagaggatc acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg
     2701 gaggttcctc aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag
     2761 tagcgaattt tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag
     2821 tcgatgtgtc ttctaaaacg gacatagaag aagggatata taaagtaatg aaacatttca
     2881 agacgatcga tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata
     2941 gaaatcatcc aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg
     3001 tcaatcaaaa aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg
     3061 gatcaggagt gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga
     3121 gctgttatgc tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt
     3181 taggaaagag gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt
     3241 tgagaacaca agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc
     3301 tgagaaacgg ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag
     3361 aagtggcgga tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca
     3421 cgactaatat ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc
     3481 tagtaaacag atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc
     3541 aggaagtgca aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt
     3601 tgaatgaaga aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg
     3661 aaatttatga ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg
     3721 atttcaattc ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg
     3781 atttcttacg tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca
     3841 aggatctaaa gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac
     3901 caaataaagg actaagaata ataggagatg agctagaatt acgactactt ttattttatt
     3961 ttgtttctcc ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa
     4021 tcaaacattt tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag
     4081 ttatcggtat ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc
     4141 gttacagtgg gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag
     4201 aaatctatga actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca
     4261 tcatcaacac aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg
     4321 accccatgat gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat
     4381 atcaagagat gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat
     4441 tgactgattt attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta
     4501 gcgaatgcat acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca
     4561 gctacctaac gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta
     4621 aaaaagtagc cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa
     4681 tcaaaaggat attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa
     4741 aagtagatct aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg
     4801 aagagacacc ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat
     4861 ggcaaaaagc aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa
     4921 tatggaaaga aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca
     4981 gaggctatta tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa
     5041 gcacatttgt taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca
     5101 ttgttttatc agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag
     5161 ttattttttt atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg
     5221 agctatacga tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag
     5281 caccgacaca agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat
     5341 gatatgtatc tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt
     5401 tggaagcaac taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta
     5461 gcgattggta aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac
     5521 gatcatgatc aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt
     5581 cattcattac ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca
     5641 gttcgagcgg aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta
     5701 cgaatagagg caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc
     5761 aataattttc ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata
     5821 tcataacaaa atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca
     5881 taagctggat gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc
     5941 tgattgcttt tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc
     6001 cgcttactcg ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt
     6061 catttacaat ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag
     6121 ctcaattttg tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg
     6181 tatggatggg gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta
     6241 tccgatactt gcttgtggga ctgctgatga actttattgg tggttggata actgatttta
     6301 ctactggatt tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag
     6361 ttgattagag aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg
     6421 cgggtatgga ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt
     6481 cacaggtgga ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga
     6541 gtctatcaat gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga
     6601 ctgtggaggg actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat
     6661 cgtttccaca ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc
     6721 agcagtaggg ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt
     6781 aaatactgca tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca
     6841 aaaagcagag cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc
     6901 tacattcaat caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt
     6961 tatggctttc gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt
     7021 tgctaacatt atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat
     7081 ttgttcactg ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat
     7141 tggaacattg atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc
     7201 ggcactattt gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt
     7261 ggaagaagca aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt
     7321 tcttaacggc atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDVT010000099         7333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDVT010000099 DACDVT010000000
VERSION     DACDVT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.101"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..569
                     /locus_tag="IWL52_002362"
                     /pseudo
     CDS             138..569
                     /locus_tag="IWL52_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            734..961
                     /locus_tag="IWL52_002363"
                     /pseudo
     CDS             734..961
                     /locus_tag="IWL52_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1087..2058
                     /locus_tag="IWL52_002364"
     CDS             1087..2058
                     /locus_tag="IWL52_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9778881.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2198..3511
                     /locus_tag="IWL52_002365"
     CDS             2198..3511
                     /locus_tag="IWL52_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9778882.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3554..4420
                     /locus_tag="IWL52_002366"
     CDS             3554..4420
                     /locus_tag="IWL52_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9778883.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4417..5265
                     /locus_tag="IWL52_002367"
     CDS             4417..5265
                     /locus_tag="IWL52_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9778884.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5279..6751
                     /locus_tag="IWL52_002368"
     CDS             5279..6751
                     /locus_tag="IWL52_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9778885.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6768..>7333
                     /locus_tag="IWL52_002369"
     CDS             6768..>7333
                     /locus_tag="IWL52_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9778886.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIE"
BASE COUNT         2284 a         1250 c         1579 g         2220 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaattgtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tagttttact gttatcatag
      121 catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt tgaatgaatg
      181 aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc tcggaacagt
      241 taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt agaaaaggca
      301 aactttatca aacttattac gacggtatgg aggaacagct caacgcatta ggattagtaa
      361 ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa caaattaaaa
      421 cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta attagtgatc
      481 acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg aatggaatac
      541 tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc taccgtgcat
      601 tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac acaagaatac
      661 taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt cgtttctatg
      721 ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca ttgacgactt
      781 agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg gtcctgttta
      841 ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg aaataaaatt
      901 gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg aatacgaaaa
      961 tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat tcctgctaat
     1021 ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag aggagttgtt
     1081 cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg tgtgggtacg
     1141 gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa agtaatggct
     1201 gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa aacgaatagt
     1261 agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc cgagtttgcc
     1321 tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg taatgcgaac
     1381 ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa agtagacgga
     1441 attataggta ttacctattc agacattgat aaatatgtat catcaaattt accatttgtc
     1501 agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa ttatttaggt
     1561 gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg ctatgttggg
     1621 ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta caatatttgt
     1681 gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag caatttagag
     1741 gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt taccatcaat
     1801 gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga agcacctaag
     1861 gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga ctatttacta
     1921 tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac actcatggcg
     1981 atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt tcacgctgga
     2041 gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag gttgacagaa
     2101 agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt aattgaaaaa
     2161 taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa tgttagcaat
     2221 tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta aggagaaaag
     2281 ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg atgaaacgcc
     2341 tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg agggtgaata
     2401 taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg aggataaagt
     2461 aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg gaccgaatac
     2521 tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga aagatgcgga
     2581 catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca aaatgtatgc
     2641 gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt tcaaagatgc
     2701 tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga cggaatttca
     2761 agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata tgcaaatggc
     2821 gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga atggcggcga
     2881 tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa aatccgttga
     2941 agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca gtcctattga
     3001 aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga caatggcaga
     3061 tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg tttcagatgc
     3121 aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg agaaaacaga
     3181 gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat ctagtcgtat
     3241 tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt taattaaaga
     3301 tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg gacatgcccg
     3361 cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag ttcaagatat
     3421 tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg ctgctgaaat
     3481 gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca atcaagtgtt
     3541 aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc cagctttgct
     3601 gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg cctttactga
     3661 ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt ttaagcgact
     3721 gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg ttgtttggat
     3781 tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca aaaatcgtaa
     3841 aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct tagtcgttat
     3901 ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata cgctatttgc
     3961 aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa tgtatacgct
     4021 tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt tagggggaat
     4081 gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt ctaagtttca
     4141 gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta ttttacttac
     4201 cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc aaggtggacc
     4261 aatgaattct actatgacaa tggtttatta catttaccaa actggattta cagaccgtat
     4321 ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt taattacaat
     4381 tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt tggaccgcta
     4441 atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt cccgatgctg
     4501 tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt agcttccttc
     4561 aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca agaggttatt
     4621 tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt aacctttgcc
     4681 tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt taatttttca
     4741 ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat ggagacggtt
     4801 ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg cttggcagtg
     4861 attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt ctttatcgcg
     4921 attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt taaaattttc
     4981 tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt cttatccttt
     5041 atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag cagcaaattt
     5101 tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat caatcaagtg
     5161 atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt tgcacaaaaa
     5221 tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg aatagtgaat
     5281 gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa cagtcaatca
     5341 agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg atccaaatgg
     5401 ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt acgacagcat
     5461 ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact gggagcattt
     5521 gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt ccggcagtgc
     5581 catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg tggaagggca
     5641 agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg aaaaatatgc
     5701 cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg aagatttccg
     5761 cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta ctaaaactgc
     5821 agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt gggagtttta
     5881 ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc cagacttgtt
     5941 tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc cacaaggcaa
     6001 tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt ggcaaacagg
     6061 caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt atgcacccca
     6121 gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa tgtggcagcg
     6181 taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct tgcctagaga
     6241 gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt atcaagtaat
     6301 tgatccgcag aaaaggattg aatgggcgaa gactgaagag aatcgcttct tactgaagga
     6361 cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg caacgaaact
     6421 agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg atgttgaacg
     6481 ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag ctgaaaagga
     6541 agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa ccatcgaaat
     6601 tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta tgagtacaac
     6661 tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg aagtaacctt
     6721 ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg atttatggtt
     6781 caatcgaagc aggcggcaca aaatttgtgt gtgccattgg cgatgaagaa atgacgatca
     6841 aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta gtcattgact
     6901 tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt gggcctatcg
     6961 atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa gtggcatggc
     7021 aaaattttga tttcatcggt accatgaaaa aagaatttcc gatcccgatt tcttggacaa
     7081 cagatgtcaa tgcggctgcc tatggtgagt atgtcttcgg cagtggcaaa ggattgagca
     7141 gtgtcgttta ctatacgatc ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct
     7201 ttatcgaagg gtttagtcat ccagaaatgg ggcatatgct cgtcgtccct catccaaaag
     7261 atgactttgc tggcagctgt ccatttcatg gcaactgttt agaagggatg gccgctggac
     7321 ccgccatcga aaa
//
LOCUS       DACDVT010000100         7288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDVT010000100 DACDVT010000000
VERSION     DACDVT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.102"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..370)
                     /locus_tag="IWL52_002370"
     CDS             complement(65..370)
                     /locus_tag="IWL52_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778887.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(367..528)
                     /locus_tag="IWL52_002371"
     CDS             complement(367..528)
                     /locus_tag="IWL52_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9778888.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(543..902)
                     /locus_tag="IWL52_002372"
     CDS             complement(543..902)
                     /locus_tag="IWL52_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9778889.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(899..1750)
                     /locus_tag="IWL52_002373"
     CDS             complement(899..1750)
                     /locus_tag="IWL52_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9778890.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1767..2597)
                     /locus_tag="IWL52_002374"
     CDS             complement(1767..2597)
                     /locus_tag="IWL52_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9778891.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2600..3286)
                     /locus_tag="IWL52_002375"
     CDS             complement(2600..3286)
                     /locus_tag="IWL52_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778892.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3292..3531)
                     /locus_tag="IWL52_002376"
     CDS             complement(3292..3531)
                     /locus_tag="IWL52_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778893.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(3857..4045)
                     /locus_tag="IWL52_002377"
     CDS             complement(3857..4045)
                     /locus_tag="IWL52_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778894.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(4066..4350)
                     /locus_tag="IWL52_002378"
     CDS             complement(4066..4350)
                     /locus_tag="IWL52_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778895.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(4352..4546)
                     /locus_tag="IWL52_002379"
     CDS             complement(4352..4546)
                     /locus_tag="IWL52_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778896.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            complement(4543..5319)
                     /locus_tag="IWL52_002380"
     CDS             complement(4543..5319)
                     /locus_tag="IWL52_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9778897.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(5446..5667)
                     /locus_tag="IWL52_002381"
     CDS             complement(5446..5667)
                     /locus_tag="IWL52_002381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778898.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            5968..6312
                     /locus_tag="IWL52_002382"
     CDS             5968..6312
                     /locus_tag="IWL52_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778899.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            6348..6743
                     /locus_tag="IWL52_002383"
     CDS             6348..6743
                     /locus_tag="IWL52_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9778900.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            6796..7107
                     /locus_tag="IWL52_002384"
     CDS             6796..7107
                     /locus_tag="IWL52_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778901.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            7119..>7288
                     /locus_tag="IWL52_002385"
     CDS             7119..>7288
                     /locus_tag="IWL52_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9778902.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELEL"
BASE COUNT         2052 a         1400 c         1178 g         2658 t
ORIGIN      
        1 tctttctgaa tcttcatcat gattttcttg aggatgatgt cactgtattg tgtaatgaga
       61 tccattatct ctcctccaca tacctaaact gtcgtccttt tgaatcaatc cataagctcc
      121 tagctctatc ccagataatg tttttgctta atccagtaat ttcagataac tgttcagctg
      181 tacctgttac tagaattcga tcaccatgcc agattgcaat ttttctcggc gttttccgtt
      241 taggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact atttctttgt
      301 cttcctgcca agattctgac ttggttaatt cagcaattcg tttcattgtc gctttcttat
      361 ccacgctcat tcctccaatc tacgaatttc ccttcttaag ttctctatgt gcaaatcgat
      421 tgcctttctc gccgtttcat tgaccatcac tgcctttgtt cgttctagat cgtcaatttc
      481 acgctgaatg tttcgaatac gcatttgaat cacttcttct gttgtcatga tggaccacct
      541 cgttaaaaac gctcttcctt gaacgtattc cgatattttt tagctaatat caacggcgct
      601 tgatattgat gacagaacaa ctttgccttg atcttaaagt cttttgtctg cattcctttg
      661 acatctacga ctttgacaag tttgccgttt ttataaaatg tgaagtcggg aatatactcg
      721 atcttgcgat acttctttcc gtctagttca aatttcggca tcagctcaaa tctttcctga
      781 agttttactt tccagccatt tgcttcagct tgccacaagg ctagatcgta atactctgct
      841 tctgcgatag aatcaaactt gatacctcga tgaacagttt ttttattacg gtatttattc
      901 atgcgatact acctttcact ggttttatgc gcttgtctgc tgtttgttgg aatttcagcg
      961 cataaccttc tgaattctta aatatcctag aaacaattct ttcaccgtag gcttctctta
     1021 gttcaggacc agataagttt gttgtgatga tcgttgcctt gttctgtctg gcttctaaga
     1081 gcgtgtttaa cgtgttgttt gtaaactgcc tactatttga taccccacta cctaattcag
     1141 ctccaatatc gtcaaaaacc accaaatcag ttgttttaat atcggctata agcgatcctt
     1201 caatttcttt tctcagttca gtattgttat aagaaaactt tatttgctct aataattctt
     1261 gatagcttat aaaaagtatt ttcttgtcat aatttgagcg ctcaagtatt tcccaagctg
     1321 tggccattga taagtggctt tttccgcttc ctgatttccc tgatagaatg aaatgtgcag
     1381 gatggttcag cagaacctca ttcgtatagc tttttgcttt ttctaaagcg atttttgttt
     1441 cttgatccac tacgtgataa ttttccattt tgcatttaaa caaagtttta tctgttaata
     1501 ccgaaccatt ttgaaaaaaa ctcaacgctc gtgcttttaa gctatcgtta tatatccgtt
     1561 cggtctgtat atcctctttc acacgtaacg ctttataacc acaactcatg catgttggtt
     1621 tacaacgttc tgaaccatcc ttatttttag ctcgccaact atacaaaggt tcgctacatt
     1681 ctggacattt tccgctttgc actaatactc ttcttattag tttctccata gcatttgcta
     1741 ggctttccat gtgatgcatc tcctttttaa attggcaagt cgtcatattc actaggattg
     1801 ctgtactgta gtttttgact ttgcttttta tgattcttct tgtctgcttt gatttcgaat
     1861 ttgagcttct caaatttttc tctcaatttc ttagcacttc taatatttcc aaaccaaaat
     1921 tcatttgtag gtagccaatt gatcacatac tcaatcgctt ctatagatgc tttatctctt
     1981 tcttccatca acctgattgt gtctgcccat ttttcgatat ctactttgtt catttctttt
     2041 ggaaaatctt cagttaaatt actttgcaac tttttagcaa ggcgtaagtg ttcgtcagaa
     2101 tacttacctt tcttttcttc tttatctata tctttatctt cttctatatc tttatctgta
     2161 ccgtcacgtg acgtcacgct aacgtcattt tccaatttga gacgttcctg tctctttctt
     2221 tccctgtatt tacggtttct ttcagcattt tttagcctta ctttatccat accctcgata
     2281 ttttgatgtt tttcccaatt actgatggca attagtccat cactgcttag atcaatcatg
     2341 ttgaaatttg ccaatgtagt tagcgctaag cgaaccgtat ttacgttttt gccaaacaat
     2401 gtagcaagca tttcttcggt ataaggcatg ttcctctgga tatatatcag accatcgtcg
     2461 ttagtctttc ctgctaaaac tagtaatcga atccatataa cgatgatggc atccgactca
     2521 ggaacagctt ggattaatcg tattttttca tcgtcaaaca tagtagtttt aagtttgatc
     2581 caacttatct cagccaaatt tatcctccta tccttaactt tttaattgtt tcctggttta
     2641 acttgatccc tttgatttga tatttatttt tgaaattgat cacacctatt ttgtgtttct
     2701 ctgtgtgatg gattctgcag agtgctgcaa atgtgtactc tgaatgatca acttctttgc
     2761 gctttcgtct tcccagcgct ttgtcaaagt gatcgatatc agctcctgtt ttgccacaga
     2821 tgcagcagac tctttttgta atgcatttgt agaagtaata ctcttgattc gctggtaaaa
     2881 tctcatatcc ttctttgaaa ggaatatgat gttcaaagat gaaatctagg atgatattcg
     2941 ctaagatatt ggcatcactc acggttgtat tcgattcgtc tttgaggctt atttcgcgcc
     3001 ctgtgacacc ttcaaaacgg aagtagaaga attccttcca gaagtccgtt ggcatgcctg
     3061 tatcgatgaa aatatcgcct atgagcgcat agatgaagtt tctttgctgt actgtgaacc
     3121 gtcgtggatc aataaagcga atttcaatga ctcgatcgcc atcatatccg tcatacatcg
     3181 ttttcagccg ttcgatattt acttcttcgt tgattgttgc accaatgtcg ttacctttaa
     3241 atttctttag taccgccgag tatgaatcta ttagtggttt aaacactcat atcacttctc
     3301 ttttgtttct tctctgtact gatcttcaag ccaattaacg cctcgtttta gaatgcccaa
     3361 gtctctcttg gtccatttac tgtcatcagc ggttatggaa gctgcatcag tcaatgcaac
     3421 aattgcttca tcaattgatt tttcgtactt gttagcaacc agttgtaaat tcgatttgcc
     3481 ccaatcagga tcggccaaat attgatctag agtggaaagt tcattttcca tatgttaaaa
     3541 tctccttagt tatgattttt tgagtgactc attgctttgg tcggcggagt cactttttta
     3601 tttgttgcca tgcttttttc ttttcgatat gttgcttgct taaaataata gaacggctat
     3661 ttgcccacca attatcagca atcactttac cgatttttag cgcttcttct cgtgccatag
     3721 ttgctccttt cttttgagtc aagcagattg attaaaacca tcaatgctgc gaacaagctt
     3781 cctccgataa tactttggtg cgctacaatc actaataacc ccaaaatgaa tcctataaaa
     3841 agtgtgtctg tcttcttcat aatctaatct ccctattttt tatttctagc attctcaaat
     3901 cctcaagttc agaagcaatt agttcagctt gtctatctga tagctcatcg gattttctaa
     3961 gcgctgcacg atcatcttgt aattgttttc tacgctgttt aatcagacct agaatttgat
     4021 gttcttgttg caatgtgtag gacataaaat cattctcctt tgcctttaga actcaaagtt
     4081 ttctttcaaa aatctttgga gttccgatcg ttcaattctg atgtctaact tgctccactg
     4141 ctgtgttttt aagcctaggc ttatccaatg tgttaatttg tcatcaccaa tgcctaaaac
     4201 tttttttacc tctgatttgt ttggatatgg aggaagctct actgatttgt tcataaggtg
     4261 taatcgttcg tccaatgaat taagaacagc attcgtaatc tgtgtagtta attcggaaac
     4321 tactaaatta tctggaattg ttatttgcat gttatttctc cttctctaac tctgctagtg
     4381 ctgcctcaat tggcttgatt tgtttatctg gttttctacg cccattcata atgtccgaca
     4441 tatatggtct agaaattccg atagtatcag ctaaccagtt ctgagacttt ccgtattttg
     4501 ctaattgaat tctgactttc aaaataaatt cttgtgacat cgtcattcct cctctaataa
     4561 atcgatttct ggaatatatc cttctttttt cagcgactca taaatgaaca gacgcccttt
     4621 ttgagtccat tttgtattca tcacaacttt tgttccgcca tcagatttcg gaatctcagt
     4681 tgtatgagat tttgtgtaac cctgtctcat gtgttttttg cataataacc attggttgcc
     4741 tacttttttc tgaataccta gcttatgaag tagtttgttc atctgttgtg gagacatccc
     4801 ataatcagcc gcgatttgac taatagttac tgaatctgta gaagataaga tactatccaa
     4861 atacgaaatt ttgggttcgt attctgcaat tttttgttct gcgattagtc ttccagtacg
     4921 ttcttctttc aacttagttg ctaattggat gattgtatct ggattaagca aagcttcttc
     4981 tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca cttcgctagt
     5041 cacccaacgt ttaaatttct tagcatttgg ttgtttgctt cctaaaatta aggagtacaa
     5101 acctgattcg ttaataatag ttaaccctct aggcgattca aaagtaccgt tttggtagtt
     5161 ttgccgatct tctacatcga cgtgtctgtt aatatctcta ctaccatttt ggtaccctag
     5221 aactttagcc acatcttttc caacaaaaaa tggttcatca tgtattgtta ctgttcttac
     5281 ttcatgttgt tcaaagttaa aaatttgcgg tgtgttcatt tttgctcatt cctttctttg
     5341 atataatttt gaatagaaag cgaggtgaaa atagtatgga agaatttaat atggatgtcg
     5401 acgccttatt caaacaaacc gtattcaaga ctgttaataa agaattcaaa atagacttcc
     5461 aaaatgacga atcttttcca acagaaattg aattattcga agaactttct cagagcgttt
     5521 ctgagtcttt ttctcgtcag ttgcaaaaga atttttttga tgctcttgtg gatgaatttc
     5581 atcaacaaca gcactaaact tttggtcttt aaaatttaaa gttattaaag ctccttttgc
     5641 ggaaggagtt tttttatttt gtttcatgat gtttcctcct tttctttaaa tatgtaagct
     5701 aataaaatta gctaattttt attgacattt ctatccaact gtcttatatt tatatacagt
     5761 taaataagcc tagaaaaacc ctaaaaatta acattcttag tttgccgacc tcgaattcgt
     5821 ttattttatt aggtgtattt cttgttgctt gttagcttat taaattagct tacgagttaa
     5881 atataagaca attggataga tttgtcaact acctttctat ctttttgtat aaattattat
     5941 cgtacgctct aggaaggttg atttgacatg cttttcgaaa gggttaaaga gctctgtaaa
     6001 aaaagaggta tttctatttc tgaattagaa aatagaatag gatttggaaa aaactctatt
     6061 tataaatgga agacacaaag tcctaaaata gaaactttgc aaaaagttgc tgactacttc
     6121 catgtatcaa cagattattt gctaggacgc acagatgatc ctaacgcggg agttgcacca
     6181 gaggaaagaa aactaaccgt ggaagaagct ttagcatctg ttatgagtag cgacggaaaa
     6241 ccgctcaccg ataatgatag ggaaatacta tcagctatga ttgaagcata tttagagaaa
     6301 aaagataact aaataagtag gtgagtcatt tggacagtca aattgaaatg ataattaatg
     6361 aactcggcgt taaggtagaa gagcgtgaaa accttgatgc cgatggccat tatgttgctt
     6421 gtatgaatac catagtaata aaagctaatt tatctaagta tagaagacaa agaaccttat
     6481 tacatgaatt aggacacgct tctaaacatc atgataatta ttttttatat aacttagcat
     6541 tctctctcca ttcaaaaatg gaatatgagg ctgatcgctt catgattgaa aaattattag
     6601 atagatatat tgcaaagtct gaattagaac cacacaatat caattacatg aaatttatag
     6661 aagataacaa tttaagcgtt cgcttcgaac cacttgtgaa agaattatta aaagctcgca
     6721 tctattgtta tgcagctctc taaaattttt taagcaaaaa agaacatacg ttcaaaaata
     6781 gaaaggtgaa caaaaatgat atatacagaa ttcaaagaat ggttagaaaa aagcacaacc
     6841 ggatacgaaa catttatcat caaagctact aattatcaaa ttgaaaaaaa caaaaataga
     6901 ccccaaaaaa aacgctggga tgataagaaa atagataaag ctgtattaga aatgtggaaa
     6961 caagtcgtga ctaacttgta tcaaacaatt cgtaaagaaa aaggagttcc attaattaac
     7021 gggaaggaaa tatggcttga atttatagag gaacaaggac tgatcgaatt tttcaatgat
     7081 agcatggcag aattagaatt tgaatagggg taatattgat ggcaatgata aaacaatata
     7141 aaaagaaaaa tggcgaaaaa gcatggtact ttaaaactta tctcggtatt gatccgctaa
     7201 ctggaaagaa aaaatatact actaaacgag gatttagaac acaaaaagaa gcaaaaacag
     7261 cactttctag gttagaacta gaattaca
//
LOCUS       DACDVT010000101         6989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDVT010000101 DACDVT010000000
VERSION     DACDVT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.103"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..152
                     /locus_tag="IWL52_002386"
     CDS             18..152
                     /locus_tag="IWL52_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAP9778903.1"
                     /translation="MKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK
                     "
     gene            398..994
                     /locus_tag="IWL52_002387"
     CDS             398..994
                     /locus_tag="IWL52_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9778904.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1007..1906
                     /locus_tag="IWL52_002388"
     CDS             1007..1906
                     /locus_tag="IWL52_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9778905.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            1909..2393
                     /gene="ssb"
                     /locus_tag="IWL52_002389"
                     /pseudo
     CDS             1909..2393
                     /gene="ssb"
                     /locus_tag="IWL52_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2415..2672
                     /locus_tag="IWL52_002390"
     CDS             2415..2672
                     /locus_tag="IWL52_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778906.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2831..2965)
                     /locus_tag="IWL52_002391"
     CDS             complement(2831..2965)
                     /locus_tag="IWL52_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9778907.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            3108..3368
                     /locus_tag="IWL52_002392"
     CDS             3108..3368
                     /locus_tag="IWL52_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778908.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3817..4023
                     /locus_tag="IWL52_002393"
     CDS             3817..4023
                     /locus_tag="IWL52_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9778909.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            4023..4274
                     /locus_tag="IWL52_002394"
     CDS             4023..4274
                     /locus_tag="IWL52_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778910.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            4290..4727
                     /locus_tag="IWL52_002395"
     CDS             4290..4727
                     /locus_tag="IWL52_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778911.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5408..5803
                     /locus_tag="IWL52_002396"
     CDS             5408..5803
                     /locus_tag="IWL52_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778912.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEISVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGIRITEEKVVWVNNHTELGALAFEGTVLDNISIVFEEE
                     SFTLECDKLTRYIDPKFYEDKNLAWELAL"
     gene            5785..6051
                     /locus_tag="IWL52_002397"
     CDS             5785..6051
                     /locus_tag="IWL52_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778913.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATLESRVQKKEVDLTHTYL"
     gene            6061..6294
                     /locus_tag="IWL52_002398"
     CDS             6061..6294
                     /locus_tag="IWL52_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAP9778914.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            6634..6816
                     /locus_tag="IWL52_002399"
     CDS             6634..6816
                     /locus_tag="IWL52_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9778915.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
     gene            6806..>6989
                     /locus_tag="IWL52_002400"
     CDS             6806..>6989
                     /locus_tag="IWL52_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778916.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEE"
BASE COUNT         2539 a         1098 c         1426 g         1926 t
ORIGIN      
        1 gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa gggttcattt gaagcaacga
       61 ttgttttaaa agaagacttc actacaggct ttgaattttc tgaaaagaaa aaaacgaact
      121 tcaagaaaag aacacgccgc actgcaaaat aatggagggt taatcttatg cgtaatacca
      181 cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa
      241 tgcgtgccag caaacgaaga ctgcagcaat tgaagaacca gcagaggatt caaaaagaaa
      301 tcgaattaga tcgataaaat cttcctaact attgtatacg tataaactta tgttatactg
      361 tttttataag tttatacgta tacaaaggag ggagaaaatg acaaaagcaa tggtatacaa
      421 gctgctagaa gaatttaatg gaactttggc tttgaaggat gccaaaaaag cgggcatttc
      481 tcctgtaacg atcaaacgta tggtagatcg aggggagctg gatcgagagt atccaggttt
      541 tttcacctta cctggtcaat ttcctgatga attgtttatg gcgcaaaaaa aatatgaacg
      601 cggcattatt tcacatatta ccgcgttaga tttgtatgat ttaacagata tgatccctag
      661 acagatcgat ctgactgtac cttacggtta tcacgtttct gaaaaaggat tgaaggaatt
      721 cgcagttgag ctacactata caaaagcgga atggtatgaa ttaggtaaga ttgaaataaa
      781 aagtcgctat gggaatccta ttatagctta tgatacagaa agaactttat gtgacatttg
      841 gaatccctgg tataccgttg aggatgaaat caaagtaaaa gcaatcaaaa actatatgga
      901 gtcggacaga aaaaatctta gaaaattgaa tgagtatcga cgaattttac caacagacaa
      961 aacgatgcgt tcttatatta tggctttaaa ttaaaggaga agatacatga cacctacgca
     1021 gttaaaagca aaagtgaaga atatttctag agaaaaagaa gttgatccgc agcttgtttt
     1081 aagacatttt atgatggaaa aattcttgga aaaaatctca gagtctcctt atcgtgaaaa
     1141 ttttgtgtta aaaggcggct tcttgattgg ctcaaagtat ggcattgaaa atcgcacaac
     1201 caaagatatt gatacgactt tacgggaaat gaaagtaacc aaagaaacat taacaacggt
     1261 tctaaacgaa attttttcaa cgccgacgaa agaaggcatt caatttgaga ttcaaggaat
     1321 gaaagaaacg agagaagccg attactatcc aggatttagc ttacgtgtgc tggcacattt
     1381 ggaaaacatg cggccagatt ttaaggtaga tgtgactaca ggtgactcga tctatcctgc
     1441 aacgatcact cacagtcata aattgatgtt tgaggatcga acgattcctt tggaatccta
     1501 tcctacagaa caaattattg ctgaaaaatt aagtgcaacc tttgattttt taacagataa
     1561 ttcgagagga aaagattttt atgatctcta tactattcct aaaatggaga agatcgatga
     1621 gaagaccttg aaagatagtg tgagaaatac atttacacgt cgaggaaaaa ctgatccgtt
     1681 aaagatatac tatgaaaagg atatggatgt actgaaaaat agtcacgagc taaaagatac
     1741 ctggaagaaa tatcaaaaaa gcaatcctta tgctgcatcg atcacgtacg atgagacaat
     1801 ggattcgatt agtaatttga tggaaaaagt gattcacgtc gaagaaaaag agcaaaaact
     1861 agcttatcaa agacaccaac agatgaatag agaaatggag agataaagat gattaataat
     1921 gtgacattag taggacgctt aaccaaagat cctgatttac gttataccgc aagtggtact
     1981 gcagtagcga cttttaccct agccgtcaac cgaaatttca cgaatcaaaa cggcaatcga
     2041 gaagccgatt ttattaactg cgtgatttgg cgcaaaccag cagaaacaat ggcgacatta
     2101 gctaaaaaag gtagcttgat tggtgtagtt gggcggattc aaacgcggac ttacgacaac
     2161 cagcaaggac aacgtgtata tgtgactgaa gttgtagcag ataactttca actattagaa
     2221 aataaagcag cgacagagag ccgtgcgcac gctgatcaat cgtccaccag tccaagcacc
     2281 gccacctttg aacaacggat acagcgacgc ctaacaacaa tggattgaac gcttcacaga
     2341 atccatttgg tggacaatca atcgatatta gcgatgatga tctaccgttt tagtttgaga
     2401 aagaaggagt aagcatggca aaaaaaatta gtctgacgat ggatctattg atacccgtaa
     2461 ttgcatttat agacgggatt tctttgggcg tcactgaaaa tcctttggta aatagaattg
     2521 cgaaaggatc actatttctt gtattcgtgt atcttgtctg ttcttatctt ttaaaaagag
     2581 caggaaagga taattccttt tggcgattga ctaaagtcac agtagcagcg acagctgtta
     2641 tacttacggc tattctaccg ctttatatgt agagttaacg ggaaatcgtt gaacttcttg
     2701 cgaaatattg ctataatgaa catgaaaaag aggagttgcg ctaacaaccc ctcattaaca
     2761 gcaccgatta aagaacggtg gcagaatatt agttatttgg aaataaccgc tgacctgcta
     2821 aagtagacgg ttatttcttt ttgtcatttt taagcatatt tacaatcaaa ctaatcaaag
     2881 caatggtaaa cataccaaaa ccaagaatgg tttgaattgt ctcatatgcg gacaaaaggc
     2941 ttactccttt tatagatttt agcacttaca ttcataagca ccaccacctt tccagaagtc
     3001 gccaccgtct tcaaacttta ctgtcttgac tattatagca tatctaaaat cgaataaaaa
     3061 ttctcaattt taactgtttt tgctataatt ttagcgaggt gatcgtgatg aacaaatatg
     3121 ttggtttgct tgataaaatc cgagtaataa aaacacaacc attattggtt cgctttactt
     3181 tacaaacaat tcatgaatcg attaattgtg tagttgcaga tattgaaatt atcgataaat
     3241 tgttaattat ggatgatgga aaatacaata ttgccgtcac aggtcatttc aataaacgga
     3301 atcaattagt cattgaatcg atgcaaatta gaaaccctga tcattttacg cgatcaatgg
     3361 gcatataaat aaaagaagag ctgcaatcct aatgtaggac tgcagctctt tttgttttac
     3421 atagaaacag gaagaaaggg aaaggcctgt ttaggaaggc acaagtgcct tctcaattga
     3481 aaaacctttc tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgttgc
     3541 acgttcacta agataagggg tcttgctttg taagacaaaa taggaaaagc ttcctctcac
     3601 caaaaggttc gttcctgttc gctatctctt tattgatttg ccagaccggc aacaagttgc
     3661 cttttatcac caaaaagcga caaggacaag ggtaaatggc acgctgcgcg cccttgtcct
     3721 tgtcgctttt cgttgattca ttctggccaa caaatcaata aagaaagaac taacgaaagg
     3781 aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc gaaagaaatt tgattatttg
     3841 ggtgttccct tcagcgaatt attcccaaac tatcatgcac cacatactgt cgaatgcgat
     3901 tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt atcaatgtcc aacatgcggg
     3961 aaaaaatata ccttgtctta tggcgattat attttgattg aggagaaagg aaagaaacgc
     4021 tgatgaaaac acaacacaaa ttattgactg taaaagaagt cgttcaaatt ttggtggaca
     4081 aagaagtcat ttctgaaaaa gtagcagaag ctttacttat tccgcggtac tggacgatag
     4141 aaaccccttt agtagaggat gcaccgacta tgctatttga agacctagtg aagattgcag
     4201 gatgtgaact gcattatcaa gaaaacttgg aaacttatga aaacgaatat tggttaacca
     4261 aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa ttactatgac gaatttgtca
     4321 aattaccttt ggataaaatg gcacaaaaaa tggaggatat gacgttccta tacaacgaaa
     4381 cacgtgttcc aaaaaaacat tataaagaaa aattgtcggt agctgtggaa gaaatgattg
     4441 aatcaggtgt cgaaatgaat cttattgcga cgtactatcg aacactggaa gaactgaaaa
     4501 agcaaaatgc aaaatggttt ttccaagcct tactatgctt tgaaataggt gtcaaaccaa
     4561 gcactattaa accttctgaa taccaggctt tagaactaac gtatgcgaaa tttatcgaaa
     4621 cgaaaaaagc caagactgtt tcaagtgaat ggttagatta ttttgaaaat atcaataagt
     4681 atggcgcata ttacaccatg aaaaaggaag acaacgaaaa tgaataaggt gatttatcaa
     4741 ctaacaacgg attccatatc aacgatttga aagaaaaaaa ggtcaataat catttatacc
     4801 gaaaagtgag aatgtgtgga ataaggacaa aaaaagccta gtaactaagg gaatggttct
     4861 tatttcgcac caaccgcccc cgccgcttag gtggtgcgaa ataaacgggc aaagcccgat
     4921 aacttaagag aaagaaggtg atccatttga aaaagattat tcccttattg ttagtactgt
     4981 ttgttggcga aatacttatg cgagatagtg cagctgtaca gttaaacctc gctgtatttt
     5041 tggtaatact ggtgttttgt ttgatctttt ggcggtttgt attagtcctc gtgagtctac
     5101 tattcaagat gattgcagcc atatttttca tggggttatt tcgatgaaag gagagagtac
     5161 caatgccaat tgcaatagga aataaacggt tacccgtgac attagatgaa aagagacaaa
     5221 aagaattgca gcaactaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg
     5281 cgttagattt attgattgcc aagaaaaagc aggatttgag gtaccagcac taagaaagtg
     5341 acgtcacttt ttatactaaa acctaaaagt gatatcactt ttagacaatg gaaaaggtgg
     5401 aaataagatg aagaaaaaac aaactaaact atcgttagca gatattttaa cacagttaac
     5461 cgcaactgaa gatggtgttg tagaatttga acgatctgaa atttcggtag tagatgatcg
     5521 ttactttaaa atgccttact tttttgacca agcaaaggtc atttgccttt gtggctacga
     5581 tggggtacgt gattactttg gaattagaat tactgaagaa aaagtcgttt gggtaaacaa
     5641 tcatacagaa ttaggtgcgc tagcctttga aggaactgta ttggacaata tctctattgt
     5701 attcgaggaa gaaagcttta cgcttgaatg cgataagtta acccgatata tcgatccaaa
     5761 attctatgag gataaaaacc tggcatggga actggcttta tagacagagt gctagtgcaa
     5821 gtaaaggaag aaaggaagat caccatgaaa tacaatttat tagaccataa gcgaatgtgg
     5881 gtagagaaat tagaggaaga cttagaacag ttggaattgt taggtcataa taagacttca
     5941 acggtgtata aagaaggact aaaacgaacg aaaaaagcac aaaaagaact agcaacactg
     6001 gaaagtaggg ttcagaaaaa agaagtagac ttaacacaca cttaccttta ggagtagaaa
     6061 atggcagaca agagaaaaga aaagattcaa aaattactgg cgcttgcggc ggatgtaaat
     6121 gatgaggaat cagtgagcgc attagccaaa gcccagcaat tgatgatgga ctatgatctt
     6181 actgaagatg agattttcaa ttatcatgag cagcaacgga tagaagcagt agaaacgcac
     6241 gtgatctatc gtggacgtcc gcaaaaatgg ctgtatcgtt tatttagaaa ttaagataaa
     6301 aaaggctagt taacaggttt gttgactggc cttttttgat atgtttgtac atgttttatt
     6361 gtgttttaat ttttctcttt tttatagcaa gctcctttta taataaatat tccgaaaagt
     6421 gaagaccatt ttttatttat taagactatt attagtgaga taaaatttga ttgatcttac
     6481 agaatattgg tgacgattat gacactaaag atactattta tgaaaatttt gcaaaaatgt
     6541 tctgaaaatt acatataatt gttttataaa aaagggaggc aattatatga agaaaaaatt
     6601 tgttagtatt ttttgatttt aggaattgtt ttattgagtg tatctacttt aggaattaca
     6661 gtagatgctg caacttatta tggaaatggt gtttattgta atactcaaaa atgttgggta
     6721 gattggagta gagctcgctc tgagattgtt gatagaggag taaaagctta cgttaatggt
     6781 tttacaaaag tgctaggtgg agtcggtgga agataaaaaa gaaaaacttt ttgacttaat
     6841 tgcgaatgca tataattcaa agtcatataa taatgtagaa gtaaaagaag gatttcttca
     6901 agatgctaag aaaatacgag aggataaacc atatcctgaa attattatat cagtgtacaa
     6961 acatttatct aaattaaaag ctgaggaac
//
LOCUS       DACDVT010000102         6568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDVT010000102 DACDVT010000000
VERSION     DACDVT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.104"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            71..757
                     /locus_tag="IWL52_002401"
     CDS             71..757
                     /locus_tag="IWL52_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9778917.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            757..1917
                     /locus_tag="IWL52_002402"
     CDS             757..1917
                     /locus_tag="IWL52_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9778918.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            1927..2802
                     /locus_tag="IWL52_002403"
     CDS             1927..2802
                     /locus_tag="IWL52_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778919.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            2799..3110
                     /locus_tag="IWL52_002404"
     CDS             2799..3110
                     /locus_tag="IWL52_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778920.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            3100..3453
                     /locus_tag="IWL52_002405"
     CDS             3100..3453
                     /locus_tag="IWL52_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9778921.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            3443..3844
                     /locus_tag="IWL52_002406"
     CDS             3443..3844
                     /locus_tag="IWL52_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778922.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            3837..4241
                     /locus_tag="IWL52_002407"
     CDS             3837..4241
                     /locus_tag="IWL52_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778923.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            4253..4861
                     /locus_tag="IWL52_002408"
     CDS             4253..4861
                     /locus_tag="IWL52_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9778924.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            4881..5243
                     /locus_tag="IWL52_002409"
     CDS             4881..5243
                     /locus_tag="IWL52_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778925.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            5246..5428
                     /locus_tag="IWL52_002410"
     CDS             5246..5428
                     /locus_tag="IWL52_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778926.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            5445..>6568
                     /locus_tag="IWL52_002411"
     CDS             5445..>6568
                     /locus_tag="IWL52_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9778927.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGL"
BASE COUNT         2414 a          985 c         1365 g         1804 t
ORIGIN      
        1 gataaggcga tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga
       61 ggtgaggcga atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc
      121 gcaaattttt attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa
      181 gggaatcaaa aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa
      241 ttcaaacggt ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa
      301 agcaaaagtt aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat
      361 ggctggcgat actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac
      421 atacggagaa ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga
      481 taaatcggta aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga
      541 acttcttgaa aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga
      601 tgaaattatc gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga
      661 agaaattttg aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc
      721 attaaataaa acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt
      781 gtaacagctg caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt
      841 agcgcagcta tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca
      901 gcaaaaaatc aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa
      961 gaaacaaaat tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta
     1021 gtgccagcta ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg
     1081 caaaaaatta cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc
     1141 aatccagcat ggtggggtaa actttgcgaa gctgttaaaa aagttttaga taatggcttt
     1201 gatgtaatta acatgaagca gttcaagcta tcaggttata ttcctgtatg taaggcaatg
     1261 cttgatttag gtccagtatg gttagatcgt tatgtccgta ctgttttagt agaatcattg
     1321 agaattgcat tagaacaagc aattgttgat ggtactggta aagatatgcc agtcggaatg
     1381 atgcgtgaca tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc agaacctatt
     1441 acagctttag atgctgtaac tatgggcggt ttgatggcgc gactatcaaa attcaatatc
     1501 gaaggtgtga atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg
     1561 aatccaactg attactggtc taaagtattc ccagctaaga ctgtactaac tgctaatgga
     1621 gaatacgtac aagtattgcc agtaccagtt tcagatttgc agtcaacggc tgtgccagaa
     1681 ggaaaagcag ttattggggt agcctcagat tacttcatgg gtgtaggatc tacactaaaa
     1741 attgaagctt cagatgaata ccattttgtt gaagacgaac gcatttatct agctaaacaa
     1801 tatgcaaacg gtcaacctaa acgtaacgat agtttcattg tattagatat tagcgctttg
     1861 ggaactacta ctacaactac aaaaccaaca accacaacaa ctacaacaca agcgtaggtg
     1921 atcagaatga agtatattct ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa
     1981 gtttgtttaa ctaatctgaa gaaactagga atcaaagata tcgtattgct tttcagcaga
     2041 cacgatgatc agattcctat tttttttgag aaggaatatg gtgttgaagt tcatgtgtac
     2101 gatgatctgc gggacgacaa agagtatatt ccttcgatta aaccatattt atggtggaaa
     2161 tatttagaag aagatcattc gcgtgaggac gaccgatatt tctatatcga ttcggatgtc
     2221 attttcaata aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt
     2281 agcgactgct gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat
     2341 attctaaaag atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac
     2401 actaattcag gaggcgcaca gtgggttatt aaccgcccta aagcgaatta ttggaaaaag
     2461 gtttatctgg attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatatacaa
     2521 atctggacag ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct
     2581 aaagttcacg aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa
     2641 gtaaaaatct tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg
     2701 agatacgtga cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc
     2761 tcttacgcat atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg
     2821 aagaattgct aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt
     2881 caattaaaaa gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac
     2941 tttctttttc tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact
     3001 ggaatcgtgc tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc
     3061 aacattatgc gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc
     3121 gaagaaacat tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa
     3181 ctaggaaaaa agattggtgt agaagataca gaaatcactt ctttaaaatt tagaaatctt
     3241 tccatgagag atagtgatat aacagctatg gatgcgatgg gatcgaaatt aactaagaaa
     3301 gtaaagactc catttcatcc aatcgccaag aaatttaata aagatcaata ttttatcgta
     3361 atcgatagta tgcgttacaa cgttatctat gccgattacg ataattttta tatctatttt
     3421 tatcttgaaa gtgtgggtga atatggtgat tgataattct aaagaaaaag aacgtttaaa
     3481 taagcaaatt tctgctatca aaacttcctt agaagagcat tttggcctca aactctttca
     3541 agactccgta ggcgaggatg agctacctga tgattttaat tacttcattc tcgaaacagg
     3601 agaaatagaa atgatcactg agccaaaata tagcgtgggt caaaatctat atctaacttt
     3661 ctattcagaa aatagagaag atttaacagg agactcacta gatattattt cattgattca
     3721 aaatcgttcg attcgttttc agagaatgga tcccaatcat ttaaaactag agaaccaaga
     3781 tcgctatatc gatcaattgg tatttacgtt tagacgatta ttgaagagtg attgtcatgg
     3841 ctaaaaatag ttgggagcta aaaataaatg gacatgatga acttcttgtg cggatggaac
     3901 gctattcaag cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa ggttcggcta
     3961 ttgcagtgga taggattaca gaaaaaattc ctgtttctga agcagattta agaagagggc
     4021 accaacacgc aaaaaatagt cgtccactta agactcaata cattaatttg ggtttcatca
     4081 ttagacctac aagaaaattt gagtatttaa aatatcctga tttggggata ggtacttcta
     4141 aaagaaatca gccggacgaa tttatgagaa gaggattagg tcttgcactt gatccaatta
     4201 cagaacttct gattcgtcaa ttcgataaat taaataaata gggggaacaa caatggctaa
     4261 aacaaaaact gtagtaacaa cgttcgataa cgtaagtatc aaacgaattg cttttaattt
     4321 taagaacgca ggaaatgcaa tcgcaacgaa ttgtaatgga caattagatg gcgaaacaga
     4381 aatgcaaacg gtggttaaaa aatgtggagc gacagaagta aaatcaaaat ctaaaccaat
     4441 caatatgacg gtaacaatta ctgcacatgt accgatggaa gtttatcgac gtttcaatgg
     4501 gttgaaacaa gatggacgta ttaaaccagg catttactct tacggtcctg attccgtagg
     4561 cgaagatttc tcacttgctg cagagatcgt ggatgacttc gaagaaaata gaaagttagt
     4621 tggtatgtta gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga
     4681 tgaagtagct gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta
     4741 tcatgaagca attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct
     4801 atctgctgat gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta
     4861 aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa
     4921 caattttgaa tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt
     4981 ttaacacaga cgagtttgca aaaatgactg tgggaaaagg atccatggat atttctgtta
     5041 ttcaaggtgc aaaggctgtg tatattgctt accgccaagc gaacatgact gattatattt
     5101 cattcgatga atttatcgat aaatgggatt ttgatatggc tactgccagc tatatttatc
     5161 aattgatgat gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata
     5221 aggaaaaaaa gcttcaaaag taaaaattcc aaagctctta gttgaaacgt gggtcgatgt
     5281 ctattcgatg ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg
     5341 tttagatgac attttgcaaa tggcttacaa caagagtgct tatgaaggat ggaaaaacta
     5401 tgcaataaac caatcccaga aaaactaaag aaaggaggta aaaaatggct aaaaagagaa
     5461 cagaagcaga agtaactttc atagctaacg atgacggatt gaaatctacg ttaaaagaaa
     5521 tcagtgctga attaactaaa aatagagcag aattaaaact agaacaagct caattacaac
     5581 agactggttc tgaatcagac aagttaggaa gtaaattatc ttctttagaa aagcagtatg
     5641 aattacaaag tcaaaaagtt gaagtaacta gccaacgttt agccaatgcg aaaaaatatt
     5701 atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga actgattaat caacaaacag
     5761 cgcaacaacg tttgtcaaac gaaattgata aaacgagtaa tgcactagct caagcaaaag
     5821 gcgaaataca gacgtacgag tctacaatgc aacagttgga tagcgaacaa aaaaatgttc
     5881 aagctagtgc ttctctgatt gaatccgaat acaaaaaatg gcaagcaact gctggtcaat
     5941 cagcttctga agccgagaaa ttagcgaaag cccaagaata tgtttctcaa caatctgaaa
     6001 atgcggagaa aacgatagat atcctaagac gacagttaga agctacacag tctgaatttg
     6061 gcgctacatc cacagaagca atgcagatgg aagcgaagct taatgatgct gaacgtgaat
     6121 ttgaagagtt aggacaagct gctaaaaatg tagatacaac taacttggac gatatcggaa
     6181 gcaaaataga tatgaataat ttaatggaag cttctgacgt tttaagcgac attggcgata
     6241 agcttacaga attagggaaa caagcagtgg actctgctaa cagtgtaggt agttcccaga
     6301 gtaagataca agctaatttt ggtttgtcta aacaagaggc tgaagaatta acgaatgtag
     6361 ccagagacat ttattataaa ggttttggag aatcgttaga tcagtccaca gatgcattga
     6421 ttttggtaaa gcgtaattta ggagatttaa ataatcaaga tttacaaaat atcacggaac
     6481 aagctatggt cctagaaaac accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa
     6541 atggcttaat ggtcaatttc ggcttgag
//
LOCUS       DACDVT010000103         6393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDVT010000103 DACDVT010000000
VERSION     DACDVT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.105"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            65..481
                     /locus_tag="IWL52_002412"
     CDS             65..481
                     /locus_tag="IWL52_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9778928.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            501..2045
                     /locus_tag="IWL52_002413"
     CDS             501..2045
                     /locus_tag="IWL52_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9778929.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            2188..2412
                     /locus_tag="IWL52_002414"
     CDS             2188..2412
                     /locus_tag="IWL52_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778930.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            2427..3296
                     /locus_tag="IWL52_002415"
     CDS             2427..3296
                     /locus_tag="IWL52_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9778931.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3277..4011
                     /locus_tag="IWL52_002416"
     CDS             3277..4011
                     /locus_tag="IWL52_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9778932.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4044..4952
                     /locus_tag="IWL52_002417"
     CDS             4044..4952
                     /locus_tag="IWL52_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9778933.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4949..5491
                     /gene="sat4"
                     /locus_tag="IWL52_002418"
     CDS             4949..5491
                     /gene="sat4"
                     /locus_tag="IWL52_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP9778934.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5584..6378
                     /locus_tag="IWL52_002419"
                     /note="aph(3')-IIIa"
     CDS             5584..6378
                     /locus_tag="IWL52_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9778935.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2215 a         1002 c         1466 g         1710 t
ORIGIN      
        1 cagggatgag ctgacattca atttgaaatg cggtctttcc ctgaaagaaa aggtggtgag
       61 ataaatggca tatattccat atggatacaa aattcaagat ggagtggtta ctgtcgatga
      121 aaaggcagca ggtcaagtaa aggtattctt tgagaaatac atatcaggac tatcccttac
      181 agtggctggc gaacaggcag gtattgaaaa gacacattct gtgatggggc gcattttgaa
      241 aaacgtcaac taccttggaa atgatacgta tccagcaatt attgataaag agatatttga
      301 taaagctgaa gaagttagag ataaacgtgc aaaggattta ggacgagtgg tagagcttgc
      361 cgctttcacc tctccccctc ccaaagaacg attcaaaatg aaaaaggcag ataataagat
      421 gccagtggat ccttttgaac gagcagaata cttatacagt ctgatagaaa gcgaggaata
      481 aagtgacaga gaaaaatata atggttattc ctgctcgtaa aagagtagga agtacagccg
      541 caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg ccgtgtttct acagaaacag
      601 aagaacaaaa ttctagctat gaggttcagg tctcacacta tacagagttt ataaagaaaa
      661 ataatgaatg ggagtttgct ggcatctttg cagatgatgg tatctctggt acgaacacta
      721 aaaagcgtga cgaatttaat cgcatgattg cagagtgtat ggacggtaac atcgacatgg
      781 ttattacaaa atccatcagc cgatttgcgc gtaacaccct agactgcctt caatacatta
      841 gacagctcaa ggataagaac atatcagttt attttgagaa agagaacatc aacaccacgg
      901 atgccaaagg tgaggttttg ctgactatta tggcatcttt ggcacagcag gaaagccaga
      961 gcctttcaca aaacgttaag cttgggctac agtaccgata ccaacaagga aaggtgcagg
     1021 tcaaccataa gcgatttatg ggctacacga aagatgaaga tggaaattta actattgtcc
     1081 ccgaagaagc tgagattatc aaacgcatct accgagaata ccttgagggt cagagtctag
     1141 tgggcattgg tcgagccctt gaaaaggatg gtattttaac agcagcggga aaaccaagat
     1201 ggcgaccaga atcagttaag aagatacttc aaaacgaaaa atacatcgga gatgcccttc
     1261 tgcaaaagac tgtcacagta gattttctga ccaagaaacg agtgaaaaat gaagggcatg
     1321 ttccccaata ttatgttgaa aatagccatg aagcgattat tcctaaagag ttatttttgc
     1381 aggcgcaaga agaaattcat cggagaagca atatctacac aggagaaggc aagaacaaaa
     1441 gaatttacag tagcaagtac gctttaagtg ccatcacctt ctgtggagat tgtggcgata
     1501 tttatagacg ggtctattgg aatatccacg gaagaaaaga atttgtctgg cgatgcgtga
     1561 ctagaatcga gcaaggtcct gaagtttgta agaaccgaac cgtaaaagaa gatgaacttt
     1621 atggtgcagt aatgactgcg attaataagc tacttgcagg tgggaacaat atgataaaga
     1681 cactggaaga gaatattcat gctgtgattg gcgaaaccac agaataccaa atttcagaga
     1741 ttaacaactc actagaggaa aaacaaaaag aactaatcaa gctggcgaac aagggtcaag
     1801 actatgacca cttagcagat gagattgatg agctgagaga caagcgacag atccttttag
     1861 tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc
     1921 gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag ataatccaga
     1981 gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc gaaatggaaa
     2041 tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt tttcttttgc
     2101 ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg gtttataata
     2161 ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
     2221 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac tccacagaat
     2281 ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc cattgctgat
     2341 gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga aataaaaata
     2401 tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata gtaacagaga
     2461 aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt gcaagaggca
     2521 cccatacaga aaattctgat atagatatcg gaatctatta caattcagac tcatttgacc
     2581 tgacagcgat taatcaaatt gctacagaat tggatgataa aaatagaaac aaccttgttg
     2641 tgcctcccgg agcgtggggt gattggatta atggcggcgg atggttattt ataaatgggt
     2701 atcatgttga cttgatttta cgtgatataa aacgggtaga acaaataatc aaagatacgg
     2761 agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat ataagtgcaa
     2821 tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa agcttatgcg
     2881 aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg atgaactttt
     2941 ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg gtagaggata
     3001 aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa gtactatttg
     3061 catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt gaaacttttg
     3121 aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta ctcggtcttt
     3181 ctctttttga atgctacgat atgaccgaga aactttataa agaagtgaat gaaattgtat
     3241 cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat
     3301 aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg
     3361 ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat
     3421 ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta
     3481 gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca
     3541 cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat
     3601 gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag
     3661 atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt
     3721 actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg
     3781 gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca
     3841 aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata
     3901 aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat
     3961 gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga
     4021 aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt
     4081 ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata
     4141 ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag
     4201 aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa
     4261 agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat
     4321 ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc
     4381 taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca
     4441 tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt
     4501 gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa
     4561 ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat
     4621 catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta
     4681 ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt
     4741 cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg
     4801 gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat
     4861 ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg
     4921 ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa
     4981 agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
     5041 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
     5101 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
     5161 taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa
     5221 aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca
     5281 aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca
     5341 tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg
     5401 tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga
     5461 aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt
     5521 cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
     5581 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
     5641 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
     5701 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
     5761 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
     5821 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
     5881 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
     5941 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
     6001 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
     6061 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
     6121 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
     6181 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
     6241 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
     6301 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
     6361 ctggatgaat tgttttagta cctagattta gat
//
LOCUS       DACDVT010000104         6358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDVT010000104 DACDVT010000000
VERSION     DACDVT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.106"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..94
                     /locus_tag="IWL52_002420"
     CDS             <1..94
                     /locus_tag="IWL52_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAP9778936.1"
                     /translation="IKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            96..611
                     /locus_tag="IWL52_002421"
     CDS             96..611
                     /locus_tag="IWL52_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP9778937.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            627..1289
                     /gene="rpe"
                     /locus_tag="IWL52_002422"
     CDS             627..1289
                     /gene="rpe"
                     /locus_tag="IWL52_002422"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9778938.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            1296..1868
                     /locus_tag="IWL52_002423"
     CDS             1296..1868
                     /locus_tag="IWL52_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9778939.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2055..3575)
                     /locus_tag="IWL52_002424"
     CDS             complement(2055..3575)
                     /locus_tag="IWL52_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9778940.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(3818..4003)
                     /locus_tag="IWL52_002425"
     CDS             complement(3818..4003)
                     /locus_tag="IWL52_002425"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP9778941.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(3987..4169)
                     /locus_tag="IWL52_002426"
     CDS             complement(3987..4169)
                     /locus_tag="IWL52_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778942.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            4410..4628
                     /locus_tag="IWL52_002427"
                     /pseudo
     CDS             4410..4628
                     /locus_tag="IWL52_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            5123..5722
                     /locus_tag="IWL52_002428"
     CDS             5123..5722
                     /locus_tag="IWL52_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9778943.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            5786..>6358
                     /locus_tag="IWL52_002429"
     CDS             5786..>6358
                     /locus_tag="IWL52_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778944.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         2093 a         1095 c         1163 g         2007 t
ORIGIN      
        1 aattaaagct aaagtaacga atggcttggg cttattaaca ggtattggta cagatgccat
       61 ttatgcagat gtaaaagaaa gattaggtgt ttaatatgcg ttttcatcat gacactttga
      121 ctattgaaac agtttcaggg cgtccttcgt atcatgatat tcgaaaagaa cttttagata
      181 cagttgatac agcaggtatt gagaatggaa tgttagtaat ttcgagcccg catacaactt
      241 gctcactctt ttttgaagaa acgatgcatg acacaaactt ttttggtgat gattatttac
      301 atgtagacat caacaatgta atggaaacga ttattccaaa aatgacgagt gaaaatcaat
      361 acaacagtcc ggggccaaaa cacattgatt ttggaacaag attatctgat cccaattacc
      421 ctgcagaaaa atgggtcatg ctaaataccg atgcacattt acgttcatct atttatggaa
      481 ccaactcaat gactttaatt gttcaagaag gtaaactttt attaggagaa atgggccgtg
      541 tctattttgt cgattgggat caactaagag agcgaactag aacaatcaat ctcatgatta
      601 tgggagaata aacgggggaa tagagaatga caattgctcc atcattgtta aatgcaccta
      661 tcgcagaagt taaagagtta tttgaaatct taaaagaaaa gaatgttgat tatttgcata
      721 ttgatgtaat ggatggctgt tttgtaccag atatggcttt tggaccaagt gttattaaag
      781 atattcgtcc actaacagac gttgtgtttg atgttcattt gatgattgat acaccagaaa
      841 aaattgtcac aagatttgta gaagcaggag cagatattgt taccttccac attgaagcta
      901 ccaaacacgc aatgaaggtc attcagttag tcaaggaagc aggagcaaaa gtagggatta
      961 cttttaatcc tgcaacacca gtcagtttta ttgaaccagt attatcttta gtcgatcaag
     1021 tattagtaat gacaatcaat ccaggaacag agaataagca ttttattcaa gagacagtag
     1081 ttaaaatcga acagttagat gtaatacgca aacaaaatga ttatacgtat gatattgaag
     1141 tagatggcaa gattgataat caaacgatta aagtttgttc taaagcaggg gcggatattt
     1201 ttgtatcagg cggtttcatc ttcaatggga aaaatacccc gcaagaacaa attgataaac
     1261 taaatcaatc attagaggaa gtgaagtaaa agatcatgac caataaagac caaaataaaa
     1321 actatttaga agcgattatc ttagaattac aaaataactc aaaacgtatt aattctgtag
     1381 agttagaaca atttagtgag aaaattgttc aagctaaaca catctttcta gctggtgcag
     1441 gacgctctgg aattgccatg caagcattta cgaatcgctt aatgcattta ggtttttcag
     1501 tcagccttgt cggtgaaatt agttcaccac attcgcaacc tggagattta ttaattatct
     1561 gttctggctc aggtgaaaca ggtagtttaa aaaatttagc ggccaaagca aatcaaagtg
     1621 gtgtaagcat tgctttagtt accatgaaaa aagactcgtc gattggtcaa ttagcagatt
     1681 ctgttcttgt attaccaggt attaccaaag atgaaaatca acgagaagaa gatacttttt
     1741 cacaacctat gggttcaagt tttgaacaat tagcatttct cacctttgat agtttagtgt
     1801 tatctttaat ggataaaaca ggtgaaagct cagaaacgat gtttgctcga catgcagatt
     1861 ttgagtagta gattttaaag ataaaaaacc attctctaaa tgctaattat tcagcaaata
     1921 tgagaatggt ttttttattt gattagacat aacttaataa ttcagtagtt ttgagcgtaa
     1981 gcgtgtcata gaatggtacc taaacacaaa tagcccgatt tgatacactc aaatcaggtt
     2041 atttgtggct tatattattt acagatttct tgtatctgct gattccatgg caaataagcg
     2101 cgtagcgctt caggtgtcat cgaagacaga ttgggaatct ctgtaaaaag atgatttaaa
     2161 tatttgcgga tgtttaaccc attcgcaaca gctgtctgga taatactcaa tataattcca
     2221 tttgatctag cgccttttaa gcttttagaa aatagccagt tttttcgacc gatcactaac
     2281 tctttgatgg cacgctctgc tcggttattt gaaagttcca agcgtccatc aagcaacaca
     2341 atctgaagtc cgtctttatg attgactgcg tattgaaccg cctttccaag ctgcgatttt
     2401 ggcaaataag caaaactctc acaccaatga aaaaattcag tcatcacagg ctgcaacgtt
     2461 tccttcctcg ctacaaacct ttcttctgcg ctaagcttat taaaggtccg ttcgattcga
     2521 aacatcttgt cacagaagtc taatcctatt ttggcatgac tttttgggga cggttgttta
     2581 cccaatgctt caaaaaactt tctgcgaaca tgcgcccaac aataaacatt gtttaccggc
     2641 agctttccat aggccgcgta accatcagta tgcaagaaac cgctaaatga ttctaaaaaa
     2701 ctttttggct catcaacgga acgcgaattt gcgtgctcgt agagcacaac ttgtttttct
     2761 tcgcttttac ctgaagcata taaccagtaa aaggtctttt ctttatgagt attcaatacc
     2821 cgatagcttg tctcatccgc gtgtaagacc tcctgattgc ttaattcttg atgaaggcaa
     2881 ttgtagattg gctctaaaac catttcactt accttgatat gccaattagt gattttttta
     2941 cgatcaagag ggaaaccaag tgcatgccat tctttttctt gtcgatatgc cggcaccttt
     3001 ttaacaaatt tttcccaaat actctgtgtc acaattgaag aggaacctag actattattg
     3061 atcaacggtt taggaacagg cgcttttttg atttttgttt ctcctaatgc ttcacattct
     3121 ctgcaatgat aggcatgttg gtagatgact tgtttttcta tatgggcagg aacatacaat
     3181 ggctcttctc taagccttgt cgcaccgata tcatgtaaag tatgaccaca gtcttcacac
     3241 aaacaatcgg caccttttaa ttcataatga ataggaactt caggcagacc ttctaatgaa
     3301 agtgctttga gtcccttgcg ttttctgcgt ttaaaagaag ataccgtaat gggttcctgg
     3361 ctttcttgtc cagtgtgctc tggttcatta aaagttgagt cgtctaataa atcgatttgc
     3421 ccatctacgg gtggtgtttc tttttttcgt ccaaacaagc gtcgattcat ttcagcaatt
     3481 tgttctctaa gaagagtatt ttcctgttgc tggatttcaa gcaactgtgt taattgcttg
     3541 atttgtacaa gaagttcaga ttccattttt gtcattttta caccaccatt ctagcgattt
     3601 tcttacatct attataccat tttatgacaa cgaaaaacag acaaaatcct tgatttaaca
     3661 aggttttcgt ctaatatatc gcaccttttt tcgtttcttt tagcgtacat ttttcttcga
     3721 ttgaaaggcc agtcagtaag acttcttttc tggaacgcgg ccattgaagg tacccacttt
     3781 caaatctttt gtacattaat acaaatccat tttgatccta gtaaagagcc ttgaagcgat
     3841 ctttcttcgt accgcaaaat aagaaaagcg cttgatcgta aacatcaagg tcatattctt
     3901 ctataacgaa agctgctagt ccatcaattc cttttcgaag atctgttttt ccacaaacga
     3961 taaaaatatg tttcaatgca gttgcatcaa tgagcattgg cattcaactc ttttaacaag
     4021 gtatacaaaa gatatttatc cattccatta taaataaata tttccttatc ttccgatttg
     4081 attctgcaag ctagttttgg cttggtgtca atttgcgatt tttgagccgc acttgaaaat
     4141 tgaactggta caatatcttt tttattcatt cgtttttctc cttctatatt tgatagttta
     4201 attatagaag aaagataatc ggcgtacgag atactatcct atgacacgct tacatttaat
     4261 ttaatatttt gcctcaaatt agccacactt tttatagaac taagaggaaa aatgaagttt
     4321 tcttcctatt aatatagttg tttgaaatct taaatatagc gttttacgat gtaatgggaa
     4381 gtatggtgaa taaatcaact atgttcgtac gaagcattta ctcaactaat ttaatcgaat
     4441 ctttcaataa acaaatcaag aaatacagcc accgcaagga acagttccaa aatgaagagt
     4501 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
     4561 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
     4621 agaattagaa acaatctaca gtagggaaga accattgaca caaaattatt gacgctccca
     4681 actaaaagtg tcaaaataat gatgaaatca tttagttctt ataaattaga aaagagaaaa
     4741 taaatattcc atatctactt tattggatcc gtaacatgac taaattctga atacttttta
     4801 aagcttatca tttggtaaaa ctgaatataa gttaattata tagactgaaa attttgaatt
     4861 ttcagaatag aataacttaa aagtactgac aaaatttttt aaaggggcaa tattaatgtt
     4921 ctgagataat caaatcctac cgaaaatatc aaaaatccaa tgtgacacaa tcggattgtg
     4981 tctcattcga gggaatcaca caatcccacg tggctctagg cagaattgaa tttttgcttg
     5041 gggctttttc cttatattga ttttgaatga gacttattat atgttataat gcaattaagt
     5101 atcattggag agggcgaaga aaatgaagac ggtagaacct attcgagaca aaaaaaagat
     5161 cgatgcgatg aaggcaatct tggcttcggg aaaatatggc caacgaaatt tggttctttt
     5221 ttcgattggg atcaacaccg cctatcgcat ttctgatttg cgtcaattga agctggccga
     5281 cgtgctggaa atttcacgcg gtcgcgtggt ggtcaaagat cgattagcga tgaaggaaca
     5341 aaaaacagct aaacataatt cggtctatat ttccaacaaa ttgcgaaaaa cgattttgga
     5401 gtacgttcaa caggaatttc ctgatcaact ccttcagcaa gattttgacg gttatttgtt
     5461 ccctagtcga aaagggacga atgaaccgct caaccgccaa agtctgtggc gaatcattca
     5521 tgatgctggc acggcaatcg gattgaaaga cattggccca cattccatgc gaaaaacgtt
     5581 cggttacttt ctctataaac aggggaccaa aacagaaatc attcagtcct tgttgaacca
     5641 ttcttctcag cgcgaaacat tgcggtatat tggtattaca caagaagaca aagatacagc
     5701 tgtaaaaagc ttagatttat aatataaaag ttaagtttaa acatggatag ttttaaatta
     5761 gtagattgag tgttgggagg aaagaatgag tacattatat attataggaa acggttttga
     5821 tttaagtcat ggtttaaaaa ctagatacac tgatttttca aaatgggttg aaaaaagtca
     5881 tagtgaaata ttcgataaaa tagacacaat gataaatgaa aataatgagg tgagaagtac
     5941 agctgatgat aagataggtt ggtttaattt tgaaagtagt ttgcatgagt taattcaaag
     6001 tccagtcttt gaagattatg tagaagagtc taaggattat ctcataggtt atggacatga
     6061 agattggtca gattctgccc atcatgattt tcaaaatatg gtttatgatt atgttgtttc
     6121 tctagaagaa ttacaacaac ttttcaagga atggatacgc aaagaaatac agccgacaat
     6181 agaacataaa attccaaaat accaattttc gtccgatgta ctattcttta cattcaatta
     6241 tacagatgtt ttggaaattc tatacaagca accgaaagaa aacatatatc atattcatgg
     6301 cagttatgac gatataataa tcggccactc cgagaatttt gacaacttta taggagat
//
LOCUS       DACDVT010000105         6358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDVT010000105 DACDVT010000000
VERSION     DACDVT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.107"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            93..1688
                     /locus_tag="IWL52_002430"
     CDS             93..1688
                     /locus_tag="IWL52_002430"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9778945.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            1678..2895
                     /locus_tag="IWL52_002431"
     CDS             1678..2895
                     /locus_tag="IWL52_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9778946.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2908..6057
                     /locus_tag="IWL52_002432"
     CDS             2908..6057
                     /locus_tag="IWL52_002432"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9778947.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2220 a         1048 c         1315 g         1775 t
ORIGIN      
        1 tctttctttt tttttttgct ttttataaaa gggggtatgc taaaataaga gggtatatag
       61 caactttaaa taagagatag gagtgtatct ttttgggtgc agaattaaat caaaaattat
      121 ttagtgcggc agataatctc cgtagtaaaa tggatgcgtc agaatataaa aattatttat
      181 tgggactgat tttctataaa tatttatccg atcgattgtt ggaacaagtg gttcttttag
      241 cagatgaatc cttagaggaa tatgacacag taagcaaaca aacaatgttg tatcgtgaat
      301 tactttcaga tgaagaatca aaagaagatc tgattgccac aatcgttgat attttgggat
      361 atgcaattgc accagagtat cttttcaacg tcttagcgga tcaagcaaaa caagcaacgt
      421 tccaattgaa cgatctaaac aaagcctttg tgcaattggc atctacctac aatcaattta
      481 atgggttatt tgatgatgtg gatctccaat caaaaaaact aggaacagat gagcaacaac
      541 gaaatgtgac gattaccgaa gtcatcaaga aattgaatga tgtagatgtt ttagaacacg
      601 atggggacgt gatcggggat gcgtatgagt ttctgatcag tcaatttgct tcagaagcag
      661 ggaagaaagc cggagaattt tacacacctc atatggtttc tgatatgatg gcgcaaatcg
      721 taacgctcga tcaaaaggaa cgacgattct ttagtgtttt tgatccaacg atgggttcag
      781 gttcattgat gttaaacgta cgtaactact taacacaccc agataacgtt aaatatcatg
      841 ggcaagaact aaatacaacg acatacaatc tagcaaaaat gaacttgatt ttacatggtg
      901 tcgacgccga agaaatgaat ctccgcaatg gggatacgtt gaataaggac tggccaacag
      961 atgaacctta cacatttgat gcagtagtaa tgaacccacc ttattcggct aattggtcag
     1021 cagatacgac tttcttagat gattctcggt ttaatcgtta tgggaaatta gcgccaaaat
     1081 ccaaagcaga ttttgcgttt cttcttcatg ggttctatca tttgaaggaa acaggtacca
     1141 tggccatcgt cttaccacac ggagttttat tccgtggcgc tgcagaaggg gtcatccgtc
     1201 aaaaattact agaagatggc agtatttatg ctgtgatcgg tatgcctgcg aacttgttct
     1261 ttgggacatc gattccaacg acggtgattg tgttgaagaa gaatcgtcaa actcgagatg
     1321 tattattcat tgatgctagt cgtgaatttg ttaaagggaa aaatcaaaat aaactatcgg
     1381 aagaaaacat tcaaaaaatc ctcgaaacct acgctgaaag aaaagatgtc gagaaatacg
     1441 ctcatttggc aacctttgat gaaatcaaag aaaatgacta taacttaaat atcccacgct
     1501 atgtagatac tttcgaagaa gaagaaccaa ttgatatggt tcatgtcgga aatgacatca
     1561 agaagatacg acaagaacag caagtgctag aaaaaaaact tttagaagct atctcttccc
     1621 tgcaaacaac acctgaaaat gaagcgtggt tacaaggtgc gctggaggtg ttcaaacatg
     1681 agcaatgata cacaaccaga aatccgtttc ccaggattta cagatgattg ggaacagagg
     1741 aagttggggg acttcgttgt agattatgtt gaaaagacaa gtgtacaaaa tcaatttcct
     1801 atgctaactt catctcaaca aaaagggata gttttgcaag aagactattt tgcaaatcgg
     1861 caagtaacta cagaaaataa tattggttac tttgtactac ctagaggata tttcacgttc
     1921 cgtagtcgaa gtgataatga tgtgtttgtg tttaaccgta atgacattat tgatagaggg
     1981 attataagct atttttatcc tgtatttact ttaaaatctg ctgattccga cttcttctta
     2041 agacgtatta acaatggaat ccaacgacaa ttatctattc aagcagaggg aacaggtcaa
     2101 catgttctgt ctctaaaaaa gtttaaaaat atagtagcaa tgtttcctag tgaggaagaa
     2161 caacaaaaaa tcggcacctt cttcaaacaa cttgacgaca cgatcgctct tcatcagcgt
     2221 aagttagatt tgctgaagga aacgaaaaaa ggattcttac aaaaaatgtt ccctgaaaac
     2281 ggagcaaaag tgccagaaat ccggtttcct ggttttacgg aagattggga agagcgtaag
     2341 ttgggagaat taggtaaaac tcaatcggga attggatttc ctgatgccga gcaaggtggt
     2401 tctgaaggta ctccattttt taaagtttcc gacatgaata acattggtaa tgagtatgaa
     2461 atgagaaatg caaatcatta tgtatctaat gagcaaattg agcgtaaaaa atggaagcca
     2521 ataaaagatg tgccagcgat tatatttgct aaagttggcg cagcaataat gcttaataga
     2581 aaacgtttgg tcacaagccc tttcttaata gataacaata ccatggctta tttatttaat
     2641 aatacttggg atatctattt tggtaaaata ttgtttgaaa ctataaattt gccaagatac
     2701 tcccaagtag gggcattgcc ttcttataac agttctgata ttgagaatat cagtgttaag
     2761 gttcctgtaa aggatgaaca acaaaaaatc ggtactttct tcaaacagct agatgatacg
     2821 atcgctcttc atcaacgtaa gttagattta ctaaaagaaa cgaaaaaaag cttcttacaa
     2881 aagatgtttg tctaggaggg aagaagaatg aaaattagtc accgtgagga agcagaagtc
     2941 gaagaacagt tgattcgtgt acttggagaa gggcataatc agtggacgta tcgtccggat
     3001 ctaaaatcag aagaagacct ttgggtcaat ttacgccaaa aaattatctc aaataatcaa
     3061 gcagaactga atgattttcc tttgaccgat aaagaatttg aaacgatcaa aacggaatta
     3121 ctgctgcgaa cgaaaacccc atttgatgct gcgaaatggt taaaaggtga aaatgggatg
     3181 gctcggatca cgattgaacg ggaagattcg caattgggtt ctgtctcact tatcctttat
     3241 tccaatcaag acattggtgg aggaatctct acttatgaag tggtccatca aattgcaaaa
     3301 cgtggaagta acatagaagc acgggatcga cgttttgacg tcaccttatt aatcaatgga
     3361 ttaccgattg tccaaatcga gctgaaacaa gtaactgcaa aagatggtgt ttatcaggca
     3421 tttaaccaaa tcaaaaaata tgcagaagaa ggtatgtttc ggaacaatat cttttcgacc
     3481 ttacagttgt ttgtggtttc caatgaacaa accactcggt attttgcaaa tgccttaccg
     3541 aaagacttgc accctaagtt tttatttagt tggcgaacga aagacaatga aaaagtagaa
     3601 aacttatatg agttttgtaa acaagtgtta aatatcccgg atgctcatcg tttgattgcg
     3661 gattatacaa tcgtcagtga agatcaagac aataaaaccc tcatggtgtt acatccttat
     3721 caagtacatg cgattcaagc cttgtttatt gccgcaaata aacatcaatc agggtatgtg
     3781 tggcatgcga ctggttcagg aaagacgttg actagttttg tatctactaa gcttttagca
     3841 cgaaaatcgg ggatcgatcg aacgatcatg ttagtggacc gcaaagattt agataatcaa
     3901 accaccacag agtttacaaa atttgcttcg gaatttaata ctgggatttc ttccgggaac
     3961 gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag tgaaaccctg
     4021 ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact agatgcggcg
     4081 ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca aaaattaatg
     4141 ggccaacata tcgtgtttgt agtagatgag tgtcatcgag cgttaagtgc tgaaaatatg
     4201 gaagaaatca aaaaaatgtt tccgaaatcc acttggtttg gctttacagg tacaccgatt
     4261 tttcctgaga atcggaaaca agccaaaggg caattggcac gtacaaccca tgatcagtat
     4321 ggagaggtct tgcataccta taccattaag aatgcgctag aagatggctc tgtcttagga
     4381 tttcaagtag agcatgaaaa caccgtggag ccgacttcac tagagaataa aatctatcgg
     4441 aaattaaaag aagtagaaac atatgcagag tattcatcag aacagatcaa tcgtatgatt
     4501 gatcaaatgg aacctgtcaa aaaagaaagt tatttggacc cagctgtttt tgaagcagat
     4561 gaacatatcc aaaaagtcat tcataaaatg ttccgtccag ataatgccta tacaaaattt
     4621 gattttcgaa atggcaggcc aacgaaatct gcaatcttaa ccacgagttc cattgacatg
     4681 gccaaaaagt attatcgggc catcaaagag atgacgaaag agcctgattg gttaacaaaa
     4741 gagtttccca atcagctcat tcgtgaagga cgaacgatgg aggatcctga ttttccacga
     4801 atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca acaagaagaa
     4861 atgcaaaaaa ttattgagga atataatcag tattatgata cttcctggtc attagcagac
     4921 attgagcgtt ataacggaga catcaataat cgtttggcac gaaaaagagc ggaattcaaa
     4981 caatttggga aacaaatcga tttagcgatt gtagtggatc gtctattaac aggttttgac
     5041 gcccctacga ttcaaaccct atttgtggat cgtaatctag agtatgcagg attgatccaa
     5101 gctttttcac gtacgaatcg tacgtatcct gataagacaa aaggattgat tgtgacgttt
     5161 cgtaagccag cgacaatgga gaagaatgtc gaggatgcga caaaactgta ttcaaaagca
     5221 aaagaagaat caggtttgat ttatccaagt tatcaggaat ccaaaaaaag attcaaacaa
     5281 gcatatggta agttaaaaga ggtttcatcc atggaatcca ttgatgaaca tacgccactt
     5341 gaaatccgta tcgagtacgt caaagcattt caagaattga atcgtgctta tgaatcgtta
     5401 gtgacttatg acgattacaa tgatgacatg gtgaaatctc caactttgaa caatcaaatt
     5461 caacttttgg aagaacaaat tggcgtgtac gagaccgtga agggttcatt aatagaggaa
     5521 gaaccagaaa aagaaggaga agatttttca acgatcgaat tttatagtga aaataccact
     5581 aagttgtatg atattgattc aacttatatt gatcaattac taggaactta tgctgcgaat
     5641 agttcagatg cacgagaaga aattgaaaaa gctttggcta aattgaataa aacagaagga
     5701 gtcaaagaag tttaccgaca aattttaaat gcatttgatg tggggacttt agatagtaat
     5761 gaagatatct ttgtcattaa acgtcgctat tttacacaag cgagtgatga tttgattcat
     5821 gtcttttcca atgagtggtt tgtttctgag aaagaattac atgcttctaa tctgcaatat
     5881 ctaccaggtg aagatccaat tcctaatatg aaagcaatta tcaacagtaa ggattacgaa
     5941 ggatataaag ccaaacatcc agaagcgaag ccatttaaat acccacaaga aatgaagcga
     6001 gcatggcgta aaatgttaga tgatgagttg attccacttg agaatgaatt gaggtaaacg
     6061 agaagagtta agaaaattgt acgattcaaa gatggtagtc aagtggaagt gtagaaaata
     6121 gggatagatg tcttttattt gttcacattt tataacgaag tatttaagac agctattgaa
     6181 taatgtttcc tccacctttt tcattcattt accccacccc gttgttatat gaccgtgttt
     6241 tgagaggaac caataaaaag cataggctaa ctaggggttc ctttttcttt tgtttttttg
     6301 gaatccgctt aagtgaaatg tatctgaaat gaaacggaga taaagtgatt ttttgcta
//
LOCUS       DACDVT010000106         6348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDVT010000106 DACDVT010000000
VERSION     DACDVT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.108"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1464
                     /locus_tag="IWL52_002433"
     CDS             <1..1464
                     /locus_tag="IWL52_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9778948.1"
                     /translation="RRDSVVVALTPIPKQTQLTIDQQTITTLEDIPQGHKIALGDLKA
                     GDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVHPSHYLFENRTFQ
                     GYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFDHITILKHPYGCS
                     QLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGEYDEERVKFLVAQ
                     DVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLSGITANPLLGAFS
                     DYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFKQYFLSYGEPVYE
                     NPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLSLLQAPGNDLVAA
                     SALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDFNAGVLLEQPMEV
                     ILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            1503..2897
                     /gene="uxaC"
                     /locus_tag="IWL52_002434"
     CDS             1503..2897
                     /gene="uxaC"
                     /locus_tag="IWL52_002434"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9778949.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            3056..3739
                     /locus_tag="IWL52_002435"
     CDS             3056..3739
                     /locus_tag="IWL52_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9778950.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            3741..4577
                     /locus_tag="IWL52_002436"
     CDS             3741..4577
                     /locus_tag="IWL52_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9778951.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            4588..6342
                     /locus_tag="IWL52_002437"
     CDS             4588..6342
                     /locus_tag="IWL52_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9778952.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         2025 a         1227 c         1273 g         1823 t
ORIGIN      
        1 cgacgagaca gtgtggtcgt cgcattaacc cctattccaa aacaaacaca actgacgatt
       61 gatcagcaaa cgatcaccac tcttgaagat atcccgcaag gtcataagat tgcattgggt
      121 gatttgaaag ctggagacaa cgtgatcaaa tatggctatc caatcggtca tgtgacgaca
      181 gacgtaactg ccggtcaatg gctgcacact cataatgtaa aaaccaatct ttctggtgaa
      241 ttggattatc gatatgaaaa agatgtccat ccaagtcatt acctttttga gaaccgaaca
      301 tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc gtaatgattt gtttttagta
      361 cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt caaggaaaaa
      421 catccagatc tgggacagtt tgaccatatt acgattttaa agcatcctta tggctgttcg
      481 cagcttggaa aagatcatca aaatactcga gaaattttag cggacgcagt aaatcatccc
      541 aatgccggag gagttttagt atttggatta ggttgtgaga acaatacagt tcctgaattc
      601 aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat ttctagtggc tcaagatgtt
      661 tacgatgaga tcgaacaagg tgtggctcta ttagaagagc ttttaacagc ggcggaaaat
      721 gaccagcgca tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg cggcggttct
      781 gatggtctat cgggaataac tgctaatcct ttacttggtg cattttctga ctacttgatt
      841 gctcaaggag gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc agaacaagta
      901 ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt gatcaatgac
      961 ttcaaacagt atttcttgtc ttacggggaa ccagtttacg aaaacccatc tcctggtaat
     1021 aaagcaggtg ggatcactac attagaagat aagtctttag gctgcacgca aaaatcagga
     1081 agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaacc agggcttagt
     1141 ttattgcaag ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc atccgattgc
     1201 cagttggtct tgtttaccac aggaagaggg actccgtttg gctcatatgt cccaacggtc
     1261 aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc attggatgga ttttaatgca
     1321 ggcgtcttat tagaacaacc aatggaagta atcttagaat cgtttgttca aaaaatcatc
     1381 gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga aattgccatt
     1441 tttaaaaacg gagtaacact ttgattttca taaaacagta taaaaagaaa gaaggaagat
     1501 ttatgttttt atctgatgat tttttattaa aagacgattg ggcgaaaaag ctttaccatt
     1561 cctacgcaaa aaacatgccc attattgatt atcactgtca cttagatcca aaagaaattt
     1621 acgaaaacaa caatttcagc aatctgacag aagcttggtt aggaggcgat cattacaaat
     1681 ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat cacaggaaat gcttctgatt
     1741 ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat tatcggaaat ccgctttaca
     1801 gttggtcaca tcttgaattg aagcgtttct tcggtattga cttgttgata aatgaagaaa
     1861 atgcagagaa aatttggaca caagcaaatg aaattttggc agctcctgat tttcgccgtc
     1921 gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac aacagatgat cccgcagacg
     1981 acttgcacta tcatcagttg cttgctcatg aagaaccaga tttgaaggtt ctaccttcat
     2041 tccgtccaga taaagcactc aatattgaaa aagaaggatt taatgcatgg gtcacactac
     2101 tggaagaagc agctgaccaa acgatcacca cctatcaaga actgatagaa gtcttagggc
     2161 agcggataga atacttccat caacatggat gtcgtgtttc cgaccacgct ttagacatct
     2221 tacgctataa agaagcggat gaaacagtat tagaagagat ttttcaaaaa gcacgagcta
     2281 acaaactatt atcttcagat gaaatcgatg cttatcgtac agaaacactg atacatttga
     2341 cccacttcta tcattctcat aattggacga tgcagctgca catccatgct tatcgtaact
     2401 gtaatacaca aatgttggaa aaattagggc ccgataccgg ctatgatgga atgaatgatc
     2461 tttctttgac tattcctctg caaaaattat tgaaccgtgc agaagaaacg gatcagttgc
     2521 caaagacgat cctttattca ttgaatccga atgactatcc tgcgatttta gctttgatgg
     2581 gctgtttcca aaaagaacag aatggaaaac tgcagttagg ttctggatgg tggtacaacg
     2641 atactcgagc tggtatgcgc gatcaattga cacaatttgc tgatggtagt gctttgggga
     2701 attttgtcgg catgctgaca gattcaagaa gtttcctgtc ctacactaga catgaatatt
     2761 tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa gcacctgaag
     2821 atgaacaact tttaggaagt ctagttcaag cgattagtta tacaaatgct aaaaattact
     2881 ttggcttttt tgcctaattg attaatagta acagtaacga atcaaaaaca ggatagtgaa
     2941 ataacggaat ccgtttatcc acgttttaga gaagaagacg gaccaaattt gctccgtctt
     3001 cttctcttgc tgttctgctt cattgcctat aaacctttat gaaagaagag ataacatgcc
     3061 tactatccat attgccggtg attctactgc ggcagaaaaa gatgaaaaca agcgtcccga
     3121 gacaggttgg ggagaaaaaa tctctgctta tttttcaccc gagatccaga tcaataacca
     3181 agcaaagaat ggacgcagca ccaaatcatt tatccaggaa ggaaggttag cccgtctagc
     3241 ttcacagttt cagccgaatg atttcttatt tgtacaattt ggacataacg accagaaaat
     3301 ccaagaagaa agaggtacgc ttcccttcgg tagctattta aaaaatctgc agatattttt
     3361 tgaatgcgca aaaaaagcga acgtccaaat ggtcttactg acatctgtga cgcgacgaga
     3421 ttacttagaa aacggaacat tgaatccaga tatacttgga gactatccaa aagcgatgcg
     3481 tgcatttgct gagaaacacc atattcctct tttagatgtg ttctcaagat cacaagaatt
     3541 gttccagacc ttctcaaaag aagagacaaa aaaattctac ctgcatctta tacctgatac
     3601 ttgtaaaaat tacccagaag gactaaaaga caatacccac tttagtccag aaggcgctga
     3661 taaagttgcc cgaataattg ttgaattgat aaaagaatcc cgtctgcctc ttgctaatta
     3721 tttacaaaaa ggagtttgag atgtttccat taaatttagg catccgtgcc cacgatttag
     3781 atgttcgaaa tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg tcccatatcc
     3841 aatttgcacc acaaaaagca tttcctgaac aacttaaatt aactgacatg acacttggga
     3901 gtgctgccta ttttggtgaa tacttccaaa aaaacaatat cgaactttcc attttaggat
     3961 gctatatcaa cctatccagc aaaaatcaag aagttcgtgc acaagcattg gcaactttca
     4021 aacagcaaat tgctctgtgc tctgcctatc aagccaagat gattgctacg gaaaccggta
     4081 gtgtctctgt cggttacaca aaagaaaatt ttactgaaga agcttatcag atcgccagaa
     4141 attctatcat tgaattagtc gctcatgcag aaaattttgg cgtaactgtt gctgttgaag
     4201 cagggatcaa tcatccaatc tataactatc agttagctaa acgtttaatc gatgaagtag
     4261 cttcaccaaa tttaaaaatc attttagatt gtgcgaattt gatgcataaa gaaaatcatg
     4321 ctgatcagga aaaagtgatc cgaggtgcct tggacaattt acatggttat attacagcgc
     4381 ttcatctcaa agactatatc atggaaaatg gaaagatccg catggtacct gttggcaagg
     4441 gctgtttaga tttccgcccg gtactgcatt atatcaaata tgagcgtcct ctaatgtacg
     4501 ccactcttga agctactcct gagatttatg ttcctgaagc aatagaacac ttgcagttta
     4561 tttaccaaga ttgttaagga gaagattatg gaggaacgaa tacagaaaag ttatcaaaaa
     4621 tatcatgcaa atgcttttca gacattgctg catctttatc agggaaatta ttggcgtttt
     4681 gccatgtcag gactcttttt tattatcaaa catcttccat cttggttaat gccgatcgct
     4741 attgccaatg taatcaatgc agcttcatcg aaagattctt ctcagttgca ggccatttat
     4801 ttcaacgctt ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa ttattttcat
     4861 gtaaaatacc atagcacttc gatccgtcaa gtagaagctg gactaagagc gacccttatc
     4921 aagaaaatcc aagaactatc tattccttat caaaaagaat tgcaatctgg acgaatccag
     4981 tcaaagatca tccgtgatgt agaagctatc caaacgttgt cctctcaagt atttgtcagc
     5041 ggattaaata tcgctgtcaa tttattcgta gctttaggta tcacgcttta taaaagtcct
     5101 gttgttttca tgtttttctt attgactgtt cctctcgccg cattcgttac tctctttttc
     5161 cgtaaaagga tcagacagtc caactcagac ttcaggcaag aaatggaaga gacttctgca
     5221 aaagtgatcg aagtgatcga attagcaccg attgctcgag ctcatgggtt agaaaaacac
     5281 gaaacaactc gcttaaataa gcattttact caaatctatg aaaaaggatt ccacttagat
     5341 atcctgcaat ctgttttcgg atcagtcagc tgggctagtt ttcagatatt tcagctgatt
     5401 tgtttgattt tcactgggat attagcttta caagggcgca tccaacccgg cgatgtcgtg
     5461 atgtatcaga cgtatttcac gacgatcgtc aattccgttt ctggaatgat cacgctgatt
     5521 cctacgattt caaaaggcat ggaatctatc acttctattg gcgaactgtt gattgctggc
     5581 gatgtagaaa cttatcacgg taagaaaaaa atccaacatt tggatggaaa ttttcgtttt
     5641 gaggatgttt cttttcttta tccggactca ctcaaaccga ttatcgatca tttttctcta
     5701 gaggtgaaag ctggagaaac aattgctttt gttgggccat ctggctctgg aaaatctaca
     5761 ctccttaact tattgattgg atttatccag ccgacttcag gaaaaatttt tctagatgat
     5821 cagcctttgg atgaactgga tatgcgaagt gtgcgaaatt atttagccgt tgttcctcaa
     5881 accactttgt tgttctccgc ttcgatcaaa gagaacatca cttatggctt gaaaaacgta
     5941 tcaaaagaac gtttagatga agtcatcgaa gctgcgcaac tttcttcact gattgaccag
     6001 cttcctgatg gtctggatac cttagtaggc gaacatggca acaagctgtc tggtggccaa
     6061 aagcaacgta tctccattgc acgagcgttg atacgtcagc caaaaatcat tttgctagat
     6121 gaagcaacct cagctttaga taaccagtca gagaaaaaaa tccagcaagc cctgaattac
     6181 ctgacagaaa cacccactac ttttattgtg gctcatcggc tttcaacgat caaagaggca
     6241 gataaaatcg ttgtgatcga tgaaggaaaa atcgttgaaa taggtactta cgaaaaactg
     6301 ttagctcaaa aaggctattt ctaccgcttg aaaaatgaat gacaaaaa
//
LOCUS       DACDVT010000107         6315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDVT010000107 DACDVT010000000
VERSION     DACDVT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.109"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..556
                     /locus_tag="IWL52_002438"
     CDS             <1..556
                     /locus_tag="IWL52_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9778953.1"
                     /translation="SVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKVDSGLFGTGIK
                     ATPLESAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKSDKEGHFEITGL
                     TEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASVPMTGSNGFQT
                     YVLISCLLLGAGALSAVVYFKKKA"
     gene            571..1323
                     /locus_tag="IWL52_002439"
     CDS             571..1323
                     /locus_tag="IWL52_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9778954.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            1339..2100
                     /locus_tag="IWL52_002440"
     CDS             1339..2100
                     /locus_tag="IWL52_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778955.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            2113..2373
                     /locus_tag="IWL52_002441"
     CDS             2113..2373
                     /locus_tag="IWL52_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778956.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            2370..4460
                     /locus_tag="IWL52_002442"
     CDS             2370..4460
                     /locus_tag="IWL52_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9778957.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            4487..4951
                     /locus_tag="IWL52_002443"
     CDS             4487..4951
                     /locus_tag="IWL52_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778958.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            4948..5190
                     /locus_tag="IWL52_002444"
     CDS             4948..5190
                     /locus_tag="IWL52_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778959.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            5208..5510
                     /locus_tag="IWL52_002445"
     CDS             5208..5510
                     /locus_tag="IWL52_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778960.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            5580..>6315
                     /locus_tag="IWL52_002446"
     CDS             5580..>6315
                     /locus_tag="IWL52_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778961.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHEGRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYSTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNE"
BASE COUNT         2333 a          993 c         1282 g         1707 t
ORIGIN      
        1 tagcgtggat ttcgtttata acaacaatcc atttaatcag gaagaacacc acgaaaaaac
       61 gaaagctgac gttgtgactt atggtgcaaa attcttaaaa gtcgatagcg gactatttgg
      121 aacaggaatc aaagcaacac cgcttgaaag cgcagaattt gctgctaaaa acgcagaagg
      181 aaaatattat ggtggattag ttgatacgga taaaaacggt gtcaaagaag cagtatgggt
      241 agacgatgta gcaaatgcag cgatcctaaa atcagataaa gaaggtcact ttgaaatcac
      301 tggtttaact gaaggtgaat attcactgga agaaacaaaa gcgcctgaaa actatcaaaa
      361 attaacgaaa gagatctctt tcaaagtaga caaagattca tacaaagaag aaaaccgaat
      421 tacgatcaaa aacaatcaaa aagcttccgt accaatgacc ggtagcaacg gttttcaaac
      481 gtatgtatta atctcttgct tgttgctagg agcaggggca ctttctgcag ttgtttactt
      541 taagaagaaa gcctagaagg atcaaaacca atgattatca ggaagcctaa gaaaaagcga
      601 atcatgggta aatggatcat tgctttctgg ttactttcag ctgtaggtgt cttacttttg
      661 atgcctgctg aagcgagcgt ggcgaaatac caacaaaatc agcagattgc cgcaatcgat
      721 cgaacaggaa cggcagcaga aacggattct tctttggatg ttgctaagat tgagttaggc
      781 gatcctgttg gtattcttac aattccaagt atttctttaa aactaccaat ttatgacgga
      841 acgtccgaca aaattttaga aaatggtgta ggtattacgg aaggaactgg tgatattaca
      901 ggaggaaacg gtaaaaatcc tttaatcgca ggtcatagtg gactttataa agataatctt
      961 tttgatgatt taccttcagt aaaaaaggga gaaaagtttt atatcaaagt tgatggtgag
     1021 cagcacgctt atcaaatcga tcggattgag gaagtgcaaa aagacgaact acaaagaaac
     1081 tttgtcactt acttagaacc aaatcccaat gaagatcgag taacgctaat gacatgtacg
     1141 cctaagggga tcaacacaca ccgttttcta gtgtatggga agcgagtaac ctttacaaaa
     1201 tcagaattaa aagatgaaga aaataagaaa caaaaattat cttggacatg gcttttagga
     1261 agtactgttt ttctttcagt catgatcatt ggttcgctat ttgtttataa aaagaaaaaa
     1321 taaaggaggg taaccaacat gagaaataga aaatggctat ttgtttgtgc agccgctctt
     1381 ttttctttca ctgcctacac acagttgacg tttgcagaca gacaagaaag cacggatagc
     1441 acagaacaaa caatccaaac gtcagatagc agcacgattc aaagcagcga tgaaaccgtc
     1501 gattcgtcaa cagttgattc ttcagaagaa ccgaaaattg ctgaagacga tcaaatagtc
     1561 aaacagacca ttcacgttca aaaaattatt tcttccgaac aattagacaa ccaggggcaa
     1621 aaacttgaaa aacaagaagg agtaaatggc gcgaagtttt ctgtttatga tgtatcggac
     1681 attttacaaa agatggatgt taaggatctc actactgacc aaattgaatc ccaattaaaa
     1741 gatcgagtga aaaaactatt cagtgatcaa ttgaagctag tttctaatgg aaaaacaaag
     1801 acaattgatc aacaaacagg tgtctttgaa ttctcgatag aagtacaggc aaatcagaaa
     1861 caagcttatt atattgtcaa tgaatcaagt cctgaaaaca tttctaatag cgaggatatt
     1921 ttgctattaa cacctgtttc tgataaaaac ggtgaattct taaaggatgt gtggatctat
     1981 cctaaatcag aagcttcgca acctaaagaa gaagtaaaaa aaatcgtttc tactggcgta
     2041 aagaaaaact tctttgagaa ttgttgggat tttgttactc acttgttttc tagagattaa
     2101 ggaagaaata aaatgaaact gatcaaatta ttattattca atttttcttg tttaaccgca
     2161 gtctttttgt actgcggttt attattttct aaactatcta ttttgccaag ctaccaacta
     2221 tacctaatga ggattttaaa cataccagct gcttccatgc cgctagatat tatggaaaag
     2281 ctcattagga atccattatt tactacgatc aatttgatcg ttattgtcgg aatttcacta
     2341 gtcatttata aaaaagaggg gatgaaggga tgaattttaa acactcgtgg acaagacggt
     2401 taaaatatgt ttttcccata atgatgatgt taggtacatt gtttgggtta aaaacagcca
     2461 atgtctttgc tgaaaaagtt attgtcgatc cgtcaaatcc aatccagaac gtaaagaata
     2521 attttattat tccaaaagat gttcctaatg caaaaacaaa tattactgta aacggagcgt
     2581 caaaagttta caaatatcat gttgatgcca acaaaggtgg ttttacagac gactacgtag
     2641 gattagtgga gggaaacaaa aatatacgct atccttcttt ccaaaaagaa tacggtgaaa
     2701 caaaccttgt tttagaaggc gtaacaacca acaccaaagt caatattgac tatggaaaaa
     2761 tcggtacgta taacgggaaa aaagtcaata tcaagctagt tctatctaat attcacttat
     2821 actcggatac gttgccgtgg aatctactag ataacaacta tacaaaaact cattttcgag
     2881 acgatggcta taaaaatacg aatggtgcaa tgagtaaatc taaaaaaaga acagtacttt
     2941 ggatctcaga caatcttttc tcaggaattg tttaccattc aacacaaatg aatgtgcagc
     3001 tagtagccac atatgaggac ggtagccccg ttcaattttc aggagataca tttatcagtt
     3061 ttaactcttt gaaccctgca ggaggaaaaa ccacggatct taaaggagaa tatgcccact
     3121 atgacaaaat gaatacgaca gattggtatg tcagaaaaga tacggttctt tcagaattta
     3181 aatctttcta caacaatctg aacgtggtag gtggccaccc aggaggaagc tctaagctga
     3241 cacaagcgga taatgatttc aataatttgc atgataaatt aggagatccg aagtttggtc
     3301 aaggtactgt gtcctttaaa atatctgaag caaatccgac gtttgtgatt ggttcttcta
     3361 atgttcaaac ctggtttact ttatcttctg cgacaatctt tagtgttgtt cctgatcaac
     3421 ctgaaaaaac aggggttgat aaaaatggaa ataacgtgaa cgataaaatg ttgcaagtag
     3481 gagacacgat tcaatatcga atcaaacaga aggtgaatcg attaggagtg gatttactag
     3541 cggtttacga tcgatttgag ttaatcgata atttaccaaa agaagtaaat tacgtcgatg
     3601 cacacgttga aagtggtaca aataaaaaat tcgatgtatc tggtgaagtg acctatgaca
     3661 aaacaaaaca ccaggtaaaa tatgcagcga aagctgatac attaaaaaat cgaatgaagt
     3721 ataacggcga aacttacgaa ttagtgatca atgtaaaagt caatgaattg gccaatcaaa
     3781 atagtgtagc caaaaatcag ggaacgtcga tcattaataa agtagaaaag gatacaaata
     3841 tcattaccgt ttatttccct aaaattccag tgaaagaagt acaacaaaat ggaaaagatg
     3901 taaacggacg aaatgacggt aagaagggta cgccgacagc tcctttaaac gcaggatctg
     3961 aagtacaata tctagtcaca caaaaatggc acacaaaagg cgtagacgct gcgtcagatc
     4021 actacaaaca atttagtatt caagatccaa ttgaagctcg tttgacatat aaagaaggtt
     4081 ctgcgcaagt aatagataag agtaaaggaa aagatattac ttctgaaggc acactaacct
     4141 atgatagcaa ttcaagaacg ctgaagtggg aagcttccgc tgacttctta agtaacaatc
     4201 ttttagatgg acgagaaatt cagctgatat ttacagctaa aactccatta caatcagaaa
     4261 aaaatattga taaccaagcc gtagtagcag tagataatgt ttctaataaa acaaatgttg
     4321 tgacaattgg ggttgatcct aacttgccac aggtcattgt tcctaaaaca ggttctacac
     4381 atttagtaac gatttcagcg gtcagcttac tattacttgt gttagcaact tttagttatg
     4441 cggttctgag atacatttaa aataaaaaaa cgaaagaatg aataaaatga aaaagttaat
     4501 aatagtagtt acttccttga ttgccatgat tgttatttta gtcggatgta gtaatgaaaa
     4561 aaataaacaa actaatcaag ataatggcgt attgaaatca ggaactatgt ggaaagaaga
     4621 agtaggtggt cttgtttata atttgaaaat tatagacgag actacctggg aatactctga
     4681 gagtgtatgg catccagatc ctgtacaaat aacagtcaag aggcaaaaag actataaagg
     4741 tttagaaaga tacaaaatag tagattcagc tggtgttagg gaatttataa acaaatctga
     4801 ttctttattt attgttgttc cctatgagaa gaatggcgta aaaaaaataa tttttcttgg
     4861 atctagcaag gatgaaaagc gaacgaaaga gaaattgatt cacgatggat cacaatccaa
     4921 taaatataag ttacaaaaga ctagcgagtg attgctagtc tttttatttg tcctgaagga
     4981 ggaatcacag tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa aatcttagaa
     5041 cgccgcggtg tatctgaaac acagtggaaa caagaagtcc ttgaaaaagc acaatttgac
     5101 ctattacggg gaaaagacaa ggacttagaa ggatatatct atcaacgtga acgtgaaaag
     5161 cttgttttcg acgaaattat taaacactaa aaggagcgaa caattttatg tttgaatcaa
     5221 ttacaatgaa ttttttaaca caaccgaaag tagtagctgc ggttgatatt tttagagcat
     5281 tacaaactgc cgcaactagt ttaaaaaaca atctgatgat ttttgctgct gctgcggccg
     5341 ttgcttccat tgttattggg ggtcttttct tcctatttgg acgaagagga gctgaaaatg
     5401 ggaagatgca cgtaggctat gtattgatgg gggtaggagt tattttagca gcagcttcta
     5461 ttgtggtttc gttatttgga ttgtttggac aaacacctca aggattataa gaaagtaaaa
     5521 aagtaggagt aagaagtagt cgatactttt tactcctttt ttatgaaagg agaatactca
     5581 tgtactttac gattgcaggc aataccaaaa cagataaaga gttaataaaa aaagcaacga
     5641 aggaagtagg aaagcgtact tcttttttac cgaaaacaat taaatattct gtcattgaaa
     5701 cggaagaaca gctacatgtg ttagggaaag tactggaaca cgaaggtcga tcttctgtta
     5761 agttgactct cacgttacat gacacagaag gaaaaataga gaaagctggt acagaatacg
     5821 tttttggccc actggattat caaatttcag aaggtttcta ttctacattg atggatttaa
     5881 tccgccttga tgtgtttaaa gaaacggatg aaactatgag tgtggaagag tcttatcgaa
     5941 acgaaaaact tttacaagtt ttagaagaag agttagagag cgaccaaaga aaagtgaatc
     6001 aagggagtga agtcgaagta aatgaagaag taccatggga acgaccaata tttccaaaga
     6061 aggagtcaaa agaaaaagag acagtctatg ttgacgaaca agctgatatt ccgactcaga
     6121 aatcgatatt tgagaaagag aacgatgtga tgggtcagtt ggaggtagat ccctttgaag
     6181 aaacaatgga ggatgaagag acggccgaag tagaagttga tccagttgaa gctaatgaag
     6241 agtcaacatg gactgaaggg gattctcagg aatatattga accacttatc agtgaagaag
     6301 tagaaaaaaa cgaaa
//
LOCUS       DACDVT010000108         6282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDVT010000108 DACDVT010000000
VERSION     DACDVT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.110"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..219)
                     /locus_tag="IWL52_002447"
     CDS             complement(<1..219)
                     /locus_tag="IWL52_002447"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9778962.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="IWL52_002448"
     CDS             complement(330..584)
                     /locus_tag="IWL52_002448"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9778963.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="IWL52_002449"
     CDS             complement(777..1052)
                     /locus_tag="IWL52_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9778964.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="IWL52_002450"
     CDS             complement(1024..1977)
                     /locus_tag="IWL52_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9778965.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="IWL52_002451"
     CDS             2589..4082
                     /locus_tag="IWL52_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9778966.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..4513
                     /locus_tag="IWL52_002452"
     CDS             4217..4513
                     /locus_tag="IWL52_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9778967.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4537..5496)
                     /locus_tag="IWL52_002453"
     CDS             complement(4537..5496)
                     /locus_tag="IWL52_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9778968.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5679..>6282
                     /locus_tag="IWL52_002454"
     CDS             5679..>6282
                     /locus_tag="IWL52_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778969.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT         2029 a         1049 c         1110 g         2094 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
     4501 gtatatccag tagtagtttt tatttactct taattgtcaa attaagttag acaaatcatc
     4561 aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg
     4621 aatattattt cgtttcgatg cgacagattg aataaaagat tcatctactg aattgaaatc
     4681 catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct
     4741 ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat tactgatctg
     4801 tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata gatttttcgg
     4861 tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc taccacacgg
     4921 tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag cacttttatg
     4981 tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga attggctata
     5041 atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc tttcttgatg
     5101 tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata gccccttttt
     5161 gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac gaccaacaat
     5221 cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt gttcctcagg
     5281 taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt gatagtactc
     5341 taaggctgat tttccttgct tgaagaacag gtatacttta tggatcgttt gtcttgaacg
     5401 attcaagcaa tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag actctatcat
     5461 tacaagttca tccgttgtaa gatgggtata ggtcatttgt actcactcct tataattctt
     5521 tggacggaac tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta
     5581 caatcggcgt acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag
     5641 gaaaacgtta aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg
     5701 aacacacctg gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat
     5761 tatggccatt aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt
     5821 actcgattgg agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag
     5881 catcagacat ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta
     5941 ctcaattagt catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc
     6001 tattcgattt aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat
     6061 ctggaagaac aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg
     6121 gacacatgta tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga
     6181 aacttattaa acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg
     6241 gttatgagta caatgttgat tttggaagat atgacgaatt ga
//
LOCUS       DACDVT010000109         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDVT010000109 DACDVT010000000
VERSION     DACDVT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.111"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWL52_002455"
     CDS             725..811
                     /locus_tag="IWL52_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9778970.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWL52_002456"
     CDS             complement(932..1621)
                     /locus_tag="IWL52_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778971.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWL52_002457"
     CDS             complement(1628..1906)
                     /locus_tag="IWL52_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778972.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWL52_002458"
     CDS             complement(1866..2780)
                     /locus_tag="IWL52_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9778973.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWL52_002459"
     CDS             complement(2762..3142)
                     /locus_tag="IWL52_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9778974.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWL52_002460"
     CDS             complement(3592..3879)
                     /locus_tag="IWL52_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9778975.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWL52_002461"
     CDS             complement(3899..4123)
                     /locus_tag="IWL52_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9778976.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWL52_002462"
     CDS             complement(4763..5296)
                     /locus_tag="IWL52_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9778977.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWL52_002463"
     CDS             complement(5317..6159)
                     /locus_tag="IWL52_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9778978.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1041 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtacactatg ccaagtagcc
     4321 cttgtagcaa tcgatacagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDVT010000110         5933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDVT010000110 DACDVT010000000
VERSION     DACDVT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.112"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..742
                     /locus_tag="IWL52_002464"
     CDS             <1..742
                     /locus_tag="IWL52_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9778979.1"
                     /translation="IYSLLSEKEQENFDEYAYFVNTAIDRQSLGKVLGGKMISVVEPY
                     YEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAAHAAVAYLGALKGYKTIQ
                     EAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKTLNRHGNQKLSDEITRVG
                     SSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYRNLADPEAEQLRRMIVND
                     GLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            754..1185
                     /locus_tag="IWL52_002465"
     CDS             754..1185
                     /locus_tag="IWL52_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9778980.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            1199..2644
                     /locus_tag="IWL52_002466"
     CDS             1199..2644
                     /locus_tag="IWL52_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAP9778981.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            2930..4555
                     /locus_tag="IWL52_002467"
     CDS             2930..4555
                     /locus_tag="IWL52_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9778982.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            5187..5540
                     /locus_tag="IWL52_002468"
     CDS             5187..5540
                     /locus_tag="IWL52_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778983.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         2071 a          818 c         1159 g         1885 t
ORIGIN      
        1 aatttattct ttgctgtcag aaaaagaaca agagaatttt gatgaatatg catattttgt
       61 gaatacggca atcgatcgac aatctttagg aaaagttctt ggaggaaaaa tgatctctgt
      121 tgtcgaacct tattatgaat ggataatgga tcgacagcag ttagatccaa ctacatcttt
      181 tgatatgaaa aatgtaaatt tggttgatga tatggagcct tacattgagc gaaagcttta
      241 tatcgttaat gctgctcatg cggcagtagc ttatttaggc gcactgaaag gatataagac
      301 gattcaagaa gcaatccaaa atatggagat tttcaatcta gtcaagcaat ttttaatgga
      361 gaatatagag tactttgtac aaaaatatca tttttctaga gaagaattaa catcttttgt
      421 ggaaaaaaca ttaaaccgtc atgggaatca gaaactctcc gatgaaatta ctcgtgtagg
      481 aagttcccct attcgaaaat taggccctaa tgatcgttta attgctccca tagtaaaact
      541 agattcatat catttacctt acgatgcagg aataaaagta attgctgcgg gatatcatta
      601 tcggaatctt gctgatccag aagctgaaca actacgtcga atgattgtaa atgatgggtt
      661 gaaagcaacg attaaaaaga tttctcgttt ggaaggtaag ctgctgaatg aagttgtcgc
      721 tgcttatgag tcccagattt agaggaggag aatatgctaa aagaacaatt gtcaggaaat
      781 acaatgttta tggatgcgat cgattcatgg caagaggcaa tccgagtaag tgcgcgtcct
      841 ttgataggaa agggaataat agaggaaagc tatgtacaag cgatgataca aaatgtgttg
      901 gataacggaa attatattat attactgcca ctagtagcaa tgcctcatgc gcgtccagaa
      961 tttgggagca aaggggtcgg gatgagcttt ttatgtttag ataagccagt catgttccca
     1021 agtgaagaac cagtaaaatt cttctttacc ttttcttctg attcgccaaa gggacattta
     1081 gacttaatgg ccaatcttgg agaattgctg agtgatgaaa aaatgtatca aaaattattc
     1141 gatgtgaaga cagaaaaagc attgttagat ttgataaaga agtaaaggag agaagaaaat
     1201 ggaggtagca aacaaaaaaa aacgagtctc catgcgagct aaagttcaag cgattggcgg
     1261 tttcttaaca aatatggtat tgccaaatat aggcgcattt attgcatggg gaattgttac
     1321 tgcattattc attccaactg ggtggttccc aaatgaatct ctgaatgaac tcgtaagtcc
     1381 gacagtcagc tatctattgc cgttactact tgcctatact ggtggtcgga tggttggtgg
     1441 tacacgtgga gctgttttag gtacaatcgg aacaataggt ttgattattg gtgctgacat
     1501 accaatgttc atgggagcaa tgattatggg gccacttggt gggtggatga tgaaaaaaac
     1561 agatgatttt ttagatggga aagttcctgc tggttttgaa atggtcgtca ataatttttc
     1621 tataggaatt attggtttca tactgatgtg cctttcttat ctggtagttg gaccaataat
     1681 ccaatcagct aatcaatttg taacgagtgc aattgaagca ttagtttcaa ctggtttttt
     1741 accgatactg tccttaatca acgaaccggc aaaagtctta tttttaaata atgttattga
     1801 ccaaggtgtt tattatccat taggtatgca acaaacatta gaggcaggca gatcaattta
     1861 tttcatggta gcatcaaatc ctggccctgg cttaggacta ttgttagctt attctttatt
     1921 tggtaaaaag gcagcaaaac agacagcacc aggtgcaatc atcatccatt ttttcggcgg
     1981 aatacatgag atttattttc catatgtatt aatgaaacca ctcactatat tatctatgat
     2041 tgctggtgga atgacaggaa tcatcacttt tgaattattt gatgcgggcc tggtagcagg
     2101 tccaagtcca ggttctattt tttcttattt agctctaacg cctcggggaa attttttggg
     2161 tgtcatggcg ggagtggtca cagcaacact ggtttccttt tttgtaacat cagtaatttt
     2221 aaaagcagat aaatcaacag atgaagacag aaattttgaa ctatcagttc aaaaatcgaa
     2281 aacgatgaaa tcagaaggaa gaaaattatt aaaagaagaa aatgctagag aaatatgtac
     2341 cgagaaaaaa gagatgaata aaatttcctt tgcttgtgat gcaggaatgg gaagcagcgc
     2401 aatgggcgca acaatattca agaaaaaatt gcaaaaagca ggtattactg atgttgagat
     2461 aaaaaattat cgaattgaag aggtaccaga agatagtgat ttggtgatcg tacatcaaga
     2521 tttggaggaa cggactagaa aagcacatcc taacatgaaa attgtgacat taaagaatta
     2581 tttgcaagat ccagtaatca atggattagc agaagaaatc attggattaa aaacagaaga
     2641 ctaatttatg ggaatttttg attaaccttt attaaaatta ttttgcctaa tataaaaagg
     2701 ttgtgacatg gcttgtgtcg caaccttttt ttgtcgcaca aataattaat cggatatttt
     2761 atgttcatat tttttgtgat caacgagaac tgatggggca aagatacatt ttagatactt
     2821 ttaactgtta atttacaaaa caaggtgaaa cgaatagaaa aatgaaaaaa tcgatacgga
     2881 agaatatgtt tgattaaaac atgcatgaag agaaggaggt tgatgataca tgaaaaaaat
     2941 atattcaaag gaaatcgatt gttttagagg cattgctgtt ttagcagtga tattttatca
     3001 tttgccaaat agtttgctgc caggcggatt gcaaggagta acgatctttt ttgtggtttc
     3061 tggtttttta atggctaaaa atacgataag agatgaaaaa aacagacaat tcagtttttt
     3121 acatttttac tataagcgta tcaaacgtat ttatccattt ttactgtttt ctttggtcgt
     3181 attcattttc atactaggga ttagcaacat tcgattgata ggaaacattc gtagtgagct
     3241 acccagtttg atttttggct ataacaattg gtggcagctg aatcaaggag tatcttattt
     3301 tgactcttac tttaatgctt ctttatttaa acattattgg tcgctttcag ttgaactaca
     3361 attctatttg atatggccat ttttgtttat tgtcattaag cgtatgagaa gaaaacaagt
     3421 gttttactta atctatacat tgatttttgt ttctattctt tttagtttgt ttttaccttc
     3481 aactaaggca tattatcata cgatagctaa actttttcca tttttattag gagtttgggg
     3541 gtattttaat cgaataacta ttggccgttt ctttgaacaa aacactttta gtaaaatctg
     3601 gcttttatta ctagctagtc tttgtctgat tctgttcccg atttttcctt atacactaaa
     3661 tgaactactg atcagtattt gttttgctct attgttagcc agtgtcgatg atatgaacat
     3721 tgcaaaaaag accaaaatcg ctagtacaaa actttctttt gtaggaaaaa tcagttatga
     3781 actttatttg gtgcatttcc cagtattgat tgctctgaca aatctattca aacatcacca
     3841 ttggaaatat ttggatatcg caggtctatt cactgtgttt ttatgtacat tactcatgat
     3901 tacttgcttt agaaaaggag tgcaaaagat gagatcaccc aaaatttgga cagctatggc
     3961 actaatgata atcggagttt ctgtactggt attacgtgct cctgccagtc gactgaccgc
     4021 tgaccaaaat cagttgcaaa gtttattaaa agaaaactct agtaaagcaa atgcaggaga
     4081 aaatctgttg tcacaagaga agaaaatatt atttatcggt gattctgtta tgctgggggc
     4141 gtatcaggaa ctaaaggaga catttaaaaa agaagcattg gtagatgcaa aagaatcaag
     4201 acaaatcact gcgttaccag aaatcttaaa ggagcataaa gatttagttt cttaccaaca
     4261 aatagtgatc gggttaggaa ccaatggtgt gataacagat gaagctatag aacaaacaat
     4321 gtctttgctg aaagataaaa aagtatactg ggtaaatata aaagcaccaa ctggctggca
     4381 agatacgata aatgccacat tagctacatt gccaaatcag tattctaata taacgatgat
     4441 cgattggtat agtgagagca aaaatcatcc tgaatttttt tatgatgatg aaacccattt
     4501 gaatgaaagt ggacgtacag cttatgcaaa gtatattgct tcagttattc aataaggtag
     4561 tgattacgtg tgttaacctt actttttcct aataggtgaa tcaatttaaa taaaaaatac
     4621 ttcatatgat aaacggtgtt taagaggagg agctttttaa tattcgtggt aaaaaagaga
     4681 tgggcgaggt cgttaactga gagcttcttg gaaagaaatc gctttggatt tatcctcttt
     4741 gttatacaga acatgagcga gacgacaata ctaggatgta tgttctgatc gaagttttat
     4801 ggttacaagc agcaatcagc ttagataaaa ttaaggatcg taggaaccaa gtaaagaagg
     4861 ctgttagtaa agaaacaaac gatcaagtat gttttactct taagaagatg cagtgaataa
     4921 ataatagtaa tataatgcta gaagggactg tgacaaaaga cgtgtcacag ccttttttgt
     4981 tattttaatt atcgtccagt ctttttttat aaatgattgc ttaagcatta aatattaaaa
     5041 aatatagagt tcaaaattac tgcacaatta gatgaaaaat ttgaacaaca aaaagtgtat
     5101 ttttatacta taatagcttt tgaatttagt tactctattc aaaagttatg ctatggacaa
     5161 gaaaaagaat aaatagaggt gagaaaatga atagtttcaa gaaaaaattt gtgagtagat
     5221 ttttaaatat gaaaaatttt tttgaaaaaa agggagatta tgatgcatat aattatgtat
     5281 taaatctaga aaagcaaaca tattatacac tacgtaaaaa cgatgataat gacttttgga
     5341 ctatgttacc taaaatgctt tcgattgact ctaagctttc ccttttagag gaatttatta
     5401 atttagacat tgaagaattt tgcgggggaa aagagttaat taatatggta gagagtgatt
     5461 atcataccta ttataaagaa agttgtggat acaaagtgga tgagcaatgt ccacaatcaa
     5521 ttatttttta cattgagtaa tatgaactat taagcaggaa agcaactaga agctactgat
     5581 ttagtattta caaatataaa ctatattgct aacaattatt ttctagaagg aataaaagaa
     5641 ctaagaatca agacgttatc ttgattctta gttcttttaa catggaaaag atggataatg
     5701 cactgttaat tcatgatgca tcatcagaaa agttggaagg agaagataga aatcattaaa
     5761 tgatttctac agatggtaaa tgaaaaaaca aaatattagc aaaaatttgc ctaaatagat
     5821 agtaacactt tctaaattaa atacaagcgc attcataaaa tgtttataca aatatctatg
     5881 tgataaaaat tggatatgtt cacaataaat aggaattaaa ataacgaatt tac
//
LOCUS       DACDVT010000111         5931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDVT010000111 DACDVT010000000
VERSION     DACDVT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.113"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(48..1553)
                     /locus_tag="IWL52_002469"
     CDS             complement(48..1553)
                     /locus_tag="IWL52_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9778984.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1550..2236)
                     /locus_tag="IWL52_002470"
     CDS             complement(1550..2236)
                     /locus_tag="IWL52_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9778985.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2429..3850)
                     /gene="gndA"
                     /locus_tag="IWL52_002471"
     CDS             complement(2429..3850)
                     /gene="gndA"
                     /locus_tag="IWL52_002471"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9778986.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4020..4199)
                     /gene="rpmF"
                     /locus_tag="IWL52_002472"
     CDS             complement(4020..4199)
                     /gene="rpmF"
                     /locus_tag="IWL52_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9778987.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4257..4826)
                     /locus_tag="IWL52_002473"
     CDS             complement(4257..4826)
                     /locus_tag="IWL52_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9778988.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5012..5914
                     /locus_tag="IWL52_002474"
     CDS             5012..5914
                     /locus_tag="IWL52_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9778989.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT         1683 a         1263 c         1011 g         1974 t
ORIGIN      
        1 accttattct ttgttggata tataaaaaaa caagtaaagc acatgcttta cttgtcttct
       61 gcctttgcga taggaataaa gattctaaaa attgtccctt gccctactac actttctgct
      121 aagatacgtc ctttatagtt ttcaaccagt tgtttagcga tagacaaccc cagaccattt
      181 ccacccttcg ttctagctct tgctttatcc actcgataaa aacgattgaa gatcttatta
      241 aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg cgatctcaaa ttcattcaga
      301 gtggaagaaa tagagatatg gacttctttt cgtgtagttg aatactttac tgcgttatcc
      361 aatatgataa tgattaattg ttcaaagtga ttacgataaa tttgcagggt cacttctttt
      421 gtcaaatcat catctaaagt gatcacatag tctggatata atatcttgaa attattataa
      481 acttgatagg tgacttcttt tcctttagtt gtttcattcg cataatgaac atctacttgt
      541 tctgctcttg ataagtctag catctcttga actaagcttt tcatacggct aatttcttgc
      601 atgctggcgc ttaatgattc ttctaatatc tcaggatcat ctttccccca acgatttagc
      661 agactaagat gcccttcaat aattgcaaca ggtgtcctta actcatgaga tacgtcttcc
      721 acgaattgct cttgttgttc aatatacaaa cgcatacggt caagcatctc attgaatatt
      781 tcagctaaat ctgccaattc atcatttgtg tcgatttcag gcatgtggat atctgattgc
      841 ggatctttac gtatagtatc cattgtatct ctgagtacct tgagtggttt taagaaataa
      901 gaagataaaa agaaacctaa aatactactt aagatcaaag aaacaacttc taaaacaata
      961 agagtcaata atagcttatt tcgaatatca tagaaagaag ataattcata gaagctttgc
     1021 gcatagccga tcttttctct tgtaccttta gaatatatag gctggataga taaaaagcca
     1081 gtttttcctt ctagtgtaac gatcgttgga actttacgtg tagtctgaat caggtttcga
     1141 ctgttttcat gggttttaaa aatcaagttc tcgtccaaat cataaacata aagatccatt
     1201 gatggctgcc ctagctcagc tataaaggaa tcgatcttca ttaagtttcc ttcgaccagt
     1261 gaagtatgat ctacgacctc atctccgtca tttgtcgctt tcgtcagatt acgatacgta
     1321 tttactaatg tcagttcttc atccgagtta gccaaacgag aagctacttc tgaaatcgta
     1381 cgctctacat tgtttctttc ttttgccaca atcagattga tagatgattt ataggtaatg
     1441 acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac tcgcaaaggc ccacttgatt
     1501 gtcaaggagg gccctttcag ttctcttttt tccgattttt tcggctttat cacgagcgca
     1561 tcacataacc tgttccgcgt acagtttgga tatagctttc ctccccggga acgtcaattt
     1621 tgttgcgaag ataacgaata tatacgtcca ctacgtttgt ttcaacttct gtttcgtatc
     1681 cccacacttt atttagtaaa acgtcacgtg ctaataccac gttcacgttt tccattaaag
     1741 taagcagcaa ctcgtattct ctttttgtta gttcaattac ttcatttccg cgacgtacga
     1801 cacggttttc tttttcgata gtcaaatcgc gataagtaat agtagtttgt ttagccacat
     1861 ttttatcgcc ttcaatatcg attcgacgca acaatgcacg aagacgtgct aataattctt
     1921 cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg gtcaagtcca gatacacgat
     1981 cgatcacaga atctcttgca gtcatcataa tgattggtgt atttttgact tggcgtacac
     2041 gtctgcagac ttctaaaccg ttcaattctg ggagcatcag atccaacaat atggcatccc
     2101 actcattatt taatgccgca tctaaaccag ttcggccgtt gtagtggact tctgcgttat
     2161 acccttcatg cttcaactct agctcgacaa atcgtgccaa gttcttttca tcttcaatga
     2221 ttagaatatt gctcatttat tattttatcc tttctattct actggctttt taaaaactct
     2281 atcattatac cgataatatg cttaaaaatc tatagtcatt ttatcatgga tagcaatttt
     2341 aaaaaagagc tgaagtctac tcataacaat cgacttcagc tctttcgtcc tctttttatt
     2401 ttctcatttc ctaacagccg aaagactatt attcttcatg gtaccatgag taatggaaga
     2461 ttccttcttt gtcgacacgt tcataggtat gtgcgccaaa gtaatcgcgt tgggcttgga
     2521 ttatatttgc aggcagacgt tctgctctgt atgaatcgaa gtaagcgatg gctgatgaga
     2581 aagtcggtac aggtacgcca gcttggacag ccaaggcaac gacgtctctg actgattgct
     2641 gatatttttt cgtgatttca acgaagtaat catctagcaa taaattttcg atctcaggat
     2701 ttttgtcata ggcatccgta attttttgca agaatcgagc tcggatgata catccagcgc
     2761 gccagatttt tgcgatttca ccaaatggca agtcccaatc aaattctttt gaagcgacac
     2821 gtaattgagc aaatccttgg gcataactca tgattttact gaaatacaac gcttctcgga
     2881 ttttttcgac caattctttt ttgtctcccg tgaactcaaa atcgttcgta cgagataaaa
     2941 tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat atagcgggca aatacagatt
     3001 cagtgatcaa tggaagtggc acacctaagt caagcgcgct ttggcttgtc catttacctg
     3061 ttcctttatt cccagctgca tcaaggatca catctactac tggtttacct gttccttcat
     3121 cgtctttacg tgtcagaatg tcagcagtga tttctatcaa atagctgtct aattcgcctt
     3181 gattccattc tttaaagata tcagccattt cttctactga aaggtttaaa atattcttca
     3241 ttaagtcata agattcagca atcagttgca tatctccata ctcaataccg ttgtgtacca
     3301 ttttcacata atgtccggct ccatttgggc caatatatgt gacacatggt gcttcgtctt
     3361 ctgcttttgc agaaatttgc tctaagattg gtgcaactag cgcataggct tctttttgtc
     3421 caccaggcat gattgaaggt ccttttaaag ctccttcttc accaccggaa acgcctgtac
     3481 cgataaagtt gatgccagag ttggctaact cttcattacg acgcatcgta tctttaaaga
     3541 atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag gtgaggaagc aactcttgga
     3601 tcgttgcatc tgttgccggt cctgctttga ccattagcaa gatccgacgt ggtttctcga
     3661 tagattctac aaattcttcg attgtatatg ttgctttgaa atttttatct gggtgttctt
     3721 ctactacagc ggttgtttta gcccctgtac gattgaataa agccactgaa tagccgcggc
     3781 tttctatgtt caaggctaga tttttaccca taacggccat accgacaacg ccgatttgtt
     3841 gttttgacat atgaactcct cctacatacg ggatttctcc ctaatctctt acattatagc
     3901 ttaacacggc tacattgcaa actaaagaac aacatatttt ttatttaccg actacaaaaa
     3961 aataaacctc aaggctccat ggttagcctt gaggttttac attttctaat taaaatgtat
     4021 taagcttctt ttgacattac gtccttacca tcatagtaac cacaatttgc acatacgtga
     4081 tggctttttt tcatttcacc acagtttgga cattcgttta accctttgat tgtcaatttg
     4141 tagtgtgtac gacgttttgc ttttttagct tttgatgttc ttctagctgg tactgccatt
     4201 ttgctacacc tccttgtaaa gtcttgtatc taaattagac acaatatatt ccaatcttac
     4261 ttgttatcgt cgtcttttga tttagaatca aaaagttcag atagttttgc aagacgagga
     4321 tcaattttat tctcggcttc ttccattttt ctgcgctggt attcttcttc ggaaacaact
     4381 tcccaatcat tgccttttgg aagttctgtt gctgcttcct cttcttttgt caatacccgc
     4441 attggaatcg ccaataagat gttgtcttca atcgactctg ctaaattcaa taatggacct
     4501 tcaattagca aaatctcatc cgcagacatt tgttcatctc tactttggaa ttgttcttct
     4561 gtcatgaata cttcgtctac agaaaaatcc atgggcagtt caacaggttc tagtgaacgt
     4621 gttgaaggaa ctgtcaaagt cgtctgcaca gtgtaatgaa cgatatagcc tttcttatca
     4681 actgagacca aaccttcgac ttccactggc gcaacatcaa gtattaaatt gtctcgcttc
     4741 atgagagcgg ctttcaagtc gagcgtctca tgaaaagtta acggtgtctc ctggtatttt
     4801 ctcaattcca ataaagacca tttcattttt catcactcct aagcaacaaa agcaattata
     4861 cagggcaaaa agcatgttgt caatgaaatt ttcttgacac cttgcagttc ataaaaaaac
     4921 agaaaaatct ttattagaaa gcctctgttt gctatcatcc tcagaatcat ttacaataat
     4981 cattattcca ttaaaggagc ggatgatctt catgaacacg aaagatatcg aatactatgt
     5041 ccaaatcgtc aaacaaaaaa attttacgaa agtggctaat tatttccgtg tgagccaacc
     5101 tactattaca tatgcattac aacgtcttga aaaagagttt gacaccgtac ttattacaag
     5161 agatcgatcg catcacgaat taatcgtcac gccttctgga aagcagctgt ttctccatgc
     5221 acaaaccatt ctccaagaga tttctactgc aagagctgaa atcactcagt tgcaaaagaa
     5281 aaagcttcgt tttggcatgc ctcctattat cggcaatgaa tactttccta aactgtcaag
     5341 ttactttatc aagcatgaat tgatgaatca aattgagatt accgatggag gctctcggga
     5401 cttatacggg atgatccgac aagggaggat cgatttagct gtattaggct caacacagcc
     5461 aatcgtggat caatatttag aaacagagtt acttttggac aaacgtttca tgatcgtcgt
     5521 gccacctaca catcctcttt ccaagcagga tactgtctct ttcaaagata tagcagatga
     5581 acagtttgta ctattgaacg aacattatgt ccaccccgca gcattcacta agctttctaa
     5641 acaaacacat actcatccaa aagttgttta tcaaagcaat gacttgacga ttttaaagag
     5701 tatggtccga gaaaatgtgg gtatcggttt tttggcagag attgctgtcc atcctgaaga
     5761 tcatttagtt tgcctccctc tttcagatga agttcagcct cgcttcttga tttcattggc
     5821 aaaacgaaca cagcagatcg tcacgcctct tcaagaacaa gtagtccaag ttatccatga
     5881 attcgttaca gcacaaaaaa acagctcttc gtaaaagaag agctgttttt a
//
LOCUS       DACDVT010000112         5868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDVT010000112 DACDVT010000000
VERSION     DACDVT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.114"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..285)
                     /locus_tag="IWL52_002475"
     CDS             complement(<1..285)
                     /locus_tag="IWL52_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9778990.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYI"
     gene            complement(303..623)
                     /locus_tag="IWL52_002476"
     CDS             complement(303..623)
                     /locus_tag="IWL52_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9778991.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            922..1698
                     /locus_tag="IWL52_002477"
     CDS             922..1698
                     /locus_tag="IWL52_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9778992.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1713..1916
                     /locus_tag="IWL52_002478"
     CDS             1713..1916
                     /locus_tag="IWL52_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9778993.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1932..2252
                     /locus_tag="IWL52_002479"
     CDS             1932..2252
                     /locus_tag="IWL52_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9778994.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(2434..3216)
                     /locus_tag="IWL52_002480"
     CDS             complement(2434..3216)
                     /locus_tag="IWL52_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778995.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            3282..3974
                     /locus_tag="IWL52_002481"
     CDS             3282..3974
                     /locus_tag="IWL52_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9778996.1"
                     /translation="MLKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQKYADLF
                     WEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEKAIRKN
                     TAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVLGGKES
                     RAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQL
                     NRQQRLFGDENE"
     gene            4152..4493
                     /locus_tag="IWL52_002482"
     CDS             4152..4493
                     /locus_tag="IWL52_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778997.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            4486..5157
                     /locus_tag="IWL52_002483"
     CDS             4486..5157
                     /locus_tag="IWL52_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9778998.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5163..5849
                     /locus_tag="IWL52_002484"
     CDS             5163..5849
                     /locus_tag="IWL52_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9778999.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
BASE COUNT         2161 a          925 c         1095 g         1687 t
ORIGIN      
        1 aatatagata tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga
       61 gtgaaaagtt gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg
      121 tgcaagttca tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa
      181 aaaaatgatt ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac
      241 tttcaaattg tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc
      301 aactattcat tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta
      361 gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta
      421 ggggtttctc tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca
      481 atccgactta attcatctga attaggagta ttatttttcc actttgctag ataaccgtta
      541 gaatatccaa aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct
      601 tttattattt cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt
      661 ttagaaaaat acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg
      721 taaacagatt taacaactaa aaagacaaca aaaaacacta ttgatatgta aatgcagacc
      781 gccaagaaag ctttaaaatc aatgttttta tgtcttattt aattatgctc ttagtataga
      841 gtattactca gatactgtca attgaattta gaaaaaagtt gttaaatttg tttacgaata
      901 taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa
      961 gttcggacaa ttttagtaaa tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt
     1021 ttgggttatt caaacactaa agatgctttg tcgcggcatg tagatttgga agataagatg
     1081 gggtcgcgaa tcacgacctc aggtcaatca agagagatga caatcatcaa cgaatctggt
     1141 ttgtacagtt taatcttaaa atcaaaactt ccctctgcca aaaaatttaa acgttgggta
     1201 acaagtgaag tgttgccagc aattagaaaa catggaggtt atctaactcc agaaaaagta
     1261 gaagaagctt tgcttaatcc agatacaatc attcaattag caactcaact aaaagaagaa
     1321 agaactggaa gattaatcgc agaacaaaag attgcagagt acgaacctaa aatctcctat
     1381 ttagatagca tattatcttc tacagattca gtaacaatta gtcagattgc agcagattat
     1441 gggatgtctc cacaacagat gaataaatta cttcataaac taggtattca gaaaaaagtc
     1501 ggtaaccaat ggttattgtg caaaaaacac atgaaccaag gatacacaaa atctcataca
     1561 actgagatcc cgaaagccga tggtggcact aaaattgtaa tgaataccaa atggacacag
     1621 aaagggcgtc tatttatcta cgaattacta aaaaaagaag gatattaccc tcaaatggat
     1681 ttagaggaaa ttggttagaa aggagtttta gtatgactga cattgcagaa atcactcaac
     1741 gagatagaga aaaaatcaaa gaatatgtcg aaagttcaaa gttcttaact tacaccatgc
     1801 ttgctgaaag atttggaatt agcaaaagct acttatcttt aattttaaac ggtaaaaaga
     1861 cttctgcaga agcaaacaga attatagatt cgattatcac tatgtacgaa ttgtaaaggg
     1921 aggaacagct aatgcaatat ctagaagcaa aaatcccaat tccagaaggc tatgtaatta
     1981 tctcccaagt ggattatgag gagttaaaaa aagctgatga tactggtaga tggatgacgt
     2041 tgccagaagt gctagaacgg attaacagaa aatatgattg gtttacttct agagttttaa
     2101 agaacccaag atatagaaat attatcgata tcgagaaaaa caaaaatgga tttgtctatt
     2161 atccagttga aggaagagac acatatctat ttttaagaag taaaacactt gaatttttag
     2221 aaacaaattt ttcggaaatc ttaaggaggt aagcggatgg gtaaatttaa tagagcacta
     2281 gtattcagcg caccactaat tatctacgct ttaggtcttt ggggaagcag acaagcatta
     2341 atcggcacaa ttgtatatat ggtctggatc tttatggggc ttgatgaagc tgaatataga
     2401 gcgaaaaagc cagtcgggag ggactgactt aacctaatta attaaaaatt gtaacaaata
     2461 acgcaaaaat aaagctaatg aaattgtatt gttaggatct actccaatag ttatatttac
     2521 aatttcggtt actactagtc ctatcaaagc tgccccagtt tgctctaaat accacatttt
     2581 atctttgtag aaagcaggtg aagcttgttg tttttcagat ggttcattag ttatatcttt
     2641 gttcttggct gtatttattt cttcaaaaag ctttatagaa tcatcaagga ctttcctttg
     2701 ctcattaagg atatcaaaaa acttagcctc aaactcatca gatggattag aagtgtcttt
     2761 aaaaagtaga ttttttgtac taacttccgg ttcggaagaa aatgcttttt ctaattcttg
     2821 ggtagctacc cctattctga agtcgtactt agacatgtca acggcaaatt tttctgtgat
     2881 tttagagata tcagaaaaat aaggttttgg catagtagct gccatttttt tagcaatatt
     2941 aaaacttgga gattctaatt gtttctgtat tgcgaacaaa gaaggaggaa tcatattagc
     3001 aatagatttg ttacgtttct cgacaatttg aatgagagaa tcattgagta ttgcttgcga
     3061 agctataaaa gtatcttgaa ttgcaattaa ttttttagct tgttctatcg caggctgcat
     3121 agcgttaacg acatctagag aaggagcaat ggtttcaccg attaattttg aagaagctat
     3181 caatttttga taataaggag tatctgaaat atccaattat atacaccacc tttttaccta
     3241 aattatacca aaagggagag aaaaaaatgc aataattagt aattttgaaa aataaagaag
     3301 ctgtgactac gagcttacaa gtcgcagaaa cattccgaaa ggaacataga gcagttttaa
     3361 aggcgattga cgatctaaaa gatgggcttg cacaaaagta tgcagaccta ttttgggaag
     3421 atgtgtacat ccatccgcaa aacaaacagg cttatcgaat tgtctatatg aatcgtgatg
     3481 gctttactct tgctgtatta ggattcaaca atactcaaaa agttttagag ttcaaattga
     3541 aatacattga agcgttcaac gaaatggaaa aggcaattcg caaaaataca gcttttgaca
     3601 caattgaaga catgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt
     3661 ccatgcttaa aaatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag
     3721 gaaatacgaa agttatggaa gttctagggg gaaaagaaag ccgagcttat gaagaaatca
     3781 gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat
     3841 atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttac
     3901 cagaaactgc gattcgcatg gaaatcgatc aactgaacag acaacaaaga ctttttggtg
     3961 atgaaaatga atagagctga agcgctaaga atcgggacgg taattgctaa tcgctggtgg
     4021 agacacaata aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa
     4081 caaataaaaa agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga
     4141 gattttagca tatggaaaaa gaactttcca ctctagatca atatttgact gatcctagtt
     4201 ggggcaaatc gaatatcaag gaaacaaata atcgaaaaat cagacgaaat cttttgacgg
     4261 atgaagaact agcatgtgat caagacgatt tgggaaattt tgtgagtatt tgggatcatg
     4321 tctatcttat ccatttatcg aggaagtcca aaaaacctga atatatctat gtcatcgaag
     4381 atggcttgat tgatgcgcta gaagagtatg acagagataa cttgattgat atctcttatt
     4441 acggatcagg taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaaacaaa
     4501 gggacaacga cttttgaaaa actttttagt cgcaagttaa ataaaatact caagaaaaaa
     4561 ggaaattttg attatttatc ttgggctcac gcatgggaga ttatgaaaaa gaatgatcca
     4621 caggcaacgg taactattaa tgagtataaa cactacagag ttgtttctgg aactcatcaa
     4681 gactttcttg ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct
     4741 gtctcagtaa cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga
     4801 aaccaaccag ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt
     4861 gtgaaagcat tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca
     4921 acaccaccta gaatcgatac gaagaaatta aacatgctag agacgattct agaagctttc
     4981 aatgagcaga tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca
     5041 gataaattag ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa
     5101 tgtgccttga tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat
     5161 gagtgtttaa accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag
     5221 acataggtgc aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg
     5281 gctacgatgg tgatcgagtt attgaaattc gttttattga tcctagacgt ttcacagtac
     5341 agcaacgaaa cttcatctat gcactcatag gcgatatttt catcgataca ggcatgccaa
     5401 cggacttctg gaaggaattc ttttacttcc gctttgaagg tgtcacaggg cgcaaaataa
     5461 gcctcaaaga tgaatcgaat acgactgtga gtgatgctaa cgtcttagca aatatcatct
     5521 tagatttcat ctttgaacat catattcctt tcaaagaagg ctatgagatt ttaccagcga
     5581 atcaagaata ttacttctac aaatgcatca caaaaagagt ctgttgcatc tgtggcaaaa
     5641 caggagctga tatcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg
     5701 atcattcaga gtacacattt gcagcactct gcagaatcca tcacacggag aaacacaaga
     5761 taggtgtgat caatttcaaa aataaatatc aaatcaaagg gatcaagtta aaccaggaaa
     5821 caattaagaa acttagaata ggaggataaa aaatattggc tgacaaca
//
LOCUS       DACDVT010000113         5862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDVT010000113 DACDVT010000000
VERSION     DACDVT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.115"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWL52_002485"
     CDS             complement(99..1229)
                     /locus_tag="IWL52_002485"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9779000.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWL52_002486"
     CDS             complement(1878..3080)
                     /locus_tag="IWL52_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9779001.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWL52_002487"
     CDS             complement(3140..3343)
                     /locus_tag="IWL52_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9779002.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWL52_002488"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWL52_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWL52_002489"
     CDS             4880..5692
                     /locus_tag="IWL52_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9779003.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1209 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa tt
//
LOCUS       DACDVT010000114         5630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDVT010000114 DACDVT010000000
VERSION     DACDVT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.116"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(258..624)
                     /gene="ssrA"
                     /locus_tag="IWL52_002490"
     tmRNA           complement(258..624)
                     /gene="ssrA"
                     /locus_tag="IWL52_002490"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(795..1568)
                     /locus_tag="IWL52_002491"
     CDS             complement(795..1568)
                     /locus_tag="IWL52_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9779004.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGTGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1712..2062
                     /locus_tag="IWL52_002492"
     CDS             1712..2062
                     /locus_tag="IWL52_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9779005.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2043..2759
                     /locus_tag="IWL52_002493"
     CDS             2043..2759
                     /locus_tag="IWL52_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9779006.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2759..4207
                     /locus_tag="IWL52_002494"
     CDS             2759..4207
                     /locus_tag="IWL52_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9779007.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4277..4786)
                     /locus_tag="IWL52_002495"
     CDS             complement(4277..4786)
                     /locus_tag="IWL52_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779008.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4776..5501)
                     /locus_tag="IWL52_002496"
     CDS             complement(4776..5501)
                     /locus_tag="IWL52_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779009.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1922 a          974 c         1108 g         1626 t
ORIGIN      
        1 aatttaaacc ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta
       61 tacgaactaa tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat
      121 atattaaaaa gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa
      181 aatctgcgaa aataaaaaat gctcaaaccc ttataaaata aggatttaag catctagcga
      241 aatctgctga aatctcacaa tggagacggc gggagtcgaa cccgcgtcca aacacatcgc
      301 cacttaaaaa tctacgttca tagtcaactc atttaaggat tcactttaca gcttgccgag
      361 tgacaggcat tctgtatcgc tagtctgatg ttctcttttt caacttacag acggaaagtt
      421 gaaacgtatc ccactaaagt taggaccctt actcgagcac atgggcgatg ccgagaggat
      481 ctacgctaac tgtttttagg cagctaaagc gtaagagttg ttttcgtttt tagcagttat
      541 atttaactgt aacgttttta cgtagacgta acctacgaaa cgcaattcaa gctcgactgt
      601 gcctgtcgaa tccgtaacgt ccccgttagc taacaagggt tacgtgtatt tttcatacaa
      661 aattagtata gcatatattg ggataaattc aataaaaaag cgaatcatat cccattattt
      721 atataataac tgcccctttc ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc
      781 ttcaaaaaaa gatcttaaat cagatcaaaa tacttcaatg cgtggacaat tccgccttct
      841 gtatttttct ttgtgataaa ggtagaaagt tcttttaatc cgtcataggc gttgcccata
      901 gcgattttgt tgtcgcaagc ttccaacaag gcaaaatcat tcggaccatc gccaaatcca
      961 aaagtaggaa ctttttccag ctgaagttct ttttgcagac gtttgacacc tgtccctttt
     1021 gagattcctt tcttcactgt atcgatcgag taaggaccat tccgataaaa agttagctca
     1081 ggaaaatgtt cgtgatagta ttcgtcacct gattgagata agatcagcaa catatttact
     1141 gtgttttctt taaagcctaa aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa
     1201 gcattgatca ttgtttggct atgaccggta caccagattt tctgatcgtt atagtaagaa
     1261 agttcatgac cgttcaactg tacatgatca tacatttttt gacattcttc tgaagaaaat
     1321 gtttgagaat agatttcttc gccgtctact cgaatgaaag aaccattcat ggtgaccgca
     1381 gaagtaatgc ccgcatcttc catgatagag tggatttctg cttctgtacg tcctgtagcg
     1441 atgacgggga gaacattgtt ttcttttaat gcgttcatag cttttgccac ttcgggtgtg
     1501 atttttgatt tcgcatctag taatgttcca tcaagatcaa agaaagtgat tgcacgataa
     1561 tttgacacaa ataaaactcc ttttttattg gaataagttt atcttaacaa agaatgaaca
     1621 aaagacaaac ctttcgtgaa gaattcctga acatattggt tcagtcaaca atttagagta
     1681 tactataaga agagaattta ggagggaaag aatgattttt ttcagtattg gtatcttatt
     1741 cttaataatt ggcagtcttt ttcggattct gccctcaaaa ggaaatctgc ctttttatgg
     1801 gtatcattcg cctcttgccg caaaaacaga cgctcattgg cggctggctc aaaagacaag
     1861 cggaaactgg ttttttctta tgggcttact gatggcattg atcggctatt atttaaagac
     1921 atctgggcat acgaattatt ttctgattga aatgcttctg ctagtatttc caattatgcc
     1981 tatttttatt atgacagaaa agaaattaca gaaatatgac ctggaaactg gagggaacga
     2041 taatgaatat tttaatgatt gaagataatc aatctgtttc tgaaatgatg cagatgtttt
     2101 tcttaaatga aggttgggaa gcaactttca agtatgatgg aaaagaaggg ctcgatgcct
     2161 ttttagaaaa cccacaacat tgggatatga ttacactaga cctgaatttg cccacactgg
     2221 acggtatgct agtaggaaga gagatccgca aagtttcaag tactgtgccg attatcatgc
     2281 tgacagctag agattctgaa agcgatcagg tgatcggctt agagatgggg gcagatgatt
     2341 atgtcacaaa acctttcagt cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc
     2401 gtagtgagtt ggcagaagca agagaagaag cagtagtttc agatgatcat tttgatatcg
     2461 agactagtca tttcaagatg aatacgaaaa cacgagaagc gtatctggac aaccggccaa
     2521 ttgaaggact gacgcctaaa gagttcgatc tattgtttac tttagcaaaa aaaccacgtc
     2581 aagtattctc tcgggagcag ttgcttgaat tagtctggga ttatcagtac tttggtgatg
     2641 aacggacagt ggacgcccat atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac
     2701 aagtcatcca aactgtttgg ggagtcggct acaaattcga tgattctggt gttgcctaat
     2761 gcgttatttg tatcagcaac tcttagcttt ctgcggaatg gtcgcgttga tcttattgat
     2821 cgttggagtt tcttttacac aactgacaaa acaaacgatt gaagaaaaca attaccagca
     2881 actgtttgga tatgctgaat cagttgaaaa gacaacgcaa acctatgccg atacgctccc
     2941 gcagtttagt caagatcagg cgtttcaaaa tgcattgctg ctgacagagc aagtactgac
     3001 agaacaaaat gtgaacttta tatttatcga taaaaacgaa cgtgtcattt atccgactaa
     3061 tacagcttta ctgaatttta ccttaacagc tgatcaatgg aaaaatctga ggaatggacg
     3121 tcaagaaaaa ttcacatcga ataaaaatat tttaggacaa gcacaggcga cctcttacgc
     3181 acttgtgcca tttaatttga atcatgaatt ctatggagca cttgtagtta gtcaacctgc
     3241 gcgaaatatc gataacagcg ttcgtccaat tacactgaac ctgttcaaag gatttatttt
     3301 ttccagtatc gttgcagtta ttgccagcta tgcttttgca gcattccaag ttaagcgtat
     3361 caatcggtta aggaatgcga cgaaagaagt gacgaatggt aattttgatg tacagctccc
     3421 cgtacatgac aaagatgaat ttgatgagtt agcagatgat ttcaataaaa tgacgaattc
     3481 attgaaagaa tctcaagcag aaattgaaga acaagaaaat cgtcgaagac aatttatggc
     3541 agatgcttct cacgagatga gaacacctct tacgacaatc aacggacttc tggaagggtt
     3601 agaatacaat gcaatccctg aaaatcagcg agaaaacgcg attaaattga tgaaaaatga
     3661 aacagagcgt ctgatccgtt tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa
     3721 tcaaatcagt atggtcatta aaaaattcaa tggaacagaa acattgaaaa atattgttgc
     3781 acagttagaa gctaaagcag aagcagcagg agatacattg acattgaaag ccgttgatga
     3841 tattgatgtt tacgctgatt atgaccgatt tgtccaaatc atggtcaata ttattcaaaa
     3901 cgcgatacag tttactgaaa acggccaaat cacgattact ttagaaaaag gctatcttga
     3961 aacgatcatc acgattgaag atacgggaat tggaatgtct gaacaacaaa tgaaaagtat
     4021 ctgggatcgt tattataaag tagatccttc aagaaaaaat acaaaatatg gtgaatcagg
     4081 attaggttta ccaatcgtgc agcaacttgt acgcttgcat aaaggaaaat tagaagtaga
     4141 aagcgaacta ggaaaaggaa caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa
     4201 agagtaaaaa acaagcggta taaaagatca tgaatcgatc ttctataccg cttgttttta
     4261 ctataaaatg attgatttag cccgcgggct ctaaacgatc aatataagga ctttgagcaa
     4321 tagtagcatc atgagtaaag agtaagatac cttgattttc ttcatgagct aaataagaaa
     4381 gcaagtggag tagctcttgg atctccaata cactaagttc atcaaatgta tcttcaataa
     4441 caatattgtt tcgcttggaa agcaaggcat gaagtaattg cagctttact ttttcaaaag
     4501 acgtcaactc agctgccgca caattaagca aggaaggaga gatatcaagt ttttcttgat
     4561 aggccaaacg atcttgtttg atttcttttt ccggtacatc caacaggaca tggtctttaa
     4621 gattcagata aggaaacatc ggccaatttt tttggatgat cgcaaaatta tccagttttg
     4681 caacaagaac ctcttttttc aagtgctcca atattttatg ctgtttttcg agaggacaca
     4741 aaataacggt caagtgttca gcatgaatca aatattcagg tttcattacg tccaggcctc
     4801 ctactttgca aatgatggat aaaaaaagtg aaagggaata gactaagaat gagacagcat
     4861 ccactgagcc caataagagt ttgggcaaat cccttaagta tatctgtgaa aaaaagatca
     4921 ttttggaaat cgatggtaaa aaaagtctgt tgatccatcg gaattgtaag tccgcttacc
     4981 gaagtcaaga tatcacggat atctggcagt tcagtagtct ttaagacaag aaaattgatc
     5041 ctttgaagaa gcgcttcata aactgactgg aagcttacga tccacaataa aaccaagata
     5101 caacaaagaa agagaggaaa gaacagctgc cagaaaataa aaaggaataa tttggtttta
     5161 gaaagaccag tcaggtgcca attgatcaat tcctgcttgt tttgcctgac tgaatagtaa
     5221 aagaaaaaga taaaaaggaa ggcaaataga aaaaataaac tgaatatgcc ttttatataa
     5281 aatcggctag ctaattctaa agccggaaga agctctgtag atgcagaagc gccaataaat
     5341 gaaagccgat cttggacttg attggctaaa gccgattgca aatggtaaag agtcaggcat
     5401 gctatcaata aaaaagcaga aaagcttgaa aataaacaat agagtattgt ctgctttttg
     5461 taataaatta atcgagcagt agcatagcgg attattttca tacttttcac ctctataaaa
     5521 agtatagaaa atgaatatga ataaagtatg aaattccttt agtgaaatca cgaattaaaa
     5581 ccagaaaata ttttgtaaaa agcaagaggt tgtgaagaag ctgccctatt
//
LOCUS       DACDVT010000115         5602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDVT010000115 DACDVT010000000
VERSION     DACDVT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.117"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            579..2402
                     /locus_tag="IWL52_002497"
     CDS             579..2402
                     /locus_tag="IWL52_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9779010.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            2416..3825
                     /locus_tag="IWL52_002498"
     CDS             2416..3825
                     /locus_tag="IWL52_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9779011.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            3843..4679
                     /locus_tag="IWL52_002499"
     CDS             3843..4679
                     /locus_tag="IWL52_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9779012.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            4744..5526
                     /locus_tag="IWL52_002500"
     CDS             4744..5526
                     /locus_tag="IWL52_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779013.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT         1750 a          931 c         1220 g         1701 t
ORIGIN      
        1 tttaaacatt tgctagttaa tttttaagaa tactagccca aatcattcta tcgatacctt
       61 tatatctcgg aatgattgaa attggctatt gcttactgat ttttaccaaa aaaacaagta
      121 tcactattgt tgcctatatt gttggaatgc tgtttattcc atcctttact caccaaattc
      181 atcaattttt cccaagagca gaattgttga agttgtgtga tccgttatat gcatttttga
      241 tgctttcacg attttgggaa tttcccgctg gttatgtagg aatggtttta ctggtatagt
      301 taatattaga tatacttatt ttagcagtaa gtatacgcag atatacccta gttgatgtga
      361 tctaactgtt atattaccct atacggtcaa acgaaaagat aaaaaattaa attccctgtt
      421 gattgcgcta tcataatgtg ctattattgg attacagcaa aataacgtta ttggcatgtt
      481 actgattatg caggcaagac caagacaaac taacacttca atgttgattg gggcgtggtt
      541 tgacttggtc ttttttactg ctttaaggag agagaataat ggattacaga gaagtaggaa
      601 gaaatgtatt gcaacaggtt gggggaaaag aaaatgtggt cagcttaacc cattgtgcca
      661 ccagactgcg ttttgaactc aaagataaaa ggaaagccga tacgaaagcc ttagaaaaaa
      721 cacttggtgt aattagtgtg gtggatagcg gtggacaata ccaagtggtg attggcaatg
      781 aagtccaaac agccttcaag gaaattcaaa aggaagtggg aacttcaatt aaaaagggcc
      841 cctcttcaga caatcaagaa agtattattt caagattgat tagtgtcatt tctaccacct
      901 ttacaccgat gattccagct ataacaggtg ccgggatggt aaaagccatt ttggccgtct
      961 tgactttgac tggtgtacta tcagacagta gtcaaactta catcattttg aatactattg
     1021 ctgatgcagc cttttatttt atgccagtac tattagccta tggggcagcc ataaagtttg
     1081 atgtcaaccc aattttggcg attactgttg ctggcatttt gcttcatcca aatatttcac
     1141 agcttttttc tactggcgat gccgtttctt ttctaggaat tccggtatta gctgcagatt
     1201 atgcgggatc agttttaccg attatcttta ctgtttggat catgtcctat gtagagccct
     1261 ttgcggatcg aatttcgcct tcgtttatta aattctttac taagccgatg ctggtatttt
     1321 taattatggg gcctttagcg ttggttgtca ttggaccgtt tggaacgttg ctgaacgatc
     1381 ttatagcaat gggggctgaa tggattaacg gcaaagcagc ttggttaatt ccatttttga
     1441 tgggtggttt gcagccattt ttggtggtta cgggtacagc ttgggcaatg acgccaatcg
     1501 ctactatgca attgagcagt acgggttctg aaatgatcaa tgggccagga atgctggctt
     1561 ctaatattgc tcaatcaggt gctacctttg ctgtagccgc gaaaactaaa aacaaagagt
     1621 tgaagcagct agctgtgtcc actgggatca ctgctttaat gggaattact gagccttcac
     1681 tctatggagt aaccttgaag ctgaaaaaac cattagttgc ttccatgatt ggtggtgcaa
     1741 ttgctggttt gtatgctggc ttgagcggtt tagtcagata tgcctttgtt tcccctggct
     1801 tagcagcctt accagccttt attggagaaa atccaatgaa tattgttcac gcaattatta
     1861 cgtgtgtaat cgcctttgtt gcaaccttta tcattgctta tctaattgga tttgacgata
     1921 cttctgatga atcagagcaa acggcaatag ccgaagcagt cattgttgaa aatccggtaa
     1981 aaggaaccgt gtgtcctatt tctgaagtga acgatgctgt cttttctaag ggactattgg
     2041 gaaagggcgt agccattcat ccagaagaaa acagcattta ttcacctgtt gagggagaag
     2101 tcattactat tttagaatct aaacatgcga ttggtctacg ttctaaagaa ggtattgaat
     2161 tattgatcca tgtggggatc gacaccgtga atttaaaagg ggaacccttt gaagtatttg
     2221 taaaagcagg agatcaggtg tcagcaggac aattattaat caacgccgat ttcgctgcca
     2281 ttagaaacgc tggctacgat gatacagtga ttgtggtagt gaccaacagt gaaaattacg
     2341 caggaattag tgaagcaagc ggaagcttaa aggaacacga accattgatg caaattagtt
     2401 aaggagagga tttttgtgca atacaaacag ctttcagcat ttccagagaa gtttttatgg
     2461 ggtgcctcca ttagtgccta ccaggcagag ggtgcctatc aggaggatgg gaaacaaccg
     2521 tctattattg acaagtatca gcatacagaa ggggtagcta attttgaagt tgcctcggat
     2581 ttttaccatc actataaaga agatatacag ctgtttaaag aattaggctt aaaagctttt
     2641 cgcttttcca tcgcttggac gcgaattatg gatattgaaa ataataaaac caatcaaaag
     2701 ggaatttctt tttatcatca agtaattgat gaatgcctga atcagggaat cgaaccgatt
     2761 gtcaccatgt accatttcga tttaccttat tttctagagg agcagggcgg ttggttaaat
     2821 cgagcaacta tcgattattt tgtggactat gtacaggtat tgcttactga gtatggtgac
     2881 aaggtgaact attggctgac aattaatgag caaaacacga tgatccttca tcctggagcg
     2941 attggtttgc caccaagtgg agagctgcct tcaaaaaaag aactctttca aattaatcac
     3001 catgttttat tagctcaagc tagagtaatt cacttgtatc atcagttgaa tcttaaagga
     3061 aaaatcggtc cagcaatcaa tttaacggct atgtatcaag ctacctccag tccagaagat
     3121 gcaattgcag cccataattg ggaaacattg agaggctggt ctttcttaga tgtagcagtt
     3181 cggggaaaat acaattattt gtttgaaaac tatttgaatg atcgtggatt atatcctaaa
     3241 attgaggcag aggatcaggc aattttatcc tccggtaaac ccgattttat tgcgattaat
     3301 tattattcaa ctgccaccat cgctgccagc aaaaatgacg gatcagatgt ttccgctaga
     3361 gcgggggatc aacagatcat gttgggagaa gaaggtgtct accgggcagc tgaaaatcct
     3421 tatgtagata aaacgccata tggctgggta gttgacccta ctggactacg attaacattg
     3481 aggaaattgt acgatcgcta tgatttacct attttaatta ctgaaaatgg ctatggggca
     3541 cccgacatag ttgaggaaga tagacgaatt aatgaccagg atcgaattga ttatctagcc
     3601 aaacatattc tggctattca agcagcttta acagatggtg tagatgtttt tggttattta
     3661 ccttggtcag cgattgatgt tgttagtacc catcaaggct tcaataagcg ttatggtttt
     3721 atatatgttg accgaaccga tgagaatctt aaagaattaa aacgaataaa aaaagaaagc
     3781 ttcttttggt atcaaaaagt aatcgcaaga aatggcttag cttagagaga acggtgaatc
     3841 taatgagagt aattaaaacg ttaaacaaca gtttaattct ttgcttagat aagcaaggca
     3901 aagaagtaat tttgatgggg aaagggattg gatttcaact aaagaaaggc gatttggttc
     3961 cggctgacaa aatccaaaaa gtatttaaat tagattcact ttctgatgct ctaaaaaagg
     4021 attacattaa actgtttgag gctgctccgg atgatctaat tatttgcgtc aagcgaatta
     4081 tcgattatgc caacgccaag tttgacttta agctgaataa aagtctattt tttaccttgg
     4141 ttgatcactt gaagcatgcc attgaacgca gtcaaaaagg cattgttttt caaaatcgcc
     4201 tgctgattga aattcaaaag tattacccaa aagaatttga gatcggctgc tatgctatcc
     4261 aattgatcaa caaaaaaatg aatgttcagt tgccagaaga ggaggctgga aacattgcgt
     4321 ttcacattgt aaatgcccaa agtgaacaag caaatatgga agaaaccatg ctctcggtaa
     4381 gagcactaaa ggatatcttg gctatgctag gatatctgtt tcccgaaaaa aaggttgata
     4441 aagattcgat gctttactta agatttgtga cgcatttaca gttttttatt gcccgaatga
     4501 ttaagaagga agagttgccg gagaaggatg attttttgct ggcttcagtg aagcagcgtt
     4561 ttccgaaaga gtatcgggcg gccttgaaaa tcaaggagta cgttcaagaa gagatgggtg
     4621 cctcagcaaa tcaggacgaa ctgctttatc taacactgca tatttcaagg gtgtactgaa
     4681 tgaagaaaat atcggctgca caatatataa agacttgttg gttgtgctaa ggctcctgat
     4741 tccttggaaa attcttctaa cagtgcggag gtgaatgttt cgacctctac cagtgaagtg
     4801 gtaaagtgtt cacaagaaac tactagcttg ccagaagagc tacttggaga ttggcagatt
     4861 aataataagg atgctggtat agagatatca gacagtaaag ctgtattccg tataggaagg
     4921 tctcaagtag agtcaaagga ttattatgta gatgtaaaca acaaaatgct tttacatgtc
     4981 tatttttctt ttgagtatga tagtgtgaaa tacattgaag tctctattga aagaactggt
     5041 tatagtgaat ttattagtaa aagcgtggga attttaacta tgcacactaa agatggacaa
     5101 tatataagtt tgggtgtgaa gagagtttcc ggtgagaata atgagacaga agcagttacg
     5161 gagcagacga tggatgaaag aatcgattca tctaagagta atgagacaga agtagatact
     5221 gggaagacga tagatgaaag aattgattca ttcatcgaaa catacacagc tataccacct
     5281 acttggaagc ttgtaaatga agataaagga atcacgataa atactgatac agagatttct
     5341 acagatgctc aatctttatc agatttatta gctaatctaa caggtatcag taatgcagtg
     5401 acagagcaag ttggtaaaga cattccactt cggttacttg aaaatgggaa aaaagattat
     5461 tctgtggtat ttaagaacgg acaggtaact gattacaacg aagtatttaa aaaagttatt
     5521 gaataatgat tcttccatct ttttcattta tttaccctac cccgttgtta tatgaccgta
     5581 cttatacatc tccagtagct ta
//
LOCUS       DACDVT010000116         5528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDVT010000116 DACDVT010000000
VERSION     DACDVT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.118"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(416..1123)
                     /locus_tag="IWL52_002501"
     CDS             complement(416..1123)
                     /locus_tag="IWL52_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779014.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1116..1553)
                     /locus_tag="IWL52_002502"
     CDS             complement(1116..1553)
                     /locus_tag="IWL52_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779015.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1568..1819)
                     /locus_tag="IWL52_002503"
     CDS             complement(1568..1819)
                     /locus_tag="IWL52_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779016.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(1819..2025)
                     /locus_tag="IWL52_002504"
     CDS             complement(1819..2025)
                     /locus_tag="IWL52_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9779017.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            complement(2465..2725)
                     /locus_tag="IWL52_002505"
     CDS             complement(2465..2725)
                     /locus_tag="IWL52_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779018.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRSMGI"
     gene            2800..3003
                     /locus_tag="IWL52_002506"
     CDS             2800..3003
                     /locus_tag="IWL52_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9779019.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3144..3629)
                     /gene="ssb"
                     /locus_tag="IWL52_002507"
     CDS             complement(3144..3629)
                     /gene="ssb"
                     /locus_tag="IWL52_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9779020.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3632..4531)
                     /locus_tag="IWL52_002508"
     CDS             complement(3632..4531)
                     /locus_tag="IWL52_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9779021.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFADTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4544..5137)
                     /locus_tag="IWL52_002509"
     CDS             complement(4544..5137)
                     /locus_tag="IWL52_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9779022.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5377..5511)
                     /locus_tag="IWL52_002510"
     CDS             complement(5377..5511)
                     /locus_tag="IWL52_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAP9779023.1"
                     /translation="MKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK
                     "
BASE COUNT         1470 a         1108 c          917 g         2033 t
ORIGIN      
        1 acgggtaacc gtttatttcc tattgcaatt ggcattagta ctctctcctt tcatcgaaat
       61 aaccctgtga aaaatatggc tgcaatcaac ttgaatatta gactcacaag ggttaacaca
      121 aagcgccaaa agatcaaaca aaataccagt attcctaaga atactacgaa gtttaaatgt
      181 agagctgcac catgacgcat aagtatctcg ccaacaaaca gtacgaacaa tagaggaatg
      241 attttattca tatggatctc cttctttctc ttaggttatc gggctttgcc cgcttatttc
      301 gcaccaccta agcggcgggg gcggttggag cgattggtgc gaaataagaa ccagtccctt
      361 aatttcaagg ttttttgttc ttatttcaca catcctcact tttcggtata aatggttatt
      421 gacctttttt cctttcaaat cgttgatatg gcattcgttt acggtcaagt tcattttgga
      481 tttcttctgt atcgtcgtaa gtgtaggtgt ctaaaaaatc agttaaagtc gtgtaccagt
      541 cgtcgcctgg gttatctact tcttctacat attccctcaa aaatgcttcg ggtacagcga
      601 gttcataatc catccccatt aacacgatag attccgtccc acacgtcaaa aatctagtaa
      661 atacaaggta ataatcgtct accgtcgcat ctttgctgtt tagctcttcc atttcttctt
      721 ccattaattc catgctctca aaaattgaat aatattctga ttctgagtaa tagtgtttcc
      781 tgcaaatcgt gtagctgaca aagttttcga ccagctcact cctttctagg taaacattca
      841 tgatatattt ttctgtttct agtcgtagaa gctccgctcc tttttttaat gtgtcaggac
      901 gacgtttata cacgtctaaa cgattatggc tgactgtaac cttcgtttga ctcgtttcac
      961 tttccatata caactgttct tccactcttt gaataaagta cttcatttct tccatacaac
     1021 taaagtcttt ttgaatccgt ctgattactt cattcaagtg ttctttgttt gttaaaaatt
     1081 gcgaaacatc ttctgttgtt agctgataaa ccaccttatt cattatcttt ctcctccgat
     1141 tcaatcgtat aaaatgcacc atttttttcg atattttcaa aagtatctag ccagtcgcta
     1201 gccattgctt tagctttctt aatctgtgcg aattttgcat aagtcagttc taaggcttga
     1261 tattcggaag gtttgatagt gcttggtttg acacctactt ccaagcatag taaggcttgg
     1321 aaaaaccatt ttccattttg ttttttcagt tcttccagtg ttcgatagta cgtcgcaatg
     1381 agattcattt cgacacctga ttcaatcatt tcttccactg cgactgacaa tttttccttg
     1441 taatgttttt tgggtacacg tgtttcatgg tataggaacg tcatatcctc cattttttgt
     1501 gccattttat ccaaaggtaa tttgacaaat tcgtcatagt aattttgagc catatttttt
     1561 ctccttttta gttctctaat ttggttaacc aatattcgat ttcataagtt tccatgtttt
     1621 cttgataatg cagttcatat cctgcaatct tcactaagtc ttcaaatagc atagtcggtg
     1681 catcctctac taaaggggtt tctatcgtcc agtaccgcgg aataagtaaa gcttctgcta
     1741 ctttttcaga aatgacttct ttgtccacca aaatttgaac gacttctttt acagtcaata
     1801 atttgtgttg tgttttcatc agcgtttctt tcctttctca tcaatcaaaa tataatcgcc
     1861 ataagacaag gtatattttt tcccgcatgt tggacattga tacaaacgat agcttttgat
     1921 acatttcgct ctttctccac aatcgcattc gacagtatgt ggtgcatgat agtttgggaa
     1981 taattcgctg aagggaacac cccaataatc aaatttcttt cgcatgattg attctgactc
     2041 ctcttaggaa agggtttact tcctttcgtt agttctttct ttattgattt gttggtcaga
     2101 atgaatcaac gaaaagcgac aaggacaagg gcgcgcagcg tgccatttac ccttgtcctt
     2161 gtcgcttttt ggtgataaaa ggcaacttgt tgccggtctg acaaatcaat aaagagataa
     2221 cgaacaggaa cgaacctttt ggtgagagga agttttttct attttgtctt gctttgcaag
     2281 acaccatatc ttagtgaacg tgcagcgttc acttcctttc ttctcgaacg gcggtggcgg
     2341 ttggagtgag agaaaggttt ttcaattgag aaggcacttg tgtcttccta aaaaggtctt
     2401 ttcctgtttc tatgtaaaac aaaaagagct gcagtcctac attaggattg caactcttct
     2461 ttttttatat gcccatcgat cgtgtaaact gatcgggatt tctaacatac atgctagcaa
     2521 taactagctg atttcgttta ttgaaatgac ctgtgacggc aatattgtat tttccatcat
     2581 ccataattaa caatttatcg ataatttcaa tatctgcaac tacacaatta atcgattcat
     2641 gaattgtttg taaagtaaag cgaaccaata atggttgtgt ttttattact cggattttat
     2701 caagcaaacc aacatatttg ttcatcacga tcacctcgct aaaattatag caaaaacagc
     2761 taaaattgag aatttttatt cgattttaga tatgctataa tgatcaagac agtaaagttt
     2821 gtgacggtgg ctatccgatt gaaagtgagg tggtgcgtat gtgcgtactt ccaaaatctt
     2881 acagaaagga gaagcctttt gcctgttgaa acggcgctag gattaatgat tagtttcggt
     2941 tctttgatcg caacaatcat ttttggaatc ttagcagttg taaacaacaa tcaaaaaaaa
     3001 taaccgtcag ctttagccgg tcgacgatta tttttcaaaa ataatattaa gctggtcacc
     3061 gtcttaaacg gtgctgttag gggggttgtt agcgcaactc ctcttttcgt atccattata
     3121 acaactttat tgatctttta caatcagaat ggtaaatcat catcactaat atcgattgat
     3181 tgtccgccaa atggattttg tgaagcgttc aatccattgt tgttaggcgt agctacatcc
     3241 cgttgttcaa aggtggtagg gcttggactg ctggacgatt gatcagcgtg cgcacggctt
     3301 tctgtcgctg ctttgctttc taatagttgg aaattatcgg ccacaacttc tgtcacatac
     3361 acacgttgtc cttgctggtt gtcataagtc cgcgtttgaa tccgcccaac tacaccaatc
     3421 aagctacctt ttttagctaa tgtcgccatt gtttctgctg gtttgcgcca aatcacacag
     3481 ttaataaaat cagcttctcg attgccgttt tgattcgtga aattacgatt aacggctaag
     3541 gtaaaagtgg ccactgctgt gccacttgcg gtataacgta aatctggatc tttggttaac
     3601 cgtcctacta aagttacatt attaatcatc tttatctctc catttcttga ttcatttgtt
     3661 gtgatctttt aaactgcctt tttctttctt gattttcaat tttgatcaca cttttcatta
     3721 ggtgatcaat ttcgttgact gtatctgcaa atgaaatgga attcgcaaac gtgttaactg
     3781 agcgatattt ttcccactgc ttttccaaat agtcgtttcc cctgatctta tccatttctc
     3841 tttcataata aaaggataaa ggctttgtat ctccacgttt tgtaaatgta tttcgaacac
     3901 ttttgtataa ctctttcgta tcaatatttt ctagtttcgg aatggtaaat aagtcgtagt
     3961 aatcttttgc tctcgaatta tctgtgccaa agctgaacgt agcacttagc ttttctgcaa
     4021 taatttgtgc tgttggataa gctggaatct ccactttttt atcttcaaac attaattggt
     4081 gatagtgtgt atctacttca ggataaatag aatctcctgt tgtcacatcc attttaaacg
     4141 gcacacgtac tttctctaaa ttagccacca atttaatttg aaatccagga taaaaatcgg
     4201 cttctcttgt ttccttcatt ccttgaatag aaaaaacaat gccttctttc gtgggtttcc
     4261 tagttaaatc attaaaaata ctcttcaatt tttcttcagt taatttgcta tttctaaatg
     4321 tcgtatcgat atcaattgtt gtccttctat cgattccata ttttgatcct aataaaaatc
     4381 cacctttcaa aatgaagtca tttttgtact ttgattcaga aatttgttca attaaacgat
     4441 ccatcataaa atgccgttgc actaactgtg catttaatcc tgtactcttt gctaaatttc
     4501 ttgtttttgc tgcaaattgt gtcggtgtca tctttttcct ccatcaatat aagctttgca
     4561 tgtggtattt catttctttt tctacatgta gttttgtcat gtaatctctt aatttacttg
     4621 gatctctcaa tgggtcttcc atgtactctt ttaatgcttc taatttcatt tcatattcaa
     4681 tttcatatct cggattccaa atatcacaaa gtgtacgctc tttatcatac atagtaatag
     4741 gatttccttg aaaggaagtt ccctctactt ttcctagttc ataaacttct ttcttcacga
     4801 aatgaaattg taactgctgc tcttttgtaa ttgaacgatc aacgttatag ccatggggaa
     4861 aagttaagtg aatgaatttc ggcatattcg tagaaagctg gtaaatatct aaagtcgttt
     4921 catgagagaa gattcccctg gaaaatcgtt gctgaagcca aaataaatca tctattccat
     4981 gaccatcctc tacataataa ccacgatcaa ctttgatcaa ttttccttga ttcaccaatc
     5041 tttgtacagt ggttgctgcg attcctaatt ttctggcttc cttcatagat aaatttccat
     5101 cgcgaatagc caacaattct ttcagtttct cttgcattta ctcccccctt ttcgttacaa
     5161 atacactaca ttaaatatat catgatagtg tatttgtaac aaggttaatg tctatcgatc
     5221 taattcaatt tctttttgaa tccgctgctg gttctttaat tgctgcagtc ttcgtttgct
     5281 ggcacgcatt ttttcctgtt ctgaacgaat tttggctttt tgattttcga tttcttgttg
     5341 tttgtgtgtt gtattacgca taagattaac cctccattat tttgtagtgc gtcgtgttct
     5401 tttcttgaag ttcgtttttt tcttttcaga aaattcaaag cctgtagtga agtcttcttt
     5461 taaaacgatc gttgcttcaa acgaaccctt ctgacctttt aaccctttca ctggttggct
     5521 tgttcggc
//
LOCUS       DACDVT010000117         5508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDVT010000117 DACDVT010000000
VERSION     DACDVT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.119"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            106..435
                     /locus_tag="IWL52_002511"
     CDS             106..435
                     /locus_tag="IWL52_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9779024.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(722..1207)
                     /gene="vanZ"
                     /locus_tag="IWL52_002512"
     CDS             complement(722..1207)
                     /gene="vanZ"
                     /locus_tag="IWL52_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP9779025.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1360..2271)
                     /gene="vanY"
                     /locus_tag="IWL52_002513"
     CDS             complement(1360..2271)
                     /gene="vanY"
                     /locus_tag="IWL52_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9779026.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(2699..3307)
                     /locus_tag="IWL52_002514"
     CDS             complement(2699..3307)
                     /locus_tag="IWL52_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9779027.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(3313..4344)
                     /gene="vanA"
                     /locus_tag="IWL52_002515"
     CDS             complement(3313..4344)
                     /gene="vanA"
                     /locus_tag="IWL52_002515"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9779028.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(4337..5305)
                     /locus_tag="IWL52_002516"
     CDS             complement(4337..5305)
                     /locus_tag="IWL52_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9779029.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         1618 a         1186 c          952 g         1752 t
ORIGIN      
        1 atgaaattgc ttataatagt gtattggaat taaggtagat tgacaaaaga gtgatcaaat
       61 aggtatactt cattcaaata atcgtttgaa tgtgaggtga gatgaatgaa aaaagtttgc
      121 caagtgactg tcgtacataa agaaaaagta aatggaataa aaaataaatt ggatcaaaaa
      181 gatttttcaa acttacttgt gttagggaaa tgtttcagcg atttttctag aattaaaatt
      241 ttctatgctt tagaaaccta taaggaaatg tgcgtctgtg atttagcaga aatacttact
      301 gctagtgtcg ctacaacgtc acaccattta cgttttttaa aaaaacatgg aatggcaaaa
      361 tcacgtcaag atggaaaagt agtttattac tctttagcga atgaagacat cctttccgct
      421 attcgtgttt tttaaagtta tcagaaaatc tatctatgaa atcttagttt gggggtaccg
      481 tcaggaaaat gcggatttac aacgttaagg aaatttatta aagagaccaa ttcctcagca
      541 ctaaaattga taattgggct ttctttgtta ctatagtgtt aggtaagctt tttactttat
      601 caccggtttt cccctcactt cacacctacg ggcgagtttc cccacatccc gttttccatc
      661 taatccaatt cattcaatat ctttatgtgt atgaaagatc agattgcttg atttagacat
      721 cttacacgta atttattctt aaatgggtac ggtaaacgag caataatacg agcaaaagta
      781 tacctacaaa aataataact ctgtctaatt ttttttgatt catatgctta ttgcttaaac
      841 catataattt cagtccaaga aagcctccaa cagtatttgt aattacatct gttatgtctg
      901 tcgctccaat agcgaagata aattgaatta tttcaaaagt aagacttaaa accagtacaa
      961 aagcaaactt aggtaaaaat ccgatttctt taaaattgac attcaaaagc aagccaaatg
     1021 gaataaagat tataacatta tctatcatct ctctgaaatt cccagtagca gtaaatggag
     1081 tcaagttaag acttctttga tgataattaa atactgataa aatattgtat tgtaatttga
     1141 ataacactaa ccagattagt gtcactaaat ataaagctag caatcctcta gataatattt
     1201 ttcccaatat aatttcctct aattcttttg tttaagttgt atcattagta tagcaatgtc
     1261 ctagttaacg tgggttttaa attaaaaagc taacgttacc ccaaaagtgc tagtacaata
     1321 atgctgttca ttaatttcgt tggtttcatt ccgccatcct tacctccttg aattagtatg
     1381 tgttgatccg ggaaacactg tcacaattac accgtctata ttgtttcctg atatctcata
     1441 acgaagatta gtcggcacat gaatggtggt atttttagta acaggataat aaaagatctc
     1501 atatttttcc ccatttacac taacagaaat ggttttttct tcttttaggt aatccatata
     1561 ttcctcgaga acgaaattct tttctttcat aatcgcacta tgtggtaaac caacatagcg
     1621 aatatgccat agttcatatt gaattcctgt taactctgtt ttgtcctctg gataacgtaa
     1681 aatgaacccg tatttccaag cattttcttc tatccacttt ccttcagggg ctcgttccat
     1741 tttcgtcaag cttgatccta catctagtga taaacctgaa ttatgctcac tataacctgc
     1801 tggtaaggca tactcagccc ccatttcttg gtaaagcaca ctttgctcat caaagtctcg
     1861 atagccacta ttaataataa aatgactaac gccacccttt acagcatcat tgaccatctc
     1921 tgaaaatttt tgtgctattt cttttgacat ataaatatta ctatcaagca acccgtatcc
     1981 atttattaat tcgtcatgtt tagataaatt cacgatatct gacttcacac tttcttggcg
     2041 aacaggatat ttactattga ttaatagcag atttccttga taaacctgtt cttctgtaat
     2101 tgttttctct tgggtatttt cagaagtccc actattttct aaatgttctt tgggattttg
     2161 atcataattt tgaaattcga ctttttcctg agaaaacagt gcttcattaa cgtagtcata
     2221 acctaagtat attaagaata acaataacaa taaaaaaaac aacttcttca ttttcagtct
     2281 cctccttaac tatatagttg gaagtatgaa atatttctag aatcttcccc ttaaacggca
     2341 caatctcgtt tgagattgtg ccgttttgcc ccgtaaatta cagtcaatta tttcgcccta
     2401 ctggatttac tattatcacc aatgtagatg aattttcata agtgataccc atgctgaatt
     2461 taacgaggct gaactgcctg ttgacaatgg gcggtgtgaa ataccacccc tctatgcgat
     2521 cagtcttatt atcaggctat cttgccgcag cgcacgcaga taatgaccgc aatatcgctc
     2581 tgccttacat cttcaatact atctactata tataaattcc ctaagaacca gttacatacg
     2641 tcgggtttcc tgccgaaaga gttagcttat atagtttgtc cgtgcaacgg ttaaaagttt
     2701 atttaacggg gaaatcaaaa tagctattgg ggtatggttc gtctcttaat acatagtgcc
     2761 accattcgag gctatatgct tcaaacccac tgttttccat gatggagcgc aaacgtctgc
     2821 gattttgcgc ttcattgcat gatattccat ttgccgcatg atgagagcgt tcatccataa
     2881 aatcaaatcg gctccccatt ggtacaagct cacccgtgtc taatcgataa agcgtaagat
     2941 caatggcact gccgcggcta tggcttgatt ttgaagccac gtatcctttt gaaatcatct
     3001 cagttcggtc aatattggga taataacttt cctttgtcag gttattttcc ggctgtgcag
     3061 cccattgcat aaaacagttt acagcacgat taggacggta accgtcccat agaagcaatc
     3121 cgtacccttg ggtagcagcc agttcttttg ccttcaaaag cgattcagcc aactcgtatg
     3181 tccctacaat gcgatttact tcataaccgt caaccggttt tccggtgaaa ttatcccaag
     3241 tggcatattt agcgtcccaa cgaacaccgt gtactatttc atctaaaaaa gtaaatccta
     3301 tttccatgct tatcacccct ttaacgctaa tacgatcaag cggtcaatca gttcgggaag
     3361 tgcaatacct gcagcggcca tcatacgggg ataacgactg tatgacgtga aaccgggcag
     3421 agtattgact tcgttcagta caatgcggcc gttatcttgt aaaaacatat ccacacgggc
     3481 tagacctcta cagccgagcg ctttatatat tttttttgcc gtttcctgta tccgtcctcg
     3541 ctcctctgct gaaaggtctg cgggaacggt tataactgcg ttttcagagc ctttttccgg
     3601 ctcgacttcc tgatgaatac gaaagattcc gtactgcagc ctgatttggt ccacctcgcc
     3661 aacagctaac gcggcactgt ttcccaatac cgcacaaccg acctcacagc ccgaaacagc
     3721 ctgctcaatt aagattttgc tgtcatattg tcttgccgat tcaattgcgt agtccaattc
     3781 gtccgcgcta ttgacttttt tcacaccgaa ggatgagcct gaacgcgccg gcttaacaaa
     3841 aacaggatag gtaaacgtag ctgccaccgg cctatcatct ttattaataa cccaaaaggc
     3901 gggagtagct atcccagcat ttttcgcaac gatgtatgtc aacgatttgt ccatacaaat
     3961 tgctgagctt tgaatatcgc agcctacaaa agggataccg gacaattcaa acagaccttg
     4021 tatggatcca tcttcacctg acttgccatg caaagctgaa aatgctacat caacatggtt
     4081 gatttcatat tcatggttct ttttaacaag taatccgtgc atttttttat ccggcgagag
     4141 tacagctgaa tagcaattgt cgttttccca ttccgcgcaa ggtttttcgc acattttcca
     4201 tacaccagat ttcgtaattc caatgtataa cggctcgtat ttttctttat taatgttagc
     4261 ggctatctct attgcagatt ttaccgatac gtcatgctcc tctgagcaac ccccaaacag
     4321 tattgcaact tttattctat tcatgctcct gtctcctttc aaaatccaaa cagtttttaa
     4381 tggttttttc aacggtatca cgcaacgctt gctcggtata ataggccgta tgcggtgtga
     4441 ttatcacgtt aggcattctt tgaagtttaa gtaaaaattg attatcaatt ggtttttggg
     4501 tgcaatcaga gtagaaaaac tcttcctctc cttccaatac atccaatgcg gcaccgccca
     4561 gtttcccgtt ttctaatgct ttaaccaact cataggtatc tacaagtgga ccgcgcccag
     4621 tattgataag aaatgctcct tgcttcattc tctgtatttg ttcgtggctg ataatatagt
     4681 gcgtatccgt attgagcggc acatgaagcg taacgatatc gctattttgc agcaactcat
     4741 caaacggtac atagtttacc tctatacttc ggctgcgact ataagccaac actttacatc
     4801 caaatcctcg cagccgctca ataaccgctt tgcctatctg gcccgttccc accacaccaa
     4861 ctgtcatgtc gctgagtacc ttgccacggt cgctgtccaa cctgaaatca tgtttttcca
     4921 cagagcgcac aatcgatttt acgttgcgta ctgccataag aattagcatc atagtataat
     4981 cggcaacgct atccggcgag tacgccacat tgtcgacagt gatgcccatt ctcttagcag
     5041 cagttgtatc tatatgattg cagccgatgc ttcgggtaga aatatatttc acaccggctc
     5101 tcttcagcgc aagaagaata gaggcggaaa tctctgattt atgtcccaca ctgatacatt
     5161 gattgaaagg cgcggatttg gcgttggatt ccgacacgtt ggcgttaatt atcgttgcca
     5221 taacgccaaa gcgaggcgaa agagcatgga atgcatctgc ctcatcctgc tcacatccat
     5281 aaacagtaat gccgatgtta ttcataatta agaccaaccc tttctgtgaa aggcaccaaa
     5341 taaaacgggg atgccaatgg taatcttgtc aaatctttct ctgaagaacg aaaacggctc
     5401 gttcttacgg tattaagtct accgtgtaag aacgagccgt ttcttaatat taaattgtta
     5461 atttcctaag aaaaagtaaa gataaccttg agattttcct aaaaaatt
//
LOCUS       DACDVT010000118         5481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDVT010000118 DACDVT010000000
VERSION     DACDVT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.120"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(118..1080)
                     /locus_tag="IWL52_002517"
     CDS             complement(118..1080)
                     /locus_tag="IWL52_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9779030.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1082..2521)
                     /locus_tag="IWL52_002518"
     CDS             complement(1082..2521)
                     /locus_tag="IWL52_002518"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9779031.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2706..4652
                     /locus_tag="IWL52_002519"
     CDS             2706..4652
                     /locus_tag="IWL52_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9779032.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4916..>5481
                     /locus_tag="IWL52_002520"
     CDS             4916..>5481
                     /locus_tag="IWL52_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9779033.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIE"
BASE COUNT         1739 a          940 c          943 g         1859 t
ORIGIN      
        1 gtctaacttt tgggggtcac tacaagttga caactccttt ttgtttacaa atcaattgga
       61 tatctacaaa ttgtattttc taacgaattt tatagacatt aaaaaattaa tttaacgtta
      121 aatacttcct ccagaaagta ttgagattgg taaaatataa tccttatttg tcttttcgcc
      181 tttaattttt ttcagaagaa tatcaacaat aagacttgca atttcatcaa ttggttgtct
      241 tattgttgtc aattgtggga aaaatttctc aataaaagag gtaccatcgt atccaataat
      301 ttttaaatct tctgggatat taagtttaag ttgatgagca attttcatag tgagaatggc
      361 tgttaaatca tcagaaacaa aaataccatc tggtttgtga tttataatag ttgactttat
      421 ttccatttca cgacggatag tagacagatt attaggaatt ttgaaaattt ttccatttgg
      481 attttgaaaa gaaaaaccta gtgcacgtaa ttctgtggga gaatccgtgt tatcatttcc
      541 tgtaatcata atggtatttt gacaaccatt tttttgtaaa gtcttagctg caagttttcc
      601 cccttcaaag ttatctgaag aaacaatgga aacattagga gctaaatttc tatcaaaagc
      661 aacaataggt gctctcacac gctcataatc agcaattcca agattatggc ttgatgagat
      721 tatgccatca acttggttgg cttcaagcat ttcaatgtat tctctctctt tagcaggatc
      781 attttgacta ttgcaaataa tcgttttata tccatgttta aataactcta tttcgagata
      841 ctcaatgagt tcagagtaaa agatattact aatattagga aaaataagac caataagctg
      901 aggtgacttt ccttgaagtc cacgtgctaa attatttggt ttataaccaa gagttttcat
      961 agcttcatga actttttgtt ttgttttttc tgataaatat cccttattat taatgacgcg
     1021 tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca tcagttaatt ttgctaccat
     1081 tttaatattt taattcaaag tattttccag taggttcgcc tgatttgatt tgaataccat
     1141 tttggtgatt ttcagggaat acacgactag taaacacttt ttctccttta ttaataaaaa
     1201 tctcaatgat tgagttatct acaaaaatgt tagcagttgt ttctttagta tcaattgagc
     1261 attcacgaat cgtaccaaag tcagtggcat attgaatgcc tgctttgctg cgatctaaaa
     1321 taatttttcc ttctttggta tctacaatca agctaagtcc atttccttta ctatcagaga
     1381 aaagaagtaa ctccgatttc tgatttggag gaaaagttag ttcaagctca taagtattat
     1441 ttgtttctgc ttttgctgta aattgttcag aactagcacg taatgatgta atagcttcta
     1501 ctggatattg gtaaagttta ccatctttta ttgaaagttc ttttacaaga ctcattgctc
     1561 cctgataatc atacttatca gttggataat cgatatcagg tagtccaatc caactgacaa
     1621 tcagagcacg accatcagga gtattgaatc cttgagtcgc atacgcttca aatccataat
     1681 caagattttg aatttctgaa gcgcccacaa gttttacttt ttctatgtca agcgattgac
     1741 agatcttaaa agtattcgga tagatattcc cataatttag ctcagattta tccaaccctt
     1801 gaggacaata aatgagaaca ggttttttgt ctacaaatac tagatttgga cactcaatca
     1861 tgtattctga accagtgccg ccaaaatcaa ggttgccaac ttcctcccat gtttccacgt
     1921 tattgtcatc cgctttgtaa agtttgataa agcctgattt ttctaaattt tgtgctccaa
     1981 caatcgcata caattgtcct tcataattaa acatttgtgg atcacgaaaa tgatcagtta
     2041 catcagcagg tttatgaata agaacattct cacctttcgt aatgttgtag tctttatcca
     2101 tccatgctcc gatttgaagt gtatcacgtt cccaattttc atttcgaacg tttcctgtat
     2161 agagtaagaa tagattcccc tctatttcat atgcagaacc ggaataagcg ccgtggctat
     2221 ctcgttttgt gtctggaaga agttctgctc ctgttacatg aaaatgaact aaatcatctg
     2281 attcattgtg tacccattct ttcaacccat gagcagctcc aaatggccaa ttttgataaa
     2341 aaagcgtata ttttccgtta aaaaaagaaa aaccattagg atcgtttaac aatcctgttt
     2401 taggttcgat atgatagcta gcacgccaag gtgatcgttt tacattatcc ttaattttat
     2461 ccatctcatc tttagtccaa tcagaataag cacgatatcg tatttctcga ggtaagttca
     2521 tattttactc cttcaattta tctttttaca actagtatgg taaacgtttt ccgttgaaaa
     2581 atcaatcatt tcatttaaaa aaataaacta tatgacaaac gtttgacata tttttaagat
     2641 atgctaaaat aaatttgcat ttcagtgaac cgttttcacg taaaaataaa aaaggagtga
     2701 ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca agcggtcgga aaagataacc
     2761 ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt actaaaagat gacacgaaag
     2821 tgaatcaaaa agctttagac gaaaatccag atgtgaaaag cacatttaaa attgatggac
     2881 aatatcaagt gattatcggt gcaggtgatg ttaatttcgt ttatgacgaa cttatcaaaa
     2941 aaactggact ttcggaaatt tcaacagatg atttaaaaca aattgctagt aaagataaaa
     3001 aattcaaccc cattatggca ttaattaaat tattatcaga tatttttgtt ccaattatac
     3061 ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa ctttttaaca gctgctggac
     3121 tttttggacc aaaatcaatt gaagaaatgt atcctgcgat tgaaggtatt tcctcaatga
     3181 tccaactgat gtccgctgca ccattcatgt tcttaccaat tttagtaggt atttcagctg
     3241 ctaaacgttt tggagcaaac caatttcttg gtgctgcaat tggtatgatc atgacaacac
     3301 cagatcttgg tggctcaact gaatattgga atatttttgg ttatcatgtt tcacaaacaa
     3361 actatgctta tcaagttatt ccagttttag cagctgtttg gatattatct atattagaaa
     3421 aattctttca taaaaaattg ccatcatcta ttgactttac attcacgcca ttattatcag
     3481 taatgattac tggatttttg actttcacag ttattggccc tgtaatgtta ttgttgtcta
     3541 atggaattac agacattatt gtttggctat ataacactac tggatttatt ggaatgggta
     3601 tttttggagg aacatattca cttatagtca tgactggttt acatcaatca tttccagcta
     3661 ttgaaactca attactttca gcgtggacaa atggtatcgg acatggagac tttatctttg
     3721 tagttgcatc tatggcaaac gttgctcaag gtgctgcaac atttgccatt tggttcttaa
     3781 ctaaaaattc taagacaaaa agtcttgctt cctctgcggg tgtctcagca cttcttggaa
     3841 ttacagagcc tgccttgttc ggagttaact taaaataccg ctttccattc ttttgtgctt
     3901 taattggttc aggaattgct acagcagtag caggattact taaagtagtt gctgtttcac
     3961 ttggttcagc aggtttcttg ggatttcttt caattaatgc aacatctatt ccattttacg
     4021 tattgtgtga attgattagc tttgtagttg cttttgcaat tacttacttc tacggtaaaa
     4081 caaaagctgc tggtatcttt aaagcagaag aaattgctga acaaacacca gttaattctt
     4141 caaaagtagc aacaacagat ccaatatcta ctgatgttga atcaacacaa acaaaagttt
     4201 catcagaaac aattgttagt cccatttctg gtaaagtgat tagtctttct tcagtaaatg
     4261 atccagtctt ttcatcagaa gctatgggaa aagggattgc cattaaacca aatggaaaca
     4321 cggtccattc tccagtagat ggtactattc aactcacgtt tgaaactgga catgcttatg
     4381 gtattaaatc aaataatggt gctgaagttc taattcatat tggtattgat actgtatcaa
     4441 tgggaggaaa aggatttaca caaaaagtta ctactaatca aaaagttaga aaaggcgata
     4501 ttcttggagc atttgaccgt tcaatgattg agaaagaagg tcttgatgat acaacaatga
     4561 ttattgtgac caatacttcg gattattcag aagtaaatat agtagctgaa aatacagtca
     4621 ctgagggtac tgatttactt gaaatcaaat aaatatattt ccctgagtaa agatggaatt
     4681 ttctttccaa aatcaaataa tatagattaa tcaaatattt gagaataaca ttttatttaa
     4741 tcttttcata tatcaattgt atgaaaaatt attagaattt taaaaaatga taaaaattca
     4801 aaaagaaaaa atagtagtta tttacttgtc atgttactaa ataaaaaaat aatttaaata
     4861 tgatatatag tcatttattt agtgatttca ttctaaaata aagggagaaa tgtctatgct
     4921 atacggtgca attgaagcag gcggcacaaa atttgtgtgt gccattggcg atgaagaaat
     4981 gacgatcaag gaaagagtga gctttccgac aacgacacct gaggaaacaa tgcctttagt
     5041 cattgacttc ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg
     5101 gcctatcgat atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaatt
     5161 ggcatggcaa aattttgatt tcatcggtac catgaaaaaa gaatttccga tcccgatttc
     5221 ttggacaaca gatgtcaatg cggctgccta tggtgagtat gtcttcggca gtggcaaagg
     5281 attgagcagt gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga
     5341 agggcgcttt atcgaagggt ttagtcatcc agaaatgggg catatgctcg tcgtccctca
     5401 tccaaaagat gactttgctg gcagctgtcc atttcatggc aactgtttag aagggatggc
     5461 cgctggaccc gccatcgaaa a
//
LOCUS       DACDVT010000119         5302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDVT010000119 DACDVT010000000
VERSION     DACDVT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.121"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1553
                     /locus_tag="IWL52_002521"
     CDS             <1..1553
                     /locus_tag="IWL52_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9779034.1"
                     /translation="EVKQEGQQHPVWLHFGGGNLYRGFHAEIAQCLADQNELETGVVV
                     CETFDEEVVEKAYHDYNNDILEIVMSGDGELKKRLLAVTADSLYCNPKNIADYQRVVK
                     YFEDPKLQFTTFTITEKGYSLKDSKGQFFSVVKADIKSGPSNPQHTMSIVTSLLYSRF
                     NAGELPIAMVSTDNFSQNGQRFQDSIFTIAEAWQKEGYVSEKFIAYLKDKEKVTFPWS
                     MIDRITPNPSENVKEDLLMMGLEDLDIIHTKKHTNIAAFTNTEVVHYLVIEDSFPNGR
                     PKLEKAGVIMTDRETVDKADIMKVTTSLNPLHTALAVTGCLLGYQSISAEMQDDDLVG
                     LIKGIGYLEGLPVVENPGIIDPKQFIDEVINKRLPNPYIPDTPQRIATDTSQKVAIRY
                     GETIKKYVEKDGNAENLTFIPIAIAAWLRYLLAVDDQGENFVPSPDPLLVELQDKLSV
                     LKLGEQDPSKIHCAVQEILENKEIFGLDLYQVGVGKKTEKIFCEMLVGPQAVRKTINK
                     YITEVGGK"
     gene            1556..2635
                     /gene="uxuA"
                     /locus_tag="IWL52_002522"
     CDS             1556..2635
                     /gene="uxuA"
                     /locus_tag="IWL52_002522"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9779035.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2709..4136
                     /gene="uxaC"
                     /locus_tag="IWL52_002523"
     CDS             2709..4136
                     /gene="uxaC"
                     /locus_tag="IWL52_002523"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9779036.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4220..4867
                     /gene="eda"
                     /locus_tag="IWL52_002524"
     CDS             4220..4867
                     /gene="eda"
                     /locus_tag="IWL52_002524"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9779037.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            4987..>5302
                     /locus_tag="IWL52_002525"
     CDS             4987..>5302
                     /locus_tag="IWL52_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9779038.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISA"
BASE COUNT         1819 a          723 c         1126 g         1634 t
ORIGIN      
        1 aagaagttaa gcaagaaggc cagcaacatc cagtatggct tcattttggt ggaggaaatc
       61 tttatcgagg atttcatgca gaaattgctc aatgcttagc agatcaaaat gaattagaaa
      121 caggagtagt tgtgtgtgaa acatttgatg aagaagtagt ggaaaaagct tatcatgact
      181 ataacaatga cattttggaa attgttatga gtggagatgg tgaactcaaa aaaagacttt
      241 tggcagtaac ggctgatagt ttatattgca atccgaagaa tatagcggac tatcaaagag
      301 tggttaaata ttttgaagat cctaaattac agtttacaac gtttactatt actgaaaaag
      361 gttacagttt aaaagattct aaagggcaat tcttttcggt agtcaaagct gatatcaaat
      421 ctggacctag caatcctcaa catacgatga gtattgtaac atccttgtta tatagcagat
      481 ttaatgctgg agagttgccg attgcgatgg tttctactga taatttctca cagaatggtc
      541 aacgttttca agatagtatt tttacgattg cagaagcttg gcaaaaagaa ggatatgttt
      601 ctgaaaaatt tattgcttat ttaaaggata aagaaaaagt gacttttcct tggagtatga
      661 ttgatcgaat tacgccaaat ccttcagaga atgtaaagga agatctattg atgatggggc
      721 tcgaggattt agatatcatt cacacaaaaa aacataccaa tattgctgct ttcactaata
      781 ctgaggtagt tcattattta gttattgaag atagttttcc aaatggccgg cctaaattgg
      841 aaaaggctgg tgtgattatg acagatagag agacggttga taaagcagac atcatgaagg
      901 ttacgacaag cttgaatcca cttcatactg ctttggcagt aacaggttgt ctgctaggat
      961 atcaatcaat ttcagcagag atgcaggatg atgatttggt tggtttaatc aaaggaatag
     1021 gttatttaga aggtttacca gtagttgaaa atccaggcat tattgatcca aaacaattta
     1081 ttgatgaagt aattaataaa cgtttgccta atccctatat tccagataca ccacaacgaa
     1141 ttgcaacaga tacttctcaa aaagtagcga ttcgctatgg tgaaacaatt aaaaagtatg
     1201 tcgaaaaaga cggaaatgca gaaaatctaa catttattcc tatcgctatt gctgcttggt
     1261 tacgttattt actggcagtc gatgatcaag gggaaaattt tgtgccaagt cctgatccgt
     1321 tattggtaga gttgcaggat aaattatcag tattgaaatt aggtgaacaa gatccttcaa
     1381 agattcattg tgcggtacag gaaattttag aaaataaaga aatctttggt ttagatttat
     1441 atcaagttgg ggtaggaaag aaaactgaaa aaatcttttg cgaaatgcta gtaggtcctc
     1501 aagcagttcg taaaacaatc aataaatata taacagaagt tggaggaaag taactatgga
     1561 aatgactttt agatggtatg gggcaaatga agaaaaaatc tcactacaag aaattcgtca
     1621 gatcccagca atgaaaggaa ttgttggaac attaatggat ataccagccg gagaggtttg
     1681 gccgagagaa cgaattaaat ctttaaaaga tgaagtcgaa gccaatggac ttaccttgaa
     1741 agttattgag agtgttaatg ttcatgatga tatcaaaatt ggtttaccaa cacgagatca
     1801 atacattgag aattataaac agacgattcg caatttggca gaatttggga ttgaggttat
     1861 ctgttataat tttatgccag tatttgattg ggtaaaatcg gatttggatt atcgtttaga
     1921 tgatgggtct tctacgttag cctatatttc aaaaaatatc cctactgatc cggaggaaat
     1981 tattagagaa gtagaaaatt ctactaataa ttttgaattg ccgggttggg aacctcatcg
     2041 gttatcacaa attaagcaat tattagaagc ctataaagaa gttgatgaag agaaattacg
     2101 agagaacttt gcatattttc tcaaagcaat tatcccgaca tgtgaagaag ttgggattaa
     2161 aatggcagtt cacccagatg atccaccata ttccattttc ggattacctc gtattgttaa
     2221 aaatcgtgac gatttagatt ggttgtgtaa tgtagtggat tctccttcta atggtattac
     2281 attatgtact ggaagtattg cagaacgacc agataatgat gtctatgaaa ttctatctga
     2341 attttgtcaa cgggatcgga ttccttttgc acatgttcgt aatatcaaat atttaagtga
     2401 caatggactc gatttttacg aagcgccgca taaatcagaa tatggttctc tagatatgta
     2461 taagatatta aaaacaatgt acgataatgg atttaatggc tatattcgtc cagatcatgg
     2521 tcggatgatt tggggagaaa ccggtagacc tggttatgga ttatatgacc gagctctagg
     2581 tgcagtttat ttaaatggta tttgggaagc gcttgaaaag ggggcgagta aataattata
     2641 taagggagtt tatttagagg aaaaattttc tcttctaaaa actaacggaa ggaaaggagt
     2701 gtttaaatat gtttttagat aagaactttt tattagagaa tgaaatagct aaaagacttt
     2761 ttcataaatt tgcaaaacac caaccaatta tcgactatca ttgtcattta gacccagcat
     2821 atttgctaga aaataagcaa tacaagaatt tgactcaggt atggttaaat gataaaggag
     2881 ctggagatca ttataaatgg cgattgatgc gagctaatgg tatagaagaa gagttgatca
     2941 gtggtccagg tgatgattat gaaaaatttt tagcttttgt aaaaacagtg gagaaggcgc
     3001 caggtaatcc tatttttgaa tggtctcatt tggaattaca acgttacttt gatattgatt
     3061 tgacaatttc tgagaagaat gcaccagaaa tttggcgacg tgctaacgaa aaaattcaaa
     3121 cggattccta tcgcccgaaa gaattgttgg agatgatgga tgtgaaagcc atctgtacaa
     3181 cggatgatcc tacagatgac ttgtggtatc atactgagct ttctaaacag gataattctt
     3241 tcaaggtatt acctactttc cgtccagata aggtctggag tatcaaatca tcaggctttt
     3301 cgaactatat tttaaaactt agtcaaacag cggctatacc aatcaaagac tttaatgggt
     3361 tgaagcaagc attgagccaa cgagtggatc attttcatca aattggctgt cgattagcag
     3421 atcacggttt gaatacttat ttttatgaaa tagcagatga aaaaatactc gacaatctgt
     3481 taataaaagc tttagcagga aaaacggatt tccatttatc agaaattgca caatttgtta
     3541 caggtattca attacatttg atgtctgagt atacaagata tggttggacg atgcaaatgc
     3601 atttaaatgc tttaagaaat aattcaagta tattaaagga acaaattgga gtagatgtcg
     3661 gtggtgattc gatgggggat caagctagtt tgacacaaaa tgttgtagca ttgttcgata
     3721 ccgctgaaaa gagtgggtgc ttaccaaaaa caattttata ttcattaaat ccaactgatt
     3781 ggcttccact agcaacagca atgcaaagct tccaaggggg tatgattcaa aaaatccaat
     3841 tgggatgtgc ttggtggttc aatgatacag ctgaaggaat gcgtcaacag ttgactacga
     3901 tggcacaaca aagtttgttg tcgaatttta caggtatgtt gactgattct cgaagttttc
     3961 taagctatcc tcgccatgag tattttcgtc gagtattatg tcaattacta agcgagtggg
     4021 ttaaagcagg ccgaattcca gatgatgaaa tgtatcttag tcagatagtg gagaatattt
     4081 gttatcgtaa tgcgtataat tattttaatt tttttgaaat cggaagtaga aaataatact
     4141 tgaaataaaa gcttaaattt attctttgta agttagtaca gctagacaaa gtatgtatcc
     4201 ataaaggaag gagatagata tgaaaaaagt agaaatttta agtaagttag aagcggctgg
     4261 agtcattgcg gttgtacgtg ggaaaaataa ggatgaggcc attaaagcaa taaattcaat
     4321 tattgcgggt ggaattaaag ggattgaact aacttttaca gttcctaatg ctgaagaaat
     4381 aatcaaagaa attgctaaaa aatatgctga tcaaaaagat gtaataatcg gagccggtac
     4441 ggttttagat gcgtttactg ctcgtatcgc aattatggct ggggcagaat atatcgttag
     4501 tccatctttt gatcgacaaa cagctgagat ttgtaattta tatcaaattc cttatttacc
     4561 aggttgtatg acaattactg agataaagga ggcacttact gctggcgtag atatcattaa
     4621 actattccca ggttctgctt ttggtccatc cattgtaaaa gcctatcaag ctccattacc
     4681 acacgtaaat ataatgccta ctggcggagt aagtatagag aacttagaag attggtttga
     4741 agtaggagtt gttgcagttg gtgtaggtgg gaatttattg gcttctgttg aaaaaggtga
     4801 ttttgaagaa gtaagtcgag tagctagtaa atatatagat aagttttatg agataaagaa
     4861 ttattaggag aaaaaacaaa taatactctg aaatgtaaaa aataaatact gactctttac
     4921 gcttcagagt atagtacgta ctttcatcag aatgaaaacg cttaacaaca gaagggagag
     4981 aattgtatgg aaagaaaatt tggtataaga gacaaaatag gatatatgtt tggagatttt
     5041 ggaaacgatt ttactttcat ttttgctagt tcttttttaa tgatctttta tacaaaagta
     5101 ttggggataa gcggagctgc cgttggaaca ttatttttag tagcaagatt tatcgatgca
     5161 gttactgatg taactatggg gcgtattgtt gatagatcga aagcaactaa gcagggtaag
     5221 tttaaaccgt ggattttaag aatgagtggt ccagtcgctt tagcaagttt tttaatgtat
     5281 caaacttcta tgatttcagc tt
//
LOCUS       DACDVT010000120         5229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDVT010000120 DACDVT010000000
VERSION     DACDVT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.122"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(50..559)
                     /locus_tag="IWL52_002526"
     CDS             complement(50..559)
                     /locus_tag="IWL52_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9779039.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(671..1669)
                     /locus_tag="IWL52_002527"
     CDS             complement(671..1669)
                     /locus_tag="IWL52_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9779040.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1870..2454
                     /locus_tag="IWL52_002528"
     CDS             1870..2454
                     /locus_tag="IWL52_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9779041.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(2531..3706)
                     /locus_tag="IWL52_002529"
     CDS             complement(2531..3706)
                     /locus_tag="IWL52_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9779042.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            3788..4168
                     /locus_tag="IWL52_002530"
     CDS             3788..4168
                     /locus_tag="IWL52_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9779043.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            4242..4604
                     /locus_tag="IWL52_002531"
     CDS             4242..4604
                     /locus_tag="IWL52_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779044.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1714 a          905 c          974 g         1636 t
ORIGIN      
        1 aaaagaaaga actcgtctaa atagacgttt tctttctttt tttgttgaat tattgagtaa
       61 ggcgacttct tttttctggt tctttgtgtg ctaatcgact ggcagcagca gcggcgatcg
      121 ctccgacgat atcatctaag aaggtatgga catgaacgcc atcgtggctg tttagttctt
      181 ttaaaatacc aggtttcacc ttgtcgatat aaccataatt cgtaaagccg atcgtaccgt
      241 aaacattcac gattgacaag gctaggatct catcgatacc gtaaaggcct tcgtcatctt
      301 cgataatatg ttgcaacgga ctcatcaatt ttttcttttc tgctaatata tctaattgga
      361 ttccagtgat aatggcgtta tgtacttcac gctttgttag cacgctgttg acactttcag
      421 tacaatccgc caaagtcaaa tcagaaatat agtctttttg taaaaacatg acaagttgtg
      481 cgatatcttc tacagttacg ccacgttcct tcaatagttc tcttgccttt tcttctaaag
      541 tctcagtttt gacgaccata ttattgctcc ccctttattt tttctttctc tatttcatgt
      601 taacggaaca tgaaaaaaat gcaagaaact gctttcgggt ttaacttgaa atgcaaagag
      661 atataaaaaa tcaactagga cgaagatttt tatcgtataa gcttgtgctg tgaccaggct
      721 gagttcgttc ctttggatta atatagtgta aagcgttgtt cactgcagta ggtgcttcac
      781 cgagcccagt agcaatcagt ttcacttttc cttcataggt acagatgtca ccagctgcat
      841 aaacgcctgg aatagaagta gacatatctg attttacagt aatggcattt ctggtactat
      901 caagtcccca agaagaaaga tgttctaaat tagaagagaa tccataattg acgatcagag
      961 aatcaatcat caaatcaatc gtctcgtcac ttttcacttt ttttaaccgg atatctgtca
     1021 gttccccatt attgcctgat aatccatcga tgagataggg agtaagcaag ttgacagatg
     1081 aagatttcaa gcgtgataca ctatgttcgt gcgcacgaaa ttccgggcga cgatgaatca
     1141 agtaaacttc gcttgcaata ggctctaaca tcaacgtcca atcgatagca gaatcaccac
     1201 ctccagcaat agcgactttt tttccagcat acttcatcaa atcattcacg aaataatcca
     1261 atccgtggtt ttcaaatgat tcagcattat ctaaattcaa ttttcttggc tggaatgaac
     1321 catttcctaa agctaaaatg actgctttgg aataatggat gcctttattt gtagtaatct
     1381 cgataacttc gtcttcacga gtaagcgaga gaacttcttc ttttaaatgg aatgtatggt
     1441 taaaagtagt taattgtttt tctaattgat cgattaattc acttgctttt attgctggat
     1501 acccaggaat atcgtaaata tatttttcgg gatacaaagt tgctaattga ccaccaagtt
     1561 gaggtagact gtcaatgatt tttgttttag cttggcgcat tcctgcgtaa aaagcggcaa
     1621 acatccctac aggacctgca ccgatgattg taatgtcata gatttccata aaaattcctc
     1681 ccttttcaca ctatagcaga atttaattga gaatgcttct caaaaatgtt tgaattcgaa
     1741 atcgaaacgg taagttatag aatgtatcta gtgatgaaaa ataaaagaaa acaaatctag
     1801 tatttttacg agaggttagg tattatgtta ttattattat gaagtatgca aatattagga
     1861 ggaaacaata tggcattccc acaactagat ttaaaaagtg aaaaaggacc aaaagctgtt
     1921 atcaaaacaa accgtggtga cattactgtt caattattcc ctgaactagc accgaagacg
     1981 gttcaaaatt tcattgaact ttccaaaaaa ggttattatg atggcgtgat ttttcaccgc
     2041 gttattcctg atttcatgat ccaaggcggc gatccaactg gaacaggaat gggtggcgaa
     2101 agtatttatg gcgaaagttt tgaagatgaa ttcagtcgtg aactgttcaa ccttcgcgga
     2161 gctttatcta tggcaaattc aggtccaaac acaaacggaa gccaattctt tattgtaaat
     2221 aacacaaatg tgccagctaa tatgcttgga caactagaag gtgctggatt cccatcagaa
     2281 atcattgaag cgtataaagg tggcggaact ccttggctag atttccgaca tacagtcttt
     2341 ggacatgtac tagaagggat ggacacagta gatgaaatcg caaatgtaca acgtggtcca
     2401 caagaccgac ctgtacatga tgtagtgatt gaaacaattg aaatttcaga atagtttggt
     2461 atggtacgga gtaattatga aaaaggacga aacctagtaa ggtctcgtcc ttttattttg
     2521 aataaatgat ttaatcgatt gtctctaaag catacttgat tcgattaagt ccttccagta
     2581 aagtttcttc tggacaagca atattcaagc gcatatgccc atgaccggaa ggaccaaacg
     2641 tgatgccagg atttaatacg acttttcctt tccgaacaag agtgttctcc aattcttggt
     2701 cgtctgaaat ataggcggta aaatctaacc acattaagta agttccttca gggttcatta
     2761 ttctcacttt cggaagatac tgttcaaaaa actgattaac tgtttgaaca tttttttcta
     2821 gataaggaat cagttggctt aaccattctc cacccgtctc ataagccgct tgggttccaa
     2881 tcagtccaaa agtgttgatt tcgtgctgtt ggttcatcac taaatgtttt tcaaaatcag
     2941 ctttcatctc tggatttttg ataaatacca ttgaattttt gattgcagct agattgaaag
     3001 tcttggttgc ggaagtaaaa gcaactagta gatcatctaa attaggatca atcgttagca
     3061 tggaagtaaa tgtatgatcg aataaagtta aatcttgatg gatctcgtca ctaaacagaa
     3121 tcacttgatg ttttaaacac aattcgctta actgtttcaa ttcttctttg ctccaaacac
     3181 gccctccagg attgtgagga ttgcataaaa tcattgcttt tacattgttt tcttcgattt
     3241 ttttctccat gtcagacaag tccatgatga aatgattatt tttttctagc aaagagcttc
     3301 gaacgatgtg acgttgattt gtttctataa tagaggaaaa tggaggataa actgggtcat
     3361 gaatcaaaat agaatcgcct ggttttgtaa aagtttgtac tgctgcagct aaactggtta
     3421 acacaccact tgtaaaaacg atattttctt tcgtcaactc cacagcatgg tgttggcgtt
     3481 cccaatgaat gatagcctga tatagtttat cagacagagt ggcataacca aagattccat
     3541 gtttgatgta ttcactaaac gctttagcta ccccaccggg agacaagaag tccatatctg
     3601 ctacccacaa aggaagtaag tcatccattt gatatgtctc agcaattgcg tcccatttta
     3661 cactttctgt atcttttctt gaaatctgtt tatcaaattc gatcatctta agacctccag
     3721 tcattttcgt cgcttctttt tttgagctat ttacagtata atgataaatg cgaagagagg
     3781 gaagtagatg acttataaaa ttggacaaat aattgaagga aaagtgacag ggatacagcc
     3841 ttatggcgca ttcgtttctc ttgataacga gacgcaagga cttattcatg tttcagaagt
     3901 acaatccggt tatacgaaaa atatccattc attgttaaaa gtcggacagc cagtaacagt
     3961 tcagatcatc gacattgatg aatattccaa aaaaatcagt ttatctttaa gaacgttaga
     4021 aaaaacaaat ccagctgttc catatcgcag aaaaaggtat ttcacgaata aaaatcgtaa
     4081 gatcggattt accactattg cggagcagtt acctatttgg atagaagaag cattatctga
     4141 actagaacgg aaagaaaata agaaataaaa agtgtatttt ttctgaaaat gtgctagaat
     4201 ttttatgtaa gccatagaac aagttgatcg aggtggaaat tttggcaagc aaaagagttg
     4261 ctggtacaat tatgttgcat ttggaggatg gtactaaaaa atttttggtc cattcaattg
     4321 atgacaagtt ggaatttgcg ttagcggaac tttctgaagg aaagactggt ttagcgaata
     4381 tacttaattt cttaaaagaa atagttcata tcgatgttag caagattagt ttgatggaat
     4441 tgacaaatac acatattaaa gaggaaaatg ttccgttgtt tgtctttgaa actgaacaaa
     4501 agaatcaaag agaagaatta ggcgaaggat atatttggga agaaccaggt caaatgcgct
     4561 ctgttcttgg aacttacgaa gtagagggag tgcctttctt ctaaaataca gattaagcga
     4621 acaaagaagt ttgaattttt ttcaaacttc tttttttatt gtttggtcca aaaaatacaa
     4681 tgaaatgttc tgctaaagat agttttttag tgcattttat tttcaaaata tgttgaaaac
     4741 ggttgtataa cgaataataa taattgatcg aaaaatcatt tcacaaagta atgcttaatt
     4801 tatagttgac caaggaagaa atacatgata aatttaacag cgttgattct gaagtggatg
     4861 ttatatcaca tttctaaaaa atttaaaaaa tagttattga catgttacta acgacatgat
     4921 aagataactg agtcgttaaa aaagaaccac ttgcgaagct tgattctatc gaaaaaaact
     4981 tcaaaaaagt tcttgacaaa taacaaacga taagttatga taaatgagtt gctgaaagat
     5041 ttcgaaaaac gaaatcgaaa aaaactttta aaaaagagtt gacaatcgct tgtctctctg
     5101 atatgatata aaagttgcta cggcagccaa aaacgaatcg ggaaacactc ccgatgaagt
     5161 agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat
     5221 tcaagacaa
//
LOCUS       DACDVT010000121         5053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDVT010000121 DACDVT010000000
VERSION     DACDVT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.123"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            57..845
                     /locus_tag="IWL52_002532"
     CDS             57..845
                     /locus_tag="IWL52_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9779045.1"
                     /translation="MVLLESMPDGYIYSNILLKLYLRSLKHEGKLMFNDRIPFNSTML
                     ATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSFIGKSSTEADRKRKYRKEI
                     EEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIENVTPSKKSKAKPVRHKYG
                     EYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSE
                     SKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            857..1126
                     /locus_tag="IWL52_002533"
     CDS             857..1126
                     /locus_tag="IWL52_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779046.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1288..1590
                     /locus_tag="IWL52_002534"
     CDS             1288..1590
                     /locus_tag="IWL52_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779047.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1587..1748
                     /locus_tag="IWL52_002535"
     CDS             1587..1748
                     /locus_tag="IWL52_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9779048.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1745..2050
                     /locus_tag="IWL52_002536"
     CDS             1745..2050
                     /locus_tag="IWL52_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779049.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2050..2406
                     /locus_tag="IWL52_002537"
     CDS             2050..2406
                     /locus_tag="IWL52_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9779050.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2366..2611
                     /locus_tag="IWL52_002538"
     CDS             2366..2611
                     /locus_tag="IWL52_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779051.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2632..2721
                     /locus_tag="IWL52_002539"
                     /pseudo
     CDS             2632..2721
                     /locus_tag="IWL52_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            2796..3263
                     /locus_tag="IWL52_002540"
     CDS             2796..3263
                     /locus_tag="IWL52_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9779052.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            3538..3774
                     /locus_tag="IWL52_002541"
     CDS             3538..3774
                     /locus_tag="IWL52_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779053.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            3798..4139
                     /locus_tag="IWL52_002542"
     CDS             3798..4139
                     /locus_tag="IWL52_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779054.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(4132..4311)
                     /locus_tag="IWL52_002543"
     CDS             complement(4132..4311)
                     /locus_tag="IWL52_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP9779055.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            4533..>5053
                     /locus_tag="IWL52_002544"
     CDS             4533..>5053
                     /locus_tag="IWL52_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779056.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRY"
BASE COUNT         1900 a          737 c         1069 g         1347 t
ORIGIN      
        1 acaacaaacg atactactat ttaaaactaa aagagaactt tttcgatagc gacgagatgg
       61 ttcttttaga aagtatgcca gatggataca tttattctaa tattcttctc aaactttatt
      121 taagaagtct gaaacatgaa ggcaaactaa tgtttaatga caggattcca ttcaattcta
      181 caatgcttgc gactattaca agacactctg tgggagttgt agaaaaagca gtacaaatat
      241 ttcgtgattt acaacttatt gaggtattag ataacggagc aatttatatg tctgatatac
      301 aaagttttat tgggaaatct tcaactgaag ctgataggaa gagaaaatac agaaaagaaa
      361 ttgaggaagc aaaacagaat ttaataacaa gtggacaaat gtcggacaaa tgtccggaca
      421 aaactacacc agagttagag atagagttag agaaagatat agagttagag aaagatatag
      481 agaatgtaac gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
      541 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaatagaa ttccctaatg
      601 actaccaaga gcgaatagaa cggctatctg agtattgtga atcatctggt aagacttata
      661 aaaactattt ggcaactatt cgaagctggg caagaaaaga aaaaagtgaa tctaagaacg
      721 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgagtgg gcaatcgacc
      781 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
      841 tctaagaggt gggaaattga aaatcgatta tctagaacta attaatgaaa tagcaaagta
      901 taaaactggt gaggaaatag aaatcctgag agacgtatat gatcaactcg aagaagctgg
      961 aatcgaagga attaagaatg atcgttcgag ttggagtaag ctcagatact atttcgcact
     1021 ttatatcgat acaacacaat taagaaattt agcatatacc aaattactat ttgttgattg
     1081 tgttaagggc ttgcaaaaac atcttaatga acttgagcag gtgtaatcag atggacctaa
     1141 agacatttac agcacagatt gaactaatgc atcaagaagc tttaagacaa agtgtatcgt
     1201 acgaagacaa gtggctcaac acgttccacg gcggacgtga gagcgcactt gatcaagtac
     1261 tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca gcgaattatc
     1321 gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga agtgatgagg
     1381 cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa aatcgcaatt
     1441 tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag tctcacaggc
     1501 ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc taagaagaga
     1561 acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa atgcgtattc
     1621 gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg gtcaatgaaa
     1681 cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc catcgattgg
     1741 aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa ccaagtcaga
     1801 atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg
     1861 gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg atgaccgaat
     1921 tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca aaaacattat
     1981 ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta ggtatgtgga
     2041 ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa aatcatgatg
     2101 aagattcaga aagataaaaa atcaaaagaa cgagctgaat tagttaagtt agaaatggct
     2161 gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa cattgaattt
     2221 tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg gcaaacaaat
     2281 tggagcaaga aacttcatca agatcgtttc aaatttgtag agaagtataa agaaatatta
     2341 gaagagtatt tgaggaggac agcaaatgat aaaaaaactc gttcaattca gcatggattt
     2401 atatgatatc gaatcaggag caacactatc tgtggaatcg gaccatctaa tcataaattt
     2461 tggtggaaag cgccagatta ttttgtgggt agttgatgat gtactgtttc cagaaattgt
     2521 tcatgatttc gaagaatcaa aagcggttga gtttgaaata gtgaaaaaag taatggaatt
     2581 gattgaaaaa tacgaggagg acagcgaatg ataccgaagt ttagagcgtg ggatagagaa
     2641 aaagacagaa tgaaggcagt agctttggtt gaatttgctg aggcgtataa atcaggagaa
     2701 ctcatttcac gtacaaaata aaaaaagtcg gattcctccg actatgaata atatttccga
     2761 cataagtatt atatcataat tgggggaatc agaggatggt acttttcgat gtaaagaaat
     2821 atgagacacc aagcgcgaaa gatgttgata tggaacgcac aaaacataat gtcgctgtgt
     2881 ttctttcagc atatctatca gctagatgta gagtagggca acctcgagaa ccaaaagtga
     2941 cagcatctta ttccttggtt ccaccttcta cagctgatca tatatttgaa gcagaaagaa
     3001 tgctgatcga taaagaagaa gcacaggaag aatttgagta tctgcataaa ttgtttgttc
     3061 gaggctattc agcgatacag catccgcata agcctgatgt gactgaaagg cgcaagaaga
     3121 tattctatga tcgttatatc aatggtctgc ccatttatgt aactgctcaa aggaataata
     3181 ctagcgaaga atcggttaaa gtagaatcaa acagaattat catccaattt gcttcatcgt
     3241 tagaactggt tgctttcaag tagccagttt ttacactttt tatacccttt tattaccaat
     3301 ttggtttcca ttttatacct tttttatacc aatcacttac ctattcaacg ttgtattatg
     3361 atagtgtcga aagattagga aacaggatcg acaaaataaa atgtaaggga ggaaatctcc
     3421 ctcatcgttt aattaagctt cgataggcag cagcggaaat attaagaata aggatgtgaa
     3481 tttcaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttaatttatg
     3541 tattggaggg tatataaaat gaagatcgct tccgaaaaaa ttgatcaact aacaatttta
     3601 gtaaaccaaa gaatcgaaga tattatcaaa gaattcgaag gtgaaaagat taagaacata
     3661 aagcttactt taatggatca taagttcgaa ttaaattcga caaaaaggaa gatcgttgag
     3721 cttatagaag aagaaatagc tctccagatc aatgagcaac aattagctaa ttaatttatg
     3781 tattggaggg gaaatgaatg gaagtcgaac aaataaagta tcaaggtatg gatcgggaac
     3841 ggttgattga tattattgag caacaacgtt tagaattgat ccaaaaagaa gctgtatgta
     3901 aagagtacaa aaaacatttg gaacaagtaa ttgagtatca ttcagtagaa aagtacagat
     3961 cagtggtgca aaaaaataga gcaacgagtc ctgattctcg tcaagatgta tcgattagta
     4021 tcaagacgcc agtatttaat tctgttaaat cagaaaagct agaaagtatc tcaactgctt
     4081 tggtagaaaa agcagctgaa atgaactccc tagcaaatga agttagtcgt cttatttagt
     4141 catatcgata acttgagagt ctttggtgat tccgtttttt atagaattaa tggtttgttc
     4201 agcggaatat ttagtcaagt atgtttcgct agttgctact acttcattgt tattggactt
     4261 aataacgaag taatattggc cattagttgc ttctcttata acaaagtaca aatttttcac
     4321 cacctataat ttatttcagc ggaccactcg ctgataatta aaattataag ctatgtataa
     4381 aatttcacaa tatcaatttg tcgctgtggc ggaaagggta gacgcaataa aggatgaatg
     4441 ggaaaagtca atgagtaatt atctgaaatt tacaagtccc ctgtttagtt atctaagatt
     4501 atttgataat attatttctg aggtgataga aaatggtggg cgtagttaaa tttcttgggt
     4561 ttacatttga tggatatggt acgttaggcg aatgggtagg tattattttg atagtgatta
     4621 caattcaaca aacatcaaag tactactggt attctaatag ttctaaaatt gtatcaagaa
     4681 taactgaagg aataaccaaa aaagatatta ctgtcgtcgg taaatatggg tttactataa
     4741 ctttattaaa tgaagggaaa tctgaaaccg caataaagtt ttgcgggatt ataggtaaat
     4801 ataacatttt ccaacgtttt atcagaaagc attggtacaa atatatttac aaataccctg
     4861 tagttaaaaa gcttattgaa aaatatgtgg accacgaaat atcttatctc aatccaacag
     4921 accttattaa tcaagaattc cagaatattg gatacttaga aaaaggtcta aaaataccaa
     4981 ttgtaaatga aagtttagtt actgcaatgg tagctaacat aaagaacagc aagaaattag
     5041 aaaagagata taa
//
LOCUS       DACDVT010000122         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDVT010000122 DACDVT010000000
VERSION     DACDVT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.124"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWL52_002545"
     CDS             25..696
                     /locus_tag="IWL52_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9779057.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWL52_002546"
     CDS             755..1195
                     /locus_tag="IWL52_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779058.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWL52_002547"
     CDS             1207..1875
                     /locus_tag="IWL52_002547"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9779059.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWL52_002548"
     CDS             1939..2562
                     /locus_tag="IWL52_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9779060.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWL52_002549"
     CDS             2618..3004
                     /locus_tag="IWL52_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9779061.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWL52_002550"
     CDS             3217..4608
                     /locus_tag="IWL52_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9779062.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1656 a          830 c         1065 g         1444 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gactt
//
LOCUS       DACDVT010000123         4970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDVT010000123 DACDVT010000000
VERSION     DACDVT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.126"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            526..1977
                     /locus_tag="IWL52_002551"
                     /pseudo
     CDS             526..1977
                     /locus_tag="IWL52_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            2245..2655
                     /locus_tag="IWL52_002552"
                     /pseudo
     CDS             2245..2655
                     /locus_tag="IWL52_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2716..4176
                     /locus_tag="IWL52_002553"
     CDS             2716..4176
                     /locus_tag="IWL52_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9779063.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGNPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4187..>4970
                     /locus_tag="IWL52_002554"
     CDS             4187..>4970
                     /locus_tag="IWL52_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9779064.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGAEQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAEVIAQNGAFDAKLDPNETQATQEAFEDYLKEK"
BASE COUNT         1930 a          818 c         1055 g         1167 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaacga acaaatcgaa
       61 aagatggttg aacaccaaag aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg
      121 cgttcttggt ttaggcaaca agagcgaatc gaaaataaag tgcatagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggctataaca agtacggcgg actggatatg acgatcgaga atattccaag aatcagagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg tctttttcag ccgccacgat ccgaaaatat
      361 caacggaagt tggaaacatt agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat
      421 atcctccctg aagtacaagc gatcattgac agcggacgag taacgcaatg gaaaaaaaat
      481 ccgaccattt attttctgaa aggctatcga aaagtcgctt tagaactgaa tgaaaacggg
      541 gaatttgagg aatcaaaaaa ataccaagcc acgtcggaag aggaaaaagc agctattcgt
      601 gaattacttg aagaggctgc gccaactgat tctctgaagg agaaagaaaa cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggggag
      721 atcgtaacat tcgaattgat cgatcaactt aaaacgttgg ataaccaata cgctgcagaa
      781 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt agatcagatc
      841 cgaatggatc taggggatgg cgtcaacagt aatcaaccga tctatttaga gttagcaaaa
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaaacgg ttaaaaatga acctattacg
      961 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
     1021 gcagaagaga ttaaacaaga agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa
     1081 aagcacactc attctcctga agacgtcaaa gaacttctga actttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatt gaggaacaat ttccaggtgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaaggaaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgcgg ctttgttcga
     1321 gaaacagagg ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca gttgaaacct
     1441 gaagactatc cgaaaatctt cccgaaccgt gtctttgact ttcaattaga tactaaagga
     1501 cagtcagaac ttcaagctgg gattcaagcg gtagccaaca atattgggat acaaattcga
     1561 gacatgtcag agagtgaaat ctatcaaaga gaattaggac aagcacgtgg ggcgtacgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcga caagtattca cgaattagca catgctagaa tgcacaaatt ttcagaatta
     1741 gacaccgcaa ccaaagaact gcaggcagaa atgacttctt atattgtatg caaacatttt
     1801 ggaatggaca cgtcggaaaa ggctattcct tacattgcag catggacgaa gaatggtcag
     1861 actttggatg ataaggaagc cgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc
     1921 gcaaatgaat tcattcaaac catttctacg gaaatcaatc aatacagaga aaaagaatat
     1981 gaccaagaaa aaaaacgacc acaaagcggt aatatagaac tggataatgc aacaaaagaa
     2041 ctacaaaaac aagcaggagt ggttgtctca acttaccgac attggttttc agatcatgaa
     2101 gaaacacgtc cgattttaat aaaagagttt gaaaaactat tgacggtcta taaagagaat
     2161 caacaggtac tgacaaaaga aaacttagga gagaaaaagg aagctttagc aattacagaa
     2221 caattaaacc attacgacaa tcattcttcg gaagatgctt tatatgtagt aagtgataat
     2281 ataagtcaat actattttag aaatagaatg gaagttcctg tatttgagaa acttaaagat
     2341 agatcagaaa tagagaagga gaaaatagtc aaaataggta ctctatggat tgataaaaaa
     2401 gatggaaaag tcatggaaga aaatacaggt cgaacattac acctgaagga agcggcgttt
     2461 attgaagatc ctcagcataa tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatc
     2521 caaacggcgt tatcttcaga agagtttgcg ctaggaaatc cattccgatt ttcacttgaa
     2581 acagtgtcag aagtggccaa acgaaaaaat gaaatctatt atgaacaaca attgtcacaa
     2641 aaagaacgcg tataaaacaa gtaaaaaaga ctatctgaaa aacggatagt cttttttctt
     2701 ttgggaggtt tgaaaatgac cgagaaaaaa atgagtctca ttgatcgatg taaacagatc
     2761 gatatagtgg attttgctcg aaacaatgga atggctgtag tcaataaagg acgagactat
     2821 cgcttagaag accatgattc atttgttttt gatcgaagaa aacaacgttt ttattggaac
     2881 agtcaaaata ttagtggcga cattattgaa ttagctaaac tttttttcat agacaaagag
     2941 attcaagatc ctaagcaaca atttaaagca gctactgatt ttattctaaa gaatgaagac
     3001 aaaacagaac gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt
     3061 gattatcaac cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactgccc
     3121 gattggttga ttgattatgc agaaaaagaa ggattgattg ccgaactaaa gccaaaacat
     3181 gaacggcaaa atttcttagt tcgagacgat cgtttagatc atgcggtcgc ctttttatgg
     3241 aaagatccac agacaaaaga gacagtaggg gctagttatc aaggaacgtt cattgattat
     3301 gaacgttttg gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat
     3361 ggatttaacc taaagatcgg taaccctaaa cagttaaaat tttttgagag tagtattgat
     3421 ttacttagct atgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg
     3481 gaagggttaa aacaccatgt gattagtcat tattttggtg aggcagtatc tgaattgaga
     3541 aagaaacaag cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat
     3601 attttctacg aaaaagaaca attaatgggc gcagtcgatc catttacgaa tcagaaagta
     3661 cgttgtgaac gaggaatcgc taatgattgg caagtgccaa aagaatacaa aatcatttat
     3721 gaagaagtcg caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca
     3781 gaaaacaatc tgcaattaac gaaccaactg gtttccgctc acaaagtaaa tgcgtctttt
     3841 ggccagcagc tttctgttaa cgattcaata gaagccatta atttgaaaga tatttgtaga
     3901 gaagtcgcaa aagaattaaa agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt
     3961 ttttatcaag aaaaagggga tattaatgcg cagattcttt tctcttataa agcagaacaa
     4021 tactacaaag gctataaaaa tcatgaacat gaatttgtac ctgaagtaaa aaaggactgg
     4081 aacgatcaat tgaagcatga gatccagcaa caagaaatca gaaagcaaaa acgtgccatg
     4141 ttgtttcaac aaggcagaca acaagaaagg gagtgaaatc gtcatgatga aaaccgttat
     4201 tttagcagaa aagccgtcac aagcgaaagc ttatgccgat agcttctcta aagccactcg
     4261 aaaagacggg tattttgaaa tccaagatcg attgtttcct ggtgaaactg tgatcacgta
     4321 tggttttggt catttagtcg aattagattc gcccgatatg tacgatgaaa attggaaaca
     4381 atggtcatta gaacatttgc ctattttccc tacacattat cactatcatg ttcctaaaga
     4441 taagaaaaag caattcaagg tggtgaaaca gcaacttcaa tcggccgaca ccattattat
     4501 tgcgacagat agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagcagg
     4561 cgcagagcag ggaaagacct ttaaaagact ttggatcaac tccttagaaa aagaagctat
     4621 ttatcaagga tttcagcagt taagagacgc aggagaaacc tatccgaaat ttgaagaagc
     4681 acaagcacgc cagatcgctg attggttgat cggcatgaac ggcagcccac tgtatagttt
     4741 actcttgcag caaaaaggca taccaggaag tttttcctta ggcagagtcc aaaccccaac
     4801 gctttacatg atttatcaat tgcaggaaaa gattagaaat ttcaagaaag aaccttattt
     4861 tgaaggaaaa gccgaagtaa tcgctcaaaa tggcgcattt gatgcgaagc ttgatccgaa
     4921 tgaaacccaa gcgacgcaag aagcatttga agattattta aaagaaaagg
//
LOCUS       DACDVT010000124         4906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDVT010000124 DACDVT010000000
VERSION     DACDVT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.127"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..321
                     /locus_tag="IWL52_002555"
     CDS             <1..321
                     /locus_tag="IWL52_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779065.1"
                     /translation="IFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASAD
                     QLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKL
                     DFVN"
     gene            complement(347..787)
                     /locus_tag="IWL52_002556"
     CDS             complement(347..787)
                     /locus_tag="IWL52_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9779066.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            947..1453
                     /locus_tag="IWL52_002557"
     CDS             947..1453
                     /locus_tag="IWL52_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9779067.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1621..3534
                     /locus_tag="IWL52_002558"
     CDS             1621..3534
                     /locus_tag="IWL52_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9779068.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3539..4540
                     /locus_tag="IWL52_002559"
     CDS             3539..4540
                     /locus_tag="IWL52_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9779069.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4742..>4906)
                     /locus_tag="IWL52_002560"
     CDS             complement(4742..>4906)
                     /locus_tag="IWL52_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9779070.1"
                     /translation="LILFAIHLCSQPYINNNGILVEPAFFCLPLGFLSLLASAGFGFV
                     AFFKRSKQQI"
BASE COUNT         1805 a          756 c          999 g         1346 t
ORIGIN      
        1 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
       61 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
      121 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
      181 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
      241 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
      301 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
      361 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
      421 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
      481 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
      541 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
      601 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
      661 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
      721 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
      781 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
      841 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
      901 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
      961 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     1021 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     1081 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     1141 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     1201 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     1261 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     1321 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     1381 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     1441 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     1501 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     1561 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     1621 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     1681 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     1741 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     1801 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     1861 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     1921 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     1981 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     2041 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     2101 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     2161 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     2221 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     2281 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     2341 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     2401 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     2461 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     2521 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     2581 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     2641 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     2701 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     2761 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     2821 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     2881 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     2941 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     3001 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     3061 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     3121 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     3181 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     3241 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     3301 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     3361 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     3421 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     3481 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     3541 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     3601 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     3661 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     3721 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
     3781 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
     3841 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
     3901 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
     3961 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
     4021 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
     4081 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
     4141 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
     4201 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
     4261 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
     4321 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
     4381 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
     4441 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
     4501 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
     4561 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
     4621 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
     4681 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
     4741 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
     4801 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
     4861 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaa
//
LOCUS       DACDVT010000125         4845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDVT010000125 DACDVT010000000
VERSION     DACDVT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.128"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="IWL52_002561"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="IWL52_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9779071.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="IWL52_002562"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="IWL52_002562"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9779072.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="IWL52_002563"
     CDS             2594..3379
                     /locus_tag="IWL52_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9779073.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="IWL52_002564"
     CDS             3392..4243
                     /locus_tag="IWL52_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9779074.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="IWL52_002565"
     CDS             complement(4367..4819)
                     /locus_tag="IWL52_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9779075.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1635 a          796 c         1021 g         1393 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttc
//
LOCUS       DACDVT010000126         4652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDVT010000126 DACDVT010000000
VERSION     DACDVT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.129"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..600
                     /locus_tag="IWL52_002566"
                     /pseudo
     CDS             1..600
                     /locus_tag="IWL52_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
     gene            634..2337
                     /locus_tag="IWL52_002567"
     CDS             634..2337
                     /locus_tag="IWL52_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9779076.1"
                     /translation="MKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQT
                     VKNEPISKVQNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKE
                     LLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVK
                     GFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIF
                     PNRVFDFQLDQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFT
                     GEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMD
                     TSEKAIPYIATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPE
                     IQPAEVKQDEKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQ
                     HNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKER
                     V"
     gene            2398..3858
                     /locus_tag="IWL52_002568"
     CDS             2398..3858
                     /locus_tag="IWL52_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9779077.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3869..4312
                     /locus_tag="IWL52_002569"
     CDS             3869..4312
                     /locus_tag="IWL52_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAP9779078.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQ"
     gene            4388..>4652
                     /locus_tag="IWL52_002570"
     CDS             4388..>4652
                     /locus_tag="IWL52_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9779079.1"
                     /translation="MIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIR
                     NFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEK"
BASE COUNT         1777 a          776 c          998 g         1101 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaatga tcaaatcgaa
       61 aagatgtttg aacaccaaag aaagaccaac ggtcaaccaa tgaacgataa gagaaacggg
      121 cattcttggt ttaggcaaca agaacgaatc gaaaataaag tccaaagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggttataaca agtacggtgg cctggatatg acgatcgaaa atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ctgccacgat ccgaaaatac
      361 caacgaaaat tggaaacatt agaacagctg aaagaaaaat cagagaaagg aaaagaaaat
      421 attcttcctg aagtacaagc gatcattgac agcggacgag tgacgcaatg gaaaaagaat
      481 ccaactatct attttctgaa aggctatcga aaagtcgctt tagaactcaa tgaaaacggg
      541 ggatttgagg aatctaaaaa ataccaagcc acgtcggaag aagaaaaggc agctgttcgt
      601 gaattacttg aagaagctac acctactgat tctgtgaagg agaaagaaac cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gcaaaagggt tgaaaagtta tggtggggag
      721 atcgtaacac tagaattgat cgatcaactc aaaacactgg ataaccaata cgccgcagaa
      781 gcaggctatt ataaattcta ttttgaagaa gtgaagaatg gagaagtagt agatcagata
      841 cgaatggatc taggggatgg cgtcaatagt aatcacccca tctatttaga gttagctaga
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaaacag tcaaaaatga acctatttca
      961 aaagtacaaa atgagattaa agagacaaaa acaaaacaaa agggttatca aaaacgaacg
     1021 gcagaagaga ttaagcaaga agtcaaatca ctttcccaac aggcgcttga tgcggtgaaa
     1081 aagcatactc attcacctga agacgtcaaa gaacttttga attttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatc gaggaacaat ttccaggcgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaacagagg ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca attgaagcct
     1441 gaagactatc cgaaaatctt cccgaaccgc gtctttgatt ttcaattgga tcaaacaggg
     1501 catacagaac ttcaagttgg gattcaagcg gtagccaaaa atattggaat tcaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgtgg ggcgtacgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcca caagtattca cgaattagcc catgctagaa tgcacaaatt ttcagaatta
     1741 gatactgcaa caaaagaact acaggcagaa atgacctcct atattgtgtg caagcatttt
     1801 gggatggaca cctcggagaa agctattcct tatattgcga catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc tgcggaacgt gggaaaatca tgaatgatgt cagtcgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccagc cagcagaagt gaagcaggat gagaaaaatg ttgtagaaac agcagaacaa
     2041 aagaatattg tgctcgttgg ctcactttgg atcgacaaga aagacgggaa agtcctggaa
     2101 gaagacacgg gccgaacgtt acatttgaag gatgcggcgt ttattgaaga tacgcagcat
     2161 aatcaagtga ttttaaaagg aaaaagttat ggaaaagaga ttcaaacggc gttaccttca
     2221 gaagaatttg cgctaggaaa tccattccga ttttcacttg aaacagtgtc agaagtggcc
     2281 aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac aaaaagaacg cgtataaaac
     2341 aagtaaaaaa gactatctga aaaacggata gtcttttttc ttttgggagg tttgaaaatg
     2401 accgagaaaa aaatgagtct tattgatcga tgtaaacaga tcgatatagt ggattttgct
     2461 cgaaacaatg gaatggctgt agtcaataaa ggacgagact atcgcttaga agaccatgat
     2521 tcatttgttt ttgatcgaag aaaacaacgt ttttattgga acagtcaaaa tattagtggc
     2581 gacattattg aattagctaa actttttttc atagacaaag agattcaaga ctctaagcaa
     2641 caatttaaag cagccacaga ttttattcta aagaatgaag acaaaacaga acgagtagaa
     2701 aatcttcatt ttgagacaga aaagtacaaa gatcaccccg ttgattatca accactcaca
     2761 gaaaaaggaa ggaattattt gaaggaagaa cgaaaactgc ccgattggtt gattgattat
     2821 gcagaaaaag aaggattgat cgccgaacta aagccaaaac atgaacggca aaatttctta
     2881 gttcaagacg atcgtttgga tcatgcggtc gcctttttat ggaaagatcc acagacaaaa
     2941 gagacagtag gggcgagtta tcaaggaaca ttcattgatt atgaacgttt tggtgagaga
     3001 ggcacatata agcatataga taaaaattcg acagcgaatc atggatttaa cctaaagatc
     3061 ggtgacccta aacagttaaa attttttgag agtagtattg atttacttag ctatgctgca
     3121 ctgaatcgtg atcagttaaa cgatacgtgg ttagtctcta tggaagggtt aaaacaccat
     3181 gtgatcagtc attattttgg tgaggcagta tctgaattga gaaagaaaca agcctttcct
     3241 caatccattg agatttgtgt ggataatgac cgtgcaggac atattttcta cgaaaaagaa
     3301 caattaatgg gcgcagttga tccatttacg aatcagaaag tacgttgtga acgaggaatc
     3361 gctaatgatt ggcaagtgcc aaaagaatac aaagtcattt atgaagaagt tgcaaaagaa
     3421 atgaaagttg aacctgaagc aattatggcc atacacaaga cagaaaacaa tctgcaatta
     3481 acgaaccaac tggtttccgc tcacaaagta aatgcgtctt ttggtcagca gctttctgtt
     3541 aacgattcaa tagaagctat taatttgaaa gatatttgta gagaagtcgc aaaagaatta
     3601 aaaggttgtg aacgcgtgga tggtacgtac gattttgatc gtttttatca agaaaaaggg
     3661 gatattaatg ctcagattct tttctcttat aaagcagaac aatactacaa aggctataaa
     3721 aatcatgaac atgaatttgt acctgaagta aaaaaggatt ggaatgatca attgaagcat
     3781 gaaatccacc aacaagagat cagaaagcaa aaacgtgcga tgttgtttca acaaggcaaa
     3841 caacaagaaa gggagtgaaa tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc
     3901 acaagcgaaa gcttatgccg atagcttctc taaagccacc cgaaaagacg ggtattttga
     3961 aatccaagat agattgtttc ctggtgaaac tgtgatcacg tatggttttg gtcatttagt
     4021 cgagttagat tcgcccgata tgtacgatga aaattggaaa caatggtcat tagagcattt
     4081 gcctattttc cctacacatt atcactatca tgttcctaaa gataagaaaa agcaattcaa
     4141 ggtggtgaaa cagcaacttc aatcggccga caccattatt attgcgacag atagcgatcg
     4201 tgaaggggaa ctgatagctt ggacgatcat tcaacaagca ggcgccgatc atggaaagat
     4261 atttaaaaga ctttggatca actccttaga aaaagaagcc atttatcagt gattccagca
     4321 attacgagac gcagaagaga cctatcccaa atttgaagaa gcacaagcac gccagatcgc
     4381 tgattggttg atcggcatga acggcagccc actgtatagt ttactcttgc agcaaaaagg
     4441 cataccagga agtttttcct taggcagagt ccaaacccca acgctttaca tgatttatca
     4501 gctgcaggaa aagattagaa acttccaaaa agagccttat tttgaaggaa aagcccaagt
     4561 aatcgctcaa aatggcgcat ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca
     4621 agaagcattt gaagattatt taaaagaaaa gg
//
LOCUS       DACDVT010000127         4635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDVT010000127 DACDVT010000000
VERSION     DACDVT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.130"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IWL52_002571"
     CDS             <1..391
                     /locus_tag="IWL52_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9779080.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQIF
                     NTELFEEYTYEVTGNIYENSELLEEQR"
     gene            388..945
                     /locus_tag="IWL52_002572"
     CDS             388..945
                     /locus_tag="IWL52_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9779081.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            942..1238
                     /locus_tag="IWL52_002573"
     CDS             942..1238
                     /locus_tag="IWL52_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779082.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1315..1728
                     /locus_tag="IWL52_002574"
     CDS             1315..1728
                     /locus_tag="IWL52_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9779083.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(2186..2461)
                     /locus_tag="IWL52_002575"
     CDS             complement(2186..2461)
                     /locus_tag="IWL52_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779084.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            2915..3121
                     /locus_tag="IWL52_002576"
     CDS             2915..3121
                     /locus_tag="IWL52_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779085.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            3317..3484
                     /locus_tag="IWL52_002577"
     CDS             3317..3484
                     /locus_tag="IWL52_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9779086.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            3510..3854
                     /locus_tag="IWL52_002578"
     CDS             3510..3854
                     /locus_tag="IWL52_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9779087.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3859..4140
                     /locus_tag="IWL52_002579"
     CDS             3859..4140
                     /locus_tag="IWL52_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779088.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            4243..4557
                     /locus_tag="IWL52_002580"
     CDS             4243..4557
                     /locus_tag="IWL52_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9779089.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
BASE COUNT         1623 a          698 c          994 g         1320 t
ORIGIN      
        1 acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa
       61 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
      121 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat
      181 agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa
      241 aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca
      301 aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga
      361 gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa
      421 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca
      481 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg
      541 gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta
      601 atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata
      661 gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca
      721 ctattgagcc tatacaacgg ctacgaggtt gagaaagagc cgttatgggc aataaagaat
      781 gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt
      841 tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca
      901 gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat
      961 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
     1021 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
     1081 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
     1141 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
     1201 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
     1261 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
     1321 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
     1381 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
     1441 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
     1501 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
     1561 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacagaatt
     1621 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
     1681 gagtttgcag aagcatataa acacggaaaa ataattgctt ataaataatt tggcggtttt
     1741 ttggcggaat gatggcggtt tttagacatt taccagtgtt attatggtag tgtcgaaaga
     1801 ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat ctccctcatc gttttaaact
     1861 tcttcttaga tagacagcaa ctaattatga agaaaaggat gtgaattcaa ctcctcctaa
     1921 attcttcatc ggttgctgtt tattttgatt agacagcagc actcacaaaa ctaaacatgt
     1981 tcttgtcgtc tttcaactgc tgctgtctat taatttatgt attggaggga agaaaaatga
     2041 aattatcagt agaaggcact ccagaagaaa tacaagaatt gctccaagct attggtagta
     2101 gcaaggagca aaaggtaagt gtttctaatt cagatattga cggtatttgc aattgtttaa
     2161 acagagaaaa aatatctaat ccttactaag agaaaatatg ttcgttaatg aatttccaat
     2221 tttcctctgt agctctacct tgataatttt tttgcagttc acagatgaat aaggaatcgt
     2281 tattatcaag tactgtattt aatttttcac acatttgatt tggattcaaa ttagttttaa
     2341 ctaaccaaaa agatttttgt agctttatat atgccccaaa ttcttttatt atttcaaata
     2401 ctttatcata cttttgccct gggttattaa gatcataact aatcatgtag ggtttataca
     2461 tgttgtcttg cacatccttg ttttatattt cagcggacca ctcgctgata aataaaatta
     2521 tacgcttagt atttattttc acaatattaa tttatcgtaa taacttttga gtaaatagaa
     2581 caaaaaacct gcactagttt ccgctagtac aggcagtgac tatatcggtt gataatctag
     2641 catatattaa aaataattgc aagaaaagaa atttatagtg gtttatacca aattatcaaa
     2701 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
     2761 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
     2821 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
     2881 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
     2941 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
     3001 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
     3061 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
     3121 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
     3181 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
     3241 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
     3301 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     3361 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
     3421 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     3481 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
     3541 agttgacaag agaagatcgt ttggttaagt tctatcagtc tcctaaatgg cgcaggctta
     3601 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
     3661 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     3721 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     3781 atcttacagc attagataaa caagagaaga agatagaaag ctttgctaac ttcgatgcaa
     3841 gtgaaaggtg gtaagtgcat gatcatcaat gataatggca gagagtatga tacagaaaag
     3901 attgaagagt attcatctta tactcaggga ttaattaaac gtttgatata cgttcgctat
     3961 gtaggtatta gggatctgtt atcagataac tgttgtagta aatacaaagt gaatcaagta
     4021 agagaagcgt tgaataaaga taataacgtc gaaagaataa aaaatgtttt tggatatagt
     4081 attgaagaga ttaattatta cattgacttc gctgaagctt tcattccgat ggtgagataa
     4141 ccccccctta aaataaatcg caaatttttt gggggtgatg aaacggaggg ggctgtcagg
     4201 aaaagagatt ttttcgaact ttatcatgaa aggagggcta aaatgtttaa aaacgaattg
     4261 tctcaaaatc gctacagaga aaaattacgc cgctctttaa taagccaatt ggaaagtcag
     4321 aaaacaaata ttgagccatt cttagataat gttgatcgtt atatcagttt atgggaaacg
     4381 gcgatatcac tggaagaaga tatatccgag aacggcatta gactggagaa tggtaaaaag
     4441 aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa tgggattgat gttggataaa
     4501 cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat caattcctga gttataagca
     4561 tattgaaaat tggttcaaag ctatagaaga aggcactatc aaggtatgca aagagcaatt
     4621 attgctaaaa aatta
//
LOCUS       DACDVT010000128         4623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDVT010000128 DACDVT010000000
VERSION     DACDVT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.131"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(52..522)
                     /locus_tag="IWL52_002581"
     CDS             complement(52..522)
                     /locus_tag="IWL52_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9779090.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IWL52_002582"
     CDS             complement(681..1139)
                     /locus_tag="IWL52_002582"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9779091.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IWL52_002583"
     CDS             complement(1310..2455)
                     /locus_tag="IWL52_002583"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9779092.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IWL52_002584"
     CDS             complement(2468..3385)
                     /locus_tag="IWL52_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9779093.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IWL52_002585"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IWL52_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9779094.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYGTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1448 a          844 c          875 g         1456 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcatctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acggcacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaa
//
LOCUS       DACDVT010000129         4583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDVT010000129 DACDVT010000000
VERSION     DACDVT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.132"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            832..1566
                     /locus_tag="IWL52_002586"
     CDS             832..1566
                     /locus_tag="IWL52_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9779095.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IWL52_002587"
     CDS             1582..2751
                     /locus_tag="IWL52_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9779096.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IWL52_002588"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IWL52_002588"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9779097.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..>4583
                     /locus_tag="IWL52_002589"
     CDS             3841..>4583
                     /locus_tag="IWL52_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9779098.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQ"
BASE COUNT         1556 a          766 c          987 g         1274 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattta
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca aga
//
LOCUS       DACDVT010000130         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDVT010000130 DACDVT010000000
VERSION     DACDVT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.133"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWL52_002590"
     CDS             574..1008
                     /locus_tag="IWL52_002590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779099.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWL52_002591"
     CDS             2298..3221
                     /locus_tag="IWL52_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9779100.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWL52_002592"
     CDS             3237..3776
                     /locus_tag="IWL52_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9779101.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1668 a          671 c          755 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDVT010000131         4427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDVT010000131 DACDVT010000000
VERSION     DACDVT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.134"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..853
                     /locus_tag="IWL52_002593"
     CDS             <1..853
                     /locus_tag="IWL52_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9779102.1"
                     /translation="ILLSVIPSLVQQISSLASNMPDFIKQVESWLKEVAELPLFKEVD
                     INKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASATVVIITAPFILFYMLKDGEKL
                     VPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIECLFVGTFTFLGYMVIGVDYAF
                     LFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKALLCCVVVLIVQQLDGNIIYPNV
                     IGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAICKTIISYIIKIVKEDKQNENK
                     KKIATTS"
     gene            981..2357
                     /locus_tag="IWL52_002594"
     CDS             981..2357
                     /locus_tag="IWL52_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9779103.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            2758..4338
                     /locus_tag="IWL52_002595"
     CDS             2758..4338
                     /locus_tag="IWL52_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9779104.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT         1447 a          738 c          935 g         1307 t
ORIGIN      
        1 gattttactt agtgtcatcc caagtctagt acagcaaatt tcctcattgg catctaatat
       61 gcctgatttc atcaaacagg tagaaagctg gttaaaagag gtagccgaat tgcctttgtt
      121 caaggaagta gatatcaata aatattttga gcaattagac atttcttacg ggacaatcat
      181 tcagcaattc cttagtggag tatccaatag tttaggttcg atcgttggaa cgatcgcttc
      241 ggcaaccgta gtaatcatta cagcaccatt catcttgttc tatatgttga aagacggaga
      301 aaaactagtg ccaaacatcc aacagttttt cccagaaaaa agaagaaacc aaattgtgga
      361 gttattgggg caactgaata aaacattggc taattacatt agcggacaag caatcgaatg
      421 tttattcgtt ggtaccttca catttttagg atatatggta attggtgtgg attatgcttt
      481 cttatttggg gtcattgcgg gattgacaaa cctaattcca tatttaggac cttatctagg
      541 actagcacct gcatttctag taacagtctt caatgatccg gtaaaagcat tactttgttg
      601 tgtggttgtg ctaatcgtcc aacagttgga tgggaatatc atttatccta atgtaattgg
      661 gaaatcactg aaaatccatc cattaacgat cattatcgtt cttctagtag ctggaaatat
      721 tgccggattg cttggtatct tcttaggtgt tcctttttac gcaatctgca agacgatcat
      781 ttcctatata atcaaaatcg tcaaagaaga caagcaaaat gaaaacaaga agaaaattgc
      841 tacgacctcg taggtagttt tagtacactt gtttttattg caaagaaaat tgctttgtga
      901 taccatttta acgtggctat agtgccacgt ttttttctat cgtaaactaa aaattaattt
      961 tataaggaga gaatgcgatt atgaatgctg accctgagag tcagtcgctg ttagcgcaga
     1021 ttttattatt gatcgttttg acattgatca atgctttttt agcagcttcc gaaattgctg
     1081 ttgtttcgat caataagaat cgaatcgagc aaaaagctga tgaaggagat gtgaaatctc
     1141 aaaagctttt aaaaatattg cagaatccaa ataatttcct atcgacaatc caagtaggaa
     1201 tcactttggt caacattttg tctggtgctt cactagctaa tactttatcg gaacggttag
     1261 ctccggtatt aggtggcggc acagcggcaa aaaatattgc aaatatcatt gtgctggcta
     1321 ttttgaccta tgtatcgatt gtttttggag aattgtaccc taaacgtatt gctttaaata
     1381 aatcagaaga agtcgcaaag ttcacttcag ggctgatacg cttgattggt gtagtagcta
     1441 agcccttcgt ctggctgctt tctgcttcca caagtttatt ggcgcgtatc acgccgatga
     1501 cttttgatga tgaagattca aaaatgacca gagatgaaat gcgttatatg cttgaaaacg
     1561 aaggcgtatt gaataatgaa gaattagaaa tgctccaagg ggttttttct cttgatacga
     1621 aagttgcccg agaagtcatg gtcccacgaa cggatgcatt tatgattgat atcaacgaca
     1681 ctgtggaaga aaatgtgaat gaagttttat ccgaaaacta ttcaagaatc cctgtttata
     1741 acgaagacaa agataaagta gtcggaatcc ttcatacaaa gaatttacta aaagctgccc
     1801 ataaattcgg ctttgataat ttagatatca agaaaatcat gcaggagcct ttattcgttc
     1861 ctgaaacggt ctttattgat gatctgttat atgaaatgaa aaagacgcag aatcagatgg
     1921 ctattttatt agatgaatat ggcggcgtcg ttgggttagc aacactagaa gaccttttag
     1981 aagaaatcgt cggagaaatc gatgatgaat cagatgaagt tgagaatctt tatgaaaaaa
     2041 tcgatgaaca cgaatatatc atccaaggac gtatgctgat tgatgagttc aatgaagctt
     2101 ttgacagtga tctgcatatg agtgatgtag atacgatggc tggttatttg attacagcat
     2161 taggcatgat tccggatgaa ggcgaaaagt tatcgtttga tgtcgataat attacgctgg
     2221 tttctgaaga aatggaaggt tctcgaatcc ttaaaatccg tgtcattttt catgatccgg
     2281 aagaaacaga agcggaacct gaagaagaac gaagatactt cagaaaagaa ttcgaagatg
     2341 acgagccaag aagataattg gaaacttggt tatgaaagac gtgttgcttg aataaacaag
     2401 caacacgtct tttcttttag tcagctgaca aaaaaatatt tgactcttat ggtttggtgg
     2461 ctttttcatt cgctgaatcc atgctatact gattcagttg tagtagttga caaaggttgt
     2521 gaaaaagcta tcctgcatta agtaataaga gcagccaagc aaaaacagct tttcgtattt
     2581 ttggcttggg tgggcttatt gctgataggc tgtctctaga acgccgttta ctcggaaaaa
     2641 gagactgcta cacggctttt gtcacagccc tatatgaaat tgtctttctt taagagatcg
     2701 gacaatctgt gcctagagaa aatataaata attgaatgga agaaatggag ttaggttatg
     2761 aataatccaa aattaaagca acaagtggac aatcgcagaa cttttgcgat catttcccat
     2821 ccggatgctg ggaaaacaac gattacagaa caattactat tgtttggtgg ggctatccgt
     2881 caagccggga ccgtaaaagg caaaaaaaca ggtaattttg ccaaatcaga ctggatggaa
     2941 atcgagaaac aacgtgggat ttctgttact agttctgtta tgcagtttga ttatcaaggg
     3001 aaacgagtaa atatcttgga tacaccagga catgaagatt tttctgaaga tacttatcgt
     3061 actttgatgg ccgtggatag tgcggtcatg gtaatcgata gtgcaaaagg gatcgaagca
     3121 cagacaaaga aattattcca agttgtaaaa aaacgtggca tcccgatatt tacttttatc
     3181 aacaaactag atcgtgatgg tcgtgaacca ttggaattgt tagaagaatt agaagagcta
     3241 ttagatatcg aatcttatcc gatgaattgg ccaatcggta tgggtaaagg gctagaaggt
     3301 ctttatgata tctaccataa tcgtgtagag ttttatcgtc cggaaaatta tcaggatgaa
     3361 cgattagtag aattggatga agatggactg cttcccgaaa atcatcctct aactgaaaat
     3421 tccttatatg aacaagtctt agaagaagta gaactaatca aagaggctgg agatgcattt
     3481 aatcaagaaa aaattgctcg tggcgatcag acaccagtat tttttggttc tgcattgact
     3541 aacttcggtg tacagacttt tttagagact ttcgttgaat tggcaccagc tccttatgga
     3601 cacaaaacac aagatgatca gatggtaagt ccttatgagg aagaattttc aggtttcgta
     3661 ttcaagatcc aagcaaatat gaatccagcc catcgtgacc gaatcgcttt tgtccgaatc
     3721 tgttctggaa cattcgaacg aggaatggat gtctggttgg aacgtacaaa taaaaaactg
     3781 aaattaagca atgtcacaca atttatggca gattcgagag aaaacgtcga aaaagcagta
     3841 gcaggcgata tcatcggtgt atatgataca ggcaattatc agattgggga tacattgttc
     3901 gaaggaaaac taaaagttgc ttatgaagaa ctcccttcat ttactccaga gctgtttatg
     3961 aaagtgactg caaaaaatgt catgaaacaa aaatccttcc acaaaggaat ctatcaatta
     4021 gttcaagaag gagcgattca gctttataaa acatatctaa cggaagaata tatcattgga
     4081 gctgttggtc agctacaatt cgaagtattc caatatcgta tgttaaatga gtataacgcc
     4141 gaagtaatca tgtctccaat gggacacaaa atcgctcgct ggatcgatcc agaagatttg
     4201 gatgagaaga tgagttcaag tcgcaatatc ttggctcgtg accgtttcga ccaaccgttg
     4261 ttcttattcg agaaccaatt tgcagaacgc tggtttgcgg ataaatatcc caatgtaaaa
     4321 ttaaaaagtt tgatgtaaag aagtcttgac acaagctgcc ctgcatcaag taataagaat
     4381 agccaaacaa aaacagcctc tcatattttg tttagagccg tgacgga
//
LOCUS       DACDVT010000132         4414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDVT010000132 DACDVT010000000
VERSION     DACDVT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.135"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IWL52_002596"
     CDS             complement(37..1257)
                     /locus_tag="IWL52_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9779105.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IWL52_002597"
     CDS             complement(1277..1882)
                     /locus_tag="IWL52_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9779106.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IWL52_002598"
     CDS             complement(1931..2863)
                     /locus_tag="IWL52_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9779107.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IWL52_002599"
     CDS             complement(3284..4144)
                     /locus_tag="IWL52_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9779108.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
     gene            complement(4291..>4414)
                     /locus_tag="IWL52_002600"
     CDS             complement(4291..>4414)
                     /locus_tag="IWL52_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9779109.1"
                     /translation="STTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1254 a          888 c          729 g         1543 t
ORIGIN      
        1 tttattcttt atttaaagct ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa gatatcggtt ctgttgcaaa gttttaaata
     4261 aagaataaaa tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat
     4321 ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc
     4381 tcgaatggct tccatgcctt taatcgtggt agag
//
LOCUS       DACDVT010000133         4194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDVT010000133 DACDVT010000000
VERSION     DACDVT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.137"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..395)
                     /locus_tag="IWL52_002601"
     CDS             complement(<1..395)
                     /locus_tag="IWL52_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP9779110.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFT"
     gene            845..1459
                     /locus_tag="IWL52_002602"
     CDS             845..1459
                     /locus_tag="IWL52_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9779111.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1624..1866)
                     /locus_tag="IWL52_002603"
                     /pseudo
     CDS             complement(1624..1866)
                     /locus_tag="IWL52_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2070..3389)
                     /locus_tag="IWL52_002604"
     CDS             complement(2070..3389)
                     /locus_tag="IWL52_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP9779112.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(3544..4131)
                     /locus_tag="IWL52_002605"
                     /pseudo
     CDS             complement(3544..4131)
                     /locus_tag="IWL52_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1347 a          738 c          708 g         1401 t
ORIGIN      
        1 gtaaaaagat tcagtgatcg ggtcactttt aagattgatt catcgatcga gtaaatcagt
       61 agatcttcat cagccacata tcttctgaaa atattattta cctgcatatt tcgcttgata
      121 tataggttca tacgtggtgg aacaacgtgt agtgttttag gaaatggttg tggtaagtca
      181 cgtggtctac tcacatttgt aataccatac cgcttttttg cttcaggaga agaagctaat
      241 atcaatcctg aaccagtatt atcagctcga ctcataacaa caagttctgt tgttaatgga
      301 tctaaatttc tttctataca ttcgacactt gcgtaaaagc ttttcatatc gattaaaaag
      361 taatcattta ctggctcttt tgaataatcc atcataaata acactccatt ctttcacatt
      421 atacaaacat atgttcgata ttgtaaagaa gaaatagcta aatacataaa aaaagaagca
      481 taggtatgct atcaccctgc ttcttcttat gatttgtatg atcaatttat tctatcactt
      541 tactaattaa tttgaaaatc taataaaaac tctcactcta aaagagtgag agactgcaaa
      601 ttagagtacg gacttttttg gagttccgtt ctttaatatt aacaaatgaa taaaatcctt
      661 acaacatttg agaataagtt atcagtagac acttacaaaa aattctcata ctatttcctc
      721 catcattagt ttttgtctat ataaggttta ccctaaatag acactggtat aacattgaaa
      781 aatatggctt cctcgtgtat gattagggtg tattttaaat agttgcagaa agtaggtaaa
      841 agttttgaga gttgcataca tacgtgtctc atcgatcgat caaaatgaac aacgccagat
      901 tgaagaaatg aaaaaattcg gtgcagagag aattttcatc gagaaacaat ctggcgcaac
      961 gattactcat cgacctgttt tccaagaagc attagattac gtccgagatc aagatatttt
     1021 tattgtggaa gctattgatc gacttggaag aaattatgac gagatcatcg cttcagtcaa
     1081 ttacttgaag aagaaaaatg tgaagctgat cattactagt ttaccgatca tggctgaagc
     1141 aattggcaat cctctcctgg ataaattcat taaggatctg atcatccaaa ttttggccat
     1201 gatcgctgaa caagaacgga ctgaatcgaa acgtcgccag gcacaaggaa ttaagatcgc
     1261 aaaagcaaac ggcgtctaca aagggcggcc caaattatac agtgcagacg caaaagatcc
     1321 ccaacgtcgc cttgtttaca aaagcatcgt tgaagatctt aaaaatggtg ttgcgattgc
     1381 taagattgct aaagactaca atgttacgag acaaaccgtg tatagaataa aaaaagacag
     1441 catggtcaat gacaaatagt tgatcatgct gcttttatta tcttctcatc tttaatttcc
     1501 tacatataat tactatctat aaaattaagt cttactcaga acagataaag cataaaattc
     1561 caacaaacca ttttttactt aactaaataa agagtctgtt aatataatct tcacttattt
     1621 acgttaatcc cctactgtat ttgttggttc cttatctata ttttctacaa aatccttatt
     1681 atcttgtgat ttagcttcgt ataattcttt gtatgtcgac acattggtta tactaattcc
     1741 tatagctagt tcttgagact ctacttttct ctctagctcc tttgacaaag tgatttgaga
     1801 tttatttggt tccgttggag tcaattctat agattcttca taaaaatctc tatacataaa
     1861 gctatccctg aataattcat agctttaatt ctttggcaca ttttattgaa atatgtatca
     1921 ctaatatccc tatcaactga ttttgactaa aaagttactt caaacattca ctgttcaaaa
     1981 atattgagga ataaattttg ggtgtaaggc attttgagca tacttctccc tggataactt
     2041 cccagtaaaa aaatcgttag attgttggat tagatccatt gactggctcg cctcaggcga
     2101 gcaaagaccc tgtagaattc attctgatac acatgataac tagataattt gagatagact
     2161 cgtctacccg tttggactaa tttcccagca actttcagaa acttcaatcg aatagaatga
     2221 atggtcattc ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt gactaagttg
     2281 taagctatca agctgagcat cattcggaca tgattctcca aaaagcgagg actgtctgtt
     2341 ttgtcaaagt aaaagccagc tttcgcttct ttaatgaagt tctccattgt gccacgtttg
     2401 gcatagatag aaaagatggt atcaggagaa acattttctg atagattcgt cacgataaat
     2461 tcatgtcgaa agagtagttc gcccgcttca cgtgttgagc gtatacacac gcgacgactt
     2521 tgtgaccagg attgtgcttg ataagtggtc gagaagtact gaacttctcg ttcttcccac
     2581 ttttgattat cgccataaag tactgatttc tcagctattt gacctagtct acgattattc
     2641 ttcagtcgaa taacataatg actctttttt gattcacacg aatcatacac accaggtgta
     2701 gcgaacccgc tgtctccacg aaccaagatg tcagtgtttg gtagagagtg attatagtgc
     2761 tctaataaag gtgtgagaaa ctcctttacg ccttttgatg tatattgatt tcctgaacgt
     2821 agttcagctt ttaagaagtc accagtcaat ccgtcaaaag ctaccaatgg atggtaacca
     2881 taggtttggt agtgtgtatt ataattcgtt tgttcttggt gaccaaatgt atctgaatgg
     2941 gtcgaatcta aatcaatgat taattccgta tcattacgga tgaggcgtgc tttatcgata
     3001 agttcttggt tcaaagcttg aagttcatgg atattctcct cagatagtcg atctaaaaat
     3061 cgggaaagtg aagattgaga agcgagttct ttcctatcta aaacagcttt aaacacagga
     3121 tcttgtctca ggagattagc tgcagaatca gctgagtaac cagcaattaa ttgcataatg
     3181 aactgctcaa gtatggataa gttgtcatga gtaaaatacg ctcgttcgtc ttcaatgtga
     3241 atgtgttgct tagccagttc agaaaaacca aagatgttca tcagctcttt cactaggacg
     3301 agacccgaat cactcgataa ttgaccacct gtatgagaaa tagtgatatt tgaattgaat
     3361 tttacttggt ttttgtgtaa gctagtcatg aaaagaactc ctttcttttg gttggtttag
     3421 tcactttaac catagcagaa aggggttctt ttttcatcac ttaacgggtg aagatgaaaa
     3481 agtaattgta agctgccgtg aggcagtttc acatggtttt aattaaaagt atgaattatt
     3541 caggctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca tataacattc
     3601 aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt tattacgttt
     3661 ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat taaagtaccc
     3721 tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct caaatattag
     3781 agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac aggctgtaat
     3841 tatcgaaata aacctatctc tagtatttga tatattttta cttcttttaa ggagtataag
     3901 agaaaagggg atactttcgt gtccatgcat gtaattaact aattgttgag cttcagaagt
     3961 tatatctcta attatatagt taacggaatt aggaagtgtc tttaactcta atgaaatttc
     4021 tatccaatta ctatcttcta atctgtatat gctcgaagta taatttgatt cgtcagcaag
     4081 catcgctaca tcccaaacac tgatcataaa atcttttaaa cgttcaatca tttcagcaat
     4141 attgctaaaa tcagctaatt tttctttaga ataaaaaaaa acatttatat caga
//
LOCUS       DACDVT010000134         4154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDVT010000134 DACDVT010000000
VERSION     DACDVT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.138"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(175..1866)
                     /locus_tag="IWL52_002606"
                     /pseudo
     CDS             complement(175..1866)
                     /locus_tag="IWL52_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1890..>4154)
                     /locus_tag="IWL52_002607"
     CDS             complement(1890..>4154)
                     /locus_tag="IWL52_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779113.1"
                     /translation="DKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNGAEIFQGTYP
                     ISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPIFNDIRKSVL
                     NKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKANFLEFFGGKE
                     GSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQSVLVGVYPFI
                     SSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNID
                     KQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNV
                     LTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMIT
                     GQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLD
                     GGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDY
                     VSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTS
                     GDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQV
                     YKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVRVDIDPMFAE
                     TVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRLGYEDIRLKE
                     DTAYDSTVLDQKRF"
BASE COUNT         1180 a          873 c          641 g         1460 t
ORIGIN      
        1 atatatagaa actgtttttc cttcacgata aagctttgct gatccggtat tcccgtttgt
       61 aactgttaat gtggtagtag ctgtatcatt aatgcgttca tcgatcttat tgtctaattc
      121 atctatagca gtcgcattag cattcgcttt tgtttgagca tccttagctg tggtgtctac
      181 ttcattaatt gaagcagtca actgcgaatt aatctccgat actttcccat cggtataatt
      241 gtttgcttta ccagtaattt cagaaatttt aatatctgtg gccaaattat cttcgacata
      301 ttctggtgct agatcccaaa cataatcttt tggattgttt gaatcacgca taccagtacc
      361 acgatattta tactcactaa tattcggggt tcgagtattg ccttcctcta gttttaacca
      421 cttaattgta cattgtccta cacttgtgct tggtacttga taaatctgca cttgtggggt
      481 agtagggtgt gagtcatctg ctgctgtaaa tgttttagac cacacattag ttaagccttc
      541 tactggttgt aagtcaccaa cctcccatgc atctcctgtt gctcgtgtga aaaatggtct
      601 aaaaacctgt gttgctggct tagttccttc aagtgtgatg gtatacttct taccttttac
      661 cattggttta atgttatagg tgttaataag atagttacta ttagtaactg gttgttgtga
      721 ttctggttta attagattct cacccaaagc caccttactc aaataatacg gtgcatcaag
      781 tagattaggc tggtacggtg tggctgttga gccttcttcg attttgatgt cgtacaactt
      841 aaatccgcca tttattttat ccctgtcaac aaagctaata ctcatataaa atcgatctaa
      901 atttgtgatt tgataattaa cgttagctgt acctttgatt gttatttctt tccctacatc
      961 atctgttgta atatttgtac tatttgcttc caataatatc ttttctcctg gtgatgtacg
     1021 atacaccaaa cgtaatttat caatagctcc tgtagttcct tcatcaaatc gaactttcgc
     1081 actcagagta taggttttcc cactagtaag ctgaggtgta ttaatacgcg taaacataat
     1141 aatgcttcct gtaccatcag aagtaaaatg taacttttcg ttatccaaag ttagtgaccc
     1201 gtggtaacca acgttaaaat cgctcgattt tagtttggac attaaattcg gcttaccgct
     1261 ataatcatat cccccgaagt cgatgctgtt actgtacatc acttgtaagt tacctaactt
     1321 agaaatttct tctttcagag catctaactt gtcttgtagc gttttagctt gaccagttaa
     1381 atcagtaatc tgttgattta agctatccac tctacctttg atttcagcca taaaagcatc
     1441 aaaagtttcg ttgtactttc gaatcaactc ttctaattgc gaaacatatt catcggcttg
     1501 gccttgcgaa atgtcagaca ctcctagtga gaaaaaaatg atatcttgcg ttgttaaaat
     1561 ttgattgtct tttctatatt ctacgtagca gtgtttataa tatcctgctt cactcataaa
     1621 tgtgccatca agagaaaacg tgacttcttc actagttaca ctagttgcaa cattatctac
     1681 gtaacggtta gatggtgttg ttccttttaa agtaaatgtt ccgccactcg tatccatctg
     1741 caagccattt agaaacggtt taaccgtcac cgtaatccct ttatcacctt gacgagccat
     1801 aatagctttg gtgtagttta attctttgct gaaatctaaa gccaaattat ataaactgct
     1861 agccatttat atacctcctt gtcttcgttt taaaaacgtt tttggtcaag cactgtgcta
     1921 tcatatgctg tatcctcttt taatctaata tcttcatacc ctagacggtg tgccactaaa
     1981 ttccatctaa ctaatacgtt tggcttacta gtttcaatga tgaaatggtc aatatcttca
     2041 tgagtaacag cacacaaaac tagttctgta ggtgtcacat gtgtcatata ccgacttaga
     2101 tttactgttt cagcaaacat ggggtcaata tcaacacgaa ctttaccatc gtcacctgta
     2161 acggcttccc cataatcagc gaaataatat tctggagttt cataagcgtt caatagtcgt
     2221 tgtccataat gttctgttgg tacagttgag tttttagtac ctctaacagt aaaatcttta
     2281 tatacttgta ccgttgattg ttcaaaccta gcaagtttcc catcttccca tgaaccaaaa
     2341 aaacaacctg gtaacgttag cataccatca ctagtaaatt tcatagtcct accagctacc
     2401 ttaaattccc atgagttacc cgcactacca ttaatgctta aagaactacc gtcgccagaa
     2461 gttacataac tagcattgct atacctgaaa ttgggcgcac caaaagatag aaacggtctg
     2521 ttattaccat tatcccacgt actaaaaacc aagttaccct gtggatttct aatcatgaaa
     2581 ccaccaccag ttttcatggt gtatgataca ataccggcat cagcacttac ataatcacgt
     2641 gcttctagct ccataatatc tttgttaact ttttttgagt accaagtcat tttgccatta
     2701 gcaatacttg ttctataatc agcaccatca ctaattaatg tagtacctct aatagtaatt
     2761 cctactattt caccagccgt aataaacgag gcattgaatc cgccatctaa cgtccatgcc
     2821 gtttcatatg ttccattaat gccagtttta gaaaaaccaa taccagcatt gttgatttgt
     2881 aaaacattcc ttgcggtatt cttatctggt gtgtccataa tcaaaatacg actaggcgct
     2941 tctttaggat ctaataaaac ataaccacca ttttgaccag taatcatatc agtttgatga
     3001 tctacaatat cattgagtaa atcactgatt tcgccaccgt ttttcaattg atcaatggca
     3061 tcattaatca aattgctgac attattctct gtgttttcta agaagtttgt tttgacgttt
     3121 cctacaacta atttatcgta tgaattggtt agaacattaa acgtatattc cacaattctc
     3181 gctgacatat tcacttttaa ctgtggatga tacacatcta ctccgtcacc catcgaaact
     3241 ttttctagat caacaaattt ttcatagcct ctttgatgcc tcaatggtac taattcaatc
     3301 gaaccactca cttgtggttt ttgtttatct atgtttgttt tcaaccagtc tttagcagct
     3361 tcccttaatg tggctacatc agtcgctttg tctttaaaat caacaaaaga aacatatcca
     3421 gcaggataat catccacgta atccgtgaaa ataacttctt ctggtagagt gatctcgtct
     3481 tctccttctg aagagctgct aatgaatgga taaactccaa ctaaaacact ttgagcatca
     3541 atctctaagt caagaccagt taagttttta gtataaatcg ctttgatttt atgatccgtg
     3601 cctagccttt tttcatgacg taatgtgtta ttatctttta gaaattcccc atgaaatcga
     3661 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
     3721 acattagcaa gcgtatctac taatgacgag aaagaaaact gtgaaggaat aactggtttc
     3781 gctaaagttt ttgcgttttg ccatgcctga ctagcagtga tcttttctgt tccactgtca
     3841 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
     3901 gtattgccta ttacagaagt ctttgcataa taaatccgta gacgctgttt tgctcgattt
     3961 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgctt
     4021 attggataag taccctgaaa tatctcggct ccgtttaatt tattgctaac agtagctgtt
     4081 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
     4141 gcgtcgtttt tatc
//
LOCUS       DACDVT010000135         4147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDVT010000135 DACDVT010000000
VERSION     DACDVT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.139"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            80..388
                     /locus_tag="IWL52_002608"
     CDS             80..388
                     /locus_tag="IWL52_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779114.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            392..754
                     /locus_tag="IWL52_002609"
     CDS             392..754
                     /locus_tag="IWL52_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779115.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            975..2381
                     /locus_tag="IWL52_002610"
     CDS             975..2381
                     /locus_tag="IWL52_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9779116.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            2828..3802
                     /locus_tag="IWL52_002611"
     CDS             2828..3802
                     /locus_tag="IWL52_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9779117.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEVGAE"
     gene            3799..>4147
                     /locus_tag="IWL52_002612"
     CDS             3799..>4147
                     /locus_tag="IWL52_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779118.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQ"
BASE COUNT         1445 a          602 c          928 g         1172 t
ORIGIN      
        1 aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat
       61 tctggaaatg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
      121 tgaattgatg tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag
      181 tgaaatgctt gaacgaatta tggatgttct caactcggtt atcaatgctg atttagcaaa
      241 atcttcagtt ttgactaatg aatttgttca taagcattta gcgcaaacaa ctgatgaatc
      301 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
      361 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
      421 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgc caacagaaaa aacgcttgat
      481 tgagaaagaa aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt ttgaaacttt
      541 tgctgatttt gagcggtggc tatcgtcgat ttctgacgat cagcaacacg aaaaatcagt
      601 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga
      661 atctgacggc ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
      721 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
      781 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
      841 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
      901 tgttactctt tgacaaagag taacagaaag gtttacactc tttttattag cgacagccga
      961 aggagggttt taacttgggg ttcagtattt cgttcaaaaa gggaacaaag aatacaagca
     1021 tttctcataa taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata
     1081 ttgatttcac aaaatcggat gaaaatatct atttagaaca aacaaatatt cgtgaaaaat
     1141 atgaagaatt attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata
     1201 gaaaaatcga ggactattta gcaaaggttc gcaaggataa aaaattggaa ccacagcgag
     1261 aatttatcgt gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt
     1321 ctactggcat atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag
     1381 ttgagtattt caaagaattt aaagaacgga atcctaattt atccgtctac aatgctgtta
     1441 ttcataatga cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt
     1501 ataaacgggg tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac
     1561 gagcagataa agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt
     1621 caattgaacg tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa
     1681 tcaaagatgt tcacgaatat aaagaaatca tgagcgagat ttcagaattg cgtcaaacgg
     1741 ccgtaaaaga gcgtgagagc gtttctgatg agttgaggta tatttctagg caaagagaag
     1801 aaaatgagcg agaaaaggct ctggtggctt ctgagagagt gaagattgaa gaagagaaga
     1861 aaaatatgct gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat attgttcctt
     1921 tagaaagttt caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg
     1981 atttgtatca acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg
     2041 agcaatattt acagatgctt gagcaaaata cagaattaaa tgagtatgta gctacattgg
     2101 aagaagagcg tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa
     2161 agctgaaaga agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc
     2221 aaaaatcgat cactcgtttg tctatccgtt ttggtcttgc agaaaaggat atggggcttt
     2281 ctgaggaatt agaagtgtta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac
     2341 agcgtaaatt agagcgttct gaagatgaga tcgagtggta gttacgatgt gcagtcaacg
     2401 gtaccggagg cggtttgtgc cgaggataag gagttgacgg ctaaaccact agcctattaa
     2461 gctgacattt gctaaaaaat ggccagtcca ttttttggcc aacctagcgt tagcgaagtt
     2521 tgggggcgcc cttttcaaag ggtgcggttt tattttttgg atatttgcat agctaaagag
     2581 aagtgctatt attgagttag caaacaaatg aactgaaaaa agagtaaaaa aatagtcgag
     2641 caacgctacc aacgttctcg acctttacgg aaactaatcg cactagctcc gtaacttctt
     2701 aatcattata cgaatatttt tagatttctt caagactaga gattttttgg taatgaattt
     2761 ttaagagtta cgtgaacttt tgaaaattct ttccagaagt ctctttttct ttgaggagga
     2821 acaaaatttg agtaaacgtg gcacgatcgt gaatgagtgg gaaaaactta accgcatttc
     2881 taacaggcgg ttaaagtatc aaagtttgac ccttccctta aaaatgcagt ggtcaattga
     2941 ccgaattacc atagtcggaa aactgaaaga aaatatttat tatcacactc caaatgatgt
     3001 gttgatttta aattttgaac agcttatgcg tttgaacgaa ggcaatggtt atttaaaagc
     3061 tgttgggaat aatgggtggc aattattaga ccaacacgag gaaaatatag cttatattga
     3121 gattcttaaa tttcatgaag gaaaagggcg gattgacttt aatcctaata aaatcaatca
     3181 atttttatca ggttccatga aaaatttcat tcatgattta tttctagaac ctcatttttc
     3241 acgtgcggat attgcttgcg atattgtaga tattcccgat gactttgtta gtcagtatcg
     3301 tgtagttgat cctgtgtctt ttaaaccaat ttatgggcgt tctggtaagt tagaaacggc
     3361 atattggggt agtcgtgcca gtgaacgaca aattcgaatg tacaataaaa aattggaaca
     3421 agaaagaaaa cgtaaaattg ttccgccgga aataacgaca tggtggcgac tagaattgca
     3481 gttacgaagg ggaaaagcaa ctgattggca tgagatggtt catgaaagtt tggatagctt
     3541 tgctagtcca cattattttc cgccaaatac taaagttact gaacggatta tggtgaaagg
     3601 tttgctttat gatcattctg agtggggaaa tctagctaag aatacaaaga aaaaatacag
     3661 ggatttgttg aagcaagaaa gtcaaaatga tgaattgacg aatcatttgc gagagtcatt
     3721 tgcggaatcg gcagacgatt tgaaacgaga attagataca tggctccaag gacttgatgt
     3781 aacggaagta ggtgctgaat gagtaatcca gtagaaaaaa tgaccataaa aaatcttgct
     3841 gacgaattgg gtgtatcgaa aaatacagtg cgcaatcaac tggtcaattc agggtttgac
     3901 cttgctcagt ataaagaaaa tggcattatc atgctagata aagagttgat tggtgttgtt
     3961 cggtcaattt atcggtcaaa aagtgatcaa ttgactggtc aattaccggt caataacaca
     4021 ggcagtgatt ttcctttaga aaaagagaat gagtttttgc gtgatcaatt agctgagaag
     4081 gacaaacata tttctgaatt aacagaaatg attcgtgatc aacaaaaatt gaccctgctt
     4141 cagcaac
//
LOCUS       DACDVT010000136         4134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDVT010000136 DACDVT010000000
VERSION     DACDVT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.140"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..552
                     /locus_tag="IWL52_002613"
     CDS             <1..552
                     /locus_tag="IWL52_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9779119.1"
                     /translation="NAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSH
                     PEMGHMLIKRHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEA
                     FYLAQCAYNTTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVT
                     PELGDSAGIIGGLALARKAIRNK"
     gene            complement(2043..2708)
                     /locus_tag="IWL52_002614"
     CDS             complement(2043..2708)
                     /locus_tag="IWL52_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9779120.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2834..>4134
                     /locus_tag="IWL52_002615"
     CDS             2834..>4134
                     /locus_tag="IWL52_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9779121.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQIT"
BASE COUNT         1304 a          647 c          779 g         1404 t
ORIGIN      
        1 aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca
       61 tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa
      121 gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt
      181 tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt
      241 gaaaaacgat tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt
      301 gaagcctttt accttgccca atgtgcatac aatacaacac tgatgctttc gccagataga
      361 attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa
      421 tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgctagcta cattgttacg
      481 cccgaattag gtgatagtgc tggaataatc ggcggattgg cattggctag aaaggcgata
      541 agaaataagt aattttaatg aataaatgtt tcattggtac tcatgtcaaa agtatttaac
      601 tgagtaatca aatgatgttt gataaagcga aaatgaattc aaataaaaaa ggatcgagca
      661 aactggaaca tttgtccgat cctttttcac attctattta tttcgtttat tcaaccaatc
      721 ggtagagaca tccattgcgc tcttgtcgcg tggctttgat ccatataatt gaccattacg
      781 aaatcatgga tcatcgcttt gaatgcgaac ttgtgtcaca tctacttttt tgaccagatt
      841 ttgaccagtc tattttacat tctaatgtac ttatatgagt taattttttg agtaaatggt
      901 aagtttttcc ttgtttgttt tatcttaaac tagtgtgtaa tttgaaaatc tatatggaag
      961 ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag gttcctagcc
     1021 tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt atcagctata
     1081 taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg tagctctgct
     1141 tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga agataagata
     1201 actgtgttat ataaactagg taactaactg taacaagcta attttaagag attcttatta
     1261 gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca tttcaataat
     1321 gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg gaaaaaagtt
     1381 cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca agagataaaa
     1441 cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa tcccatagat
     1501 taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa agtctcttcc
     1561 tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa caatccattt
     1621 tgtattttat tgagtactct tggataaact accagcgatt atcatattta tttatgttac
     1681 tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa gaactaaaat
     1741 tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact agattggtcg
     1801 ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct tgattatgat
     1861 tccaaaaaga atcattggaa ggctgtggcg taaatattat actataagcc agaaatacct
     1921 atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa ttaatttgta
     1981 cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg ttcatctcct
     2041 acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata gtcttggctg
     2101 aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac gttcatgacg
     2161 tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc tccagcgtga
     2221 aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc gatggaattg
     2281 tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat
     2341 cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac caaacaaaag
     2401 acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga tgaataagat
     2461 ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt agctgattca
     2521 atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag catttcaaag
     2581 tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac accaattttt
     2641 tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat tttttctccg
     2701 gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac atgacctata
     2761 cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag aatcatttaa
     2821 gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa tcatagtgac
     2881 cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct atcggacaaa
     2941 tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac attttattgg
     3001 gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag aaactgagct
     3061 ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt ggaacttcaa
     3121 gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat cttctcacga
     3181 gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat cgatcaaaat
     3241 aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct atgaacgggt
     3301 catttcacta ttcactgatc gattcaatca agcatttcac actgattatg ccactaatat
     3361 ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag acgtatataa
     3421 ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat catccttttt
     3481 cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat tacatcaagt
     3541 attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg caggaatcat
     3601 tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc agatgtacgt
     3661 tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag ttgaaaaaat
     3721 cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat ttggattgtt
     3781 gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt tttcttcttc
     3841 acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact atcatgtatt
     3901 ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc gttttgcatc
     3961 agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat tagtcaatgt
     4021 atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa taaatctgaa
     4081 ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa taac
//
LOCUS       DACDVT010000137         4027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDVT010000137 DACDVT010000000
VERSION     DACDVT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.141"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(225..938)
                     /locus_tag="IWL52_002616"
     CDS             complement(225..938)
                     /locus_tag="IWL52_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9779122.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(1192..1893)
                     /gene="nagB"
                     /locus_tag="IWL52_002617"
     CDS             complement(1192..1893)
                     /gene="nagB"
                     /locus_tag="IWL52_002617"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9779123.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            2127..3659
                     /locus_tag="IWL52_002618"
     CDS             2127..3659
                     /locus_tag="IWL52_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9779124.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(3850..>4027)
                     /locus_tag="IWL52_002619"
     CDS             complement(3850..>4027)
                     /locus_tag="IWL52_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9779125.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1293 a          832 c          694 g         1208 t
ORIGIN      
        1 acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg
       61 agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg
      121 catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg
      181 cttattactt gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg
      241 actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa
      301 gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg
      361 aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta
      421 agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt
      481 acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc
      541 gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa
      601 gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag
      661 aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca
      721 gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc
      781 aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga
      841 tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt
      901 aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata
      961 tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga
     1021 ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc
     1081 ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa
     1141 tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt
     1201 acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc
     1261 tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc
     1321 gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac
     1381 agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga
     1441 ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc
     1501 gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat
     1561 ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt
     1621 ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc
     1681 atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc
     1741 caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc
     1801 taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc
     1861 ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat
     1921 agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt
     1981 tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc
     2041 aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc
     2101 agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc
     2161 aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc
     2221 ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg
     2281 agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg
     2341 atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg
     2401 caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct
     2461 tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt
     2521 tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag
     2581 gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa
     2641 aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg
     2701 atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga
     2761 tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg
     2821 gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc
     2881 gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt
     2941 atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat
     3001 tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc
     3061 cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg
     3121 atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc
     3181 gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa
     3241 aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc
     3301 tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag
     3361 cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa
     3421 gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga
     3481 caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa
     3541 cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc
     3601 atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa
     3661 cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct
     3721 tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata
     3781 tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc
     3841 tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa
     3901 gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg
     3961 ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat
     4021 aaataca
//
LOCUS       DACDVT010000138         3771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDVT010000138 DACDVT010000000
VERSION     DACDVT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.142"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..182
                     /locus_tag="IWL52_002620"
     CDS             <1..182
                     /locus_tag="IWL52_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779126.1"
                     /translation="ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRN
                     SSYGSDYTFAITYTI"
     gene            345..791
                     /locus_tag="IWL52_002621"
     CDS             345..791
                     /locus_tag="IWL52_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779127.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            793..930
                     /locus_tag="IWL52_002622"
     CDS             793..930
                     /locus_tag="IWL52_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9779128.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            968..1261
                     /locus_tag="IWL52_002623"
     CDS             968..1261
                     /locus_tag="IWL52_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779129.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            1258..1482
                     /locus_tag="IWL52_002624"
     CDS             1258..1482
                     /locus_tag="IWL52_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9779130.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1479..2504
                     /locus_tag="IWL52_002625"
     CDS             1479..2504
                     /locus_tag="IWL52_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9779131.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            2693..2766
                     /locus_tag="IWL52_002626"
     tRNA            2693..2766
                     /locus_tag="IWL52_002626"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2727..2729,aa:Ile,seq:cat)
     gene            3443..3771
                     /locus_tag="IWL52_002627"
                     /pseudo
     CDS             3443..3771
                     /locus_tag="IWL52_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1307 a          639 c          752 g         1073 t
ORIGIN      
        1 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
       61 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
      121 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
      181 agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
      241 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
      301 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
      361 aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg
      421 ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa
      481 tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag
      541 atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg
      601 atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata
      661 ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt
      721 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
      781 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc
      841 actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa
      901 atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag
      961 ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt
     1021 ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac
     1081 tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc
     1141 agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc
     1201 agaaaaattg gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg
     1261 aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct
     1321 actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc
     1381 atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac
     1441 aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta
     1501 ctgcgatgag cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta
     1561 ttaatgacga gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca
     1621 aaattatttt gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga
     1681 aaaataatta taacggaagc aattcttata ctacagatgt tattggtgac ggtgggattg
     1741 tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg
     1801 cgcctgtaca gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc
     1861 gagcttatat tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg
     1921 atcaaggaac ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg
     1981 tttgggggaa ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa
     2041 agcttgctta tgatttagct catggattta ccgatgaaaa tccaacaact tctgaaaaca
     2101 agcctgtcat tgatccaaca cgagctggtg cagctaatcc tacgctgaca gatggaacaa
     2161 atcacgccca cattgatcag tttggggaaa tcgaaaacgc aaacttgcat gtggctggat
     2221 ggcacattgc taactataaa tacgagtata ttttcattat ggactacaat actgggaaag
     2281 aattagctcg agtaagagct gatggaattt atagaccaga tgtaaaccaa gcttataata
     2341 cttctggaaa tgttggttat catgtatctt tcaatatgcg taattttcct agtaagaaag
     2401 tctatgtaat gatgcgggca acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag
     2461 atttccatga caaacgttgg tatttaaata ttcctaaacg ataaaaatag ctcctcgttg
     2521 aggagcagta cataactata ttgacaacta taaaaattat tcgataaaat agtgatgtta
     2581 tcgcatatct tcactatcac ccataatagt cacactccaa gctatgcgat aacaggtttg
     2641 ttgccacaca ttctactggt tgattgttta tggctttatg tggcaacaac cagtaccctt
     2701 agctcagttg gtcagagcag acggctcata accgtccggt cgtaggttcg agtcctacag
     2761 ggtacattaa cgtagccatt tgaatcgttc tgtgttagaa tttttttgaa gagtattata
     2821 caagctaaag cttttcttca ttgccactca aatgagtggc ttttttatgt atccttttat
     2881 ggattaatga aaggatgttt cacatagtta tacttctgta tatttgaaaa gttttacttt
     2941 gatttttaaa tagaaagaca tttgggttaa attgtgagat aataataaag aagagtttaa
     3001 agcgcacccc aaaccacttc cccataagtg tgttacgctt taaactcttt tatatttgaa
     3061 gccattaaaa agcataccat atttttgaaa aaaagtgaga aaaaaggctt acaattggag
     3121 tggtagttaa ttagtgactt atttttgatt ttatagcact gatactataa aatatagata
     3181 tcatcatatt acacaatctt aatactaact taaaaaatat ctcctttcac aagtatggtg
     3241 ataaaattcg ttccgggcta cctttttagg tagcctactt taatctttat acctttctgg
     3301 atcaacgaaa gtatacttta tatagtcata acgccgatga tcgctacgtg cgtccggcac
     3361 gtcagtcatg aatcggctac actagactag acagaaaaaa taaggtgtgt agaataataa
     3421 aaaacacact ggaggatttt tcatgtcaag acgtcaacga agaacctatt caaaagaatt
     3481 caaacaacaa atcgtcgatc tctatctcgc tggtaagcct cgcgcagaaa ttattcgaga
     3541 gtatgagctt acgccttctt ctttcgataa atggatgaag caagcacaat caacgggctc
     3601 attcaaagaa agagacaact taacaccaga acaagcagaa ttgatcgcac taagaaagaa
     3661 aaataagcaa ctcgaaatgg agaatgatat tttaaagcaa gcggcgctga tattcggacg
     3721 aaaagacaag taattgatgc caacaagcat aaatattcca tatcagcgat g
//
LOCUS       DACDVT010000139         3398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDVT010000139 DACDVT010000000
VERSION     DACDVT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.143"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IWL52_002628"
     CDS             <1..276
                     /locus_tag="IWL52_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP9779132.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IWL52_002629"
     CDS             419..640
                     /locus_tag="IWL52_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9779133.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IWL52_002630"
     CDS             656..1498
                     /locus_tag="IWL52_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779134.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IWL52_002631"
     CDS             1561..3363
                     /locus_tag="IWL52_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP9779135.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          468 c          702 g          999 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcctatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DACDVT010000140         3370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDVT010000140 DACDVT010000000
VERSION     DACDVT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.144"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWL52_002632"
     CDS             179..775
                     /locus_tag="IWL52_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9779136.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWL52_002633"
     CDS             899..2698
                     /locus_tag="IWL52_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9779137.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWL52_002634"
     CDS             complement(2976..3188)
                     /locus_tag="IWL52_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779138.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          946 a          589 c          605 g         1230 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt
//
LOCUS       DACDVT010000141         3304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDVT010000141 DACDVT010000000
VERSION     DACDVT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.145"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..581)
                     /locus_tag="IWL52_002635"
     CDS             complement(<1..581)
                     /locus_tag="IWL52_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779139.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLG"
     gene            complement(591..1328)
                     /locus_tag="IWL52_002636"
     CDS             complement(591..1328)
                     /locus_tag="IWL52_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9779140.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(1379..>3304)
                     /locus_tag="IWL52_002637"
     CDS             complement(1379..>3304)
                     /locus_tag="IWL52_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9779141.1"
                     /translation="TIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQ
                     ELNGKIAELKDSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAA
                     FGILLPAILAVSYLFGPMMLIIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLG
                     DTWESIKDGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQ
                     ETWQTFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAG
                     QLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAI
                     STTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISST
                     ASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISS
                     KVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFD
                     NLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGP
                     EAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQ
                     IIDSITKIQNDNASAWR"
BASE COUNT         1008 a          709 c          507 g         1080 t
ORIGIN      
        1 cctagacttt tttcatgacg taatgtgtta ttatctttta gaaactcacc atgaaatcga
       61 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      121 acattagcaa gcgtatctac taatgacgag aaagaaaact gcgaagggat agctggtttc
      181 gctaaaactt ttgcgttttg ccatgcctga gtagcagtga tcttttctgt tccgctgtca
      241 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      301 gtattgccta ttacagaagt ttttgcataa taaatccgta gacgctgttt tgctcgattt
      361 tcatctacat aacactgaat aatacgtccg tctacaatca aatctgcatt agttccgttt
      421 attgaataag taccctgaaa tatctcggct ccgtttagtt tattgctaac agtagctgtt
      481 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
      541 gcgtcatttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
      601 ccgttacgat aaatttgtat tttgctcaaa ccagtgcaat taaaatgatt gatatccact
      661 tgcaatgtcg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
      721 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacatcagt tcctgctact
      781 aagccttcga aactaaacac ataatcattt aagatgaact ggcatgaagt agctgaagga
      841 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
      901 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtaata
      961 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     1021 tggataacgt aatagacgtt gggatcgtca gagaattcta atttctttgc atagtttaac
     1081 caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccac atcctctaaa
     1141 tagttatagt gcaatgtaaa cgacatatct tcaaacgagt aatcttgtac taagccacct
     1201 aacctaccta aaacagaagt ttcaactctc tgtctttttg gagaaggtat ggttggtctt
     1261 tcagctaaag ccaatttatg caaataatca ggaaatccat cgattataga atgtatacaa
     1321 tcagtcattt tttcacatcc tttttaatac taaaaaaaca ggagaaatac tctcctgttt
     1381 aacgccatgc cgaagcatta tcattttgaa tttttgtaat gctatcaatg atttgttgag
     1441 ttgtttgctt catagtaacc tcatctgcgt taccatcaat tgtgaaattg aattcgtagt
     1501 tattcacagg ttgaatcgtt tgtgccctag atgaaactga tgtgctactc aagatacgat
     1561 caccaatttc ttgcaaaaca gatcttttca aaggtaaaac tgcttcaggt cctgcttcac
     1621 cgacaccgtt cattccacct agtaaagttg gtttagtaaa gatacctcct ttagcatgcc
     1681 attttacacg caaatggggg atttgacctt ttagcgggtt aaagctgcct tccatgataa
     1741 attccggtaa cggaatatgt ggtatagaaa tattcaaatt atcaaaaatg ccactgattt
     1801 tgtctctaat ccaatcaatt ggagcgctaa cagtcttttt gatgccttcc catatgttag
     1861 caattgtact tttaacatta ttgaatatgt cggaaacaat acctgttaga ttggaccaac
     1921 cgcttgaaat tgcatttttt ccatcgttta ctttagagct aatagtgctt gtaattccat
     1981 tccaaagatt caaagcagta tttttgatac cgttccaaat tccgctaatc cacgaagata
     2041 tactattcca aacactttga atggcacttt tagctgcgtt tatagcattg cttatactac
     2101 tagtcacact attccagata tttgatgctg tagagctgat tgaattccaa attccaccta
     2161 accaactaga tacagttgac caaatatttt gaattactgt agcagctgct tgtaccaagc
     2221 tagtgattgt attcttgata ctgttccaaa tactagaagc tgttgcacta attgaattcc
     2281 aaatatttga agccgtagta ctaatagctg tccatatacc attccaccat gccactactg
     2341 gatcaaatat agtatggaat gtagttacaa tcccattcca agcgatactt atccattgtg
     2401 tcatagtatc ccaagtattt ttaaggaaat cagaaatagg tgtccaaaca gcttgccaag
     2461 ctgcgcccaa taactgtcca gctacatcaa aaatacccac gataatatta ataccagctt
     2521 gaatcaatga cgttattaat gtccatggta tttgaacaat tcctacaatg tctgcccaaa
     2581 taatcgacca tacttctttg actccgttcc aaatatttga aacccaatca acgaatgttt
     2641 gccaagtttc ttggactcct tgccagatgt tggaagctcc ttcaactaat ccgctccata
     2701 gctctccaaa ccaatcagaa actccttgcc aaatatcttg aacccaatct acaaatccag
     2761 accaggtttc tttaacccca tcccaaactg atgaggctcc gtcttttata ctttcccaag
     2821 tatcacccaa ccaatcagta aattttttcc ataaaccact aaaccagtca gtaattgcac
     2881 cccagttctt aattgccaca ataacgcctg ctataacagc tataattcct ccaattataa
     2941 gcatcatcgg accaaataaa tatgacacgg ctagtattgc cggtaacagt atgccaaatg
     3001 ctgctgtaag accacctata gcaacaatga aatcctgtac tggttgtgga agattattaa
     3061 acgcatcagc catctttcct agaaaatcaa ttactggttc gagtgcatct atgattgtgt
     3121 tgcctatagg agctaatgaa tcctttaatt cagctatttt cccgttcaac tcttgcaacg
     3181 gagtagtaga atcttcattc attttttgtg cagatccact aacatcatca aatgtatggt
     3241 taacatcagt taaagattgg acaactttca tcgcattatc ttcgccaagc gcagaccaaa
     3301 ttgt
//
LOCUS       DACDVT010000142         3286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDVT010000142 DACDVT010000000
VERSION     DACDVT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.146"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..684
                     /locus_tag="IWL52_002638"
     CDS             <1..684
                     /locus_tag="IWL52_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9779142.1"
                     /translation="VGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYD
                     EIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVG
                     VQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYI
                     HSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVL
                     RSIIDKSIE"
     gene            914..1144
                     /locus_tag="IWL52_002639"
     CDS             914..1144
                     /locus_tag="IWL52_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9779143.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLRYTVLIQKIPNHLVYKNNVED"
     gene            complement(1367..1465)
                     /locus_tag="IWL52_002640"
                     /pseudo
     CDS             complement(1367..1465)
                     /locus_tag="IWL52_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1730..2185)
                     /gene="tnpA"
                     /locus_tag="IWL52_002641"
     CDS             complement(1730..2185)
                     /gene="tnpA"
                     /locus_tag="IWL52_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9779144.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2428..3286
                     /locus_tag="IWL52_002642"
                     /pseudo
     CDS             2428..3286
                     /locus_tag="IWL52_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1078 a          573 c          575 g         1060 t
ORIGIN      
        1 gttggcgatg ctactattat tcgcttttgt caaaaaattg gtttttctgg tttttctgat
       61 ttaaaaattg aaattgcaaa ggaagacttt agtcaaaaaa aagaacatcc gactagtgta
      121 aagtactatg atgaaattgc taatagttta acagaagctc tctattccac tattaggctt
      181 ttagacgaag aaaaattgaa tgaagccatt aaaatgatca ctcaatcaaa aaacatatat
      241 atatttggag tgggatctag tggaaatact agtcttgatt tagaaaatat gtttcttcgc
      301 gtaggagttc aagcaaaagc tgtactagac cctcattttc aagcccaagt cgcttcttta
      361 ttaacagtta atgatttagt aatcattttt tctttatctg ggaaaacgaa agatacttat
      421 gattcattga aaatagctaa aaaaaatggc gcaaaaatta ttgctataac taactatatt
      481 cattcaccta taggaaagtc tgcagactta gttttacaaa cagccattga agaatttctt
      541 aatggtggat ctttagctgg aaaaatatca caactttata tttgtgatct attagttcat
      601 ggttatgaac aaaacaataa tattgattca gttgaattaa gagaaaaagt tttgcgctct
      661 attattgata aaagtattga gtgaactacc cccacttagc taaacctaac ggtttttaac
      721 gattgaagta ggcaattctt gggatgagag catacttgat agcttatttt ttactaatag
      781 cggtggctct tattgaccaa atatccctgt agttcctacg gttcttgatt ttatctaaag
      841 atatttttat cgtggaagct attgatcaac ttgaaagaaa aacgtgtagc tcattatcac
      901 tagtttatcg agtatggatg aagcaatgga tcatcctctc ttaaataaat ttattaagga
      961 tctgatcctt caaatttttt ccatgatcga tgagcaggaa cctactgaat ctaaacgaca
     1021 tcgggcacaa ggaattatta agattgctaa gcgaatgttt tctataaagg gtgttctaag
     1081 atatacagtg ttaattcaaa agattcccaa tcatcttgtt tataagaaca acgttgaaga
     1141 ttaaaaaagt gtttcaatgc taaaattgca aaatacttca atgttatgag acaaactgtc
     1201 tataagaatc aatttccacc catttcatca cttgttaaaa gtttatgatc cttttgattt
     1261 ttatattaat ttatctgtat ttaatacaga ttttatttat tagttcatct gcctattgta
     1321 ttttattttg ttgccttacc atctgaagta aatatactct taattgtcaa attaagttag
     1381 acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga taatccagtg
     1441 attttctagg aagattattt ctgcgccagc attttctaat gtttgaattt gtgttttgta
     1501 atcttggttg cttgtgctaa ttcttgaata gccgtaaatt gccaaaattt catcaccctt
     1561 tctggacata agtttctgac accagttaaa atcaagtcta tcagtaaccc atacacccgt
     1621 gaggacgggt gtatttttaa tccccctaga aggggtcgac actcgctgcc ccttaaaagg
     1681 ggatttttaa aagaccgcca actgctcatc ttcactcact tacttctctt tattttttaa
     1741 atggttctac gtactctctt ttacttataa tatatcgaat gcgatcatca gcttcttgtt
     1801 cacggatgta tttttcattt gtcttttgat tcaatccgac tgtactcaca tagtatctct
     1861 tcgaccaaaa ggatttgttt ccgtatttat atttgagatt tgcgtgctgc tcgtggatta
     1921 gtaccgcact acgacctttc agatacccca taaaactgga tatacatatt tttggtggta
     1981 ctcgaacaag catgtgaata tgatctagca ttgcatgtgc ttctatgatt tccacatctt
     2041 tcatttcgca caacttccta agtatttttc caagatcttt tcgtaattgc ccatagatta
     2101 cttttcgttg atattttggt gtaaaaataa tatgatactt acaattccaa cgagtatatg
     2161 ctaaactttt atcgtcattt gacatgaaaa aactcctttc gatctgatga gcggttggta
     2221 gccactcact agtatatcga aaggagtttt tatcgctaaa gatacttctt ccacgcgtag
     2281 aaacgcgcgg ttttcttgtt tcgcacgctt tacgcacaaa actgactgaa gtcattaaga
     2341 aaataaaagg gtctaaaact tttaagtttt gtaccgaccc ccaaaagtta gaccaaaaaa
     2401 tctaactttt ggaggtcggt attttttatg gctaagtata cttttgaact taaattaaag
     2461 atcgttcatg attacttgga tggtaaaggc ggaagtgatt atctggcaaa gaaatattcc
     2521 attaaggctc catcacaagt gaagcgctgg atcaatgcct atcaagaatt tggtgaagag
     2581 ggtctagtaa gaaagcgaca aaagaaaaaa tattctgttc aatttaagct ggatgcgata
     2641 gagttatatc taacaagtga gctgtcctat cgtgaagttg ccaacacact taacatgaac
     2701 aaccctaatt tgatcgctag ttggatgcgt caattccgtg agggtgggat cgatggactc
     2761 tcaaaaacga aaggatgtcc acctatcttg tctaagaaaa atgaacccaa aaataagaaa
     2821 aaatctacaa ctaaagcaac gtctaaagaa cgtgaacgca tcgaggagtt ggagaaacga
     2881 gtccgttccc ttcaaattga aaatgccttt ttaaaagaat tgaggaagct gcgaaaacag
     2941 gaagcccaac aacgacgaat gaagcaatcg cacgaatcat cgcaagcctc cgaggaccgt
     3001 ttaaattagt tgagcttcta gaaactttaa agttccctaa ggcaatcttt atgtactggc
     3061 aaaaacgatt ggaccgtaaa aatccagatc aagagattga agcggaaatg cttaaagttc
     3121 gggagaagca caaggattat ggctgtttac ggatgaccaa tgaactaaga aaccgcggtt
     3181 tctctatcaa taaaaagaaa gttcagcgcc tgatcaagaa gctaggaata aaagtaacga
     3241 catacacacg taaatcacgg cgctacaatt cttatcgagg acaagt
//
LOCUS       DACDVT010000143         3269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDVT010000143 DACDVT010000000
VERSION     DACDVT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.147"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(560..1600)
                     /locus_tag="IWL52_002643"
     CDS             complement(560..1600)
                     /locus_tag="IWL52_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9779145.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IWL52_002644"
     CDS             complement(1949..2275)
                     /locus_tag="IWL52_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779146.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IWL52_002645"
     CDS             complement(2262..3050)
                     /locus_tag="IWL52_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9779147.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          985 a          547 c          455 g         1282 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttg
//
LOCUS       DACDVT010000144         3200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDVT010000144 DACDVT010000000
VERSION     DACDVT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.148"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..746
                     /locus_tag="IWL52_002646"
     CDS             <1..746
                     /locus_tag="IWL52_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9779148.1"
                     /translation="HSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMS
                     TNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSG
                     LSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGII
                     AAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLI
                     RYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            759..1766
                     /locus_tag="IWL52_002647"
     CDS             759..1766
                     /locus_tag="IWL52_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9779149.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1778..2782
                     /gene="neuB"
                     /locus_tag="IWL52_002648"
     CDS             1778..2782
                     /gene="neuB"
                     /locus_tag="IWL52_002648"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9779150.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2779..>3200
                     /locus_tag="IWL52_002649"
     CDS             2779..>3200
                     /locus_tag="IWL52_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9779151.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAIT"
BASE COUNT         1149 a          404 c          604 g         1043 t
ORIGIN      
        1 cacattcctt agcaaatata gttttagcac aattcgatag aattatgatt aattcttatt
       61 taggtcctgc tgcgggggga atatattcct acattagtaa cataggtatt attctaagcg
      121 taatatggat gtctactaat aacgcctggg ttccatggtt ctatggtgag atgagtaaaa
      181 ataatgaaaa aaaagtgaag aaagttagta actattattt agtagtcttt actttgatta
      241 cgatgattgt aatggtagtt tcgattgatt tagcaaaatt tatggctccg aaagactatt
      301 attctgggtt atctttagtg gttccgataa ccttaggcta tttctttcag tttttatata
      361 gtttaccagt taatgctgaa ttttatgaga aaaaaacatc gtttattgcg attggtacag
      421 tagcaagtgc tatagttaat atactattaa acatgatttt catacctagg ttaggaataa
      481 ttgctgctgg atacacaacc gtatttacgt attttttact ttttattttt cattataata
      541 tcgctaaaaa aattacgaat aaacaattat ttgacacaaa aaaaatatgt atcataataa
      601 tttttgtatc aattttttct ttgatacttc atattttaat caatcaaatg ttgattcgtt
      661 acgttttgac atcattattg ataattagtt tagttatata ttttttaaag attaagaatc
      721 gaattataga tgactataat tcataggagg agatataagt gtttaaagga aaaacaatat
      781 tagtaactgg cggaactggt tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg
      841 aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa acaattttat atgcaacaag
      901 agctctcaaa tcacactaat ttgagatttt taattggtga tatcagagac tctcaaagga
      961 ttgattatgc atgtcgtgat gtggatatta tttttcatgc tgccgcaatg aaacacgttc
     1021 cagcaagtga atataatcct atggaagctg taaaaacaaa tgtgatggga actcaaaatg
     1081 taatagatgc agcgatcaaa aataatgtaa aacattttgt aggtatcagt acagataaag
     1141 tagtgagtcc tactaacacc atgggtgcga caaagttatt gagtgaaaaa ttaataatag
     1201 ctgccaatca gtataaagga aaaacagaaa caatattttc ttgtgtaagg tttggaaatg
     1261 tcatggggtc aagtggatct gttattcctc tttttagtaa acaaataaaa aacaataaag
     1321 atattacttt gacagatgtt gatatgacac gatttatgat gtcaataccg gatgctgcaa
     1381 gattagttct gtctgctgct atgaattcaa aatttggaga aacatatgtc ttaaaaatgc
     1441 cggcaatcaa tattttaact ttagctcaat cattaataaa aatatataat gatacacatg
     1501 atgaaaaata tagaggtgaa ataaaagttg taggtatcag gccaggagaa aaattatatg
     1561 aagagctaat gactacagac gaaattgaac gctcatatga aaatgataaa atgtatatca
     1621 ttccaccaca ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa attaaaatca
     1681 ctgcatactc ttcagatgca acagaattaa tgagtggaca ggaatttatt agaatcgcaa
     1741 aagagaataa tgtcgtgact tggtaaggag ttttatgatg aaatcagaag tatatgtaat
     1801 tgcagaaatt ggtgtaaacc ataatggaag tctagattta gctttagaaa gtattgataa
     1861 agctaaagaa tgtggagcta acgcggttaa atttcaaaca tttaaaacga ataagctagt
     1921 ttccaaaaag gctccaatgg caaaatatca gaaggataat cttaaagagg acagttctca
     1981 atttgaaatg ctaaaaaaat tagaattatc tcaatctgat tttattagaa taaaagatta
     2041 ttgtatacaa aataaaattg attttttgtc tactcctttt gatgaagaaa gtgcagactt
     2101 tctaaaacaa attggtgtac aaggatttaa aattggctca ggagatttaa caaatttacc
     2161 ttttcttaga aaaatagata tgtatgggtt gccagtttta ttatctacag gtatggctga
     2221 tattcatgag gtcagtgagg ctgtagattg tttcatagat agtccagtta ctattttaca
     2281 ttgtacttca aattatccgg ctagttctga agatattaat ttattggcga ttaacacatt
     2341 aaaagaaatg ttcgggatac ccgttgggta ctccgatcat tcattgggat atgatgttgg
     2401 tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa cattttacct tggataagga
     2461 actacctggt ccagatcata aagcttcttt agatcctcag gaatttgcag attttgtaaa
     2521 gcatattaga aacactgaaa tatttttagg tgatggtgtt aagcgagcaa tgccttctga
     2581 acttagtaca aaagaagtat caagaaaaag cctagtggtc actgagaatt taaatgtggg
     2641 agatattctt acagagaaat ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa
     2701 gtttttatat caatatattg gcaaaaaagt taaaagacca ataagtgcag atagtgttct
     2761 gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa gtggaacacg agctgattat
     2821 ggaatatata aacctgttgc tgagaattta ctacaagata attgggatgt atcatttgtt
     2881 gtttctggaa tgcatttatc ccataaatat ggatttacta aagaattgat cttagctgac
     2941 ggatttagaa ttagtggaga agttcaaagt ttgtttttag agaacaataa gggaaacatg
     3001 gctcggtctg tggcattaga gataaatggt ttcacaaata tatttgaaca aactagccct
     3061 gattttgtat tattactagg ggatagagga gaaatgttgg cagctgcgat agcttgtttg
     3121 tatcttggaa tcaaaacagc acatattcac ggaggcgaag tgtctggtac tgtagatgaa
     3181 tcaataagac atgccataac
//
LOCUS       DACDVT010000145         3127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDVT010000145 DACDVT010000000
VERSION     DACDVT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.149"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            52..297
                     /locus_tag="IWL52_002650"
     CDS             52..297
                     /locus_tag="IWL52_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779152.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(481..1230)
                     /locus_tag="IWL52_002651"
     CDS             complement(481..1230)
                     /locus_tag="IWL52_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9779153.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1416..2120
                     /locus_tag="IWL52_002652"
     CDS             1416..2120
                     /locus_tag="IWL52_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9779154.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2160..2717
                     /locus_tag="IWL52_002653"
     CDS             2160..2717
                     /locus_tag="IWL52_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9779155.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2725..>3127
                     /locus_tag="IWL52_002654"
     CDS             2725..>3127
                     /locus_tag="IWL52_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9779156.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEI"
BASE COUNT         1058 a          451 c          621 g          997 t
ORIGIN      
        1 tttataaaaa aggaaatgaa taactgaaaa aattattata ggagagaaaa aatgtcaaaa
       61 tctaaaaaaa atatcaccat aattggttta ggcttaaccg tatatttatt tacagaattt
      121 gtttatagac atgtgaattt atttgcagaa cctttatttg tattatacga atcaaattcc
      181 tcaatcgtca gtgaacttgc taatttaatg atagcattgc tgtttactcc cgcgttgaga
      241 gtcataacta ttttggtctg tttagtgcta ttgttttttg ttaataaaaa gaaataacgt
      301 agtatttcaa aagacaaaga agtgggatga tgattgctat agtatgttat acgtggtgct
      361 aggtaagtga gaataaacaa aaaaggaaac acgcaataaa gtttatttca aaactttatt
      421 gcgtgtttct tttttagatt ttttatatct gttgctttag tagttaccaa tagttttcgc
      481 ttaatcaatc aaaacgattt tttgtttatt gtattcttta cgaacctttt caggaatttc
      541 ttctgaaata agataggtaa ggtcttcaat tggaccgacg atatgattac tgatggtgcc
      601 gaatttatca ttagttatca tcccaagtac ttcagtagaa atacgtatca tctcacgttt
      661 aatgtcggat tcttgcatag ttggcacgct cgttccaaca tctagatcta tgttataaat
      721 acccataata taaaggtcgg ctcgaatgtt ttgtagttgc ttataagctt gtaccccaat
      781 attgatcata gattgtttat aaaaagtacc gccaatattg ataatttcca aattaggatg
      841 ctgtgctaat gctatggcaa tcggcgggct attggtaatg attgttgcac gaaaatcttg
      901 tgggatttct tttgctaaag ccaagttagt tgtacttcca tcaatgatga tgacagattc
      961 ttcttttaaa aaagggaggg ctttatgagc taatgcagtt ttttgttcgc tagctacagt
     1021 ttctcggtag ttaaaattgg taattggcgg accaacacgt aaagcgccgc tgtgtactct
     1081 ttttaagaga ccttggctat ccaaatcttt caaatctcgt cgaatcgtgt cttcggatac
     1141 attaaaacgt tgacttaaat cactagcgat aactttttgt tctgcgttta ataaatctag
     1201 aattttttga tgacgttcta atttcaacat gttgcgtgct ccttcttatt tattcttgtt
     1261 tatcatgtca tttatgcgtt tttatgtcaa taatttccgt ttttaataca ctaaaaaggc
     1321 ataaacaagc aaaaacaaaa tatttttatg cagttttatg ttgactagta tttgttaagc
     1381 ggttacaatg agggggtaat taaaggaggg aatgtatgga gaaaaaaatt attttgtcgc
     1441 cttcacttat gtgtggggat ctagcaaatt tggaaggaag catccaagaa ctagaaagta
     1501 ttgggatgga ttccttgcat attgacgtga ttgacggttc ttttagtccc agtatgcctt
     1561 tagggatcga aacaatcaaa agaattagag aaatcacttc aatgaatttt gatgtacaca
     1621 tcatggctaa ggataacgat aacgaatttt ttattggaga aatgttgaaa attggcgtag
     1681 agagcattac ttttcattat gaaacaagcc tccatattga tcgatatatc cagctcatca
     1741 agaattctgg aacaaaagta gggatagctt tgaatccagc aactccgtta aatgtattac
     1801 aatatatttt acccgagatt gataggattt gtttgatgct gattaatcca ggttttgcaa
     1861 caaacaagtc agaaaaacaa gtttcttacg cgctaaaaaa aattacagat ctttgccagt
     1921 tagtaaatga gaaagagtta gcagtagata tccaagtgga tggacgggta tcgttagaaa
     1981 cgatcccggg attattagat gcaggcgcaa caaatctggt attaggaagt acaagtttgt
     2041 ttcttaaagg aaatacatta gcagaaaatc aaaaactact ttatcaagcg atgaagcaat
     2101 cagttaggaa aaatcaatag atgtagtggt cctgaataat agaaatggag gggaaatagt
     2161 tgagttataa ggcggcgaaa caaaatattg taagagaaat tactgaaaca ttagaccgta
     2221 tttcttcaaa agatgtggaa caattgataa agaaaattga aaaagcggaa cgagtttttt
     2281 tcgtgggggt tggcagagta ctgttagcgc tagaaggaat ggcaaaacgc ttagggcatc
     2341 taggaattca gacagtagtc gtaggacaaa tcactgaacc ggcaattaca gataaagatt
     2401 tattgattgt tggttctggt agtggggaaa gtgctttccc actaactatt gcaaaaaaag
     2461 caaaggaatt tgggtcccaa gttgcccata taggatcaaa cccagacagc acaatgaaag
     2521 aatattcaga tctttttatt cgtatacccg tacaaacaaa attagcgtta ccagaggaaa
     2581 tttcttctat acagccaatg actagcctgt ttgaacaaag tgttcttttg ttaggagata
     2641 gtattgcttt agtgatgata gaagaaaaag caattgatat gtcaaagttg tggcaatacc
     2701 atgctaactt agagtaggtg aaaaatgaaa gcggtacatt ttggagcagg gaacatcggc
     2761 agagggctta ttggacaaat tctacatcag aatggattcg atctatgttt tattgatacg
     2821 aatcctgaac tgatccatca attaaatcaa gataagggtt atactatcga aattattgat
     2881 gaaatgaaca ggagtgattt cattgatcat gttgtggcac tgaatagttt atccgagcta
     2941 gaagaggtga ttacagcaac aaaaaatgca gatatcttaa caacatctgt tggcgcaata
     3001 aatttagtaa aaattgcacc gatcattgcg caagccttaa gtaagcgatt tgaacaaaag
     3061 catagcatca atatcgtagc taatgaaaat gtgataaatg caagtgatgt gttgaaaaaa
     3121 gaaattt
//
LOCUS       DACDVT010000146         3010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDVT010000146 DACDVT010000000
VERSION     DACDVT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.150"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            30..707
                     /locus_tag="IWL52_002655"
     CDS             30..707
                     /locus_tag="IWL52_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9779157.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(844..1260)
                     /locus_tag="IWL52_002656"
     CDS             complement(844..1260)
                     /locus_tag="IWL52_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779158.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1263..1547)
                     /locus_tag="IWL52_002657"
     CDS             complement(1263..1547)
                     /locus_tag="IWL52_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779159.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2247..2342)
                     /locus_tag="IWL52_002658"
     CDS             complement(2247..2342)
                     /locus_tag="IWL52_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9779160.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          957 a          479 c          567 g         1007 t
ORIGIN      
        1 cggaattaac agtcaaatat caaaatgaat tgaatttagt accgctgaaa aatttcaatg
       61 ctaagcagat ggatttattt tttgcattat gcgctcgtat gaaagatcgt ggtttgaaaa
      121 aagttagatt ttctttcgag gaattaaaag agttaagtga ttataagatg actgctacaa
      181 aagcctttgt cggtgattta gaaaagctct ataaagatat gcttaatctt acttaccgta
      241 ctgaagatga ggattctatt cgctactttg ttttgtttac tggttttgaa atcaataaaa
      301 caaaaaagtt tgttgaagtc agtgttaatc cggacctaga atatattatt aatggtttaa
      361 cagctgaatt tagtagattt gaattatctg ctttcacgtc gttgcgttct acgtatgcaa
      421 aaactctatt ccgactgctt atgcagtacc gttctactgg ttattatgta gttcgtattg
      481 aagagtttag gagcttaatg gatatcccag aatactatca aatgggtaac attgatcaaa
      541 aagtattaaa tcctgcatta cgagaactaa aaaaatactt caatgatctt aaagtcacaa
      601 aaataaaagc aaaaaagggc aataaaatcg ccaaattaga atttactttt tcaagcctaa
      661 cgtcagattt gccaaagatt tctctgcgta actggttaga agaataaata aaaaagaact
      721 cgcccccctt acattaagca agtagacaag tcccttacaa ccgactaaga ttgctagtac
      781 attctagcat attgtatggg gcttctaaat aaaaaacaga taggaataac tcctatctgt
      841 tttttatcgt tttagaaaat ttgttgggtc gaaatttcta gaagtgactg tttgactggt
      901 ttctttttga tgattctttg gtcgatttcg atttgatgga tttagtttag cttcgatttt
      961 ttctttcact tcttgatata ctttagaatt tgtgccaata agaacgcctg tatcttttag
     1021 atataaatca gcaaattctg cgatgatttc agtcatgatg gaaatttctt ttcccatagc
     1081 atttagtttt gcctgattgc tggtagtggt cgtgttggta tcgggttcat aactcataca
     1141 taaaaagcga atggcttccg agtagttatt gatatctgga tttttttcga tgatggaatc
     1201 taaaacgtca acatgactag ggtacaaagt aacatgtttt tggacagaag tttttctcat
     1261 ggttatatca ttcctccgat aagcatttca atgatttgat tatttgtgta gacatactcg
     1321 cttaatattt gagtattttc tgtttggata gcaataaatt tatcgagcaa taaatcgaat
     1381 tggtcttttt tgtaagtcgt aagttgacgg tcaaaatcac tttcgattaa aacttttaga
     1441 tatttatttc gactccattt ttctcctgtt ttattcgtga gttcttggca acgtttgtct
     1501 attgttgcaa caaactttgg atcaatattc cgaactaaaa tgtccataaa aaatctcctt
     1561 ttgcaattat gtataattta tgtataccat acaaatacta tatttgtata gctgtttttt
     1621 gccaaaatgc tatacataag ctacccacga tggcgaaaaa gtgatatcaa atttttttct
     1681 gagaaacgtg ataggcacag cctatctacc cacttttttt agaaagtggg tacacgtttc
     1741 cttatgctct ttaaaattcg ggggggagtg ctgcacgtgt aatcgataac caaatttaac
     1801 agacgaaaaa ttgttggaat ataaaaatat gacacccgaa agaaaacgat cgttggaaaa
     1861 attaatgcaa ctagtgaacg aagacggcgg aatttcagac gcagcttttg acgcatggag
     1921 cgaatttcat ggagaatata attaagtggg tttaaatggc tctaaaatcg cttctaagag
     1981 ttttttagct tggaccaatg aaatacacca aaaactaaat aaaacgtctt aaatcgaaaa
     2041 atacgagctc tcagagcgac tttatttgta aatgttgcat ataagaaata attacggatt
     2101 ttagactttt gtaggatatt tttgaaataa atatttattt ctgttataat ttgattgcaa
     2161 ataagtgaac acgaaacaca cgaaatcccc agagaggtcg gattctctgg ggcttttttt
     2221 gtgggctgag tcgccttaat taagttttac ttcttgtttc ggttattcaa ccagtaactg
     2281 aacaaagcgc ttaaaagccc aacaagaagg gagcaaataa agttagtgaa cacgaaacac
     2341 acctccttcc agtcgcaaga ctgtcggata ggcgacaatc aattatacaa taaaaaagca
     2401 aaaataagcc taagtttttt attgccttgg ggttgttagg gcttgccctg accgcctgta
     2461 aggcgcttta gaacgaagga catagttttt atcgaatctt gtcttagaac ggcttagacg
     2521 agctctcagg acgccacaaa ggagtcccta atgaattatt tttggcgaag gagcctaaag
     2581 gcgactgctg caagtaaaat gtgagagcgt agcgatcact ccttcttaca gcgaccatag
     2641 ggagctagat tggcagttta atatcatgcg cgaacctgtt tattttaaaa agtgggtgat
     2701 tttttggcgg gaaattttgc tcgcgcgcgc gcgtttattt tatttatttt ttaaagattt
     2761 tttagaaccc tttttagggg gcagttgagc cttactctcc caacggatac agaagggtac
     2821 taacaacttt ttatctgagt actaacaact ttttatctga gtactaacaa ctttttatct
     2881 gagtactaac aactttttat ctgagtacta atatcctttt gtataataac aaccaaccta
     2941 tttacgagtt ggttgttaaa ggttattatt tatttgtaag gggggaagaa aggaatggat
     3001 gaaaaaacgg
//
LOCUS       DACDVT010000147         2952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDVT010000147 DACDVT010000000
VERSION     DACDVT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.151"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..860
                     /locus_tag="IWL52_002659"
     CDS             <1..860
                     /locus_tag="IWL52_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9779161.1"
                     /translation="NLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVS
                     AAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWR
                     EYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVL
                     LSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAK
                     QSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRN
                     PKELLIDNN"
     gene            complement(1146..2768)
                     /locus_tag="IWL52_002660"
     CDS             complement(1146..2768)
                     /locus_tag="IWL52_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP9779162.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRQFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT          949 a          502 c          546 g          955 t
ORIGIN      
        1 ataaccttaa agttggagat aagataaaaa taaaatctaa tttatttgat gctgataatg
       61 agaaaggtgc agatgaaaca gtagaagtag aaattaaagg tctattcgat gggcataata
      121 gcggtgtagt aagtgcagca caagaactat acgaaaatac attaatcact gatattcata
      181 cagctgctaa agtttatggt aataccgaag atacagctgt ataccaagat gcaacattct
      241 ttgtaaaagg tgataagaat cttgatagtg taataaaaga tcttgggaaa ttagatataa
      301 attggagaga atataatttg attaaaagtt catcaaatta ccctgcatta caacaatcta
      361 tatcaggtat ttactcaatt tcaaataaat tattttttgg ctcattaata tttgcaggag
      421 ttgtagtttc attattatta ttcttatgga tgaatgctag aaagaaagaa atagcagtat
      481 tactatcatt aggtatatca aaattagaaa tttttggtca attcctaatt gagatggtat
      541 ttatatcaat ccccgcattc gttggttcct atttcttagc tcaatataca gctgacaagc
      601 ttgggaataa tatattgaat aaggttactg gtgatatagc taaacaaata gctaaacaat
      661 ctgcatctag ccaattaggt ggtggggcag aagctgaagg atttaacaag acattatcaa
      721 gtttagatat caatgtatta cctaaattca tgatttatgt agttatattt atgagtttag
      781 tacttcttgt atcattgatt atttcttcat tcaatatatt aagaaggaat ccaaaagaat
      841 tattaattga caataattaa aattttcggt gctttttagc accaaaaatg agagtgtaat
      901 ataaaccgtg taagtaacca tcaatcctag gcgggtggtt gcttcacggt ttttattttt
      961 ctaaaataag atcaagaggt gattacacga tggctagaaa gaaaggaaat cctgatgctg
     1021 agaagttagc tgaatccatt ttgaatactt atcaacccga atctgtagag gatatgcaag
     1081 atgttttaaa agatgtgttt ggtcccttat ttgaaaaaat gcttcaagga gaattgaata
     1141 accatttagg gtatgacgcc cattctaaag agccaaagaa acatgacaat cgacgaaacg
     1201 gttatggcaa taagacgctt aaaacgagct ttggtaaagt aaacattgat gttcccagag
     1261 accgggaggc ttcttttgaa cctgagttga ttcctaatag aaaacgagat gtctccgaca
     1321 tcgcagactg tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta
     1381 tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt ccatagccag
     1441 gtggctaatt tctttaaatt cattgcagca aacactagca tcgtatgcgt cgcgactttt
     1501 tccaagcctc gatacttggt ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt
     1561 cgttcaatcg tttgtttccg ttttttgtaa attgatttat tcagatctgt taggcgaaga
     1621 tgttccactt catccatcag atctccccaa acatgtctcg tgatgactcg ttgatgattt
     1681 ttacttttgg tacattggtt tagcagagga cagttcacac attcttttgg attggatttg
     1741 tattctcggt acctttctcg agtaaccgtt gaaaaagcga gcatttgatc ttgcggacaa
     1801 atataacaat caaaatactc gtcatagaga aactcatttt ttgataatag cccctttttc
     1861 cctttgggcc ttgaataagg caaacaaggt cgaaggttca aatgagtcaa aaagtgaaca
     1921 aacttaggtg tcttataccc agcatcagcg acaacacaat tgatttcagg aaaaagtctc
     1981 ttcgattgtt tgacgagctg aaaggcgact tgactgtcat gaatatttcc aggagtgatg
     2041 attgtgctaa gaataaatcc attgtcgtcg catgaagtat gggcagaata agcaaattgt
     2101 ttttctcgtt cgcctttgac gtagtagccg ctttccggat cagctttact tatttttctt
     2161 tctttcagct tcacttcctt ttctaagtcc aaagtaaaag ggcgttttcc agcctctatt
     2221 cgttgacgat tgatctcatc ttctaattca tcctgatagg ctttcgcttc atcatgggtc
     2281 atttctttcg taaacttatg tttattggcg ttagctttga tgtgggtaga atccagatac
     2341 aaattatcct ctgttacaaa acctgcttta actgcttgtt ctaaaatata ggcaaaaata
     2401 tcctcaaaca ccgtcgtttc tctaaattgg cgaacataat tctttccaaa agttgagaaa
     2461 tgggggattt tctcctcgaa ggagtagcct aaaaaccagc ggtaagcgac attggtatcg
     2521 acttccttaa tggtttgtcg catggaacgg ataccgaata aatactgaat aaacaccaat
     2581 ttgatcagaa tcactggatc gatacttggg cgacctaaat cggagtaagt ggcttcgacg
     2641 attggataaa taaattcaaa atcaagcgcg gcatcaatct ttcgaacgag atgatcttga
     2701 gaaactagat catcaatcgt aagcatcgca aattgatttc taccattcgt attgttttta
     2761 gacatcatag ctatcactcc ttgcattcgt tttcataaat ctattataaa agaaaagact
     2821 gtagacatca tccataaaat ggactttgtc tacagtctgg ataacgtgat tttttgctgt
     2881 aatgacaagg ttttagctgt ttcagatacc atgtatccaa aaatagttta ttgagaaaat
     2941 aggagagtat tt
//
LOCUS       DACDVT010000148         2929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDVT010000148 DACDVT010000000
VERSION     DACDVT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.152"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            697..1029
                     /locus_tag="IWL52_002661"
     CDS             697..1029
                     /locus_tag="IWL52_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9779163.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1648..1989
                     /locus_tag="IWL52_002662"
     CDS             1648..1989
                     /locus_tag="IWL52_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9779164.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2008..2298
                     /locus_tag="IWL52_002663"
     CDS             2008..2298
                     /locus_tag="IWL52_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779165.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            2490..2723
                     /locus_tag="IWL52_002664"
     CDS             2490..2723
                     /locus_tag="IWL52_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779166.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
BASE COUNT         1092 a          380 c          592 g          865 t
ORIGIN      
        1 aacgtagcct tttacgtcat aagtgttaaa agaaataatt aatttcaaat ataaaaaagg
       61 gaacgtatgt ttgcatactc cttttttttg agttataata atacgcgaag agagataatt
      121 tcattattta aacaaactaa aaatcttgat attacttctt ttcaggcaat acgaatatta
      181 ttcgcttaca tttttttgta taaatttagt tgttttaaat ttaattgtct gctatgcctg
      241 tgatgaattg attttcgttc tgcacttttg gacgataaat ttggacaatt gaaaatacaa
      301 aaaaaaagaa aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg
      361 gcattaagat ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg
      421 atttagtata acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa
      481 atacgtgata ttacgatgaa tctatgccta agcataatgt tataaaaatg tccgtgtggc
      541 tctttgccgt atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct
      601 tttttgtttg tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt
      661 gttaaaaata tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt
      721 ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa
      781 ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac
      841 aaagtctcag aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat
      901 cctatcccct taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta
      961 ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgttta
     1021 atcagctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
     1081 attgtcgaga cttctaaaag aacttagaat cattagaaaa ataaggagta gacttgatct
     1141 atcaacaatt tccttcgggg agtgacgggg tagattttta cgattggtaa cgtaaaaaat
     1201 tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata
     1261 tacacagagt aaacaagagg gaatttagag gttagacaag ttagaatagc gcctagatta
     1321 atcatgagca gctactaagg gatctaggct ttgacgcaaa ctcatggaat aaggagtagg
     1381 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
     1441 acttgcgagg acgttggtca ttgggtcaat cgagcattta ccgctgtcta ctagggtttc
     1501 tcgtttcgga cgccataaac gagtcgatat tttttatgta tttttaagta aacgaaaaac
     1561 gataaaatat gaacgagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat
     1621 gaaatttacg taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc
     1681 acacatacta ttaaggtttc tttccaacgt tgggattaca aaggttttgt gacgtttaga
     1741 agaggcggaa attgtaaagg attagatgtt ttagcattaa acgaggatga tttatacgat
     1801 caaacattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
     1861 gaatggttta aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact
     1921 tggtcatatt tgagtgatta tattgtcagc gttgaaatca ttgaatttgt agcggataaa
     1981 gaagaataaa aactggagga ataaattatg aaatttttcg aagtaaaatc accatactat
     2041 tatgcgttaa tcgcagcaaa gaatgaagaa gaatgtgcag aattgtatga acaagtagta
     2101 acagatatcg aaaatcgtga agaatttatt cgatgtttgc atgagttaaa tcgaaatgaa
     2161 gcaattcaag aggatgcgaa aaaaattagt gaagaaacgt tacaaccaat cggtatggag
     2221 gaagctacaa aagacatttt tacaattatt gatgaacaaa aatcttgtgt attaagcatt
     2281 gattctacat tggtttaata gatccaagat tctataattt ttttcaaagt aaaaagaaat
     2341 taggtaatga ttctcagacc aattacttag gaaaataacc atacaaatta gattttacag
     2401 ataaaggatt agaaggaatc acggcgatta ataaaaaggt agtattacct taggcattga
     2461 gtaaggaatt tatttaggag gaaaattaga tggcgcgacc agttttagat gcgatggata
     2521 tggatacttt gacaaaagga aggtatgtac agctgttagc tcgtggttat tcacctaaag
     2581 atgtattcaa agccctatct aaaggaacag atatggaaaa agaaagatta cgaaaagaat
     2641 tcgactattg gagagagcat aacggaataa aagatttaaa accagcacgt ccagttatta
     2701 gacgaaaaaa attgaagaag taagaccaaa gcgtatggcg tacataggct tttttatttt
     2761 gataaaaata atgaaaggaa ggaaaactca tgaattgtaa agaaatcgaa aatagaaaaa
     2821 aagtgtcgaa agaaatggaa gaaaagttac taaaaacaat gaaacaaaaa caatgaaaca
     2881 aaaacacctt aaaagattat ctgtcgcgca atatatcaat gatatgcag
//
LOCUS       DACDVT010000149         2928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDVT010000149 DACDVT010000000
VERSION     DACDVT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.153"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(69..431)
                     /locus_tag="IWL52_002665"
     CDS             complement(69..431)
                     /locus_tag="IWL52_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9779167.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            654..1406
                     /locus_tag="IWL52_002666"
     CDS             654..1406
                     /locus_tag="IWL52_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9779168.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1546..2469)
                     /locus_tag="IWL52_002667"
     CDS             complement(1546..2469)
                     /locus_tag="IWL52_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9779169.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2484..2756)
                     /locus_tag="IWL52_002668"
     CDS             complement(2484..2756)
                     /locus_tag="IWL52_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779170.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          940 a          560 c          479 g          949 t
ORIGIN      
        1 tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa gatgattatt
       61 agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat atctttattt
      121 ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac atggactttg
      181 acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg
      241 atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc taccaatagc
      301 agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt cccaaaaaag
      361 aattccacta ctttaggata aaatgtctta atatttaaat tactattcac aggtgtttcg
      421 attagcttca tctcattcct cttttctata tagtcgattt agatccaatc ttaagttatg
      481 ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag acaaaaggat
      541 ttacctttta tcacttaata gtataacata atttcgtgag aaaaaaatac aattttttct
      601 tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa ataatggagt
      661 acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg cgcttttatg
      721 atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg atttatggca
      781 aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca ttcaagttgg
      841 atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct ttaatcaagc
      901 atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca actgtccaac
      961 agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt
     1021 ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt cgacaaaaat
     1081 acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat aaagaaacgt
     1141 atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt tacttaaaag
     1201 aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag tggttctctt
     1261 acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg tacgaagccg
     1321 atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca aaagctttaa
     1381 atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa
     1441 aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa cttttggtgt
     1501 ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc gttgaataaa
     1561 cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa atgccatttc
     1621 tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa
     1681 attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca agatgcctgc
     1741 tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa aaaagaagcc
     1801 tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt catcaaaaga
     1861 tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta gcctgccact
     1921 tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag taagaccttc
     1981 tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct tgatttcttc
     2041 acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg tattggcacg
     2101 agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat accttcgctc
     2161 tttttcttta tgatagcccg tcggtaaaag tgtcactttg taatcataat caaacgctgc
     2221 ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag gcagaatcac
     2281 tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag ggatataatc
     2341 gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat atggatgtcc
     2401 attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga tatctcgaat
     2461 gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata gtctaccaca
     2521 gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact gatccgctgc
     2581 ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat ttcttttact
     2641 atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt gaactcgaca
     2701 aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt attcattgca
     2761 agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc aaggagtttg
     2821 ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt attttaaatt
     2881 gaatacatta taatgatact gtaaatatat ctttattatt agtgaatg
//
LOCUS       DACDVT010000150         2884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDVT010000150 DACDVT010000000
VERSION     DACDVT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.154"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..801)
                     /locus_tag="IWL52_002669"
     CDS             complement(<1..801)
                     /locus_tag="IWL52_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9779171.1"
                     /translation="MDIFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQ"
     gene            complement(1027..1320)
                     /locus_tag="IWL52_002670"
     CDS             complement(1027..1320)
                     /locus_tag="IWL52_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9779172.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(1457..1636)
                     /locus_tag="IWL52_002671"
     CDS             complement(1457..1636)
                     /locus_tag="IWL52_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779173.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(1633..1983)
                     /locus_tag="IWL52_002672"
     CDS             complement(1633..1983)
                     /locus_tag="IWL52_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779174.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMTTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1976..>2884)
                     /locus_tag="IWL52_002673"
     CDS             complement(1976..>2884)
                     /locus_tag="IWL52_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP9779175.1"
                     /translation="RRKKLAQMIQERIKEELGLIATVGVGDNPLLAKLALDNEAKHNE
                     GFIAEWTYKNVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKSRL
                     GVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQV
                     AIRIRQHNCKTGCVHLDIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEG
                     QEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEKLDKIIDKIRQKYGFTSIVHASSM
                     LEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT          840 a          554 c          446 g         1044 t
ORIGIN      
        1 ttgtctagca tctagttctg ccaaaatatt gataaatact tggtgtgctt tataatccca
       61 ttgatcaaaa tttccatttt tgtattttct taaatgtata ccaattttag aataccttct
      121 caatcttttt tgataatgaa tgagattttt ggataccact gttccgtatt tttcaattgc
      181 ttcttctagg atttcttcat caaaatcatc atttggttct accatacaag gtacaatatc
      241 tataatttgc aaatcaagat cgtatctatc gaccatgaat ttttgataat ttacatattt
      301 gttgactccc tctaaggatt cttcagctgt ttgaaatgaa attatcgaat agtctgaagc
      361 tgccatagca ttatcggaaa attcgctaat ggttggtggt acatcaataa atatttcttg
      421 ataattgttc tttattggaa gaagtaattt tttcaagaca gtaacttggt ctaattcatt
      481 ttcatgttta ttagcaaaag cggagaatga tcgaaaacta gtgtcacatg ccagaaggtc
      541 aagattttca tttaccttaa taatactact tgctaattct ccatttgcga caccatttgt
      601 tatagtggtt ttgatatcgg ttataccgaa agttcttgct atgacttgtg ttaagttcgc
      661 ttgagggtct aaatcaataa taagagtttt tctatttctt atcattgcat tgtcataagc
      721 aagcatgctg actatcttag ttttacctac tcctccttta aaattgccaa ctgtaaatgt
      781 agtcccttga aaaatatcca tgtaaatcct cccaatcttt aattgataat taaagtataa
      841 cttatatatc ggaaatggtc aacattaaaa gtataactta aataataata tctaaaaacg
      901 tttggatata gcggttttta agaataaaag tataagttat accatatata tttagggtat
      961 aacttatact tttaatattt aaggtataac ttatacctta aatattgctt ttcgaaaata
     1021 gctattctag tctatctgtt tctttacttt tcgctgctga tttgaagtga attgatcata
     1081 ttcattttct agtattaata acttttcgta tacatcggtc atattcatac tagaagcaag
     1141 acttagatca tatacaaatg aagcaggtaa tgattttatt ctacgttgtc gggttatcca
     1201 gcttgcaacc gttccttgtg ggtaggcata taactcacaa aattgttcta cagtgattcc
     1261 taaattcttg ataatgtaaa cattgatcgg gtgcgagtaa acaaatgtat tgatagacat
     1321 ggtgcgtaag cctactttct tttattattg cgtttgcaat aatagtttaa cacacgcatt
     1381 attctagtat ctactcctat ttgtaaaaaa caaaaaagga ttcatagaga taactctata
     1441 gaatccgtaa aataccttat ataaataacg ggaaaatata aaacctaaaa ttatgataac
     1501 ctgctgcagc ttctacatag aaagagaagc gctcaacacc aacctcattt atcaaacgtt
     1561 ggccataacc ccaaatctct tctgatagct gatcaaaggt tatatacttt agctcatatt
     1621 gctggataat gttcattcta atcgactcca tttattatcc tggtaaaatt cgatatgacg
     1681 tatttcgtcg tagtgaattt tgtctttgcc aacgtagatc cccagctcat cactacctaa
     1741 tattttgcca attatatcgt ctggatagta tccttcagca tttcgttctt ctttttgaat
     1801 acatactaat ctatttttta tgactgcagc gttcaatact tccgaaatct cttcagtcgt
     1861 catttgttct tttgctaagt tttgattggc tcgctgttca ttgtccgcat cgatctcttc
     1921 cgtgtgatcg gataaataga atcctgccca tttcaatcgt cctcgatcgt tatagtcaat
     1981 catttttaag cccccctgcg ccgcctgcat ggccaccaac caacgtgctt ctagtaatac
     2041 tacgagcgcc ttctaacatc gaagaagcat gtacaattga cgtaaaacca tacttctgcc
     2101 taattttatc tattattttg tctagttttt cgtcatcaat ctgtttttgt ggatcactaa
     2161 acaagtccag ctgaagcgaa tcagtataaa ctagcttgct gtaagtgatt ccgacgtggc
     2221 ggacttcttg tccttcataa tacttatcaa acaagaaaag acaataattc tgcaattcct
     2281 ttgtattgtc tgtgataggg attttcatct ggtgtgagaa ccctggtctc gtttctaaga
     2341 tcgatgtacc aatgtctaaa tggacacatc ctgttttaca attatgctgt ctgatacgaa
     2401 tggctacttg ttccgccatt tcttttacca ccagctcgat ttcatttctt ctagtataat
     2461 cacgtggcaa cacttgtgaa ttaccatagg acttttcttt tgtcggttct ggtgggattg
     2521 ctatatcagt tcgatcaata ccatttgcat gaaagtatag ctgtaagcca atcacaccta
     2581 aacggctctt aatggtgtat ggattccagt tagcaagatc tctaatgctc ataatcccca
     2641 tctgattgag tcttttcttc attcgagagc caatgcccca aaagtccgtc atttcaggaa
     2701 tattccatac tttttcagga acgtttttgt acgtccattc cgcaataaat ccttcattat
     2761 gctttgcttc attgtctaag gctagtttag ccaaaagggg attatcgccg acgcctaccg
     2821 tagcaatcaa acctaactct tctttaatac gttcttggat catttgagcg agcttcttcc
     2881 ttcg
//
LOCUS       DACDVT010000151         2813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDVT010000151 DACDVT010000000
VERSION     DACDVT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.155"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..1141
                     /locus_tag="IWL52_002674"
     CDS             14..1141
                     /locus_tag="IWL52_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9779176.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="IWL52_002675"
     CDS             complement(1359..2501)
                     /locus_tag="IWL52_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9779177.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="IWL52_002676"
     CDS             complement(2506..2691)
                     /locus_tag="IWL52_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9779178.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          929 a          516 c          447 g          921 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctt
//
LOCUS       DACDVT010000152         2790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDVT010000152 DACDVT010000000
VERSION     DACDVT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.156"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(244..1218)
                     /gene="sgrA"
                     /locus_tag="IWL52_002677"
     CDS             complement(244..1218)
                     /gene="sgrA"
                     /locus_tag="IWL52_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9779179.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1414..2253)
                     /locus_tag="IWL52_002678"
     CDS             complement(1414..2253)
                     /locus_tag="IWL52_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9779180.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2438..>2790
                     /locus_tag="IWL52_002679"
     CDS             2438..>2790
                     /locus_tag="IWL52_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9779181.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQML"
BASE COUNT          874 a          606 c          412 g          898 t
ORIGIN      
        1 aacgtacaaa tagattcgtt actgattttt cagagatact accatgcgca gtgtgcattg
       61 tcaattattc ttgctcttat tttccatttg tgttcctgtt atttcctgtt tctcaaatat
      121 gttagtaaac aaaaatagat ctggcaaagc tacaacttcg ccaggcaata cagcactctc
      181 gaatgaaata ttcactcaaa aatctatcgt tttattcaat tttagatctg ttgcttttat
      241 tctttatttc actcgatgga agagagaacg aaaatagctt gctcctacaa tcaaaatgag
      301 taacacaccc atccatgtaa ttagcttgtt caaggttcta ctaccagttt ctggtaaatc
      361 gctttttcta ttatctgttt ctttcgcttt ttcttgatga tcaattccac gagaaacaaa
      421 atcaggctgt tttagcacac tttttaggtt gctccctgca tttctctttt gttccttagt
      481 tggtatgata ggtaccaaat caagttcatt atttactttt cctgtgctgc tttcgctgct
      541 cgtgcttcct gtgctactct cactactcat gcttcctgtg ctactttcac tactcgtact
      601 ctccgtactg ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg tactctccgt
      661 actgctttcg ctgctcgtgt tttctgtgct actttcactg ctcgtactct ctgtactgct
      721 ttcaccgctc gtgttttctg tactgctttc actacttgtg ctatccgtac tgcttggcgg
      781 tccaacaact gtccaactgc ttggtttcaa attctccgtg atcgtcatct gtccatcata
      841 aaaaacaatc aaatcaactt tatctttact cttgatctca tcaatcgata gttctgcaga
      901 aatcattatt gtatactcaa taccagcggt ccattgtacg tcttttaaat aaacttcccc
      961 aataactttt tctgaacttc cattaccatc ccaaccatat gttagaacag catgtacatc
     1021 actagcattc acgcttttgt ttggtttgac tggaatagta atgacttgtt gtgtcatgtc
     1081 ataaacaatc cgatcagttt tcacagtaaa tgaaagattc tcatttgccc gttcatttgc
     1141 agaagtactg attccaatca ttgctgcaat tatactcagc ccaagaataa caaataatac
     1201 ggtcgctgtt ttcttcattt ctatcactct ccatataatt accttctatt tgataaagga
     1261 attatataaa atgaaaatga tgagaacacg acagtcagtt gtgaaaatct aacaaaagat
     1321 gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat caatgacaga cttaaaaaag
     1381 caagaccgat atcagccttg ctttcttcat cttttattga taaacagcag tgtatttctt
     1441 catccgcttt gttttatatg catagagtaa caaaccaaca atcaagacta acaagaataa
     1501 tgtagttgat atagatgctt cagccccaaa gtttccccca gtaattattg tattttgcga
     1561 ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg ctgactggta tacccaaaat
     1621 agctccttgt gtccgattcc aagtaatatg agcaccaata gccgtaaata aacttcctgt
     1681 actgtagata agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa
     1741 tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat gcagcagatc caatcattgc
     1801 tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata aaagctcttc
     1861 tgatgccccc actgcaataa ataaaataaa actcgttatc agcggcacaa tgtttgcgtt
     1921 tcctgaatct aaccgatacg cccccgtcaa atataatgga ctagtaataa gcagaaacac
     1981 tccaattcca acaacgagac ctcccgctaa atacccccag tcttttcctc gaacagaaac
     2041 accataatct tctattcttt ttccttcgac cttactagca agaaataaag ctagtaaaaa
     2101 aacagctaaa tgtccgaccg taccggcaat tgctccaatc cattcactat ttccaataaa
     2161 catatccgca agtgcattat tgacaagaat tcctaaaaca aaaccaacta agactattaa
     2221 aacagatcca atcaaccgaa taatcctgat catctttcat ccctccttta tattaagttt
     2281 aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa tgttctccat catagagaga
     2341 atagattttt acgaactaag aatatctaca gcaaccttac gaaaatgaaa gactgtctcc
     2401 ctaattcttc tgttagactg tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca
     2461 actactaatt atgtgccttt cagcatgtgc aattttacta ttctcaggtt gtagcaagtc
     2521 caatgaaacg attgcaacgt ttaaaggtgg aacacttaca gttcaggatt tttatgataa
     2581 cccaaaagtt aaatcaaata atcaaacact attaaaaaaa atgattgtct tcaaagcatt
     2641 tgatgatgta tacggaaaag agatttcttc aaaggaagta acaaaagaat ataatgaaca
     2701 aatcaaaaaa ctcggtccaa actacaagga acaattaaaa gcagttggcc aaacagaaga
     2761 gacatataag aaactcttta aacagatgct
//
LOCUS       DACDVT010000153         2729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDVT010000153 DACDVT010000000
VERSION     DACDVT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.157"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..357
                     /locus_tag="IWL52_002680"
     CDS             <1..357
                     /locus_tag="IWL52_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9779182.1"
                     /translation="DGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGT
                     TLSGYTEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGA
                     ITRPQEIASRFVKALS"
     gene            371..1873
                     /locus_tag="IWL52_002681"
     CDS             371..1873
                     /locus_tag="IWL52_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9779183.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            1886..2362
                     /locus_tag="IWL52_002682"
     CDS             1886..2362
                     /locus_tag="IWL52_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9779184.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2459..2569)
                     /locus_tag="IWL52_002683"
                     /pseudo
     CDS             complement(2459..2569)
                     /locus_tag="IWL52_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          900 a          365 c          532 g          932 t
ORIGIN      
        1 gatggactat ctataaattc ttttattact aaaataaaag aaaaatatcc tcatcagtta
       61 tttatggcag atatttctac atttgaagaa ggcaaaaatg catataaagc aggaatagat
      121 tttgtaggaa ctactttaag tggatatact gaggaaagta aaaaacaaga tgggccagat
      181 atagatttga ttaaaaaatt ggtagaagaa gggatttgtg taattgctga gggaaggatt
      241 catactccag aacaagcaaa agagataaaa aaaataggag ttgcaggtat tgttgtgggt
      301 ggtgctatta ctcgaccaca ggaaattgca tcaagatttg ttaaagcatt aagttagggg
      361 gagaaataaa atgtttaaaa aattttcaca attaggacgc gctttcatgt taccaattgc
      421 tatattacca gtagccggat tactattggg actgggagga gcgttaacaa atgagagtgc
      481 tataaatgct tatccatttt tagatcaacc gtggttgcat acaattttaa gtattatgag
      541 ttatgctgga aatgcagttt ttgcaaacct agcactaatt tttgcaattg gtattgctgt
      601 aggacttgca aatggagata aaggaacagc tggtttagct ggtggagtag cttatctagt
      661 atatactgca actattagtg ggtttcttga acttttttca gcaaaagatg caacactaga
      721 tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt gcatttttac acaatcgtta
      781 tagaaaaatt gaattaccac aattcttagg tttttttgga ggatcaagat ttattccgat
      841 tgtttctagt ttagctgcaa tcattattgg ttctttcttt tatttaattt ggccaccaat
      901 tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa atgggtagtt taggaacttt
      961 tttatatggt tttttattgc gtttaacagg tgctgttgga ctacaccaca ccatttaccc
     1021 tttgttctgg tatacatctt taggtggtac cgaaactgta gctggatcaa caattgctgg
     1081 agcccaaaat attttctttg cccaactagc tgatccaaac cacactggtc tgtttactta
     1141 tggtactaga ttttttgcag gaagatttgc aacaatgatg tttggattac cagcagcatg
     1201 ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa aagaatggtg ggctttattt
     1261 tagcggggcc cttacatcat ttttaactgg aattactgaa ccagttgaat atatgttttt
     1321 atttgttgct ccttggcttt atgtgattca tgcattctta gatggattat ctttttattt
     1381 tgctgatatc ttaaatattc gtgtagggaa ttccttttca ggtggtttga tcgacttttt
     1441 attattcggt gtacttcaag ggaatgacaa aactaattgg ttaaaattaa ttccttttgg
     1501 tattgcatgg gcatttatat atttctttgt ttttacattc tttatcaaaa aatttaaggt
     1561 agctattcct ggaatggaag aagaatcaga agttcattct gaaaaagtaa taaacaaaaa
     1621 tacatcatta catgaagagt ctcttatcat tattgaagct cttggtggaa aagaaaatat
     1681 tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct gttacgaatg gggagattat
     1741 tcaaaaacaa acaataaaag acactggtgc aactgctgtc ttcgaagtga agggtggcat
     1801 ccaagctgtt tttggaggga aagctgactt attaagtcaa gaaatcaatc aaatattagg
     1861 aaaagataat taggaggttt ttaatatgtt tgaaattttc aaaaagaaaa aacaagaaac
     1921 tttttatgca ccttgttccg gaaaaatcat ttccatcaca gaagtaaatg atcctgtctt
     1981 ttcacaaaaa ttactaggtg atggttttgc cgtcatacct gatggttcaa aaatttatgc
     2041 tccattggct ggtcgagtaa caaatatatt tcctactaaa catgctatta atataaaaag
     2101 caactcaggc attgattatt tgattcatat tggaattgat acagttgaat tacaaggaga
     2161 gcctttttcg atttgtgttc aagaaaatga tgaggttact cctgaaacct cgttggttga
     2221 tgtggatttt gacaaaataa taaaatctgg taaagatact tctgttatag ttgtttttcc
     2281 ggagaaaaat cagttcaatg atttaataat agataatggt cgaattgctc atgggaattc
     2341 atgtggaaaa atcacttttt aaaaaagtgt atataaaaag ctataaaata agctaaatag
     2401 gatatttgtc aaatgaagtt ggtgaacaga aagaactgtt ttctttctgt tcattttttt
     2461 attttattag taatgattcc ccatgccaaa tcatgatccg taatttaaag ttataaaagc
     2521 ttcgaaatcc attggcattt cgttggaata ccttgatatg attattcaaa tataaaatat
     2581 agagagaaga gagagaggga agatatttat aaagggcaaa gttatgttta ctcaacttag
     2641 gaaatagaaa tagatcaatt tatattctct tgtcaatcga atatgaacct tagcactata
     2701 taccaaatat agtaaaatgc tgaaagatg
//
LOCUS       DACDVT010000154         2699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDVT010000154 DACDVT010000000
VERSION     DACDVT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.158"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..735)
                     /locus_tag="IWL52_002684"
     CDS             complement(<1..735)
                     /locus_tag="IWL52_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9779185.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLILCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            complement(786..>2699)
                     /locus_tag="IWL52_002685"
     CDS             complement(786..>2699)
                     /locus_tag="IWL52_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9779186.1"
                     /translation="TIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQ
                     ELNGKIAELKDSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAA
                     FTLLMPIIVGFMALGGPTTLIIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLG
                     GTWESIKEGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQ
                     ETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAG
                     QLLGAAWQAVWTPISDFLKNTWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAI
                     STTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISST
                     ASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISS
                     KVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFD
                     NLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVL
                     PLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDS
                     ITKVQNDNASAWR"
BASE COUNT          833 a          583 c          415 g          868 t
ORIGIN      
        1 ccttaaccca ttacgataaa tttgtatttt gctcaaacca gtacaattaa aataattaat
       61 atccacttgc aatgtcggat attgcatggt cttcattttg ttggaccgat ctaaaatatc
      121 tccgtccgat tgctcttcgt agcaaagtat caaatcacta tcaatgacta cgtcagttcc
      181 tactactaag ccttcaaaac taaacacata atcatttaag atgaactggc atgaagtagc
      241 tgaaggagtg atgataatct ttggaaaact ttcttctaaa ctattattca gcaagttaaa
      301 tgactgtggt ttatctacgg ttataggtac atcttcttga actcttgcga atggtttcgc
      361 agtaatattt acatcgaact ctccccattc aacaatatcg ttttctgcat ccccaatatc
      421 gatagtctgg ataacataat agacgttggg atcatcagag aattctaatt tctttgcata
      481 atttaaccaa tgacgcatga tataaaacga ttgcttgaac gcttgatggt cttccacatc
      541 ctctaaatag ttatagtgca atgtaaacga catgtcttca aacgagtaat cttgtactaa
      601 gccacctaac cttcctaaaa cagaagtttc aactctctgt ctttttggag aaggtatggt
      661 tggtctttca gctaaagcca atttatgcaa ataatcagga aatccatcga ttatagaatg
      721 tatacaatca gtcatttttt cacatccttt ttaatactaa aaaaacagga gaaatactct
      781 cctgtttaac gccatgccga agcattatca ttttgaactt ttgtaatgct atcaatgatt
      841 tgttgagttg tttgcttcat agtaacctca tctgcgttac catcaattgt gaaattgaat
      901 tcgtagttat tcacaggttg aatcgtttgt gccctagatg aaactgatgt gctactcaag
      961 atacgatcac caatttcttg cagcacagat cttttcaaag gtaaaactgc ttcaggtcct
     1021 gcttcgccga caccgataat attcggagaa ttaaacacac tacctttcgc ataccaatca
     1081 acacccaacg ttgggatttt ccccttcaat ggattgaatt ctccgctcaa tttaaaatgt
     1141 ggtaacggaa tatgtggtat agaaatattc aaattatcaa agatactact gattttatct
     1201 ctgatccaat caattggagc gctaacagtc tttttgatac cttcccaaat gttagcaatt
     1261 gtacttttaa cattattgaa tatgtcggaa acaatacctg ttagattgga ccaaccgctt
     1321 gaaattgcat tttttccatc gtttacttta gagctaatag tgcttgtaat tccattccaa
     1381 agattcaaag cagtgttttt gataccgttc caaattccgc tgatccacga agatatacta
     1441 ttccaaacac tttgaatggc acttttagct gcgtttatag cattgcttat actactagtc
     1501 acgctattcc agatatttga tgctgtagag ctgattgaat tccaaattcc acctaaccaa
     1561 ctagatacag ttgaccaaat attttgaatt actgtagcag ctgcttgtac caagctagtg
     1621 attgtattct tgatactgtt ccaaatacta gaagctgttg cactaattga attccaaata
     1681 tttgaagccg tagtactaat agctgtccat ataccattcc accatgccac tactggatca
     1741 aatatagtat ggaatgtagt tacaattcca ttccaagcga tgcttaccca ttgtgtcata
     1801 gtatcccaag tgtttttaag gaaatcagaa ataggtgtcc aaacagcttg ccaagctgcg
     1861 cctaataact gtccagctac atcaaaaata cccacgataa tattaatacc ggcttgaatc
     1921 aatgacgtta ttaaggtcca tggtatttga acaattccta caatgtctgc ccaaataatc
     1981 gaccatactt ctttgactcc gttccaaata tttgaaaccc aatcaacgaa tgcttgccaa
     2041 gtctcttgga ctccttgcca gatgttggaa gctccttcaa ctaatccgct ccatagctct
     2101 ccaaaccaat cagaaactcc ttgccaaata tcttgaaccc aatctacaaa tccagaccag
     2161 gtttctttaa ctccatccca aactgatgag gcaccttctt ttatactttc ccaagtacca
     2221 cccaaccaat cagtgaattt actccatatt cccttaaacc agtcagtaat tgcgccccag
     2281 ttttttataa ttgctataac tccagcaata acagtaataa ctgctcctat tattaatgta
     2341 gtaggaccac ctagagccat gaagccaact attattggca ttaataaagt aaatgcagca
     2401 gtcaatccgc caatcgctac ggcataatcc tgtactggtt gtggaagatt attaaacgca
     2461 tcagccatct ttcctagaaa atcaattact ggttcgagtg catctatgat tgtgttgcct
     2521 ataggagcta atgaatcctt taattcagct atttttccgt ttaattcttg taacggagta
     2581 gtagaatctt cattcatttt ttgtgcagat ccactaacat catcaaatgt atggttaaca
     2641 tcagtcaaag attggacaac tttcatcgcg ttatcttcgc caagtgcaga ccaaattgt
//
LOCUS       DACDVT010000155         2609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDVT010000155 DACDVT010000000
VERSION     DACDVT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.160"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1562
                     /locus_tag="IWL52_002686"
     CDS             <1..1562
                     /locus_tag="IWL52_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9779187.1"
                     /translation="YFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTL
                     VFNRYLSLMGRGNGKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKV
                     IKDHPDLDKKVFKATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYS
                     MINVATSGGGKIRDYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSL
                     FPFICRLDNDNEVDDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRM
                     NRPMEDTRFAVASYDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVY
                     FTQHTFIHESALRLQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIK
                     KIAMDMYRAKILKPALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMR
                     WYCMNVYEEHISNGNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVE
                     NTGTGFTPLVF"
     gene            1582..>2609
                     /locus_tag="IWL52_002687"
     CDS             1582..>2609
                     /locus_tag="IWL52_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9779188.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGV"
BASE COUNT          924 a          369 c          520 g          796 t
ORIGIN      
        1 tttacttcga taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat
       61 tcgaattaat tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa
      121 aagataaaac actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa
      181 ctggttttgc ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt
      241 atgatattga tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat
      301 ttaaagtaat taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag
      361 ttattcaaaa tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa
      421 aagatgggaa gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt
      481 caatgataaa tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg
      541 atacaactaa tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa
      601 tgattctttc tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc
      661 catttatttg tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag
      721 cttgtccaac tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc
      781 aaatgcaacg taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta
      841 tggaagatac acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag
      901 aatttcctga aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt
      961 ttgccagcgt tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt
     1021 ttatccacga atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta
     1081 tagatcaagg aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag
     1141 gttggtttct tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc
     1201 gtgcaaaaat attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa
     1261 gcggatctgt tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac
     1321 gtttattttt tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc
     1381 atatttctaa tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg
     1441 gctttttttc attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg
     1501 taacagtcac aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct
     1561 aacttgaaag gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa
     1621 aaagcaacgg ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga
     1681 aaatttgcga ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc
     1741 aagtcatatg aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct
     1801 cctaataaga aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc
     1861 tacaatgaag tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat
     1921 agttgggatg tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac
     1981 aacatagtcc ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat
     2041 caacaaatta ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt
     2101 gccatgaatg tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc
     2161 cgaccgcaag acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag
     2221 gcttttatgg aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta
     2281 gaagatttca gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac
     2341 atctttgaga tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg
     2401 agtgggttat cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa
     2461 cttattcagg atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat
     2521 tttgtacgtg tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct
     2581 attgatgtag gcattagaaa tggagtatt
//
LOCUS       DACDVT010000156         2572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDVT010000156 DACDVT010000000
VERSION     DACDVT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.161"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            484..1038
                     /locus_tag="IWL52_002688"
     CDS             484..1038
                     /locus_tag="IWL52_002688"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779189.1"
                     /translation="MDVLCGKINPAGKLTITIPRHLGQIPIYYNYFNTGRPYSSEKFN
                     TTKYLDVSNEPQFPFGYGLSYTTFSMSPISIKEKGKNCLKVTTTVSNKGSRDGYEVVQ
                     LYIGAQYGRYIRPTKDLVAYKKVFVKSGDSVTVSFELTNKDFKYYDEEFNEIIDDGAY
                     WIMVGNDSSQALTNKEEIEVRINE"
     gene            1031..2446
                     /locus_tag="IWL52_002689"
     CDS             1031..2446
                     /locus_tag="IWL52_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9779190.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKNKVLLQSFK"
     gene            complement(2476..>2572)
                     /locus_tag="IWL52_002690"
     CDS             complement(2476..>2572)
                     /locus_tag="IWL52_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9779191.1"
                     /translation="ILGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          775 a          409 c          530 g          858 t
ORIGIN      
        1 tcatttcagg taatctcaaa taaaaaaata tacagtcctg tggcacttta cctatgggac
       61 tgtatatttt ttactctcag cgggcttgtt ttgactccgt gtctgactcc gtcgtattga
      121 ttttaaatga tattcgatga ccatttaaca ggtcattttt gaaatttttg tctctatatg
      181 actcagtttg ataattattg atagatcaat ttgggtctat atcgaacaag ggaataatcc
      241 aataaaaaat gatttagaat atcaaaataa caaaaaagag gactacctgt attcgtcctc
      301 ttttttgacc agattttgac cagtctaatt tacattctaa tatattcata tgagctaatt
      361 ttttgattaa atggtaagtt tttccttgtt tgttttagtt taaactagtg tgtaatttaa
      421 aaatctatat gtaagggatt agttgaggct tggcatctag gagcagaggc tggcaatgct
      481 attttggacg tcctttgtgg caaaataaac ccagccggaa agcttacaat aacaattccg
      541 agacatttag gacaaatccc tatttattat aattatttca atactggtag accgtattca
      601 agtgaaaagt ttaatacgac caaatactta gatgtatcca atgaaccaca atttcccttt
      661 gggtatgggt tgagttatac cactttttct atgtcaccaa ttagcattaa agagaagggt
      721 aagaactgtt tgaaggtgac tactaccgtt tcaaataaag gaagtagaga tggttatgag
      781 gttgttcaac tatatattgg agctcaatat ggtcgttata taagaccaac aaaagacttg
      841 gtagcctaca agaaggtttt tgttaagtca ggggatagtg tgactgtttc ttttgaactt
      901 acaaacaaag attttaagta ttatgatgaa gagttcaacg aaattataga tgatggtgct
      961 tattggatca tggtaggcaa tgattcatct caagcattaa ctaataaaga agaaatagag
     1021 gtaagaatta atgagtaata aagaagaagc taaatcaatc attaactttg ttggtggaaa
     1081 agagaatatt gcagcactga ctcactgtgt gacacgtctg aggtttgcct tgcgagatga
     1141 cactaaagtt gataaggcag gattggaaaa cctggaagat gtgaagggta ccttcaatgc
     1201 taatggacag tttcaagttg ttatcggtcc taaagtagca gatatctatg aagaggttat
     1261 aagccaatct gggctgagtg gggcaaacaa agaagacgtt aaaaaagtag cggctgagtt
     1321 gaatttgaat tggtttcaac gtgcagttaa aatattgggt gatgtatttg tgccgatttt
     1381 accagcgatc gttacagctg gtttactgat gggactgaat aatttattaa caaacccagg
     1441 aatctttttt gagcagtcac tcattgaagt atatcctaat attgctgatg tcgctgatat
     1501 gataaatctt atcgcaaaca cttcctttca atttctgcct gcgttaataa catggtcaac
     1561 tgtaaaatac tttaaaggaa atcctcttct tgggattgtg ttaggtctag tctttgtaca
     1621 cccagatttg atgaatgcct ggaattacgg gcaagcagta accacgggag atattccaca
     1681 ttggacaatc tttggattgt ccatctctaa gataggacta caagcacaag tattgccagc
     1741 tctagtttcc ggctatgtgt tagctaaaac agagctattc ttgaaaaagc gtgtgcctga
     1801 aacaatccaa ctgttggttg ttgcaccagt tgcaattctt tttactagtg ttataacatt
     1861 tattattata gcgccgatta caattgctat tggtaatacg attgtcgcag gtattgtatg
     1921 gttattccaa aaagttccat ttattggcgg atttgcttat ggtattggtc ttgcgccatt
     1981 agtcgtgaca gggatgcatc atgcgttttt accagttcat attcaattga tggcgaacgg
     2041 tggagattat atttggccta ttggagtcat gtcaagtttg gcacaaggtt ctgctgctat
     2101 tgcaatgaat ttcttaatcc aaaataaaaa gttgaaaggt gttggactgg ctgccggttt
     2161 gtctgcttat atgggtatca ctgagccagc aatgttcggg gttaatctaa agtataaatt
     2221 cccattcgtg gtttccattc tgacctctgg aataattgga ggatttatgg cgtcacaaca
     2281 cgtcattgca acttctgttg gtatcagtgg aattcctggt ttcttgtcta ttccgtttga
     2341 attctggcca atattcttta tttgtatgct agcaagttgt gtcgtctcat ttggagcaac
     2401 ttacctttac gggaaaaata aggttctgtt gcaaagtttt aaataaagaa taaaatccct
     2461 tacggtatct atgatttaag ctgggattcc caataatacc ttgatttcag tacagaccga
     2521 aaacccgaag agagtgcctt cttttcgggt tttcttatat aatcctagaa tg
//
LOCUS       DACDVT010000157         2488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDVT010000157 DACDVT010000000
VERSION     DACDVT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.163"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..500
                     /locus_tag="IWL52_002691"
     CDS             78..500
                     /locus_tag="IWL52_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779192.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            520..2112
                     /locus_tag="IWL52_002692"
     CDS             520..2112
                     /locus_tag="IWL52_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779193.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMENGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKVAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
BASE COUNT         1005 a          391 c          498 g          594 t
ORIGIN      
        1 gttttggaag catacacgaa cgaggtcaag aacttaattg aaatattaat cacacattag
       61 gaaggagata atgttctatg gttaaagcga tagaaaagca tattacattt tatgaagcgc
      121 acgatgaagt tttaaaacat attatggaaa ctcaacctac ggtaaaaaat tattctgaag
      181 ccattcgatt tttatgtatg aattacgatc cagaaattga aaaaaataat aatatgacga
      241 acgcaaagtt gaatgcaatg ggaaaggaaa tttctatttt aaccgaaatg gtttcagagt
      301 ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca actgtctatc
      361 aagaagcaaa agagcgtgtg gaagggaaaa taaaagcgaa tcaaacgaaa aagtattctc
      421 ctaagatcaa gaaaccaata aatgaatctg ttcatgccag agtagacccg tttgatccaa
      481 caaagttttt aaaaagataa aaagtagaag gggaacatga tgaataaatc gcctgcagtc
      541 attctcactt gtcaatttac cttgcccaat aaaaaatctt tttcaacata cattgattat
      601 atgacaagag aaaaagcgct ggaagaaaag gaacgacgca cgcccgacga agaacaggaa
      661 ctgcaacgaa tcaagaatgc tttagaggat ttttatattc ctgaaggaga aactttttcg
      721 gagagtaaga cggataaaga attcagcgaa acgtcgaatg aagctcaatc gttgatggaa
      781 aatggaatca actttgacaa gttagaggaa caagatttta ccaaatatct ttcttatatg
      841 acccgtcact atgcgttaga acaaaagaaa gaactgacgc aaagtgaaca aaaagaatac
      901 caacgcttga accaagcaat aaaaaaatat caaacagagc cacacaaaaa ggcttcagga
      961 aaccttcctg gagttttttc tatggcttca gacgaagtga aaggaagtga tctgaaggag
     1021 attaagcaaa tcttccaaag aggacaacaa aaaggctcaa tcatttatca agatgttgtc
     1081 tctcatgata attcgtatct tgaaaaaata ggattgtacg atcccaaaac agatacttta
     1141 gatgaagaag gattaaaagt agctggaaga aaaatgatgg agacattatt cgagaaagag
     1201 caactgaacg aaacaggcta ctggatggca acgattcacc gtaataccaa acatatccat
     1261 attcattttg cagttgttga aaaagaaaat acgcgtaaac cttacacgga ggttgaaaat
     1321 ggtgtgagtt atatcgtacc gaagggtaag agaagacaag caacattaga cgcaatgaag
     1381 accaattatg tacatgagtt agaacgtttt tcttatgaaa aggatcgaat gattggtatg
     1441 gagaaacgaa atttattgac gcggaaaagt gatttgcgta acaccttaac aaaagtggta
     1501 aaagagccag cgcgttacga tcgacacgca atgaacttat taaatgaagt atataaaacc
     1561 ctacctgaca aacgttcgga atggcattat ggggatgaaa aacgaaccaa actttcacca
     1621 tacacaagag agaaactcga tgagttaacc aaatatgttt taacaaaaga acctgaatat
     1681 caagaatata tacagatttc aaaagcattg aaagaagaag caaaaagtct ttatggggaa
     1741 agcaaaagag aatcaaagga tgcagaaaaa aatgctctct ttgatttgaa aaagcgcaca
     1801 ggaaatgcca ttctaacaga actgaagaaa cagaatagtc aaataaaacc tttaatagaa
     1861 agtatggatc agatgcaatc tatttacaat gaacgatcct ttcgaacgct taaacagtta
     1921 ccaacgaatg aatacaaata tagttacgaa gaattcatga agcgcaggaa acaattccaa
     1981 caaagagttg tcacgcaacg gagtttgcgt caattaaatc gaacgattaa tgcccatgaa
     2041 gaaaaatacc acgcaagtaa agcctacgaa gagcaacaac gaagaatcca aatagaacaa
     2101 gaaagacagt aaagacagta aagttagcgc cttattatac tacttgggaa atgaatgaag
     2161 aggggatcaa taacttcccg tgtaaaagca ttgaagagtc tatttcactc acagaatgtt
     2221 agttgttaag tgaataaaag aagtaaaaga gtcctgaacg taattgtcca ggactctttt
     2281 tttcaatgaa ttgttttccg ccaattcaat gagtggtgcg atttgcgact acattatatc
     2341 aaaagaaaga gaagggagga agaagaaatg gcttattcaa cgattggagt tgttgttgaa
     2401 aaaagcagag acaatctcgt ttttgtgaca gaaattcaaa caggcagagc ttttgtcgtc
     2461 acagacaaag cggcaaaagc gtatcaaa
//
LOCUS       DACDVT010000158         2481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDVT010000158 DACDVT010000000
VERSION     DACDVT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.164"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            352..1041
                     /locus_tag="IWL52_002693"
     CDS             352..1041
                     /locus_tag="IWL52_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9779194.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IWL52_002694"
     CDS             1028..2236
                     /locus_tag="IWL52_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9779195.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
BASE COUNT          805 a          428 c          490 g          758 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggatgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga a
//
LOCUS       DACDVT010000159         2475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDVT010000159 DACDVT010000000
VERSION     DACDVT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.165"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..418
                     /locus_tag="IWL52_002695"
     CDS             <1..418
                     /locus_tag="IWL52_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9779196.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IWL52_002696"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IWL52_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9779197.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IWL52_002697"
     CDS             1305..2459
                     /locus_tag="IWL52_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9779198.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          854 a          443 c          521 g          657 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat aattt
//
LOCUS       DACDVT010000160         2463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDVT010000160 DACDVT010000000
VERSION     DACDVT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.166"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..516
                     /locus_tag="IWL52_002698"
     CDS             34..516
                     /locus_tag="IWL52_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9779199.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            553..1563
                     /locus_tag="IWL52_002699"
     CDS             553..1563
                     /locus_tag="IWL52_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9779200.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1678..2391)
                     /locus_tag="IWL52_002700"
     CDS             complement(1678..2391)
                     /locus_tag="IWL52_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779201.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          839 a          405 c          500 g          719 t
ORIGIN      
        1 tctcaaataa taaaatagaa aagagacata aaaatgattg acttggcaaa gaaattctta
       61 cacgaaaata tatattttga gttgtatcat catcgagcca tttatacaaa tgaagatgct
      121 ttggtaataa agaaagaaca aggatttaca ggtacagaga ccaaaagctt attcttaaaa
      181 gataaaaaag ataataacta cgtatatctg acttttacta cgaaacaaac ggatttcaaa
      241 gcattaggga agatcgtcgg taaaaggctt tctattgttt catcagaacg catggaagaa
      301 caaacgggac aaaagcaggg cgctgtttct ccgtttggtt atgaaaaaca gattccgatt
      361 attattgatg aagagctctt atcaaatgaa aaacttgttt ttgcaccagg tagaccagat
      421 caaacgatgg tcatccaaac ggttgactta aacaaaatca tccagctact tgagatgccg
      481 atatttttga tgccggcaaa cgaaaaaaac aattaaccaa gtaaaatagt gcatacttgg
      541 aggtgaagca acatgataag ttatccatta ttagaaagaa atgcaacgat tggggttact
      601 gcaccatcct caggcgtatc agaggaacac cattcgttac tcgaatcagc tttaaagaga
      661 atggaagaaa aatctttcca agtagttact ggaaagacgc tttggacgca agataaggaa
      721 aaatcaggtc cagcaagaaa acgagcagat gaattcaatc aaatgatgca agatgaaaat
      781 atccaaatga tctttccgcc ttggggagga gaattattga ttgagattct cgattttgtc
      841 gattttgatg ctctacaacc aaaatggata gtaggttatt cagatattag tacgttgcta
      901 cttgctgtca cattaaaaac agggatagcc actgcacatg gaaccaacct tattgattta
      961 agaggagagt acttggatga aacgacagca atgtgggaaa gtgtactgaa aacaagagaa
     1021 ggagaatcga ttctacagca accttctgaa aagtatcaaa aagagtggcc ccatgagcat
     1081 tcatcatctt ctgttttcca tctcactgaa ccaacaaatt ggcaatcagt cagcggagat
     1141 gcagtaagga tgaagggacg attattaggt ggctgtattg atacgatcag tcatttaatc
     1201 ggtacccctt atggagatgt ccaatctttt aaaagaaagt ataatgatgg aaagccgatc
     1261 ttgtggtttc tagaaaattg tgagctatcg tcagttgatt taagaagatc acttgttcag
     1321 atgaaactgg caggatggtt tgatggcagc tcggggatca tgttcggtag aagtgcagca
     1381 gaggatttgt tagattataa aatgaaggat gtatatgaag atctgtcaaa ggaattaaaa
     1441 ctaccgatct tgtatgatat tgattgtggt catgtaccgc cacaactgac attgatcaat
     1501 ggtgcctatg cagaagtagc ggtagaagat agaaaagcga ctgttttgca aacgtttatt
     1561 taaaaaattt tttcctgcct tggagtttat caaagaccaa caaatctaaa tagaaaagaa
     1621 aatgaaagaa gctgacagta ttttgccagc ttctttttgt atgatctatt agaaatttta
     1681 attatccata actatcacat tgatcagctg tttcacatca tttggattac ctgtaggaat
     1741 agctactaca gatgcattat caataatttc ttgagttgtt tctctaatga acgctggata
     1801 agatgtttga tctgcaggca tttgccaatg tgaaggaact ggataactag tgattgtccc
     1861 gtcatggtta cttgaataca ctaaaactcc ctggtaactg tatttcccag tgagcataac
     1921 cacatctttc ggatagactg cactccctgc atcataaggg gcgataggtg ttcctgcagg
     1981 ataatgaata acatgtagtt cgaaatcacc cgatgtatct ctaaaagtat tacctctaat
     2041 tgctagccaa acacgtgcgt actcaatttg tagatcagaa tatcctgcta acggatctat
     2101 tgactgagaa gactgtgagg aagttgtgtc tattgttgaa gaagaaatat ttgtctcaac
     2161 tgtactacta gatgtagaag aagtattggg ctctgagact ttactgcttg ttgaagatgt
     2221 attcgtttca gtagaagctg atttctgagt acttgatttt tcagctgaac tggtttgttt
     2281 gattgaactt ttgtctgtgg tcgcagatgg atcactattt tctttactag gagacgtaca
     2341 tcctacaagg aataaagaga ccaacaataa aataaaacct aactttttca tataaaatac
     2401 ctcattattt ttttaatacc agtatagcat aatcctgaat ttttgaatcc gtaaaacctt
     2461 gtc
//
LOCUS       DACDVT010000161         2401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDVT010000161 DACDVT010000000
VERSION     DACDVT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.167"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..101)
                     /locus_tag="IWL52_002701"
     CDS             complement(<1..101)
                     /locus_tag="IWL52_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9779202.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQE"
     gene            complement(175..456)
                     /locus_tag="IWL52_002702"
                     /pseudo
     CDS             complement(175..456)
                     /locus_tag="IWL52_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            918..2299
                     /locus_tag="IWL52_002703"
                     /pseudo
     CDS             918..2299
                     /locus_tag="IWL52_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          872 a          413 c          402 g          714 t
ORIGIN      
        1 aattcctgaa cttcacgata gcttagattg taacgcaggt agtaaccaac agcgacaata
       61 atgacgtctt ttttgaattg tttgccttta aaatgattca ttactctgcc ctctctgtct
      121 tttttctcaa ttttacacta aaatagattt tttggaaaac tttgcaacag aaccgaaatg
      181 agtatagtat gaattagtaa tttggttttc atctaagttt agctttgatt tactaaaatt
      241 gatatctcga tacatgttca atacatctaa tacaaatcga gaatcttctt tcggaaatgg
      301 ctcagaaaga tcactccaaa tttcatcatc ataatgatac tcgtaacctt ctctcaatat
      361 ttcgattttt ctttcataat aagatctgtt ttggtcctca aatttagcca ataactcaaa
      421 ttgatttact agtgacaatc tctgaaaact gtccattaaa tgtttcttcc tatcttgttc
      481 attaacaaaa aattataagc ccttacaacg aatattatat attaaattta attgtaggtg
      541 aacacgaatt ttataaatat ttatggtgtt aatgacaaat gatatccgac tcctcttcat
      601 acgttctgga ctatttgcta atagattaaa atttcagcat ctatttctga ccataaataa
      661 atgagctgcc gaaaagctgc caaatcagac ttgaaaataa aggatcaaaa acttttaagt
      721 ttctgacccc gtaactcaca cttcaaaatc ttacttaaaa aggagtctga aagaaaaaaa
      781 gagtattcaa aatttttttc taactagcta catcacatct atgcattgac actcaaagaa
      841 ccagaataag tcatcagaaa gcaagggggt gcggacataa tcctggacga aaaaataaac
      901 ttaggaggaa tgtccgtgtg ttttcccaca aagagcgtgt aagggcaatt cagttatttt
      961 taaaatacga ttgctcttat gctgcaacca ttcgagaatt agggtatccg tcgattggtg
     1021 cactacggaa atggtataaa gagtatttgg aatccggaga gctccatcag gagcaccgaa
     1081 aaaaatcaaa gtattctgaa gaacaaaaac gaatagcggt gaatcattat tttgaatatg
     1141 gtcaatgtta tgcacgaacc attcgcatgc ttggttatcc taatagagag tccttgcggc
     1201 agcggtgtga agaacttgca ccaggagcta gaaaactccg aaaaagtgcg gtaaagttaa
     1261 cacaagatca aaaagaagct gtgttgaaaa aattctataa accgcaaact aataggaaaa
     1321 atttagcaga atctgaagga attagtagag ttactctata tcaatggaaa aacacggttc
     1381 ttggaaagga ttttccattg cgtatgactt taaaaaatca ggaaaaacaa aaagagctac
     1441 ttttaaagga agtagaagac ttacaaaaac aagtacacca attacagctc gaaaaaacat
     1501 tactcgaagg agcggctgaa ctgctaaaaa aagaaaaagg cgtcaatctt ctttgtctat
     1561 caaatcaaga aaaaacgata ttgattgatg ccttacgaaa tcagttcacc ttgaaagagc
     1621 ttcttcaaca gctccagttg cctaaaagca gctattttta tcaaaaacaa gcacttgaga
     1681 aaccagataa gtattacaaa gaacgtcaat taattatcac gattttcaat cataacttct
     1741 gtgcgtatgg ttaccgtaga attcaccaag ctttaaaaaa tatgggtaaa aagctttctg
     1801 aaaaagttgt acgtagattg atgactgagg aaaatctatt tgttaaattt tctagacgaa
     1861 aaaagtattc ttcgtatgct ggggaaattt ctcctgcaca ccccaattta ttaaatcgga
     1921 attttaaagc aaagaagcca aatgaaaaat ggttaacaga tattactgaa tttagaattc
     1981 ccgcagggaa aatctactta tcaccacttg tcgattgtta cgatggtgct attgttagtt
     2041 ggactattgg aacaaagcca acttgcttca ctctatgtct aaaaagggtt attcaccaga
     2101 taattcagcg tgcgaaggtt tctttggacg attaaaaaac gagatgtttt atcaacgtga
     2161 ctggaagaat acaacaatca atcagtttat aaacaaattg gatgattata ttcattggta
     2221 taataatcaa cgaattaagt tatctttagg agggctaagc cctcttcaat atcggaagaa
     2281 gcaaagatat atttgttaaa aaggtccaaa aaaatatccg cacccccttt ttttgtaaaa
     2341 aactattgca caatacaagt ggcctacggc tatcgaaatt tccataactt tcgagcaaga
     2401 a
//
LOCUS       DACDVT010000162         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDVT010000162 DACDVT010000000
VERSION     DACDVT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.168"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            807..1007
                     /locus_tag="IWL52_002704"
                     /pseudo
     CDS             807..1007
                     /locus_tag="IWL52_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="IWL52_002705"
     CDS             1494..1673
                     /locus_tag="IWL52_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779203.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="IWL52_002706"
     CDS             complement(1719..2324)
                     /locus_tag="IWL52_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9779204.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          794 a          395 c          427 g          783 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta cgataaagtc cgtataatt
//
LOCUS       DACDVT010000163         2351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDVT010000163 DACDVT010000000
VERSION     DACDVT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.169"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWL52_002707"
     CDS             <1..544
                     /locus_tag="IWL52_002707"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9779205.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IWL52_002708"
     CDS             complement(1017..1589)
                     /locus_tag="IWL52_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9779206.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IWL52_002709"
     CDS             complement(1605..2210)
                     /locus_tag="IWL52_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9779207.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DACDVT010000164         2248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDVT010000164 DACDVT010000000
VERSION     DACDVT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.171"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..716
                     /locus_tag="IWL52_002710"
     CDS             24..716
                     /locus_tag="IWL52_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9779208.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
     gene            915..2210
                     /locus_tag="IWL52_002711"
     CDS             915..2210
                     /locus_tag="IWL52_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9779209.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          737 a          438 c          433 g          640 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt ttggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      841 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
      901 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
      961 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
     1021 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
     1081 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
     1141 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
     1201 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
     1261 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
     1321 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
     1381 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
     1441 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
     1501 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
     1561 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
     1621 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
     1681 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
     1741 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
     1801 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
     1861 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     1921 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     1981 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     2041 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
     2101 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
     2161 tcaaactacg aattatgatt cgtcatggca aaacattttt aaccaaataa acagaacaga
     2221 aagaggtttt cttctttctg ttctgtcc
//
LOCUS       DACDVT010000165         2236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDVT010000165 DACDVT010000000
VERSION     DACDVT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.172"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            121..507
                     /locus_tag="IWL52_002712"
     CDS             121..507
                     /locus_tag="IWL52_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9779210.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            605..796
                     /locus_tag="IWL52_002713"
     CDS             605..796
                     /locus_tag="IWL52_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9779211.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1310..1642
                     /locus_tag="IWL52_002714"
     CDS             1310..1642
                     /locus_tag="IWL52_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9779212.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1552..1755
                     /locus_tag="IWL52_002715"
     CDS             1552..1755
                     /locus_tag="IWL52_002715"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9779213.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1939..2094
                     /locus_tag="IWL52_002716"
     CDS             1939..2094
                     /locus_tag="IWL52_002716"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9779214.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          812 a          375 c          375 g          674 t
ORIGIN      
        1 cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat aattgcatta
       61 gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga ggtgtgcaat
      121 atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga aaaggtcatt
      181 ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca tgaacaactt
      241 gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca accgattaat
      301 gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct tcctttgttg
      361 aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttaataaaac tgataaagaa
      421 attggaaagg ttctgggtat tacaagacag ggtgcgacaa atttaaaaca tcgactttac
      481 aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg tatgcttctt
      541 tgctcgtcct cctccttccc aaaaaaataa atagattata agttcatggg aagaaggtaa
      601 atttatgaat actttaaaag aagatttcat tttagcaaag aatggaaatg aagaagcagt
      661 tgtggccatt ctcaaaagat ttagcgcttt ggtccataag caatcctgga gaactggaaa
      721 atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta tctcgaaatt
      781 cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag atagtttcaa
      841 atttcgaaca ataaatttga agccatcttc tttataatta taaagtatcg attttacagc
      901 gttctataat gattcataga atctaaaacg aatgatttta attattaacg ttttttaatt
      961 cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac tttatttacg
     1021 tacgaagtgg gcaggaggtc gaatccagaa ctgggccatg gtacttcaac gaggacttct
     1081 cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt tattcaacta
     1141 tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg gttgttaaaa
     1201 actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag ttgggctatg
     1261 atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat tgaaaaaaac
     1321 atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa aagtaactac
     1381 caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat gggtttcaga
     1441 atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac ctgataaagc
     1501 agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg agtgttctac
     1561 gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga tgaaggaatc
     1621 gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg cacaagagga
     1681 tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat ggtaaaaaaa
     1741 atgttgaatc gataatttga acagaaccaa attgatgctg tttgggtgac ggatattact
     1801 tacattccat gtaatgacgg tcgcctctat ttgtccacct atattgactt agcaactcgt
     1861 attctacgtt actatatggt ggattcacac atgaaaaaag agattgtcgt ccgtccatta
     1921 caaatttatt aaggagaatt gcctacggta attcattccg atagaggtag ccagcatcgg
     1981 tcttttattt atcaagagct gttgaccgac caccacatca cacattcaat gagccaaact
     2041 gggactcctg ttgacaatgc agtaattgaa gcgtttcatc gatcaatcaa atgagaatta
     2101 atcgactcca acaaatataa aagtaaaata gagatgaagg tgttaatcca agattattta
     2161 gaagactact acccaaacaa aagaatccat acaaaattta tgatgactcc ttgacaattt
     2221 gaagcgaaga aaataa
//
LOCUS       DACDVT010000166         2218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDVT010000166 DACDVT010000000
VERSION     DACDVT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.173"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>2218
                     /gene="esp"
                     /locus_tag="IWL52_002717"
     CDS             <1..>2218
                     /gene="esp"
                     /locus_tag="IWL52_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9779215.1"
                     /translation="SVLVGVGLVLGTTELVQAQDEISPSTPLETAISSVQVGDKVASG
                     NTFQENPGYTKNYNFSDLQFSPQELTGDTLKGNTIGFEVYGKHNIAASTKNWEIRLQL
                     DERLAKYVEKIQVDPKKGIGSSRRTFVRINDSLGRPTNIWKVNYIRASDGLFAGAETT
                     DTQTAPNGVITFEKSLDEIFKEIGIDNLKTDRLMYRIYLVSHQDDDKIVPGIDSTGYF
                     LTDSDDFYNSLDVSENNPDQFKHGSVSAKYEEPNTQTKDGSGSTGANGAIILDHKLTK
                     NYNFSYSASAKGTPWYANYKIDERLVPYVAGIQMHMVQADKVTYDVSFESGKKVADLA
                     IERRKDHENYGVGSITDNDLTKLIDFANASPRPVVIRYVLQLTKPLDEILEDMKATAQ
                     VEENKPFGEDFIFDSWLSDTNKKLIQNTYGTGYYYLQDIDGDGNPDDKEESGDTNPYI
                     GKPELEEVYDVDTTVKGKVFIHELAGTGHKAQLVDKEGTVLAEKTIAPNEKDGAPISD
                     TVEFEFTGVDSSKLIAKDELKIQIVSPGFDKPEEGSTVIKESPKAVDKQTVVVGFKPD
                     AKESIRNNKNLPEDAEYSWKTEPDTSNVTDSTKGIVTVKIGNRTFDVDVEFAVKASQA
                     MENDATYVPITTTPETTVQSGKPTFDKPDVPLAKDAFSILDVYNKDFGNASVDANTGI
                     VTFTPAKGVGESEPITGTIPIKIVYQDGSVGTTDLAVTVSKDIYENPGENIPAGYH"
BASE COUNT          795 a          353 c          451 g          619 t
ORIGIN      
        1 catctgtttt ggttggtgtt ggtcttgtct tgggaacaac tgaactagtt caagcacaag
       61 atgaaatttc tccaagcacc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca
      121 aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt
      181 cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg
      241 gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc
      301 ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag
      361 gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa
      421 atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg gctgaaacaa
      481 ctgatacaca aactgctcct aatggggtaa ttacgtttga aaagagctta gatgaaattt
      541 ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg
      601 taagtcatca agacgatgat aaaattgtac caggtattga tagtacgggc tactttttaa
      661 cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta
      721 aacatggatc agtaagtgca aaatatgagg aacctaatac tcaaacaaaa gatggttcag
      781 gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt
      841 tctcatattc agcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac
      901 gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat
      961 atgatgtttc ttttgaatca gggaaaaaag tggctgattt agcaattgaa cggcgcaaag
     1021 atcatgaaaa ttatggtgta ggttcaatta ctgataatga tcttaccaaa cttattgatt
     1081 ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat
     1141 tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg
     1201 gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca
     1261 cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata
     1321 aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg
     1381 atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca
     1441 aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg
     1501 aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa
     1561 gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac
     1621 cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg
     1681 tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag
     1741 atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag
     1801 gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa
     1861 aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa
     1921 cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg
     1981 ccttttcaat tttagatgtc tataataagg acttcggtaa tgcaagtgtt gatgcaaata
     2041 ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa
     2101 caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa
     2161 ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaa
//
LOCUS       DACDVT010000167         2214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDVT010000167 DACDVT010000000
VERSION     DACDVT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.174"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(117..707)
                     /locus_tag="IWL52_002718"
     CDS             complement(117..707)
                     /locus_tag="IWL52_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9779216.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IWL52_002719"
     CDS             1230..1391
                     /locus_tag="IWL52_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779217.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IWL52_002720"
     CDS             1410..1904
                     /locus_tag="IWL52_002720"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP9779218.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          795 a          340 c          375 g          704 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaaaacacag ctaaaaatta
     2161 gttgtgtttt ttattaggtt tacttttctg gtataaaagg aactagctac tttt
//
LOCUS       DACDVT010000168         2145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDVT010000168 DACDVT010000000
VERSION     DACDVT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.175"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(289..606)
                     /locus_tag="IWL52_002721"
     CDS             complement(289..606)
                     /locus_tag="IWL52_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9779219.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYEMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(607..861)
                     /locus_tag="IWL52_002722"
     CDS             complement(607..861)
                     /locus_tag="IWL52_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9779220.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1121..1339)
                     /locus_tag="IWL52_002723"
                     /pseudo
     CDS             complement(1121..1339)
                     /locus_tag="IWL52_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          685 a          398 c          325 g          737 t
ORIGIN      
        1 tctcatgagt aaagtcacga aatttttggg aatagagcgt acttgatatc ttattttttc
       61 actaatagca gtgtctctta ttcaccaagc gatccccgta gttcctacgg ttcttaattt
      121 ttctctagac taatgattgt atctttagac cttcggccat aacatggagc aaaataaaaa
      181 ggtactaagt cttatatatc aatcttcgag taaccgatgg ttaacaacca tatcgagcgg
      241 cattaatcac ccacctgtag aggatgagcg acttttgcct aattattgtt aaaagcctag
      301 tttcatttgt ttagattgga aaatacgtcc tacaagtatc tctttttttt catggtcgat
      361 ccgataaaat aaggcatagc ttttgccgat cacgatccga tatgttggga tatccatttc
      421 atagatttcg gaaacttcct catgcatagt tggaaaattt ttggttagtt ctaaagcctt
      481 ctggatcgca cctacaaatc tttttatgct ggctggagaa agagcaagct cattttccca
      541 ttcttctatt atcgtttcaa ggctggtctg gaaactttta gaataaacta gcgtgtactc
      601 tttcatctat tcactccagc cgtattttcc gaaagctttt accatttcaa tttctgaaac
      661 tgttaaacct ttctcaaagt cttggaaact ttcttcaatc aatttgcgat cgtgttcttt
      721 tgctatttga gtagtatcta atatgaataa gttgccttct tgtttggctt ggatagtgtc
      781 tccgcttttt aatccaagag agcttggaag gatcactccc acgctattgc cgatctttct
      841 aacctttagt tcttttccca tgttatcaca ctccttttag tttttgataa ataaattata
      901 acatgttata acaacaattg ccaacggtgt ttcttaaggg attccactcc atattattat
      961 tggacaaaat aagccactta gaacgctgaa aacatataca aacgatatca aaatacgagt
     1021 gaactactca ccatacactt ccgttaacat ccatttaagt aggtattttt attatatata
     1081 gatttaacat atgtcggtcg gttgattgat atagctaaag ttataactca accgaagccg
     1141 atttatctac acgaataaag ttgcgagtgt tctcggctaa ttcataaaaa ttgttatgtg
     1201 ttccatatag caatactgga ataactttat tttcttgaat ctcagaagca actctataca
     1261 cggtgaccgc atacttaaat ccattagacc aattacctga taaacctcca gttttttgct
     1321 cttacttaaa tggatcaata gaaatttcat cacaaaccta tagaggatga gtgacttctg
     1381 cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag caaaaaatat ctagaatttc
     1441 atttcctcaa aactgatcga tattgtatcc aaaaatatat actacctaaa ataggtagta
     1501 tatattaaaa tatgtgcttt tgttagaaaa atcaatacat aaattatcat atctgtatat
     1561 gtcatttagg tattttagtt atgttataat agttttgctg tctgctaccc cggcctaatc
     1621 tggcaagggg aggaggagat ataatgaaat acctttttga ttttgttttt gcaatcaagc
     1681 catttgcaga ggtaactgca cactacatct gcaagtggct tgattgcaaa attgccaaaa
     1741 aagacagtaa gcctacccgt taattctata atatagaaac gtaaaaaaaa accccatcag
     1801 cctgcacgct gatggggttt ggtgatataa tgaaatacct ctttgttact cataattata
     1861 ccataattag tcacgaatgc aaccaataga aaaaactact tagaacggta gatcatcgct
     1921 aataacaatg cattgtccac caaatggatt ttgtgaagca ttaaatacat tgttattaat
     1981 tgtcgctgct tttaactagg cctctcccct tctattataa aagaagatat atatcatttc
     2041 ttaattacct actaccttaa accaaaattt aagtactacc taatttaagt agtacttaaa
     2101 taatctaaaa aatagttatc ccaattgtta tgattttata atacg
//
LOCUS       DACDVT010000169         2142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDVT010000169 DACDVT010000000
VERSION     DACDVT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.176"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(493..1785)
                     /locus_tag="IWL52_002724"
     CDS             complement(493..1785)
                     /locus_tag="IWL52_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9779221.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          692 a          409 c          341 g          700 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta tt
//
LOCUS       DACDVT010000170         2138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDVT010000170 DACDVT010000000
VERSION     DACDVT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.177"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..497
                     /locus_tag="IWL52_002725"
     CDS             168..497
                     /locus_tag="IWL52_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9779222.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            487..774
                     /locus_tag="IWL52_002726"
     CDS             487..774
                     /locus_tag="IWL52_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9779223.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1053..1616)
                     /locus_tag="IWL52_002727"
     CDS             complement(1053..1616)
                     /locus_tag="IWL52_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9779224.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          765 a          322 c          337 g          714 t
ORIGIN      
        1 ttgattccaa gttaaaaaca tattattatt ctacataatt tcctaatcaa ataaaactcc
       61 tttgaaaatt gttctaaagg agttttatta ttttgtattt ctactaaaaa tagcgtacaa
      121 tgtaattact aagtaattac gaaatgtaaa ataatggagg aaataatatg attacaaaaa
      181 caagaaaaca aggaaattct attatgttaa cggtccctaa agaattcgat gtgcccaatg
      241 gtgttgaagt agaagctaag cttgtagaga atggtatcct ttatgagttt gtagagccta
      301 aaaaagaatt ttttgatttt agtgaagata ttttggctga tattctttca gaaggttata
      361 gcaaacaaga gatcttaaaa gaatttagaa atagaaaaaa tgcgttaact tctgcattta
      421 aatctattgc tgaagatact gttgcaaatt ctaaaccgat gacaaaagag gagttggcag
      481 cagaaattgg cttatgagat tctaccttct aaacacgtaa taaagtatct aaaaaaatta
      541 aaagagaaaa cattaaaaga acaattttta acgataattt atgatgagat agcagttcgt
      601 cctcattcgg gagaacaaaa aacgggtgat ttatcaggaa tttgggctat tggattcaag
      661 tatgcaggta cgacatatcg cgttgcatat gaaattaaag ataatgcagt gatacctaac
      721 ttactatgtg gaacgcatga aaatttttat gaacaattaa aaaagattag gtaagaaccc
      781 aatgtttcaa cttgagagga tagacgacac tttaaaagat aatttttaac agctgagagt
      841 tgtaataccc tataatttta aaccactaac ctatatattt atgaaaaata gaaaaaatta
      901 aaacgcattt taagcctctt tcaaattaaa agcttcatcc ttatgcacaa agagggctta
      961 aaatgcgtta ttttatattg ttttttagta ataataaaaa aatcactcta attctcccat
     1021 aaatattata gattcctaaa gattaaaata gattattccc tattgtcttt taagtagcgg
     1081 tagagtgttg ccttgctaat accagtcatt tccgtaattt cttttacgct ataaacctga
     1141 cttttataca attttactgc tcgatcaaca tctttctggt tcactcgtgg acggccacct
     1201 ttacgacctc tcgcacgtgc acttttcaaa ccttctttgg tacgttcaac aattaaatca
     1261 cgttcgaact gactaaatgc ttggaagaca gtcatcatta agcgtccgtg aggcgtgctg
     1321 gtatcgaagt tttctttcaa gctcaccagc ttaacatggt gttcttcaaa ataggtaaca
     1381 aggtcaatca aatcctttgt actacgaccc aatcgagaaa aactttctac aattaccgta
     1441 tctcccggac gaattttatc ttttaaacga tttagttggg gacgaaccgc tttggttccc
     1501 gtcattttct cagttaggat ttcattacaa ttttgttcat tgagtaagtc taattgacga
     1561 tttagttctt gtgaacgtgt actcactcgg gcataaccat aaatgtatcc actcatgctt
     1621 ttcttccttt cgtactttaa ttctggcttg attgtatcat aaaagggagt tatagataca
     1681 tagttaatgc aacaagttat gatacaaaat atgacctctt ttatttaata aaagaggtca
     1741 tttcattttg tctcataatc gattgttttt gatacgagtg tgtatacgaa ataaacaaaa
     1801 aaatcttaag taaatttcta gatacctaat ttaatcctaa ctagtgtatc ctaaagttaa
     1861 taacgagttt atcacaatga aaattataaa atgctcccat attagatata acatgcaata
     1921 tttgaattag aactacaaaa tttattcgtt ttataaagta aataatgttg agatactaag
     1981 gaatagaata ttataaggaa ttgagctgaa tgtattggat aaaaaaagaa taactttatt
     2041 agagatggac tataaaaatg ctcgtaattt tcttctgaag tctgaaagct atttgaatgc
     2101 tcagctacca gaatatatta gttttgacga aactttaa
//
LOCUS       DACDVT010000171         2013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDVT010000171 DACDVT010000000
VERSION     DACDVT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.178"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="IWL52_002728"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="IWL52_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9779225.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="IWL52_002729"
     CDS             851..1039
                     /locus_tag="IWL52_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9779226.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="IWL52_002730"
     CDS             1052..1609
                     /locus_tag="IWL52_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9779227.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="IWL52_002731"
     CDS             1641..1883
                     /locus_tag="IWL52_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9779228.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          736 a          309 c          407 g          561 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     1981 tctttattcc ttatgaaaaa atatctttaa aaa
//
LOCUS       DACDVT010000172         1944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDVT010000172 DACDVT010000000
VERSION     DACDVT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.180"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWL52_002732"
     CDS             <1..153
                     /locus_tag="IWL52_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9779229.1"
                     /translation="EENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVL
                     KIAEEK"
     gene            154..906
                     /locus_tag="IWL52_002733"
     CDS             154..906
                     /locus_tag="IWL52_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9779230.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            911..1858
                     /locus_tag="IWL52_002734"
     CDS             911..1858
                     /locus_tag="IWL52_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9779231.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          678 a          263 c          389 g          614 t
ORIGIN      
        1 gaagaaaaca ttgttaaaga aatgcctgaa tctgctaatg atatgtatga gaaggagttg
       61 aaatatttct ttgaaaacgt agtaacaggc aaagaaaact ggaatgattt atatcatgca
      121 aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa ttttatttac tatttgtggt
      181 agagctgggt ctaaggggtt taaaaataaa aatttgaaat atttggcagg aaagccccta
      241 gtccatttcg ctttagcaat gatagatatc tatcaacaaa aacatcctga acataattgc
      301 acgacttgtt tgaatacgga cagtaatgag ttagtagaat tgacgactaa gttaaatccg
      361 tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag atactgtagg gaaaattgaa
      421 gtgatacgtg attcattctt acaactagaa agagaagata atatatttga tttaattata
      481 gatttagatt tgacttcacc tttacgtgaa gtttctaatc ttgaagaatt aatagatgaa
      541 catctaaaaa atttagataa agacgtctac ttttcagttg tttctgcaag acgtaatccc
      601 tattttaata tggtaaaaag aattgaaaat gaagttacta tagttaataa atcaagatac
      661 acatctaggc aacagacacc tgaagttttt gaaatgaatg cctcaatgta ttcttttaaa
      721 cctgaatttc taaaaaaaag taattatatt tttgatgggc aatgtgggat aataaaaatg
      781 agagattatc taattttaga tattgatagt gaagaagatt ttaaatggtt agaatttttg
      841 tttcctaaat ttttagaaga ttcttcggag cttcaagata tctataatta tttatatcgg
      901 aggtaaaagg atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt
      961 agccaatcat ttaggagaag aacatgtggt aatcgtcgtc gatgacttat caatgggaaa
     1021 agtagaaaat cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa
     1081 atccttgatg gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc
     1141 agttgctagt gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga
     1201 aagtgtatta atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt
     1261 gtttagttct tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc
     1321 cgtgattcgc ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct
     1381 agattattgt catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg
     1441 tccaaatcaa aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta
     1501 taagaaacaa atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc
     1561 tagagacttt gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga
     1621 agctgcactg gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt
     1681 gattcatgca atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg
     1741 ttcgggtgat atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca
     1801 accgaaatat ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa
     1861 catactaaga taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt
     1921 ttgtaaaatc aaatctggga taga
//
LOCUS       DACDVT010000173         1936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDVT010000173 DACDVT010000000
VERSION     DACDVT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.181"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..532
                     /locus_tag="IWL52_002735"
     CDS             134..532
                     /locus_tag="IWL52_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9779232.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            516..1211
                     /locus_tag="IWL52_002736"
     CDS             516..1211
                     /locus_tag="IWL52_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9779233.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1805..>1936)
                     /locus_tag="IWL52_002737"
     CDS             complement(1805..>1936)
                     /locus_tag="IWL52_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9779234.1"
                     /translation="SELEKIATEAKTLDTEFMDKTIRKVMKKDNVTIKDPYVKNIFK"
BASE COUNT          717 a          282 c          365 g          572 t
ORIGIN      
        1 cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat gtgttattca
       61 ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg
      121 aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta tagaagcaga
      181 agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg aaacattaac
      241 agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt gtgatatgaa
      301 gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg tccaaactaa
      361 tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc cggaacttcc
      421 aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga caatcattaa
      481 aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt gatacagcaa
      541 atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta acaacaaatc
      601 ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa gaaatcaaaa
      661 aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa gaagaaatga
      721 tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc aaggtgccgg
      781 tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat ttgattaccg
      841 gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga gcagattata
      901 ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa gtgattgatt
      961 cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca gctagtttca
     1021 aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata acgttaggta
     1081 aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc
     1141 aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg
     1201 attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag
     1261 gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac taactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt
     1681 tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa
     1741 taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat
     1801 ttgtttattt gaatatattt tttacataag gatctttaat agttacattg tcttttttca
     1861 tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa
     1921 ttttttctag ctcaga
//
LOCUS       DACDVT010000174         1924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDVT010000174 DACDVT010000000
VERSION     DACDVT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.182"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            32..703
                     /locus_tag="IWL52_002738"
     CDS             32..703
                     /locus_tag="IWL52_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9779235.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPID
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLTLPIFK
                     GANQYTLSLGAATYFYEDAEMRKGNYVLAGHNMEIPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            756..>1924
                     /locus_tag="IWL52_002739"
     CDS             756..>1924
                     /locus_tag="IWL52_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9779236.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDVDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQAL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNNS
                     KGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELT
                     KFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVE
                     VPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVID
                     KTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWG"
BASE COUNT          678 a          298 c          432 g          516 t
ORIGIN      
        1 ttttttgttt tgtctgaaag gaggcagatg gatgaaaaaa tggttatttg gttttttagg
       61 agtcgcttta atcgtctttt gcagtgtgtt tggctatgtt agctaccaaa aacatgaagg
      121 agaggtgttt aagcaaaata tagaaaaaaa gatgcccata gatcaaatca atgaacatgc
      181 aaagagttat aaggaggatg caacgaacgt taataatgat atgtctttgg gacaaatgtt
      241 gtccatacaa aaagaagcaa tcgaaatggg cgtcaataaa caagcctttg cacaaattca
      301 gattcctgcg cttggtttaa cattgccgat ttttaaaggt gcaaatcaat acacattgag
      361 tcttggtgcg gctacttatt tttatgagga tgccgaaatg cgcaaaggaa actatgttct
      421 agcaggtcat aacatggaaa ttccaggtgt tttattttct gatattcaaa agttgtcact
      481 aggggaggtg atggacttag ttagtaacga tggcgtctat cgttacaagg taacgcgtaa
      541 atttatcgta cctgagtact ttaaattaat cgatggcgtt cctgaagaga actctttctt
      601 gtctttacca aagaaaggag agaaaccctt attaacgttg tttacttgtg tttatacgtc
      661 acaaggaaaa gaaagatacg tggtacaggg agagctacaa taacaaggtt ttaatcaacg
      721 tgagtcgttg aaatatgaaa cgaggagagt aaaaaatggc aataaatcgg tatgtaaaaa
      781 aagctggaat gcttgttgga atgttaggaa tcattgcagg ctgctttcag ggatatcgag
      841 catatgcaga agatgtcacg caaaaaacgc caccagagaa ggtcaatatc acggttcata
      901 aattaatgta tgatcaagga acgcaattga atgttgatgt tgacgggatc aaaaacgatg
      961 gttatacgca cgatcaatat cctacgggtg taacgaaata caacaaggca gattatggtg
     1021 atgttgagtt tacgttagga aatattacgg atcaagcatt accgagggaa gattctgatc
     1081 taaccaacgc gaaagtggac gaaatcgtta aagatgtaga agacaaggga tctaacagtg
     1141 agtacgtcaa gaatgcaacc aacaaaatta cttcagctgt agatgaaaat ggtgagatta
     1201 cttttgctga tcaaccagcc tatgtcaaca attccaaagg gaatgtatat gtggtttatg
     1261 aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc
     1321 caatgactga taatacaggt tcaggttttt taaaagacat tcacttatat cctaaaaata
     1381 ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcaca gctcaaagca
     1441 aacaaacacc tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa
     1501 caaaattagg agatcttgta tcggatgatc aaggaaagct gactgctact gatttgacgt
     1561 taggaaaata ctattttgtg gaagttcctt cagaagttgt agtgggatca gataaagaac
     1621 caacagccga tcaatattta ttaggcgcag acgctcgcaa cgatgcgcac aataaattaa
     1681 cctttgaaat tacgaatgat ggcgtgacaa gtgacttaaa agcttcatac gtaaactata
     1741 aagcaccagt gatcgataaa acagtaacaa atggaacagg acaagaacat tctttccaaa
     1801 ttggtgacgc agtaaattat caaggaacga ttcatatccc aacagacatt gcaggcggag
     1861 cagacggtat cacagtaaac ggagtgaaaa gcgaaacttc tccttacagt gtatttaaat
     1921 gggg
//
LOCUS       DACDVT010000175         1920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDVT010000175 DACDVT010000000
VERSION     DACDVT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.183"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            672..1802
                     /locus_tag="IWL52_002740"
     CDS             672..1802
                     /locus_tag="IWL52_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9779237.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          579 a          308 c          389 g          644 t
ORIGIN      
        1 tgcaagggca gtacttagaa gttccgccat tgcaagacca ttttgtcgtt actactgtta
       61 ctttagatgg taaggaaata gaaatgccag atcaaacgat tagcggattg tttaattact
      121 ttaacaaata aatatttatt ccgaaaagtg aaaaaacata taaaaaaaca cataaaacta
      181 aaaaattttc aaagaatcct ggtgcaactg aaagaatttg caacgggatt ctttcaactt
      241 atttgttatt tttttctaaa aaatattaaa gattgttaaa tttcactatt tcatttcaaa
      301 ttccattgcc taatcattag gaaaagtcta tcatgaactc gtaaatagaa tatagtaata
      361 accttgttag gtgaggctcc tatacaaaca taggctgttg cccagaaacg tcgaaagtcg
      421 ccaatgggta aaacagaaac tgttggatta aggctttttc caatagctaa gaatgttttc
      481 tttacgttgt atagtgctaa aactcggacg atgagatgat gctaggtcag aattcttgtt
      541 cgaaaaggtt tctgaccttt tctttatatt tttgtgactg acttatttga tgagacaaac
      601 tcgctcctca cctgacaagt ggattgctgt gtgaggagga gatgcggtga aaaaagatcg
      661 attggtggat tttgggtcct tatttgactt tgtccatgat cggtctttta gaagtataca
      721 gtgccagttc ttatcgttta ttgcaagccg atgaaaatac aaaaagtcta ttattgcgtc
      781 aactaatttt catatttttg agttggagcg tgatcttctt agctcgttca gtcaaactac
      841 actatttact tcaccctaaa atagcaggat acggtttagc cttatcgatt ttctttttag
      901 tattagtaag aatagggata ttcggtgtca ctgtcaacgg cgcacaacgt tggatctctc
      961 tgtttggcat tcaattccag ccttctgaac tggcaaatct ttttttgatt ttttatttaa
     1021 gctggttttt tcgtgacgga aatagtagcc caaaagatct aaaaaaacca ttcctgatta
     1081 cagtaggtat aacttttctg attttatttc agccaaagat tgctggagca ttgatgatcc
     1141 tttcgattgc gtgggtcata ttttgggcag cggcggttcc atttaaaaaa gggatctatc
     1201 taatcgttac tttttctgca ttgctgattg gagcagcagg cggggtatta tatttaggaa
     1261 ataaaggttg gcttccacag atgtttaatc atgcttatga aaggatcgcg actttaagag
     1321 attcttttat cgatagtcat ggtgcgggct atcaaatgac ccattcgttt tatgcactat
     1381 ataatggtgg catatttggg cggggattag gaaatagtat cacaaaaaaa gggtatcttc
     1441 cagagacaga aacagatttt attttttcga ttattacaga agagcttggg ctaatcggtg
     1501 cattgtgtgt gcttttctta ttgttctcat tatgcatgag aatcttttgt ttaagcagtc
     1561 gctgtaaaaa tcagcaagct ggattgtttc ttttaggatt cggaactttg ctattcgttc
     1621 agacgataat gaacgtggga agtatcgcgg ggctgatgcc aatgactggt gtgccattgc
     1681 catttgttag ctacggagga acaagttatc tgatcctttc tttaggaatc ggaatcacgt
     1741 tgaatatttc ttcaaagata caggccgaag agttggcgct ctatcgacct gaaaaacaat
     1801 aaagagaaac tgagaaactc tcagtttgaa ttatcataaa gtacaatctt ttatttttca
     1861 agcgagaggt tcttttagaa agaggaattc gcaatgaaag tatctcaaaa aatcatatta
//
LOCUS       DACDVT010000176         1900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDVT010000176 DACDVT010000000
VERSION     DACDVT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.184"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            173..628
                     /locus_tag="IWL52_002741"
     CDS             173..628
                     /locus_tag="IWL52_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9779238.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            642..>1900
                     /locus_tag="IWL52_002742"
     CDS             642..>1900
                     /locus_tag="IWL52_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9779239.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGV"
BASE COUNT          594 a          261 c          398 g          647 t
ORIGIN      
        1 aagtcaaatt agtcattatg gttagtttag ctgaagaaga tgtatctgag attagaggag
       61 tatatgaaga actttaccaa tttattgggc aaaaagaaat ggtcgatcgc ttgttacaag
      121 ttcatgatag cgaaaaatta ctaagaattt ttcaacaaaa aggagaataa aaatgtatct
      181 agataaagaa ttatgtttat ttcaattaga agtgaaagat caaaaagaat tgtttcaagt
      241 aatgtctgaa caattaaaaa acgctggttg cgtgaaagat agtttcttag aaggtatcac
      301 caaccgtgaa caagaatttc caactggatt agaagtaaat caaattgggt ttgctatccc
      361 acatacagat tccgcacatg taaatagctc tcaaatctgt tttgcctcat taaaggaacc
      421 actggtattt tcagatatga cagataaatc tcatgaaata cctgtacgtt tagtctttat
      481 gttggcaatg tcacaacctc atgaacaaat tgatacacta caaaacttag ttagcctatt
      541 tcaaaatgaa gaaaaagtaa atgaattact tgcatgtacc acgaaagaag catttattga
      601 gttagtacat tctgcaggag tttattagga ggaatagaga gatggatatt gtattaaatg
      661 cctttgaatg gtttattgga ttagggtcaa acgtcttttt accactgatt atttttgtaa
      721 tcgggatatt atttggatta aaacctggta aagcatttgt ttccggcgtt actgttggta
      781 tcggttctat tggtttagga ttagttttaa gtttattatc agatggttta ggaacagcaa
      841 ttcaacaaat gggggaacaa tacggtggtg gcctgaatat cctagatatt ggcgtaggtg
      901 ttggtggacc tttggcttac tctacatcat tgggaatttt aatgattcca atttcattaa
      961 ttcttaactt catatttgtg gttttgggtt ggacaaaaac attgaatgtt gatatttgga
     1021 acttttggtt cgcaattttc ctaggtatga ttactcaatc tgttacggga aacttttggt
     1081 ttggcttaat tggcgcgatt gtcgcagtaa ttttacaatg gttcttagca gatcgtacac
     1141 aaaaaactgt tagcgaattt ttcggctatc caggaatctc tatcacacat atgatgggat
     1201 tatcaggagt actatttgcc aaaccagtaa attgggtttt tgatcgtatt cctggattta
     1261 ataaaataga tgcagatgca gaaacattaa caaaacgttt cggtgtttta ggtgacacag
     1321 ttgttattgg cctaattatc ggtattgtag tgggtattat ggcaggctat gacgtagcag
     1381 gaattggaaa cttaggaatt atgactgcgg cagtgatgaa gattatgcct aaaatggtag
     1441 caatgtttat ggaaggttta atgccaattg cagaagcggc gaaagaattt acgaataaaa
     1501 agttaaatgg tcgccaagta aacattggaa tggatgcagc gttaactgtg ggtcatccaa
     1561 ctattatgtc aacttctcta ttgcttgtac caatctcatt attgctagca ctaattcttc
     1621 caggaaataa agtattacct tttggagatt tagcattctt tgctttcgcc atctgtttaa
     1681 tgattcctta ttttaaagga aatattgtga gatcattagt aggatgttcg ttgtatgttg
     1741 ttacaacatt atatatggca acatggttgg caccaattat cactgatgtg tttacattag
     1801 caagtttcga tgtggggaca gcaggattag taactatgtt gttatgtgga ttatggccag
     1861 tagggttgtt tgttttatta gcatcttact ttggtgtaat
//
LOCUS       DACDVT010000177         1766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDVT010000177 DACDVT010000000
VERSION     DACDVT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.185"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWL52_002743"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWL52_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9779240.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IWL52_002744"
     CDS             complement(1305..1709)
                     /locus_tag="IWL52_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9779241.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          528 a          303 c          280 g          655 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaagg
//
LOCUS       DACDVT010000178         1736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDVT010000178 DACDVT010000000
VERSION     DACDVT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.186"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(74..1072)
                     /locus_tag="IWL52_002745"
     CDS             complement(74..1072)
                     /locus_tag="IWL52_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9779242.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1147..1443)
                     /locus_tag="IWL52_002746"
     CDS             complement(1147..1443)
                     /locus_tag="IWL52_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9779243.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1436..1729)
                     /locus_tag="IWL52_002747"
     CDS             complement(1436..1729)
                     /locus_tag="IWL52_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779244.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
BASE COUNT          499 a          337 c          233 g          667 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cgaaattgtt ttccaccagc catggtcgtg tttccatcat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatacatt tttatcagga gaatcataca
      301 accataaagc tttattattt agcacagatt ctttagcagc ttcccaactt gaaattcctg
      361 aactacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gtgtttgagg agtttgtcca ttcacaatat cactgaaacc ttgttttaac tgttgatttt
      481 gagtttcgtt aaagtacaga taattatttt cattaccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aaaccgaaaa agtaaacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatcgcca ttaatcacgg
      721 cttgtggtaa aggagtgcca tacagactta cattattctg aagattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtgtga caaaagtttc taatttggaa gctttattcg
      841 tatcccacaa agtattctta acctcttcag aagaagattc agtgattgaa cttgcacttg
      901 tttccacaga ttgcttcatt tctgttgtag tttgttcagt tgattgcgtt ttactttttt
      961 tagtctcaat tgttgtatca gtttctatag tcgctttagt cctcgtattc tcagatgtac
     1021 aaccagtgaa taaaattccc attcctatta gtaataacag ccattttttc attatatttc
     1081 cccctttcaa tttctccctc acgtacttat tctatcagac aattaattga tctaaatggt
     1141 aatcttttaa tcgattcttt cccaataatg tcgatagccc gtatggcagc ggtctcttcc
     1201 ttgaggactc tcagatggat taaaactagt agaaatttcc cttccacatc ctctacagcg
     1261 ataagttctc attatttttg ggctattagt attatttttc ttagttagag ctatgagtcc
     1321 aactatgatt accccaactg tcttaattac ttttccttta tctatattcg gatgcgcaag
     1381 catatattta tataaccaat atccaccgta aattaaacca cctaatacaa tcagcttagc
     1441 catgtactcg tctcctttct ttttgtttaa ttactttttg aatccatctt gcagagaaac
     1501 cataaacttc tgtcaattga cggatatcta cagttttttc ctgttctatc atgttttcga
     1561 ttctttttct aactaactca cgatcgtaca ttttcattgg aaaagaaatc tgttgccctc
     1621 gaaaagcaag ataaaattga tagagcaatt ctgaatccag ttccatttcc tcaaaaaagt
     1681 cattcatact tttataagag ttactcaaac aagtttcatc aatctccatc tacaca
//
LOCUS       DACDVT010000179         1729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDVT010000179 DACDVT010000000
VERSION     DACDVT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.187"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWL52_002748"
     CDS             949..1422
                     /locus_tag="IWL52_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9779245.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWL52_002749"
     CDS             1431..1658
                     /locus_tag="IWL52_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9779246.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          582 a          269 c          258 g          620 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattt
//
LOCUS       DACDVT010000180         1717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDVT010000180 DACDVT010000000
VERSION     DACDVT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.188"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..921
                     /locus_tag="IWL52_002750"
     CDS             64..921
                     /locus_tag="IWL52_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9779247.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            922..1470
                     /locus_tag="IWL52_002751"
     CDS             922..1470
                     /locus_tag="IWL52_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779248.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          653 a          232 c          359 g          473 t
ORIGIN      
        1 actaaaaata aatatgttat aatagtatca aatgaatatt gctaaaagaa aagaggtagg
       61 aacgtggtag cgaagaaata tgtaatcggt aattttaaag gtggcgtagg taaaagtacg
      121 tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag gaaaaagaac cttgataatt
      181 gacttggata tgcaaggaaa tacttctgat gtgatgaact taacgcatat gaacttcact
      241 aacgaagaag gaggcggaga aggtgaaccg attgtttacg aaaatactgt gactgacgtg
      301 ttgataaaca atcttccacc taaagaggct gtctataaag taatcaataa cctctatata
      361 cttccagcag acatgtcctt tgaaatgtat gatgattgga tcaaagacca atataatgat
      421 tcattatccc aattcaaata tatgaaagaa aagctagaac cattatttga tgagtttgac
      481 gtcatctatt tagatgtccc accttctatt tctgtatata gtaaatccgc gatgtacatt
      541 gcggactggg caatcgtcgt cttacagacc caagtaaaat cgatgagaaa tgcgatgcaa
      601 tatcttgagt atatggactt ttttgtgaaa gaatttgata caaatttata tgttgctggt
      661 gttattcctt tcatgttgga aagcggtgac gcagtagatc aggaaatgta taaacaagcc
      721 caagagattt atggggaaca tttgattaaa aatgttgtac tcaaaaatgc gcgcttaaaa
      781 cgatacgacg gatcaggtat tacgatggag aagacaaaga aaggtctctt aaaacaatgg
      841 gatcgcaaaa gccacgaatt atttatcagt attttgaaag aattggaaga acacgagaat
      901 tggtatgcgg aggtggagta attggctgac atcaaaggac ttgatatttt tgggaacaat
      961 aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca agcaattaca
     1021 ccatctgaaa ataagcaatt agaagtgcac caagttcaag aaataaaaac ttcctccaat
     1081 gttgaaagac agagtagagg aagaacaaaa aaagttggcg caccaatcag aaagtttgat
     1141 aaggtttatt ccaccaagca tcctttaaaa ctgtcgactt tgttaaatgc gacttccaga
     1201 gttttagtag aaaagtatga gactaatatg acaagagatg aattgcttag aaaagcttta
     1261 gatgagtata ttaaacagaa tcttacgcaa gaagataagc aagatttgtt taatgacgtg
     1321 gtgaaagaac tagacttgtt cagagaaaaa tatccaattg ttccagaatt agatgatcgt
     1381 ggagaaataa taagaactac cagtcagata aaacaagata ccgaagactc actgcgcaaa
     1441 gggtggggaa tgaataataa agttagataa taatcaaaga agaactaagt aactaccttt
     1501 aaacggtagt tacttagttc ttctttgatt tgcgtgaaat agaggggatt tatttttctg
     1561 gttacgtgat taaaaaatcc aaaaaatgag cataggaacg gttatgattt tagggtgtgt
     1621 tttaatgaga tattgtgatt tcattaacaa gtgtatgata atcggttctt ttgcaaagtt
     1681 ttccaaaaaa tctattttag tgtaaaattg agaaaaa
//
LOCUS       DACDVT010000181         1696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDVT010000181 DACDVT010000000
VERSION     DACDVT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.189"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            217..639
                     /locus_tag="IWL52_002752"
     CDS             217..639
                     /locus_tag="IWL52_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779249.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            649..852
                     /locus_tag="IWL52_002753"
     CDS             649..852
                     /locus_tag="IWL52_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779250.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1063..1647
                     /locus_tag="IWL52_002754"
                     /pseudo
     CDS             1063..1647
                     /locus_tag="IWL52_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          607 a          235 c          304 g          550 t
ORIGIN      
        1 accttatata gacaaaaact aatgatggag gaaatagtat gtgtcacttt tctttttaaa
       61 tggcaatatt ttttgtaaat aaatgtcaat caagaaaatt taaacgatta tgacattaaa
      121 aatatatttt atgaattttc tagtatataa cttgatttat ctgttaagat ttattaagaa
      181 aacgttttct tagttttgat ttcgaggtga tagacaatga aaaataagtt tattttatct
      241 ttaggtattt taactattgg aattagtgta actggaacta caattcctac atttgctgat
      301 agtacaaatg aaccaactac tgtagtagat aataaccaag aattaactcc tttagatagg
      361 gacggaatag ccattggtaa cccaactaac gacttaactg ttactgaaaa ttatttaata
      421 gtttctccat tacttgctct agcagcttct aaaacgatac gagtgacagg aacgatgatt
      481 tttgataaag ttggttggcc ttctagacct cctcaatcac ggaattggaa agaaaaaaga
      541 aatggttatt ggtactatgg aaaattgtat ttacaaagtt ataaatcagc tggaaacaat
      601 tggattgcga cttattcagg aaccttaact agaaagtagg gattttttat gccagaattt
      661 gtgaacacac caaattttta tcaagatagc gatattctaa gtaaaattaa tttaccgaaa
      721 gaagaatcag ctttacaagt tcagcgaact ttacaggaga cacaaaaaat tcaaaaatca
      781 atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa gagaccaaaa aacatatcaa
      841 aaagaaaaat aatgatttta aaaaaagagt gtctagtcga cattcttttt ttctaattga
      901 aagtgttcat aaaatcaccc atttctggac actttctttt tttgctttat ttttcaaaaa
      961 aacaattcat ttcatgttca taaacccata acaatatcta tgttcagtta aactatatgg
     1021 ataaagttat gttacaataa aattaatgaa ggagtgattt ttttgaaaat aggatacgcc
     1081 cgggtatcta ctggtttaca aaatttggat ttacaagaag acagcctaaa taaatttggt
     1141 tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa gtaatcgacc aggattagaa
     1201 atggccattg attttgttcg ctctggcgat acacttgtcg tttggcgctt agatcgttta
     1261 ggtagaaata tggaagattt gatttcaatc gtcaatcgac tgaatgagcg tggtgtgagt
     1321 tttcatagta ttcaagaaaa tattacgatg gacaaatcaa gttctacagg acagctcatg
     1381 tttcatttgt ttgcggcatt tgcataattt gagcgtaatt tgatcctgga acgatccgca
     1441 gcaggacgag cagctgctcg tgcaagagga cggtttggtg gtcgtcctga aaaattgtct
     1501 gaacaatatt tggaattact gaagacttta gtggatagcg gtacacctat taagactatt
     1561 gcggaacggt ggaacgtctc aagaaccacc atttatcgtt atttggcaaa aatggaggag
     1621 aaaaatggaa agacaaattc tcattagttt ttgtcattta atgctgaaaa tttttgccat
     1681 ttatctagaa aagtga
//
LOCUS       DACDVT010000182         1671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDVT010000182 DACDVT010000000
VERSION     DACDVT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.190"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1188)
                     /locus_tag="IWL52_002755"
     CDS             complement(<1..1188)
                     /locus_tag="IWL52_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779251.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKES"
     gene            complement(1286..1561)
                     /locus_tag="IWL52_002756"
     CDS             complement(1286..1561)
                     /locus_tag="IWL52_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9779252.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT          527 a          319 c          197 g          628 t
ORIGIN      
        1 actttcctta ctatcatcaa aaaaattaac atcagaaagc aagacatcat agacacgtcg
       61 catttcctct agataattcc ttattgtcct gaaaagtttg tctttcattg attcaagagt
      121 gtcaaaaagt acttttgatt cctctttcaa ttttcgaaaa tgtgtaggat acaagtctag
      181 gttctctaat agccaattaa atgtttctac ccgactgtct ttctttaaaa ttagcatcac
      241 aactccaata aaattttcac tgatatcaca attactggaa tgacaataaa aaatggaatg
      301 cccagtttta ctggtaaaaa tactagcaga gggattatga tcgttgtgaa acagacatct
      361 taagttttta cttgacttaa gtaatttatt catatcaatg gttaacaatc tttcataaat
      421 ctcattaaaa gacatgtcgg aggaatcaaa caggcagtct ttaaaccact gtttcatttc
      481 ttctttgtcg tcatttttta tcaacttaac aaaatcggtt tcattgacta gttcatactc
      541 tctccctgaa tcactttgac aatttttaga acatgtcatg gtaaaatcaa tcggaagctc
      601 agtaatatct aacatgttgt cttcattaat tattatcact gcatctgact tatatcctcc
      661 aaaaaacatt ctattcgaag catttacatt gtcatcacac ccggggatgt tctttttcaa
      721 gtattcgtaa acagcaacca ttttaccata gtcagtaatt gcttctttta atagaaagac
      781 aatcttgtat ttttctagat tttcagagta ccgaatactt ttttgtatcg caaacgcaga
      841 attatgaact aaattgatag ataaaacctt ttctacgctt aagtatctac tatcatcttt
      901 actcaaatct actaccttcc ccttctcatc tttaacagtg ttatcgacat ccagaataat
      961 tactctggtt tccacaaaac tctccttgtc attgccttta ttctttgtct tgcttggaat
     1021 aaatgattgt gatgaattta gtatctctac aaactcatct cttgtaactt ctctatagtt
     1081 atctaaacac ctatctctta catcagtttt ttcttttttt gtaactggct tctctaataa
     1141 tttttcttga tctacataaa ttggaatatt aatcattgat gtttccattt gtgtttcctc
     1201 cttataaaat tctgttaaag actaaaacat acagataata aaaacaaact ctttattact
     1261 atatgtttta gtcttatttg tttactcaac ctttataccg ttccatgaaa ctatcaatat
     1321 cagatttttt aaatctaatg accgtatcaa tttttataac aggtaacccg tattgagaaa
     1381 catattttct aatcgttcct actgatacac ctagatattc acttgcctgc tgatagtcca
     1441 tatactctag agtgactcca gaaataatgt cttctagcat aggctttagc actgtaatta
     1501 tctctttcac ttgttgttca acttgtatag aaacaatgct atcaatgatt tgtagagaca
     1561 tatttccccc tcctttcgtg attgcttgaa caaattttct aagaattatc ttcgtatttt
     1621 ttgctcaaaa taaaagtatt ttgttattaa ccgattaatc aaacaaaaaa t
//
LOCUS       DACDVT010000183         1572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDVT010000183 DACDVT010000000
VERSION     DACDVT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.191"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            210..476
                     /locus_tag="IWL52_002757"
     CDS             210..476
                     /locus_tag="IWL52_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9779253.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="IWL52_002758"
     CDS             503..802
                     /locus_tag="IWL52_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9779254.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="IWL52_002759"
     CDS             976..1527
                     /locus_tag="IWL52_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9779255.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
BASE COUNT          576 a          218 c          316 g          462 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tt
//
LOCUS       DACDVT010000184         1571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDVT010000184 DACDVT010000000
VERSION     DACDVT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.192"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..527
                     /locus_tag="IWL52_002760"
     CDS             <1..527
                     /locus_tag="IWL52_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779256.1"
                     /translation="YGDEKRTKLSPYTREKLDELTQYVLTKEPEYQEYIQISKALKEE
                     AKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIEGVNQMQSIYNE
                     RSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAY
                     EEQQRRIQMEQERQ"
     gene            539..874
                     /locus_tag="IWL52_002761"
     CDS             539..874
                     /locus_tag="IWL52_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779257.1"
                     /translation="MKQYLNQWKVIEGSLKKERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLVPYYTTWEMNEEGINNFPCKSI
                     KESMSFTEC"
     gene            875..1270
                     /locus_tag="IWL52_002762"
     CDS             875..1270
                     /locus_tag="IWL52_002762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779258.1"
                     /translation="MDKKKRLEIDGGKVMIENKLTEKQEKFIDKFYFVTEKYLFPKTK
                     GKYFLKGSIGAILFFIVEFFSVFKSFGGYQKQIQESKELQHYFQMLTHSPLYFVMWIA
                     LFLLAFIIGYIGLTLAHILIDWVRGNLLK"
BASE COUNT          619 a          236 c          305 g          411 t
ORIGIN      
        1 attacgggga tgaaaaacga accaaacttt caccatacac aagagagaaa ctcgatgagt
       61 taacccaata tgtgttaaca aaagaacctg aataccaaga atatatacag atttcaaaag
      121 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
      181 aaaaaaacgc cctctttgat ttaaaaaagc gcacaggaaa tgccatccta acagaactga
      241 agagacagga tagtcaaata aaaccattaa tagaaggtgt caatcagatg caatctattt
      301 acaatgaacg atcctttcga acgcttaaac aattgccaac gaatgaatac aaatatagtt
      361 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg cagcggagtt
      421 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccatgca agtaaagcat
      481 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
      541 gaaacaatat ttgaatcaat ggaaagtcat agaaggatca ctaaaaaaag aacgaatcga
      601 acaacttccc gattgtttgg aaaaagagca tttgtttcag ataagagaaa tgttgagaaa
      661 cgaacagttt gatccaaatc aatttttagt tgttgagtac ccagctactg gcgtgtactg
      721 ttgtaatcat gtaaatggtg aaaaatattt tatcattcaa gaatatgagg gaaaattagt
      781 tccttattat accacctggg aaatgaatga agaggggatc aataacttcc catgtaaaag
      841 cattaaagaa tctatgtcat tcacggaatg ctaattggat aaaaagaaga ggcttgaaat
      901 agatggagga aaagtaatga ttgaaaacaa attaacagaa aaacaagaaa aatttataga
      961 caaattttat tttgtcactg aaaaatattt gtttcctaag acaaaaggga aatacttttt
     1021 aaaagggagc attggggcaa ttctcttttt tattgtggaa ttcttttcag tgtttaaaag
     1081 ctttggaggt taccaaaaac aaattcaaga atcaaaagag ctgcaacact atttccaaat
     1141 gttaactcac agtccgcttt attttgttat gtggatcgcg ctatttctac tcgctttcat
     1201 tataggatat atcggcttaa ccttagcaca tatcttaatc gactgggtca gagggaatct
     1261 actaaagtaa aagaaaattg ttctacttac ggaatgttaa ttgaataaaa gaagtaaaga
     1321 gtcctgaaca taatttgtct aggactcttt tttcagtgaa ttgttttccg ccagttcact
     1381 gagtggtgcg gtttccaatt ttattatatc aaatggaagt gagattccaa agggaaggga
     1441 ggaagaagaa atgactcatt caactattgg tgtcgttgtt gaaaaaagca gagacaatct
     1501 cgtttttgtg acagaaattc aaacagatag agcttttgta gtcacagaca aagctgcaaa
     1561 agcgtatcaa a
//
LOCUS       DACDVT010000185         1518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDVT010000185 DACDVT010000000
VERSION     DACDVT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.193"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..857
                     /locus_tag="IWL52_002763"
     CDS             90..857
                     /locus_tag="IWL52_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9779259.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            862..1518
                     /locus_tag="IWL52_002764"
                     /pseudo
     CDS             862..1518
                     /locus_tag="IWL52_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
BASE COUNT          554 a          258 c          252 g          454 t
ORIGIN      
        1 gttttacctt gaaaatattg tcttttatga tacaattaaa agagaattat ctttggaaaa
       61 aaattacttt atgaaagaag ggtcttcata tgaacaaaaa acgacgatta gaaaaaattt
      121 tagatatgtt aaagattgat gggaccataa ccataaaaga aataatagat gaactagata
      181 tttccgatat gacagcccgt agagaccttg atgctctaga agctgatgga cttttaacac
      241 gtactcatgg tggtgcacaa ttactttcct ctaaaaagcc acttgaaaag acacatatcg
      301 agaagaaaag tctaaataca aaagaaaaaa ttgacattgc taaaaaagcc tgctctttaa
      361 tcaaagatgg cgatactatt tttattggac ccggaactac acttgaacaa ctggcattag
      421 aattgaaagg tcgtaaaggt tataaaattc gtgtcattac aaatagtctc cctgtgttct
      481 tgattctaaa tgatagcgaa accattgatt tattgcttct tggcggtgaa tatagagaaa
      541 taactggagc ttttgtaggt tcaatggctt cgacaaattt aaaagcaatt agatttgcca
      601 aagcttttgt tagtgcaaat gctgttaccc ataattctat tgctacatat agtgacaagg
      661 aaggtgtgat tcaacaactt gccctaaaca atgctgtaga aaaattctta ttagtagaca
      721 gtactaaatt cgatcgatac gatttcttta acttctataa tctagatcaa ctcgatacca
      781 tcattacaga taaccagatt agccctcaac acttagagga atttagccag tacactacta
      841 ttttaaaagc ggactagaat tatgacttat aaaaatattg tttttgacct tgatgacact
      901 ctttatgacc atcaactccc ctttaaaagt agcgttgaga aatgttttcc aacgattgat
      961 atccaacaaa tagataacat ttacaaacgt ttcagatgtt ggtctgatgt tgcttttcct
     1021 aaatacacga aaaactcatc agtattgagc agcttagaat ttttcgctgt cagaagacta
     1081 tggaagaatt tggcatcgat agtatttcta ggacagaagc tctagacttc caagctgact
     1141 atgaatatga actcgatcag attacgatga ttcctgagat acatcaacta ctactggctc
     1201 tacataaaaa ccgcatccca ataggtattc taactaatgg ccctgttgac ttacagagtc
     1261 gaaaactcca aaatattggt gcctatcaat attttgaaaa gcaaaatatc atcattagtc
     1321 agtccactca cggaggagct gcagaaaata gctatgaagg taatgtaaaa aacttacaaa
     1381 gttaccaaaa tgtccaatta tctaaaaggg ctatagaaat tcttaaagag gaaattgagt
     1441 taaatcacca acatataaga ttcaatcctg attataaaga taatggatgg atttttactt
     1501 ccaaaagtcg tcataaac
//
LOCUS       DACDVT010000186         1484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDVT010000186 DACDVT010000000
VERSION     DACDVT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.194"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..413
                     /locus_tag="IWL52_002765"
     CDS             <1..413
                     /locus_tag="IWL52_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP9779260.1"
                     /translation="KKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVI
                     GEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWC
                     MIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            364..>1484
                     /locus_tag="IWL52_002766"
     CDS             364..>1484
                     /locus_tag="IWL52_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9779261.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSG"
BASE COUNT          507 a          214 c          314 g          449 t
ORIGIN      
        1 taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag
       61 tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc
      121 cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt
      181 gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg
      241 taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat
      301 cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca cattccttag
      361 cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta
      421 ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat
      481 aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat
      541 actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc
      601 ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata
      661 gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct
      721 aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac
      781 aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa
      841 cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg
      901 ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca
      961 tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa
     1021 aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat
     1081 caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt
     1141 ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat
     1201 aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg
     1261 ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt
     1321 aacgtaataa ataaactaaa tgagaaaaac attgaaacaa gagctttgtg gaaaccacta
     1381 catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg
     1441 tcagaaaagt tatttaatag cggcttatgc ttaccttctg gaag
//
LOCUS       DACDVT010000187         1450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDVT010000187 DACDVT010000000
VERSION     DACDVT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.195"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWL52_002767"
     CDS             <1..152
                     /locus_tag="IWL52_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9779262.1"
                     /translation="LASKACGADIQHIGDSLNGICLPENIFALGQAIRGYRHQIIMLG
                     KSNYR"
     gene            171..1421
                     /locus_tag="IWL52_002768"
     CDS             171..1421
                     /locus_tag="IWL52_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9779263.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQAITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT          456 a          225 c          255 g          514 t
ORIGIN      
        1 cattagcatc aaaagcatgt ggagctgata ttcaacacat aggggattct ttaaatggta
       61 tttgtcttcc agaaaatatt tttgctttgg gtcaagcaat tcggggatat cgtcatcaaa
      121 ttattatgtt aggaaaatct aattatcgtt aattaaggag gaaatgaaag atgatcgaca
      181 aagttcaaaa aatagctgca aaaattagta atcaaagaca tctactagct ttaagagaca
      241 gcatggctgc agttatgcca ttaatcatta ttggttcctt ttttatgtta ttagcaaatt
      301 tcccgataga aggatttaca aaatggttat ctgaaattgg agtacaatcc tttttgaaca
      361 aagcatcaga ttcaacattt ggtattattg gattagctgt tacctactct ttgggacaca
      421 atctagcaaa gacttatggt gtagactcat tatcagcagg attattatct ttgagtggat
      481 atgttttact tacaccttta ttggatgcag atggttctat aggatttcca acaaaatact
      541 ttggtacctc gggtcttttt gttggtatta ttgtagcact tgtatctaca gaaattttcc
      601 ggtggtttgt acttaaaaat attacaatta aaatgccggc aagcgttcca ccaaatgtaa
      661 gccgagcttt cgttgctatt attcctggct tttttgtcgt tctactatgg tttattgttg
      721 ttgtaatttg ttataaactt ggaattgaaa atgttcatgc tcttattgca gatacactcg
      781 caaaaccact atctttactg acgaaaactt taccaggaat tatattggtt atacttattc
      841 aatgtttctt ttggatgttt ggtattcatg gtgcacaggt aacggggcca ataattgaac
      901 cgttattatt gcaaaactct gatgtaaata gaattgctta tcaagctgga aaagaacttc
      961 ctaacattat aacttatgaa ttcttatata actttgtttt ttctggtggt gcaggttgtt
     1021 tatttgcact tgccatttta acatttttct tttcaaagag tcaggaaaat aaaactttag
     1081 gtaagttatc aatcgctcca gtaagttttc aagtagcaga gccccttcta tttgggtttc
     1141 caactatatt aaatataaaa atgttaattc catttgtaac tgcaccagta gtaacaacat
     1201 taattactta tttttcaatg agtatgggat tagttgcaaa acctgtagga gcaacaatcc
     1261 catggacaac tccaccgata attgccggat ttttagcttc tggtggacga ataagtggtg
     1321 cagtgattca agcaataaca attgcaataa atgttttgat atactatcca ttctttaaat
     1381 tagatgataa tgccaagcta aaaagcgaaa aaaatgatta gaactaccta tatgtttcta
     1441 actgtagtta
//
LOCUS       DACDVT010000188         1449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDVT010000188 DACDVT010000000
VERSION     DACDVT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.196"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            187..948
                     /locus_tag="IWL52_002769"
     CDS             187..948
                     /locus_tag="IWL52_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAP9779264.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1176..1370
                     /locus_tag="IWL52_002770"
     CDS             1176..1370
                     /locus_tag="IWL52_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779265.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          473 a          220 c          263 g          493 t
ORIGIN      
        1 tttgaccacc atatacatga cttttatttt tgtttctttt ttagaatcaa atgtgattaa
       61 atagaaaatt ttacaataat atgcacatat aaataataag atgatataat caacaaagaa
      121 aatgtgattt ttttcactat attttatcac atgaaaattc attaagaaag taggaatcga
      181 agtattatgc acacaccaga agaaattatg gaactcagta ttcatcttgg aacaaaaaaa
      241 atacataaac ctatcttagc taaactgatt ttaggtttta tcggaggagc catgatttct
      301 ctaggttatt tggcatacgt acgagtctct gcttctatcc cagaatctat ggctagcttg
      361 actagtttag ttggtgcctc ggtttttcct atcggcctgc tggtcatcct tcttggcggt
      421 ggtgaattga tcaccggaaa tatgatggct gtggcgattg cctttatgga taaaaaagtc
      481 acattctcac agctggttaa gaattggtca gtgattactt ttgcaaatat tattggtgca
      541 attttcgttg cctatttctt tggacacttt gttggtttaa ctcattcagg tgtctatctc
      601 gaccaagtga ttcatattgc tgatcataaa atcgctgttt cttggtggca agcattaggc
      661 tccggaatag gttgtaattg gtttgtcggt ttagcgttat ggttatcata tggagcaaaa
      721 gatgcggccg gcaaagtact aacgatttgg tttccagtca tgatttttgt ggcaatcggt
      781 ttccagcata gtgtggccaa ctgttttgtg atacctgctg ccatatttga aggtcaagcg
      841 acttggttag aatttctctc gaattttatc cccgtttata ttggaaatat catcggcgga
      901 gctatttttg tttcaggttt ttattacgtt agttataagc atcattaaga ttgaaggtaa
      961 tgttcattcg agcattacct tcaatctctt tatatactat ttatatttta agaacaatag
     1021 ccttttatta tatatattat tcaaattagt tatatcgagt gaaaaaaact ttcaaattga
     1081 ttctttttat ttatcataaa gatatagata cataatcatt ttcacttaca tatatttgaa
     1141 tattaactaa aaacagcaag aaagaagagg atcaaatgta ttatgtagaa gtaaaaacaa
     1201 aaggcgtcaa aaacaaacaa tatgtgaaag gtatcagtaa cgaatatcct ttacttggtt
     1261 catggaaaga agctgctcct ttttccaaac catgtgccat aaaaataaag aacgagctag
     1321 aaaaagaact gacttgtggc aaagctgttg tagatatcat cgaaaagtaa atccaatgta
     1381 tagaaaggaa gatcacacat gaaaagcgac gtacaaattg ctaagattat agaattagaa
     1441 gaagaagac
//
LOCUS       DACDVT010000189         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDVT010000189 DACDVT010000000
VERSION     DACDVT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.197"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(74..158)
                     /locus_tag="IWL52_002771"
     tRNA            complement(74..158)
                     /locus_tag="IWL52_002771"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(122..124),aa:Leu,seq:caa)
     gene            complement(176..247)
                     /locus_tag="IWL52_002772"
     tRNA            complement(176..247)
                     /locus_tag="IWL52_002772"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Gln,seq:ttg)
     gene            complement(257..329)
                     /locus_tag="IWL52_002773"
     tRNA            complement(257..329)
                     /locus_tag="IWL52_002773"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:His,seq:gtg)
     gene            complement(391..475)
                     /locus_tag="IWL52_002774"
     tRNA            complement(391..475)
                     /locus_tag="IWL52_002774"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Leu,seq:caa)
     gene            complement(496..566)
                     /locus_tag="IWL52_002775"
     tRNA            complement(496..566)
                     /locus_tag="IWL52_002775"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Cys,seq:gca)
     gene            complement(612..683)
                     /locus_tag="IWL52_002776"
     tRNA            complement(612..683)
                     /locus_tag="IWL52_002776"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Gln,seq:ttg)
     gene            complement(695..767)
                     /locus_tag="IWL52_002777"
     tRNA            complement(695..767)
                     /locus_tag="IWL52_002777"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:His,seq:gtg)
     gene            complement(770..843)
                     /locus_tag="IWL52_002778"
     tRNA            complement(770..843)
                     /locus_tag="IWL52_002778"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(809..811),aa:Trp,seq:cca)
     gene            complement(850..930)
                     /locus_tag="IWL52_002779"
     tRNA            complement(850..930)
                     /locus_tag="IWL52_002779"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(894..896),aa:Tyr,seq:gta)
     gene            complement(932..1007)
                     /locus_tag="IWL52_002780"
     tRNA            complement(932..1007)
                     /locus_tag="IWL52_002780"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(972..974),aa:Phe,seq:gaa)
     gene            complement(1043..1115)
                     /locus_tag="IWL52_002781"
     tRNA            complement(1043..1115)
                     /locus_tag="IWL52_002781"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1080..1082),aa:Asp,seq:gtc)
     gene            complement(1125..1197)
                     /locus_tag="IWL52_002782"
     tRNA            complement(1125..1197)
                     /locus_tag="IWL52_002782"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1162..1164),aa:Val,seq:tac)
     gene            complement(1213..1284)
                     /locus_tag="IWL52_002783"
     tRNA            complement(1213..1284)
                     /locus_tag="IWL52_002783"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1249..1251),aa:Glu,seq:ttc)
     gene            complement(1299..1387)
                     /locus_tag="IWL52_002784"
     tRNA            complement(1299..1387)
                     /locus_tag="IWL52_002784"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1351..1353),aa:Ser,seq:gga)
BASE COUNT          412 a          368 c          323 g          321 t
ORIGIN      
        1 atttggaggt aattacataa aaacaaaaaa aatatagcaa ctctctataa gaaagttgct
       61 atattgataa ttataccggc ggccggggtc gaaccggcac gtcctcaacg gacactggat
      121 tttgagtcca gcgcgtctgc caattccgcc acgccggcaa atttaaaaat aataactggg
      181 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
      241 taccccaaga ataaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc
      301 cggtgcgtta accacttcgc cacattcgcc atcataaaag atgttattta atttctcaat
      361 aaaaaactaa gatgaagaca tcttagtcaa taccggcggc cggggtcgaa ccggcacgcc
      421 ctcaacgggc actggatttt gagtccagcg cgtctgccaa ttccgccacg ccggcaagtt
      481 gatttattag tactaaggcg gtaaccggat ttgaaccggt gatgaaggtt ttgcagacct
      541 ctgccttacc acttggctat accgccatta tactattatc aatctacaaa ataaagaata
      601 aattatagaa actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg
      661 ccttaccgct tggctatacc ccaagaataa aaagggcgaa tgatgggaat cgaacccacg
      721 aatgccggag ccacaatccg gtgcgttaac cacttcgcca cattcgccat ggcaggggca
      781 gcaggaattg aacccacact aacggttttg gagaccgctg ttctaccttt aaactatgcc
      841 cctataaaat ggaggagagt ggattcgaac cactgaaccc taaggaacgg atttacagtc
      901 cgtcgcgttt agccacttcg ctactcctcc atggtggcgc gggacagaat cgaactgccg
      961 acacacggag cttcaatccg ttgctctacc aactgagcta ccgcgccata ttttaaaatt
     1021 attaattgaa taacaagaac gacggttccg acgggaatcg aacccgcgat ctcctgcgtg
     1081 acaggcaggc atgttaaccc ctacaccacg gaaccagttc attatggagg ttaacgggat
     1141 cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta aacctccaac
     1201 attcaattaa aatgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg
     1261 tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg gagagtaagg gattcgaacc
     1321 cttgagacag tttgcaccat ctacatgatt tccaatcatg ctccttcggc ctctcggaca
     1381 actctccaga gtgaaagaag ctcaatactc tttctcaaaa tgag
//
LOCUS       DACDVT010000190         1416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDVT010000190 DACDVT010000000
VERSION     DACDVT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.198"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(921..>1416)
                     /locus_tag="IWL52_002785"
     CDS             complement(921..>1416)
                     /locus_tag="IWL52_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9779266.1"
                     /translation="ELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTPAENLAYQV
                     GWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNI
                     DDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWK
                     RLAL"
BASE COUNT          444 a          269 c          205 g          498 t
ORIGIN      
        1 tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac
       61 acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga
      121 tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc
      181 ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcggt actgggatta
      241 aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt
      301 tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta
      361 agatctcttc agtatcagta ataggatagg cgataggcca catacccttt gatggttaga
      421 ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc
      481 aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag
      541 tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat
      601 ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct
      661 ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat
      721 cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc
      781 ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa
      841 agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt
      901 gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta
      961 aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc
     1021 gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc
     1081 attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct
     1141 ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc
     1201 catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat
     1261 tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc
     1321 acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat
     1381 ttagtataag tcctatttat ttcttttttt aattct
//
LOCUS       DACDVT010000191         1414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDVT010000191 DACDVT010000000
VERSION     DACDVT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.199"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            89..1381
                     /locus_tag="IWL52_002786"
     CDS             89..1381
                     /locus_tag="IWL52_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9779267.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          459 a          288 c          256 g          411 t
ORIGIN      
        1 tcttttttat tttccaaaac atatagaaaa gacaccttga aatcctttag aattaatttg
       61 gcgaaccaac ccaaaaggag gaatcaagat gtcttacact caccttatca aagaaacatt
      121 agatattcta gacttatcag ttacttttaa cgaaaattgt ctcacaaaag aaaaatacaa
      181 agggcaaatc tgtcatattt atcgtggaaa cttgatttat acagcccaag aatgcatcca
      241 ctgtaaacac cagattgctt cagatattgt gcgttggggt acaacaaccg tccggttgtt
      301 aatgaacgat gtttctgaat accgtaccta cctcgaattg aaaaagcaac gcttcaaatg
      361 caaagcttgt cagcggacct ttgtcgcaga tacttctgtt gcgaaaaaac attgtttcat
      421 cagtgagaaa gtccgctggt ctgtcgtgac tcgtctgaaa aaaaatacct caatgacaga
      481 gatcgccgct cagaaaaatc tatcggtctc ttctgtttac cgtattatga agcgatttta
      541 tcgtccgttg aatccttttc ggacaccgtt acctaaggta ctttgttttg atgaattcaa
      601 atcagttcgg ggtgtttcag gtgctatgag tttcatcatg atggatggac aaacacagcg
      661 cttattggac attgtcgaga accgacaact gcctttttta aaacgttatt ttagtcattt
      721 ttcacgagaa attcgtgaag cggtcgaatg gatcgtgatt gatatgtacg ctccttatgt
      781 atcgctcgtt aaaaagctgt ttccaaaagc acagctgatt attgaccggt ttcacatcgt
      841 ccaacatatt ggtcgtacct ttcgcaatca tcgaatcaaa gaaacgaacc aactattaaa
      901 aagcaaagaa caaaaacact atcaattagg taagcaatta aaaagatact ggaaacttct
      961 tcaaaaagat gaacgcaagc ttgattatac tcgacgcctt tggcgacctg gcttcaaagc
     1021 tcatttaaca gagaccgata ttgtggatcg cttactgaaa gggagccctg ctttgcgtgt
     1081 aggctatcag ttatatcaag actttcttta cgctgtaaag gaacgagact acgtatcgtt
     1141 tgaagaacta cttaccaata atatcatgct tcccgaaggc tatcagacga ccttgagaac
     1201 gttccagaaa tttctgccac aaatcaaaaa tgctttacaa caaagttatt caaatggccc
     1261 acttgaatgt ctgaacaacc atatcaaagt gttaaaacga aatgcatatg gctttcgtag
     1321 cttttataat ttcaaattgc gcatcatgat tcgtcatggc aacgcattga ttttcaacta
     1381 aaaaagatca agaggaactg atccacttga tctc
//
LOCUS       DACDVT010000192         1406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDVT010000192 DACDVT010000000
VERSION     DACDVT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.200"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..531
                     /locus_tag="IWL52_002787"
     CDS             <1..531
                     /locus_tag="IWL52_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9779268.1"
                     /translation="LLIIGIIFTTVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNK
                     SLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTFEGTVIESMTTR
                     IAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIE
                     KSYAQWDMINSKVSKK"
     gene            644..1279
                     /locus_tag="IWL52_002788"
     CDS             644..1279
                     /locus_tag="IWL52_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779269.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
BASE COUNT          543 a          222 c          234 g          407 t
ORIGIN      
        1 ctccttatta tcgggattat ttttactacc gtaggtttct cgctttcctc atcaaacgag
       61 gcaaatgaca ataacaaaca ggaaagctca tcatctgata ctcaaactac tacatctaac
      121 agcagcaata aaagcctaac tcaatttgaa gaatttttag agacaaataa tcatgactgg
      181 ggtgctttct tagatagtta ttattctatt actccagcta ctgagcaaaa tacagctttt
      241 actaaatata ttagtggtaa aacatatact tttgaaggaa ctgttatcga atcaatgact
      301 actagaatag ccattatagc agacaaagag tacgatgata aaagttggaa tgatatttct
      361 gcaacaccta aagtttcata tgtaattttt gcgaaggact taaacaatgc tgataccttt
      421 acaaagggcg ataaagtaac ttttacagga gaaattagtt caagaggatc gaacatagaa
      481 aaatcctatg ctcaatggga tatgattaat agtaaagtaa gtaaaaaata aatgaaccac
      541 gccccactaa gagttagatt gtgcacaata ataaaaccta tacaaaagag tgccatcagg
      601 agtcacaatg cttgtaaagg aaatgacaaa ataggaggta actatggata atagcaatga
      661 aaaactaacc aaaaaagaat atcttgaatt aatagttgaa ggcggtattg gagcaattcc
      721 ttatattggt ggcgttgtgc agtcacttta ttttgggtct aaaaacgaga agcgtttcaa
      781 acgcatcgag aaattctaca aaaatttaaa cgaatcatta gaaaacattc gcgatcaaat
      841 tccagaagat gttttcaact tagagaacaa ggaccaactc attggaatat tcgaagggat
      901 aaatgacgag gtagaaaaat caaaggcaca aaataaaata gatatgtata aaaatcttta
      961 taagaattgt ttacttcgaa ttaacaacgc ttcatgggat aacgaggagt attatcttct
     1021 tgtactgaat caattaacat cgattgagat tcaattgtta gcatgcttaa tgagtcgtgg
     1081 aaacgaaaat ttcactggaa atatcagcat gtctggctat tcacaagagc taatagatgg
     1141 tagcttaaat agattatctg attttggtct gttagaaaaa acgattagct ccatagtatt
     1201 aggtgatgtc ggcaaacaaa atatgggcta caaaatatct catttaggaa ttcaatttat
     1261 taactcaact cttacttaag ttttctttct aatatgacat ctagacctat cagcaatgca
     1321 acaataaaaa accagacata ccaataatca acaaataatc taaactctgg aagttcagtt
     1381 attcttttaa aaatcacttt aatcat
//
LOCUS       DACDVT010000193         1377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDVT010000193 DACDVT010000000
VERSION     DACDVT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.201"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            203..1288
                     /gene="xerS"
                     /locus_tag="IWL52_002789"
     CDS             203..1288
                     /gene="xerS"
                     /locus_tag="IWL52_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9779270.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          541 a          231 c          199 g          406 t
ORIGIN      
        1 aaaattaaga ttgcctcctt ctgtttcctt tcctattcat gtataccatt tattttaaat
       61 tattaaatct atacctattt atctgctttt ttgaaataaa ttaataaatt tcagagtata
      121 ccaattgaca aaacactcta taattggtat agtccaatta tagagatact cctctcccct
      181 acttatcgaa aggatgaata acatgaaaaa tacagaaata tatcaaaaaa tagatacaat
      241 catacttcat atgcccgatt atataaaaga ttttgttcaa gatcgtgagg ataaagatca
      301 atcacctcga actactttag aatatctaaa aaattataag ctcttttatg aatggcttct
      361 gagtgagtct atcgttcccg atacttcaat cacctcgata agacagttaa ctgcctatga
      421 tctaaacttg tacaaatctc atttgaaacg tcgcacaaag gaaaatgtaa aaaaagagac
      481 aacaaaaact aagctcaaag ataataataa cttaggtcta tctacttcta ctatcaatag
      541 aaatatcaca gcattaaagg ttctatttaa atatctttcg aaaagttcca ataatccttt
      601 aggaaaacca tacttagaaa ataatcctat ggatcaagta gcaactatca cagataaaac
      661 tacgttagct gcccgtgcaa acgcaatcga gaaaaaatta tttctagacg aagatacaca
      721 aaattatcta gattatattg ccaatgaata taaaaaaact ttgagcaaac gagctttgat
      781 ctattatcaa cgagatgtag aaagagacct ggcgatcaat gcattgattt taggatctgg
      841 attaagactc tccgaggttg taaatattaa tttagaggac ctatctttag aaaagaataa
      901 tgtggtagta actagaaaag gaaataagag agacgcagta aatatagcag catttgcaat
      961 ggaatattta gctaattatc tagctattcg gaaagaacga tacaaagtaa cagaaaacga
     1021 aaaagcttta tttctagcaa tttatcaagg agaagccaag cgtatatccg gtattgcaat
     1081 cgaacggatg gtagcaaaat attcaaaagg attcagagtc caagtcagtc cacataaatt
     1141 acgtcatact ttggctacta gactctatca acagacaaat tcattggtat taactgccca
     1201 acaattggga catacaagta caaatacaac aactttatat actcatatag acaatgcagc
     1261 tacaattgat gctttgaacg ctttgtaaat gtagtaaaaa atagtataat aatgaagcct
     1321 gtctctaaaa ttagataaaa gcccaacttt aaaggcaagc ttcattatca tttttat
//
LOCUS       DACDVT010000194         1366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDVT010000194 DACDVT010000000
VERSION     DACDVT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.202"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            55..798
                     /locus_tag="IWL52_002790"
     CDS             55..798
                     /locus_tag="IWL52_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779271.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(835..1332)
                     /locus_tag="IWL52_002791"
     CDS             complement(835..1332)
                     /locus_tag="IWL52_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9779272.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          430 a          239 c          230 g          467 t
ORIGIN      
        1 tttttcctag aggaaaatgt ctaaacatgt tggtaaatca acaaaaaaac gcatatagta
       61 gtctcaaaac tcccaagaaa tgaggactat ttaacaaact acgatggtta cgtcgtgaca
      121 actgaacatt ttgacttagc caatcctgtt actaaagtcg atgatatccc cgattatgaa
      181 atgtacagtc aaacaatcga tagcttaaat aagcgttttg gcaatcgtgt tttaaaaggc
      241 atcaaaatcg gttacatcgc ttctgaaaag gatcggatca tcgactattt agccgataaa
      301 gattacgatt taaaattact cagtgtgcat cataatgggc aatttgatta tttagatgac
      361 gaagtcaaag atatggatcc tgctattgtg attcctcagt attttgcaca actttcagaa
      421 gcactagttg ttatagaagc agatgtcttt gctcattttg actatggttt tcgggtattt
      481 ggtctatccg ttgctgaatt taaacaatat gaagcgcagt ttttaccgat tctagatcaa
      541 gtaatcaaaa ataaattggc attcgaatta aatgctaaga gcgcttactt gtatgacaat
      601 ttagcgttat atgaatatgt catcgattta tatctttcac gtggtggtac cttgtttagt
      661 gttggatcag atgggcatta ccttgaacat ttccgtttcc atttcgatga tctttttgct
      721 ctgttaaaag caaatggtgt gacggaatta gcgatttatc aaaaaggaaa gcgaattatg
      781 gttccattac cagtctaaaa aaacgtcctc agcatcaaaa gtgaggacgt ttttttagat
      841 ttctttttct aaaatcggac aaatacgatc ttgttttcct aaaggaaaat gtttgatccc
      901 gattttttta aatccatgac gttcataaaa acgaatacct gcatgatttt tttcataaac
      961 agtcaaacgt aaataacgtt tttgtttcgt tttagcataa cgattcgctt catcgagtaa
     1021 ccgttgccct aaccctttgt tttgataatc tttgagcaga tagagccgtt gaatctctat
     1081 agcatctttt tcatcattga ctttcatata accaatgagt atttgatgat ccttaatacc
     1141 aataaattta ctggttgact gtgccaattc tactgccaat atttcagttg tcaattgtgt
     1201 ctgtaaatat ccttgcatat caagtggatc tgcgccatcg ataaacgatt cataaaaact
     1261 gacaacactt acaacttgta aagcatctag atcatcataa tctaaatacg ctagtgtgat
     1321 ttgattgttc atctaactcc tccttttgac atagtatact ataaat
//
LOCUS       DACDVT010000195         1360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDVT010000195 DACDVT010000000
VERSION     DACDVT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.203"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..811
                     /locus_tag="IWL52_002792"
     CDS             <1..811
                     /locus_tag="IWL52_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9779273.1"
                     /translation="NDFSPKEQAIADYILENPSKVSHSPISNLASELGIADSTFFQFT
                     KKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESSISTLNDTKKL
                     LQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHSTDYHIQLMEA
                     SLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAKLGDVVFISIS
                     EETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISEIKE"
BASE COUNT          440 a          223 c          226 g          471 t
ORIGIN      
        1 caacgacttc agcccaaaag aacaggctat cgccgattat attttagaaa atccaagcaa
       61 agtaagtcat agtcctatta gcaatttagc aagtgaactg ggcattgctg actctacttt
      121 ttttcaattt acaaaaaaac ttggttataa tggatttaaa gattttaaaa tggccatgtt
      181 gatccaagaa aatgatctat ctgctatttc tatccacgaa aatattcaga aaacagatac
      241 tgagctcact atggcgcaaa aggtgttcga gtctagtatt tcaactttaa atgacacaaa
      301 aaagttgtta caacaaaagg atctaaaaaa agcggctact attatcaatg aatctaaacg
      361 tgtctatttt ttcggtgttg gcggttcaga gattgtagca actgacgctt atcataaatt
      421 tttgcgttcc cctatcgcaa cttctcacag cacggattac catattcaat tgatggaagc
      481 ttctttattg acggaaaaag actgtgctgt tttgatttct cacacagggc aatcaaaaga
      541 aacaatccat attgctgaaa ccgtaagaaa aactggagca aaaacaatcg ttattactag
      601 ccaagctaat tcttcccttg ctaaacttgg cgatgtcgtt tttatttcta tttctgaaga
      661 aacagaattc cgatctgaag ctttagcttc ccgtatttct caactaagca ttttggactc
      721 tctatatgtg attttgatgt tttacaacga aaaagaatca cgtcgttcta tcgctaaagt
      781 tcgacatact atttctgaaa tcaaagaata gcaccataac agaaaaagcg aaaaatccat
      841 aaaacaaaaa aagccactac caaatatttg gtagtggctt ttttggttct gcaagggtgg
      901 tacagaacat caatagtcgg tgggttagaa cgtatgtcat cgcatctttg gagaaggttg
      961 tattggtatt attcgagtta cttgttagat gcgataacaa agttagtata cagtagtaaa
     1021 aaatttatct tttctttttt ttattatttt tgtttcttat tttctaaaaa attaaaaaaa
     1081 attttggtga attaagtatt tccttgataa cactggcttt tggcctcatt ttaggttatc
     1141 atttgtaatt ttatttcaca gttgatatga ttgttatgta aaataaaaaa gtttaggata
     1201 catcgaattt ttggggaaat tcttttgggg gtatcctaaa tcttttctat tatattccca
     1261 tattttctat tattggtcta gacgtatgat ggtaaacttt gtacctatct gattgctgaa
     1321 tcattaattg cttatcatta gccatctttg tttcaagttc
//
LOCUS       DACDVT010000196         1341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDVT010000196 DACDVT010000000
VERSION     DACDVT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.204"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..771
                     /locus_tag="IWL52_002793"
     CDS             <1..771
                     /locus_tag="IWL52_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779274.1"
                     /translation="MSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLEYLN
                     RLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEWVKI
                     CFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVCERL
                     RNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGIDET
                     IKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(847..1182)
                     /locus_tag="IWL52_002794"
     CDS             complement(847..1182)
                     /locus_tag="IWL52_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9779275.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
BASE COUNT          457 a          200 c          252 g          432 t
ORIGIN      
        1 atgagttctt actatggtaa aacttatgac atagataaat gtaataagtt actatcactt
       61 atgactttta taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc
      121 gaatacctaa ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc
      181 aatgtctatc gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat
      241 atccttccac ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt
      301 aagatatgtt ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa
      361 ctcagcaagg aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg
      421 tggcaaggga catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta
      481 cgtaatgttg gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt
      541 aaacaaggat tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa
      601 gcaagtagta gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa
      661 gaattgatat tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga
      721 gatgatggac ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat
      781 ccatagctct taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt
      841 tatttttcaa tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat
      901 atagtcaatt gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc
      961 ttcctcataa tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct
     1021 agaaagagct gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac
     1081 tgcctgttgt gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt
     1141 tcgcttttct ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca
     1201 gtatagtaca acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag
     1261 ttgaaataca tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta
     1321 agtaatctca gccgatacag a
//
LOCUS       DACDVT010000197         1329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDVT010000197 DACDVT010000000
VERSION     DACDVT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.205"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..401)
                     /locus_tag="IWL52_002795"
     CDS             complement(<1..401)
                     /locus_tag="IWL52_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9779276.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(588..>1329)
                     /locus_tag="IWL52_002796"
     CDS             complement(588..>1329)
                     /locus_tag="IWL52_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9779277.1"
                     /translation="VPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKELIER
                     DKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDVCK
                     VGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAG
                     FHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNKKGI
                     FTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          402 a          269 c          174 g          484 t
ORIGIN      
        1 taccataggt cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata
       61 ccacttgtgc tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa
      121 taaagtagga taatctttct tcttatctcc gataactgat caattgctgc tgataacttt
      181 tgatctgaaa ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga
      241 gtttgggaat acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta
      301 gcgtttcgtt ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca
      361 aattggtgtt gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc
      421 cccctttcca ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa
      481 acataaaaat atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac
      541 gattaggcta ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa
      601 ctgaaattgg gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt
      661 ctatctctag taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt
      721 gcaaaatctg caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact
      781 tcgtgatttg cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta
      841 tcatgaaatc cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga
      901 catctttcca tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc
      961 cctcccgaaa cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct
     1021 accttacaaa catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc
     1081 ggatcaagtt ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa
     1141 tccgaatctg gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt
     1201 tctatcaatt ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta
     1261 ttatgttttt ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact
     1321 gcaggtact
//
LOCUS       DACDVT010000198         1325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDVT010000198 DACDVT010000000
VERSION     DACDVT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.206"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            634..1325
                     /locus_tag="IWL52_002797"
                     /pseudo
     CDS             634..1325
                     /locus_tag="IWL52_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          470 a          251 c          233 g          371 t
ORIGIN      
        1 ttttgtatgg attttttcat ttacacaaaa tattttatac tctcaattag tagattaata
       61 cacttgtttt ttagaattgt aaataactca taaaaaatat tttgactttc aaaatcactt
      121 ttgctaccag aagttgaggc gaagtgtttt aagcatagtt gtaccctagt ttgaccgtta
      181 aaaataaata acgatcgcta gagtgcaact atgtttgttt gccctaccgt cagcgtcggc
      241 ttggcctctc acatccatta ccgtcggaat tatctccgtt acataccctt acaaaatacg
      301 ccctgtaagc attgcatgaa accaccgtgc atttgcaccg tatttccgcc ctagccgttc
      361 aaatcggtcg ctaccaccat tcagaaacac gtgcggtaat ggttacacgt tgctgtctcc
      421 tagcattagt tattgcagcg gctaatgctt aacctttcta ctttatcgat aatttaatat
      481 caggcagctt ggctcctacc tctgatacta ttcgtatgtc ttacaaacag aatcgtacca
      541 agctttatat tagttttatg gataagctac aattaactag acagaaaaat taaggtgtgt
      601 agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt
      661 tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa
      721 atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa gcaatctaaa
      781 acgagtggtt cattcaaaga aaaagataat cttacgcctg aacaaaaaga attgttagaa
      841 ctacgtaaaa gaaaccagca attagaaatg gaaaatgata ttttaaagca agcagcgctg
      901 atattcggac gaagagacaa gtaatcgatg cgaataagca tctttaccct atatcagcga
      961 tgtgcagaat attaggtcta tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag
     1021 acgaatcaga acttgaagaa gtagtcgctg aagaatttat ccgcagccga aaggcctacg
     1081 gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat tcagatcagc cgacgaaaaa
     1141 ttagtagaat catgaaaaat agaggattaa aatcgagcta tactgttgct tattttaaag
     1201 tacatcattc tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt aaattcttaa
     1261 gagacaaccc attagaagcg atcgtaacag acttgactta tgtacgagtc gggaaaaaat
     1321 ggaat
//
LOCUS       DACDVT010000199         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDVT010000199 DACDVT010000000
VERSION     DACDVT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.207"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            197..>1320
                     /locus_tag="IWL52_002798"
     CDS             197..>1320
                     /locus_tag="IWL52_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9779278.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGL"
BASE COUNT          506 a          210 c          280 g          324 t
ORIGIN      
        1 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
       61 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
      121 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
      181 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaacat tcatagctaa
      241 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
      301 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
      361 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
      421 tagccaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
      481 acttgagaga gaactgatta accaacaaac agcgcaacaa cgtttgtcaa acgaaattga
      541 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
      601 gcaacagttg gatagtgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
      661 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaatccgaga aattagcgaa
      721 agcccaagaa tatgtttctc aacaatctga aaatgcagag aaaacgatag atatcctgag
      781 acgacagtta gaagctacac agtctgagtt tggcgctaca tccacagaag caatgcagat
      841 ggaggcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
      901 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atctaatgga
      961 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
     1021 ggactctgct aatagtgtag gtagttccca gagtaaaata caagctaatt ttggtttgac
     1081 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
     1141 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggcgattt
     1201 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
     1261 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
//
LOCUS       DACDVT010000200         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDVT010000200 DACDVT010000000
VERSION     DACDVT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.208"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(15..1154)
                     /locus_tag="IWL52_002799"
     CDS             complement(15..1154)
                     /locus_tag="IWL52_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9779279.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          336 a          281 c          239 g          432 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttat
//
LOCUS       DACDVT010000201         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDVT010000201 DACDVT010000000
VERSION     DACDVT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.209"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            153..1201
                     /locus_tag="IWL52_002800"
                     /pseudo
     CDS             153..1201
                     /locus_tag="IWL52_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          507 a          143 c          245 g          369 t
ORIGIN      
        1 gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc tagtctacac
       61 accttaattt ttctgtctag ttaattgtag cctatccagt ttggaaaaat aaaaaattat
      121 atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct ataaaatgct
      181 attgtgtaaa aaataaagga ggaataattt atgaaaaaat agtaccaaga ggagcatgcc
      241 tattacttgc attatgtttg agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca
      301 ataaaataaa agaaggacaa tccataagtt ctgaagagga aggcatgtca attaatgcaa
      361 atggcaaaaa tgaaactttt aagccatcag attatacatt ggaggcaaag aaagaatatg
      421 tttatgaata tttagggctt aaatttaaac tctcagacaa atttagaaat tatatagctg
      481 ataaaaagat agcgatgtta gatgatcaaa gtcctataga taaagaacta aagtatgcta
      541 ttttgacatt tgagaaaatg acagaagaac aaaagaatgc agttatagaa aagatgggcg
      601 atggatataa aaattggcaa aatgagcttg aaagaattgg gactattggt atatttgaaa
      661 aaaatacatc agaagaaaaa aatctaaaat tataaaatgt gatactcata ctaaaatagg
      721 agtttcaagt gatggaaaat acgattgcta tttaagcaca aatagcggag ctgaaagtaa
      781 tcttcttgat gaattaaaaa gaactgagat tcaaattata gataaaaaag aacgtcctaa
      841 aaatggtttt gtgctatcag aaaaaactga tttagaaaat acagaagcat ttaacaaaga
      901 atcggttaaa gatttacgta aactaccaac aaaggacata aatggcaagg attttacaag
      961 taaagatttt gagaagtatg atcttactat ggtaaatgta tttgcaactt ggtgtacagc
     1021 ttgtgtaaag gaaattcctg atttggttga ggttcagaat gaaatgaaaa gcaagggtgt
     1081 aaatatcgtt ggagttgtta cagatcctgt tgatgataac ggtgaaaaca aggaagcaat
     1141 tgaaaagtca aagttaatac atgaaaagac aaaggcttca tatcctttct taatgccaga
     1201 tggaatggca acactttttt tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat
     1261 ggct
//
LOCUS       DACDVT010000202         1255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDVT010000202 DACDVT010000000
VERSION     DACDVT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.210"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(185..370)
                     /locus_tag="IWL52_002801"
     CDS             complement(185..370)
                     /locus_tag="IWL52_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779280.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT          439 a          193 c          175 g          448 t
ORIGIN      
        1 tgaaaagata ggacttactc ttagtaaagt atctgaacta ttaattttta aactatcatt
       61 tttatcctca actacatgca catctgttgc aacttcactt tctgtctcat ccagataaat
      121 gattaaggaa tcgggaattg caatatcttc agctgacgct ggaatagttg agctaaatta
      181 gtcatcatat ctttattata aaataaccaa cagcaaaaaa tacaatgaaa aacaaacttg
      241 gaactaatgt atcattaata aatgttgaaa aattcagcaa ctcattactg tatgatttta
      301 gcagcccaac caacatatac cctataaaag ctaataagat ggttaggaat cctttagaat
      361 ttttactcat tcatagacac ttccttaaat aacttttatt atagaaggta ttgtaaataa
      421 taatattgac aaaaagtgac tcaaaataaa aaaaaactta cattatactg gaatgaactg
      481 tattgatagt gtacctaaaa gctctactga cataagatcg tgtaatcggt aaagtaattt
      541 tattaattat acgggatgta atcaattaga gaataaacta atcataaaag gtacttttac
      601 attcataatt gtcgctttga gattctactt tggttcacta tctgacccaa ttgcacattt
      661 tcgatcatta caagtattgg tgtcgttaga acaactaaat aaaaactagc ggtagatttt
      721 tcagaatcta tgctaatttc tgtatggatg ttccagtgat ggcaaatttc tcttttcatt
      781 gtttttcttg tcgcatgata tgcctttttg tgttattcag ttaaactata gaaattcttt
      841 cctagacaga tcttgatccc aaattttacg tttttgagca tcgatatagt ttaaaatgac
      901 attactcata tctaaagagt ttagattttt taggactaaa actgtcagtt gttctttttc
      961 ttctttctgt taaagtattg tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa
     1021 caagttttgt gacacaaaaa aacgaatgac ttgaggaaat cacctcaatc attcgtttaa
     1081 aatgctatat attctttagt ttaagatacg gaaattaatg ccatcgacca cactagtaga
     1141 atgaacgaca tttacttggc ctttttttaa tttcgttatg tttagcatat aaaaatatta
     1201 tagagtttac ttacaatgct taaaacatat actgtataat ctgatcaatt ttagc
//
LOCUS       DACDVT010000203         1249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDVT010000203 DACDVT010000000
VERSION     DACDVT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.211"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..801
                     /locus_tag="IWL52_002802"
     CDS             <1..801
                     /locus_tag="IWL52_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9779281.1"
                     /translation="TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYNPEGQKV
                     TTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSKEEVEV
                     TISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKNGNFTVK
                     VPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQNKNRG
                     KSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            935..>1249
                     /locus_tag="IWL52_002803"
     CDS             935..>1249
                     /locus_tag="IWL52_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9779282.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYD"
BASE COUNT          503 a          162 c          253 g          331 t
ORIGIN      
        1 acaccaggta attacgaagg ggttatcgaa gtaacgtatc cagatggtac gaaagataca
       61 gtgaaagttc cagtagaagt gacagacaat cgttccgatg ccgataaata caatcctgaa
      121 ggacaaaaag tgactacaga cttaaataaa gagccagacg catctgaggg aattaagaac
      181 aaagaagatc taccaaaagg aactacttat acttggaaag agaaagtaga tgttagtaca
      241 gctggaaaca aaaaagggat tgtagtagta acatatccag atggatctaa agaagaagta
      301 gaggttacta tttctgtaga agataaaaaa gcaccgaata aacctcaagt tgatcctatt
      361 acagagggtg accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca
      421 ttaccaaacg ggagtcaata ccatgggaca gctgataaaa atggtaattt tacagttaaa
      481 gttcctaaat tagaggcagg aacaaaagtg atagtaactg caactgatga atctggtaat
      541 actagtgaac ctactaatgt agttgtttct tcaaacgaaa aagacagtga aaaagccgtt
      601 agtaaagata ataagactga caatcaaggt agcaagcaaa acaaaaatag aggcaaaagc
      661 agtcctcaaa agcagtcctc aaaagcttat cctaagacgg gagagattga tagtaatatc
      721 tttactatta gtggtggttt aatcctgtta ggaactttag ggttattagg gtataaaaat
      781 cgaaaaaaag agaatgaata gaaaatcaat ttcttaaatc ttctctgaaa agaggaagaa
      841 tctttttctt cctctttttg ttttttattt tgggagatag gttgttagtt aaaaatgaag
      901 atacttttca tagaagggaa aagaatgtga ttaagtgaaa aaaatagtaa ttattggatc
      961 aaaccattcg gggctagctg cagctcgatt tctaaactta tgtgattcaa attatgaagt
     1021 tattctgata gataaaaaat cagaaattag ttacttagaa tgtgggacat cgttgttcat
     1081 aaaaagagaa gttagcaatt tagaagatct tttttatcaa aatagaagag aactaaattc
     1141 taagataaaa aagttgtttt tggagacagc agttgacaaa attgatattc aaaataaaaa
     1201 gattatttgt gtaaaaaaag atggcacaag attttcccaa gaatatgac
//
LOCUS       DACDVT010000204         1225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDVT010000204 DACDVT010000000
VERSION     DACDVT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.212"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1225
                     /locus_tag="IWL52_002804"
     CDS             <1..>1225
                     /locus_tag="IWL52_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP9779283.1"
                     /translation="GTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAIQ
                     GLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKK
                     VQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQ
                     LQLKAIGKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQ
                     PYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNK
                     LVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLL
                     EVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACD
                     FKLWTTIAKKKLTATQ"
BASE COUNT          440 a          241 c          280 g          264 t
ORIGIN      
        1 aggtacgatc caccaagttg aaaccgagaa aaaaagtgcg gcaagtccac gtcttttttc
       61 tctatccagc cttcagtcta aaatgaatca gctcatgaaa gctagtgcca aagatacttt
      121 ggaagccata caagggttgt atgaagggaa atatctaagc tacccacgaa cagatacccc
      181 ttatattaca gaaggggagt atgcgtatct gttggatcac ttagacgaat acaaacactt
      241 tttaaaggcc gaagccattc ctactcctat tcatacaccg aatagtcgat atgtgaataa
      301 taagaaagtc caggaacatt acgcgatcat tcctacaaaa acggtcatga cggcagcggc
      361 ttttgaacaa ttaagtccac tgcagcaggc catttatgaa caagtgttaa aaacaacggt
      421 ggccatgttt gcggaaaaat atacctatga agaaacaacg atcttgacgc aagttcagca
      481 gcttcagtta aaggcaatcg gaaaggtgcc gctagatcta ggttggaaga agttatttgg
      541 aaaagagagc gacggaaaag agaaggaaga agagccgcta cttcctaaag tgacaaaagg
      601 agaaatggtg acggtggatc tacaagttct tgaaaaagaa accaagccac cacaacctta
      661 taccgaaggt acgttgatca ctgcaatgaa aacagctggg aaaaccgtgg atagtgaaga
      721 agcgcaatcc atattgaaag aagtggaagg aatcggtaca gaagcgacac gggcgaatat
      781 tattgaaacc ttgaaacaaa aggaatatat caaggtagag aaaaataagc tcgtggtgac
      841 gaataaaggg attttgcttt gccaagccgt ggaaaaagag ccgctgctta cgagcgccga
      901 aatgaccgcc aaatgggaaa gctacttgtt aaagatcggt gaacgaaaag gaacacaaac
      961 cacttttcta gccaatatcc aaaaatttgt ctctcactta ttggaagtcg taccagggca
     1021 aatccaatcc actgattttg gatcgacgtt acaagaagtg aaagcagcca gtgaaaagca
     1081 agaagccgcg cgtcatttag gaatttgtcc aaaatgccag gaacaagaag tcttgctcta
     1141 tcaaaaggtg gcagcttgta cgtctgaagc ctgtgatttt aaattatgga caacgattgc
     1201 caaaaagaaa ctcacagcga cacag
//
LOCUS       DACDVT010000205         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDVT010000205 DACDVT010000000
VERSION     DACDVT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.213"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..909
                     /locus_tag="IWL52_002805"
     CDS             <1..909
                     /locus_tag="IWL52_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP9779284.1"
                     /translation="RRKKLAQMIQENIKEELGLIATVGVGDNPLLAKLALDNEAKHNE
                     GFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRL
                     GVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQV
                     AIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEG
                     QEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSM
                     LEGARSITRSTLVGGHAGGNGGIKND"
     gene            902..>1214
                     /locus_tag="IWL52_002806"
     CDS             902..>1214
                     /locus_tag="IWL52_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779285.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HI"
BASE COUNT          437 a          191 c          281 g          305 t
ORIGIN      
        1 cggagaaaga agctggctca aatgatccag gagaatatta aagaagagct aggattgatt
       61 gctacagtag gtgtcggaga taatcccctt ttggctaaac tagccttaga caacgaagca
      121 aagcataatg aaggatttgt tgcggaatgg acgtacgaaa acgttcctga aaaagtatgg
      181 aatattcctg aaatgacgga cttttgggga attggctctc gaatgaaaaa aagactcaat
      241 cagatgggga ttatgagcat tagagatctt gctaactgga atccttatac gattaagaat
      301 agactaggtg tgattggctt acagctgtac ttccatgcta atgggatcga tcgaacagat
      361 atagcaatcc caccagaacc gacaaaggaa aaatcatatg gaaattcaca agtgttacca
      421 cgagattata ctagaagaaa tgaaatcgag ctggtggtaa aagaaatggc ggaacaagta
      481 gcgatacgca ttaggcagca taactgtaaa acaggttgtg tccatttaca cattggcacg
      541 tcgatcttag aaacgagacc agggttttca caccagatga agatccctat cacagataat
      601 acaaaggaat tgcagaatta ttgtcttttc ttgtttgata agtattatga aggacaagaa
      661 gtaaggcatg ttggaataac ctatagcaag cttgtgtaca ccgattcgct tcagctggat
      721 ttatttagtg atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag
      781 attcggcaaa aatatggttt tacgtcgatt gtacatgctt cttcaatgtt agaaggcgct
      841 agaagcatta ctagaagcac acttgttggt ggtcatgcgg gcggaaatgg gggaattaaa
      901 aatgattgat tacaatgatc gtggacgttt aaagtgggca gggttttatt tatctgatca
      961 tactgaagaa attgatgccg acaatgaaca acgaaccagt caaaatttag caaaagaaca
     1021 aatgacgact gaagagattg cggaagtatt gaacgctgca gtcataaaaa atagatcagt
     1081 atctattcaa aaagaagaac gaaacgctga aggatactat ccagaagata tcattggcaa
     1141 gatcttaggt agtgatgagc tggggatcta cgttggaaaa gacaaaattc actacgacga
     1201 aatacgtcat atcg
//
LOCUS       DACDVT010000206         1198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDVT010000206 DACDVT010000000
VERSION     DACDVT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.214"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1198)
                     /locus_tag="IWL52_002807"
     rRNA            complement(<1..>1198)
                     /locus_tag="IWL52_002807"
                     /product="16S ribosomal RNA"
BASE COUNT          250 a          362 c          284 g          302 t
ORIGIN      
        1 cgggtgttac aaactctcgt ggtgtgacgg gcggtgtgta caaggcccgg gaacgtattc
       61 accgcggcgt gctgatccgc gattactagc gattccggct tcatgcaggc gagttgcagc
      121 ctgcaatccg aactgagaga agctttaaga gattagctta gcctcgcgac ttcgcaactc
      181 gttgtacttc ccattgtagc acgtgtgtag cccaggtcat aaggggcatg atgatttgac
      241 gtcatcccca ccttcctccg gtttgtcacc ggcagtcttg ctagagtgcc caactgaatg
      301 atggcaacta acaataaggg ttgcgctcgt tgcgggactt aacccaacat ctcacgacac
      361 gagctgacga caaccatgca ccacctgtca ctttgccccc gaaggggaag ctctatctct
      421 agagtggtca aaggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca
      481 catgctccac cgcttgtgcg ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt
      541 actccccagg cggagtgctt aatgcgttag ctgcagcact gaagggcgga aaccctccaa
      601 cacttagcac tcatcgttta cggcgtggac taccagggta tctaatcctg tttgctcccc
      661 acgctttcga gcctcagcgt cagttacaga ccagagagcc gccttcgcca ctggtgttcc
      721 tccatatatc tacgcatttc accgctacac atggaattcc actctcctct tctgcactca
      781 agtctcccag tttccaatga ccctccccgg ttgagccggg ggctttcaca tcagacttaa
      841 gaaaccgcct gcgctcgctt tacgcccaat aaatccggac aacgcttgcc acctacgtat
      901 taccgcggct gctggcacgt agttagccgt ggctttctgg ttagataccg tcaagggatg
      961 aacagttact ctcatccttg ttcttctcta acaacagagt tttacgatcc gaaaaccttc
     1021 ttcactcacg cggcgttgct cggtcagact ttcgtccatt gccgaagatt ccctactgct
     1081 gcctcccgta ggagtttggg ccgtgtctca gtcccaatgt ggccgatcac cctctcaggt
     1141 cggctatgca tcgtggcctt ggtgagccgt tacctcacca actagctaat gcaccgcg
//
LOCUS       DACDVT010000207         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDVT010000207 DACDVT010000000
VERSION     DACDVT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.215"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWL52_002808"
     CDS             <1..1076
                     /locus_tag="IWL52_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9779286.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACDVT010000208         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDVT010000208 DACDVT010000000
VERSION     DACDVT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.217"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1176
                     /locus_tag="IWL52_002809"
     CDS             <1..>1176
                     /locus_tag="IWL52_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9779287.1"
                     /translation="VIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTV
                     EEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSN
                     EEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIK
                     SQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGI
                     SSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPL
                     VSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKE
                     ECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVA
                     "
BASE COUNT          395 a          177 c          206 g          398 t
ORIGIN      
        1 tgtcattcat gatttgctat tcaatgatta cagtgaactg actgaaaagg tttctgatta
       61 ctatgttcgg tcattgattt ctactttagt tatccaatgt aaccgtttac tgaaaggtca
      121 tcatttaact gttgaagaag aattcctctt caatagcatt cgctatatgg aaacgtacat
      181 tgtggcaaat ggtttaattg aagaattgaa ggcaaaattg aatcttccat tttatgacgc
      241 agataaagaa tatctatgtc gtcaactatt tgcacatcgt gtgacaaata atttaaaaac
      301 cagcaatgaa gagtacgcta tgagcgttca taaattgatt gaaagaatga gtgagattga
      361 aaaaattgat ttgactcaaa atgatcgatt atatcaatcc ttattgtatc atgtgccagc
      421 aatggtcctt cgtttaaaaa aaggtattaa aattcaaaat ccgttattag aaaatatcaa
      481 atctcaatat tcagaactct ttagcatagt atggtacgcc atgtccatcc ttgaagaaga
      541 atttgatgtg actttaacgg acgatgaagt ttccttaatt ttgattcatt ttcagattgc
      601 tattgaagat cagtccaaag caaataatat catcattatt tgccaatacg gtatttcttc
      661 tgcgcaatta atttacaata aagttcgacg gtttttacca gcaaaagata atgtagaaat
      721 atcaacttta gataagtttc atttagctga aattggggaa gttgatttga ttatttcctc
      781 tctttccttt gatgcaggtg gtataccctt agtaagggta tctccattgg taagtaaaac
      841 agattatatg aatattatgg aagcttatac taaatattta atagaagaac cttcgcgatt
      901 tattcaaaat gaactgattc ctaaagcggt taaagttccc accatatcta aatttgttca
      961 atcatcttta gtaaaaacag gagtacaggt ggaaagcaaa gaagaatgtt tggatttgat
     1021 gatttccgaa ctagaagcca attcattagt tactgaagag tttcgtgctt ctatcttctc
     1081 tagagagaaa atggggaata ctaatttaga aagtggtgtt gctttaccgc atgcgaatcc
     1141 atcaatggtc attgaatcca acatttttat tgtagc
//
LOCUS       DACDVT010000209         1170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDVT010000209 DACDVT010000000
VERSION     DACDVT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.218"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            123..>1170
                     /locus_tag="IWL52_002810"
     CDS             123..>1170
                     /locus_tag="IWL52_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9779288.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQF"
BASE COUNT          417 a          192 c          216 g          345 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc
//
LOCUS       DACDVT010000210         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDVT010000210 DACDVT010000000
VERSION     DACDVT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.219"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..312)
                     /locus_tag="IWL52_002811"
                     /pseudo
     CDS             complement(1..312)
                     /locus_tag="IWL52_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            388..543
                     /locus_tag="IWL52_002812"
                     /pseudo
     CDS             388..543
                     /locus_tag="IWL52_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            562..>1137
                     /locus_tag="IWL52_002813"
     CDS             562..>1137
                     /locus_tag="IWL52_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9779289.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIG"
BASE COUNT          418 a          130 c          204 g          385 t
ORIGIN      
        1 atttatgctt gttggcatca attacttgtc ttttcgtccg aatatcagcg ccgcttgctt
       61 taaaatatca ttctccattt cgagttgctt atttttcttt cttagtgcga tcaattctgc
      121 ttgttctggt gttaagttgt ctctttcttt gaatgagccc gttgattgtg cttgcttcat
      181 ccatttatcg aaagaagaag gcgtaagctc atactctcga ataatttctg cgcgaggctt
      241 accagcgaga tagagatcga cgatttgttg tttgaattct tttgaatagg ttcttcgttg
      301 acgtcttggc atgaaaaatc ctccagtgtg ttttttatta ttctacacac cttatttttt
      361 ctgtctagtc tagtgtagcc gattcaggat aaggataagt gtattaattg tggaaaatgt
      421 aaaaaagttt gccaaatgaa taaagattca gttgagaatt gcaatcatct ggagtgtatc
      481 agatgtggac gatgtaaaaa tgcttgtcca gtggatgcaa ttagttatgg agtaagaaaa
      541 tagagaggag tagttaggaa agtgataaaa aatgctatag catatattac aagaaagaaa
      601 aatagaactt taataatttt tatcatatta actatagttc tttcttgctt gtattcttgt
      661 ttaacaataa tgaaatcaag caatgaaata gaaaaggctt tatatgaaag ttctaattct
      721 tcaatatcaa taacaaaaaa agatggtaaa tattttaatg ttaatcaatt taaagatatt
      781 gaaaaattaa aagaaattga agaaaaaata attcaatatg atggattagc aaaattaaaa
      841 gatgctaaag tagttagcgg agaacaaaga ataaatagag aagatttatc tgacgaattt
      901 aagaatgttg tttcactcga agctacaaat aatactaaaa gaaatatttt atttagtagt
      961 ggagtattta caattaaaga aggtaaaaat atagaagaaa atgataagaa ttcaattatt
     1021 gttcatgaag aatttgctaa acaaaacaat ctaaaattag gtgatgaagt tgatcttgaa
     1081 ttactagata ttgaaaaaag tggaaaaata aaaagtcata aatttaaaat tataggg
//
LOCUS       DACDVT010000211         1107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDVT010000211 DACDVT010000000
VERSION     DACDVT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.220"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..558
                     /locus_tag="IWL52_002814"
     CDS             <1..558
                     /locus_tag="IWL52_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9779290.1"
                     /translation="NAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSH
                     PEMGHMLIKRHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEA
                     FYLAQCAYNTTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIIT
                     PELGDNAGIIGGLALARKAVRNKQP"
     gene            749..901
                     /locus_tag="IWL52_002815"
     CDS             749..901
                     /locus_tag="IWL52_002815"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779291.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT          350 a          161 c          231 g          365 t
ORIGIN      
        1 aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca
       61 tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa
      121 gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt
      181 tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt
      241 gaaaaacggt tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt
      301 gaagcctttt accttgccca atgtgcatac aatacaacac tgatgttttc gccagataga
      361 attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa
      421 tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgatagcta cattattacg
      481 cccgaattag gtgataatgc cggaataatc ggcggattag cattggccag aaaagcggta
      541 agaaataagc aaccataatt aatgaatgtt tcattgatac tcatgtcaaa agtatttaac
      601 cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc
      661 tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct
      721 tttttctttt tgttgtgcgc tgtatacctt gaggatattc actggtggcg tacacttaaa
      781 tagtaatata ttgtgttact ttatgtctgt ggtaatagtt atagttgctt accaatgtat
      841 gttcattttt cgaaatgttg acttcataat ccttggctta atgtttgaat ttttttggtg
      901 atagattttt tcagccgtat aaaataaatc gtaattctat tatagagagt tgtgattata
      961 aaaagatagc aagaaaaaaa aagaaagttt ctatacattt taaatatttt attcctattt
     1021 ctattgctaa aaaagtagaa attctatatt tacctttaac tctcgcttct cttgcaaatg
     1081 tagttagcat tttaatcgat aataaac
//
LOCUS       DACDVT010000212         1085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDVT010000212 DACDVT010000000
VERSION     DACDVT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.221"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            80..>1085
                     /locus_tag="IWL52_002816"
     CDS             80..>1085
                     /locus_tag="IWL52_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9779292.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     P"
BASE COUNT          365 a          208 c          212 g          300 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccct
//
LOCUS       DACDVT010000213         1081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDVT010000213 DACDVT010000000
VERSION     DACDVT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.222"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..744
                     /locus_tag="IWL52_002817"
     CDS             <1..744
                     /locus_tag="IWL52_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9779293.1"
                     /translation="PEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGL
                     FDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHK
                     SAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWK
                     QISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRN
                     NIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            826..>1081
                     /locus_tag="IWL52_002818"
     CDS             826..>1081
                     /locus_tag="IWL52_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9779294.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVA"
BASE COUNT          365 a          170 c          203 g          343 t
ORIGIN      
        1 cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac acccgatgtg
       61 attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta tcgtatgttc
      121 aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa accgaatgga
      181 catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg tgaaaaggat
      241 tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt aggtctgaaa
      301 cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat cacattgaaa
      361 ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa atcaatggtt tgaatctgta
      421 ccgaaaaacc tattcaaatc catcactttt gattgtggaa aggaattttc aaattggaaa
      481 cagatcagta atgtcaatga tattgccatt tatttcgctg atccaggaac gccgtctcaa
      541 agaggcctaa acgagaattc taacggattg ttacgtagag atggtttatt gaaatctatg
      601 gatttcaatt cagtagatga attttttatt caatctgtcg catctaaacg aaataatatt
      661 cctagaaaat cactggatta tcgaacacct ttggaagtat ttttgagtta cgtaagtatt
      721 gatgatctgt ctaacttaat ttgacaatta agaaaaataa aaaaagaata aaagaatttt
      781 taacgaatat aatatatact agtaagaaag aaaggaggaa atctattgtt tgaaaaactt
      841 agacagtcta aactaatgtt ttggtcgctt gaattattga tcatagctgc attgatcatg
      901 atttcatcag aaataaactt tattttcgaa ccgatcggga catttttttc cacgttattt
      961 gcacctgtac tgattgcagg ctttctttac tatttattga atcctattgt caagctgttg
     1021 atgaaagcaa aaatcaaaag aatatgggct gtcgcaattg tgctgctcct tttagttgca
     1081 g
//
LOCUS       DACDVT010000214         1079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDVT010000214 DACDVT010000000
VERSION     DACDVT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.223"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            80..>1079
                     /locus_tag="IWL52_002819"
     CDS             80..>1079
                     /locus_tag="IWL52_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9779295.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEA"
BASE COUNT          362 a          197 c          212 g          308 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac
       61 tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa
      121 agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt
      181 cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga
      241 tggaaacggg aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg tccgctttga
      301 acaatacaat cacatgcctt tggttatgcg cttaaaaaag cagcgctata cctgtaaaaa
      361 ctgtcgaacc cattggacgg ctcaaagtta ttttgttcaa cccagacatt caatcgcgaa
      421 tcatgtcaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaaattgt caggtatctt tgaccaccgt tattcgtacg ttgaaagagt ttaaaagcta
      541 cttaccaaag caatctaaga ggattctccc cagagtattg atggttgatg aatttcgttc
      601 gcacgcttcc atagaagata aaatgagctt tatttgcgca gatggcgaaa caggacaatt
      661 aattgatgtc ttgcctacgc gtaaattgcc tcgattaaca agctatttct taggctgcgc
      721 taatcctgaa gaagtaaaat ttttagtcac agacatgaac gccgcctact tccagcttac
      781 caaacgtatt ctgccaaatg cgaaagtagt gattgatcgg tttcatatta tcaaacacat
      841 gaatcaagcc tttaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagtaggaca
      901 gaaaagtcag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagttttcga gcaccaaaat acccatttct
     1021 tacggaagcg atgatgattg atcgattgct tggcttttct acgtccctga aggaggcgt
//
LOCUS       DACDVT010000215         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDVT010000215 DACDVT010000000
VERSION     DACDVT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.224"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>1073
                     /locus_tag="IWL52_002820"
     rRNA            2..>1073
                     /locus_tag="IWL52_002820"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACDVT010000216         1048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDVT010000216 DACDVT010000000
VERSION     DACDVT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.225"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            172..441
                     /locus_tag="IWL52_002821"
     CDS             172..441
                     /locus_tag="IWL52_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9779296.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            434..691
                     /locus_tag="IWL52_002822"
     CDS             434..691
                     /locus_tag="IWL52_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9779297.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          404 a          117 c          192 g          335 t
ORIGIN      
        1 aagaaaaagc aggatttgag gtaccagcac tcaaaaagtg acgtcacttt ttatcctaaa
       61 aactaaaagt gatagcactt ttaattataa gaagttagaa tattaatcat ttgcttaatt
      121 gtacaatata atgtacaatt gttttataga aataaataag gggtgaaagg aatggaagca
      181 gtagcttatt caaatttccg ccaaaattta cgtagttata tgaaacaagt taatgaggat
      241 gctgaaacac ttattgtaac aagtaaagat gtagaagata cagttgttgt attatcaaaa
      301 agagattatg attctatgca agaaacgttg agaacacttt ctaataatta cgtcatggaa
      361 aaaattcgtc gaggagatga acaattctcc aaaggtgcat ttaaaacaca tgacttaatc
      421 gaggttgaat ctgatgatta aggcttggtc tgatgatgct tgggatgatt atctttattg
      481 gcatgagcaa ggaaacaaaa gcaatataaa aaagattaac aagttaataa aagatatcga
      541 tcgttccccc tttgctggat taggaaaacc tgagccatta aagcatgatt tatctggaaa
      601 atggtccaga agaattacag atgaacatag actgatatat agagttgaaa atgaaacgat
      661 atttatttat tctgcaaaag atcactatta accaatcgga agtaaggaaa gggtcagaaa
      721 cttaaaagtt tttgatcctt attttattta ccctagtcat ttaaaaagct aatatagctt
      781 agtgttgatt gttattaatg aatgtgtttg ttacgcgtat tacggatata aggttagtaa
      841 aatcatttct aaagttgagg aaaagtaaat ataaatggct taaatttcaa caatttgaag
      901 ttgaatagat atgttataat actattgtag tgtgggatgt tagttactaa aggatgacgc
      961 ttatatatat gactgaatag aataagcaat aggtttaata atctatttta aattttttgt
     1021 actagtttta gttaattagc aaaaacaa
//
LOCUS       DACDVT010000217         1048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDVT010000217 DACDVT010000000
VERSION     DACDVT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.226"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..299)
                     /locus_tag="IWL52_002823"
     CDS             complement(<1..299)
                     /locus_tag="IWL52_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9779298.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIP"
     gene            complement(338..>1048)
                     /locus_tag="IWL52_002824"
     CDS             complement(338..>1048)
                     /locus_tag="IWL52_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9779299.1"
                     /translation="EMPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHA
                     NWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPT
                     VTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAV
                     HAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFERQDDVASVAYWYST
                     EASGNTKRHFTDRERLPR"
BASE COUNT          304 a          234 c          200 g          310 t
ORIGIN      
        1 ggaatattga aatattgctt tagcgttcct acaaaattga aattttgcca agcaagtttt
       61 ggtgttgatg tgatatgacc gaaactcgtt gatttgcatt gaatatcaat tggaccaaag
      121 cagccgaccc cgatgcctgc caattgttcc ttaaaaggtt taaaaaaatc aatgacctgc
      181 ttcattgttt caactggagt tgttgtggga aaactgactc tttccaaaat cgttaattcc
      241 tcagtaccga ctgcacagac aaatttagtc cctccagctt caattgcccc atatatcatc
      301 ataggttcct cccttcgaat atgtgaaaaa cacttcatta acgcggcaag cgttctcgat
      361 cagtgaaatg acgtttagta tttcctgaag cttctgttga ataccagtaa gcgacactag
      421 caacatcgtc ttgccgctca aataagcgaa catcatcgtt gccgatttgt tgtaaagtga
      481 ccgataaatc ttcggtaaaa cagatggggt cttgaagatg ccaacgataa agaccatgtc
      541 gtggcattgc gtcgtcacca aaggcatgaa cggcattcaa ttttcccgtt tcaaaacgat
      601 ctcttgtggc atctctattt gattggtaag gataccctaa aaaggctgtt gaatagtttt
      661 tcgcatgtgg acgtccgaac tcatcgtact catgaaaggc ccacgccccg ccaaaataat
      721 cttctgaacc tgttgaggtt acggtcggat actcactatc cccatcaatg tagaatttaa
      781 actcgccttc gccccaccaa taacgttcta ggcaggtaag cgcaaggtaa gtacccacgt
      841 aatagccttg accctttaat cgatcaatta acttataatc ctcctgtaat ttggtaatgc
      901 gttcccgatg ccaatttgcg tgaaaatata ggctattttc ttccaattcg tccaccaaag
      961 cgtaatttat cgtataaaaa aatgaggaca tatccttcgg atgtaaattc tcgatcgtaa
     1021 ttttagcgga tgtttggaaa ggcatttc
//
LOCUS       DACDVT010000218         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDVT010000218 DACDVT010000000
VERSION     DACDVT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.228"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            369..1001
                     /locus_tag="IWL52_002825"
                     /pseudo
     CDS             369..1001
                     /locus_tag="IWL52_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          343 a          182 c          182 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccactact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DACDVT010000219          982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDVT010000219 DACDVT010000000
VERSION     DACDVT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.229"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..567
                     /locus_tag="IWL52_002826"
     CDS             <1..567
                     /locus_tag="IWL52_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779300.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          339 a          167 c          212 g          264 t
ORIGIN      
        1 gaagaacaat tgtatttgga aagtgaaacg agtcaaacga aggtcacagt cagccataat
       61 cgtttagacg tgtataaacg tcgtcctgaa acggtaaaaa aaggaacgga gcttctacga
      121 ctagaaacag aaaaatacat catgaatgtt tacctagaaa ggagtgagct ggtcgaaaac
      181 tttgtcagct acacgatttg caggaaacac tattactcag aatcagaata ttattcaatt
      241 tttgagagca tggaattaat ggaagaagaa atggaagagc taaacagcaa agatgcgacg
      301 atagacgatt attaccttgt atttactaga tttttgacgt gtggaacgga atctatcgtg
      361 ttaatgggaa tggattatga actcgctgta cccgaagcat ttttgaggga atatgtagaa
      421 gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt agacacctat
      481 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
      541 cgatttgaaa ggaaaaaaag gcaataatca tttataccga aaagtgagaa tgtgtgaaat
      601 aaggacaaaa aacctagtaa ctaagggaat ggttcttatt tcgcaccaat cgctccaacc
      661 gcccccgccg cttaggtggt gcgaaataag cgggcaacac ccgataacct aagagaaaga
      721 aggagatcca tatgaataaa atcattcctc tattgttagt cctatttgtt ggcgaaatac
      781 ttatgcgcca tgctgcagct gtgcatttaa acttcgtagt atttttggca atactggtat
      841 tttgtttgat cttttggcgc tttgtattag cccttgtgag tctactattc aagatgattg
      901 cagccatatt tttcataggg ttatttcgat gaaaggagag agtactaatg ccaattgcaa
      961 taggaaataa acggttaccc gt
//
LOCUS       DACDVT010000220          972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDVT010000220 DACDVT010000000
VERSION     DACDVT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.230"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>972)
                     /locus_tag="IWL52_002827"
     CDS             complement(<1..>972)
                     /locus_tag="IWL52_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAP9779301.1"
                     /translation="VHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEI
                     ISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLLIVVT
                     LSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTR
                     NFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNA
                     PFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEV
                     FTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDIL"
BASE COUNT          297 a          208 c          168 g          299 t
ORIGIN      
        1 tctaagatgt cataaacatt aatattagga attgaaaagg aacgtggaag atccgaagtc
       61 gtgatgacca attttgtctt gtcaaaataa gtttcatcat tttggtcgat taattcacgt
      121 agttctctgt agtccttcgt gaaaacttcg atggattcat ccgataaatg atgacgaaaa
      181 acatctttca atttgtccga aatccccaaa cccgacatac aggaaatgat aatgttgcta
      241 ctttgagaaa agccttcaaa gtattgaaca ttgattgaat acttgtctgc tgcatcctcc
      301 gcaatctgtt taaatggcgc attcatcgtc atcttcaagc caatatcaag tgcaatggaa
      361 gtcgtcaaat tattcagaag aagtaactcc ccttttaaat cgttcttgat ctgtgtgtaa
      421 atttgattta aagagcccat atctacgatc agcactagac tatctgtatt cacttgccgt
      481 ttcacgaaat tccttgtcga ttcgacaatc tctgacacat tactctctac tggcatatcc
      541 aacgcttcaa aaacataatt gccgcataat tgattcacta ctgcttgaat gctgctagcc
      601 gtactatctc cgtgagcaag caacaggccc ttcagctcga tggattcatc tacataatca
      661 gataaagtca cgacaataag cagtcttaga aagttctcgt ccttttctga gagcggtggt
      721 aaatgctcca agaactgatt acataagtaa gtcgtccgag gtagatttgc agctaatgta
      781 cgatttatcc cggttaagtc tgtcttatag cttggggaaa tgacatagga tctgctaata
      841 atttctgcaa caggtccact cgtcgtcaat ccgtacgttt ttcctaaaac ggatacccag
      901 acctttctaa acatctgaat catcaatttt ctactcattg ataaaaaaga atccttatca
      961 atgtgatgca cc
//
LOCUS       DACDVT010000221          956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDVT010000221 DACDVT010000000
VERSION     DACDVT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.232"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(852..>956)
                     /locus_tag="IWL52_002828"
     CDS             complement(852..>956)
                     /locus_tag="IWL52_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9779302.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          323 a          203 c          156 g          274 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acccacaatt tcatttgcca taaaatcctc
       61 cttcaagatt aactacttaa caaaattata aagagctttc taataggggt caagccccta
      121 attaaagacc cctaaaaaca gacaaaaaga cccccaaaaa cagacaaaaa gacccctaaa
      181 aacagacaaa aagaccccca aaagattctg cacaccttgg gagagtaagc atacagccga
      241 cggtaaagag gtttaaaaag aggtttaaaa agagattata aaagaggagg caaaatttcc
      301 cgcctaaaaa actcctgctt ttttcctcaa acaagttcgc gaatttttct aaactgggaa
      361 actcgctccc tacggtcact aaaaatgtgt gatcgctacg ctctcacatt atactgcgca
      421 gtcgcctaaa ggctcctttg cttaaaaaca gttcataaag aactcctttg aaaagttctg
      481 agcgtttgtt taagccgttt taagacagtc ttagtataaa acccttatct tgttctcaaa
      541 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aatgttctat aagacctcta
      601 aaattagatc taaggcgttt taaagttgtt ttggtataat tatataccag aacgtttaaa
      661 acgccttaaa acgcaaatta tagccatatg tatttttacg gaagccggca cactcatttg
      721 caaagcttca gctttgcaaa ttcggattcg ctcactcgaa aattgatttg ttttgactct
      781 gatcgatagg tgtatttggt tctgttgcaa agttttaaat aaagaataaa atcccttacg
      841 gtatctatga tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac
      901 ccgaagagag tgccttcttt tcgggttttc ttatataatc ctcggatggc ttccat
//
LOCUS       DACDVT010000222          953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDVT010000222 DACDVT010000000
VERSION     DACDVT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.233"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            67..150
                     /locus_tag="IWL52_002829"
     CDS             67..150
                     /locus_tag="IWL52_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP9779303.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            275..>953
                     /gene="erm"
                     /locus_tag="IWL52_002830"
     CDS             275..>953
                     /gene="erm"
                     /locus_tag="IWL52_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP9779304.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTV"
BASE COUNT          366 a          159 c          148 g          280 t
ORIGIN      
        1 gtgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga
       61 ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt tttgaaacag
      121 actaaaaaca gtgattacgc agataaataa atacgttaga ttaattccta ccagtgacta
      181 atcttatgac tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt
      241 tatttacaga tgtaataact tcaggagtga ttacatgaac aaaaatataa aatattctca
      301 aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga
      361 aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa
      421 aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga
      481 aaaattaaaa ctgaacattc gtgtcacttt aattcaccaa gatattctac agtttcaatt
      541 ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat
      601 tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg
      661 attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt
      721 ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa
      781 cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct
      841 atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa
      901 tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg tta
//
LOCUS       DACDVT010000223          940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDVT010000223 DACDVT010000000
VERSION     DACDVT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.234"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(41..367)
                     /locus_tag="IWL52_002831"
     CDS             complement(41..367)
                     /locus_tag="IWL52_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779305.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(413..775)
                     /locus_tag="IWL52_002832"
     CDS             complement(413..775)
                     /locus_tag="IWL52_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9779306.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(812..>940)
                     /locus_tag="IWL52_002833"
     CDS             complement(812..>940)
                     /locus_tag="IWL52_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9779307.1"
                     /translation="QCVSSWQTGRTIPKPYQMKMLSEILSVPMNELFSDVFNKVNS"
BASE COUNT          272 a          210 c          114 g          344 t
ORIGIN      
        1 tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac attaacacct
       61 cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca atatttatgt
      121 tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct ccattttcat
      181 gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt aaaatctgtg
      241 ttaccaatgg ttttgatgac ttctctaatc cagatgtacc tgtcattttt gaaaaaataa
      301 tacctataat agccaagaca acaataccta taatgacaaa cttcttgttc atatttactt
      361 tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct tatcagtaca
      421 ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc atcaaaatcg
      481 tacccatttt ctcatctttt tctcgatata agtagcccat aataacagca acagcaccaa
      541 atacaacagg aataaatgct aacgatacta ccatagaaat ccaaccaata atcaaataca
      601 gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt gtctgctcag
      661 tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtagt gctccacaaa
      721 actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa tacatgtctt
      781 ctcctcctaa aatcattcaa ttgcttatta gttaactgtt tactttatta aatacatcag
      841 aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt tgatagggtt
      901 taggaatcgt tctccctgtt tgccacgaac ttacacattg
//
LOCUS       DACDVT010000224          934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDVT010000224 DACDVT010000000
VERSION     DACDVT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.235"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>934)
                     /locus_tag="IWL52_002834"
     CDS             complement(<1..>934)
                     /locus_tag="IWL52_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9779308.1"
                     /translation="TGNMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPV
                     LWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATG
                     VGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEK
                     DTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFA
                     TTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSRE
                     ALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQE"
BASE COUNT          277 a          206 c          158 g          293 t
ORIGIN      
        1 tcttcttgtg ttcgactata cacatccgca caactgattt taatgatatt cttcagctta
       61 ccaatattcc cactaaaggg atgagataac attaaagaca aggcttctct tgaaacaata
      121 atatcttttt gtaaaacatc cgcttcattt tgaaaaaata attgaatcag ctctaatcgt
      181 tctcgaatgg ggcgatcttc aaaagttggc aacataacac taatttgaat acgccgatca
      241 aaggtgtcta ataaataatt ttcacctttt tccgtcgtgg cgaaaataaa acgaacctta
      301 gcagaatgcc aatttacatt ttcaccgact ggacgatagt ttccagtatc aataaataga
      361 aagagctttt cttgattttc tttcgatagg cgatggacct catcgagaaa aagatagccg
      421 ttgttggccg ttgccaataa accatcggta tccttttcag cccccgtaaa ggcccccttc
      481 ttgtatccaa acagtgttgc cgaaagcagt tcgggattgt ccgcataatc ggcacagttt
      541 aagatagcaa atggtgcatc tttttcaata atttgctcat ggatagcgta ttcaaaaatt
      601 tttgtggcta aaaaactttt tccaacaccc gtggctccat ttattaatac actgagtccg
      661 ttaggcggat atttgacagc ggctatacat ttttgaatga cctctctcag acttccgtca
      721 tgaccaacta cagaatgaaa ggcttcgttt atttgctccg tattatctac aaccgcatat
      781 tcatctaaag gcttccacaa aacaggcttt cccggtaatt tctcgacctt tttatcttcc
      841 actaatctcg tcaaataatg gctggcattc tgacgagaga tacctagatt cttcgaaatt
      901 ttctcggcag tgacagaaga catattccct gtta
//
LOCUS       DACDVT010000225          922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDVT010000225 DACDVT010000000
VERSION     DACDVT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.236"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>922)
                     /locus_tag="IWL52_002835"
     CDS             complement(<1..>922)
                     /locus_tag="IWL52_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9779309.1"
                     /translation="QVIDLSGLWRFKLDTNDDLQEELALEPINEKESYPISVPSSYND
                     LFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGKFITQHKGGF
                     TPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKENLVNFDFFN
                     YAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILIAIYDEEAQLV
                     AEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGIRSVQVTNGKF
                     LINNKPFYFKGFGKHEDSYVNGRGLNEAVNI"
BASE COUNT          292 a          187 c          108 g          335 t
ORIGIN      
        1 aatatttaca gcctcattca aaccacgtcc attaacatac gaatcctcat gcttaccaaa
       61 tcctttaaaa taaaaaggtt tattattaat caaaaatttg ccattggtaa cctgaacact
      121 gcgaatacca aattcttctg tataggtatc gatcacttta ttatcatcaa ttaaactaat
      181 tttcatacta tataaataag cgtctagagg ttcccaaaaa attggattct taagctgaat
      241 cattcctttc tttccttcag cttcagctac aagttgagcc tcttcgtcat atatcgctat
      301 taagattttt ccggtccatt ctgtatcgat attgaattct acacttgcat ccaaatcagt
      361 tattcttgta ttaatagtaa tatcacttat atagtctttc ggagttgtat ataacttaac
      421 aggtctatga atacctgcat aattgaagaa atcaaaatta actaagtttt ccttttttat
      481 atccccattt tcctgtactg tttccttcat aatccccaca ggtaaagtac tatcgtcaat
      541 aatattgttt acagcaactg taattcgatt attcccgtct tttacaaacc gattaatttc
      601 agactcaaat ggtgtaaacc cacctttgtg ttgagtaata aactttccat tcaaaaaaac
      661 ttttgcttca tgtgttgcag atccaaaacg aagaactaat ctttcttcta ataatctttt
      721 aggaagagtc acttcttttt cataccacac ccatccaaca tgatttctaa tgttctctgt
      781 ttcaaataaa tcattataag aagagggaac tgaaatcgga taactctctt tttcatttat
      841 tggttctaaa gcaagttctt cctgtaagtc atcatttgta tctaatttaa atctccagag
      901 accgcttaag tcaattactt gc
//
LOCUS       DACDVT010000226          911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDVT010000226 DACDVT010000000
VERSION     DACDVT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.237"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..128
                     /locus_tag="IWL52_002836"
     CDS             <1..128
                     /locus_tag="IWL52_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9779310.1"
                     /translation="NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKN"
BASE COUNT          342 a          131 c          134 g          304 t
ORIGIN      
        1 tgaacaattt aatagaacaa gaccatcgac ctattaaacg acgaaataaa ttttatcaaa
       61 gtctccgtac agcctcttcc acgattaagg gcatggagac cattcgagga atatataaaa
      121 agaactgaag aaatggaacg ctcttcggct tttcggtgtc tactgaaatc aaggtattaa
      181 tgggaataac agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc
      241 gcaacagaac ccattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
      301 cgaaaagtga aaagagcaaa tataaaattt aataaaaata agaactaaat aggaaaacgt
      361 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
      421 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
      481 tctatttaac tcattagaca ttctatgctc atttttctcc aaatttgttt caactctttc
      541 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
      601 atgatatata tctacagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
      661 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
      721 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
      781 tattgattgc aaaattatgt gacaaaaaga actagtagaa gaaggaataa tggcttgata
      841 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
      901 tatattttag g
//
LOCUS       DACDVT010000227          905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDVT010000227 DACDVT010000000
VERSION     DACDVT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.238"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(129..683)
                     /locus_tag="IWL52_002837"
     CDS             complement(129..683)
                     /locus_tag="IWL52_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9779311.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          280 a          169 c           97 g          359 t
ORIGIN      
        1 gttctatctc tggtattcta ttgtaaatgt tactaaacgt tttaaatact tccgaaaatt
       61 cagttatctt cattaatctc accttctaag ttttcattat acacaaaaaa tatatcctaa
      121 tatctcaatt agcctcccaa tttaaaacca cgtaacgatg cccctatttc atcttcagtt
      181 ataccaatgt acctttttgt aattgcttgc gagctatgat taaaaataaa cattaaggta
      241 gcaatgtctc tagttttctt gtagtagtga tacccaaaag tttttcttaa tgtatgtgtt
      301 cctatatcat ctcttcctaa tttttctgct atatctgcat atatctgata gacacgatcc
      361 cttgttatat gtcgaccttg ttgccgactt ggaaataacc aatcattttc ttctttacct
      421 tcagtatata ataaaatctc ttctctcaaa ttatctataa aaatcggttt tcgcttacct
      481 gttttatgtt caacaataat aggcttactt ttacctcgta tgtctccaac ttttaatcct
      541 ataatatctg acatacgtaa cccattattg attccaaaca aaaataaaaa actatctctt
      601 tctggattac ttgttcttct tagccaaaac tgaaaatctt gtatctcttc atttgtcctt
      661 aacggttgta cattatagct cactattgtt tcccttctta aaaatattct gagttcatga
      721 ttatatatat attttaccat aatcatgaac ttatatatat actctgttaa aagtgaagaa
      781 aatcaagggg tttagtttat gattttatat catttcagta actcagaata ttccgacttt
      841 tttgtacaaa aaaattaacc ttacttatat gaagattcta agctcttatt taaattttag
      901 acggg
//
LOCUS       DACDVT010000228          901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDVT010000228 DACDVT010000000
VERSION     DACDVT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.239"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>901
                     /locus_tag="IWL52_002838"
     CDS             <1..>901
                     /locus_tag="IWL52_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779312.1"
                     /translation="YDYSENPNLMPYITEPWVGPLLGNGHTVKDSVKRVITHTKTRTA
                     NSGDILSLGLGIPCTAEANNRYLITTLRPSTTYTLSVTMSVGSDWTGETNTIGVRLRY
                     LNEQGGIELPINALIPANVERDKMVTHTFTGITKDNVTSITNCYVEIFSLNSEYKGTV
                     SVSYDVKLKAHYPNLLDGPYWLGKVPLGENIADPTVVFPHKTSEYMVYGRRNTENYIA
                     DQTYTISMKATKLTVQSFAVYIAAGRVKVGDMKPTEGLANTWELTFTVTKQHIDSGVT
                     NYLEIYQYPSATKGAVQIEWLKLE"
BASE COUNT          311 a          158 c          178 g          254 t
ORIGIN      
        1 ctatgattat tcggagaacc cgaatttaat gccatatatt acagaacctt gggttggacc
       61 tttgttaggt aatggacata cagttaaaga tagtgttaaa agggttataa ctcatactaa
      121 aactagaaca gctaattccg gcgacatatt atcgcttggc ttaggtattc cgtgtacagc
      181 ggaagctaat aataggtatc ttataaccac tttacgacca tctacgacat acacattaag
      241 cgttactatg agtgtcggtt cagattggac tggagagacc aatactatag gggttaggct
      301 tagatattta aatgaacaag gtggtataga actgcccatt aatgcattaa taccagcaaa
      361 tgtagaacgc gataaaatgg tcactcatac atttactggt ataacaaaag ataatgtaac
      421 tagcataaca aactgttatg ttgaaatatt ctcacttaat agtgaatata aaggaacggt
      481 tagtgtcagc tatgacgtta agttaaaagc acactaccct aacttactag atggccctta
      541 ctggttaggt aaagttcctt taggcgagaa tattgctgac cctacagttg tctttccaca
      601 caaaactagt gaatatatgg tttatggtag acgcaataca gaaaactata tcgctgacca
      661 aacatatacc atatcaatga aagcaactaa actaacagtt caatcgtttg ccgtgtatat
      721 tgctgctggc cgcgttaaag taggagacat gaagcctact gaggggttag ctaatacatg
      781 ggagttaact tttactgtaa ctaagcaaca tatagatagt ggggtaacta attatctgga
      841 aatataccaa tacccgtcag ctacaaaagg agctgttcaa attgagtggc tcaaactaga
      901 a
//
LOCUS       DACDVT010000229          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDVT010000229 DACDVT010000000
VERSION     DACDVT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.240"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWL52_002839"
     CDS             <1..888
                     /locus_tag="IWL52_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9779313.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDVT010000230          859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDVT010000230 DACDVT010000000
VERSION     DACDVT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.241"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>859)
                     /locus_tag="IWL52_002840"
     rRNA            complement(<1..>859)
                     /locus_tag="IWL52_002840"
                     /product="23S ribosomal RNA"
BASE COUNT          187 a          267 c          194 g          211 t
ORIGIN      
        1 tctctcaggg atcttacttt cttaaagaaa tgggaaatct catcttgagg tgggcttcac
       61 acttagatgc tttcagcgtt tatcccttcc ctacatagct acccagcaat gcccttggca
      121 gaacaactgg tacaccagcg gtaagtccat cccggtcctc tcgtactaag gacagctcct
      181 ctcaaatttc caacgcccgc gacggatagg gaccgaactg tctcacgacg ttctgaaccc
      241 agctcgcgtg ccgctttaat gggcgaacag cccaaccctt gggaccgact acagccccag
      301 gatgcgacga gccgacatcg aggtgccaaa cctccccgtc gatgtggact cttgggggag
      361 ataagcctgt tatccccagg gtagctttta tccgttgagc gatggccctt ccatgcggaa
      421 ccaccggatc actaagcccg actttcgtcc ctgctcgact tgttggtctc gcagtcaagc
      481 tcccttctgc ctttacactc ttcgaatgat ttccaaccat tctgagggaa cctttgggcg
      541 cctccgttac cttttaggag gcgaccgccc cagtcaaact gcccatctga cactgtctcc
      601 caccacgatt agtggtgcgg gttagagtgg ccataacgca ggggtagtat cccaccagcg
      661 cctccatcga aactagcgtt ccgatttcta cggctcctac ctatcctgta catgcggtac
      721 agacactcaa tatcaaacta cagtaaagct ccatggggtc tttccgtcct gtcgcgggta
      781 acctgcatct tcacaggtac taaaatttca ccgagtctct cgttgagaca gtgcccaaat
      841 cgttacgcct ttcgtgcgg
//
LOCUS       DACDVT010000231          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDVT010000231 DACDVT010000000
VERSION     DACDVT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.242"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..>854
                     /locus_tag="IWL52_002841"
     CDS             62..>854
                     /locus_tag="IWL52_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP9779314.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFT"
BASE COUNT          312 a          160 c          158 g          224 t
ORIGIN      
        1 taacagtcct tttacggtac aatgttttta acgacaaaaa catacccagg aggactttta
       61 catgacccaa gtacatttta cactgaaaag cgaagagatt caaagcatta ttgaatattc
      121 tgtaaaggat gacgtttcta aaaatatttt aacaacggta tttaatcaac taatggaaaa
      181 tcaacgaaca gaatatattc aagcaaaaga atatgaacga acagaaaacc gacaaagtca
      241 acgaaatggc tattatgagc gcagctttac gacacgtgta ggcacgctag aattaaaagt
      301 acccagaaca cgtgatggcc atttttcacc cacagtgttt gaacgttatc aacgaaacga
      361 aaaagccctc atggcttcaa tgttggaaat gtatgtatca ggcgtttcaa ctcgtaaagt
      421 atcaaaaatt gtggaagaac tttgtggtaa atccgtctct aagtccttcg tttctagctt
      481 aacagaacag ctagaaccta tggttaacga gtggcagaat cgtttattat cagaaaaaaa
      541 ttatccttac ttaatgaccg atgtactcta tataaaagta cgagaagaaa atcgagtact
      601 ctcaaaaagc tgtcatatag cgattggaat aaccaaagat ggcgaccgtg aaattatcgg
      661 cttcatgatt caaagtggcg aaagcgaaga gacctggaca acattttttg aatacctaaa
      721 agaacgcggt ttacaaggta cggaactcgt tatttctgat gcgcacaaag gattagtctc
      781 tgccattaga aaatccttca ccaacgtaag ttggcaaaga tgccaagttc acttcctaag
      841 aaatatcttt acca
//
LOCUS       DACDVT010000232          847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDVT010000232 DACDVT010000000
VERSION     DACDVT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.243"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..694
                     /locus_tag="IWL52_002842"
     CDS             23..694
                     /locus_tag="IWL52_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9779315.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          325 a          127 c          129 g          266 t
ORIGIN      
        1 taaaagttaa ggagcattgt gtatgacaat taaaactagt ctccaaaatc aagcttacca
       61 atctattcgc cgacaaatta tctatgctga cttagaacct ggaaaaaaag tatcagaaaa
      121 aaaattagaa gaaacacttt ctattggtcg tacaccaata cgtgaagcac tgattcaact
      181 ccgacaacaa ggtcttgttg atacaattcc tcaaagcgga aattatatct cattaattga
      241 tttagattcg gccaaaaatg ctcgatttgt cagagaacat ttggaacgac aaattatgtt
      301 agagtgttgt gccaaaatga ataaccaaaa aaaacggatt ttagaaacta taatttcaga
      361 acaagaaaaa gctgttagtc aaaaaaatgc tcgtagtttt tttcataatg ataatttatt
      421 ccataaagct tgttttgaaa ttgcagagcg agaagaagta tggcaatggt tagaaaataa
      481 caatacacac ttagaacgat ttcgatggct acgagtaaaa acggaaggat taaagtggga
      541 aaaaattatg gaacaacatt atcaactatt tgaggcatta acaaaacaag atccagaaga
      601 agctaacttt ctaacatcgt tgcatcttca tttaatgctt aatgagcaaa atattgtttt
      661 agctactcat ccaaaatatt tcaaaaatat ataactttta actcttttta aattaaatgg
      721 ttgttctgat cagaaaaata tcgcttgttt tcttattatg taatatattt cataaactat
      781 tctgcataac aactattgtt tgtacttttt acattggata actaattatg actgctgata
      841 acttttg
//
LOCUS       DACDVT010000233          844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDVT010000233 DACDVT010000000
VERSION     DACDVT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.244"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            109..810
                     /locus_tag="IWL52_002843"
     CDS             109..810
                     /locus_tag="IWL52_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9779316.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          303 a          149 c          145 g          247 t
ORIGIN      
        1 atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt
       61 aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat
      121 aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat
      181 gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc
      241 cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt
      301 ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac
      361 ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg
      421 actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat
      481 cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg
      541 cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt
      601 ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc
      661 accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc
      721 aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac tttctgtaag
      781 acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata
      841 aata
//
LOCUS       DACDVT010000234          825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDVT010000234 DACDVT010000000
VERSION     DACDVT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.245"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            56..>825
                     /locus_tag="IWL52_002844"
     CDS             56..>825
                     /locus_tag="IWL52_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAP9779317.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHFFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          262 a          177 c          165 g          221 t
ORIGIN      
        1 tcccttctgt agaatagagt taccacaaca cattcacaga aaagaggact tccatatgaa
       61 cgattttact acagaaattc taaagactct agcgaacaaa ggcgatttga atgaattatt
      121 ccgtgtccat ttggaaaaag ctgtcaatac gcttctcaaa acggagttaa cggctttcct
      181 cgattacgaa aagtacgatc gcattggttt taacacgggt aattctcgta acggctccta
      241 tgaccgtacg gtcaagaccg agtacgggga acttcatctc cagattccgc gcgaccgcaa
      301 cggcgagttc aagcaacaga ctgttcctgc ttatagacgg acgaatgata ccttagagga
      361 gaccgtcatt cacttcttcc gaaaaggtat taccatgtcg gaaatcgcag acttgattga
      421 gaaaatgtat gggcatcact acacgcccca aaccatgtcc aacatgacta aagtgctgac
      481 tgaagaagta aatgcattta aagttagaac tctaaatgat aagtatgtcg ctattttcat
      541 ggatgctact tatattccat taaaacggca aaccgtctcc aaagaagcaa tctatattgc
      601 cattggtata cgagaagacg gcactaaaga agtactaagt tatgcgattg ctccgactga
      661 atcgacctac gtttggaatg agttgttaca ggacatttac tccagagggg ttcatgatgt
      721 cttactgttt attaccgatg gcttaaaagg catgaaagat acgattcacc agatttatcc
      781 taaagctaaa taccaacatt gctgtgttca cgtttcccgt aatat
//
LOCUS       DACDVT010000235          809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDVT010000235 DACDVT010000000
VERSION     DACDVT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.246"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>809
                     /locus_tag="IWL52_002845"
     CDS             <1..>809
                     /locus_tag="IWL52_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP9779318.1"
                     /translation="DKIKNRRNTDQLKQRDTVIIACVIWGIINGYTSQRATYRAVCSV
                     LFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNR
                     QAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEE
                     ANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVF
                     SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          279 a          144 c          147 g          239 t
ORIGIN      
        1 cagataagat taaaaaccga agaaatactg atcaactgaa gcaacgcgat acagtgatta
       61 ttgcttgtgt tatatggggc ataattaatg gctatactag ccaaagagcc acgtatagag
      121 cggtttgttc cgtcttattt cctaatggtg actttcctag taggagtcga ttcactcgct
      181 taagttcaaa cttagcttac actatcaaga ttattcgata ctttttcatc aagaagctca
      241 ccaaaggtga actagtcgga attatagata gttttcctag tccattatgt aaaccagtta
      301 gaaatagaca agcaaaacta ttgaatcaaa tagccaaagt tggctataac tcaacaaaaa
      361 aatcttattt ttatggtctt aaaattcata tgatcgttac taagactggc tttccgatta
      421 cttactcaat cacgaatcca ggtgtccatg acgtgaaagt gttagagact ttatcggaag
      481 aagcgaatct tcccaatatc ctaggggaca aaggatatat tagccataaa atccatgaaa
      541 agcttgccct taagggcatt actatatccg ttccgccacg taaaaacatg gataaatcag
      601 aaaaactaga ccacagctta cttggaaaac aaagaaaaac agttgagact gttttttctt
      661 ccttagaaaa attaggttgt caaaatttca actcacgttc tgttaaaggg ctagagagta
      721 gatttgaaag catattactg gcttacagtg ttctattaag tcgagcacaa cgacgttttg
      781 aaggaacttt gagatattct ttaggatat
//
LOCUS       DACDVT010000236          788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDVT010000236 DACDVT010000000
VERSION     DACDVT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.247"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>788)
                     /locus_tag="IWL52_002846"
     CDS             complement(<1..>788)
                     /locus_tag="IWL52_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9779319.1"
                     /translation="NIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYIGVEIAEAIRK
                     RGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVT
                     TSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATT
                     FFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFNYNFYAAGLNL
                     KTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVG"
BASE COUNT          255 a          136 c           95 g          302 t
ORIGIN      
        1 cctactattc tctttgtatt tttttcaaag ctaattctta attttacacg actatttttt
       61 ctcaaaaaaa caggtctaat gaaatcttct atttctatat aatctataga ctcacttaat
      121 aatcgtgctg tttttaaatt caaacctgct gcgtaaaaat tgtaattaaa tatagaaaag
      181 ccatttgtaa attgggaacc agaaggtgta aaatcttgat tgtcaatatg ttgagttgca
      241 atataagctg ttcttagagc attagaaata gaaaaatcaa cactacttct attaatggac
      301 gtaaaaaaag ttgtagcaca atccccaata gcatatatat cttttatatt tgtttgctgg
      361 tattcattag ttagatatgc tccactaaca tctctcaaga gttccttttt gccaattgga
      421 ctattagata gaaatcctat agcttgtacg accatatcca tagagtattg ggtaccactt
      481 gtagtgacta ttttttcaat cttttttctt cctaaatatt ttttaggact ttctgataga
      541 caaagatgta ttccattttt tgatagtatt tcttcaactt tatctgaaaa actacggtcg
      601 taataagtcg acaacactct atctgcaaca tcgaaaagat atacttcttt ccctcttttt
      661 cttatggctt cagcaatttc aactccaata tatcctgctc caataattcc tactttttta
      721 atagtattat catctaaagc tttaaaaatc tctttagcat ttggaaaatt ctttatcgaa
      781 aaaatatt
//
LOCUS       DACDVT010000237          718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDVT010000237 DACDVT010000000
VERSION     DACDVT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.250"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          249 a          121 c          117 g          231 t
ORIGIN      
        1 aagtgaggat agttcacaaa atccattgtt aaacttcacg aaagtggtaa aactcaaaat
       61 gctttatata ggattacagc atcgctcttt cttcgattgc gcgttgggta aaaaatatta
      121 tgaagtaaaa attgacgatg acacgttcct tacggctcat cagatgaaac agctacaaaa
      181 acgaaatgat caactcgaag aaaatttaat tctaaaaaag cgattgccat attcacgcct
      241 cgctcgacga actattaaag gtcaagattt acctgaagat catccttttt aggaaattga
      301 agctttttat gttgcacaat gcatttataa tactacaatg atgttttcac cagatattct
      361 tctagggtgc ggtgtaatga aacagataca cttgcaaaag aaaattcacc gcgaatttac
      421 taaaattatg aataattatg ttaatcttcc gaaatttttt tccaaaatta agggatcaag
      481 ctggtgttat agattgttta aggttggcaa aaaaataact tctttttaaa caattataga
      541 aaaccgatac tctgttaagt tggttgacac ggtgtcgatt tttatgactt tcttattctg
      601 aaacttcact aacggacagt acctaaaaag tctatagtat ggacgtttac gagtactatt
      661 ttatcaaaaa tgctgctcat ttcaacttat atgttttagt gaactacccc cacttagc
//
LOCUS       DACDVT010000238          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDVT010000238 DACDVT010000000
VERSION     DACDVT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.251"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>712
                     /locus_tag="IWL52_002847"
     CDS             <1..>712
                     /locus_tag="IWL52_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9779320.1"
                     /translation="GCSLSLNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVI
                     AEHRFYYLKELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVS
                     ADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIE
                     NKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPF
                     DLSGGEKQRLLVSIASLSK"
BASE COUNT          280 a           84 c          126 g          222 t
ORIGIN      
        1 ggatgtagtt tatcgctaaa ctctaaatta attatattgg atgaaccgtc ttcaaatttg
       61 gacttcaaag cgactaagaa acttgctgac ttactttgtg atttaaaaaa taaaggattt
      121 actattgtta tagctgaaca tcgtttttac tatttgaaag aattaattga tcaagttcta
      181 tttgttaaaa atagaactgt aaaaactaca actattcagt tgttaaaaac tgaaaacaat
      241 aacgcattaa ggaaatttga catttttaat agttcgattg aagttcccta tatcgaaaag
      301 gtaagtgcag atgtattaaa agttgaagaa attacttatg aaaatattct aaaagacata
      361 aattttgatg tcaaagttgg agatgttatt ggtttggtag ggaaaaacgg tgtggggaaa
      421 acaaccttac taagaatact catgaagatt attcgcccta ccaaaggaaa gataatcgaa
      481 aataaaaaaa atttgtcatc gccgttttta gtaatgcaag aaatggatta ccaatttttc
      541 acagaatcag tgaggagcga gttagccctg gggaatgaaa tagttagtaa agatcagcaa
      601 gaaaaaattt taaaaaaaat ggaactttat aaaatgaaag atcaattacc gtttgattta
      661 tccggtggag aaaaacaaag attattggtt tctatagctt cattgtcaaa aa
//
LOCUS       DACDVT010000239          699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDVT010000239 DACDVT010000000
VERSION     DACDVT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.252"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..139)
                     /locus_tag="IWL52_002848"
     CDS             complement(<1..139)
                     /locus_tag="IWL52_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9779321.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EE"
     gene            complement(139..>699)
                     /locus_tag="IWL52_002849"
     CDS             complement(139..>699)
                     /locus_tag="IWL52_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9779322.1"
                     /translation="YQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIID
                     TFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGE
                     KILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLL
                     EKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          232 a          129 c           76 g          262 t
ORIGIN      
        1 tttcttcaaa ctcttttttt ccactgctac tataagctgt tatatcaaaa ttaatagaat
       61 tcttttttcc atagcttatt agatttttta gatgtctctt tccaattgac cctaacccaa
      121 aaaaaattac tttcatcatt aatttacctc aatgtcgtta attaagttaa taatatactt
      181 atctttttca aaagtatatt ggggttccat tttcttttcc aaaagcgtga aaaattcttt
      241 aatctcctct aaatatgcat cttcaataat agtatgtgaa taattattat catgcataac
      301 ctcatcgtac aattgaatat ctgtcatact agaagtttct tcattccatg aaaatagaga
      361 atccgggtta ccttcccatt ctattaaaat cttttcacca attatagtta aatttctttt
      421 agctactctt gaaacaacat ctacacttaa agttccaact ataccagttt catgctcaat
      481 aataagagtg aaactgtccg aataatcaat atcaagattg gataatttct gggtcattac
      541 agtaaacgat ttaactttcc caaatgtatc tataatccaa ggtaattcaa tagcgaatat
      601 ttctctacac ccattagttt ttttgtttga aacaaaaaaa ttgcgataat cttcccaagg
      661 atgccagtcg ggtaaatact gtcctacatg gtattgata
//
LOCUS       DACDVT010000240          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDVT010000240 DACDVT010000000
VERSION     DACDVT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.253"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(54..629)
                     /locus_tag="IWL52_002850"
     CDS             complement(54..629)
                     /locus_tag="IWL52_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9779323.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DACDVT010000241          686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDVT010000241 DACDVT010000000
VERSION     DACDVT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.254"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            28..>686
                     /locus_tag="IWL52_002851"
     CDS             28..>686
                     /locus_tag="IWL52_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779324.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     Y"
BASE COUNT          240 a          108 c          128 g          210 t
ORIGIN      
        1 taccgtatcc gatacttttt tcacttgatg tattggcagt cattcgacga aaatcattct
       61 gatatattat caagtataaa aaataaacgt caactattag aaaaatttgc gaaacatgcg
      121 ccatatacgc gtagtataga tagaaagaaa tggatcagcc tactaagaat ttcgttgcaa
      181 cgaatgaaac aaggccactc gattacgtat gtcccagaag agatgaacaa atttgtgcat
      241 ccattgataa caaaagaaga gtttacgcat tacatactag agcctttttt tgaagcaaac
      301 ttctggtcga tgaatcacat cactccaaca gagcaagctt acctttattt tatgtttggt
      361 gtaatgaaca cctatttgga agaagatttg ttggatactc cagataaagt acaagaaagt
      421 tggggctgga ttgaatcgtc tattgtagaa aattttgaaa aaaacttcaa gatttgtttt
      481 acaacttcag aaaaaaatta tctatatgtc aatttggcaa tgatccattt gtattcaagg
      541 gtttttggga caaaaaagaa ggtggatgct tttggaaaat cagcagaaga cagagatttt
      601 cagcatatat ttccagtgat gtatccaatc atgcaacagt tttttagtgg ggtagctcgc
      661 agaattccag agcttaatac ttatta
//
LOCUS       DACDVT010000242          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDVT010000242 DACDVT010000000
VERSION     DACDVT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.255"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..434
                     /locus_tag="IWL52_002852"
     CDS             66..434
                     /locus_tag="IWL52_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9779325.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          244 a          113 c          104 g          218 t
ORIGIN      
        1 tttttataca taactattgt ttttctggta aaataaatca aaccacttaa tggaggcgtt
       61 ttagaatgga aaaatttcag ccgatcatca atcagatcaa agaaaaagaa catcaacaaa
      121 agatcaaaga cctttttgct aagataacag aaacgtatcc ccagctagac acaactgtaa
      181 aatggaacca acctatgttt actgaccatg gtacctttat tattgcattt agttttgcta
      241 aaaatcattt ttctatcgca ccggaaaaag cagcgattcg tgcccttgaa aaaaatatcc
      301 aagaagcagg ctacgtatat acggataacg tcatcaaagt tccttggaaa agtgtaatca
      361 actgggaatt gattgaacaa ttgatttcat ttaatattga agataaaaaa ggtcatgata
      421 aattttggcg ataaagcttt atacgaataa accttttact ggcgaggaaa ctattcatca
      481 gcttatacag gttttgttca atcctatcct ttttatcatc cctggttaaa cacttaattg
      541 acaaagtatc tgctattttt ataaggagtc tttcttgtgc tgaaaaaaat cactgattga
      601 atggctttga attttccact tagtaaaatt attttttcag caattttcct gcaaaacttt
      661 tgaataagga aaaaatacg
//
LOCUS       DACDVT010000243          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDVT010000243 DACDVT010000000
VERSION     DACDVT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.257"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>665)
                     /locus_tag="IWL52_002853"
     rRNA            complement(<1..>665)
                     /locus_tag="IWL52_002853"
                     /product="23S ribosomal RNA"
BASE COUNT          132 a          203 c          137 g          193 t
ORIGIN      
        1 tatagttacg gccgccgttt actggggctt caattcgtac cttcgcttgc gctaagcact
       61 cctcttaacc ttccagcacc gggcaggcgt cagcccctat acttcatctt acgattttgc
      121 agagacctgt gtttttgata aacagtcgct tgggcctatt cactgcggct gatcgtatga
      181 tcagcacccc ttctcccgaa gttacggggt cattttgccg agttccttaa cgagagttct
      241 ctcgctcacc ttaggattct ctcctcgact acctgtgtcg gtttgcggta cgggccgctg
      301 ttttctcact agaagctttt ctcggcagtg tgacatcagg aacttcggta ctattatttc
      361 cctccccatc acagcttgtc cttaagaaaa gaagcatttg actcctcttc agacttactg
      421 cttggacaga catttccgat cgtctgcatt ccttagcctc ctgcgtccct ccattgctca
      481 aacaaaaaca acgggtacag gaatatcaac ctgttatcca tcgcctacgc ctgtcggcct
      541 cggcttaggt cccgactaac cctgggcgga cgagccttcc ccaggaaacc ttagtcattc
      601 ggtggacagg attctcacct gtctttcgct actcataccg gcattctcac ttctaagcgc
      661 tccag
//
LOCUS       DACDVT010000244          642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDVT010000244 DACDVT010000000
VERSION     DACDVT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.260"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..283
                     /locus_tag="IWL52_002854"
     CDS             <1..283
                     /locus_tag="IWL52_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9779326.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          219 a           89 c          119 g          215 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt at
//
LOCUS       DACDVT010000245          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDVT010000245 DACDVT010000000
VERSION     DACDVT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.261"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            38..277
                     /locus_tag="IWL52_002855"
     CDS             38..277
                     /locus_tag="IWL52_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779327.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          205 a          120 c          100 g          205 t
ORIGIN      
        1 gtgtatgcta aacgatacta taaggagtgt gtacataatg aaaagaattc tgtttatcag
       61 tttatcaaat ttattgttac ttactgcttg tgggacttct gcatcaaaga acataccttc
      121 agatacgcaa atttcgaata gtatcgattc aacaatttct acaaatgaga aaaattctcc
      181 ggtagaaact tccgatataa aagtttctac ctctgcaaaa aacagggtaa agaaactgtc
      241 agcacgtctc ctttatttca aacaaactct gaattagctt cttcatcttc ttcagcaaat
      301 aatgatgata gtttcgcgtt tactcctaac taaatgcaac ctttacaaat gctttacgat
      361 aacaagcctg agctaaatga tcctgatatc agttttgcat tttttgaaat gattggatcg
      421 gatgatcttt ttaaaactac tagtttgtct atccgaaaat aggacagttc tggcagtata
      481 ggtttttatc gagtttctcc accaggcctc gttacagaga cagattctct aggcgaacct
      541 tttgaataaa tcaaaaatca tgaacaatca ctgccaacta aataaagtgc cttcaaaagc
      601 ttggtcttct cgcttttgaa ggcactttat
//
LOCUS       DACDVT010000246          628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDVT010000246 DACDVT010000000
VERSION     DACDVT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.262"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..448
                     /locus_tag="IWL52_002856"
     CDS             119..448
                     /locus_tag="IWL52_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9779328.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            438..>628
                     /locus_tag="IWL52_002857"
     CDS             438..>628
                     /locus_tag="IWL52_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9779329.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTT"
BASE COUNT          242 a           71 c          119 g          196 t
ORIGIN      
        1 gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt aaatacatta
       61 tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag ggatttaaat
      121 gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta aagattttaa
      181 tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc tttatgaatt
      241 tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg atataattgc
      301 agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa acaaaatgca
      361 ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa tgactaaaga
      421 ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc attaagtatt
      481 taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt tatgatgaaa
      541 ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt atttggtcta
      601 gtgggtttaa atatgcgggc acaacata
//
LOCUS       DACDVT010000247          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDVT010000247 DACDVT010000000
VERSION     DACDVT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.263"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          222 a           78 c          111 g          198 t
ORIGIN      
        1 taattatgtg attgaatgcc gatttttgtt acgcgctatt tttaattatc tgtaagaaga
       61 ataattttat tttactcata ttcgttttca ctttgaagaa gtaaattgca atttcttata
      121 agttaagcag ctatgcaaaa ggctatttat tctattgtga aatataagat gtagttgaaa
      181 caggatatat agaatcaaaa tacgaatctt attttgattt ttctagtttt ttgaaaaaag
      241 caccctttga tcgttgtatt agtggaaaag agaaaaagga gcatacttat catgtcacaa
      301 aactacaact aaatatctaa acaacaccaa tttgatgcct attgaaaaaa agtattgcga
      361 aatgaagcca agtctattag aaaatgaaat aatagaataa aagaaaatgg gaaaccgctg
      421 aatgatgtaa atgaaggtaa gtatccacga actaattttg atgaacaaga tatctatttt
      481 ttatttgtat tggagatact agtttaacag aattaggcag aagtcgccca tcctctatag
      541 gtgggtgatg aatgccgttc ggtatgaggg ttaccgttgg taactcgaag accgacaact
      601 aaggcgtat
//
LOCUS       DACDVT010000248          561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDVT010000248 DACDVT010000000
VERSION     DACDVT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.265"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>561
                     /locus_tag="IWL52_002858"
     CDS             <1..>561
                     /locus_tag="IWL52_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9779330.1"
                     /translation="GRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQNPDKV
                     LELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGKVYSGL
                     IEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTD
                     ASHMLGMTGAMKRSELLPTSIAAGCDL"
BASE COUNT          174 a           84 c          119 g          184 t
ORIGIN      
        1 agggagagtt tctgggattg aagcatcggc tattggatgt aactggagtt ttgctcctat
       61 tgtagatatt aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaaa
      121 ccctgataaa gttttagaac ttagtttagc ttatatgaaa ggtattcagg agagtggaat
      181 tttgcctgca gctaaacatt tccctggtga cggtattgac gaacgagatc agcatctatc
      241 ttttagtatt aatagtttag gtacggaaga atgggataat tcttttggaa aagtatactc
      301 aggcttaata gaagctggat taccttcaat tatggcaggt catatccata tgccagctta
      361 ttcaaaacat tttaatcctg atctgaaaga tgaagattta cttcctgcaa ctttatcaaa
      421 agaattaatc actgacttac ttagaggaaa actcggattt aatggattag tagttactga
      481 tgctagtcat atgttaggaa tgactggggc aatgaaacga tcagaattat tgcccacttc
      541 tattgcagct ggatgtgatc t
//
LOCUS       DACDVT010000249          539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDVT010000249 DACDVT010000000
VERSION     DACDVT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.266"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..88)
                     /locus_tag="IWL52_002859"
     CDS             complement(<1..88)
                     /locus_tag="IWL52_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9779331.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSG"
     gene            249..>539
                     /locus_tag="IWL52_002860"
     CDS             249..>539
                     /locus_tag="IWL52_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9779332.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETG"
BASE COUNT          188 a           78 c           85 g          188 t
ORIGIN      
        1 tcccagaatt taaaataaaa tcagcgactt ttttttcaga cttcgttaaa aatggataat
       61 tttctttaat taaatagata taactcatac aaactcctct acaatttcaa cttttctcca
      121 ttatactcca aaataataaa aaaaactcct aaatactcca tttttatctt tacttttatg
      181 aaagcggatg ctatattgtt tttggagttg ttatccaaaa ataataaaaa attggagtgt
      241 ttaggataat ggataaaaag aggtttttag atcttatttc aaaaggtttg attgtttcct
      301 gtcaagcatt acctggtgaa ccattttaca aagaggaaga aagtttaatg ccttattttg
      361 ctttggctgc tcaaagggct ggagcagttg gtatacgtgc aaattctgtt cgagatatta
      421 ttgaaattag aaagtgtgta tcattaccaa tgattggaat tattaaaaga gattatccac
      481 cagaatctcc attcattact gccactatga gagaagtaga tgaattagta gaaaccggt
//
LOCUS       DACDVT010000250          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDVT010000250 DACDVT010000000
VERSION     DACDVT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.267"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWL52_002861"
     tRNA            complement(5..90)
                     /locus_tag="IWL52_002861"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWL52_002862"
     tRNA            complement(107..178)
                     /locus_tag="IWL52_002862"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWL52_002863"
     tRNA            complement(190..262)
                     /locus_tag="IWL52_002863"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWL52_002864"
     tRNA            complement(280..361)
                     /locus_tag="IWL52_002864"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWL52_002865"
     tRNA            complement(369..441)
                     /locus_tag="IWL52_002865"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWL52_002866"
     tRNA            complement(445..517)
                     /locus_tag="IWL52_002866"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDVT010000251          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDVT010000251 DACDVT010000000
VERSION     DACDVT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.268"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWL52_002867"
     CDS             complement(<1..132)
                     /locus_tag="IWL52_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9779333.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDVT010000252          512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDVT010000252 DACDVT010000000
VERSION     DACDVT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.269"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>512
                     /locus_tag="IWL52_002868"
     CDS             <1..>512
                     /locus_tag="IWL52_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9779334.1"
                     /translation="FSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNPQAF
                     VTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQYFGL
                     KFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILH
                     VEKGKTIHIQ"
BASE COUNT          149 a           95 c          116 g          152 t
ORIGIN      
        1 ccttttctgc tagttctttc aatccaacct atagcgagga aatcaaaggc atcacttggg
       61 gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagc gagctgcaca
      121 accctcaagc tttcgtaacc cgattagaag aactaatttg tttggcagat ttagacggtt
      181 cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg cgagactttg
      241 gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag cctaatgatc agtcaatatt
      301 tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt
      361 cttggaagaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt
      421 tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc
      481 acgtagaaaa gggaaaaacg attcacattc aa
//
LOCUS       DACDVT010000253          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDVT010000253 DACDVT010000000
VERSION     DACDVT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.270"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          148 a           79 c           81 g          198 t
ORIGIN      
        1 aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt aggtctagag
       61 ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt gtcgcggatt
      121 accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat ttggaatggt
      181 tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta ataccaaacg
      241 caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt gtatcaaaag
      301 ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc tttgtaatgt
      361 aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt agcatcgtac
      421 gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt cttaaatggt
      481 ttagctattt tcttttaatc cgataa
//
LOCUS       DACDVT010000254          503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDVT010000254 DACDVT010000000
VERSION     DACDVT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.272"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>503
                     /locus_tag="IWL52_002869"
     CDS             <1..>503
                     /locus_tag="IWL52_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9779335.1"
                     /translation="FSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAF
                     VTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGL
                     KFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILH
                     VEKGKTI"
BASE COUNT          148 a           86 c          116 g          153 t
ORIGIN      
        1 ccttttctgc tagttctttc aatccaactt atagcgagga aatcaaaggc atcacttggg
       61 gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagt gacatgcaca
      121 accatcaagc cttcgtaacc cgattagagg agctgatttg tttggcagat ttagacggtt
      181 catggtggtg gtggccgtat cgtttagagg ctaaacaagg agaagttgtg cgagactttg
      241 gctgcggcaa atgcggctgg gcatcgggcc tttttgtcag tataatgatc agtcaatatt
      301 tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt
      361 cttggaaaaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt
      421 tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc
      481 acgtagaaaa gggaaaaacg att
//
LOCUS       DACDVT010000255          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDVT010000255 DACDVT010000000
VERSION     DACDVT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.274"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>498
                     /locus_tag="IWL52_002870"
     CDS             <1..>498
                     /locus_tag="IWL52_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9779336.1"
                     /translation="SINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMK
                     VFQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDE
                     PFVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVE
                     SVFNEE"
BASE COUNT          192 a           53 c          100 g          153 t
ORIGIN      
        1 tccattaata atgaagatat caataaaaaa ctggtgaaga ttgcatatgt agagcaaaaa
       61 gctgatattg attttacttt tccaataaaa gtaaaagaat gtgtatctat gggaacatat
      121 gccggaatga aagtttttca aagaattaaa aatgcagaat ggcaaagagt ttcaaaggct
      181 ttggaaaagg tcgatatggg aaaatattct aatcatcaaa taggagaatt atctggagga
      241 caatttcaac gtgtactttt agcaagatgt cttgctcaaa atgcggattt catattttta
      301 gatgaaccat ttgtagggat agacttagtt agtgaacgaa taattatgga tacgttaaaa
      361 gaattgaaac atcagggaaa aacagtgcta attgtgcatc atgatttaag caaggtaaag
      421 aagtattttg ataatattat tcttttgaat cgaaatctaa ttgctcacgg aagtgtagaa
      481 tctgtattta atgaagag
//
LOCUS       DACDVT010000256          492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDVT010000256 DACDVT010000000
VERSION     DACDVT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.275"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>492
                     /locus_tag="IWL52_002871"
     CDS             <1..>492
                     /locus_tag="IWL52_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9779337.1"
                     /translation="ARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKE
                     LESFGLLLQKQMGFNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGM
                     KIIPTLDEKSDAESLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKD
                     RLQ"
BASE COUNT          211 a           77 c           90 g          114 t
ORIGIN      
        1 agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat gaagaaggta atgtttattt
       61 tacttatacg acaaatgaac tatgtagcgt attaaactgc caaaaacaaa aagcgatagc
      121 aatcaaaaaa gagttggaat cctttggttt attattacaa aagcagatgg gatttaacaa
      181 acagttaggg aaaaataatc ctaatagact atatctagca gaattaaaag tctcagaaaa
      241 tgatatctac ttactcgaaa aatttgatag agagaatagg gaaaatgttg ataaatcaga
      301 gggtatgaaa atcataccca ccctcgacga aaaatcagac gctgaatccc ttggggctca
      361 agagggtatg aaaatcatac cgtgccaaaa cgttgataaa tcagagggta tgaaaatcat
      421 accagaactt aataataata tattagacac taatagacac aatatagaca ctgaaaaaga
      481 ccgcctacaa ga
//
LOCUS       DACDVT010000257          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDVT010000257 DACDVT010000000
VERSION     DACDVT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.277"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>487)
                     /locus_tag="IWL52_002872"
     CDS             complement(<1..>487)
                     /locus_tag="IWL52_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP9779338.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACDVT010000258          481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDVT010000258 DACDVT010000000
VERSION     DACDVT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.278"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..476
                     /locus_tag="IWL52_002873"
     CDS             <1..476
                     /locus_tag="IWL52_002873"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9779339.1"
                     /translation="ELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGF
                     PNALVEAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYS
                     PQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
BASE COUNT          190 a           66 c           85 g          140 t
ORIGIN      
        1 aagaattatt agaaaattta ataaaagcat atcaactaca aaacgtaata tttttgctag
       61 gtggccaatc aaatccttat aaatacatgt cacgtgcaga tttatatgtc atgccatcta
      121 taactgaagg ttttcctaat gcattagttg aagcaatgtc tacagaaact gctgtcttat
      181 gtgcagattg caaatctggg ccaagagaaa tattatcgaa tagaacgcta gacacgattt
      241 gtgaaaatat agaatatgct gaatcaggca tattagtatg tccaatgaca gacagtagaa
      301 attattcgcc ccaatatgaa gaatgtgata aaaatttagc gaatgctata gaagaattga
      361 ttgaaaacga taatttgagg aataagtatg caaaagcagc aaaaaagaga gcagaactat
      421 atagttatga tgcctttgaa cataacttgt tgcaaattat gaagacattt aattaattca
      481 t
//
LOCUS       DACDVT010000259          478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDVT010000259 DACDVT010000000
VERSION     DACDVT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.280"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..383
                     /locus_tag="IWL52_002874"
     CDS             <1..383
                     /locus_tag="IWL52_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9779340.1"
                     /translation="TNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIE
                     SGISIVFEELDSYYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIA
                     AKVSSEKRLNSESSPSTITKDWLE"
BASE COUNT          192 a           71 c           74 g          141 t
ORIGIN      
        1 tgacaaacga cagcattgct acgtttgtcc ctgaacgagt attaaatttg ataaaaacat
       61 tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa
      121 aagctgaaat agaaagtggt atttcgatag tttttgaaga actcgatagt tattatgtca
      181 atgcagaaca agaattatac acgacactgt taaaagccta tcaaaaatta aaaaccgaaa
      241 aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa aaattggttt atcgaaaaac
      301 caatagctgc taaagtatca agtgaaaaac gtttgaattc tgaaagctcc ccaagcacta
      361 ttacgaaaga ctggttagag tgaaacttta ctgacaaagt gattgttgct tcatggagta
      421 acattaaagt taaaaatttt ttattattct aattatatta cgaatattac aaaataat
//
LOCUS       DACDVT010000260          470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDVT010000260 DACDVT010000000
VERSION     DACDVT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.282"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            42..470
                     /locus_tag="IWL52_002875"
                     /pseudo
     CDS             42..470
                     /locus_tag="IWL52_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          190 a           63 c           84 g          133 t
ORIGIN      
        1 cgggcaacag cagaaaagta tataaatttc cattagcttt aataaatcaa gagcaattga
       61 ttgaagataa agatatataa ccaggaagaa aatcaaacta gacatattcg taatttatct
      121 aatgctaaat tagaggaaga agcaagaaaa gttagtaatt ataataaaaa aatggctgca
      181 aaatatgtta acgaagagga gttttgcttc tcaaaaacaa aaatatataa acgtaatccc
      241 ttcgtatctg aatatgttaa acgacttgca aaaggtattt gtcaactttg ccaagaaaaa
      301 gggccattta taaaagacgg tgtaccttat ttacattgtc atcatataga atatttgtcg
      361 caaggtggaa aagatgtgat tgaaaactgt atcgcgttat gtcccaattg tcatgctaga
      421 attcatgaac ttgagttacc tgacgataaa gaaaaattgc tgaagatcgt
//
LOCUS       DACDVT010000261          469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDVT010000261 DACDVT010000000
VERSION     DACDVT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.283"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          161 a           65 c           64 g          179 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacc
//
LOCUS       DACDVT010000262          453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDVT010000262 DACDVT010000000
VERSION     DACDVT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.284"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          192 a           45 c           75 g          141 t
ORIGIN      
        1 aaaaactagc gaacaattaa atagaataac atccgttaat taaacgtaag tagcttttag
       61 atgaacgaaa aaacaatgaa atgctgtaat attcgtgttt aaaaataata aataaaaata
      121 gggaattttc tattgcaatt ttataaagag attgctatat tattatttgt tctaaatgat
      181 tcctttaagg tgtcactaaa aattataaaa aatagggttg acgcactttt gatgtcatgt
      241 tataataacg aagtcgtttg gatgaggtag ctaataaagc ttgattttat cgaaaaaaac
      301 ttcgaaaaag ttgttgacaa ataacaataa agatgatatg ataatcaagt tgctgctaat
      361 gagtgacaaa gacgtttaga aaaaataaac aaagaagttg ttgacattct cttctgaatc
      421 tgataagatt aaagagttgt tgaaacaaaa aga
//
LOCUS       DACDVT010000263          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDVT010000263 DACDVT010000000
VERSION     DACDVT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.285"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(36..272)
                     /locus_tag="IWL52_002876"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="IWL52_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          148 a           75 c           77 g          151 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaataagg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc
      421 atcaagaaac tccggttgct atcgttgcga a
//
LOCUS       DACDVT010000264          447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDVT010000264 DACDVT010000000
VERSION     DACDVT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.286"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(230..>447)
                     /locus_tag="IWL52_002877"
     CDS             complement(230..>447)
                     /locus_tag="IWL52_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9779341.1"
                     /translation="PGTPSQRGLNEHSNGLLRKNGLPKEMDFNQVNQGFISSVASKRN
                     HIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          141 a           51 c           82 g          173 t
ORIGIN      
        1 atttgttggt agtataattt aaaggtaata agttattctt ttattactat gtttgggttt
       61 tatagcaaag aaagtttaat aagagtttgg acctagcaaa caatattaat gaggtcatta
      121 aatcttatta tctttagtga tataatgaaa tatctacacg acaaaattat gatcttgcgt
      181 aaagttataa aatattaata aaaatatttt attaatatta tttgtcaaat taagcgagac
      241 aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa tttagtgatt
      301 ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga ttgacttggt
      361 tgaagtccat ttcttttggt agtccatttt ttcggaggag tccgtttgaa tgttcattta
      421 atccccgttg ggaaggcgtt cctgggt
//
LOCUS       DACDVT010000265          442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDVT010000265 DACDVT010000000
VERSION     DACDVT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.288"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..263)
                     /locus_tag="IWL52_002878"
     CDS             complement(<1..263)
                     /locus_tag="IWL52_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9779342.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTE"
BASE COUNT          160 a           63 c           49 g          170 t
ORIGIN      
        1 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
       61 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      121 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      181 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
      241 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
      301 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
      361 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaacataagc
      421 tatttgttta cttctgtaga at
//
LOCUS       DACDVT010000266          416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDVT010000266 DACDVT010000000
VERSION     DACDVT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.294"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IWL52_002879"
     CDS             <1..387
                     /locus_tag="IWL52_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9779343.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          159 a           74 c           65 g          118 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tttatt
//
LOCUS       DACDVT010000267          410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDVT010000267 DACDVT010000000
VERSION     DACDVT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.296"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..>410)
                     /locus_tag="IWL52_002880"
     CDS             complement(37..>410)
                     /locus_tag="IWL52_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779344.1"
                     /translation="NGEFEPLLPNNKRQDVKISGEYNFSNSALSNTRNITLKKRGRPV
                     SIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIVNTLADSYIQHRL
                     GVAKEEHIREEIQEEFEKLPK"
BASE COUNT          104 a           70 c           60 g          176 t
ORIGIN      
        1 aacttatact ttaattgaat accgcatgaa aaaaaattat tttggtaatt tttcaaactc
       61 ttcttgtatt tcttctctta tgtgttcttc ttttgctact cctagtctat gctggatata
      121 tgagtctgct aatgtattaa caattttttc gaaagaaatt ctcccttttt cactttgtaa
      181 ctccgtcata tagtcttgta aaattgagag tttgctattt aatgccggag agatcttttt
      241 aggaactgat accttgtttc ttttatcagg aattgaaact ggtcggcctc tttttttcaa
      301 tgtaatattt cttgtgttac tcaatgcact attgctaaag ttatattcac cgcttatttt
      361 cacatcttgg cgtttgttat tgggtaataa tggctcaaat tcgccattct
//
LOCUS       DACDVT010000268          394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDVT010000268 DACDVT010000000
VERSION     DACDVT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.297"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IWL52_002881"
     CDS             <1..285
                     /locus_tag="IWL52_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9779345.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DACDVT010000269          390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDVT010000269 DACDVT010000000
VERSION     DACDVT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.298"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>390
                     /locus_tag="IWL52_002882"
     CDS             <1..>390
                     /locus_tag="IWL52_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9779346.1"
                     /translation="GKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIV
                     GIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKE
                     FSNWKAISNQHDIDIYFADPGTPSQRPL"
BASE COUNT          128 a           81 c           67 g          114 t
ORIGIN      
        1 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
       61 atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt
      121 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct
      181 aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt
      241 aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga
      301 aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg
      361 gaccctggaa caccttctca acgcccatta
//
LOCUS       DACDVT010000270          385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDVT010000270 DACDVT010000000
VERSION     DACDVT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.299"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>385
                     /locus_tag="IWL52_002883"
     CDS             <1..>385
                     /locus_tag="IWL52_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9779347.1"
                     /translation="WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLR
                     KKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          142 a           69 c           72 g          102 t
ORIGIN      
        1 tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa acgtacatca
       61 aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt ttaaccttgg
      121 atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt aagcggttag
      181 tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct attacaagtg
      241 cctttaagaa actaaaagaa tacggctttt atcaagggac agaacatcgt accattaaat
      301 acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat aaattctatc
      361 gaagtttacg cactgcctct accac
//
LOCUS       DACDVT010000271          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDVT010000271 DACDVT010000000
VERSION     DACDVT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.denovo.301"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          128 a           80 c           45 g          122 t
ORIGIN      
        1 tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa tatatttatc
       61 agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt catagactct
      121 cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa attaaagggg
      181 cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct tgtgtttaat
      241 aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg tgacctccaa
      301 tttttaactc ttctatctcc catccagtat acataacctt gatacttcga ccacttatat
      361 atcgaacaca caaat
//
LOCUS       DACDVT010000272        14513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.059, whole genome shotgun sequence.
ACCESSION   DACDVT010000272 DACDVT010000000
VERSION     DACDVT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.059"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            171..752
                     /locus_tag="IWL52_002884"
     CDS             171..752
                     /locus_tag="IWL52_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9779348.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(834..1709)
                     /locus_tag="IWL52_002885"
     CDS             complement(834..1709)
                     /locus_tag="IWL52_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9779349.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1857..2189
                     /locus_tag="IWL52_002886"
     CDS             1857..2189
                     /locus_tag="IWL52_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9779350.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2301..3509
                     /locus_tag="IWL52_002887"
     CDS             2301..3509
                     /locus_tag="IWL52_002887"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9779351.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            3663..4118
                     /locus_tag="IWL52_002888"
     CDS             3663..4118
                     /locus_tag="IWL52_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779352.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            4480..4755
                     /locus_tag="IWL52_002889"
     CDS             4480..4755
                     /locus_tag="IWL52_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9779353.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            4768..6846
                     /locus_tag="IWL52_002890"
     CDS             4768..6846
                     /locus_tag="IWL52_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9779354.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            7006..8892
                     /locus_tag="IWL52_002891"
     CDS             7006..8892
                     /locus_tag="IWL52_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9779355.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            8904..9851
                     /locus_tag="IWL52_002892"
     CDS             8904..9851
                     /locus_tag="IWL52_002892"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9779356.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9871..10398
                     /locus_tag="IWL52_002893"
     CDS             9871..10398
                     /locus_tag="IWL52_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9779357.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(10461..11114)
                     /locus_tag="IWL52_002894"
     CDS             complement(10461..11114)
                     /locus_tag="IWL52_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9779358.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            11246..12088
                     /locus_tag="IWL52_002895"
     CDS             11246..12088
                     /locus_tag="IWL52_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9779359.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            12246..13142
                     /locus_tag="IWL52_002896"
     CDS             12246..13142
                     /locus_tag="IWL52_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9779360.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            13145..13858
                     /locus_tag="IWL52_002897"
     CDS             13145..13858
                     /locus_tag="IWL52_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9779361.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13878..14396
                     /gene="msrA"
                     /locus_tag="IWL52_002898"
     CDS             13878..14396
                     /gene="msrA"
                     /locus_tag="IWL52_002898"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9779362.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            14393..>14513
                     /locus_tag="IWL52_002899"
     CDS             14393..>14513
                     /locus_tag="IWL52_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9779363.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSS"
BASE COUNT         5016 a         2480 c         3016 g         4001 t
ORIGIN      
        1 tatcatgaac tatcacatga accagatttc ttgaaagagt atgccttttt cttaagagaa
       61 gaagggcagc tgaaaagagc gcaagaatta ttgacttatt atttaacact tgaaccagga
      121 gatatggagg cactgtcatt attagatgac ctaacagaga ggtgagaaaa atgctaattg
      181 atgtacggga aaagaagaat tttttaacgt ggatggtcaa tcatgtatct tttagccgaa
      241 gagaagtttt ttggatcttg aattatcttg ccaatcatga agcgatttta agcaatgtcc
      301 actttgttga aggggcgaat tttgcggatc gaggactgca gatcagtgat ctatctgttg
      361 aaggggaacc catgaaattg tttttgcagg gaaaagaatt tacggacaca gaccagattt
      421 ttcatgaaat ccgtttgaat tggaaaaagc ctttatatgt ggaatgtata ttcgaaaatg
      481 cttggcagac aagagaatat ctatctatct tagaagacaa tccattttct tcttgggatt
      541 cttcagtcag tgaagaagat actgaattaa ttgaacgata ttttcaagaa aaagaagaag
      601 aggcaaaact aaaaatgctt tatgctcaaa tcgatcaagc actagaagat ggagataggg
      661 atgctttttt gaaattatct gatgaagtta accgaaagat ccttttgcag acacaaaaga
      721 aaagtaaccg ggtaagtgaa cataacaatt agttaaaaat agtatagtac taaaaaagac
      781 aaaggtcagc tggatgtgga atctttcctc atagcctatc tttgtctttt ttcttatagt
      841 tttttcagta atcttgtctg tggcttcaaa taagtaaagc cttgcccttc cacttcatgc
      901 acattgatga ttgaaaggaa agctttttcg tcaatctcgt ggacgatcct cttagcttca
      961 gttaattcac tagagctaac gaccatatag atgatttttt ttgaattacc agagaatgcg
     1021 ccttctccag aaaggaaagt cacacctctt tgcagaccgg tcatcagtag tggtgcaatc
     1081 tcttcgcttt tgttggagac gacaagtaga ccttttgcag aatatccgcc atctaagatc
     1141 gaatcaatca cacgactgaa aacatacgaa acaatcaacg tatacatcat acgtttcaag
     1201 tcgatatagg ttaatgaagc aagtaagacg atgatatcaa atgccaatag ggaacgcccc
     1261 atagtgatgc cgtaattttg ttctaaaatc cgagcgataa tatcactgcc gcctgttgtt
     1321 ccacccatgc ggtagacgat cccgcttcct acacctgcaa ctaaacccgc aagtagagaa
     1381 acgatcagta gatcatgctg taaattgatt tctagtggaa ccctttgcca gatccataaa
     1441 aagaatgaca aagaaagtgt acctagaaca gtataggcca aggaacgttt accgaggatc
     1501 tttcctccta gtaaaatcaa tgggatgttg attaataaag tcgtataagc tggatcaata
     1561 ccaaataaag ctcttaagat caaggtgatc cctgagactc ccccttctgc taaatgatta
     1621 gcgatgttca agtaaacaag accaaatgca taaatacttg ttcctataat gataaaaaga
     1681 acttctttta ctgtttttcg tttgaccata tgaattccag ctttcaatta tgtattttca
     1741 cagaatttcc atttagaaat tccttcctaa atgtagcatg gaaattcgat tttctcaatt
     1801 gctcttatct aggatttgcg ttaaagtttt ggtaagatag ttttggaggg aaaaagatgg
     1861 ataaaaagcg ttctttatac agtatgcagc aggaagtgga tgaatatatc cagcaattca
     1921 aggtggggta tttctctcca ttagctcaga tggctcgatt aactgaagaa gtaggtgaat
     1981 tggcaagaga agtcaaccac acttatggag aaaaaagcaa aaaggcttct gaaccagtta
     2041 actcggtagc agaagaactt ggtgatgttt tattcgttac gatgatcatg gctaattcat
     2101 tgaatatcga cttaactgaa gtttttgaaa aaaacatgga aaaattcaat caaagagacc
     2161 ataatcgctt tgagcgtaag gacgagtaaa caagatgaag tggtcctggc cttgaagact
     2221 ctgaaacgga cttgtcaaac agtcaccatg caactgagtc tttatctcag gacaagaaac
     2281 acgcaggaaa ggttggaaaa atgaaattaa aacaattacc tctggaatac caaaaggcaa
     2341 ttccagttct gaaaaaatta gagaatgcag gatatgaagc ttattttgta ggtggaagtg
     2401 ttcgtgatat cttgctgaac cagccaatcc atgatgtgga tatcgcaaca agcgcatttc
     2461 cagaagaaat caaacagctt tttccaaaaa cgatagatat tgggatcgaa catggaactg
     2521 tgttggtatt agaaaatgat gaacaatatg agatcaccac atttcggacg gaatcaactt
     2581 atcaggacta ccgtagacca gatcatgtcg aattcgttcg ttcgttggca gaagatttga
     2641 aaagaagaga ttttacgatc aatgcgtttg ctttgaaaga agatggagaa atcatcgatt
     2701 tgttcgatgg tctgcaagat ctagaagaac agatcttgcg agctgtaggc aatcctcatg
     2761 agcggttcca tgaagatgcc ttacgaatga tgcgcgggtt gcgttttgtc agtcaactag
     2821 ggttcaaatt agaggaagaa accttctacg ccatcaaaga aaaccacgag ctattaggta
     2881 aaatatctgt agaacgtata aatgtggaat tcatcaagat gctcttggga aatgaacggg
     2941 ccgcgggaat ccatgcattt attgaaacag agtgctacat ttgctgtccc ggtttgaaag
     3001 aagaaggcga agcactttta cgttttgctg aattaccatc cgtcccattg aaagaagaga
     3061 gccaagcatg ggcattattg atcagccaac tggatctaaa agaaaatgag attcgttcct
     3121 ttttgaaatc gtggaagtgt tctaaccaaa tgattaaaga cgttcaagca cttgtccaag
     3181 gactacgaca acgagaaact ggagctttgg aaccattcga attgtacttc ttaggcaaaa
     3241 aacatgcact gcaagtagaa gaattgatga tctattttga cgaaacacct cagatggatg
     3301 ccgtaggaga atcctatgat cgtttaccta tcaagtcatt aagcgaatta gcagtagatg
     3361 gtaaaatctt gctagaagaa actggaaaaa aacctggtaa gtgggttagt gaagctttgc
     3421 aattagctga aaaagccgtc gtagaacaaa gaataaaaaa tgataaaaaa caggttttgg
     3481 attttttatc gaaagaaaaa ttgatttaaa aaacactaga ctgtatctgc tcagctttgt
     3541 tggctactta aagtgggcag atacgtcatt agaagaaaaa acataagatg tgaccactta
     3601 cataaatttg tgctaaactg aggtagtaca agaataaatt acatcataaa ggagtctttt
     3661 taatgagtaa agaaatgaca gcgctaaaat tttattttag aaatggagaa acctggacga
     3721 tcaaccgccg tcatatcggc gatctttgga tcaaacaaat cacaacaagt tttggacgaa
     3781 tcaatggcag tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc gaaatcttcc
     3841 atgaagggga cgcagttgcg acacatgata tcaacctagg cggactggaa atgggcatgt
     3901 ttaatcgcgc attgaaatac gaagatatcg agcgaatgga aatcctttac cgaaatggca
     3961 cacctgatct tgtgtacttc ccatatctag ataaaggaac agaaggatta gacaatcaat
     4021 atcaatcaac aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc aatccagatc
     4081 aacgtgtaga agatgtatac ggggaatttt tcgaataaat cgatttttac gcaaaaatga
     4141 ggctgtgaaa gagatgtgtc ttattactac tgataggctg tctcttccac accgtttatt
     4201 cagaataagg gactgtgaca agatttttgt cgcagcccca tttttttgtt gtgataaaag
     4261 aacaagaaag gcaggaatgg gaaagtaaac atccttttgt cagaaacttt tatctttctt
     4321 aagttttact tgatatctga tactatttgg aatagaatga ggcgttgaaa aatttatgga
     4381 aatttctaac tagcaataaa cgatgaaaca atgaaatatt tttcatccaa aaagaacttt
     4441 gctataatga tggaagtttg tgtataggag atctggaaaa tgagagagag ttttgaacaa
     4501 caaaaaaaac tgttgcatga tcgctatggc gccctttcga tggatgaccg ccgtcaaata
     4561 ctttgtaagt tgcgtaaaag aaatatattg atgtatcgtc agttggaacg tctgaaacat
     4621 gatctgcttc gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag
     4681 gttgaagtag tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa aatgcttaca
     4741 caaaataaaa aataaagggg atggacgatg gaatttgctg agttagttat tgtatttgca
     4801 gtaacgatca ccttatcgaa catagctagc cgcattttgc caatgattcc agctccactg
     4861 atccaaattt tccttggagt ggtattaggt ctgacagagt ggggacaatc aatcgatttt
     4921 gaacctgaat tgttcttagt tatgattatt gcaccattgt tatttagaga aggagaaaaa
     4981 gcagatattt cttcgattct caaaaatttc gggacaattt tgttcttggc atttggagga
     5041 gtcattttga cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca
     5101 cttgctgcct gctttgcttt tggagcagcg ttaggtccga cagatgcggt agcagtcagt
     5161 tctctttctg gacgagtgaa tatccctaaa aaggcgatgc atattttgga aggagaaggt
     5221 cttttgaatg atgcatctgg tgttactgcc ttccagtttg ctcttggtgc gctgatcacc
     5281 ggatcttttt cagcagtaaa cgcggggatg agtctagtag tttctagtat tgggggagct
     5341 ttgatcggct ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca
     5401 tcagcacaag atgtcacagg gtatctgctg atcgaacttt tgcttccttt tctagcctat
     5461 gtattggcag aggcatttga tgtatctgga atcattgctg cagtggcagc aggtattctc
     5521 caagcttctg gttttaggaa aatctctgtg tttgatgcag aattgtcgag cttgtcccac
     5581 agtacatgga caactattgc ttttacactg aatgcattag tatttatatt tttaggaatc
     5641 gaattgacgc aagtgttctc gccagtatgg ggagacggtt tatatccaaa cggtctttta
     5701 ttagcgatta tcgtattaat ctctgtcatg ctttttgtga tcagattcat ttctatcagt
     5761 cttttttatg tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg
     5821 atcttaacat ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt tattttgcca
     5881 ttttcaatca ataatatgat gttttaccag cggtctttgc ttttgtttct gacggcgggt
     5941 gtcatcttag tgactttggt catagggatc attgtcttgc ctatgctgac ggaaacagag
     6001 gaagcgaaaa gcacggattt gaatgctttg atgattttag aagaggtcgt tgaagttttg
     6061 cgaaaagaaa taaaggaaat tgatcgtaat acaaaagaat ttttagccac tgaagcagtg
     6121 atagaaaatt atcaagaacg gatacgtgat ctgtacttgg aagatttaac cgatgatgaa
     6181 aagcaagaag tacaagaaat acaagcactg attttgtcga tcgaacggga tggattggat
     6241 gaaagttatc gttcgggcaa gctttcttct aatggttatc gtttttactc ccgttttctt
     6301 tctcgattcg aacattctat cacaagccag attttgtctt ttatcggctt ttggttcatt
     6361 gttgtccgta ggttaatgcg aatcgttctc catccgaaaa tgttttggca aagaagatat
     6421 gctgatcgtc agacatttat tagtgatgaa gatatccaag aaatcagaga gacatatcaa
     6481 aaaaatactc agttgattat tgaaagttta gataacttga ctgatattta tgatgaaacg
     6541 atggttcaat tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa ctcggggaat
     6601 ttgatttctt cgtggatgat ccagcaagat agtctgttta cgaaaaaaat gctaagaggt
     6661 tattatcttg aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat cacgacgttt
     6721 tctgcgaaca gctatcgcag gaatatcaat ttgttagaat cttatacgat gaacaaacca
     6781 tctgataact tttctttccg atttgcgttt cgagcaaagt caaaaaaaac gagtccaata
     6841 aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa aacatatcag aacaggatcg
     6901 aatcggtcct gttctttttt tgagaacaca aaaagaacaa ataccaaaaa aattttcctg
     6961 aagttcacta tttttatgat acaatatcca agataaggag ggaacatgtt gaaagaatta
     7021 aaagtaactg acctcaaaaa aacttatggt gaaaaagatt tgtttgacca gatttctttt
     7081 ttggtccacg aaaaagaccg tatcgggttg attgggacga acgggacggg taaaacaagt
     7141 ctcttaaata ttattgccgg tatcgatagc agcgacggtg atcggcaaac agtgttttat
     7201 ccaactgatt accgtattgg ttatctttct caagcagctg aattttccga tgaattaact
     7261 gttctccaag cagtctttca aggaaacagc cccttgatcc aaactgttcg agcatatgag
     7321 gaagcattga tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa acgttatgct
     7381 aaagcagaag aacaaatgaa taaagaagat gcttggacta cagataccaa cgcaaagatt
     7441 attttgcaaa aattaggaat cagtgaatta gataaaaaaa tcagtacttt atcaggtggt
     7501 cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg aacccgattt actattacta
     7561 gacgaaccaa caaaccattt agattatgaa gcgatcgagt ggttggaaaa ctatttgaaa
     7621 caataccgtg gcgcactatt gatggttacc cacgatcgtt attttctaga tagagtagca
     7681 aaccgaatct tcgaactttc atttggaaaa ttatacgagt ataaaggaaa ttatgaagct
     7741 tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga aaagcgcaag
     7801 cggctttata aacaggaatt agcttggatg agggctggcg tacaggcacg tggcaccaag
     7861 cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga acttgtacca agtaaaccaa
     7921 gaagatgatt tagagctgaa cctagcgaca caacgtttag ggaaaaaagt actggaaatc
     7981 aaagatggca gttaccgtat aaatgaccaa acactgctag aacatttaga tttattgatt
     8041 caatctcggg aaagactagg tattactggt aaaaatggtg cgggaaaatc gacattgctt
     8101 aatattctag ctggcagaat tcccctggat agcggtacaa tgtctatcgg tgaaacggtt
     8161 catcttgcgt attatacaca agaaaatgaa gaaatggcac cagataaacg gatgattgct
     8221 tacttacaag aagctgctga agaagcaaaa acagcagatg gcagtcaaat cggagtagca
     8281 gaattacttg aacgattttt atttccaaga tttatgcatg ggacactgat aggcaaactt
     8341 tcaggcggag aaaaacgccg gttatttttg ttgaaactgc tgattcagca acctaatgtt
     8401 ttattattgg atgaaccgac caatgatttg gacatagcaa cattgacgat tttggaagat
     8461 tattttagaa gttttccagg tgctgtgatc actgtttccc atgaccgtta tttcttggac
     8521 aaggtagcag ataagttatt agtattccaa ggaaacggaa aacaagaact ttattacgga
     8581 aacatgtcca gttatttact gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa
     8641 ccaaaaatac agagcaagga acctgctgaa aagaagaaat tatcttacat ggaacaaaaa
     8701 gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc attgctccaa
     8761 gaagagatga atcatcaagg agataatttc actcgattac aagaattgca aaacgacgta
     8821 tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt gagtgaatgg
     8881 gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga agaaaattgt
     8941 tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc ttattcggat
     9001 accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt
     9061 ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat atccgctatc
     9121 tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac atcaaaagtc
     9181 cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca gatcctgaat
     9241 tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta accgatgatt
     9301 cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg cgtcactggg
     9361 aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga
     9421 ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt cctagcatgg
     9481 ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg ctaagctgcc
     9541 aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt gcaagttatg
     9601 cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa tttgttcaca
     9661 cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa caattgagcc
     9721 gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca gtatttgatt
     9781 ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc aaagctccta
     9841 tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc acaggatgaa
     9901 aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga tctgaaattc
     9961 ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt cgaagggatg
    10021 ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc ttcctataaa
    10081 gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca
    10141 gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct gccattttgt
    10201 acgatattat accgtacagt catccatcat gtattcgatg gagatgctta ttttccacct
    10261 gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga tgaaaaaaat
    10321 cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca
    10381 ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga gctgtcccat
    10441 ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat aaaacctgca
    10501 cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt caagctataa
    10561 aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac accagtcatt
    10621 ccaagagagt gataaagagg aacagcagcc acgacacaga gccaccccat aaaaatatag
    10681 atgatcgtag atacttttga tactctctcc cgtttatgca acgtcaacgc tttatatata
    10741 atgccaagaa tcgcaaacaa ccagattaat acaaataaag tccaacctag ccatcctcgg
    10801 atactcagca aacaaaaagg agtataactt cctgcaatca aaagaaaaat cgaactatga
    10861 tcgaatactt ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa caaagtagac
    10921 gttaaaaata aaagaatcat catcgatcca tagatagcat aagagacaat atggagggag
    10981 gaatgcaagt gggcaccttt tactaaaaga accactagtc ccgcgatact caatccagtc
    11041 ccaattccat gggtgactgc attaaacact tcatttaata tttcatacgt tcgattaaat
    11101 ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa atcattattt
    11161 gtctgttata ctaaaaatag aaattgtctt acatgtagtt taacatgaat aaggcatctt
    11221 gttaaagaaa gagagagaga aaataatgac aaatgtaaaa atcgtaacag attcttcatg
    11281 tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc tatccatcat
    11341 gatcgacgga acagtatatc ccgatgatga ccaactagac ggcgaaagat ttatggaaat
    11401 gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca ccaattggag aattcatctc
    11461 gatgtatgat gagctaggag aagacggcag tgaagtgatt tcgatccata tgacaaaagg
    11521 cttgagtgga acagtagaag cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac
    11581 ggttatcgac agtgatacca ctgaccaagg attatctttt caggtaattc gagcagctga
    11641 attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga
    11701 caatacaaaa ttatacatcg gaatatccac actagataac ttggtaaaag gtggaagaat
    11761 cagccgaaca actggtttgc tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc
    11821 ccattctgaa ttaatccctg taactaaggg gcgaggaaag aaaacgttcg ataaatggtt
    11881 taataatttg aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca
    11941 tgcggatgca aaagaattgg cgcaagaatt caaagagggt ttgcaagaat tattcccgga
    12001 catgcatatc cctgtactgc atacgaatcc ggttatcgct acgcatactg gtaaagacgc
    12061 gtttgcaatc atgtattatg tagattagtc gaaaagagga taagccctca gcctgaaagg
    12121 cttgcagctt attctctttt tctttataaa attaggaaaa taattgtttt cttttttcat
    12181 aaagactacc tttttgataa aatttttgat aacattagaa ccaatcgaat acaagaggtg
    12241 aaaaaatgaa attaacgaaa cgacatttga tcctcgtttt gttcccatta ttaattgcga
    12301 tcatcatcta tgcaggttta gctgcttcta taccgaaagc tggcccacta atgaaaccag
    12361 taactactgt gagagaagat ccaaataaaa aagaaaaact tcactacata gctatcggag
    12421 attcgctgac tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg
    12481 caaatgatct aaaagaccgt tataatttga ccgcggttga aacagaaaac tatggtgtaa
    12541 atggtgaacg aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca
    12601 atatcaaaac agctgatttt atcacactga ctgttggcgg taatgatttg atgaaagtta
    12661 tccaaaataa tttattcggg ttaacggtca attcctttaa aaagccactg aaaaactatc
    12721 aggaaaatgt cacagaacta ttggaagaat ttcgatccct caataaaacc gcacctattt
    12781 acattttagg catctacaat ccattttatt tgaactttcc tgagatcact gacatgcaga
    12841 caattgttaa caattggaac aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct
    12901 actttatccc aatcaatgat ttgctttatc aaggattgaa tcaggaagta ggtgtcacag
    12961 aagaaggaac agatcaaaca actagcacga cagaaaatct aaatggcatc aaaaataatg
    13021 tgctctatga agaagatcat ttccatccaa ataatttagg ttatcaattg atggcaaatg
    13081 cagtgagaga tcagctgatc gagacacaaa accaatggtt agagaaggag gatgatcaat
    13141 aaacatgaaa acaagatctg acgtatcttc aaacaaaaca aagaaaacaa attactggaa
    13201 attcgccttc cttatccttc ttggggtagt acttggttcg accgtatttt taggcagccg
    13261 tatttttgct aatcgggaac cagaactccc agaaatacca gctcttacgg aaagacaagg
    13321 agatcctgtc ttaacgatca attcaaataa agaaaaagtc aatcaaatca tcagcttttt
    13381 ccttagtgag tatcaaaaag atagtgatat tgaatataaa ttttatttgg aaaatgaagc
    13441 actactcaat ggtactttcg aagtactagg ttttcctatc gatttctatt tatattttga
    13501 tccgtatgtg atggaaaacg gaaatgtcca actaaaagct agaagtcttt ctatcggaac
    13561 attgagtttg ccaatcaaag atgtgatgaa tatgatcaag agaaactata aattaccaga
    13621 ttggatcgaa gtcaatacag atgatctgac tatcatgctt aggttggatc aattccgaat
    13681 gcaaaacggg atgtatatta aagcagataa aatcgactta gtaaatgatg acattcggtt
    13741 tagtctatat ttgccaaaag atacctctga tgcttcaaac aactcaaata ccaacaaaac
    13801 tagcaccagc agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg atgagtagta
    13861 ttagaggggg aatcataatg gaacgagcaa tatttgcagg aggctgcttc tggtgtatga
    13921 tccagccttt cgacacgctt cctggtatcc atacagtgat gtcaggatat actggagggc
    13981 atgtaccaga tccgacttat gaacaagtaa aaagccaaac gactggacat acagaagcag
    14041 tggagattat ctatgatcca acattgattt catatgaaca attagtagaa ctttactggc
    14101 agcaaactga tccaactgac gctttcggac aattcgagga tcgcggtgac aattatcgcc
    14161 cggtgatctt ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt
    14221 tagctgaaag tggacgtttc gaacagccaa tcgttacaaa aatcgaacca gcccaaccat
    14281 tttatctagc agaagaagac catcaagagt attataaaaa gcatcctgaa aattttaaaa
    14341 gaaaccatgc acgccgggct gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg
    14401 aagctttttt cattatttaa tgacactgcg aggcggtggc acacatgatg ctttatctgt
    14461 tttcgctaca gaagccggta aagacatcca atttcctaag cacagctcta gct
//
LOCUS       DACDVT010000273        10023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.083, whole genome shotgun sequence.
ACCESSION   DACDVT010000273 DACDVT010000000
VERSION     DACDVT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.083"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            346..1242
                     /locus_tag="IWL52_002900"
     CDS             346..1242
                     /locus_tag="IWL52_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9779364.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1385..2326)
                     /locus_tag="IWL52_002901"
     CDS             complement(1385..2326)
                     /locus_tag="IWL52_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9779365.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2452..3573
                     /locus_tag="IWL52_002902"
     CDS             2452..3573
                     /locus_tag="IWL52_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9779366.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3831..5333
                     /gene="lsa"
                     /locus_tag="IWL52_002903"
     CDS             3831..5333
                     /gene="lsa"
                     /locus_tag="IWL52_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9779367.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYRLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5400..5927
                     /locus_tag="IWL52_002904"
     CDS             5400..5927
                     /locus_tag="IWL52_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9779368.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            6139..7200
                     /locus_tag="IWL52_002905"
     CDS             6139..7200
                     /locus_tag="IWL52_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9779369.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7203..8099
                     /gene="murQ"
                     /locus_tag="IWL52_002906"
     CDS             7203..8099
                     /gene="murQ"
                     /locus_tag="IWL52_002906"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9779370.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            8113..>10023
                     /locus_tag="IWL52_002907"
     CDS             8113..>10023
                     /locus_tag="IWL52_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9779371.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGARQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDR"
BASE COUNT         3291 a         1780 c         2180 g         2772 t
ORIGIN      
        1 cacctaaagc tagcatggtg atatgtcggg agctaagttg ttttttcaat tttttatctt
       61 ccataaataa cctctttctt ttaaattcac aatcaaaatg catagtaaga ataaatagta
      121 agaataaata gtaataataa taatgactaa tgataacaaa ttttcagata attacaacta
      181 ttcagaaaac ttttgggcat attttaatgg aagaatgatt tatttcatcg tattttcata
      241 gaaatgttaa atttttataa aaataatagg aagcgtttta ctggcttttt actatttaaa
      301 caggctatca taggaaaaag tgacagagaa agtgaggcgg aacagatgaa caaaaacgac
      361 gtattgctaa ttgcgaaaaa taaatcactt tatttttttc cttccatccg ctctttagat
      421 gtttggtccg gaaaagattt actacagtcg gaattcatta aatcagctcg acaacagttg
      481 gcaaattatc agctgatcgt ttttttagat tatggatttg atatggagat ggctcgacgc
      541 gtgcgtccat ttaccaatgc aaaaatcgtg ttattttttt ggaaccactt taaagcagaa
      601 cacgaggcga tgctgaagca ggcacaagat gaaccggcaa tcgatgagat ttatcatttt
      661 gattttttag aagcgaaaga acttggacta aaacataata gttcctttta ttcgaaacat
      721 atggattttt cttctagcga accaaagacg gatctatttt ttggtgcaac aaataacggc
      781 agaaaagaac gggcagaaaa atataagcag gaatttacaa aaagaggtgt tacgaccaat
      841 tactttattc ttccgcttcg aggaaatgaa caacctggat accttactta tgaagaatat
      901 ctgaaaaaaa ccggggaatc gagggggatt ctagaactgt tgcgggaagg gcagcaagga
      961 gtgactttgc gaacgtttga atctatgtat tttcaaaaga aactagtgac agataatcaa
     1021 gccatagctc attatcagtt ttatcattct gaaaatattt ttttgcttca agaacgtgat
     1081 ttagatgaat taccggaatt tttgcatacg ccatttcaac caatcccatc agaactgttg
     1141 gaattttttg atgcagaaaa ttgggcaaaa cgttttttca ctactgactc cgctatttat
     1201 gaacaatatg aatacgatct ttcctctgtt aaaaataaat aaaagtgcca accaatcata
     1261 gatgaaaata aacaagcgca attcttatgg caacatgaga attgtgtttt tctttttgaa
     1321 ctttcatggg ggtaaaaaaa tgagaaccag ctgctgatcc agctgattct catcagagag
     1381 ccttttattc tgcgccaagg attccttctt ttgcatggac taattgggtc aaaaaggcac
     1441 aatatggtgt agcaatatca tatttttgtc cttttcgcca aacggcacca ttgatgtagt
     1501 cgatttcagt caaacgatgg tttttgatca ggtcttggta catagatggg taatgcaacc
     1561 cgatgttgtc aggatcataa gtagaagcaa tatgttggta cacttcttct tgatctaatg
     1621 tgattccttc ttttgctgcg atagaagcaa actcagatac aatcgttcga accatgccct
     1681 ctgccgcttt ttgcgcacct aattcagcaa tattacaatc caatatcgtg cataatccat
     1741 ttaatgttcc gtttacacaa gcttttcgcc aaatggaata acgaacatta tgactataga
     1801 ttggattcaa ttttgcttct tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt
     1861 ctggttccaa gttctctagt tcgatttctc catcacctaa taatgtgact tttccgggac
     1921 cagctagacc agctgtccac atcgtgatcc caaataagat atttttcttt ggtacatatt
     1981 tttccagtac atcttcatga cctaatccat tcagtaaaca caaaacataa gtattctcag
     2041 taataatgga ttggatcgaa cgaaacatat catctaattg attcgctttt gtcaaggcta
     2101 cgatcaaatc cacgtgtttg ttgctttcga tgatttcttc tggtgaatag attggcagtt
     2161 ttgcaactac ttctttgcca ttaaagtcag caatcaatcc atttgtacga atggcttcaa
     2221 tgtgagcagg ccagcgatcg atcaagacga cctcatttcc gctttgatgc aacattaatc
     2281 cgattcggct gcccattgct ccggctccag ctattgcgat tttcatgttt tttctctcct
     2341 ttattcaatg agttcccttt tagtatatag aaaaacagaa gaaaatagca ttgttttgtt
     2401 tgaaaaaagt gaaaaaaagt gtcttactta agaagaaagt gagggatata gatggatatt
     2461 ttggcggcaa tcgattcatt taaaggaagt gcttcttcat ttgaattaaa tcaggcagtt
     2521 ctaaatggtt gggaagaccg taaaggaaga aaaatcaatg tgccgattgc agatggcgga
     2581 gaaaatactg cagaagcaat ctattacgcg ctcgggggaa cctggcgtac gattgatggg
     2641 atggatctct tatttcgtca gcgaaaaatc agttatttat tgacgaactg gcaaggaaaa
     2701 aataccgcag tcattgaatc tgcacaagta ttaggattag atttacttga taaaccaacg
     2761 gatcaaacca tccgacttgc ttctagttat gccttgggag agcttatcaa agatacactg
     2821 cttcaagaag tggaacaaat cattgtcact ttaggaggaa gcggatgtag cgatggtggt
     2881 ctaggattac tccaatcttt gggtgcttct ttagaagggt acacagaagg aaatcctttg
     2941 ttatctacta gaaaacttca tttggatcga aggaacgttc ttgtttctaa agtaaaagtg
     3001 atcatcgctg cagacgtgac caacccatat acaggtcaac aaggagctgc ttggcaattt
     3061 ggaagacaaa aaggaggaac ggatacgacg ttagctttct tggataaaca agctgaaaag
     3121 attgctcagc agataaaaga tgtttgggag gtggatttag atgacatccc aggaagcgga
     3181 gcagcaggcg gtattggcgg agcccttttt ttgttaggag cgaagatggt ctctggtttt
     3241 gagttggtca gccaaatcac tggattagaa aaacagatcg aacaagcgga tgtcatcatt
     3301 acaggagaag ggcggattga tcagcaaaca atccacggaa aagttccata tggcgtagca
     3361 gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccgagag
     3421 ctggaaggac tctttgattt tctccccatt gtcttaagca ttcaattagg accaatcgat
     3481 ttagaggaag caatggaaca caggcaaaca ttagaaaaaa cagaaatctt aggacagaca
     3541 ctcagcagat ttttcaacat tctcccgaag tagttgacaa aaacaatttc tttatgctat
     3601 cgtaatttca aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga
     3661 tgaaagctgc attcagactg taccttgtgg agacaaggga gcagtgtgca gattttcggg
     3721 attggcttgg ttctttcgat gtatgggtct ttttgtgtgc cccaaccctt gcatcctctt
     3781 tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaata atgtctaaaa
     3841 tcgaaataaa aaatctgaca ttcggctatg acagccaagg cacattatta tttgaacaag
     3901 ccaatctaaa ctttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa
     3961 agacaacttt actgaatatc ctgcaaaaca aactccctta tcaagggcaa gtgatccatc
     4021 agcaagcgtt tgtctatttc ccgcagcaaa caaaagataa agagcgtttg acctattatg
     4081 tgttaaatga gattatggat tttgagatat gggaaattga aagagagctc caactgatgc
     4141 aaacagatcc tgaaatctta tggagagaat tcagcacact atcaggagga gagaagacaa
     4201 aagtcctact ggcactctta tttgtggatg acactcattt cccgttaatc gatgaaccaa
     4261 cgaatcattt ggatatctct ggcagaaaac aagtagcggc ttacttgaaa aagaaaaaac
     4321 aaggattcat cgtggtgagt catgatcggg gatttatcga tgaagtagtg gaccatgttt
     4381 tagcaatcga aaagagccaa ctggaacttt atcaagggaa tttctctatc tatgaagaac
     4441 agaaaaaact tcgtgatgaa tttgaaatag ctaaaaatga aaaattgaaa aaagaagtca
     4501 gcaggctaaa gaaaacagca gctgaaaaag cagaatggtc tcgttcccga gaaggggaca
     4561 aaacaaagaa aaaagtcgga ttcatcgata cggaatctag gcgagtgaat aaaggagcag
     4621 taggcgctga tgctgcacgg acgatgaaac gatctaaagc aatcgtgaat cggatggaaa
     4681 cccagatcag tgagaaagaa aaattattaa aagatatcga gtatatcgat gctttgacga
     4741 tgaatagcca agtgtctcat cataaacggc tgttaagcgt agaaaatctt caattagggt
     4801 atgaaaatct gttattcgag ccagttcatt ttacgatcga acctcatcag cggatggcaa
     4861 tttcaggtcc taacggcgcg ggaaagtcat ccattatcca ttatcgtctg gggacattca
     4921 acggcaaggt tgtaggagaa aaatcccagc ccaaacattt gaccattagt tacgtacgcc
     4981 aaaattatga agacaatcga gggacattgg cggaattcgc gaagaaaaac cgagtagact
     5041 atcaagcatt tttgaataat ctccgaaagc ttgggatgga aagagatgtt tttcataata
     5101 aaatcgaaca gatgagtatg ggacaacgga aaaaagtgga attggctaaa tctttatccc
     5161 aaccagctga gctctatata tgggatgaac cattgaatta tttggatgtc ttcaatcaag
     5221 aacaattaga acaactgatt ttgaacgtga aacctgctat gttactagtg gaacatgatc
     5281 aaacctttct agataaagta tctactgaga ttatttctct tgagaaaatc taaaccttgt
     5341 aatctcaaag acaacaaggt atggttaagg agaactttgt caacgaagga gagaatcaca
     5401 tgctttatgt aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcgggc
     5461 gagcagatgt gccgctgact gaaaaaggac agatgcaggc acaggcacta gcagaaaagg
     5521 tagtagattt gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag
     5581 atacagcaca agcagtagtt gatcgtctca atttgccgat gaccgctgat gagcgattgg
     5641 tagaaatgga ttttggggat tatgacggac tgccaagcaa agatgaaaac tttcaaaaag
     5701 cgcgtttagc gtttgctgta cgtttcccaa atggagagtc ggtcttagat gtgtacgcac
     5761 ggatcgttcc tttattgaaa gaatgcatgg aagacgaaga aaatgtctac ttgttagtct
     5821 gtcataatgc cttgataaga gtgatcaatg cttattttca tccaatgcct aatgaagggt
     5881 tctttacatt catggtagac aatacagaat taatttctta tgagtaatta gacttttgtg
     5941 ttatttaaat aaaatgtttc aaggcactgc ttttgcttag cagtgccttt tttgtggctg
     6001 tgtcaattct tttggcatta taaatccaat ttttagaaat aaaatttaat tattttaaca
     6061 aaagtattga aattttgttt ctttagatat atactctagt catagaaatg aagacggata
     6121 cagaaaaaga gggataatat gtttggcttt tcaatattta tgaatgattc tttgtcagaa
     6181 gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc
     6241 atgcatatac cggaagatga tgtttcggct tatcaaaatc gattgatcga actaggaagc
     6301 gaagcgaaaa agaataaact agagttgatg gtcgatattt caggagaggc attgaaacga
     6361 gctggctttt cattagaaga tctaacaccc ttgaaagcaa ttggtgtaac agggttaaga
     6421 atggattatc atatttctaa tcagcagatc gccgactggt cacatcaatt gaaaatcagt
     6481 ttgaatgcca gcacgatcac gcctaatgat atcgatgagt tgaaagaagc agcggctgat
     6541 ttctcacaga ttgaagcttg gcataattat tatccacgtc ttgaaacagg gttggacaaa
     6601 gaatggtatc aggaaaaaaa tcaatggctc aaaactcaag gattccttgt acaaggcttt
     6661 gtaccaggag atacagagtt gagaggtcca ctgtatcaag gattaccaac tttggaagaa
     6721 catcgaggga tccatcctct ggcaggtgct cttgatctat tagcaagtaa cacggatatt
     6781 gtttatatcg gagatgcagg tctgtcagaa cacgttcagg agcaatttgc ttctttccaa
     6841 aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagtttttta cgaatatata
     6901 ttaggaaaac atacgaatcg gcaagatgat gcaagagatg tcatacgaag cgcagatgcg
     6961 cgttttcgag agattccgcc aatcccagta agaaatacag ctaagcgtat gaaaggatct
     7021 attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aactaaatat
     7081 gatttgccag cggatgaaaa agtgaatgta gtagccaaag tcatcaaaga agaactaccg
     7141 ctgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa
     7201 aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg
     7261 gattagatga aatgagtatt tctcaagcag tccaactcat gaacaaagaa gatcagaaag
     7321 tggccgctgc tgttgaaaaa gaactgccaa gtatcgagcc agtgatcgcc aacacgattg
     7381 aatcctttaa aaaaggtggt cgcttgattt atatgggagc tggaacgagt ggtagacttg
     7441 gtgtattaga tgctgctgaa tgtgtaccga cattcggtgt agaaccggaa atggtcatag
     7501 gcctaattgc tggtggagaa gaagcaatga ctgtagccgt cgaaggagcc gaagattctt
     7561 atgaattagg gcaagaagat ctaatcaagc tgaatttaac aaaaaatgat atagttattg
     7621 gtatcgcagc tagcggtcgt acaccatatg tgattggtgg attagattac gcaaaatcga
     7681 ttggtgctgt tacaggaacg atttcttgca ataaacaagc agaaatcagc gagcatgcgg
     7741 atttcccaat cgaagtcgat gcaggaccag aatttttgac aggctctact cgattgaagt
     7801 caggaacagc acaaaaactg attttgaata tgatttctac tatcagtatg atcggtatcg
     7861 gtaaagtatt caataacttg atggtcgacg tcaaaccaac aaatgaaaaa ctaattgaac
     7921 gctcaaaacg aatcatcatg caagcgacag aagctgacta cgaaacagct gaaaagtact
     7981 ttgaggaagc agatcaaaat gtcaaactgg ctatcgtcat gattttgaca aatagtgata
     8041 aagaggcagc tcaagcaaaa ttagctgaag caaacggttt tgtaaaagga accttatgag
     8101 ggggaaaaga ccatggcaaa aaacgaattg actttggaag aactggcaaa acaaatttat
     8161 caaggagcag gcggtatggg caatgttgaa agcattgtcc actgcatgac tcgtgtacgt
     8221 atgaaggtca tcaatgatga tcttgtaaat gtctcggaat taaaagcgat tccaggagta
     8281 cttggcgtag tagaggatga acagctccaa gtcatcatcg gacctgggaa agtgaacaaa
     8341 gtagcgcagg aaatggttga cttagcaggc gtgaaattag gagaaaaact tccttctgct
     8401 agtgcaacta cagcaagttc ttctgcatct ggaaaagacc gtgtaaacga acgggcacaa
     8461 gaaatgaaag cggcacaaaa atcgaaacaa aaaccctcta aaatcaaatc ggtattaaaa
     8521 gacatctcta atatctttgt accaatgatc cctgcatttg tcggcactgg gatcgtggca
     8581 gggattgccg cagttttagc aaacttagtc acagctggaa cactagacgc aggaacatgg
     8641 caacaatata tcgatgtgat gaacatcttg aaaaacggga tgtttagcta tctagttatc
     8701 tatacaggta tcaatgctgc acaagttttt ggtgcaacgc caacgcttgg tggagttatc
     8761 ggggctgttg tgatgctgac agggatgaat ccagaagcac cactgaaaaa cttgtttacc
     8821 ggtgaagctc ttgcaactgg acaaggcggg atcttaggtg ttatcttcgc agtttggtta
     8881 ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt
     8941 acgccaactc tttccctgat tgccatcggt ctgatcgaaa ttttcttcat catgccttta
     9001 gcagggttca tttctgacgg catggtcggt gggatcaact gggtacttgg tgttggagga
     9061 gcatttgccg gcttcgttct aggtacatta ttcttgccaa tggtcatgtt cggactacat
     9121 cagatcttga cgccaatcca tgtacaaatg attgatgaaa ctggtcgtac cttattgctt
     9181 ccaatccttg cgatggccgg agcaagacaa gtgggtgcag cattggcatt atggatccgc
     9241 tgcaaaaaag acaaagaatt aacagaaatg atcaaagggg ctttaccagt cggaatctta
     9301 ggaatcggcg aaccattgat ttacggagta acattacctc taggacgtcc atttattact
     9361 gcttgtatcg gtggaggtat cggtggggca gtgatcggaa tgatcggaaa tgtcggagca
     9421 atcgctattg gaccttcagg agccgctttg attccattga tctctgatgg taaatggtta
     9481 ggctatatcc ttggtttatt ggctgcttac gctggtggct tcgttgctac attcttcttc
     9541 ggtattccta aagaccgttt agaaaaagaa gaactagcga ccgaaacagc cacagtaaat
     9601 gaagtacctg aaaaagcagt acctcaagct tcagctgaaa gtgtgactat cttatcatca
     9661 gttgctgacg gaacagttgt ttctttagat aaagcaagtg acccagtttt tgcacaaaaa
     9721 atgatgggtg aaggttactt tgtagaacca gaaaacggac atatctattc cccggttact
     9781 ggagtaataa gttctgtttt cccaacaaaa catgctatcg gcattacgac accaaatggc
     9841 ttagaaatct tgcttcacat gggcatcaac acagttgact taggcggtac gccattcgat
     9901 gtgaaagtaa cagaaggaca gcaagtgaca cctgatacgc ttgtagcaga agctgactta
     9961 gaagcaatca aagcagcaga caaagaaacc tccatgatcg tcttggtaac caatatggat
    10021 cgt
//
LOCUS       DACDVT010000274         4993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.125, whole genome shotgun sequence.
ACCESSION   DACDVT010000274 DACDVT010000000
VERSION     DACDVT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.125"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            245..1213
                     /locus_tag="IWL52_002908"
     CDS             245..1213
                     /locus_tag="IWL52_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9779372.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IWL52_002909"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IWL52_002909"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9779373.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IWL52_002910"
     CDS             2243..2851
                     /locus_tag="IWL52_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9779374.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /gene="vanY"
                     /locus_tag="IWL52_002911"
     CDS             3279..4190
                     /gene="vanY"
                     /locus_tag="IWL52_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9779375.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IWL52_002912"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IWL52_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP9779376.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         1590 a          858 c         1123 g         1422 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacta tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcc
//
LOCUS       DACDVT010000275         4210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.136, whole genome shotgun sequence.
ACCESSION   DACDVT010000275 DACDVT010000000
VERSION     DACDVT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.136"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..188
                     /locus_tag="IWL52_002913"
     CDS             3..188
                     /locus_tag="IWL52_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9779377.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="IWL52_002914"
     CDS             203..1072
                     /locus_tag="IWL52_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9779378.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="IWL52_002915"
     CDS             1053..1787
                     /locus_tag="IWL52_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9779379.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="IWL52_002916"
     CDS             1820..2728
                     /locus_tag="IWL52_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9779380.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3267
                     /gene="sat4"
                     /locus_tag="IWL52_002917"
     CDS             2725..3267
                     /gene="sat4"
                     /locus_tag="IWL52_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP9779381.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3360..4154
                     /locus_tag="IWL52_002918"
                     /note="aph(3')-IIIa"
     CDS             3360..4154
                     /locus_tag="IWL52_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9779382.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         1442 a          626 c          962 g         1180 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     3001 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     3061 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     3121 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     3181 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     3241 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     3301 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     3361 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     3421 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     3481 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     3541 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     3601 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     3661 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     3721 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     3781 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     3841 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     3901 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     3961 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     4021 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     4081 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     4141 atgaattgtt ttagtaccta gatttagatg tcggttctgt tgcaaagttt taaatctact
     4201 atcaaataag
//
LOCUS       DACDVT010000276         2658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.159, whole genome shotgun sequence.
ACCESSION   DACDVT010000276 DACDVT010000000
VERSION     DACDVT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.159"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..196
                     /locus_tag="IWL52_002919"
     CDS             <1..196
                     /locus_tag="IWL52_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9779383.1"
                     /translation="NLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHGT
                     DIDAACGQLRSKQMKKTVAK"
     gene            complement(389..1492)
                     /locus_tag="IWL52_002920"
     CDS             complement(389..1492)
                     /locus_tag="IWL52_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9779384.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2045..2593
                     /gene="aac(6')"
                     /locus_tag="IWL52_002921"
     CDS             2045..2593
                     /gene="aac(6')"
                     /locus_tag="IWL52_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9779385.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          864 a          465 c          514 g          815 t
ORIGIN      
        1 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
       61 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
      121 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
      181 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
      241 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
      301 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
      361 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
      421 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
      481 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
      541 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
      601 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
      661 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
      721 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
      781 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
      841 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
      901 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
      961 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
     1021 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
     1081 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
     1141 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
     1201 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
     1261 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
     1321 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
     1381 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
     1441 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
     1501 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
     1561 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
     1621 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
     1681 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
     1741 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
     1801 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
     1861 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
     1921 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
     1981 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
     2041 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
     2101 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
     2161 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
     2221 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
     2281 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
     2341 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
     2401 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
     2461 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
     2521 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
     2581 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
     2641 aatttacgaa aattactt
//
LOCUS       DACDVT010000277         2551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.162, whole genome shotgun sequence.
ACCESSION   DACDVT010000277 DACDVT010000000
VERSION     DACDVT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.162"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..459
                     /locus_tag="IWL52_002922"
     CDS             <1..459
                     /locus_tag="IWL52_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9779386.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="IWL52_002923"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="IWL52_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9779387.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="IWL52_002924"
     CDS             1346..2500
                     /locus_tag="IWL52_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9779388.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          453 c          535 g          683 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa t
//
LOCUS       DACDVT010000278         2350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.170, whole genome shotgun sequence.
ACCESSION   DACDVT010000278 DACDVT010000000
VERSION     DACDVT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.170"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..1948
                     /gene="tet(S)"
                     /locus_tag="IWL52_002925"
     CDS             8..1948
                     /gene="tet(S)"
                     /locus_tag="IWL52_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP9779389.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2036..2203
                     /locus_tag="IWL52_002926"
     CDS             2036..2203
                     /locus_tag="IWL52_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9779390.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
BASE COUNT          853 a          350 c          425 g          722 t
ORIGIN      
        1 cttttgaatg gaggaaataa aattgaaaat tattaatatc ggtatcttag cacatgttga
       61 tgcaggaaaa actactttga cagaaagctt actatacagt agcggagcaa ttaaagagtt
      121 aggaagtgta gatagcggta caacgaaaac ggatactatg tttttggaac gccagagagg
      181 tattactatt cagaccgcaa taacatcttt tcaacgggaa aatgttaaag taaatattgt
      241 agatactcct ggacacatgg attttttggc agatgtatac cgttcattat ctgttttgga
      301 tggagctatt ttgctaatct ctgcaaaaga tggagtacag tcacaaactc gtatactatt
      361 ccatgcactt agaaagatga acatacctat aatatttttt attaacaaaa ttgatcaaaa
      421 tggaataaat ttgccagatg tttatcaaga tattaaggac aaactttctg acgacatcat
      481 aattaagcag actgtgaatc taaatttgaa accttatgta atagattata ctgaaccaga
      541 acaatgggag acagtaattg tgggaaatga ttatttatta gaaaaatata ccattgggaa
      601 aacattgaat attgcagaac ttgaaaagga ggaaaacgaa agaattcaaa gttgctcctt
      661 atatcctgtt tatcacggaa gtgcaaagaa taatattgga attaaacaac ttatagaggt
      721 aattactagc aaattatttt cacccacaca actcaattca gataaacttt gtggaaatgt
      781 ttttaaagta gaatattcag atgatggtca acggcttgtc tatgtacgtc tttatagtgg
      841 aacgctacat ttgcgagact cagtcaatat atcagaaaag gaaaaaataa aagttacaga
      901 aatgtatact tcaataaatg gagaattacg ccagatagat aaggcagagc ctggtgagat
      961 tattatttta aaaaatgagc ttttaaaact aaataacgta cttggagata aaaaaagatt
     1021 accacataga gaaattcttg agaatcctct tcctatgtta caaacaacaa ttgaaccatg
     1081 taaatcagta caaagagaaa agttactaga tgcacttttt gaaatatccg atagtgatcc
     1141 ccttctacaa tattatgtag atacagtaac tcacgaaatt gtgctatctt ttttaggtga
     1201 ggtccaaatg gaggtaactt gtactctgat tcaagaaaaa tatcatattg agatagaaac
     1261 aagaaaacca actgtcattt atatggaaag accattaaaa aaatctgaat ttaccattga
     1321 tatcgaagta cctccaaatc ctttctgggc ttctattggt ttatctgtaa caccacttcc
     1381 tttgggtagt ggcattcagt atgagagcct ggtttctcta ggttatttaa atcaatcatt
     1441 tcaaaatgca gttatggaag gtatacgcta tgggtgtgaa caaggattgt acggttggaa
     1501 attaacagac tgtaagatct gttttaagta tggtctatat tacagccctg tcagtacgcc
     1561 agcagatttc cgaatgcttg cgcctattgt actagagcag gcttttagaa agagtggtac
     1621 agagttatta gagccatatc ttagcttcga aatttatgta ccacaagaat atctttcgag
     1681 agcatataat gatgcttcca aatattgtgc aaatatttta aatactaagt taaaaggtaa
     1741 cgaggtcatt ctcattggtg aaattccagc ccgttgtatt caagagtatc gaaacagttt
     1801 aactttcttt acaaatggac gcagtgtctg tttaacagag ttaaaaggtt atcaggttac
     1861 taacattaag tctgctttcc aaccacgtcg tccaaataat agaatagaca aagtaaggca
     1921 tatgtttaat aaaatcaact tacattgata gtgaactaaa gagaaatttt taaaatgaat
     1981 attgagttgt taattagaac aggaggatat agatattgta aatactaggt ggatactgtg
     2041 ccctatatgt ggcaataaga cccgtttaaa aatgagggaa gatactgaat taaaaaattt
     2101 ccctctattt tgtcctaaat gcagacaaga aattttaatt gaaataacga agtttagaat
     2161 aactgtaatt acagagccag acgcaaagac gcagagccga taattcgaga tatcaaaatc
     2221 tcattttatc ggctttttct tttctatgca taccattgag cttagttctt gatatccttt
     2281 ataagaaaat aagtgtactg attttttttg cagtagctgt attacagtag acatccctca
     2341 agcataaaaa
//
LOCUS       DACDVT010000279         1966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.179, whole genome shotgun sequence.
ACCESSION   DACDVT010000279 DACDVT010000000
VERSION     DACDVT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.179"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            393..1871
                     /gene="msr"
                     /locus_tag="IWL52_002927"
     CDS             393..1871
                     /gene="msr"
                     /locus_tag="IWL52_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9779391.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT          695 a          320 c          410 g          541 t
ORIGIN      
        1 gttcgtttag caccagaaaa gatcactacg ttcgaataaa aggttttcag ttactattca
       61 gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa
      121 tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag
      181 acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa
      241 taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa
      301 aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt
      361 gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa
      421 atctacaagt cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc
      481 agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac
      541 taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact
      601 acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc
      661 atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa
      721 agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa
      781 caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg
      841 gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct
      901 gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc
      961 aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa
     1021 aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt
     1081 ccagtaaaaa gaaaaagcaa caaatcagac cggatcggtt gtcttcctct aaacaaaaag
     1141 acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa
     1201 taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc
     1261 ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga
     1321 gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg
     1381 gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa
     1441 tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat
     1501 ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc
     1561 gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat
     1621 taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg
     1681 tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga
     1741 agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg
     1801 agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga
     1861 gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg
     1921 cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtt
//
LOCUS       DACDVT010000280         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31947_2015
            SAMN12639657-rid9881993.guided.227, whole genome shotgun sequence.
ACCESSION   DACDVT010000280 DACDVT010000000
VERSION     DACDVT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639657
            Sequence Read Archive: SRR10030664
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741969.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 07:30:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,929
            CDSs (total)                      :: 2,864
            Genes (coding)                    :: 2,794
            CDSs (with protein)               :: 2,794
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 33 of 70
            Pseudo Genes (incomplete)         :: 35 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 13 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639657-rid9881993.guided.227"
                     /isolate="E31947_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            108..191
                     /locus_tag="IWL52_002928"
     CDS             108..191
                     /locus_tag="IWL52_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP9779392.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            316..1017
                     /gene="erm"
                     /locus_tag="IWL52_002929"
     CDS             316..1017
                     /gene="erm"
                     /locus_tag="IWL52_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP9779393.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTGSVAKF"
BASE COUNT          396 a          170 c          159 g          310 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattt tgtgtataat aggaattgaa
       61 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
      121 aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
      181 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      241 agataactaa aattacaaac aaatcgttta acttctgtat ttatttacag atgtaataac
      301 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      361 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      421 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      481 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacatt
      541 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      601 aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      661 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      721 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
      781 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
      841 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
      901 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
      961 aaacacgcca aagtaaacaa tttaagtacc gttactggtt ctgttgcaaa gttttaaata
     1021 aagaataaaa tccct
//
